Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66619.t1 | XP_013455692.1 | 96 | 50 | 2 | 0 | 101 | 150 | 103 | 152 | 5.30E-18 | 100.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66619.t1 | A0A072UKU9 | 96.0 | 50 | 2 | 0 | 101 | 150 | 103 | 152 | 3.8e-18 | 100.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052358 | MS.gene66619 | -0.814902 | 1.22E-51 | -1.69E-46 |
| MS.gene05548 | MS.gene66619 | 0.824785 | 6.69E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66619.t1 | MTR_4g050130 | 87.162 | 148 | 18 | 1 | 3 | 150 | 6 | 152 | 2.02e-57 | 176 |
| MS.gene66619.t1 | MTR_2g087830 | 38.971 | 136 | 65 | 5 | 15 | 149 | 8 | 126 | 6.98e-26 | 95.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66619.t1 | AT3G11690 | 38.356 | 146 | 71 | 5 | 23 | 149 | 56 | 201 | 5.85e-16 | 72.8 |
| MS.gene66619.t1 | AT5G06380 | 34.211 | 152 | 83 | 4 | 1 | 149 | 1 | 138 | 6.48e-16 | 70.5 |
| MS.gene66619.t1 | AT2G27180 | 40.559 | 143 | 73 | 2 | 10 | 149 | 26 | 159 | 8.80e-14 | 65.1 |
Find 34 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTTTGCGGGAACCGGGTTT+TGG | 0.261947 | 4.3:-49531144 | MS.gene66619:CDS |
| TGGGCACGGTTTGCGGGAAC+CGG | 0.294255 | 4.3:-49531151 | MS.gene66619:CDS |
| GAGATGTGGGCACGGTTTGC+GGG | 0.342993 | 4.3:-49531157 | MS.gene66619:CDS |
| CAGAGATCAAGCAGTGTTGC+TGG | 0.393742 | 4.3:-49531268 | MS.gene66619:CDS |
| GCATTCTGCTGCTGCGCTGC+CGG | 0.408371 | 4.3:+49531474 | None:intergenic |
| TCGTGCGGCGGAGGAGAAAT+CGG | 0.423161 | 4.3:-49531200 | MS.gene66619:CDS |
| GGAGATGTGGGCACGGTTTG+CGG | 0.441228 | 4.3:-49531158 | MS.gene66619:CDS |
| GGGCACGGTTTGCGGGAACC+GGG | 0.458010 | 4.3:-49531150 | MS.gene66619:CDS |
| TGATATTAAGATTGATTCGT+TGG | 0.462200 | 4.3:-49531242 | MS.gene66619:CDS |
| CGTGAAGGATCGTGCGGCGG+AGG | 0.484879 | 4.3:-49531209 | MS.gene66619:CDS |
| CGTGGTAAAAGACGCGGCGT+TGG | 0.530731 | 4.3:-49531505 | MS.gene66619:CDS |
| GAAATCGGAGGAGGTTGCGT+TGG | 0.533846 | 4.3:-49531185 | MS.gene66619:CDS |
| TGAATGAGTGAGAGCGTGGC+TGG | 0.535132 | 4.3:+49531540 | None:intergenic |
| TTTGAAAAGTGCGGTCGGGA+CGG | 0.539525 | 4.3:+49531390 | None:intergenic |
| GGAGGAGGTTGCGTTGGAGA+AGG | 0.558865 | 4.3:-49531179 | MS.gene66619:CDS |
| CCTACGTCGTCTTCGATGAA+CGG | 0.563027 | 4.3:+49531363 | None:intergenic |
| AGAGATCAAGCAGTGTTGCT+GGG | 0.568754 | 4.3:-49531267 | MS.gene66619:CDS |
| TGCGTTGGAGAAGGAGATGT+GGG | 0.586252 | 4.3:-49531170 | MS.gene66619:CDS |
| ATGCTGTTGCTGTCCATGCA+CGG | 0.590289 | 4.3:-49531443 | MS.gene66619:CDS |
| CGCGGCGTTGGAGAAACAGC+CGG | 0.590433 | 4.3:-49531493 | MS.gene66619:CDS |
| ACGACGTTGAGAACCGTGCA+TGG | 0.594359 | 4.3:+49531430 | None:intergenic |
| GGCGGAGGAGAAATCGGAGG+AGG | 0.610740 | 4.3:-49531194 | MS.gene66619:CDS |
| AGAATCACTTCTCCAAAACC+CGG | 0.610774 | 4.3:+49531132 | None:intergenic |
| GTTGGAAGAGCATTTCGTGA+AGG | 0.624024 | 4.3:-49531224 | MS.gene66619:CDS |
| TTGCGTTGGAGAAGGAGATG+TGG | 0.625295 | 4.3:-49531171 | MS.gene66619:CDS |
| TGGAGAAGGAGATGTGGGCA+CGG | 0.638141 | 4.3:-49531165 | MS.gene66619:CDS |
| TTTCGTGAAGGATCGTGCGG+CGG | 0.669525 | 4.3:-49531212 | MS.gene66619:CDS |
| TGCGTGAATGAGTGAGAGCG+TGG | 0.677938 | 4.3:+49531536 | None:intergenic |
| CCGTTCATCGAAGACGACGT+AGG | 0.689307 | 4.3:-49531363 | MS.gene66619:CDS |
| TGCGGCGGAGGAGAAATCGG+AGG | 0.691203 | 4.3:-49531197 | MS.gene66619:CDS |
| GCATTTCGTGAAGGATCGTG+CGG | 0.703359 | 4.3:-49531215 | MS.gene66619:CDS |
| CATTCACGCACAACGCCGCG+TGG | 0.704431 | 4.3:-49531523 | MS.gene66619:CDS |
| ACGCCGCGTGGTAAAAGACG+CGG | 0.716783 | 4.3:-49531511 | MS.gene66619:CDS |
| TCGGGACGGAGTAGACTGCG+AGG | 0.785457 | 4.3:+49531404 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGATATTAAGATTGATTCGT+TGG | - | chr4.3:49531422-49531441 | MS.gene66619:CDS | 25.0% |
| GAAAAACAACGTTAACGATG+TGG | - | chr4.3:49531359-49531378 | MS.gene66619:CDS | 35.0% | |
| !!! | AACGGCTTTTTTGAAAAGTG+CGG | + | chr4.3:49531286-49531305 | None:intergenic | 35.0% |
| AGAATCACTTCTCCAAAACC+CGG | + | chr4.3:49531535-49531554 | None:intergenic | 40.0% | |
| !!! | CTTTTTTGAAAAGTGCGGTC+GGG | + | chr4.3:49531281-49531300 | None:intergenic | 40.0% |
| !!! | GCTTTTTTGAAAAGTGCGGT+CGG | + | chr4.3:49531282-49531301 | None:intergenic | 40.0% |
| ! | AGAGATCAAGCAGTGTTGCT+GGG | - | chr4.3:49531397-49531416 | MS.gene66619:CDS | 45.0% |
| !! | GTTGGAAGAGCATTTCGTGA+AGG | - | chr4.3:49531440-49531459 | MS.gene66619:CDS | 45.0% |
| ATGCTGTTGCTGTCCATGCA+CGG | - | chr4.3:49531221-49531240 | MS.gene66619:CDS | 50.0% | |
| CAGAGATCAAGCAGTGTTGC+TGG | - | chr4.3:49531396-49531415 | MS.gene66619:CDS | 50.0% | |
| CCTACGTCGTCTTCGATGAA+CGG | + | chr4.3:49531304-49531323 | None:intergenic | 50.0% | |
| GCATTTCGTGAAGGATCGTG+CGG | - | chr4.3:49531449-49531468 | MS.gene66619:CDS | 50.0% | |
| TGCGTTGGAGAAGGAGATGT+GGG | - | chr4.3:49531494-49531513 | MS.gene66619:CDS | 50.0% | |
| TTGCGTTGGAGAAGGAGATG+TGG | - | chr4.3:49531493-49531512 | MS.gene66619:CDS | 50.0% | |
| ! | TTTGAAAAGTGCGGTCGGGA+CGG | + | chr4.3:49531277-49531296 | None:intergenic | 50.0% |
| ACGACGTTGAGAACCGTGCA+TGG | + | chr4.3:49531237-49531256 | None:intergenic | 55.0% | |
| CCGTTCATCGAAGACGACGT+AGG | - | chr4.3:49531301-49531320 | MS.gene66619:CDS | 55.0% | |
| GAAATCGGAGGAGGTTGCGT+TGG | - | chr4.3:49531479-49531498 | MS.gene66619:CDS | 55.0% | |
| TGCGTGAATGAGTGAGAGCG+TGG | + | chr4.3:49531131-49531150 | None:intergenic | 55.0% | |
| TGGAGAAGGAGATGTGGGCA+CGG | - | chr4.3:49531499-49531518 | MS.gene66619:CDS | 55.0% | |
| TTTCGTGAAGGATCGTGCGG+CGG | - | chr4.3:49531452-49531471 | MS.gene66619:CDS | 55.0% | |
| ! | TGAATGAGTGAGAGCGTGGC+TGG | + | chr4.3:49531127-49531146 | None:intergenic | 55.0% |
| ACGCCGCGTGGTAAAAGACG+CGG | - | chr4.3:49531153-49531172 | MS.gene66619:CDS | 60.0% | |
| CGTGGTAAAAGACGCGGCGT+TGG | - | chr4.3:49531159-49531178 | MS.gene66619:CDS | 60.0% | |
| GAGATGTGGGCACGGTTTGC+GGG | - | chr4.3:49531507-49531526 | MS.gene66619:CDS | 60.0% | |
| GGAGATGTGGGCACGGTTTG+CGG | - | chr4.3:49531506-49531525 | MS.gene66619:CDS | 60.0% | |
| GGAGGAGGTTGCGTTGGAGA+AGG | - | chr4.3:49531485-49531504 | MS.gene66619:CDS | 60.0% | |
| TCGTGCGGCGGAGGAGAAAT+CGG | - | chr4.3:49531464-49531483 | MS.gene66619:CDS | 60.0% | |
| ! | ACGCCGCGTCTTTTACCACG+CGG | + | chr4.3:49531159-49531178 | None:intergenic | 60.0% |
| ! | GGTTTGCGGGAACCGGGTTT+TGG | - | chr4.3:49531520-49531539 | MS.gene66619:CDS | 60.0% |
| CATTCACGCACAACGCCGCG+TGG | - | chr4.3:49531141-49531160 | MS.gene66619:CDS | 65.0% | |
| GCATTCTGCTGCTGCGCTGC+CGG | + | chr4.3:49531193-49531212 | None:intergenic | 65.0% | |
| GGCGGAGGAGAAATCGGAGG+AGG | - | chr4.3:49531470-49531489 | MS.gene66619:CDS | 65.0% | |
| TCGGGACGGAGTAGACTGCG+AGG | + | chr4.3:49531263-49531282 | None:intergenic | 65.0% | |
| TGCGGCGGAGGAGAAATCGG+AGG | - | chr4.3:49531467-49531486 | MS.gene66619:CDS | 65.0% | |
| TGGGCACGGTTTGCGGGAAC+CGG | - | chr4.3:49531513-49531532 | MS.gene66619:CDS | 65.0% | |
| ! | CGCGGCGTTGGAGAAACAGC+CGG | - | chr4.3:49531171-49531190 | MS.gene66619:CDS | 65.0% |
| CGTGAAGGATCGTGCGGCGG+AGG | - | chr4.3:49531455-49531474 | MS.gene66619:CDS | 70.0% | |
| GGGCACGGTTTGCGGGAACC+GGG | - | chr4.3:49531514-49531533 | MS.gene66619:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 49531117 | 49531569 | 49531117 | ID=MS.gene66619 |
| chr4.3 | mRNA | 49531117 | 49531569 | 49531117 | ID=MS.gene66619.t1;Parent=MS.gene66619 |
| chr4.3 | exon | 49531117 | 49531569 | 49531117 | ID=MS.gene66619.t1.exon1;Parent=MS.gene66619.t1 |
| chr4.3 | CDS | 49531117 | 49531569 | 49531117 | ID=cds.MS.gene66619.t1;Parent=MS.gene66619.t1 |
| Gene Sequence |
| Protein sequence |
