AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene67121

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67121.t1 MTR_1g019620 94.366 355 3 1 1 338 1 355 0.0 671
MS.gene67121.t1 MTR_1g016110 93.803 355 5 1 1 338 1 355 0.0 665
MS.gene67121.t1 MTR_1g016110 98.377 308 5 0 31 338 1 308 0.0 614
MS.gene67121.t1 MTR_1g070360 34.228 298 144 10 37 326 17 270 5.14e-41 145
MS.gene67121.t1 MTR_6g086600 38.889 180 105 3 38 217 173 347 3.03e-29 117
MS.gene67121.t1 MTR_6g086600 38.889 180 105 3 38 217 164 338 3.17e-29 117
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67121.t1 AT5G50960 72.779 349 77 2 1 331 1 349 0.0 519
MS.gene67121.t1 AT4G19540 42.487 193 101 5 37 226 38 223 7.23e-37 135
MS.gene67121.t1 AT3G24430 38.333 180 106 3 38 217 172 346 2.77e-29 118

Find 86 sgRNAs with CRISPR-Local

Find 171 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCGATTAAATTCAACATTTC+AGG 0.198831 1.3:-3974545 None:intergenic
CCTTCTAGGCCAAGCATCTT+TGG 0.224706 1.3:-3974044 None:intergenic
GGTGTATCTCTTGACCTTCT+AGG 0.281189 1.3:-3974058 None:intergenic
GTCTATGTTGAGTCTAATCT+TGG 0.283572 1.3:+3974104 MS.gene67121:CDS
AATCTTGGGGTCATGTCAAT+TGG 0.295181 1.3:+3974119 MS.gene67121:CDS
ATCCAGACGAAGCTGTTATA+TGG 0.325193 1.3:+3974156 MS.gene67121:CDS
TTTATGAAGATTACAGATAA+TGG 0.328286 1.3:+3974470 MS.gene67121:CDS
CTTCTAGGCCAAGCATCTTT+GGG 0.359162 1.3:-3974043 None:intergenic
CTAATTGTTGATGCTCCGCC+TGG 0.377996 1.3:+3974248 MS.gene67121:CDS
CAGCTAACGCAAATGCCAGC+TGG 0.379548 1.3:-3973968 None:intergenic
CCAAAGATGCTTGGCCTAGA+AGG 0.402010 1.3:+3974044 MS.gene67121:CDS
CCAAGCATCTTTGGGATGCT+TGG 0.406720 1.3:-3974035 None:intergenic
AAAGTCGGAGTGAAAGTTCT+TGG 0.411803 1.3:+3974398 MS.gene67121:CDS
TCTATGTTGAGTCTAATCTT+GGG 0.416626 1.3:+3974105 MS.gene67121:CDS
CACTGTGAAACATAAGATAC+TGG 0.420840 1.3:+3973902 MS.gene67121:CDS
AAGTCGGAGTGAAAGTTCTT+GGG 0.427598 1.3:+3974399 MS.gene67121:CDS
ATCTTGGGGTCATGTCAATT+GGG 0.431936 1.3:+3974120 MS.gene67121:CDS
CATAAGATACTGGTCTTGTC+AGG 0.432544 1.3:+3973912 MS.gene67121:CDS
TAAAGGAACCTTACCGAGAA+AGG 0.434619 1.3:-3974633 None:intergenic
ACTTGAGATTTGAGATGGGC+TGG 0.443213 1.3:-3974448 None:intergenic
AGCTAACGCAAATGCCAGCT+GGG 0.447107 1.3:-3973967 None:intergenic
GCAGAATCAGACTGAGGACC+TGG 0.452269 1.3:-3972727 None:intergenic
CTCCATATAACAGCTTCGTC+TGG 0.454307 1.3:-3974158 None:intergenic
CATAAACTTGAGATTTGAGA+TGG 0.455458 1.3:-3974453 None:intergenic
AGTGAAAGTTCTTGGGGTTG+TGG 0.458548 1.3:+3974406 MS.gene67121:CDS
CGTCTACATTAGCAGCATCT+AGG 0.458881 1.3:-3974304 None:intergenic
CTTTGCAAGGCAGCTGAAGA+AGG 0.475604 1.3:+3974665 MS.gene67121:CDS
GGCGGTACCCTTTCTCGGTA+AGG 0.476321 1.3:+3974625 MS.gene67121:CDS
CTTCTGGGCATTGATATTTG+TGG 0.483404 1.3:+3974011 MS.gene67121:CDS
GAGATGGCGGTACCCTTTCT+CGG 0.483462 1.3:+3974620 MS.gene67121:CDS
TGGTGAGATGAAAGATGTTA+CGG 0.483466 1.3:+3974490 MS.gene67121:CDS
GGTCCTCAGTCTGATTCTGC+TGG 0.484126 1.3:+3972730 MS.gene67121:CDS
ATGTGTTCATCTGAGGTTCC+AGG 0.485714 1.3:-3974266 None:intergenic
GGCATTTGCGTTAGCTGCAA+GGG 0.486589 1.3:+3973974 MS.gene67121:CDS
GAGACCAACCAAGGTTGCTC+TGG 0.487893 1.3:-3974079 None:intergenic
ATACGCTCGTTGGCATCCTC+AGG 0.491563 1.3:-3972600 None:intergenic
CTGTCAATGACGGCAAGTAA+TGG 0.494728 1.3:+3974773 MS.gene67121:CDS
GATTTGAGATGGGCTGGGAC+AGG 0.504393 1.3:-3974442 None:intergenic
TTGCAAAGCTGTGGATCTAA+AGG 0.505094 1.3:-3974650 None:intergenic
ATACTGGTCTTGTCAGGAAA+GGG 0.511385 1.3:+3973918 MS.gene67121:CDS
CTTGAGATTTGAGATGGGCT+GGG 0.511450 1.3:-3974447 None:intergenic
TCAGCTGCCTTGCAAAGCTG+TGG 0.521436 1.3:-3974659 None:intergenic
TGGCATTTGCGTTAGCTGCA+AGG 0.531152 1.3:+3973973 MS.gene67121:CDS
CCAAGCATCCCAAAGATGCT+TGG 0.531277 1.3:+3974035 MS.gene67121:CDS
GATAAGCTGATGGAAACTAG+TGG 0.534659 1.3:+3974749 MS.gene67121:CDS
AGATTAGACTCAACATAGAC+AGG 0.537946 1.3:-3974101 None:intergenic
AAAGGAACCTTACCGAGAAA+GGG 0.538294 1.3:-3974632 None:intergenic
GAGATACACCAGAGCAACCT+TGG 0.541471 1.3:+3974071 MS.gene67121:CDS
GATACTGGTCTTGTCAGGAA+AGG 0.542244 1.3:+3973917 MS.gene67121:CDS
TTGTCAGGAAAGGGAGGTGT+TGG 0.547516 1.3:+3973927 MS.gene67121:CDS
AGGTATATCTCCGTTCTCCA+TGG 0.548279 1.3:-3972580 None:intergenic
TACACCAGAGCAACCTTGGT+TGG 0.551299 1.3:+3974075 MS.gene67121:CDS
ATAACAGCTTCGTCTGGATG+AGG 0.554608 1.3:-3974152 None:intergenic
AATTGAAATGTGTTCATCTG+AGG 0.559191 1.3:-3974273 None:intergenic
ATAAACTTGAGATTTGAGAT+GGG 0.559421 1.3:-3974452 None:intergenic
AAAGATAATAGATAAGCTGA+TGG 0.562227 1.3:+3974739 MS.gene67121:CDS
GGTTGCCATTGCTGAAAGAA+TGG 0.567541 1.3:+3973878 MS.gene67121:CDS
AAATATATTCATACGCTCGT+TGG 0.569537 1.3:-3972610 None:intergenic
AGTGGCCATTCTTTCAGCAA+TGG 0.570251 1.3:-3973883 None:intergenic
ACTATCAAATACTTCACTGC+AGG 0.571986 1.3:-3974567 None:intergenic
CTATGTTGAGTCTAATCTTG+GGG 0.574536 1.3:+3974106 MS.gene67121:CDS
AACTAGTGGGCTGTCAATGA+CGG 0.579345 1.3:+3974763 MS.gene67121:CDS
GTGGCATTTACAGATTGCCC+AGG 0.582123 1.3:+3972709 MS.gene67121:intron
GACGAAGCTGTTATATGGAG+AGG 0.584494 1.3:+3974161 MS.gene67121:CDS
AGAAGAGCTTCCATGGAGAA+CGG 0.601117 1.3:+3972570 None:intergenic
ATAAGCTGATGGAAACTAGT+GGG 0.602164 1.3:+3974750 MS.gene67121:CDS
TTTAGATCCACAGCTTTGCA+AGG 0.603851 1.3:+3974652 MS.gene67121:CDS
GTTGAAGATGTGTAACGAGA+TGG 0.614173 1.3:+3974604 MS.gene67121:CDS
CAGAATCAGACTGAGGACCT+GGG 0.614924 1.3:-3972726 None:intergenic
AGTCGGAGTGAAAGTTCTTG+GGG 0.628178 1.3:+3974400 MS.gene67121:CDS
AAGAGAGACTTGTTGAGGAG+TGG 0.628280 1.3:-3974342 None:intergenic
AGATGAAAGATGTTACGGAG+TGG 0.628653 1.3:+3974495 MS.gene67121:CDS
CTAGATGCTGCTAATGTAGA+CGG 0.629983 1.3:+3974305 MS.gene67121:CDS
CTGGTCTTGTCAGGAAAGGG+AGG 0.640582 1.3:+3973921 MS.gene67121:CDS
TCAATAAGAGAGACTTGTTG+AGG 0.641146 1.3:-3974347 None:intergenic
GAAGTATTTGATAGTAGCCG+TGG 0.649921 1.3:+3974575 MS.gene67121:CDS
GTATTTGATAGTAGCCGTGG+TGG 0.653467 1.3:+3974578 MS.gene67121:CDS
GGGGTTGTGGAGAATATGAG+TGG 0.671351 1.3:+3974419 MS.gene67121:CDS
GGAAAATCTGATGCATGTGA+AGG 0.682695 1.3:+3972751 MS.gene67121:CDS
ACATCTTCAACGCTCCACCA+CGG 0.690721 1.3:-3974592 None:intergenic
TTTCCAGCAGAATCAGACTG+AGG 0.699719 1.3:-3972733 None:intergenic
CATAGACAGGAGACCAACCA+AGG 0.702138 1.3:-3974088 None:intergenic
GAAGATGTGTAACGAGATGG+CGG 0.710160 1.3:+3974607 MS.gene67121:CDS
GGAGAACGGAGATATACCTG+AGG 0.730578 1.3:+3972584 MS.gene67121:CDS
CAGTATCTTATGTTTCACAG+TGG 0.751734 1.3:-3973901 None:intergenic
TGTTCATCTGAGGTTCCAGG+CGG 0.754863 1.3:-3974263 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACATAATATTTTTTCTTAA+CGG + chr1.3:3973679-3973698 MS.gene67121:intron 10.0%
!!! AGTTTTTAAAAGATGTTTAT+TGG + chr1.3:3974210-3974229 MS.gene67121:CDS 15.0%
!!! GTTTTTAAAAGATGTTTATT+GGG + chr1.3:3974211-3974230 MS.gene67121:CDS 15.0%
!!! TTATTTTAATAAACTCTACA+GGG - chr1.3:3973033-3973052 None:intergenic 15.0%
!!! TTTTTAAAAGATGTTTATTG+GGG + chr1.3:3974212-3974231 MS.gene67121:CDS 15.0%
!! ATATTTATAATCTGTTTCAG+AGG - chr1.3:3973611-3973630 None:intergenic 20.0%
!! CTGTAGAGTTTATTAAAATA+AGG + chr1.3:3973032-3973051 MS.gene67121:intron 20.0%
!! TGAACAAGCTTATATAAATA+AGG - chr1.3:3973171-3973190 None:intergenic 20.0%
!! TTTATGAAGATTACAGATAA+TGG + chr1.3:3974470-3974489 MS.gene67121:CDS 20.0%
!!! AACATAAAAGCATAAGATAA+AGG - chr1.3:3972838-3972857 None:intergenic 20.0%
!!! ATGCTGATTAAATATTAAGT+TGG - chr1.3:3973582-3973601 None:intergenic 20.0%
!!! ATTAATCTTTTCATTCAAAC+AGG - chr1.3:3972896-3972915 None:intergenic 20.0%
!!! CGGATAATTTCTTAATTTTA+TGG + chr1.3:3973766-3973785 MS.gene67121:intron 20.0%
!!! CTTATTTTAATAAACTCTAC+AGG - chr1.3:3973034-3973053 None:intergenic 20.0%
!!! TATTTTTTCTTAACGGAAAT+TGG + chr1.3:3973686-3973705 MS.gene67121:intron 20.0%
!!! TTAATCTTTTCATTCAAACA+GGG - chr1.3:3972895-3972914 None:intergenic 20.0%
! AAAGATAATAGATAAGCTGA+TGG + chr1.3:3974739-3974758 MS.gene67121:CDS 25.0%
! AAATATGACATCAAGAAAGT+AGG - chr1.3:3973720-3973739 None:intergenic 25.0%
! ACCATCAACTACTTATATTT+AGG + chr1.3:3972869-3972888 MS.gene67121:intron 25.0%
! ATCATTCTCATTGCTTATTT+GGG + chr1.3:3973293-3973312 MS.gene67121:intron 25.0%
! ATTTGTTCAATTTGTGAAGT+TGG + chr1.3:3973507-3973526 MS.gene67121:intron 25.0%
! CAGATTATAAATATTCTCTC+TGG + chr1.3:3973617-3973636 MS.gene67121:intron 25.0%
! TATAGGGTTATTAATTCCTT+GGG + chr1.3:3973452-3973471 MS.gene67121:intron 25.0%
! TGAAACAATGGCAAAATAAA+AGG - chr1.3:3973645-3973664 None:intergenic 25.0%
! TTATAGGGTTATTAATTCCT+TGG + chr1.3:3973451-3973470 MS.gene67121:intron 25.0%
!! AAAATGATACTTTGAGTTCT+AGG + chr1.3:3973191-3973210 MS.gene67121:intron 25.0%
!! ATAAAAGCATAAGATAAAGG+CGG - chr1.3:3972835-3972854 None:intergenic 25.0%
!! ATAAACTTGAGATTTGAGAT+GGG - chr1.3:3974455-3974474 None:intergenic 25.0%
!! TCTATTATCTTTTGTAATGC+TGG - chr1.3:3974731-3974750 None:intergenic 25.0%
!!! TATAAGTAGTTGATGGTTAT+TGG - chr1.3:3972866-3972885 None:intergenic 25.0%
!!! TATTGTTTGGTCTTGTTATA+GGG + chr1.3:3973436-3973455 MS.gene67121:intron 25.0%
!!! TGCTTAGTTTGTTTATTGTT+TGG + chr1.3:3973423-3973442 MS.gene67121:intron 25.0%
!!! TTATTGTTTGGTCTTGTTAT+AGG + chr1.3:3973435-3973454 MS.gene67121:intron 25.0%
!!! TTCTTGTGTTTTTTGTATTG+TGG + chr1.3:3972690-3972709 MS.gene67121:intron 25.0%
!!! TTTGAGCATTTTTTTAACCT+TGG + chr1.3:3973227-3973246 MS.gene67121:intron 25.0%
AAATATATTCATACGCTCGT+TGG - chr1.3:3972613-3972632 None:intergenic 30.0%
AATTGAAATGTGTTCATCTG+AGG - chr1.3:3974276-3974295 None:intergenic 30.0%
ACCAAAATGCTTAAACATGA+AGG - chr1.3:3973818-3973837 None:intergenic 30.0%
CAATTAATCGTTTACCATGT+CGG + chr1.3:3972922-3972941 MS.gene67121:intron 30.0%
CATAAACTTGAGATTTGAGA+TGG - chr1.3:3974456-3974475 None:intergenic 30.0%
CATCATTCTCATTGCTTATT+TGG + chr1.3:3973292-3973311 MS.gene67121:intron 30.0%
GAATAAAATCAGCTCTTTCA+TGG + chr1.3:3973334-3973353 MS.gene67121:intron 30.0%
GATAAGCTTAAATAAACCAG+TGG + chr1.3:3973137-3973156 MS.gene67121:intron 30.0%
GCCTAAATATAAGTAGTTGA+TGG - chr1.3:3972873-3972892 None:intergenic 30.0%
GCGATTAAATTCAACATTTC+AGG - chr1.3:3974548-3974567 None:intergenic 30.0%
GTTCATTTCATAGTTGTCAA+AGG - chr1.3:3973537-3973556 None:intergenic 30.0%
TACAGGGTAACTTACAAAAA+CGG - chr1.3:3973017-3973036 None:intergenic 30.0%
TATCTAATACATAAGCACCA+AGG - chr1.3:3973247-3973266 None:intergenic 30.0%
TCTATGTTGAGTCTAATCTT+GGG + chr1.3:3974105-3974124 MS.gene67121:CDS 30.0%
TGCCATTGTTTCAAAGTTAA+TGG + chr1.3:3973652-3973671 MS.gene67121:intron 30.0%
! GTGAATTTTTGCAAGAAAGT+CGG + chr1.3:3974383-3974402 MS.gene67121:CDS 30.0%
! TTTTCATTGTTCTTTTGCCT+TGG + chr1.3:3973842-3973861 MS.gene67121:intron 30.0%
!! ATACTTTGAGTTCTAGGATT+AGG + chr1.3:3973197-3973216 MS.gene67121:intron 30.0%
!! CACCATTAACTTTGAAACAA+TGG - chr1.3:3973657-3973676 None:intergenic 30.0%
!! TACTTTGAGTTCTAGGATTA+GGG + chr1.3:3973198-3973217 MS.gene67121:intron 30.0%
!!! CGTATGACATTTTTAAAGTC+AGG + chr1.3:3973370-3973389 MS.gene67121:intron 30.0%
AACACAAGAATCAAAGTCAG+TGG - chr1.3:3972680-3972699 None:intergenic 35.0%
AACCATATCTGCAAAAACCA+AGG - chr1.3:3973862-3973881 None:intergenic 35.0%
AACTTACGACATATCTACAC+AGG - chr1.3:3973073-3973092 None:intergenic 35.0%
ACTATCAAATACTTCACTGC+AGG - chr1.3:3974570-3974589 None:intergenic 35.0%
AGATTAGACTCAACATAGAC+AGG - chr1.3:3974104-3974123 None:intergenic 35.0%
ATAAGCTGATGGAAACTAGT+GGG + chr1.3:3974750-3974769 MS.gene67121:CDS 35.0%
ATTGAATGATGTCCAACACA+TGG - chr1.3:3973559-3973578 None:intergenic 35.0%
CACTGTGAAACATAAGATAC+TGG + chr1.3:3973902-3973921 MS.gene67121:CDS 35.0%
CAGTATCTTATGTTTCACAG+TGG - chr1.3:3973904-3973923 None:intergenic 35.0%
CTATGAAATGAACCATGTGT+TGG + chr1.3:3973544-3973563 MS.gene67121:intron 35.0%
CTATGTTGAGTCTAATCTTG+GGG + chr1.3:3974106-3974125 MS.gene67121:CDS 35.0%
GTCTATGTTGAGTCTAATCT+TGG + chr1.3:3974104-3974123 MS.gene67121:CDS 35.0%
TGGTGAGATGAAAGATGTTA+CGG + chr1.3:3974490-3974509 MS.gene67121:CDS 35.0%
TTGTAGTGTGATTTCTTTCC+AGG + chr1.3:3973267-3973286 MS.gene67121:intron 35.0%
! AAATAAGGACTTGTTTCCAC+TGG - chr1.3:3973156-3973175 None:intergenic 35.0%
! GCCTTCATGTTTAAGCATTT+TGG + chr1.3:3973814-3973833 MS.gene67121:intron 35.0%
! TAGATCGTGTTTTGCAGATA+AGG + chr1.3:3974688-3974707 MS.gene67121:CDS 35.0%
!! CTTATATATAAGCACCGACA+TGG - chr1.3:3972939-3972958 None:intergenic 35.0%
!! TACGACAATTGAGCTGTTTT+CGG + chr1.3:3973746-3973765 MS.gene67121:intron 35.0%
!! TCAATAAGAGAGACTTGTTG+AGG - chr1.3:3974350-3974369 None:intergenic 35.0%
!! TGGCACAAATTTGCTGATTT+GGG - chr1.3:3972781-3972800 None:intergenic 35.0%
!! TGGGTTGATTAGCAAAGAAA+TGG + chr1.3:3973312-3973331 MS.gene67121:intron 35.0%
!!! GATTTTCAAGTTGGTCTTCT+GGG + chr1.3:3973996-3974015 MS.gene67121:CDS 35.0%
!!! GTGGGTATAGTTTTGTGTAA+AGG + chr1.3:3973484-3973503 MS.gene67121:intron 35.0%
AAAGGAACCTTACCGAGAAA+GGG - chr1.3:3974635-3974654 None:intergenic 40.0%
AAAGTCGGAGTGAAAGTTCT+TGG + chr1.3:3974398-3974417 MS.gene67121:CDS 40.0%
AAGTCGGAGTGAAAGTTCTT+GGG + chr1.3:3974399-3974418 MS.gene67121:CDS 40.0%
AATCTTGGGGTCATGTCAAT+TGG + chr1.3:3974119-3974138 MS.gene67121:CDS 40.0%
AGATGAAAGATGTTACGGAG+TGG + chr1.3:3974495-3974514 MS.gene67121:CDS 40.0%
ATCAGCTCTTTCATGGTTCA+TGG + chr1.3:3973341-3973360 MS.gene67121:intron 40.0%
ATCCAGACGAAGCTGTTATA+TGG + chr1.3:3974156-3974175 MS.gene67121:CDS 40.0%
ATCTTGGGGTCATGTCAATT+GGG + chr1.3:3974120-3974139 MS.gene67121:CDS 40.0%
CTAGATGCTGCTAATGTAGA+CGG + chr1.3:3974305-3974324 MS.gene67121:CDS 40.0%
GATAAGCTGATGGAAACTAG+TGG + chr1.3:3974749-3974768 MS.gene67121:CDS 40.0%
GGAAAATCTGATGCATGTGA+AGG + chr1.3:3972751-3972770 MS.gene67121:CDS 40.0%
GTTGAAGATGTGTAACGAGA+TGG + chr1.3:3974604-3974623 MS.gene67121:CDS 40.0%
TAAAGGAACCTTACCGAGAA+AGG - chr1.3:3974636-3974655 None:intergenic 40.0%
TTGCAAAGCTGTGGATCTAA+AGG - chr1.3:3974653-3974672 None:intergenic 40.0%
TTTAGATCCACAGCTTTGCA+AGG + chr1.3:3974652-3974671 MS.gene67121:CDS 40.0%
! GAAGTATTTGATAGTAGCCG+TGG + chr1.3:3974575-3974594 MS.gene67121:CDS 40.0%
! GTGACTTGTGAAACTGCTTA+TGG - chr1.3:3973100-3973119 None:intergenic 40.0%
!! ATACTGGTCTTGTCAGGAAA+GGG + chr1.3:3973918-3973937 MS.gene67121:CDS 40.0%
!! CATAAGATACTGGTCTTGTC+AGG + chr1.3:3973912-3973931 MS.gene67121:CDS 40.0%
!! CTTCTGGGCATTGATATTTG+TGG + chr1.3:3974011-3974030 MS.gene67121:CDS 40.0%
!! GTGGCACAAATTTGCTGATT+TGG - chr1.3:3972782-3972801 None:intergenic 40.0%
!! TGCCTTGGTTTTTGCAGATA+TGG + chr1.3:3973857-3973876 MS.gene67121:intron 40.0%
!! TTGATAAGCCCATTTTTGCG+TGG - chr1.3:3974188-3974207 None:intergenic 40.0%
!!! GGATTTTCAAGTTGGTCTTC+TGG + chr1.3:3973995-3974014 MS.gene67121:CDS 40.0%
AGCAATGAGAATGATGAGCC+TGG - chr1.3:3973288-3973307 None:intergenic 45.0%
AGTCGGAGTGAAAGTTCTTG+GGG + chr1.3:3974400-3974419 MS.gene67121:CDS 45.0%
AGTGAAAGTTCTTGGGGTTG+TGG + chr1.3:3974406-3974425 MS.gene67121:CDS 45.0%
AGTGGCCATTCTTTCAGCAA+TGG - chr1.3:3973886-3973905 None:intergenic 45.0%
ATAACAGCTTCGTCTGGATG+AGG - chr1.3:3974155-3974174 None:intergenic 45.0%
ATTCCTTGGGCATTTGACAG+TGG + chr1.3:3973465-3973484 MS.gene67121:intron 45.0%
CGTCTACATTAGCAGCATCT+AGG - chr1.3:3974307-3974326 None:intergenic 45.0%
CTCCATATAACAGCTTCGTC+TGG - chr1.3:3974161-3974180 None:intergenic 45.0%
CTGTCAATGACGGCAAGTAA+TGG + chr1.3:3974773-3974792 MS.gene67121:CDS 45.0%
CTTCTAGGCCAAGCATCTTT+GGG - chr1.3:3974046-3974065 None:intergenic 45.0%
GAAGATGTGTAACGAGATGG+CGG + chr1.3:3974607-3974626 MS.gene67121:CDS 45.0%
GACGAAGCTGTTATATGGAG+AGG + chr1.3:3974161-3974180 MS.gene67121:CDS 45.0%
GGTGTATCTCTTGACCTTCT+AGG - chr1.3:3974061-3974080 None:intergenic 45.0%
GGTTGCCATTGCTGAAAGAA+TGG + chr1.3:3973878-3973897 MS.gene67121:CDS 45.0%
TTCCTTGGGCATTTGACAGT+GGG + chr1.3:3973466-3973485 MS.gene67121:intron 45.0%
TTTCCAGCAGAATCAGACTG+AGG - chr1.3:3972736-3972755 None:intergenic 45.0%
! ACTTGAGATTTGAGATGGGC+TGG - chr1.3:3974451-3974470 None:intergenic 45.0%
! ATGTGTTCATCTGAGGTTCC+AGG - chr1.3:3974269-3974288 None:intergenic 45.0%
! CTTGAGATTTGAGATGGGCT+GGG - chr1.3:3974450-3974469 None:intergenic 45.0%
! GTATTTGATAGTAGCCGTGG+TGG + chr1.3:3974578-3974597 MS.gene67121:CDS 45.0%
!! AACTAGTGGGCTGTCAATGA+CGG + chr1.3:3974763-3974782 MS.gene67121:CDS 45.0%
!! AAGAGAGACTTGTTGAGGAG+TGG - chr1.3:3974345-3974364 None:intergenic 45.0%
!! GATACTGGTCTTGTCAGGAA+AGG + chr1.3:3973917-3973936 MS.gene67121:CDS 45.0%
!!! GCTGCAAGGGATTTTCAAGT+TGG + chr1.3:3973987-3974006 MS.gene67121:CDS 45.0%
AACCAGGGTCAGGTCCTTTA+GGG - chr1.3:3972808-3972827 None:intergenic 50.0%
ACATCTTCAACGCTCCACCA+CGG - chr1.3:3974595-3974614 None:intergenic 50.0%
AGATAAAGGCGGAACCAACC+AGG - chr1.3:3972824-3972843 None:intergenic 50.0%
AGCTAACGCAAATGCCAGCT+GGG - chr1.3:3973970-3973989 None:intergenic 50.0%
CAGAATCAGACTGAGGACCT+GGG - chr1.3:3972729-3972748 None:intergenic 50.0%
CATAGACAGGAGACCAACCA+AGG - chr1.3:3974091-3974110 None:intergenic 50.0%
CCAAAGATGCTTGGCCTAGA+AGG + chr1.3:3974044-3974063 MS.gene67121:CDS 50.0%
CCAAGCATCCCAAAGATGCT+TGG + chr1.3:3974035-3974054 MS.gene67121:CDS 50.0%
CCTTCTAGGCCAAGCATCTT+TGG - chr1.3:3974047-3974066 None:intergenic 50.0%
CTAATTGTTGATGCTCCGCC+TGG + chr1.3:3974248-3974267 MS.gene67121:CDS 50.0%
CTTTGCAAGGCAGCTGAAGA+AGG + chr1.3:3974665-3974684 MS.gene67121:CDS 50.0%
GAGATACACCAGAGCAACCT+TGG + chr1.3:3974071-3974090 MS.gene67121:CDS 50.0%
GATAAAGGCGGAACCAACCA+GGG - chr1.3:3972823-3972842 None:intergenic 50.0%
GGAGAACGGAGATATACCTG+AGG + chr1.3:3972584-3972603 MS.gene67121:CDS 50.0%
GGAGAGGTCCACGCAAAAAT+GGG + chr1.3:3974177-3974196 MS.gene67121:CDS 50.0%
GGCATTTGCGTTAGCTGCAA+GGG + chr1.3:3973974-3973993 MS.gene67121:CDS 50.0%
GGGGTTGTGGAGAATATGAG+TGG + chr1.3:3974419-3974438 MS.gene67121:CDS 50.0%
GTGGCATTTACAGATTGCCC+AGG + chr1.3:3972709-3972728 MS.gene67121:intron 50.0%
TACACCAGAGCAACCTTGGT+TGG + chr1.3:3974075-3974094 MS.gene67121:CDS 50.0%
TACCCACTGTCAAATGCCCA+AGG - chr1.3:3973471-3973490 None:intergenic 50.0%
TGGAGAGGTCCACGCAAAAA+TGG + chr1.3:3974176-3974195 MS.gene67121:CDS 50.0%
TGGCATTTGCGTTAGCTGCA+AGG + chr1.3:3973973-3973992 MS.gene67121:CDS 50.0%
! ATTTGTGCCACTGCCCCTAA+AGG + chr1.3:3972791-3972810 MS.gene67121:intron 50.0%
! TGTTCATCTGAGGTTCCAGG+CGG - chr1.3:3974266-3974285 None:intergenic 50.0%
! TTGTCAGGAAAGGGAGGTGT+TGG + chr1.3:3973927-3973946 MS.gene67121:CDS 50.0%
!! CCAAGCATCTTTGGGATGCT+TGG - chr1.3:3974038-3974057 None:intergenic 50.0%
ACCAGGGTCAGGTCCTTTAG+GGG - chr1.3:3972807-3972826 None:intergenic 55.0%
ATACGCTCGTTGGCATCCTC+AGG - chr1.3:3972603-3972622 None:intergenic 55.0%
CAACCAGGGTCAGGTCCTTT+AGG - chr1.3:3972809-3972828 None:intergenic 55.0%
CAGCTAACGCAAATGCCAGC+TGG - chr1.3:3973971-3973990 None:intergenic 55.0%
CTAAAGGACCTGACCCTGGT+TGG + chr1.3:3972807-3972826 MS.gene67121:intron 55.0%
GAGACCAACCAAGGTTGCTC+TGG - chr1.3:3974082-3974101 None:intergenic 55.0%
GCAGAATCAGACTGAGGACC+TGG - chr1.3:3972730-3972749 None:intergenic 55.0%
GGTCCTCAGTCTGATTCTGC+TGG + chr1.3:3972730-3972749 MS.gene67121:CDS 55.0%
TCAGCTGCCTTGCAAAGCTG+TGG - chr1.3:3974662-3974681 None:intergenic 55.0%
! GAGATGGCGGTACCCTTTCT+CGG + chr1.3:3974620-3974639 MS.gene67121:CDS 55.0%
! GAGCACATTTTCTGCCCAGC+TGG + chr1.3:3973953-3973972 MS.gene67121:CDS 55.0%
!! CTGGTCTTGTCAGGAAAGGG+AGG + chr1.3:3973921-3973940 MS.gene67121:CDS 55.0%
!! GATTTGAGATGGGCTGGGAC+AGG - chr1.3:3974445-3974464 None:intergenic 55.0%
GGCGGTACCCTTTCTCGGTA+AGG + chr1.3:3974625-3974644 MS.gene67121:CDS 60.0%
! GTCAGGTCCTTTAGGGGCAG+TGG - chr1.3:3972801-3972820 None:intergenic 60.0%
AGGCGGAACCAACCAGGGTC+AGG - chr1.3:3972818-3972837 None:intergenic 65.0%
GCCCCTAAAGGACCTGACCC+TGG + chr1.3:3972803-3972822 MS.gene67121:intron 65.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 3972582 3974802 3972582 ID=MS.gene67121
chr1.3 mRNA 3972582 3974802 3972582 ID=MS.gene67121.t1;Parent=MS.gene67121
chr1.3 exon 3972582 3972618 3972582 ID=MS.gene67121.t1.exon1;Parent=MS.gene67121.t1
chr1.3 CDS 3972582 3972618 3972582 ID=cds.MS.gene67121.t1;Parent=MS.gene67121.t1
chr1.3 exon 3972722 3972772 3972722 ID=MS.gene67121.t1.exon2;Parent=MS.gene67121.t1
chr1.3 CDS 3972722 3972772 3972722 ID=cds.MS.gene67121.t1;Parent=MS.gene67121.t1
chr1.3 exon 3973874 3974802 3973874 ID=MS.gene67121.t1.exon3;Parent=MS.gene67121.t1
chr1.3 CDS 3973874 3974802 3973874 ID=cds.MS.gene67121.t1;Parent=MS.gene67121.t1
Gene Sequence

>MS.gene67121

ATGGAGAACGGAGATATACCTGAGGATGCCAACGAGCGTATGAATATATTTATTAATTATTATTATGTGTTTGAATTTTGCTGTTTCTATATATTCCACTGACTTTGATTCTTGTGTTTTTTGTATTGTGGCATTTACAGATTGCCCAGGTCCTCAGTCTGATTCTGCTGGAAAATCTGATGCATGTGAAGGTTTGCCCAAATCAGCAAATTTGTGCCACTGCCCCTAAAGGACCTGACCCTGGTTGGTTCCGCCTTTATCTTATGCTTTTATGTTTATTTCCAATAACCATCAACTACTTATATTTAGGCCCTGTTTGAATGAAAAGATTAATTAAACGCAATTAATCGTTTACCATGTCGGTGCTTATATATAAGCTATTTTGATAACAGATGATAAAATAAATCAAAGTGTTTTCATATAAACTATGAGCCGTTTTTGTAAGTTACCCTGTAGAGTTTATTAAAATAAGGTGAAAACAGTGTGTGCCTGTGTAGATATGTCGTAAGTTGTGTCCATAAGCAGTTTCACAAGTCACACATGCTTATGTTAGTAGATAAGCTTAAATAAACCAGTGGAAACAAGTCCTTATTTATATAAGCTTGTTCAAAAATGATACTTTGAGTTCTAGGATTAGGGACTGTTTTTGAGCATTTTTTTAACCTTGGTGCTTATGTATTAGATATTGTAGTGTGATTTCTTTCCAGGCTCATCATTCTCATTGCTTATTTGGGTTGATTAGCAAAGAAATGGAATAAAATCAGCTCTTTCATGGTTCATGGCGTTGTCGTATGACATTTTTAAAGTCAGGACTAGTGAAACATTTTATGTGATATTGTTTTGCTTAGTTTGTTTATTGTTTGGTCTTGTTATAGGGTTATTAATTCCTTGGGCATTTGACAGTGGGTATAGTTTTGTGTAAAGGATTTGTTCAATTTGTGAAGTTGGAGATCCTTTGACAACTATGAAATGAACCATGTGTTGGACATCATTCAATCCAACTTAATATTTAATCAGCATGTTTTTCCTCTGAAACAGATTATAAATATTCTCTCTGGATCCTTTTATTTTGCCATTGTTTCAAAGTTAATGGTGATAACATAATATTTTTTCTTAACGGAAATTGGTAGAAATACCTACTTTCTTGATGTCATATTTTTTCTGTACGACAATTGAGCTGTTTTCGGATAATTTCTTAATTTTATGGAATTGAGAATGTTGAGCAACAAATTGCCTTCATGTTTAAGCATTTTGGTGACATTTTCATTGTTCTTTTGCCTTGGTTTTTGCAGATATGGTTGCCATTGCTGAAAGAATGGCCACTGTGAAACATAAGATACTGGTCTTGTCAGGAAAGGGAGGTGTTGGCAAGAGCACATTTTCTGCCCAGCTGGCATTTGCGTTAGCTGCAAGGGATTTTCAAGTTGGTCTTCTGGGCATTGATATTTGTGGTCCAAGCATCCCAAAGATGCTTGGCCTAGAAGGTCAAGAGATACACCAGAGCAACCTTGGTTGGTCTCCTGTCTATGTTGAGTCTAATCTTGGGGTCATGTCAATTGGGTTCATGCTTCCTCATCCAGACGAAGCTGTTATATGGAGAGGTCCACGCAAAAATGGGCTTATCAAGCAGTTTTTAAAAGATGTTTATTGGGGTGAACTTGATTTTCTAATTGTTGATGCTCCGCCTGGAACCTCAGATGAACACATTTCAATTGTTCAATGCCTAGATGCTGCTAATGTAGACGGTGCAATCATAGTTACCACTCCTCAACAAGTCTCTCTTATTGATGAGAAAAAAGAAGTGAATTTTTGCAAGAAAGTCGGAGTGAAAGTTCTTGGGGTTGTGGAGAATATGAGTGGCCTGTCCCAGCCCATCTCAAATCTCAAGTTTATGAAGATTACAGATAATGGTGAGATGAAAGATGTTACGGAGTGGATATCAGAATACATGAAAGAAAAAGCGCCTGAAATGTTGAATTTAATCGCCTGCAGTGAAGTATTTGATAGTAGCCGTGGTGGAGCGTTGAAGATGTGTAACGAGATGGCGGTACCCTTTCTCGGTAAGGTTCCTTTAGATCCACAGCTTTGCAAGGCAGCTGAAGAAGGTAGATCGTGTTTTGCAGATAAGGATTGTGTCGTGAGCGTTCCAGCATTACAAAAGATAATAGATAAGCTGATGGAAACTAGTGGGCTGTCAATGACGGCAAGTAATGGAGTGTAG
Protein sequence

>MS.gene67121.t1

MENGDIPEDANEHCPGPQSDSAGKSDACEDMVAIAERMATVKHKILVLSGKGGVGKSTFSAQLAFALAARDFQVGLLGIDICGPSIPKMLGLEGQEIHQSNLGWSPVYVESNLGVMSIGFMLPHPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLIVDAPPGTSDEHISIVQCLDAANVDGAIIVTTPQQVSLIDEKKEVNFCKKVGVKVLGVVENMSGLSQPISNLKFMKITDNGEMKDVTEWISEYMKEKAPEMLNLIACSEVFDSSRGGALKMCNEMAVPFLGKVPLDPQLCKAAEEGRSCFADKDCVVSVPALQKIIDKLMETSGLSMTASNGV