Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66806.t1 | GAU41437.1 | 92.1 | 101 | 8 | 0 | 1 | 101 | 66 | 166 | 2.10E-47 | 198 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66806.t1 | Q55535 | 46.1 | 89 | 40 | 2 | 1 | 89 | 54 | 134 | 1.3e-13 | 77.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66806.t1 | A0A2Z6PCX8 | 92.1 | 101 | 8 | 0 | 1 | 101 | 66 | 166 | 1.5e-47 | 198.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052253 | MS.gene66806 | 0.817119 | 3.89E-52 | -1.69E-46 |
| MS.gene059285 | MS.gene66806 | 0.803703 | 3.07E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66806.t1 | MTR_4g055030 | 96.667 | 90 | 3 | 0 | 1 | 90 | 67 | 156 | 1.17e-62 | 188 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66806.t1 | AT3G44620 | 75.556 | 90 | 22 | 0 | 1 | 90 | 130 | 219 | 2.06e-44 | 144 |
| MS.gene66806.t1 | AT3G44620 | 60.177 | 113 | 22 | 1 | 1 | 90 | 130 | 242 | 6.48e-39 | 130 |
Find 17 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCTGAAGATGCACACAAAA+AGG | 0.322490 | 4.3:-46178924 | MS.gene66806:intron |
| ACAAAATCAGAAGGCTTGAT+TGG | 0.384101 | 4.3:+46179137 | None:intergenic |
| TTCAGATAACTTCAATTTCA+AGG | 0.407069 | 4.3:-46179160 | MS.gene66806:CDS |
| TTGTGTGCATCTTCAGGTAA+AGG | 0.430130 | 4.3:+46178929 | None:intergenic |
| GTTTGATCTTATTCTTGCTA+TGG | 0.444378 | 4.3:-46179111 | MS.gene66806:CDS |
| GAAGTCCCGGATCCTTACTA+TGG | 0.472238 | 4.3:-46178460 | MS.gene66806:CDS |
| GGTCCACCATAGTAAGGATC+CGG | 0.483919 | 4.3:+46178454 | None:intergenic |
| CCTTGAGGTCCACCATAGTA+AGG | 0.486542 | 4.3:+46178448 | None:intergenic |
| CCTTACTATGGTGGACCTCA+AGG | 0.500453 | 4.3:-46178448 | MS.gene66806:CDS |
| GCATGATGAAACCGAAGTCC+CGG | 0.534176 | 4.3:-46178473 | MS.gene66806:CDS |
| ATTCAAGACTATCTTTGCAT+CGG | 0.567065 | 4.3:+46178344 | None:intergenic |
| ATAGTAAGGATCCGGGACTT+CGG | 0.579621 | 4.3:+46178462 | None:intergenic |
| GTCCACCATAGTAAGGATCC+GGG | 0.586473 | 4.3:+46178455 | None:intergenic |
| TCAAACTCAACAAAATCAGA+AGG | 0.591117 | 4.3:+46179128 | None:intergenic |
| GTCCCGGATCCTTACTATGG+TGG | 0.618032 | 4.3:-46178457 | MS.gene66806:CDS |
| GCTATGGACAAGCAAAACAA+AGG | 0.649274 | 4.3:-46179095 | MS.gene66806:intron |
| TATACCTTCTCAAAACCTTG+AGG | 0.655564 | 4.3:+46178433 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATAAAATTTTCATTTTTCCT+TGG | + | chr4.3:46178835-46178854 | None:intergenic | 15.0% |
| !!! | CTCTTTTTATTAAATTGTAA+TGG | - | chr4.3:46178521-46178540 | MS.gene66806:intron | 15.0% |
| !! | AAAACAAAAAAACAACAACA+AGG | + | chr4.3:46178548-46178567 | None:intergenic | 20.0% |
| !! | TATTCTTTATGATAGGTTAT+TGG | - | chr4.3:46179128-46179147 | MS.gene66806:CDS | 20.0% |
| !!! | AGTTTTTTTTTTTCTCTTTC+TGG | - | chr4.3:46178873-46178892 | MS.gene66806:intron | 20.0% |
| !!! | TTGTTGTTTTTTTGTTTTAG+AGG | - | chr4.3:46178550-46178569 | MS.gene66806:intron | 20.0% |
| ! | AATCTGAATTTGGTTGTTTA+TGG | - | chr4.3:46178486-46178505 | MS.gene66806:CDS | 25.0% |
| ! | GAGATGTTATAATCTGAATT+TGG | - | chr4.3:46178476-46178495 | MS.gene66806:CDS | 25.0% |
| ! | TTCAGATAACTTCAATTTCA+AGG | - | chr4.3:46178380-46178399 | MS.gene66806:intron | 25.0% |
| ! | TTGTTAGACAACCTAAATTA+AGG | - | chr4.3:46178914-46178933 | MS.gene66806:intron | 25.0% |
| !! | GAAGGTATATTCTTTATGAT+AGG | - | chr4.3:46179121-46179140 | MS.gene66806:CDS | 25.0% |
| !! | TTGTTCTAAATCGTATATTG+TGG | - | chr4.3:46178759-46178778 | MS.gene66806:intron | 25.0% |
| !!! | TTTTGTTTTAGAGGACATAT+TGG | - | chr4.3:46178559-46178578 | MS.gene66806:intron | 25.0% |
| !!! | TTTTTTTTTCTCTTTCTGGT+AGG | - | chr4.3:46178877-46178896 | MS.gene66806:intron | 25.0% |
| CGTATATTGTGGAACAATTA+TGG | - | chr4.3:46178770-46178789 | MS.gene66806:intron | 30.0% | |
| GTTTGATCTTATTCTTGCTA+TGG | - | chr4.3:46178429-46178448 | MS.gene66806:intron | 30.0% | |
| TCAAACTCAACAAAATCAGA+AGG | + | chr4.3:46178415-46178434 | None:intergenic | 30.0% | |
| TTTGACAACATTGAATTCCA+AGG | - | chr4.3:46178815-46178834 | MS.gene66806:intron | 30.0% | |
| ! | GTCTAACAAAAACTTGCTTT+AGG | + | chr4.3:46178903-46178922 | None:intergenic | 30.0% |
| ! | TATTGGAAGCTTTTAGTAGA+TGG | - | chr4.3:46178576-46178595 | MS.gene66806:intron | 30.0% |
| !! | AATATGTTTTTCAACCCTGA+TGG | - | chr4.3:46178996-46179015 | MS.gene66806:intron | 30.0% |
| ACAAAATCAGAAGGCTTGAT+TGG | + | chr4.3:46178406-46178425 | None:intergenic | 35.0% | |
| AGCAACAAAAGTTCTACTCA+CGG | + | chr4.3:46178967-46178986 | None:intergenic | 35.0% | |
| TATACCTTCTCAAAACCTTG+AGG | + | chr4.3:46179110-46179129 | None:intergenic | 35.0% | |
| TCACGGTCAACACAAATATA+AGG | + | chr4.3:46178950-46178969 | None:intergenic | 35.0% | |
| AAATCGAACCCCTTTATCCA+AGG | - | chr4.3:46178656-46178675 | MS.gene66806:intron | 40.0% | |
| ACAACACTGCCTTGGATAAA+GGG | + | chr4.3:46178668-46178687 | None:intergenic | 40.0% | |
| ACCCTTGGTTCATCTTTGTA+GGG | - | chr4.3:46179154-46179173 | MS.gene66806:CDS | 40.0% | |
| ACCTGAAGATGCACACAAAA+AGG | - | chr4.3:46178616-46178635 | MS.gene66806:intron | 40.0% | |
| CGGTCAACACAAATATAAGG+CGG | + | chr4.3:46178947-46178966 | None:intergenic | 40.0% | |
| GACAAGCAAAACAAAGGTAG+GGG | - | chr4.3:46178451-46178470 | MS.gene66806:CDS | 40.0% | |
| GCGGTTCAAGTCCTTAATTT+AGG | + | chr4.3:46178928-46178947 | None:intergenic | 40.0% | |
| GCTACAACATTCTAGCGTAT+CGG | + | chr4.3:46178722-46178741 | None:intergenic | 40.0% | |
| GCTATGGACAAGCAAAACAA+AGG | - | chr4.3:46178445-46178464 | MS.gene66806:CDS | 40.0% | |
| GGACAAGCAAAACAAAGGTA+GGG | - | chr4.3:46178450-46178469 | MS.gene66806:CDS | 40.0% | |
| TCCCTACAAAGATGAACCAA+GGG | + | chr4.3:46179158-46179177 | None:intergenic | 40.0% | |
| TGGACAAGCAAAACAAAGGT+AGG | - | chr4.3:46178449-46178468 | MS.gene66806:CDS | 40.0% | |
| TTGTCAATTGCGGACAATAG+CGG | - | chr4.3:46178684-46178703 | MS.gene66806:intron | 40.0% | |
| TTGTGTGCATCTTCAGGTAA+AGG | + | chr4.3:46178614-46178633 | None:intergenic | 40.0% | |
| ! | ACCTTTTTGTGTGCATCTTC+AGG | + | chr4.3:46178620-46178639 | None:intergenic | 40.0% |
| !! | ATAGGTTATTGGTTCACCCT+TGG | - | chr4.3:46179139-46179158 | MS.gene66806:CDS | 40.0% |
| ATAGTAAGGATCCGGGACTT+CGG | + | chr4.3:46179081-46179100 | None:intergenic | 45.0% | |
| CAACACTGCCTTGGATAAAG+GGG | + | chr4.3:46178667-46178686 | None:intergenic | 45.0% | |
| CAAGGCAGTGTTGTCAATTG+CGG | - | chr4.3:46178674-46178693 | MS.gene66806:intron | 45.0% | |
| CACCCTTGGTTCATCTTTGT+AGG | - | chr4.3:46179153-46179172 | MS.gene66806:CDS | 45.0% | |
| CTCCCTACAAAGATGAACCA+AGG | + | chr4.3:46179159-46179178 | None:intergenic | 45.0% | |
| GACAACACTGCCTTGGATAA+AGG | + | chr4.3:46178669-46178688 | None:intergenic | 45.0% | |
| TCAACCCTGATGGAGAGTTT+GGG | - | chr4.3:46179006-46179025 | MS.gene66806:intron | 45.0% | |
| TTCAACCCTGATGGAGAGTT+TGG | - | chr4.3:46179005-46179024 | MS.gene66806:intron | 45.0% | |
| !!! | TGGACCTCAAGGTTTTGAGA+AGG | - | chr4.3:46179103-46179122 | MS.gene66806:CDS | 45.0% |
| ACCATCCCAAACTCTCCATC+AGG | + | chr4.3:46179014-46179033 | None:intergenic | 50.0% | |
| AGGGCAGCTTCAAAAAGACG+TGG | - | chr4.3:46178355-46178374 | MS.gene66806:CDS | 50.0% | |
| CCATCCCAAACTCTCCATCA+GGG | + | chr4.3:46179013-46179032 | None:intergenic | 50.0% | |
| CCTTGAGGTCCACCATAGTA+AGG | + | chr4.3:46179095-46179114 | None:intergenic | 50.0% | |
| CGCAATTGACAACACTGCCT+TGG | + | chr4.3:46178676-46178695 | None:intergenic | 50.0% | |
| GAAGTCCCGGATCCTTACTA+TGG | - | chr4.3:46179080-46179099 | MS.gene66806:intron | 50.0% | |
| GCATGATGAAACCGAAGTCC+CGG | - | chr4.3:46179067-46179086 | MS.gene66806:intron | 50.0% | |
| GGGCAGCTTCAAAAAGACGT+GGG | - | chr4.3:46178356-46178375 | MS.gene66806:CDS | 50.0% | |
| GGTCCACCATAGTAAGGATC+CGG | + | chr4.3:46179089-46179108 | None:intergenic | 50.0% | |
| GTCCACCATAGTAAGGATCC+GGG | + | chr4.3:46179088-46179107 | None:intergenic | 50.0% | |
| !! | CCTTACTATGGTGGACCTCA+AGG | - | chr4.3:46179092-46179111 | MS.gene66806:intron | 50.0% |
| CCCTGATGGAGAGTTTGGGA+TGG | - | chr4.3:46179010-46179029 | MS.gene66806:intron | 55.0% | |
| GTCCCGGATCCTTACTATGG+TGG | - | chr4.3:46179083-46179102 | MS.gene66806:intron | 55.0% | |
| ! | TGGAGAGTTTGGGATGGTGC+AGG | - | chr4.3:46179016-46179035 | MS.gene66806:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 46178352 | 46179210 | 46178352 | ID=MS.gene66806 |
| chr4.3 | mRNA | 46178352 | 46179210 | 46178352 | ID=MS.gene66806.t1;Parent=MS.gene66806 |
| chr4.3 | exon | 46179096 | 46179210 | 46179096 | ID=MS.gene66806.t1.exon1;Parent=MS.gene66806.t1 |
| chr4.3 | CDS | 46179096 | 46179210 | 46179096 | ID=cds.MS.gene66806.t1;Parent=MS.gene66806.t1 |
| chr4.3 | exon | 46178925 | 46178992 | 46178925 | ID=MS.gene66806.t1.exon2;Parent=MS.gene66806.t1 |
| chr4.3 | CDS | 46178925 | 46178992 | 46178925 | ID=cds.MS.gene66806.t1;Parent=MS.gene66806.t1 |
| chr4.3 | exon | 46178438 | 46178524 | 46178438 | ID=MS.gene66806.t1.exon3;Parent=MS.gene66806.t1 |
| chr4.3 | CDS | 46178438 | 46178524 | 46178438 | ID=cds.MS.gene66806.t1;Parent=MS.gene66806.t1 |
| chr4.3 | exon | 46178352 | 46178387 | 46178352 | ID=MS.gene66806.t1.exon4;Parent=MS.gene66806.t1 |
| chr4.3 | CDS | 46178352 | 46178387 | 46178352 | ID=cds.MS.gene66806.t1;Parent=MS.gene66806.t1 |
| Gene Sequence |
| Protein sequence |
