Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66709.t1 | XP_013455778.1 | 95.1 | 103 | 5 | 0 | 1 | 103 | 1 | 103 | 1.90E-43 | 184.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66709.t1 | A0A072UJ84 | 95.1 | 103 | 5 | 0 | 1 | 103 | 1 | 103 | 1.4e-43 | 184.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050100 | MS.gene66709 | 0.811964 | 5.38E-51 | -1.69E-46 |
| MS.gene055359 | MS.gene66709 | 0.816511 | 5.33E-52 | -1.69E-46 |
| MS.gene059632 | MS.gene66709 | 0.810092 | 1.37E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66709.t1 | MTR_4g052230 | 95.146 | 103 | 5 | 0 | 1 | 103 | 1 | 103 | 1.16e-54 | 165 |
| MS.gene66709.t1 | MTR_2g086870 | 58.974 | 78 | 28 | 2 | 28 | 103 | 1 | 76 | 1.08e-21 | 81.3 |
| MS.gene66709.t1 | MTR_7g105730 | 47.000 | 100 | 51 | 2 | 1 | 99 | 14 | 112 | 3.50e-20 | 78.6 |
| MS.gene66709.t1 | MTR_1g058120 | 41.379 | 116 | 51 | 4 | 1 | 101 | 1 | 114 | 4.58e-18 | 73.2 |
| MS.gene66709.t1 | MTR_2g086850 | 60.976 | 41 | 16 | 0 | 54 | 94 | 70 | 110 | 8.87e-13 | 59.7 |
| MS.gene66709.t1 | MTR_2g086860 | 41.379 | 87 | 40 | 4 | 13 | 90 | 6 | 90 | 9.47e-12 | 56.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66709.t1 | AT3G11600 | 46.809 | 94 | 45 | 2 | 1 | 90 | 1 | 93 | 2.36e-20 | 79.3 |
| MS.gene66709.t1 | AT5G06270 | 76.923 | 39 | 9 | 0 | 53 | 91 | 62 | 100 | 2.59e-16 | 68.9 |
| MS.gene66709.t1 | AT5G06270 | 76.923 | 39 | 9 | 0 | 53 | 91 | 62 | 100 | 2.59e-16 | 68.9 |
Find 24 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTCTTGCACTTGGGACATT+TGG | 0.291863 | 4.3:-47698934 | None:intergenic |
| GGACATTTGGGATCAACTTC+TGG | 0.305242 | 4.3:-47698921 | None:intergenic |
| CTCTTGCACTTGGGACATTT+GGG | 0.346227 | 4.3:-47698933 | None:intergenic |
| TTCAACGTTGGAAGTGGCAG+TGG | 0.382654 | 4.3:+47698728 | MS.gene66709:CDS |
| ACAAAAGCCATGCTTCTTGT+TGG | 0.427150 | 4.3:+47698867 | MS.gene66709:CDS |
| AGCGACGAGGTTGCCGCCCT+TGG | 0.428218 | 4.3:-47698768 | None:intergenic |
| TCGATGCCTCATGTATGTTA+TGG | 0.453292 | 4.3:+47698896 | MS.gene66709:CDS |
| CAACATAGTACTCTTGCACT+TGG | 0.472840 | 4.3:-47698943 | None:intergenic |
| GACGAGGTTGCCGCCCTTGG+TGG | 0.478335 | 4.3:-47698765 | None:intergenic |
| CCAACATGAGTAAAAGATCA+AGG | 0.518273 | 4.3:+47698703 | None:intergenic |
| GGCAACCTCGTCGCTATCAT+CGG | 0.522850 | 4.3:+47698776 | MS.gene66709:CDS |
| AAGGAGTTCAACGTTGGAAG+TGG | 0.536489 | 4.3:+47698722 | MS.gene66709:CDS |
| GCTGAAACTGTCTCCACCAA+GGG | 0.542317 | 4.3:+47698752 | MS.gene66709:CDS |
| AACATAGTACTCTTGCACTT+GGG | 0.557195 | 4.3:-47698942 | None:intergenic |
| TCAACGTTGGAAGTGGCAGT+GGG | 0.573815 | 4.3:+47698729 | MS.gene66709:CDS |
| GAATGTGTTCATTCGTCGGA+AGG | 0.586297 | 4.3:+47698813 | MS.gene66709:CDS |
| GACAGAATGTGTTCATTCGT+CGG | 0.592266 | 4.3:+47698809 | MS.gene66709:CDS |
| GGCTGAAACTGTCTCCACCA+AGG | 0.595762 | 4.3:+47698751 | MS.gene66709:CDS |
| AAGATCAAGGAGTTCAACGT+TGG | 0.606892 | 4.3:+47698716 | MS.gene66709:CDS |
| GAAACTGTCTCCACCAAGGG+CGG | 0.648786 | 4.3:+47698755 | MS.gene66709:CDS |
| AGTACAACCAACAAGAAGCA+TGG | 0.652627 | 4.3:-47698874 | None:intergenic |
| CTGGTACCATAACATACATG+AGG | 0.677492 | 4.3:-47698902 | None:intergenic |
| CAACGTTGGAAGTGGCAGTG+GGG | 0.707130 | 4.3:+47698730 | MS.gene66709:CDS |
| AGATTCCGATGATAGCGACG+AGG | 0.779553 | 4.3:-47698781 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACATAGTACTCTTGCACTT+GGG | - | chr4.3:47698945-47698964 | None:intergenic | 35.0% | |
| ! | GCTTGATTTTCTTAACAGTG+AGG | + | chr4.3:47698965-47698984 | MS.gene66709:CDS | 35.0% |
| AAGATCAAGGAGTTCAACGT+TGG | + | chr4.3:47698716-47698735 | MS.gene66709:CDS | 40.0% | |
| ACAAAAGCCATGCTTCTTGT+TGG | + | chr4.3:47698867-47698886 | MS.gene66709:CDS | 40.0% | |
| AGTACAACCAACAAGAAGCA+TGG | - | chr4.3:47698877-47698896 | None:intergenic | 40.0% | |
| CAACATAGTACTCTTGCACT+TGG | - | chr4.3:47698946-47698965 | None:intergenic | 40.0% | |
| CTGGTACCATAACATACATG+AGG | - | chr4.3:47698905-47698924 | None:intergenic | 40.0% | |
| GACAGAATGTGTTCATTCGT+CGG | + | chr4.3:47698809-47698828 | MS.gene66709:CDS | 40.0% | |
| TCGATGCCTCATGTATGTTA+TGG | + | chr4.3:47698896-47698915 | MS.gene66709:CDS | 40.0% | |
| AAGGAGTTCAACGTTGGAAG+TGG | + | chr4.3:47698722-47698741 | MS.gene66709:CDS | 45.0% | |
| ACTCTTGCACTTGGGACATT+TGG | - | chr4.3:47698937-47698956 | None:intergenic | 45.0% | |
| CTCTTGCACTTGGGACATTT+GGG | - | chr4.3:47698936-47698955 | None:intergenic | 45.0% | |
| GAATGTGTTCATTCGTCGGA+AGG | + | chr4.3:47698813-47698832 | MS.gene66709:CDS | 45.0% | |
| ! | GGACATTTGGGATCAACTTC+TGG | - | chr4.3:47698924-47698943 | None:intergenic | 45.0% |
| GCTGAAACTGTCTCCACCAA+GGG | + | chr4.3:47698752-47698771 | MS.gene66709:CDS | 50.0% | |
| TCAACGTTGGAAGTGGCAGT+GGG | + | chr4.3:47698729-47698748 | MS.gene66709:CDS | 50.0% | |
| TTCAACGTTGGAAGTGGCAG+TGG | + | chr4.3:47698728-47698747 | MS.gene66709:CDS | 50.0% | |
| ! | AGATTCCGATGATAGCGACG+AGG | - | chr4.3:47698784-47698803 | None:intergenic | 50.0% |
| CAACGTTGGAAGTGGCAGTG+GGG | + | chr4.3:47698730-47698749 | MS.gene66709:CDS | 55.0% | |
| GAAACTGTCTCCACCAAGGG+CGG | + | chr4.3:47698755-47698774 | MS.gene66709:CDS | 55.0% | |
| GGCAACCTCGTCGCTATCAT+CGG | + | chr4.3:47698776-47698795 | MS.gene66709:CDS | 55.0% | |
| GGCTGAAACTGTCTCCACCA+AGG | + | chr4.3:47698751-47698770 | MS.gene66709:CDS | 55.0% | |
| AGCGACGAGGTTGCCGCCCT+TGG | - | chr4.3:47698771-47698790 | None:intergenic | 70.0% | |
| GACGAGGTTGCCGCCCTTGG+TGG | - | chr4.3:47698768-47698787 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 47698708 | 47699019 | 47698708 | ID=MS.gene66709 |
| chr4.3 | mRNA | 47698708 | 47699019 | 47698708 | ID=MS.gene66709.t1;Parent=MS.gene66709 |
| chr4.3 | exon | 47698708 | 47699019 | 47698708 | ID=MS.gene66709.t1.exon1;Parent=MS.gene66709.t1 |
| chr4.3 | CDS | 47698708 | 47699019 | 47698708 | ID=cds.MS.gene66709.t1;Parent=MS.gene66709.t1 |
| Gene Sequence |
| Protein sequence |
