AlfalfaGEDB | MS.gene66771

M. sativa cultivar XinJiangDaYe / MS.gene66771

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0097159 GO:1901363
PFAM: PF01428.19 PF01754.19

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66771.t1	XP_003606132.1	85.3	170	21	1	1	166	1	170	4.40E-74	287.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66771.t1	Q9SJM6	48.8	168	77	2	1	166	1	161	4.3e-38	159.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66771.t1	G7JIG0	85.3	170	21	1	1	166	1	170	3.2e-74	287.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene24724	MS.gene66771	PPI
MS.gene050335	MS.gene66771	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66771.t1	MTR_4g053440	93.529	170	7	2	1	166	1	170	7.38e-114	320
MS.gene66771.t1	MTR_2g086190	56.627	166	56	3	1	166	1	150	9.79e-58	177
MS.gene66771.t1	MTR_7g104320	50.568	176	69	4	1	166	1	168	1.94e-56	175
MS.gene66771.t1	MTR_1g060380	50.877	171	64	4	1	166	1	156	5.64e-48	153
MS.gene66771.t1	MTR_2g098160	40.571	175	87	5	3	166	4	172	1.00e-40	135
MS.gene66771.t1	MTR_0249s0070	42.012	169	89	5	3	166	5	169	3.28e-39	131
MS.gene66771.t1	MTR_0249s0070	42.012	169	89	5	3	166	13	177	3.91e-39	131
MS.gene66771.t1	MTR_7g092400	39.011	182	79	5	3	166	93	260	9.81e-38	130
MS.gene66771.t1	MTR_7g114920	40.789	152	63	5	13	163	5	130	2.14e-31	110
MS.gene66771.t1	MTR_4g065570	37.931	145	75	3	28	166	2	137	6.89e-31	108
MS.gene66771.t1	MTR_1g100773	39.073	151	74	4	13	163	5	137	5.92e-30	106
MS.gene66771.t1	MTR_3g028010	33.766	154	84	2	13	166	28	163	1.85e-24	93.6
MS.gene66771.t1	MTR_8g036980	56.452	62	27	0	105	166	90	151	1.42e-22	88.2
MS.gene66771.t1	MTR_2g054650	38.158	152	60	7	13	163	6	124	9.41e-20	80.1
MS.gene66771.t1	MTR_3g025570	54.545	44	20	0	101	144	4	47	5.90e-12	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66771.t1	AT2G27580	56.024	166	70	2	1	166	1	163	8.96e-55	170
MS.gene66771.t1	AT2G27580	56.024	166	70	2	1	166	1	163	8.96e-55	170
MS.gene66771.t1	AT2G36320	48.810	168	77	2	1	166	1	161	1.13e-50	160
MS.gene66771.t1	AT1G12440	45.806	155	81	1	12	166	17	168	1.40e-45	147
MS.gene66771.t1	AT1G12440	45.806	155	81	1	12	166	17	168	1.40e-45	147
MS.gene66771.t1	AT1G12440	45.806	155	81	1	12	166	17	168	1.40e-45	147
MS.gene66771.t1	AT3G52800	47.701	174	79	3	1	166	1	170	3.99e-42	139
MS.gene66771.t1	AT3G52800	47.701	174	79	3	1	166	1	170	3.99e-42	139
MS.gene66771.t1	AT4G12040	43.529	170	70	4	12	166	17	175	4.78e-42	139
MS.gene66771.t1	AT4G12040	43.529	170	70	4	12	166	17	175	4.78e-42	139
MS.gene66771.t1	AT4G22820	44.099	161	80	4	12	166	20	176	1.02e-41	137
MS.gene66771.t1	AT4G22820	44.099	161	80	4	12	166	20	176	1.02e-41	137
MS.gene66771.t1	AT1G51200	44.099	161	81	4	12	166	16	173	1.18e-37	127
MS.gene66771.t1	AT1G51200	44.099	161	81	4	12	166	16	173	1.18e-37	127
MS.gene66771.t1	AT1G51200	44.099	161	81	4	12	166	16	173	1.18e-37	127
MS.gene66771.t1	AT1G51200	44.099	161	81	4	12	166	16	173	1.18e-37	127
MS.gene66771.t1	AT3G12630	34.211	152	74	4	19	166	31	160	1.55e-24	93.6
MS.gene66771.t1	AT4G25380	34.641	153	68	6	14	166	10	130	3.81e-23	89.0
MS.gene66771.t1	AT4G14225	31.613	155	69	2	13	166	7	125	2.41e-20	81.6

Find 54 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACGTTCTTCATCTGCAAAAT+TGG	0.253435	4.3:+46734327	MS.gene66771:CDS
CTGCATGTCCCACACCTATT+TGG	0.276997	4.3:-46734511	None:intergenic
GCTAACAACTGCGGTTTCTT+TGG	0.331458	4.3:+46734244	MS.gene66771:CDS
CACCTATTTGGCTGGACCTT+CGG	0.347652	4.3:-46734499	None:intergenic
CTGCAAATCACGGTAACATT+TGG	0.369389	4.3:-46734293	None:intergenic
AGCTTCTCTCCCTTCACCAC+TGG	0.384998	4.3:-46734667	None:intergenic
TATCCGGAGCAGCATGGTTG+TGG	0.393608	4.3:+46734598	MS.gene66771:CDS
GTGGAGTTGGTAGCTGCTGC+TGG	0.406487	4.3:+46734457	MS.gene66771:CDS
CACCGAAGGTCCAGCCAAAT+AGG	0.408383	4.3:+46734497	MS.gene66771:CDS
TGGGACATGCAGACGGCGCG+TGG	0.435856	4.3:+46734522	MS.gene66771:CDS
GTGTGGGACCCACAGGTATC+CGG	0.436288	4.3:+46734582	MS.gene66771:CDS
ACTGTTGAGGAATCAACGTT+TGG	0.447132	4.3:-46734357	None:intergenic
GAGCAAAGATCTTGCATGGC+AGG	0.449620	4.3:-46734271	None:intergenic
GGTAGCTGCTGCTGGACCTT+CGG	0.453896	4.3:+46734465	MS.gene66771:CDS
AGACGGCGCGTGGGGCTGAC+AGG	0.473736	4.3:+46734532	MS.gene66771:CDS
ATGTCCCACACCTATTTGGC+TGG	0.481879	4.3:-46734507	None:intergenic
AACCCACAACCATGCTGCTC+CGG	0.487234	4.3:-46734601	None:intergenic
CACAGAGACGTTGAGCAGCT+TGG	0.496369	4.3:-46734222	None:intergenic
ATCCGGAGCAGCATGGTTGT+GGG	0.508507	4.3:+46734599	MS.gene66771:CDS
TTACCGTGATTTGCAGATGA+AGG	0.514621	4.3:+46734300	MS.gene66771:CDS
TGACGTTGTGTGGGACCCAC+AGG	0.525772	4.3:+46734575	MS.gene66771:CDS
ACTACAACCGACGGTGGAGA+TGG	0.535288	4.3:-46734415	None:intergenic
GAAGGCGAATCCAGTGGTGA+AGG	0.546816	4.3:+46734657	MS.gene66771:CDS
ATCTACACCGACGGTGGAGT+TGG	0.547793	4.3:+46734444	MS.gene66771:CDS
TTCGGTGGTTCAGCCTCCGA+AGG	0.562954	4.3:-46734481	None:intergenic
CAAGAAGAATTAGACTGTTG+AGG	0.566912	4.3:-46734370	None:intergenic
GGCTGACAGGATTTAAGTGT+CGG	0.567077	4.3:+46734545	MS.gene66771:CDS
CATGCTGCTCCGGATACCTG+TGG	0.567299	4.3:-46734591	None:intergenic
CTATTTGGCTGGACCTTCGG+TGG	0.568326	4.3:-46734496	None:intergenic
AGCTGCTGCTGGACCTTCGG+AGG	0.570808	4.3:+46734468	MS.gene66771:CDS
TTTGGAGCAAAGATCTTGCA+TGG	0.576644	4.3:-46734275	None:intergenic
GGGACATGCAGACGGCGCGT+GGG	0.582538	4.3:+46734523	MS.gene66771:CDS
CGGTGCGGATTGACGTTGTG+TGG	0.586129	4.3:+46734565	MS.gene66771:CDS
GGACATGCAGACGGCGCGTG+GGG	0.594994	4.3:+46734524	MS.gene66771:CDS
GTTCCTTCATCTGCAAATCA+CGG	0.602813	4.3:-46734303	None:intergenic
ATGCTGCTCCGGATACCTGT+GGG	0.605743	4.3:-46734590	None:intergenic
AAGGTCCAGCCAAATAGGTG+TGG	0.606672	4.3:+46734502	MS.gene66771:CDS
TATTCATCCATCTCCACCGT+CGG	0.610000	4.3:+46734408	MS.gene66771:CDS
AAGGCGAATCCAGTGGTGAA+GGG	0.610190	4.3:+46734658	MS.gene66771:CDS
AGGTCCAGCCAAATAGGTGT+GGG	0.616831	4.3:+46734503	MS.gene66771:CDS
TTCGGAGGCTGAACCACCGA+AGG	0.620244	4.3:+46734483	MS.gene66771:CDS
TAATTCTTCTTGTCTAGACA+CGG	0.623144	4.3:+46734381	MS.gene66771:CDS
GGTGCGGATTGACGTTGTGT+GGG	0.623964	4.3:+46734566	MS.gene66771:CDS
GCAGCTACCAACTCCACCGT+CGG	0.632431	4.3:-46734451	None:intergenic
ATAGGTGTGGGACATGCAGA+CGG	0.635820	4.3:+46734515	MS.gene66771:CDS
AGTTTCTTCATCTACACCGA+CGG	0.637393	4.3:+46734435	MS.gene66771:CDS
CACAGGTATCCGGAGCAGCA+TGG	0.641422	4.3:+46734592	MS.gene66771:CDS
TTCTTCATCTACACCGACGG+TGG	0.645268	4.3:+46734438	MS.gene66771:CDS
GGGAAGGGAGGAGATCAAGA+AGG	0.649649	4.3:+46734639	MS.gene66771:CDS
GATCAAGAAGGCGAATCCAG+TGG	0.675101	4.3:+46734651	MS.gene66771:CDS
ACAGGATTTAAGTGTCGGTG+CGG	0.676149	4.3:+46734550	MS.gene66771:CDS
GATGAAGAAACTACAACCGA+CGG	0.679990	4.3:-46734424	None:intergenic
CGTCTCTGTGCTAACAACTG+CGG	0.696402	4.3:+46734235	MS.gene66771:CDS
GAAGAAACTACAACCGACGG+TGG	0.716652	4.3:-46734421	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TAATTCTTCTTGTCTAGACA+CGG	+	chr4.3:46734381-46734400	MS.gene66771:CDS	30.0%
	ACGTTCTTCATCTGCAAAAT+TGG	+	chr4.3:46734327-46734346	MS.gene66771:CDS	35.0%
	CAAGAAGAATTAGACTGTTG+AGG	-	chr4.3:46734373-46734392	None:intergenic	35.0%
!!	GGTTGTGGGTTTGATTTTAA+GGG	+	chr4.3:46734613-46734632	MS.gene66771:CDS	35.0%
!!	GTTGTGGGTTTGATTTTAAG+GGG	+	chr4.3:46734614-46734633	MS.gene66771:CDS	35.0%
!!	TGGTTGTGGGTTTGATTTTA+AGG	+	chr4.3:46734612-46734631	MS.gene66771:CDS	35.0%
	ACTGTTGAGGAATCAACGTT+TGG	-	chr4.3:46734360-46734379	None:intergenic	40.0%
	AGTTTCTTCATCTACACCGA+CGG	+	chr4.3:46734435-46734454	MS.gene66771:CDS	40.0%
	CTGCAAATCACGGTAACATT+TGG	-	chr4.3:46734296-46734315	None:intergenic	40.0%
	GATGAAGAAACTACAACCGA+CGG	-	chr4.3:46734427-46734446	None:intergenic	40.0%
	GTTCCTTCATCTGCAAATCA+CGG	-	chr4.3:46734306-46734325	None:intergenic	40.0%
!	TTACCGTGATTTGCAGATGA+AGG	+	chr4.3:46734300-46734319	MS.gene66771:CDS	40.0%
!	TTTGGAGCAAAGATCTTGCA+TGG	-	chr4.3:46734278-46734297	None:intergenic	40.0%
!!	GGGTTTGATTTTAAGGGGAT+GGG	+	chr4.3:46734619-46734638	MS.gene66771:CDS	40.0%
!!	TGATTTTAAGGGGATGGGAA+GGG	+	chr4.3:46734624-46734643	MS.gene66771:CDS	40.0%
!!	TGGGTTTGATTTTAAGGGGA+TGG	+	chr4.3:46734618-46734637	MS.gene66771:CDS	40.0%
!!	TTGATTTTAAGGGGATGGGA+AGG	+	chr4.3:46734623-46734642	MS.gene66771:CDS	40.0%
	GCTAACAACTGCGGTTTCTT+TGG	+	chr4.3:46734244-46734263	MS.gene66771:CDS	45.0%
	GGCTGACAGGATTTAAGTGT+CGG	+	chr4.3:46734545-46734564	MS.gene66771:CDS	45.0%
	TATTCATCCATCTCCACCGT+CGG	+	chr4.3:46734408-46734427	MS.gene66771:CDS	45.0%
!!	ACAGGATTTAAGTGTCGGTG+CGG	+	chr4.3:46734550-46734569	MS.gene66771:CDS	45.0%
	AAGGCGAATCCAGTGGTGAA+GGG	+	chr4.3:46734658-46734677	MS.gene66771:CDS	50.0%
	AAGGTCCAGCCAAATAGGTG+TGG	+	chr4.3:46734502-46734521	MS.gene66771:CDS	50.0%
	AGGTCCAGCCAAATAGGTGT+GGG	+	chr4.3:46734503-46734522	MS.gene66771:CDS	50.0%
	ATAGGTGTGGGACATGCAGA+CGG	+	chr4.3:46734515-46734534	MS.gene66771:CDS	50.0%
	ATGTCCCACACCTATTTGGC+TGG	-	chr4.3:46734510-46734529	None:intergenic	50.0%
	CACCTATTTGGCTGGACCTT+CGG	-	chr4.3:46734502-46734521	None:intergenic	50.0%
	CGTCTCTGTGCTAACAACTG+CGG	+	chr4.3:46734235-46734254	MS.gene66771:CDS	50.0%
	CTGCATGTCCCACACCTATT+TGG	-	chr4.3:46734514-46734533	None:intergenic	50.0%
	GAAGAAACTACAACCGACGG+TGG	-	chr4.3:46734424-46734443	None:intergenic	50.0%
	GAGCAAAGATCTTGCATGGC+AGG	-	chr4.3:46734274-46734293	None:intergenic	50.0%
	GATCAAGAAGGCGAATCCAG+TGG	+	chr4.3:46734651-46734670	MS.gene66771:CDS	50.0%
	TTCTTCATCTACACCGACGG+TGG	+	chr4.3:46734438-46734457	MS.gene66771:CDS	50.0%
!!	TTTTAAGGGGATGGGAAGGG+AGG	+	chr4.3:46734627-46734646	MS.gene66771:CDS	50.0%
	AACCCACAACCATGCTGCTC+CGG	-	chr4.3:46734604-46734623	None:intergenic	55.0%
	ACTACAACCGACGGTGGAGA+TGG	-	chr4.3:46734418-46734437	None:intergenic	55.0%
	AGCTTCTCTCCCTTCACCAC+TGG	-	chr4.3:46734670-46734689	None:intergenic	55.0%
	ATCCGGAGCAGCATGGTTGT+GGG	+	chr4.3:46734599-46734618	MS.gene66771:CDS	55.0%
	ATCTACACCGACGGTGGAGT+TGG	+	chr4.3:46734444-46734463	MS.gene66771:CDS	55.0%
	ATGCTGCTCCGGATACCTGT+GGG	-	chr4.3:46734593-46734612	None:intergenic	55.0%
	CACAGAGACGTTGAGCAGCT+TGG	-	chr4.3:46734225-46734244	None:intergenic	55.0%
	CACCGAAGGTCCAGCCAAAT+AGG	+	chr4.3:46734497-46734516	MS.gene66771:CDS	55.0%
	CTATTTGGCTGGACCTTCGG+TGG	-	chr4.3:46734499-46734518	None:intergenic	55.0%
	GAAGGCGAATCCAGTGGTGA+AGG	+	chr4.3:46734657-46734676	MS.gene66771:CDS	55.0%
	GGGAAGGGAGGAGATCAAGA+AGG	+	chr4.3:46734639-46734658	MS.gene66771:CDS	55.0%
	TATCCGGAGCAGCATGGTTG+TGG	+	chr4.3:46734598-46734617	MS.gene66771:CDS	55.0%
!	GGTGCGGATTGACGTTGTGT+GGG	+	chr4.3:46734566-46734585	MS.gene66771:CDS	55.0%
	CACAGGTATCCGGAGCAGCA+TGG	+	chr4.3:46734592-46734611	MS.gene66771:CDS	60.0%
	CATGCTGCTCCGGATACCTG+TGG	-	chr4.3:46734594-46734613	None:intergenic	60.0%
	GCAGCTACCAACTCCACCGT+CGG	-	chr4.3:46734454-46734473	None:intergenic	60.0%
	GGTAGCTGCTGCTGGACCTT+CGG	+	chr4.3:46734465-46734484	MS.gene66771:CDS	60.0%
	GTGTGGGACCCACAGGTATC+CGG	+	chr4.3:46734582-46734601	MS.gene66771:CDS	60.0%
	TGACGTTGTGTGGGACCCAC+AGG	+	chr4.3:46734575-46734594	MS.gene66771:CDS	60.0%
	TTCGGAGGCTGAACCACCGA+AGG	+	chr4.3:46734483-46734502	MS.gene66771:CDS	60.0%
	TTCGGTGGTTCAGCCTCCGA+AGG	-	chr4.3:46734484-46734503	None:intergenic	60.0%
!	CGGTGCGGATTGACGTTGTG+TGG	+	chr4.3:46734565-46734584	MS.gene66771:CDS	60.0%
!!	GTGGAGTTGGTAGCTGCTGC+TGG	+	chr4.3:46734457-46734476	MS.gene66771:CDS	60.0%
	AGCTGCTGCTGGACCTTCGG+AGG	+	chr4.3:46734468-46734487	MS.gene66771:CDS	65.0%
	GGACATGCAGACGGCGCGTG+GGG	+	chr4.3:46734524-46734543	MS.gene66771:CDS	70.0%
	GGGACATGCAGACGGCGCGT+GGG	+	chr4.3:46734523-46734542	MS.gene66771:CDS	70.0%
	TGGGACATGCAGACGGCGCG+TGG	+	chr4.3:46734522-46734541	MS.gene66771:CDS	70.0%
	AGACGGCGCGTGGGGCTGAC+AGG	+	chr4.3:46734532-46734551	MS.gene66771:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	46734202	46734702	46734202	ID=MS.gene66771
chr4.3	mRNA	46734202	46734702	46734202	ID=MS.gene66771.t1;Parent=MS.gene66771
chr4.3	exon	46734202	46734702	46734202	ID=MS.gene66771.t1.exon1;Parent=MS.gene66771.t1
chr4.3	CDS	46734202	46734702	46734202	ID=cds.MS.gene66771.t1;Parent=MS.gene66771.t1

Gene Sequence

>MS.gene66771

ATGGCTGAAGAACAAAGATGCCAAGCTGCTCAACGTCTCTGTGCTAACAACTGCGGTTTCTTTGGAAGTCCTGCCATGCAAGATCTTTGCTCCAAATGTTACCGTGATTTGCAGATGAAGGAACAACGTTCTTCATCTGCAAAATTGGTTTTAAACCAAACGTTGATTCCTCAACAGTCTAATTCTTCTTGTCTAGACACGGAGATTATTCATCCATCTCCACCGTCGGTTGTAGTTTCTTCATCTACACCGACGGTGGAGTTGGTAGCTGCTGCTGGACCTTCGGAGGCTGAACCACCGAAGGTCCAGCCAAATAGGTGTGGGACATGCAGACGGCGCGTGGGGCTGACAGGATTTAAGTGTCGGTGCGGATTGACGTTGTGTGGGACCCACAGGTATCCGGAGCAGCATGGTTGTGGGTTTGATTTTAAGGGGATGGGAAGGGAGGAGATCAAGAAGGCGAATCCAGTGGTGAAGGGAGAGAAGCTGAATAAGATCTGA

Protein sequence

>MS.gene66771.t1

MAEEQRCQAAQRLCANNCGFFGSPAMQDLCSKCYRDLQMKEQRSSSAKLVLNQTLIPQQSNSSCLDTEIIHPSPPSVVVSSSTPTVELVAAAGPSEAEPPKVQPNRCGTCRRRVGLTGFKCRCGLTLCGTHRYPEQHGCGFDFKGMGREEIKKANPVVKGEKLNKI