AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene64753

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64753.t1 MTR_5g021770 97.814 366 8 0 1 366 1 366 0.0 746
MS.gene64753.t1 MTR_5g021760 96.995 366 11 0 1 366 1 366 0.0 740
MS.gene64753.t1 MTR_4g120030 24.927 341 192 13 23 344 110 405 2.74e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64753.t1 AT5G66280 83.989 356 53 3 13 366 5 358 0.0 628
MS.gene64753.t1 AT3G51160 81.267 363 66 2 4 366 10 370 0.0 627
MS.gene64753.t1 AT2G47650 24.713 348 195 14 23 346 118 422 1.12e-11 66.2
MS.gene64753.t1 AT3G62830 24.198 343 195 13 26 346 121 420 3.43e-11 64.7
MS.gene64753.t1 AT3G62830 24.198 343 195 13 26 346 121 420 3.43e-11 64.7
MS.gene64753.t1 AT2G47650 25.989 354 189 17 23 346 118 428 4.53e-11 64.3

Find 95 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTGGCGGTGTTGGGGTTT+TGG 0.163598 5.2:+12978790 None:intergenic
ACTGAAGTTGATAATCTTAA+AGG 0.192272 5.2:-12977675 MS.gene64753:CDS
CTTCAGAGTAAGTTGTTTCT+TGG 0.283233 5.2:-12977903 MS.gene64753:CDS
TTTCCAACCAAGAACTTTCT+TGG 0.301061 5.2:+12977638 None:intergenic
AGTAAAGCCAAGAAAGTTCT+TGG 0.319296 5.2:-12977645 MS.gene64753:CDS
TCCGTCTTGGCCGGTGATTC+CGG 0.325812 5.2:+12978721 None:intergenic
CGTTTGTTGTTGGCGGTGTT+GGG 0.331753 5.2:+12978783 None:intergenic
CCGTTTGTTGTTGGCGGTGT+TGG 0.337657 5.2:+12978782 None:intergenic
AGTTTGACGAACGGCGAATT+AGG 0.352603 5.2:+12978651 None:intergenic
GCTGGATCTTCTGAAATGTT+TGG 0.354742 5.2:-12978341 MS.gene64753:CDS
ACCTAAGTGCGTCGAGAGAT+TGG 0.376609 5.2:-12977878 MS.gene64753:CDS
TGTTGAGGAGGAATTCTGTT+AGG 0.384121 5.2:+12978693 None:intergenic
CCTAAGTGCGTCGAGAGATT+GGG 0.389278 5.2:-12977877 MS.gene64753:CDS
TTGGTTATGTTGGATTGAAT+TGG 0.394470 5.2:-12977737 MS.gene64753:CDS
ACAACATCGGCAGTGTAATC+AGG 0.400097 5.2:+12978446 None:intergenic
GCGAGGTTGTAAACTTCGTC+AGG 0.409532 5.2:+12978503 None:intergenic
GTACCAATGCGCAGCGACTT+TGG 0.414772 5.2:+12978256 None:intergenic
AGGAAGATTACTAGAGTTGT+TGG 0.423133 5.2:-12977942 MS.gene64753:CDS
GTGAATTACCGTGAAGCATA+TGG 0.425833 5.2:-12978032 MS.gene64753:CDS
CCAACACCGCCAACAACAAA+CGG 0.429296 5.2:-12978782 MS.gene64753:CDS
TGATATTGAGATGGCTAAGA+AGG 0.440336 5.2:-12977571 MS.gene64753:CDS
TCGAGAGATTGGGGATTTGC+CGG 0.445475 5.2:-12977867 MS.gene64753:CDS
ACGCTTCCTCTCTCCGTCGC+TGG 0.449912 5.2:-12978541 MS.gene64753:CDS
GAGAAAGTCCTTGTTGATGC+TGG 0.452535 5.2:-12977549 MS.gene64753:CDS
CTGTTAGGTATGATCCGTCT+TGG 0.465366 5.2:+12978708 None:intergenic
CGATCTCATATCGATGCAAC+AGG 0.466501 5.2:-12978389 MS.gene64753:CDS
AAACGACTTCGTTTCATCCT+CGG 0.473906 5.2:-12978295 MS.gene64753:CDS
AGAGGAAGCGTCAGAGAGAT+CGG 0.476997 5.2:+12978553 None:intergenic
CCTCCAAAGTCGCTGCGCAT+TGG 0.478635 5.2:-12978259 MS.gene64753:intron
GTGGTGTCGCCGTTTGTTGT+TGG 0.483145 5.2:+12978773 None:intergenic
AGTTCTTGGTTGGAAACCTA+AGG 0.486640 5.2:-12977631 MS.gene64753:CDS
GAGGAGAGAATTTCGTTACA+AGG 0.489476 5.2:-12977962 MS.gene64753:CDS
GATGTGGTTGATGCTACAAC+AGG 0.492498 5.2:-12977829 MS.gene64753:CDS
CCCAATCTCTCGACGCACTT+AGG 0.494377 5.2:+12977877 None:intergenic
TGAAGAGCTTGTTAGAATGA+TGG 0.497175 5.2:-12977601 MS.gene64753:CDS
AAGCCAAGAAAGTTCTTGGT+TGG 0.498900 5.2:-12977641 MS.gene64753:CDS
AGCTCTTCAAAACTAACCTT+AGG 0.505928 5.2:+12977615 None:intergenic
CACATACGGTACTATCAAGC+TGG 0.507207 5.2:-12978359 MS.gene64753:CDS
GCATTAATCACCGGAATCAC+CGG 0.526480 5.2:-12978731 MS.gene64753:CDS
TTGTTGGAAGAATCAAGATC+GGG 0.530932 5.2:-12977926 MS.gene64753:CDS
GCGACTTTGGAGGCGGCGTA+AGG 0.533507 5.2:+12978269 None:intergenic
ATGCGCAGCGACTTTGGAGG+CGG 0.536171 5.2:+12978262 None:intergenic
TTAAGATTATCAACTTCAGT+AGG 0.538937 5.2:+12977678 None:intergenic
GTCGAGCCAGCGACGGAGAG+AGG 0.549312 5.2:+12978535 None:intergenic
TAGGTATGATCCGTCTTGGC+CGG 0.550384 5.2:+12978712 None:intergenic
GTGTCGCCGTTTGTTGTTGG+CGG 0.555514 5.2:+12978776 None:intergenic
AGAAAGTCCTTGTTGATGCT+GGG 0.563986 5.2:-12977548 MS.gene64753:CDS
CGGAGTAGCCTTTGTTGAGG+AGG 0.568302 5.2:+12978681 None:intergenic
CCAATGCGCAGCGACTTTGG+AGG 0.569706 5.2:+12978259 None:intergenic
GTTGTTGGAAGAATCAAGAT+CGG 0.578552 5.2:-12977927 MS.gene64753:CDS
ACCGGAATCACCGGCCAAGA+CGG 0.579451 5.2:-12978722 MS.gene64753:CDS
CTAAGTGCGTCGAGAGATTG+GGG 0.581879 5.2:-12977876 MS.gene64753:CDS
ACTGCCGATGTTGTTGCTAC+CGG 0.584186 5.2:-12978437 MS.gene64753:CDS
TTCAAGAAGACGGAGCGCAC+CGG 0.584604 5.2:+12978418 None:intergenic
GACTTTCCGCAGTGGAGGAG+TGG 0.587386 5.2:+12978754 None:intergenic
ATCGGACGGCTTCAAGAAGA+CGG 0.590667 5.2:+12978408 None:intergenic
ACGAACGGCGAATTAGGCCG+TGG 0.595994 5.2:+12978657 None:intergenic
GGTTGATGCTACAACAGGAA+AGG 0.599717 5.2:-12977824 MS.gene64753:CDS
CATCGCTTCAACATAATCAC+CGG 0.605949 5.2:+12977848 None:intergenic
TGATGATTATGTTGTGGCGA+CGG 0.608282 5.2:-12977799 MS.gene64753:CDS
GGACGGAGTAGCCTTTGTTG+AGG 0.608416 5.2:+12978678 None:intergenic
GGCGACGGAGGATTCGCATA+CGG 0.610465 5.2:-12977784 MS.gene64753:CDS
GTTGATGCTACAACAGGAAA+GGG 0.610852 5.2:-12977823 MS.gene64753:CDS
GTTTGTTGTTGGCGGTGTTG+GGG 0.611035 5.2:+12978784 None:intergenic
CGGAAAGTCGCATTAATCAC+CGG 0.613778 5.2:-12978740 MS.gene64753:CDS
ACGGCGAATTAGGCCGTGGA+CGG 0.616647 5.2:+12978661 None:intergenic
GTGATTATGTTGAAGCGATG+TGG 0.617088 5.2:-12977845 MS.gene64753:CDS
AATGCGACTTTCCGCAGTGG+AGG 0.619549 5.2:+12978749 None:intergenic
ACAGAATTCCTCCTCAACAA+AGG 0.619656 5.2:-12978689 MS.gene64753:CDS
GTGTGTTGAAGTTTGACGAA+CGG 0.622272 5.2:+12978642 None:intergenic
CAACAGGAAGATCTCACATA+CGG 0.624247 5.2:-12978373 MS.gene64753:CDS
AACAAAGGCTACTCCGTCCA+CGG 0.626974 5.2:-12978674 MS.gene64753:CDS
GGATAGTGTCGAGCCAGCGA+CGG 0.629653 5.2:+12978528 None:intergenic
CAATGTACCCAGCATCAACA+AGG 0.631014 5.2:+12977541 None:intergenic
TGCATCGATATGAGATCGGA+CGG 0.632798 5.2:+12978394 None:intergenic
GGTTGATAATGATATTGAGA+TGG 0.632801 5.2:-12977580 MS.gene64753:CDS
CTGTTGCATCGATATGAGAT+CGG 0.632916 5.2:+12978390 None:intergenic
TTATGTTGGATTGAATTGGA+AGG 0.637434 5.2:-12977733 MS.gene64753:CDS
AACGAAGTCGTTTCAGACTG+CGG 0.644350 5.2:+12978305 None:intergenic
TGAGCTTTGTGAGTGTCGTG+TGG 0.658563 5.2:+12978593 None:intergenic
AACCGCGACGTGAGACTGTG+CGG 0.665940 5.2:+12978481 None:intergenic
CGCCGCACAGTCTCACGTCG+CGG 0.668155 5.2:-12978483 MS.gene64753:CDS
CGAGGTTGTAAACTTCGTCA+GGG 0.672942 5.2:+12978504 None:intergenic
AATCATGAATCACCACGTAG+AGG 0.676462 5.2:-12977981 MS.gene64753:CDS
GACGGAGGATTCGCATACGG+TGG 0.678471 5.2:-12977781 MS.gene64753:CDS
TGATTATGTTGTGGCGACGG+AGG 0.681152 5.2:-12977796 MS.gene64753:CDS
ACGAAATTCTCTCCTCTACG+TGG 0.681620 5.2:+12977969 None:intergenic
ATTAATGCGACTTTCCGCAG+TGG 0.684074 5.2:+12978746 None:intergenic
CGACGTGAGACTGTGCGGCG+AGG 0.686982 5.2:+12978486 None:intergenic
GGTTGTAAACTTCGTCAGGG+AGG 0.692198 5.2:+12978507 None:intergenic
GAGGCGGCGTAAGGAGACCG+AGG 0.699155 5.2:+12978278 None:intergenic
CGCACCGGTAGCAACAACAT+CGG 0.701461 5.2:+12978433 None:intergenic
AAGGGCTGATGATTATGTTG+TGG 0.716548 5.2:-12977805 MS.gene64753:CDS
GAAGTCGTTTCAGACTGCGG+CGG 0.732622 5.2:+12978308 None:intergenic
GCGACACCACTCCTCCACTG+CGG 0.736296 5.2:-12978760 MS.gene64753:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATGCATAAATTAATGAAA+TGG - chr5.2:12978137-12978156 MS.gene64753:intron 15.0%
!!! CTAACTTTTACATCTATAAT+AGG - chr5.2:12978182-12978201 MS.gene64753:intron 20.0%
! TAATGAAATGGAAATAGCAA+CGG - chr5.2:12978149-12978168 MS.gene64753:intron 25.0%
! TTAAGATTATCAACTTCAGT+AGG + chr5.2:12978661-12978680 None:intergenic 25.0%
!! TATGGAATTTTTGCTTGTAA+CGG - chr5.2:12978322-12978341 MS.gene64753:CDS 25.0%
!!! ACTGAAGTTGATAATCTTAA+AGG - chr5.2:12978661-12978680 MS.gene64753:CDS 25.0%
CAAAAATTCCATATGCTTCA+CGG + chr5.2:12978315-12978334 None:intergenic 30.0%
TTATGTTGGATTGAATTGGA+AGG - chr5.2:12978603-12978622 MS.gene64753:CDS 30.0%
TTGGTTATGTTGGATTGAAT+TGG - chr5.2:12978599-12978618 MS.gene64753:CDS 30.0%
! CTAACTTTTACATCTCGAAT+AGG - chr5.2:12978275-12978294 MS.gene64753:CDS 30.0%
!! GGTTGATAATGATATTGAGA+TGG - chr5.2:12978756-12978775 MS.gene64753:CDS 30.0%
AGCTCTTCAAAACTAACCTT+AGG + chr5.2:12978724-12978743 None:intergenic 35.0%
AGGAAGATTACTAGAGTTGT+TGG - chr5.2:12978394-12978413 MS.gene64753:CDS 35.0%
AGTAAAGCCAAGAAAGTTCT+TGG - chr5.2:12978691-12978710 MS.gene64753:CDS 35.0%
GTTGTTGGAAGAATCAAGAT+CGG - chr5.2:12978409-12978428 MS.gene64753:CDS 35.0%
TGATATTGAGATGGCTAAGA+AGG - chr5.2:12978765-12978784 MS.gene64753:CDS 35.0%
TTGTTGGAAGAATCAAGATC+GGG - chr5.2:12978410-12978429 MS.gene64753:CDS 35.0%
TTTCCAACCAAGAACTTTCT+TGG + chr5.2:12978701-12978720 None:intergenic 35.0%
!! CTTCAGAGTAAGTTGTTTCT+TGG - chr5.2:12978433-12978452 MS.gene64753:CDS 35.0%
!! GAGTTTTTGGAAGTTGCTTT+TGG - chr5.2:12978580-12978599 MS.gene64753:CDS 35.0%
!! TGAAGAGCTTGTTAGAATGA+TGG - chr5.2:12978735-12978754 MS.gene64753:CDS 35.0%
!!! GAAGTTGCTTTTGGTTATGT+TGG - chr5.2:12978589-12978608 MS.gene64753:CDS 35.0%
AAACGACTTCGTTTCATCCT+CGG - chr5.2:12978041-12978060 MS.gene64753:CDS 40.0%
AAGCCAAGAAAGTTCTTGGT+TGG - chr5.2:12978695-12978714 MS.gene64753:CDS 40.0%
AAGGGCTGATGATTATGTTG+TGG - chr5.2:12978531-12978550 MS.gene64753:CDS 40.0%
AATCATGAATCACCACGTAG+AGG - chr5.2:12978355-12978374 MS.gene64753:CDS 40.0%
ACAGAATTCCTCCTCAACAA+AGG - chr5.2:12977647-12977666 MS.gene64753:CDS 40.0%
AGAAAGTCCTTGTTGATGCT+GGG - chr5.2:12978788-12978807 MS.gene64753:CDS 40.0%
AGTTCTTGGTTGGAAACCTA+AGG - chr5.2:12978705-12978724 MS.gene64753:CDS 40.0%
CAACAGGAAGATCTCACATA+CGG - chr5.2:12977963-12977982 MS.gene64753:CDS 40.0%
CATCGCTTCAACATAATCAC+CGG + chr5.2:12978491-12978510 None:intergenic 40.0%
GAGGAGAGAATTTCGTTACA+AGG - chr5.2:12978374-12978393 MS.gene64753:CDS 40.0%
GTGAATTACCGTGAAGCATA+TGG - chr5.2:12978304-12978323 MS.gene64753:CDS 40.0%
GTGATTATGTTGAAGCGATG+TGG - chr5.2:12978491-12978510 MS.gene64753:CDS 40.0%
GTGTGTTGAAGTTTGACGAA+CGG + chr5.2:12977697-12977716 None:intergenic 40.0%
GTTGATGCTACAACAGGAAA+GGG - chr5.2:12978513-12978532 MS.gene64753:CDS 40.0%
TGATGATTATGTTGTGGCGA+CGG - chr5.2:12978537-12978556 MS.gene64753:CDS 40.0%
TGTTGAGGAGGAATTCTGTT+AGG + chr5.2:12977646-12977665 None:intergenic 40.0%
! CTGTTGCATCGATATGAGAT+CGG + chr5.2:12977949-12977968 None:intergenic 40.0%
!! GCTGGATCTTCTGAAATGTT+TGG - chr5.2:12977995-12978014 MS.gene64753:CDS 40.0%
AACGAAGTCGTTTCAGACTG+CGG + chr5.2:12978034-12978053 None:intergenic 45.0%
ACAACATCGGCAGTGTAATC+AGG + chr5.2:12977893-12977912 None:intergenic 45.0%
ACGAAATTCTCTCCTCTACG+TGG + chr5.2:12978370-12978389 None:intergenic 45.0%
AGTTTGACGAACGGCGAATT+AGG + chr5.2:12977688-12977707 None:intergenic 45.0%
ATTAATGCGACTTTCCGCAG+TGG + chr5.2:12977593-12977612 None:intergenic 45.0%
CAATGTACCCAGCATCAACA+AGG + chr5.2:12978798-12978817 None:intergenic 45.0%
CACATACGGTACTATCAAGC+TGG - chr5.2:12977977-12977996 MS.gene64753:CDS 45.0%
CGAGGTTGTAAACTTCGTCA+GGG + chr5.2:12977835-12977854 None:intergenic 45.0%
CGATCTCATATCGATGCAAC+AGG - chr5.2:12977947-12977966 MS.gene64753:CDS 45.0%
CGGAAAGTCGCATTAATCAC+CGG - chr5.2:12977596-12977615 MS.gene64753:CDS 45.0%
CTGTTAGGTATGATCCGTCT+TGG + chr5.2:12977631-12977650 None:intergenic 45.0%
GAGAAAGTCCTTGTTGATGC+TGG - chr5.2:12978787-12978806 MS.gene64753:CDS 45.0%
GCATTAATCACCGGAATCAC+CGG - chr5.2:12977605-12977624 MS.gene64753:CDS 45.0%
! GATGTGGTTGATGCTACAAC+AGG - chr5.2:12978507-12978526 MS.gene64753:CDS 45.0%
! GCATACGGTGGAAGAGTTTT+TGG - chr5.2:12978567-12978586 MS.gene64753:CDS 45.0%
! GGTTGATGCTACAACAGGAA+AGG - chr5.2:12978512-12978531 MS.gene64753:CDS 45.0%
! TGCATCGATATGAGATCGGA+CGG + chr5.2:12977945-12977964 None:intergenic 45.0%
AACAAAGGCTACTCCGTCCA+CGG - chr5.2:12977662-12977681 MS.gene64753:CDS 50.0%
ACCTAAGTGCGTCGAGAGAT+TGG - chr5.2:12978458-12978477 MS.gene64753:CDS 50.0%
ACTGCCGATGTTGTTGCTAC+CGG - chr5.2:12977899-12977918 MS.gene64753:CDS 50.0%
ATCGGACGGCTTCAAGAAGA+CGG + chr5.2:12977931-12977950 None:intergenic 50.0%
CCAACACCGCCAACAACAAA+CGG - chr5.2:12977554-12977573 MS.gene64753:CDS 50.0%
CCTAAGTGCGTCGAGAGATT+GGG - chr5.2:12978459-12978478 MS.gene64753:CDS 50.0%
CTAAGTGCGTCGAGAGATTG+GGG - chr5.2:12978460-12978479 MS.gene64753:CDS 50.0%
GCGAGGTTGTAAACTTCGTC+AGG + chr5.2:12977836-12977855 None:intergenic 50.0%
GGTTGTAAACTTCGTCAGGG+AGG + chr5.2:12977832-12977851 None:intergenic 50.0%
TAGGTATGATCCGTCTTGGC+CGG + chr5.2:12977627-12977646 None:intergenic 50.0%
TCGAGAGATTGGGGATTTGC+CGG - chr5.2:12978469-12978488 MS.gene64753:CDS 50.0%
TGATTATGTTGTGGCGACGG+AGG - chr5.2:12978540-12978559 MS.gene64753:CDS 50.0%
! TGAGCTTTGTGAGTGTCGTG+TGG + chr5.2:12977746-12977765 None:intergenic 50.0%
!! AGAGGAAGCGTCAGAGAGAT+CGG + chr5.2:12977786-12977805 None:intergenic 50.0%
!! CGTTTGTTGTTGGCGGTGTT+GGG + chr5.2:12977556-12977575 None:intergenic 50.0%
!! GTTTGTTGTTGGCGGTGTTG+GGG + chr5.2:12977555-12977574 None:intergenic 50.0%
AATGCGACTTTCCGCAGTGG+AGG + chr5.2:12977590-12977609 None:intergenic 55.0%
CCCAATCTCTCGACGCACTT+AGG + chr5.2:12978462-12978481 None:intergenic 55.0%
CGGAGTAGCCTTTGTTGAGG+AGG + chr5.2:12977658-12977677 None:intergenic 55.0%
GAAGTCGTTTCAGACTGCGG+CGG + chr5.2:12978031-12978050 None:intergenic 55.0%
GGACGGAGTAGCCTTTGTTG+AGG + chr5.2:12977661-12977680 None:intergenic 55.0%
GTACCAATGCGCAGCGACTT+TGG + chr5.2:12978083-12978102 None:intergenic 55.0%
TTCAAGAAGACGGAGCGCAC+CGG + chr5.2:12977921-12977940 None:intergenic 55.0%
! CGCACCGGTAGCAACAACAT+CGG + chr5.2:12977906-12977925 None:intergenic 55.0%
!! CCGTTTGTTGTTGGCGGTGT+TGG + chr5.2:12977557-12977576 None:intergenic 55.0%
!! GTGGTGTCGCCGTTTGTTGT+TGG + chr5.2:12977566-12977585 None:intergenic 55.0%
!! GTGTCGCCGTTTGTTGTTGG+CGG + chr5.2:12977563-12977582 None:intergenic 55.0%
!! GTTGGCGGTGTTGGGGTTTT+GGG + chr5.2:12977548-12977567 None:intergenic 55.0%
!! TGTTGGCGGTGTTGGGGTTT+TGG + chr5.2:12977549-12977568 None:intergenic 55.0%
AACCGCGACGTGAGACTGTG+CGG + chr5.2:12977858-12977877 None:intergenic 60.0%
ACCGGAATCACCGGCCAAGA+CGG - chr5.2:12977614-12977633 MS.gene64753:CDS 60.0%
ACGAACGGCGAATTAGGCCG+TGG + chr5.2:12977682-12977701 None:intergenic 60.0%
ACGGCGAATTAGGCCGTGGA+CGG + chr5.2:12977678-12977697 None:intergenic 60.0%
CCAATGCGCAGCGACTTTGG+AGG + chr5.2:12978080-12978099 None:intergenic 60.0%
CCTCCAAAGTCGCTGCGCAT+TGG - chr5.2:12978077-12978096 MS.gene64753:intron 60.0%
GACGGAGGATTCGCATACGG+TGG - chr5.2:12978555-12978574 MS.gene64753:CDS 60.0%
GACTTTCCGCAGTGGAGGAG+TGG + chr5.2:12977585-12977604 None:intergenic 60.0%
GGCGACGGAGGATTCGCATA+CGG - chr5.2:12978552-12978571 MS.gene64753:CDS 60.0%
TCCGTCTTGGCCGGTGATTC+CGG + chr5.2:12977618-12977637 None:intergenic 60.0%
! ATGCGCAGCGACTTTGGAGG+CGG + chr5.2:12978077-12978096 None:intergenic 60.0%
! GGATAGTGTCGAGCCAGCGA+CGG + chr5.2:12977811-12977830 None:intergenic 60.0%
ACGCTTCCTCTCTCCGTCGC+TGG - chr5.2:12977795-12977814 MS.gene64753:CDS 65.0%
GCGACACCACTCCTCCACTG+CGG - chr5.2:12977576-12977595 MS.gene64753:CDS 65.0%
! GCGACTTTGGAGGCGGCGTA+AGG + chr5.2:12978070-12978089 None:intergenic 65.0%
CGACGTGAGACTGTGCGGCG+AGG + chr5.2:12977853-12977872 None:intergenic 70.0%
CGCCGCACAGTCTCACGTCG+CGG - chr5.2:12977853-12977872 MS.gene64753:CDS 70.0%
GAGGCGGCGTAAGGAGACCG+AGG + chr5.2:12978061-12978080 None:intergenic 70.0%
GTCGAGCCAGCGACGGAGAG+AGG + chr5.2:12977804-12977823 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 12977530 12978828 12977530 ID=MS.gene64753
chr5.2 mRNA 12977530 12978828 12977530 ID=MS.gene64753.t1;Parent=MS.gene64753
chr5.2 exon 12978260 12978828 12978260 ID=MS.gene64753.t1.exon1;Parent=MS.gene64753.t1
chr5.2 CDS 12978260 12978828 12978260 ID=cds.MS.gene64753.t1;Parent=MS.gene64753.t1
chr5.2 exon 12977530 12978061 12977530 ID=MS.gene64753.t1.exon2;Parent=MS.gene64753.t1
chr5.2 CDS 12977530 12978061 12977530 ID=cds.MS.gene64753.t1;Parent=MS.gene64753.t1
Gene Sequence

>MS.gene64753

ATGGCAGACTCATCACCCAAAACCCCAACACCGCCAACAACAAACGGCGACACCACTCCTCCACTGCGGAAAGTCGCATTAATCACCGGAATCACCGGCCAAGACGGATCATACCTAACAGAATTCCTCCTCAACAAAGGCTACTCCGTCCACGGCCTAATTCGCCGTTCGTCAAACTTCAACACACAACGAATTGATCACATCTACGTCGATCCACACGACACTCACAAAGCTCATATGAAGCTTCACTACGCCGATCTCTCTGACGCTTCCTCTCTCCGTCGCTGGCTCGACACTATCCTCCCTGACGAAGTTTACAACCTCGCCGCACAGTCTCACGTCGCGGTTTCGTTTGAGATTCCTGATTACACTGCCGATGTTGTTGCTACCGGTGCGCTCCGTCTTCTTGAAGCCGTCCGATCTCATATCGATGCAACAGGAAGATCTCACATACGGTACTATCAAGCTGGATCTTCTGAAATGTTTGGATCAACTCTGCCGCCGCAGTCTGAAACGACTTCGTTTCATCCTCGGTCTCCTTACGCCGCCTCCAAAGTCGCTGCGCATTGGTACTATTACAATTCTTTATATAACTCATAAATAAGATAAATGCATAAATTAATGAAATGGAAATAGCAACGGATATCGATAACTAACTTTTACATCTATAATAGGTACGTTGTAAAAATAACATAACTAACTTTTACATCTCTAATACTGTGAAAAACAACGATACTGATATGAACTAACTTTTACATCTCGAATAGGTACACTGTGAATTACCGTGAAGCATATGGAATTTTTGCTTGTAACGGAATTCTCTTCAATCATGAATCACCACGTAGAGGAGAGAATTTCGTTACAAGGAAGATTACTAGAGTTGTTGGAAGAATCAAGATCGGGCTTCAGAGTAAGTTGTTTCTTGGAAACCTAAGTGCGTCGAGAGATTGGGGATTTGCCGGTGATTATGTTGAAGCGATGTGGTTGATGCTACAACAGGAAAGGGCTGATGATTATGTTGTGGCGACGGAGGATTCGCATACGGTGGAAGAGTTTTTGGAAGTTGCTTTTGGTTATGTTGGATTGAATTGGAAGGATCATGTTGTGATTGATAAGAGATACTTTCGTCCTACTGAAGTTGATAATCTTAAAGGTGATGCTAGTAAAGCCAAGAAAGTTCTTGGTTGGAAACCTAAGGTTAGTTTTGAAGAGCTTGTTAGAATGATGGTTGATAATGATATTGAGATGGCTAAGAAGGAGAAAGTCCTTGTTGATGCTGGGTACATTGATGCTCAGTAA
Protein sequence

>MS.gene64753.t1

MADSSPKTPTPPTTNGDTTPPLRKVALITGITGQDGSYLTEFLLNKGYSVHGLIRRSSNFNTQRIDHIYVDPHDTHKAHMKLHYADLSDASSLRRWLDTILPDEVYNLAAQSHVAVSFEIPDYTADVVATGALRLLEAVRSHIDATGRSHIRYYQAGSSEMFGSTLPPQSETTSFHPRSPYAASKVAAHWYTVNYREAYGIFACNGILFNHESPRRGENFVTRKITRVVGRIKIGLQSKLFLGNLSASRDWGFAGDYVEAMWLMLQQERADDYVVATEDSHTVEEFLEVAFGYVGLNWKDHVVIDKRYFRPTEVDNLKGDASKAKKVLGWKPKVSFEELVRMMVDNDIEMAKKEKVLVDAGYIDAQ