Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65557.t1 | XP_013451665.1 | 98.7 | 385 | 4 | 1 | 1 | 385 | 31 | 414 | 6.60E-217 | 763.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65557.t1 | O82030 | 84.6 | 358 | 55 | 0 | 24 | 381 | 52 | 409 | 2.5e-180 | 632.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65557.t1 | A0A072U7F9 | 98.7 | 385 | 4 | 1 | 1 | 385 | 1 | 384 | 4.8e-217 | 763.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene015841 | MS.gene65557 | PPI |
| MS.gene049051 | MS.gene65557 | PPI |
| MS.gene05967 | MS.gene65557 | PPI |
| MS.gene01884 | MS.gene65557 | PPI |
| MS.gene20137 | MS.gene65557 | PPI |
| MS.gene00843 | MS.gene65557 | PPI |
| MS.gene047966 | MS.gene65557 | PPI |
| MS.gene063343 | MS.gene65557 | PPI |
| MS.gene049052 | MS.gene65557 | PPI |
| MS.gene66388 | MS.gene65557 | PPI |
| MS.gene002926 | MS.gene65557 | PPI |
| MS.gene030337 | MS.gene65557 | PPI |
| MS.gene65557 | MS.gene75024 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65557.t1 | MTR_6g033540 | 98.701 | 385 | 4 | 1 | 1 | 385 | 31 | 414 | 0.0 | 776 |
| MS.gene65557.t1 | MTR_6g033540 | 98.701 | 385 | 4 | 1 | 1 | 385 | 1 | 384 | 0.0 | 775 |
| MS.gene65557.t1 | MTR_6g033600 | 94.656 | 262 | 13 | 1 | 118 | 379 | 53 | 313 | 2.46e-180 | 504 |
| MS.gene65557.t1 | MTR_3g072670 | 26.073 | 303 | 185 | 13 | 102 | 374 | 138 | 431 | 3.00e-14 | 74.3 |
| MS.gene65557.t1 | MTR_3g072680 | 24.138 | 290 | 186 | 11 | 111 | 374 | 107 | 388 | 8.61e-13 | 69.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65557.t1 | AT5G10330 | 82.873 | 362 | 62 | 0 | 23 | 384 | 55 | 416 | 0.0 | 631 |
| MS.gene65557.t1 | AT5G10330 | 82.873 | 362 | 62 | 0 | 23 | 384 | 55 | 416 | 0.0 | 631 |
| MS.gene65557.t1 | AT1G71920 | 82.873 | 362 | 62 | 0 | 23 | 384 | 55 | 416 | 0.0 | 631 |
| MS.gene65557.t1 | AT1G71920 | 82.873 | 362 | 62 | 0 | 23 | 384 | 55 | 416 | 0.0 | 631 |
| MS.gene65557.t1 | AT5G10330 | 83.056 | 360 | 61 | 0 | 25 | 384 | 2 | 361 | 0.0 | 627 |
| MS.gene65557.t1 | AT5G10330 | 83.056 | 360 | 61 | 0 | 25 | 384 | 2 | 361 | 0.0 | 627 |
| MS.gene65557.t1 | AT1G71920 | 83.056 | 360 | 61 | 0 | 25 | 384 | 2 | 361 | 0.0 | 627 |
| MS.gene65557.t1 | AT1G71920 | 82.044 | 362 | 63 | 1 | 23 | 384 | 55 | 414 | 0.0 | 621 |
| MS.gene65557.t1 | AT5G10330 | 82.178 | 303 | 54 | 0 | 82 | 384 | 1 | 303 | 0.0 | 525 |
| MS.gene65557.t1 | AT5G10330 | 82.178 | 303 | 54 | 0 | 82 | 384 | 1 | 303 | 0.0 | 525 |
| MS.gene65557.t1 | AT5G10330 | 82.178 | 303 | 54 | 0 | 82 | 384 | 1 | 303 | 0.0 | 525 |
| MS.gene65557.t1 | AT5G10330 | 82.178 | 303 | 54 | 0 | 82 | 384 | 1 | 303 | 0.0 | 525 |
| MS.gene65557.t1 | AT1G71920 | 82.178 | 303 | 54 | 0 | 82 | 384 | 1 | 303 | 0.0 | 525 |
| MS.gene65557.t1 | AT1G71920 | 82.178 | 303 | 54 | 0 | 82 | 384 | 1 | 303 | 0.0 | 525 |
Find 101 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGACAACATCTTCGGAAAT+TGG | 0.204477 | 6.4:-5555885 | MS.gene65557:CDS |
| ACAAACCTCAAAAGGCAAAA+TGG | 0.253126 | 6.4:+5555850 | None:intergenic |
| GAATTTGATGCTGCAGTTAA+TGG | 0.283406 | 6.4:-5554082 | MS.gene65557:CDS |
| CCGAAGATGTTGTCTGATAA+AGG | 0.285807 | 6.4:+5555892 | None:intergenic |
| TGAGCATAATCAAATATCTT+TGG | 0.293288 | 6.4:-5549448 | MS.gene65557:CDS |
| GCAGCTCTTGCCCAAGATTC+AGG | 0.296333 | 6.4:-5554346 | MS.gene65557:CDS |
| GCCGGACTTCGTGTGGGATA+TGG | 0.297605 | 6.4:-5549482 | MS.gene65557:CDS |
| GCAGCAACAGATACATTATA+TGG | 0.302128 | 6.4:+5549413 | None:intergenic |
| TTCATCCAGCACCACCAGTA+TGG | 0.313584 | 6.4:+5550326 | None:intergenic |
| GAATCTGAATATATTCTTGT+AGG | 0.316423 | 6.4:-5554319 | MS.gene65557:CDS |
| TCCACGTTCAAGCTGAAATC+TGG | 0.326703 | 6.4:+5553901 | None:intergenic |
| CAGATTCAAGGCCTGAATCT+TGG | 0.326756 | 6.4:+5554335 | None:intergenic |
| GCACGCAATCGGCGGCTCTC+TGG | 0.338634 | 6.4:+5554367 | None:intergenic |
| GATGCTAATGAAAATCCTTA+TGG | 0.339478 | 6.4:-5555085 | MS.gene65557:CDS |
| ATTCTTTGTGAGGTTACATC+TGG | 0.352253 | 6.4:-5548742 | MS.gene65557:CDS |
| ACAGGAAGACCTAGCAGAAA+TGG | 0.371399 | 6.4:-5547951 | MS.gene65557:intron |
| ATTGCAAAATCCGACATATC+TGG | 0.384965 | 6.4:-5549369 | MS.gene65557:CDS |
| CCTCTCCATGTTTCTACTAC+TGG | 0.391529 | 6.4:-5555920 | MS.gene65557:CDS |
| GATACTTGAGCTTCCCATAC+TGG | 0.393982 | 6.4:-5550340 | MS.gene65557:CDS |
| CAAGAATATATTCAGATTCA+AGG | 0.402850 | 6.4:+5554323 | None:intergenic |
| TGTGACAATGGCTTCTTCAA+TGG | 0.403480 | 6.4:-5555978 | None:intergenic |
| AATTCATACATTGTGAAAGT+TGG | 0.413250 | 6.4:+5554100 | None:intergenic |
| TCTGGATCCGGATAGACATA+TGG | 0.414258 | 6.4:+5554385 | None:intergenic |
| TACCTCCCATCTGGATTGTT+CGG | 0.415385 | 6.4:+5553823 | None:intergenic |
| TCCATATCCCACACGAAGTC+CGG | 0.417449 | 6.4:+5549481 | None:intergenic |
| AATGGCTTGGAAACGGCTTA+AGG | 0.418932 | 6.4:+5548773 | None:intergenic |
| AAGCTGAAATCTGGCCTCCT+TGG | 0.422105 | 6.4:+5553910 | None:intergenic |
| AGATTCAAGGCCTGAATCTT+GGG | 0.422267 | 6.4:+5554336 | None:intergenic |
| CAAACCTCAAAAGGCAAAAT+GGG | 0.426236 | 6.4:+5555851 | None:intergenic |
| CCTTTATCAGACAACATCTT+CGG | 0.430296 | 6.4:-5555892 | MS.gene65557:CDS |
| TACAGTTGTGTTCTTGATCC+CGG | 0.436182 | 6.4:-5554145 | MS.gene65557:intron |
| AATCGGCGGCTCTCTGGATC+CGG | 0.439573 | 6.4:+5554373 | None:intergenic |
| GGGCAAGAGCTGCACGCAAT+CGG | 0.442236 | 6.4:+5554356 | None:intergenic |
| TTGACTTGTTCAACAGAAGA+AGG | 0.450183 | 6.4:+5555950 | None:intergenic |
| ATAAGCACATCTGTGTGTTC+AGG | 0.460091 | 6.4:+5547863 | None:intergenic |
| ATGACAATCTGATTGTTCTT+CGG | 0.466193 | 6.4:-5550251 | MS.gene65557:CDS |
| AAAGGCAAAATGGGTTGATA+AGG | 0.472832 | 6.4:+5555860 | None:intergenic |
| AGGAAACAACAAACCTCAAA+AGG | 0.483346 | 6.4:+5555842 | None:intergenic |
| GCTTCCCATACTGGTGGTGC+TGG | 0.484303 | 6.4:-5550331 | MS.gene65557:CDS |
| CTAGACAGTTACCTCCCATC+TGG | 0.485426 | 6.4:+5553814 | None:intergenic |
| ACGATTCCCATATGTCTATC+CGG | 0.490034 | 6.4:-5554392 | MS.gene65557:CDS |
| ATTTAATTGTCAGGTTTAGC+CGG | 0.491059 | 6.4:-5549500 | MS.gene65557:intron |
| AAATTAGAATGGCTTGGAAA+CGG | 0.493347 | 6.4:+5548766 | None:intergenic |
| CAATTGAATCAAAGATGAGT+TGG | 0.500291 | 6.4:-5550284 | MS.gene65557:CDS |
| CAGGAAGACCTAGCAGAAAT+GGG | 0.503599 | 6.4:-5547950 | MS.gene65557:CDS |
| TCATACATTGTGAAAGTTGG+AGG | 0.505853 | 6.4:+5554103 | None:intergenic |
| CACAAAGAATGAAATTAGAA+TGG | 0.506760 | 6.4:+5548755 | None:intergenic |
| TCAGTTCTTTATTGCTGTAG+TGG | 0.507524 | 6.4:+5547915 | None:intergenic |
| AGGTTTAGCCGGACTTCGTG+TGG | 0.508164 | 6.4:-5549489 | MS.gene65557:CDS |
| CAGGCTTCCCAACGGATACT+CGG | 0.518648 | 6.4:+5547882 | None:intergenic |
| TTCTAATTTCATTCTTTGTG+AGG | 0.519797 | 6.4:-5548752 | MS.gene65557:CDS |
| AATTGAATCAAAGATGAGTT+GGG | 0.520370 | 6.4:-5550283 | MS.gene65557:CDS |
| CTTGGAAACGGCTTAAGGAA+TGG | 0.522995 | 6.4:+5548778 | None:intergenic |
| GGAATGGAACTGCCTTCAAA+AGG | 0.523912 | 6.4:+5548794 | None:intergenic |
| CTGGATCCGGATAGACATAT+GGG | 0.524963 | 6.4:+5554386 | None:intergenic |
| ATGGGAATCGTATTGACCCT+AGG | 0.533354 | 6.4:+5554404 | None:intergenic |
| CAAGAGCTGCACGCAATCGG+CGG | 0.534042 | 6.4:+5554359 | None:intergenic |
| TGATAGGTCATGGAAGCCCT+AGG | 0.540736 | 6.4:-5554421 | MS.gene65557:intron |
| ATTAAATGGCTCACCTCTGG+AGG | 0.543452 | 6.4:+5555058 | None:intergenic |
| GGCTACGTCCGAGTATCCGT+TGG | 0.543628 | 6.4:-5547890 | MS.gene65557:CDS |
| TTGCATAAGCAAACTATCAC+AGG | 0.547992 | 6.4:-5547837 | MS.gene65557:CDS |
| AGAATGAAATTAGAATGGCT+TGG | 0.553255 | 6.4:+5548760 | None:intergenic |
| GCAGTCGACAATCTTGTCAC+CGG | 0.554232 | 6.4:+5554126 | None:intergenic |
| TCATCCAGCACCACCAGTAT+GGG | 0.555180 | 6.4:+5550327 | None:intergenic |
| GACGCGTTGGTGAAAGAAAG+AGG | 0.558383 | 6.4:-5548829 | MS.gene65557:CDS |
| TTATTTAAACTGTAGTTCCA+AGG | 0.559966 | 6.4:-5553927 | MS.gene65557:intron |
| AAATGGCTCACCTCTGGAGG+GGG | 0.562961 | 6.4:+5555061 | None:intergenic |
| CAGTCGACAATCTTGTCACC+GGG | 0.564938 | 6.4:+5554127 | None:intergenic |
| GCTACGTCCGAGTATCCGTT+GGG | 0.565723 | 6.4:-5547889 | MS.gene65557:CDS |
| GAACGTGGAACAAATAATAG+AGG | 0.570575 | 6.4:-5553887 | MS.gene65557:CDS |
| TTAAATGGCTCACCTCTGGA+GGG | 0.574585 | 6.4:+5555059 | None:intergenic |
| AATGGAGCACTTGTCATCAA+AGG | 0.576124 | 6.4:-5554064 | MS.gene65557:intron |
| ATAGAATGTCAAAGACGCGT+TGG | 0.579919 | 6.4:-5548842 | MS.gene65557:intron |
| TGGGAATCGTATTGACCCTA+GGG | 0.582341 | 6.4:+5554405 | None:intergenic |
| CATACATTGTGAAAGTTGGA+GGG | 0.583038 | 6.4:+5554104 | None:intergenic |
| GCCAGATTTCAGCTTGAACG+TGG | 0.583579 | 6.4:-5553902 | MS.gene65557:CDS |
| AGCTTATTGATTTGATCATG+CGG | 0.587401 | 6.4:-5554282 | MS.gene65557:intron |
| GGTTTAGCCGGACTTCGTGT+GGG | 0.589264 | 6.4:-5549488 | MS.gene65557:CDS |
| TCTAACTTGACAATATCCTC+AGG | 0.589857 | 6.4:+5555106 | None:intergenic |
| TCATCACACCCATTTCTGCT+AGG | 0.591741 | 6.4:+5547942 | None:intergenic |
| GATCAAATCAATAAGCTCAT+CGG | 0.594362 | 6.4:+5554288 | None:intergenic |
| AGATATTTGATTATGCTCAA+AGG | 0.594612 | 6.4:+5549452 | None:intergenic |
| ACATCTCCGAACAATCCAGA+TGG | 0.598183 | 6.4:-5553829 | MS.gene65557:CDS |
| CGGACATTCAGCAAAAGAGC+TGG | 0.598755 | 6.4:-5550231 | MS.gene65557:intron |
| TAAATGGCTCACCTCTGGAG+GGG | 0.601963 | 6.4:+5555060 | None:intergenic |
| AATCACCAGTAGTAGAAACA+TGG | 0.617123 | 6.4:+5555915 | None:intergenic |
| GATAGGTCATGGAAGCCCTA+GGG | 0.627430 | 6.4:-5554420 | MS.gene65557:intron |
| GAATATATTCTTGTAGGATG+TGG | 0.628826 | 6.4:-5554313 | MS.gene65557:CDS |
| TCTGTTGAACAAGTCAACAA+TGG | 0.628910 | 6.4:-5555944 | MS.gene65557:CDS |
| TACAGCAATAAAGAACTGAA+AGG | 0.637274 | 6.4:-5547911 | MS.gene65557:CDS |
| TCGTCTTGGACGCAAGCCTG+AGG | 0.638192 | 6.4:-5555122 | MS.gene65557:CDS |
| CATCTCCGAACAATCCAGAT+GGG | 0.651841 | 6.4:-5553828 | MS.gene65557:CDS |
| GCAAAATCCGACATATCTGG+AGG | 0.653033 | 6.4:-5549366 | MS.gene65557:intron |
| ACTTGTTCAACAGAAGAAGG+AGG | 0.656267 | 6.4:+5555953 | None:intergenic |
| ACGCGTTGGTGAAAGAAAGA+GGG | 0.657218 | 6.4:-5548828 | MS.gene65557:CDS |
| CGTTGGTGAAAGAAAGAGGG+AGG | 0.665484 | 6.4:-5548825 | MS.gene65557:CDS |
| CTCCGAACAATCCAGATGGG+AGG | 0.665608 | 6.4:-5553825 | MS.gene65557:intron |
| TTTAAACTGTAGTTCCAAGG+AGG | 0.667891 | 6.4:-5553924 | MS.gene65557:intron |
| ACTTGAGCTTCCCATACTGG+TGG | 0.673821 | 6.4:-5550337 | MS.gene65557:CDS |
| TGTGTGTTCAGGCTTCCCAA+CGG | 0.684077 | 6.4:+5547874 | None:intergenic |
| CCAGTAGTAGAAACATGGAG+AGG | 0.741629 | 6.4:+5555920 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 5547793 | 5555993 | 5547793 | ID=MS.gene65557 |
| chr6.4 | mRNA | 5547793 | 5555993 | 5547793 | ID=MS.gene65557.t1;Parent=MS.gene65557 |
| chr6.4 | exon | 5555856 | 5555993 | 5555856 | ID=MS.gene65557.t1.exon1;Parent=MS.gene65557.t1 |
| chr6.4 | CDS | 5555856 | 5555993 | 5555856 | ID=cds.MS.gene65557.t1;Parent=MS.gene65557.t1 |
| chr6.4 | exon | 5555072 | 5555155 | 5555072 | ID=MS.gene65557.t1.exon2;Parent=MS.gene65557.t1 |
| chr6.4 | CDS | 5555072 | 5555155 | 5555072 | ID=cds.MS.gene65557.t1;Parent=MS.gene65557.t1 |
| chr6.4 | exon | 5554283 | 5554437 | 5554283 | ID=MS.gene65557.t1.exon3;Parent=MS.gene65557.t1 |
| chr6.4 | CDS | 5554283 | 5554437 | 5554283 | ID=cds.MS.gene65557.t1;Parent=MS.gene65557.t1 |
| chr6.4 | exon | 5554065 | 5554162 | 5554065 | ID=MS.gene65557.t1.exon4;Parent=MS.gene65557.t1 |
| chr6.4 | CDS | 5554065 | 5554162 | 5554065 | ID=cds.MS.gene65557.t1;Parent=MS.gene65557.t1 |
| chr6.4 | exon | 5553826 | 5553934 | 5553826 | ID=MS.gene65557.t1.exon5;Parent=MS.gene65557.t1 |
| chr6.4 | CDS | 5553826 | 5553934 | 5553826 | ID=cds.MS.gene65557.t1;Parent=MS.gene65557.t1 |
| chr6.4 | exon | 5550232 | 5550389 | 5550232 | ID=MS.gene65557.t1.exon6;Parent=MS.gene65557.t1 |
| chr6.4 | CDS | 5550232 | 5550389 | 5550232 | ID=cds.MS.gene65557.t1;Parent=MS.gene65557.t1 |
| chr6.4 | exon | 5549367 | 5549509 | 5549367 | ID=MS.gene65557.t1.exon7;Parent=MS.gene65557.t1 |
| chr6.4 | CDS | 5549367 | 5549509 | 5549367 | ID=cds.MS.gene65557.t1;Parent=MS.gene65557.t1 |
| chr6.4 | exon | 5548720 | 5548860 | 5548720 | ID=MS.gene65557.t1.exon8;Parent=MS.gene65557.t1 |
| chr6.4 | CDS | 5548720 | 5548860 | 5548720 | ID=cds.MS.gene65557.t1;Parent=MS.gene65557.t1 |
| chr6.4 | exon | 5547793 | 5547969 | 5547793 | ID=MS.gene65557.t1.exon9;Parent=MS.gene65557.t1 |
| chr6.4 | CDS | 5547793 | 5547969 | 5547793 | ID=cds.MS.gene65557.t1;Parent=MS.gene65557.t1 |
| Gene Sequence |
| Protein sequence |
