Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67266.t1 | AFK35673.1 | 81.8 | 77 | 2 | 1 | 1 | 65 | 16 | 92 | 1.80E-24 | 122.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67266.t1 | Q43207 | 66.7 | 75 | 12 | 2 | 3 | 65 | 21 | 94 | 2.3e-17 | 89.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67266.t1 | G7LF61 | 81.8 | 77 | 2 | 1 | 1 | 65 | 16 | 92 | 1.3e-24 | 122.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049262 | MS.gene67266 | 0.822584 | 2.19E-53 | -1.69E-46 |
| MS.gene050611 | MS.gene67266 | 0.821733 | 3.45E-53 | -1.69E-46 |
| MS.gene058515 | MS.gene67266 | 0.801381 | 9.25E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTATGTGTAGTTCATTATAC+TGG | 0.313269 | 5.1:-56762084 | MS.gene67266:intron |
| ACTACTTTCAATTTCACTCT+TGG | 0.361943 | 5.1:-56762015 | MS.gene67266:CDS |
| AGTTTGATTCTAGCAGAGAT+AGG | 0.388381 | 5.1:-56762041 | MS.gene67266:CDS |
| TCCCACATCGTACTATTATC+AGG | 0.413728 | 5.1:-56761442 | MS.gene67266:CDS |
| TATACTGGAACATTGCTTGA+TGG | 0.417512 | 5.1:-56762069 | MS.gene67266:CDS |
| CTTCAAGAATAATAATAGAT+AGG | 0.425201 | 5.1:+56761818 | None:intergenic |
| AAGAAGAAGCTTCTCAAAGA+AGG | 0.467998 | 5.1:-56762202 | MS.gene67266:CDS |
| GTTTGATTCTAGCAGAGATA+GGG | 0.475863 | 5.1:-56762040 | MS.gene67266:CDS |
| TAGGCAGATGGAAGGTGATA+AGG | 0.476180 | 5.1:+56761837 | None:intergenic |
| ATCTATTATTATTCTTGAAG+AGG | 0.490271 | 5.1:-56761815 | MS.gene67266:intron |
| GACTTCGACTTCGCCGGTGC+CGG | 0.516399 | 5.1:-56762280 | MS.gene67266:CDS |
| AATGAAATTAAAAGCATGGC+AGG | 0.519602 | 5.1:-56761474 | MS.gene67266:CDS |
| AATTGGATTATCGCCGGCAC+CGG | 0.525961 | 5.1:+56762267 | None:intergenic |
| GTGGGTACTAGAGAATTCAT+AGG | 0.534544 | 5.1:-56761416 | MS.gene67266:CDS |
| TTCAATTTCACTCTTGGACA+AGG | 0.538952 | 5.1:-56762009 | MS.gene67266:intron |
| GTCAAATGAAATTAAAAGCA+TGG | 0.545516 | 5.1:-56761478 | MS.gene67266:CDS |
| AATAATAATAGATAGGCAGA+TGG | 0.568838 | 5.1:+56761825 | None:intergenic |
| CTTGAGAATTGGATTATCGC+CGG | 0.576084 | 5.1:+56762261 | None:intergenic |
| CATATGTAGACACTGTCATA+GGG | 0.583382 | 5.1:+56761385 | None:intergenic |
| TTTGATTCTAGCAGAGATAG+GGG | 0.593989 | 5.1:-56762039 | MS.gene67266:CDS |
| TCGTACTATTATCAGGCAAG+TGG | 0.597519 | 5.1:-56761435 | MS.gene67266:CDS |
| AAAGACGACTTCGACTTCGC+CGG | 0.605504 | 5.1:-56762286 | None:intergenic |
| AAGCTTCTCAAAGAAGGTGA+AGG | 0.613170 | 5.1:-56762196 | MS.gene67266:intron |
| CGGCGATAATCCAATTCTCA+AGG | 0.616014 | 5.1:-56762260 | MS.gene67266:CDS |
| TGCCTGATAATAGTACGATG+TGG | 0.623695 | 5.1:+56761440 | None:intergenic |
| ATAATAGATAGGCAGATGGA+AGG | 0.627317 | 5.1:+56761829 | None:intergenic |
| CGTACTATTATCAGGCAAGT+GGG | 0.636740 | 5.1:-56761434 | MS.gene67266:CDS |
| GCCTGATAATAGTACGATGT+GGG | 0.647015 | 5.1:+56761441 | None:intergenic |
| GATAATCCAATTCTCAAGGT+CGG | 0.697498 | 5.1:-56762256 | MS.gene67266:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTGATTTTGTTATTTTAATT+GGG | - | chr5.1:56761720-56761739 | MS.gene67266:intron | 10.0% |
| !!! | TTTGATTTTGTTATTTTAAT+TGG | - | chr5.1:56761719-56761738 | MS.gene67266:intron | 10.0% |
| !! | AAATCAAACATAGAAATTAA+CGG | + | chr5.1:56761747-56761766 | None:intergenic | 15.0% |
| !!! | TTAATTTCTATGTTTGATTT+AGG | - | chr5.1:56761747-56761766 | MS.gene67266:intron | 15.0% |
| !! | ATCTATTATTATTCTTGAAG+AGG | - | chr5.1:56761867-56761886 | MS.gene67266:intron | 20.0% |
| !! | CTTCAAGAATAATAATAGAT+AGG | + | chr5.1:56761867-56761886 | None:intergenic | 20.0% |
| !!! | TCTTTTATTTTCTTCACAAA+TGG | + | chr5.1:56762109-56762128 | None:intergenic | 20.0% |
| ! | AATAATAATAGATAGGCAGA+TGG | + | chr5.1:56761860-56761879 | None:intergenic | 25.0% |
| ! | GATTTCAAACAATAAAAAGC+AGG | - | chr5.1:56761957-56761976 | MS.gene67266:intron | 25.0% |
| ! | GTCAAATGAAATTAAAAGCA+TGG | - | chr5.1:56762204-56762223 | MS.gene67266:CDS | 25.0% |
| ! | TTATGTGTAGTTCATTATAC+TGG | - | chr5.1:56761598-56761617 | MS.gene67266:intron | 25.0% |
| !! | ACCTTTAATTTCCTCATAAT+AGG | + | chr5.1:56762050-56762069 | None:intergenic | 25.0% |
| !! | CCTTTAATTTCCTCATAATA+GGG | + | chr5.1:56762049-56762068 | None:intergenic | 25.0% |
| !! | TGTTTGATTTAGGTTATGTT+TGG | - | chr5.1:56761757-56761776 | MS.gene67266:intron | 25.0% |
| !!! | GAGGTAAATTTTTAAAAGTC+AGG | - | chr5.1:56761886-56761905 | MS.gene67266:intron | 25.0% |
| AATCAACGAAGAAAGAACAT+AGG | + | chr5.1:56761538-56761557 | None:intergenic | 30.0% | |
| ACTACTTTCAATTTCACTCT+TGG | - | chr5.1:56761667-56761686 | MS.gene67266:intron | 30.0% | |
| CCCTATTATGAGGAAATTAA+AGG | - | chr5.1:56762046-56762065 | MS.gene67266:CDS | 30.0% | |
| TAGTTATCAGCAAATGAGAT+AGG | - | chr5.1:56762021-56762040 | MS.gene67266:CDS | 30.0% | |
| ! | AATGAAATTAAAAGCATGGC+AGG | - | chr5.1:56762208-56762227 | MS.gene67266:CDS | 30.0% |
| ! | CATTTGCTGATAACTACAAA+AGG | + | chr5.1:56762017-56762036 | None:intergenic | 30.0% |
| !! | TGAAAAGTGCTTATCATATC+AGG | - | chr5.1:56761811-56761830 | MS.gene67266:intron | 30.0% |
| AAAAAGGAAATCGGAAAGCA+AGG | - | chr5.1:56761453-56761472 | MS.gene67266:CDS | 35.0% | |
| AAGAAGAAGCTTCTCAAAGA+AGG | - | chr5.1:56761480-56761499 | MS.gene67266:CDS | 35.0% | |
| AGTTTGATTCTAGCAGAGAT+AGG | - | chr5.1:56761641-56761660 | MS.gene67266:intron | 35.0% | |
| ATAATAGATAGGCAGATGGA+AGG | + | chr5.1:56761856-56761875 | None:intergenic | 35.0% | |
| GATAATCCAATTCTCAAGGT+CGG | - | chr5.1:56761426-56761445 | MS.gene67266:CDS | 35.0% | |
| GTCGGTGAAAAAAAGGAAAT+CGG | - | chr5.1:56761444-56761463 | MS.gene67266:CDS | 35.0% | |
| GTTTGATTCTAGCAGAGATA+GGG | - | chr5.1:56761642-56761661 | MS.gene67266:intron | 35.0% | |
| TATACTGGAACATTGCTTGA+TGG | - | chr5.1:56761613-56761632 | MS.gene67266:intron | 35.0% | |
| TTCAATTTCACTCTTGGACA+AGG | - | chr5.1:56761673-56761692 | MS.gene67266:intron | 35.0% | |
| TTTGATTCTAGCAGAGATAG+GGG | - | chr5.1:56761643-56761662 | MS.gene67266:intron | 35.0% | |
| !! | GCTGCTTACTTTTGATATTG+TGG | - | chr5.1:56762135-56762154 | MS.gene67266:intron | 35.0% |
| AAGCTTCTCAAAGAAGGTGA+AGG | - | chr5.1:56761486-56761505 | MS.gene67266:CDS | 40.0% | |
| CGTACTATTATCAGGCAAGT+GGG | - | chr5.1:56762248-56762267 | MS.gene67266:CDS | 40.0% | |
| CTTGAGAATTGGATTATCGC+CGG | + | chr5.1:56761424-56761443 | None:intergenic | 40.0% | |
| GAGATAGGATCCCTATTATG+AGG | - | chr5.1:56762036-56762055 | MS.gene67266:CDS | 40.0% | |
| GCCTGATAATAGTACGATGT+GGG | + | chr5.1:56762244-56762263 | None:intergenic | 40.0% | |
| GTGGGTACTAGAGAATTCAT+AGG | - | chr5.1:56762266-56762285 | MS.gene67266:CDS | 40.0% | |
| TCCCACATCGTACTATTATC+AGG | - | chr5.1:56762240-56762259 | MS.gene67266:CDS | 40.0% | |
| TCGTACTATTATCAGGCAAG+TGG | - | chr5.1:56762247-56762266 | MS.gene67266:CDS | 40.0% | |
| TCTCAAAGAAGGTGAAGGTT+GGG | - | chr5.1:56761491-56761510 | MS.gene67266:CDS | 40.0% | |
| TCTCAAGGTCGGTGAAAAAA+AGG | - | chr5.1:56761437-56761456 | MS.gene67266:CDS | 40.0% | |
| TGCCTGATAATAGTACGATG+TGG | + | chr5.1:56762245-56762264 | None:intergenic | 40.0% | |
| TTCTCAAAGAAGGTGAAGGT+TGG | - | chr5.1:56761490-56761509 | MS.gene67266:CDS | 40.0% | |
| ! | TTTTCACCGACCTTGAGAAT+TGG | + | chr5.1:56761435-56761454 | None:intergenic | 40.0% |
| AGGTTAGTGCATTGGAACGA+CGG | - | chr5.1:56761693-56761712 | MS.gene67266:intron | 45.0% | |
| CGGCGATAATCCAATTCTCA+AGG | - | chr5.1:56761422-56761441 | MS.gene67266:CDS | 45.0% | |
| CTTGGACAAGGTTAGTGCAT+TGG | - | chr5.1:56761685-56761704 | MS.gene67266:intron | 45.0% | |
| TAGGCAGATGGAAGGTGATA+AGG | + | chr5.1:56761848-56761867 | None:intergenic | 45.0% | |
| AATTGGATTATCGCCGGCAC+CGG | + | chr5.1:56761418-56761437 | None:intergenic | 50.0% | |
| GGTTGGGATGCTCATGATGT+TGG | - | chr5.1:56761507-56761526 | MS.gene67266:intron | 50.0% | |
| GACTTCGACTTCGCCGGTGC+CGG | - | chr5.1:56761402-56761421 | MS.gene67266:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 56761400 | 56762304 | 56761400 | ID=MS.gene67266 |
| chr5.1 | mRNA | 56761400 | 56762304 | 56761400 | ID=MS.gene67266.t1;Parent=MS.gene67266 |
| chr5.1 | exon | 56762197 | 56762304 | 56762197 | ID=MS.gene67266.t1.exon1;Parent=MS.gene67266.t1 |
| chr5.1 | CDS | 56762197 | 56762304 | 56762197 | ID=cds.MS.gene67266.t1;Parent=MS.gene67266.t1 |
| chr5.1 | exon | 56762010 | 56762096 | 56762010 | ID=MS.gene67266.t1.exon2;Parent=MS.gene67266.t1 |
| chr5.1 | CDS | 56762010 | 56762096 | 56762010 | ID=cds.MS.gene67266.t1;Parent=MS.gene67266.t1 |
| chr5.1 | exon | 56761816 | 56761871 | 56761816 | ID=MS.gene67266.t1.exon3;Parent=MS.gene67266.t1 |
| chr5.1 | CDS | 56761816 | 56761871 | 56761816 | ID=cds.MS.gene67266.t1;Parent=MS.gene67266.t1 |
| chr5.1 | exon | 56761400 | 56761513 | 56761400 | ID=MS.gene67266.t1.exon4;Parent=MS.gene67266.t1 |
| chr5.1 | CDS | 56761400 | 56761513 | 56761400 | ID=cds.MS.gene67266.t1;Parent=MS.gene67266.t1 |
| Gene Sequence |
| Protein sequence |
