Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66291.t1 | XP_024641792.1 | 94.1 | 762 | 20 | 2 | 1 | 737 | 1 | 762 | 0.00E+00 | 1527.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66291.t1 | Q764T8 | 83.4 | 758 | 95 | 4 | 1 | 730 | 1 | 755 | 0.0e+00 | 1343.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66291.t1 | A0A072UN62 | 94.1 | 762 | 20 | 2 | 1 | 737 | 1 | 762 | 0.0e+00 | 1527.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054713 | MS.gene66291 | 0.808686 | 2.74E-50 | -1.69E-46 |
| MS.gene058042 | MS.gene66291 | 0.818984 | 1.47E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66291.t1 | MTR_6g093180 | 94.094 | 762 | 20 | 2 | 1 | 737 | 1 | 762 | 0.0 | 1492 |
| MS.gene66291.t1 | MTR_6g034985 | 78.478 | 762 | 138 | 3 | 1 | 736 | 1 | 762 | 0.0 | 1267 |
| MS.gene66291.t1 | MTR_6g035010 | 75.399 | 752 | 160 | 2 | 1 | 727 | 1 | 752 | 0.0 | 1188 |
| MS.gene66291.t1 | MTR_4g005190 | 59.205 | 755 | 280 | 4 | 1 | 727 | 1 | 755 | 0.0 | 955 |
| MS.gene66291.t1 | MTR_4g005270 | 58.278 | 755 | 287 | 4 | 1 | 727 | 1 | 755 | 0.0 | 946 |
| MS.gene66291.t1 | MTR_0013s0230 | 57.672 | 756 | 291 | 5 | 1 | 727 | 1 | 756 | 0.0 | 937 |
| MS.gene66291.t1 | MTR_8g016975 | 56.053 | 760 | 306 | 4 | 1 | 732 | 1 | 760 | 0.0 | 928 |
| MS.gene66291.t1 | MTR_5g008810 | 58.940 | 755 | 282 | 5 | 1 | 728 | 1 | 754 | 0.0 | 922 |
| MS.gene66291.t1 | MTR_6g088140 | 55.526 | 760 | 286 | 8 | 1 | 728 | 1 | 740 | 0.0 | 882 |
| MS.gene66291.t1 | MTR_6g093100 | 55.097 | 775 | 300 | 10 | 1 | 728 | 1 | 774 | 0.0 | 880 |
| MS.gene66291.t1 | MTR_8g016960 | 53.236 | 757 | 319 | 6 | 1 | 728 | 1 | 751 | 0.0 | 879 |
| MS.gene66291.t1 | MTR_7g062020 | 54.368 | 767 | 310 | 7 | 1 | 728 | 1 | 766 | 0.0 | 877 |
| MS.gene66291.t1 | MTR_8g017020 | 52.500 | 760 | 299 | 6 | 1 | 732 | 1 | 726 | 0.0 | 855 |
| MS.gene66291.t1 | MTR_7g034535 | 57.742 | 691 | 265 | 4 | 64 | 727 | 1 | 691 | 0.0 | 853 |
| MS.gene66291.t1 | MTR_4g005190 | 58.445 | 669 | 250 | 4 | 1 | 641 | 1 | 669 | 0.0 | 830 |
| MS.gene66291.t1 | MTR_3g436160 | 58.471 | 667 | 247 | 4 | 87 | 727 | 32 | 694 | 0.0 | 820 |
| MS.gene66291.t1 | MTR_6g088140 | 54.167 | 672 | 257 | 7 | 1 | 641 | 1 | 652 | 0.0 | 757 |
| MS.gene66291.t1 | MTR_8g016940 | 55.556 | 270 | 118 | 1 | 463 | 732 | 2 | 269 | 2.68e-102 | 315 |
| MS.gene66291.t1 | MTR_8g016570 | 61.290 | 62 | 24 | 0 | 667 | 728 | 28 | 89 | 7.64e-20 | 86.7 |
| MS.gene66291.t1 | MTR_8g017040 | 57.143 | 70 | 28 | 1 | 1 | 68 | 1 | 70 | 5.28e-19 | 84.0 |
| MS.gene66291.t1 | MTR_3g436170 | 45.679 | 81 | 42 | 1 | 1 | 79 | 1 | 81 | 2.92e-17 | 77.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66291.t1 | AT2G07050 | 59.367 | 758 | 280 | 5 | 1 | 731 | 1 | 757 | 0.0 | 964 |
| MS.gene66291.t1 | AT1G78960 | 56.316 | 760 | 303 | 5 | 1 | 731 | 1 | 760 | 0.0 | 910 |
| MS.gene66291.t1 | AT1G78960 | 56.316 | 760 | 303 | 5 | 1 | 731 | 1 | 760 | 0.0 | 910 |
| MS.gene66291.t1 | AT1G78960 | 56.316 | 760 | 303 | 5 | 1 | 731 | 1 | 760 | 0.0 | 910 |
| MS.gene66291.t1 | AT1G78955 | 57.407 | 756 | 293 | 4 | 1 | 727 | 1 | 756 | 0.0 | 909 |
| MS.gene66291.t1 | AT1G78950 | 56.217 | 756 | 302 | 4 | 1 | 727 | 10 | 765 | 0.0 | 897 |
| MS.gene66291.t1 | AT1G78950 | 56.217 | 756 | 302 | 4 | 1 | 727 | 1 | 756 | 0.0 | 896 |
| MS.gene66291.t1 | AT2G07050 | 60.241 | 664 | 237 | 4 | 94 | 731 | 4 | 666 | 0.0 | 863 |
| MS.gene66291.t1 | AT1G66960 | 54.183 | 753 | 316 | 4 | 1 | 724 | 1 | 753 | 0.0 | 863 |
| MS.gene66291.t1 | AT1G66960 | 54.183 | 753 | 316 | 4 | 1 | 724 | 1 | 753 | 0.0 | 863 |
| MS.gene66291.t1 | AT1G66960 | 54.183 | 753 | 316 | 4 | 1 | 724 | 10 | 762 | 0.0 | 863 |
| MS.gene66291.t1 | AT1G66960 | 54.183 | 753 | 316 | 4 | 1 | 724 | 1 | 753 | 0.0 | 863 |
| MS.gene66291.t1 | AT1G78960 | 56.293 | 723 | 287 | 5 | 1 | 694 | 1 | 723 | 0.0 | 862 |
| MS.gene66291.t1 | AT1G78970 | 54.954 | 757 | 309 | 6 | 1 | 728 | 1 | 754 | 0.0 | 859 |
| MS.gene66291.t1 | AT1G78970 | 54.954 | 757 | 309 | 6 | 1 | 728 | 1 | 754 | 0.0 | 859 |
| MS.gene66291.t1 | AT1G78970 | 54.954 | 757 | 309 | 6 | 1 | 728 | 1 | 754 | 0.0 | 859 |
| MS.gene66291.t1 | AT1G78955 | 57.391 | 690 | 268 | 3 | 64 | 727 | 2 | 691 | 0.0 | 835 |
| MS.gene66291.t1 | AT3G45130 | 56.596 | 758 | 297 | 7 | 1 | 729 | 1 | 755 | 0.0 | 822 |
| MS.gene66291.t1 | AT3G45130 | 56.596 | 758 | 297 | 7 | 1 | 729 | 1 | 755 | 0.0 | 822 |
| MS.gene66291.t1 | AT3G45130 | 56.596 | 758 | 297 | 7 | 1 | 729 | 1 | 755 | 0.0 | 822 |
| MS.gene66291.t1 | AT3G45130 | 56.596 | 758 | 297 | 7 | 1 | 729 | 1 | 755 | 0.0 | 822 |
| MS.gene66291.t1 | AT3G45130 | 56.596 | 758 | 297 | 7 | 1 | 729 | 1 | 755 | 0.0 | 822 |
| MS.gene66291.t1 | AT1G78955 | 56.621 | 657 | 259 | 3 | 64 | 694 | 2 | 658 | 0.0 | 779 |
| MS.gene66291.t1 | AT1G78500 | 49.736 | 758 | 346 | 7 | 1 | 723 | 1 | 758 | 0.0 | 764 |
| MS.gene66291.t1 | AT1G78500 | 49.736 | 758 | 346 | 7 | 1 | 723 | 1 | 758 | 0.0 | 764 |
| MS.gene66291.t1 | AT1G78960 | 57.635 | 609 | 232 | 3 | 149 | 731 | 1 | 609 | 0.0 | 762 |
| MS.gene66291.t1 | AT5G36150 | 47.884 | 756 | 365 | 5 | 1 | 727 | 1 | 756 | 0.0 | 761 |
| MS.gene66291.t1 | AT5G36150 | 47.884 | 756 | 365 | 5 | 1 | 727 | 1 | 756 | 0.0 | 761 |
| MS.gene66291.t1 | AT5G48010 | 47.375 | 762 | 361 | 9 | 1 | 725 | 1 | 759 | 0.0 | 738 |
| MS.gene66291.t1 | AT4G15370 | 47.354 | 756 | 358 | 7 | 1 | 723 | 1 | 749 | 0.0 | 737 |
| MS.gene66291.t1 | AT4G15370 | 47.167 | 759 | 358 | 7 | 1 | 723 | 1 | 752 | 0.0 | 735 |
| MS.gene66291.t1 | AT4G15340 | 47.320 | 765 | 369 | 7 | 1 | 731 | 1 | 765 | 0.0 | 734 |
| MS.gene66291.t1 | AT5G42600 | 47.012 | 753 | 368 | 7 | 1 | 723 | 1 | 752 | 0.0 | 724 |
| MS.gene66291.t1 | AT5G48010 | 46.588 | 762 | 359 | 10 | 1 | 725 | 1 | 751 | 0.0 | 716 |
| MS.gene66291.t1 | AT1G78955 | 58.520 | 581 | 215 | 3 | 173 | 727 | 1 | 581 | 0.0 | 711 |
| MS.gene66291.t1 | AT1G78955 | 56.219 | 603 | 238 | 3 | 64 | 640 | 2 | 604 | 0.0 | 709 |
| MS.gene66291.t1 | AT3G29255 | 43.617 | 752 | 335 | 9 | 2 | 723 | 6 | 698 | 0.0 | 636 |
| MS.gene66291.t1 | AT4G15340 | 46.127 | 581 | 279 | 7 | 1 | 547 | 1 | 581 | 0.0 | 533 |
| MS.gene66291.t1 | AT1G78480 | 34.615 | 156 | 87 | 5 | 49 | 197 | 29 | 176 | 1.82e-18 | 84.7 |
Find 0 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 41772 | gene | 11544 | 24480 | 11544 | ID=MS.gene66291 |
| 41772 | mRNA | 11544 | 24480 | 11544 | ID=MS.gene66291.t1;Parent=MS.gene66291 |
| 41772 | exon | 24286 | 24480 | 24286 | ID=MS.gene66291.t1.exon1;Parent=MS.gene66291.t1 |
| 41772 | CDS | 24286 | 24480 | 24286 | ID=cds.MS.gene66291.t1;Parent=MS.gene66291.t1 |
| 41772 | exon | 16398 | 16583 | 16398 | ID=MS.gene66291.t1.exon2;Parent=MS.gene66291.t1 |
| 41772 | CDS | 16398 | 16583 | 16398 | ID=cds.MS.gene66291.t1;Parent=MS.gene66291.t1 |
| 41772 | exon | 16118 | 16207 | 16118 | ID=MS.gene66291.t1.exon3;Parent=MS.gene66291.t1 |
| 41772 | CDS | 16118 | 16207 | 16118 | ID=cds.MS.gene66291.t1;Parent=MS.gene66291.t1 |
| 41772 | exon | 14564 | 14761 | 14564 | ID=MS.gene66291.t1.exon4;Parent=MS.gene66291.t1 |
| 41772 | CDS | 14564 | 14761 | 14564 | ID=cds.MS.gene66291.t1;Parent=MS.gene66291.t1 |
| 41772 | exon | 14386 | 14470 | 14386 | ID=MS.gene66291.t1.exon5;Parent=MS.gene66291.t1 |
| 41772 | CDS | 14386 | 14470 | 14386 | ID=cds.MS.gene66291.t1;Parent=MS.gene66291.t1 |
| 41772 | exon | 14122 | 14288 | 14122 | ID=MS.gene66291.t1.exon6;Parent=MS.gene66291.t1 |
| 41772 | CDS | 14122 | 14288 | 14122 | ID=cds.MS.gene66291.t1;Parent=MS.gene66291.t1 |
| 41772 | exon | 13819 | 13996 | 13819 | ID=MS.gene66291.t1.exon7;Parent=MS.gene66291.t1 |
| 41772 | CDS | 13819 | 13996 | 13819 | ID=cds.MS.gene66291.t1;Parent=MS.gene66291.t1 |
| 41772 | exon | 13694 | 13776 | 13694 | ID=MS.gene66291.t1.exon8;Parent=MS.gene66291.t1 |
| 41772 | CDS | 13694 | 13776 | 13694 | ID=cds.MS.gene66291.t1;Parent=MS.gene66291.t1 |
| 41772 | exon | 13232 | 13342 | 13232 | ID=MS.gene66291.t1.exon9;Parent=MS.gene66291.t1 |
| 41772 | CDS | 13232 | 13342 | 13232 | ID=cds.MS.gene66291.t1;Parent=MS.gene66291.t1 |
| 41772 | exon | 13100 | 13198 | 13100 | ID=MS.gene66291.t1.exon10;Parent=MS.gene66291.t1 |
| 41772 | CDS | 13100 | 13198 | 13100 | ID=cds.MS.gene66291.t1;Parent=MS.gene66291.t1 |
| 41772 | exon | 12842 | 12997 | 12842 | ID=MS.gene66291.t1.exon11;Parent=MS.gene66291.t1 |
| 41772 | CDS | 12842 | 12997 | 12842 | ID=cds.MS.gene66291.t1;Parent=MS.gene66291.t1 |
| 41772 | exon | 12692 | 12738 | 12692 | ID=MS.gene66291.t1.exon12;Parent=MS.gene66291.t1 |
| 41772 | CDS | 12692 | 12738 | 12692 | ID=cds.MS.gene66291.t1;Parent=MS.gene66291.t1 |
| 41772 | exon | 12435 | 12578 | 12435 | ID=MS.gene66291.t1.exon13;Parent=MS.gene66291.t1 |
| 41772 | CDS | 12435 | 12578 | 12435 | ID=cds.MS.gene66291.t1;Parent=MS.gene66291.t1 |
| 41772 | exon | 12119 | 12296 | 12119 | ID=MS.gene66291.t1.exon14;Parent=MS.gene66291.t1 |
| 41772 | CDS | 12119 | 12296 | 12119 | ID=cds.MS.gene66291.t1;Parent=MS.gene66291.t1 |
| 41772 | exon | 11966 | 12046 | 11966 | ID=MS.gene66291.t1.exon15;Parent=MS.gene66291.t1 |
| 41772 | CDS | 11966 | 12046 | 11966 | ID=cds.MS.gene66291.t1;Parent=MS.gene66291.t1 |
| 41772 | exon | 11776 | 11859 | 11776 | ID=MS.gene66291.t1.exon16;Parent=MS.gene66291.t1 |
| 41772 | CDS | 11776 | 11859 | 11776 | ID=cds.MS.gene66291.t1;Parent=MS.gene66291.t1 |
| 41772 | exon | 11544 | 11675 | 11544 | ID=MS.gene66291.t1.exon17;Parent=MS.gene66291.t1 |
| 41772 | CDS | 11544 | 11675 | 11544 | ID=cds.MS.gene66291.t1;Parent=MS.gene66291.t1 |
| Gene Sequence |
| Protein sequence |
