AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene65274

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65274.t1 MTR_4g461080 98.425 127 2 0 13 139 1 127 2.80e-89 262
MS.gene65274.t1 MTR_4g461080 93.333 45 3 0 135 179 233 277 6.70e-24 95.1
MS.gene65274.t1 MTR_2g093220 62.577 163 52 4 19 176 18 176 1.28e-56 180
MS.gene65274.t1 MTR_3g077750 52.778 144 44 5 2 137 28 155 1.46e-35 127
MS.gene65274.t1 MTR_3g091820 76.471 68 16 0 30 97 32 99 1.28e-34 124
MS.gene65274.t1 MTR_1g077600 56.122 98 39 1 3 96 1 98 2.01e-34 122
MS.gene65274.t1 MTR_1g077600 56.122 98 39 1 3 96 1 98 2.20e-34 122
MS.gene65274.t1 MTR_4g063780 66.304 92 26 2 25 111 58 149 4.16e-34 123
MS.gene65274.t1 MTR_8g015840 70.270 74 22 0 29 102 8 81 7.80e-33 117
MS.gene65274.t1 MTR_2g096740 60.000 85 34 0 12 96 33 117 1.01e-32 118
MS.gene65274.t1 MTR_2g096740 60.000 85 34 0 12 96 48 132 1.39e-32 118
MS.gene65274.t1 MTR_3g090430 70.130 77 23 0 23 99 33 109 1.79e-32 119
MS.gene65274.t1 MTR_4g022370 66.250 80 26 1 17 95 57 136 2.83e-32 119
MS.gene65274.t1 MTR_4g088580 60.204 98 29 2 42 129 56 153 5.40e-32 118
MS.gene65274.t1 MTR_8g027295 66.234 77 24 1 23 99 8 82 7.40e-32 115
MS.gene65274.t1 MTR_1g056810 64.368 87 27 2 43 125 14 100 9.05e-32 115
MS.gene65274.t1 MTR_7g059400 68.831 77 23 1 20 95 54 130 1.78e-31 116
MS.gene65274.t1 MTR_8g479350 82.143 56 10 0 39 94 66 121 2.52e-31 115
MS.gene65274.t1 MTR_2g014060 85.965 57 6 1 36 92 22 76 3.70e-31 115
MS.gene65274.t1 MTR_8g068210 58.333 84 35 0 30 113 21 104 4.26e-31 115
MS.gene65274.t1 MTR_4g109980 86.792 53 7 0 39 91 38 90 5.03e-30 112
MS.gene65274.t1 MTR_7g024670 63.095 84 30 1 26 108 62 145 8.07e-30 112
MS.gene65274.t1 MTR_8g079060 70.588 68 18 1 32 97 18 85 9.56e-30 109
MS.gene65274.t1 MTR_5g031440 80.702 57 10 1 35 91 38 93 1.04e-29 111
MS.gene65274.t1 MTR_2g014170 90.000 50 5 0 40 89 44 93 1.18e-28 108
MS.gene65274.t1 MTR_2g013370 60.465 86 31 2 10 95 17 99 2.72e-28 106
MS.gene65274.t1 MTR_2g059540 88.000 50 6 0 40 89 30 79 4.37e-28 104
MS.gene65274.t1 MTR_4g089095 51.852 108 34 3 1 95 1 103 8.23e-28 105
MS.gene65274.t1 MTR_4g089095 80.702 57 10 1 39 95 14 69 1.08e-27 104
MS.gene65274.t1 MTR_3g435480 66.197 71 23 1 29 99 125 194 1.12e-26 105
MS.gene65274.t1 MTR_7g010950 67.143 70 22 1 29 98 124 192 1.34e-26 105
MS.gene65274.t1 MTR_6g027460 51.042 96 45 2 4 98 59 153 1.42e-26 103
MS.gene65274.t1 MTR_5g041420 59.740 77 30 1 29 105 127 202 4.36e-26 101
MS.gene65274.t1 MTR_6g027450 46.809 94 49 1 5 98 54 146 5.54e-26 101
MS.gene65274.t1 MTR_6g012450 52.174 92 43 1 7 98 103 193 2.74e-25 101
MS.gene65274.t1 MTR_7g086780 59.722 72 28 1 27 98 86 156 6.66e-25 100
MS.gene65274.t1 MTR_4g082060 54.321 81 33 1 19 99 104 180 1.14e-24 99.8
MS.gene65274.t1 MTR_8g044220 47.059 102 40 3 29 128 107 196 2.78e-24 98.2
MS.gene65274.t1 MTR_2g016030 67.857 56 18 0 39 94 45 100 1.53e-23 91.3
MS.gene65274.t1 MTR_5g041380 59.211 76 27 1 25 100 115 186 1.01e-22 93.6
MS.gene65274.t1 MTR_5g041400 59.211 76 27 1 25 100 107 178 1.33e-22 93.2
MS.gene65274.t1 MTR_5g041530 58.571 70 28 1 25 94 125 193 1.26e-21 90.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65274.t1 AT5G60200 58.197 122 45 3 25 141 40 160 9.79e-42 140
MS.gene65274.t1 AT2G28510 54.967 151 46 6 10 138 4 154 1.52e-40 139
MS.gene65274.t1 AT3G45610 47.799 159 76 5 3 158 1 155 4.43e-39 134
MS.gene65274.t1 AT1G51700 63.158 95 31 2 21 114 16 107 1.18e-35 123
MS.gene65274.t1 AT3G55370 71.429 77 21 1 25 100 104 180 1.02e-33 122
MS.gene65274.t1 AT3G55370 71.429 77 21 1 25 100 59 135 1.44e-33 121
MS.gene65274.t1 AT3G55370 71.429 77 21 1 25 100 59 135 1.98e-33 122
MS.gene65274.t1 AT5G02460 73.611 72 18 1 25 95 78 149 9.36e-33 120
MS.gene65274.t1 AT4G24060 72.727 66 18 0 30 95 42 107 2.00e-32 119
MS.gene65274.t1 AT1G21340 65.116 86 18 1 40 113 37 122 2.51e-32 116
MS.gene65274.t1 AT1G07640 65.823 79 26 1 18 95 53 131 2.78e-32 118
MS.gene65274.t1 AT1G07640 65.823 79 26 1 18 95 61 139 3.76e-32 118
MS.gene65274.t1 AT3G52440 59.574 94 33 1 43 136 27 115 4.24e-32 115
MS.gene65274.t1 AT5G60850 72.000 75 19 1 39 111 49 123 4.86e-32 117
MS.gene65274.t1 AT3G52440 59.574 94 33 1 43 136 46 134 5.27e-32 116
MS.gene65274.t1 AT2G37590 67.123 73 23 1 25 96 72 144 6.75e-32 117
MS.gene65274.t1 AT1G07640 69.444 72 21 1 25 95 4 75 1.00e-31 115
MS.gene65274.t1 AT2G28810 69.565 69 20 1 24 92 61 128 1.52e-31 116
MS.gene65274.t1 AT2G28810 69.565 69 20 1 24 92 78 145 2.62e-31 115
MS.gene65274.t1 AT3G61850 70.833 72 20 1 27 98 49 119 2.69e-31 114
MS.gene65274.t1 AT3G61850 70.833 72 20 1 27 98 49 119 2.69e-31 114
MS.gene65274.t1 AT3G61850 70.833 72 20 1 27 98 61 131 3.50e-31 114
MS.gene65274.t1 AT3G61850 70.833 72 20 1 27 98 49 119 3.83e-31 115
MS.gene65274.t1 AT5G62940 77.049 61 14 0 31 91 63 123 3.87e-31 116
MS.gene65274.t1 AT3G61850 70.833 72 20 1 27 98 61 131 4.39e-31 115
MS.gene65274.t1 AT5G65590 80.328 61 12 0 41 101 41 101 6.99e-31 114
MS.gene65274.t1 AT1G28310 87.273 55 7 0 41 95 27 81 1.48e-30 113
MS.gene65274.t1 AT1G28310 87.273 55 7 0 41 95 51 105 2.34e-30 113
MS.gene65274.t1 AT3G21270 90.566 53 5 0 41 93 29 81 8.59e-30 108
MS.gene65274.t1 AT4G38000 72.727 66 17 1 30 94 30 95 2.14e-29 108
MS.gene65274.t1 AT3G50410 72.308 65 18 0 33 97 22 86 3.03e-29 108
MS.gene65274.t1 AT4G21050 63.158 76 28 0 38 113 21 96 3.85e-29 107
MS.gene65274.t1 AT5G66940 61.905 84 25 2 37 113 28 111 8.90e-29 106
MS.gene65274.t1 AT4G00940 80.702 57 11 0 39 95 66 122 1.22e-28 108
MS.gene65274.t1 AT4G00940 80.702 57 11 0 39 95 66 122 1.22e-28 108
MS.gene65274.t1 AT4G00940 80.702 57 11 0 39 95 66 122 1.22e-28 108
MS.gene65274.t1 AT1G64620 72.131 61 17 0 30 90 38 98 2.88e-28 108
MS.gene65274.t1 AT2G46590 63.158 76 24 1 13 88 44 115 5.37e-28 107
MS.gene65274.t1 AT2G46590 63.158 76 24 1 13 88 56 127 6.47e-28 107
MS.gene65274.t1 AT1G47655 75.439 57 14 0 33 89 21 77 1.40e-26 100
MS.gene65274.t1 AT5G62430 47.959 98 45 2 3 100 22 113 1.85e-26 102
MS.gene65274.t1 AT3G47500 52.632 95 41 1 4 98 77 167 3.01e-26 103
MS.gene65274.t1 AT5G39660 61.333 75 25 1 24 98 125 195 4.43e-25 100
MS.gene65274.t1 AT5G39660 61.333 75 25 1 24 98 125 195 4.43e-25 100
MS.gene65274.t1 AT5G39660 61.333 75 25 1 24 98 125 195 4.43e-25 100
MS.gene65274.t1 AT1G69570 53.488 86 36 1 9 94 104 185 8.22e-25 99.4
MS.gene65274.t1 AT4G21080 56.579 76 24 1 43 109 27 102 9.11e-25 97.1
MS.gene65274.t1 AT1G26790 55.263 76 33 1 20 94 89 164 2.45e-24 97.8
MS.gene65274.t1 AT4G21040 60.274 73 29 0 22 94 6 78 3.48e-24 95.1
MS.gene65274.t1 AT2G34140 66.071 56 19 0 39 94 56 111 1.77e-23 91.7
MS.gene65274.t1 AT1G29160 66.071 56 19 0 39 94 60 115 4.22e-23 90.9
MS.gene65274.t1 AT4G21030 53.968 63 29 0 43 105 23 85 1.62e-15 71.2

Find 50 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGAAACAAGTGAGTGGATT+TGG 0.231178 4.3:-41397328 None:intergenic
GCTTCTGGTTGAGGAGGTTT+TGG 0.248273 4.3:-41397088 None:intergenic
GCAAGTCTTGTAGGAGATAT+TGG 0.255429 4.3:+41397194 MS.gene65274:CDS
GAATGGTCTCACTTCATCTT+GGG 0.274434 4.3:+41397807 MS.gene65274:CDS
GATATTTCTGCAAGTCTTGT+AGG 0.294030 4.3:+41397185 MS.gene65274:CDS
GGAATGGTCTCACTTCATCT+TGG 0.324184 4.3:+41397806 MS.gene65274:CDS
ATTGTTGTAGTAACAAAATT+TGG 0.325870 4.3:-41397143 None:intergenic
TTTCCATGGCAGTTCAATAA+TGG 0.330980 4.3:+41397739 MS.gene65274:CDS
GGAAACAAGTGAGTGGATTT+GGG 0.349121 4.3:-41397327 None:intergenic
ACGAGACGGTGTCTACATTA+GGG 0.358019 4.3:-41397851 None:intergenic
ATGGCTGAAATGGATCATTT+AGG 0.361202 4.3:+41397772 MS.gene65274:CDS
AGACACAAATATGGCTGAAA+TGG 0.367887 4.3:+41397762 MS.gene65274:CDS
CACGAGACGGTGTCTACATT+AGG 0.377042 4.3:-41397852 None:intergenic
CAATAATGGAGACACAAATA+TGG 0.378026 4.3:+41397753 MS.gene65274:CDS
CAAGACTTGCAGAAATATCT+TGG 0.387546 4.3:-41397181 None:intergenic
AGGAATGTTCCTGTTGGTGG+GGG 0.412807 4.3:+41397235 MS.gene65274:CDS
TTCTTCTAGTGGACAACAAC+AGG 0.413714 4.3:+41396348 MS.gene65274:CDS
TGTGAGATCATTGGAATCAT+AGG 0.425382 4.3:-41397359 None:intergenic
GGATTTGGGTTGGTTTGAAA+TGG 0.429582 4.3:-41397313 None:intergenic
AGCATTAGCTAGGCTTCAAA+AGG 0.442460 4.3:+41397384 MS.gene65274:CDS
GCAAATGTCTAACCAGTCAT+TGG 0.467897 4.3:+41397024 MS.gene65274:intron
AAGAAGTGTTTGTATGGGGA+TGG 0.488483 4.3:+41396324 None:intergenic
ACAAGTGAGTGGATTTGGGT+TGG 0.491105 4.3:-41397323 None:intergenic
TAAGGAATGTTCCTGTTGGT+GGG 0.496140 4.3:+41397233 MS.gene65274:CDS
AATGGTCTCACTTCATCTTG+GGG 0.497543 4.3:+41397808 MS.gene65274:CDS
TGTAGGAGATATTGGACTAA+AGG 0.499003 4.3:+41397202 MS.gene65274:CDS
GTGTTGTTTGAGTCACATCT+TGG 0.500769 4.3:-41397121 None:intergenic
ATGGATCATTTAGGAAGAGC+TGG 0.502060 4.3:+41397781 MS.gene65274:CDS
TAATGCTAGTGTGAGATCAT+TGG 0.503293 4.3:-41397368 None:intergenic
TTGGAATCATAGGACATTGA+AGG 0.508739 4.3:-41397349 None:intergenic
TTAGGAAGAGCTGGTTGGAA+TGG 0.509850 4.3:+41397790 MS.gene65274:CDS
CTAAGGAATGTTCCTGTTGG+TGG 0.517333 4.3:+41397232 MS.gene65274:CDS
ATGGGGATGGATTCTTCTAG+TGG 0.553754 4.3:+41396337 MS.gene65274:CDS
TTTAGAGCTTCTGGTTGAGG+AGG 0.557206 4.3:-41397094 None:intergenic
AAGGAATGTTCCTGTTGGTG+GGG 0.558824 4.3:+41397234 MS.gene65274:CDS
TTAACATGCTCTCCAATGAC+TGG 0.564124 4.3:-41397036 None:intergenic
GGACTAAAGGTGGTACACTA+AGG 0.568077 4.3:+41397215 MS.gene65274:CDS
ATCATTTAGGAAGAGCTGGT+TGG 0.573027 4.3:+41397785 MS.gene65274:CDS
CGAGACGGTGTCTACATTAG+GGG 0.579103 4.3:-41397850 None:intergenic
TGGAATCATAGGACATTGAA+GGG 0.583945 4.3:-41397348 None:intergenic
GGAATGTTCCTGTTGGTGGG+GGG 0.588003 4.3:+41397236 MS.gene65274:CDS
ATCTCACACTAGCATTAGCT+AGG 0.588061 4.3:+41397374 MS.gene65274:CDS
CATCATCAGCTTCATCAAAG+AGG 0.595811 4.3:+41397278 MS.gene65274:CDS
ATGGTCTCACTTCATCTTGG+GGG 0.616298 4.3:+41397809 MS.gene65274:CDS
TCTCCATTATTGAACTGCCA+TGG 0.629912 4.3:-41397742 None:intergenic
GCATTAGCTAGGCTTCAAAA+GGG 0.630153 4.3:+41397385 MS.gene65274:CDS
GAGACGGTGTCTACATTAGG+GGG 0.640287 4.3:-41397849 None:intergenic
AGGAGATATTGGACTAAAGG+TGG 0.646558 4.3:+41397205 MS.gene65274:CDS
ACACTAAGGAATGTTCCTGT+TGG 0.660187 4.3:+41397229 MS.gene65274:CDS
ATTGAAGGGAAACAAGTGAG+TGG 0.662440 4.3:-41397334 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATACATAATAAAATTGTAGA+GGG - chr4.3:41396586-41396605 None:intergenic 15.0%
!! CAAAAATTAAGGAAAATAAT+AGG - chr4.3:41396512-41396531 None:intergenic 15.0%
!! TATACATAATAAAATTGTAG+AGG - chr4.3:41396587-41396606 None:intergenic 15.0%
!! ATTGTTGTAGTAACAAAATT+TGG - chr4.3:41397146-41397165 None:intergenic 20.0%
!! CAAAGAAGAAACAAAAATTA+AGG - chr4.3:41396523-41396542 None:intergenic 20.0%
!! TCATGTAATATTTATTAACG+TGG + chr4.3:41396414-41396433 MS.gene65274:intron 20.0%
!! TTCCATCAAATATTAATTCT+AGG - chr4.3:41396660-41396679 None:intergenic 20.0%
!!! ACTAATTTTATTTCATCCAA+GGG - chr4.3:41396553-41396572 None:intergenic 20.0%
!!! TACTAATTTTATTTCATCCA+AGG - chr4.3:41396554-41396573 None:intergenic 20.0%
!!! TTTACTTTATTTGATCTAGA+GGG - chr4.3:41396737-41396756 None:intergenic 20.0%
!!! TTTTACTTTATTTGATCTAG+AGG - chr4.3:41396738-41396757 None:intergenic 20.0%
! AACAAGAAATGTGTAATGTT+AGG + chr4.3:41397686-41397705 MS.gene65274:intron 25.0%
! ACAAGAAATGTGTAATGTTA+GGG + chr4.3:41397687-41397706 MS.gene65274:intron 25.0%
! ATATTTGATGGAATAAAGTC+AGG + chr4.3:41396667-41396686 MS.gene65274:intron 25.0%
! CTTAACATTATGTTTGAATG+TGG + chr4.3:41396821-41396840 MS.gene65274:intron 25.0%
! GTAGTACTAAAAATTCATGA+AGG - chr4.3:41396469-41396488 None:intergenic 25.0%
! TAGGGAACAAAATGAAAATA+GGG + chr4.3:41397705-41397724 MS.gene65274:intron 25.0%
! TATTTGATGGAATAAAGTCA+GGG + chr4.3:41396668-41396687 MS.gene65274:intron 25.0%
! TCGCAAAAATTTGTACTATT+TGG + chr4.3:41396962-41396981 MS.gene65274:intron 25.0%
! TGTATATACTATACTACTCA+TGG + chr4.3:41396601-41396620 MS.gene65274:intron 25.0%
! TTAGGGAACAAAATGAAAAT+AGG + chr4.3:41397704-41397723 MS.gene65274:intron 25.0%
!! GAACATGATTTTTCCATTTT+AGG + chr4.3:41397427-41397446 MS.gene65274:intron 25.0%
!! TATTAATGGATGTACATGTT+GGG + chr4.3:41396767-41396786 MS.gene65274:intron 25.0%
!! TTACTTTATTTGATCTAGAG+GGG - chr4.3:41396736-41396755 None:intergenic 25.0%
!! TTATTAATGGATGTACATGT+TGG + chr4.3:41396766-41396785 MS.gene65274:intron 25.0%
!!! CATGAATTTTTAGTACTACT+TGG + chr4.3:41396470-41396489 MS.gene65274:intron 25.0%
AAATTCATGAAGGACAAAGA+TGG - chr4.3:41396459-41396478 None:intergenic 30.0%
ACACAACACAACAAATTTAG+TGG - chr4.3:41396997-41397016 None:intergenic 30.0%
ATGAACCATATGTATATAGC+TGG + chr4.3:41396632-41396651 MS.gene65274:intron 30.0%
ATGCTCATTTCATGATCATA+AGG - chr4.3:41397631-41397650 None:intergenic 30.0%
ATTGTTAAGAATACCACATG+AGG - chr4.3:41397467-41397486 None:intergenic 30.0%
GCTATATACATATGGTTCAT+AGG - chr4.3:41396632-41396651 None:intergenic 30.0%
GGCCTAGAATTAATATTTGA+TGG + chr4.3:41396655-41396674 MS.gene65274:intron 30.0%
TGAACCATATGTATATAGCT+GGG + chr4.3:41396633-41396652 MS.gene65274:intron 30.0%
TGTGGTATTCTTAACAATCA+TGG + chr4.3:41397469-41397488 MS.gene65274:intron 30.0%
! CAATAATGGAGACACAAATA+TGG + chr4.3:41397753-41397772 MS.gene65274:CDS 30.0%
!! AATGAAAATAGGGTGTTGTT+AGG + chr4.3:41397715-41397734 MS.gene65274:intron 30.0%
AAAGAATCCTTGGTTGTTTG+AGG - chr4.3:41397506-41397525 None:intergenic 35.0%
AAAGATTCTGCACATTGTTC+TGG - chr4.3:41397546-41397565 None:intergenic 35.0%
AAGATTCTGCACATTGTTCT+GGG - chr4.3:41397545-41397564 None:intergenic 35.0%
AATGCTAATGGCAATGGTAA+TGG + chr4.3:41397592-41397611 MS.gene65274:intron 35.0%
ACTACTTGGAAGAATGAACA+AGG + chr4.3:41396484-41396503 MS.gene65274:intron 35.0%
AGACACAAATATGGCTGAAA+TGG + chr4.3:41397762-41397781 MS.gene65274:CDS 35.0%
ATGGCTGAAATGGATCATTT+AGG + chr4.3:41397772-41397791 MS.gene65274:CDS 35.0%
CAAGACTTGCAGAAATATCT+TGG - chr4.3:41397184-41397203 None:intergenic 35.0%
GAACCATATGTATATAGCTG+GGG + chr4.3:41396634-41396653 MS.gene65274:intron 35.0%
GTGCAGAATCTTTACTACAT+GGG + chr4.3:41397553-41397572 MS.gene65274:intron 35.0%
TAAGTGCTTCAAAGAATCCT+TGG - chr4.3:41397516-41397535 None:intergenic 35.0%
TAATGCTAGTGTGAGATCAT+TGG - chr4.3:41397371-41397390 None:intergenic 35.0%
TGCAGAATCTTTACTACATG+GGG + chr4.3:41397554-41397573 MS.gene65274:intron 35.0%
TGGAATCATAGGACATTGAA+GGG - chr4.3:41397351-41397370 None:intergenic 35.0%
TGTAGGAGATATTGGACTAA+AGG + chr4.3:41397202-41397221 MS.gene65274:CDS 35.0%
TGTGAGATCATTGGAATCAT+AGG - chr4.3:41397362-41397381 None:intergenic 35.0%
TGTGCAGAATCTTTACTACA+TGG + chr4.3:41397552-41397571 MS.gene65274:intron 35.0%
TTAGCATCTTCATCTTTGTG+AGG + chr4.3:41396710-41396729 MS.gene65274:intron 35.0%
TTGGAATCATAGGACATTGA+AGG - chr4.3:41397352-41397371 None:intergenic 35.0%
TTTCCATGGCAGTTCAATAA+TGG + chr4.3:41397739-41397758 MS.gene65274:CDS 35.0%
! GATATTTCTGCAAGTCTTGT+AGG + chr4.3:41397185-41397204 MS.gene65274:CDS 35.0%
!! AGGATTCTTTGAAGCACTTA+TGG + chr4.3:41397516-41397535 MS.gene65274:intron 35.0%
!! GGATTCTTTGAAGCACTTAT+GGG + chr4.3:41397517-41397536 MS.gene65274:intron 35.0%
!! GTTGATAATGGCAATGCTAA+TGG + chr4.3:41397580-41397599 MS.gene65274:intron 35.0%
!! GTTGGTTTGGTTTCCTAAAA+TGG - chr4.3:41397443-41397462 None:intergenic 35.0%
AACCATTCCTCAAACAACCA+AGG + chr4.3:41397496-41397515 MS.gene65274:intron 40.0%
AAGAATACCACATGAGGTGT+TGG - chr4.3:41397461-41397480 None:intergenic 40.0%
AATGGCAATGCTAATGGCAA+TGG + chr4.3:41397586-41397605 MS.gene65274:intron 40.0%
AATGGTCTCACTTCATCTTG+GGG + chr4.3:41397808-41397827 MS.gene65274:CDS 40.0%
ACACTAAGGAATGTTCCTGT+TGG + chr4.3:41397229-41397248 MS.gene65274:CDS 40.0%
AGCATTAGCTAGGCTTCAAA+AGG + chr4.3:41397384-41397403 MS.gene65274:CDS 40.0%
AGGAGATATTGGACTAAAGG+TGG + chr4.3:41397205-41397224 MS.gene65274:CDS 40.0%
ATCATTTAGGAAGAGCTGGT+TGG + chr4.3:41397785-41397804 MS.gene65274:CDS 40.0%
ATCCTTGGTTGTTTGAGGAA+TGG - chr4.3:41397501-41397520 None:intergenic 40.0%
ATCTCACACTAGCATTAGCT+AGG + chr4.3:41397374-41397393 MS.gene65274:CDS 40.0%
ATGGATCATTTAGGAAGAGC+TGG + chr4.3:41397781-41397800 MS.gene65274:CDS 40.0%
ATTGAAGGGAAACAAGTGAG+TGG - chr4.3:41397337-41397356 None:intergenic 40.0%
CATCATCAGCTTCATCAAAG+AGG + chr4.3:41397278-41397297 MS.gene65274:CDS 40.0%
GAATGGTCTCACTTCATCTT+GGG + chr4.3:41397807-41397826 MS.gene65274:CDS 40.0%
GCAAATGTCTAACCAGTCAT+TGG + chr4.3:41397024-41397043 MS.gene65274:intron 40.0%
GCAGAATCTTTACTACATGG+GGG + chr4.3:41397555-41397574 MS.gene65274:intron 40.0%
GCATTAGCTAGGCTTCAAAA+GGG + chr4.3:41397385-41397404 MS.gene65274:CDS 40.0%
TAAGGAATGTTCCTGTTGGT+GGG + chr4.3:41397233-41397252 MS.gene65274:CDS 40.0%
TCATGAAGGACAAAGATGGA+AGG - chr4.3:41396455-41396474 None:intergenic 40.0%
TCTCCATTATTGAACTGCCA+TGG - chr4.3:41397745-41397764 None:intergenic 40.0%
TTAACATGCTCTCCAATGAC+TGG - chr4.3:41397039-41397058 None:intergenic 40.0%
TTGATGGAATAAAGTCAGGG+AGG + chr4.3:41396671-41396690 MS.gene65274:intron 40.0%
! GCAAGTCTTGTAGGAGATAT+TGG + chr4.3:41397194-41397213 MS.gene65274:CDS 40.0%
! GGAAACAAGTGAGTGGATTT+GGG - chr4.3:41397330-41397349 None:intergenic 40.0%
! TCTTCTTTGAAGAACACCCT+TGG + chr4.3:41396534-41396553 MS.gene65274:intron 40.0%
! TTCTTCTAGTGGACAACAAC+AGG + chr4.3:41396348-41396367 MS.gene65274:CDS 40.0%
!! GGATTTGGGTTGGTTTGAAA+TGG - chr4.3:41397316-41397335 None:intergenic 40.0%
!! GTGTTGTTTGAGTCACATCT+TGG - chr4.3:41397124-41397143 None:intergenic 40.0%
!!! CATTTTAGAGCTTCTGGTTG+AGG - chr4.3:41397100-41397119 None:intergenic 40.0%
!!! CTTGGACATTTTAGAGCTTC+TGG - chr4.3:41397106-41397125 None:intergenic 40.0%
AACCAAACCAACACCTCATG+TGG + chr4.3:41397451-41397470 MS.gene65274:intron 45.0%
AAGGAATGTTCCTGTTGGTG+GGG + chr4.3:41397234-41397253 MS.gene65274:CDS 45.0%
ACATTAGGGGGCTATTGAGA+AGG - chr4.3:41397840-41397859 None:intergenic 45.0%
AGGCCCCAGCTATATACATA+TGG - chr4.3:41396640-41396659 None:intergenic 45.0%
AGGCTTCAAAAGGGTCAACT+TGG + chr4.3:41397394-41397413 MS.gene65274:intron 45.0%
ATGGTCTCACTTCATCTTGG+GGG + chr4.3:41397809-41397828 MS.gene65274:CDS 45.0%
CTAAGGAATGTTCCTGTTGG+TGG + chr4.3:41397232-41397251 MS.gene65274:CDS 45.0%
GAATCTTTACTACATGGGGG+AGG + chr4.3:41397558-41397577 MS.gene65274:intron 45.0%
GGAATGGTCTCACTTCATCT+TGG + chr4.3:41397806-41397825 MS.gene65274:CDS 45.0%
GGACTAAAGGTGGTACACTA+AGG + chr4.3:41397215-41397234 MS.gene65274:CDS 45.0%
TTAGGAAGAGCTGGTTGGAA+TGG + chr4.3:41397790-41397809 MS.gene65274:CDS 45.0%
! ACAAGTGAGTGGATTTGGGT+TGG - chr4.3:41397326-41397345 None:intergenic 45.0%
! ATGGGGATGGATTCTTCTAG+TGG + chr4.3:41396337-41396356 MS.gene65274:CDS 45.0%
! GGGAAACAAGTGAGTGGATT+TGG - chr4.3:41397331-41397350 None:intergenic 45.0%
! TTTAGAGCTTCTGGTTGAGG+AGG - chr4.3:41397097-41397116 None:intergenic 45.0%
!! TACATGGGGGAGGTTGATAA+TGG + chr4.3:41397568-41397587 MS.gene65274:intron 45.0%
!! TACCACATGAGGTGTTGGTT+TGG - chr4.3:41397456-41397475 None:intergenic 45.0%
!!! GGGTGTTGTTAGGTTTTCCA+TGG + chr4.3:41397725-41397744 MS.gene65274:intron 45.0%
!! TAAAAAGAAAAATTTATTAA+TGG + chr4.3:41396753-41396772 MS.gene65274:intron 5.0%
! AGGAATGTTCCTGTTGGTGG+GGG + chr4.3:41397235-41397254 MS.gene65274:CDS 50.0%
!! GCTTCTGGTTGAGGAGGTTT+TGG - chr4.3:41397091-41397110 None:intergenic 50.0%
CTCACTTCATCTTGGGGGCA+TGG + chr4.3:41397814-41397833 MS.gene65274:CDS 55.0%
TTTCTACACCCCCCACCAAC+AGG - chr4.3:41397247-41397266 None:intergenic 55.0%
! GGAATGTTCCTGTTGGTGGG+GGG + chr4.3:41397236-41397255 MS.gene65274:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 41396337 41397861 41396337 ID=MS.gene65274
chr4.3 mRNA 41396337 41397861 41396337 ID=MS.gene65274.t1;Parent=MS.gene65274
chr4.3 exon 41396337 41396369 41396337 ID=MS.gene65274.t1.exon1;Parent=MS.gene65274.t1
chr4.3 CDS 41396337 41396369 41396337 ID=cds.MS.gene65274.t1;Parent=MS.gene65274.t1
chr4.3 exon 41397025 41397406 41397025 ID=MS.gene65274.t1.exon2;Parent=MS.gene65274.t1
chr4.3 CDS 41397025 41397406 41397025 ID=cds.MS.gene65274.t1;Parent=MS.gene65274.t1
chr4.3 exon 41397737 41397861 41397737 ID=MS.gene65274.t1.exon3;Parent=MS.gene65274.t1
chr4.3 CDS 41397737 41397861 41397737 ID=cds.MS.gene65274.t1;Parent=MS.gene65274.t1
Gene Sequence

>MS.gene65274

ATGGGGATGGATTCTTCTAGTGGACAACAACAGGTGAAATATTTATTTCATATCATATATAATTATCATGCATGTAGTCATGTAATATTTATTAACGTGGCTTCTTTGATTTTTGCCTTCCATCTTTGTCCTTCATGAATTTTTAGTACTACTTGGAAGAATGAACAAGGAACCTATTATTTTCCTTAATTTTTGTTTCTTCTTTGAAGAACACCCTTGGATGAAATAAAATTAGTAAAGTTATTTCCCTCTACAATTTTATTATGTATATACTATACTACTCATGGTGTTTCCTATGAACCATATGTATATAGCTGGGGCCTAGAATTAATATTTGATGGAATAAAGTCAGGGAGGATAGAAAGAAAAAAAATTAGCATCTTCATCTTTGTGAGGCCCCTCTAGATCAAATAAAGTAAAAAGAAAAATTTATTAATGGATGTACATGTTGGGAGTGAGTAATTTATTCAGTTTCGTTTTAGTTCTTAACATTATGTTTGAATGTGGAGTTTCAATAATACTAAGACGACACACAATTTTGTCAAATTCGCAATGATTTCAAAAATCAATTAACTCTTTGATTTTTTACTTGTGTACACATATGTATGTACTATGTATAGATAGATCGCAAAAATTTGTACTATTTGGTTTTCATGTCCACTAAATTTGTTGTGTTGTGTTGTTGCAGCAAATGTCTAACCAGTCATTGGAGAGCATGTTAACATGTTCAAAAGCAGAACAAGACAAAAAGCCAAAACCTCCTCAACCAGAAGCTCTAAAATGTCCAAGATGTGACTCAAACAACACCAAATTTTGTTACTACAACAATTACAGTCTTTCTCAACCAAGATATTTCTGCAAGTCTTGTAGGAGATATTGGACTAAAGGTGGTACACTAAGGAATGTTCCTGTTGGTGGGGGGTGTAGAAAGAACAAAAGATCATCATCAGCTTCATCAAAGAGGTCTCAAGATCAACCATTTCAAACCAACCCAAATCCACTCACTTGTTTCCCTTCAATGTCCTATGATTCCAATGATCTCACACTAGCATTAGCTAGGCTTCAAAAGGGTCAACTTGGATTTGATCATGAACATGATTTTTCCATTTTAGGAAACCAAACCAACACCTCATGTGGTATTCTTAACAATCATGGCATGAACCATTCCTCAAACAACCAAGGATTCTTTGAAGCACTTATGGGATCCCAGAACAATGTGCAGAATCTTTACTACATGGGGGAGGTTGATAATGGCAATGCTAATGGCAATGGTAATGGTGAGATGATGTTACCTTATGATCATGAAATGAGCATTGCAACTACTCAAGCAGTTACAGTTACTACTATGAAACAAGAAATGTGTAATGTTAGGGAACAAAATGAAAATAGGGTGTTGTTAGGTTTTCCATGGCAGTTCAATAATGGAGACACAAATATGGCTGAAATGGATCATTTAGGAAGAGCTGGTTGGAATGGTCTCACTTCATCTTGGGGGCATGGCCTTCTCAATAGCCCCCTAATGTAG
Protein sequence

>MS.gene65274.t1

MGMDSSSGQQQQMSNQSLESMLTCSKAEQDKKPKPPQPEALKCPRCDSNNTKFCYYNNYSLSQPRYFCKSCRRYWTKGGTLRNVPVGGGCRKNKRSSSASSKRSQDQPFQTNPNPLTCFPSMSYDSNDLTLALARLQKGFPWQFNNGDTNMAEMDHLGRAGWNGLTSSWGHGLLNSPLM