AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene67307

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67307.t1 MTR_2g435930 98.028 507 10 0 1 507 1 507 0.0 1009
MS.gene67307.t1 MTR_6g043810 45.984 498 253 3 11 507 15 497 3.42e-159 462
MS.gene67307.t1 MTR_6g043810 45.984 498 253 3 11 507 25 507 4.98e-159 462
MS.gene67307.t1 MTR_3g111020 46.458 480 241 2 2 481 34 497 1.17e-150 442
MS.gene67307.t1 MTR_5g067460 45.975 472 237 3 11 481 47 501 3.63e-147 433
MS.gene67307.t1 MTR_0039s0080 43.164 512 260 4 1 507 17 502 4.62e-145 427
MS.gene67307.t1 MTR_1g101500 44.356 505 241 5 8 497 55 534 1.15e-140 416
MS.gene67307.t1 MTR_5g010830 43.644 472 248 3 5 476 53 506 1.01e-134 400
MS.gene67307.t1 MTR_8g102430 42.495 473 233 6 7 476 8 444 9.21e-131 387
MS.gene67307.t1 MTR_6g088805 42.094 468 252 5 17 484 25 473 2.84e-130 387
MS.gene67307.t1 MTR_4g027920 40.299 469 266 2 13 481 150 604 6.69e-120 365
MS.gene67307.t1 MTR_5g011540 32.914 477 305 3 4 480 28 489 1.80e-81 262
MS.gene67307.t1 MTR_6g081410 28.184 479 330 2 1 479 7 471 1.42e-73 241
MS.gene67307.t1 MTR_6g081810 28.964 473 294 3 5 477 13 443 5.12e-72 236
MS.gene67307.t1 MTR_6g081240 31.004 458 292 6 16 469 7 444 3.31e-70 231
MS.gene67307.t1 MTR_6g081400 31.250 464 300 4 16 477 12 458 4.38e-68 226
MS.gene67307.t1 MTR_6g081780 26.598 485 330 3 1 479 7 471 9.51e-68 226
MS.gene67307.t1 MTR_6g081300 29.876 482 311 5 16 492 7 466 1.39e-67 225
MS.gene67307.t1 MTR_6g066380 28.392 479 327 3 1 477 7 471 2.49e-67 224
MS.gene67307.t1 MTR_6g004220 29.724 471 314 3 8 477 12 466 4.19e-66 221
MS.gene67307.t1 MTR_1g108840 28.814 472 319 3 4 474 25 480 2.79e-65 219
MS.gene67307.t1 MTR_1g108810 29.825 456 300 5 16 469 31 468 3.12e-65 219
MS.gene67307.t1 MTR_8g106670 27.254 477 331 2 17 493 10 470 4.54e-65 218
MS.gene67307.t1 MTR_6g004220 29.806 463 308 3 8 469 19 465 5.08e-64 216
MS.gene67307.t1 MTR_6g081310 30.233 473 282 6 16 473 7 446 1.83e-62 211
MS.gene67307.t1 MTR_6g081390 29.526 464 308 4 16 477 9 455 8.45e-61 207
MS.gene67307.t1 MTR_7g082810 28.602 472 315 4 13 481 14 466 1.36e-60 207
MS.gene67307.t1 MTR_1g109060 28.850 461 308 4 19 477 24 466 7.55e-58 199
MS.gene67307.t1 MTR_7g082800 31.266 403 261 2 78 480 1 387 1.25e-56 194
MS.gene67307.t1 MTR_1g109060 28.918 453 302 4 19 469 24 458 1.68e-55 193
MS.gene67307.t1 MTR_1g108990 27.802 464 317 2 19 481 25 471 2.16e-51 182
MS.gene67307.t1 MTR_2g078930 26.362 459 320 3 18 475 42 483 2.75e-49 177
MS.gene67307.t1 MTR_6g004220 29.058 382 254 3 8 388 19 384 6.46e-48 172
MS.gene67307.t1 MTR_8g069470 27.254 488 312 11 6 477 31 491 1.45e-46 169
MS.gene67307.t1 MTR_2g079020 25.862 464 324 4 18 480 36 480 3.82e-45 166
MS.gene67307.t1 MTR_2g078960 25.974 462 326 3 18 479 32 477 9.27e-45 164
MS.gene67307.t1 MTR_4g048000 24.454 458 324 3 20 474 65 503 3.96e-42 157
MS.gene67307.t1 MTR_2g022980 24.619 459 318 5 25 475 50 488 7.45e-42 156
MS.gene67307.t1 MTR_5g033100 24.464 466 331 5 16 479 53 499 8.21e-42 157
MS.gene67307.t1 MTR_2g088400 24.726 457 322 3 20 473 53 490 1.14e-41 156
MS.gene67307.t1 MTR_2g078970 25.054 459 326 3 18 475 30 471 3.55e-41 154
MS.gene67307.t1 MTR_6g047610 26.350 463 298 7 19 469 31 462 4.68e-40 151
MS.gene67307.t1 MTR_5g032720 26.022 465 320 7 14 474 31 475 7.71e-39 148
MS.gene67307.t1 MTR_1g021740 23.480 477 346 3 2 477 18 476 1.11e-38 147
MS.gene67307.t1 MTR_5g032750 25.217 460 326 5 16 474 33 475 4.34e-38 145
MS.gene67307.t1 MTR_8g063590 26.559 433 301 5 50 481 84 500 1.86e-36 141
MS.gene67307.t1 MTR_6g004220 29.073 313 205 3 8 319 19 315 2.01e-36 137
MS.gene67307.t1 MTR_2g088400 24.533 428 300 4 20 444 53 460 6.96e-36 139
MS.gene67307.t1 MTR_5g090290 25.957 470 321 7 31 492 54 504 9.94e-35 137
MS.gene67307.t1 MTR_1g100180 24.834 451 319 4 20 469 44 475 2.72e-34 135
MS.gene67307.t1 MTR_7g087410 24.043 470 328 4 16 479 28 474 8.72e-34 134
MS.gene67307.t1 MTR_3g099720 22.661 481 355 4 4 484 26 489 9.66e-34 134
MS.gene67307.t1 MTR_7g087430 24.816 407 275 3 78 477 1 383 1.30e-32 129
MS.gene67307.t1 MTR_3g099700 24.319 477 339 6 4 477 31 488 2.68e-32 129
MS.gene67307.t1 MTR_7g087400 22.864 433 308 4 49 477 60 470 6.07e-32 128
MS.gene67307.t1 MTR_6g047640 24.009 454 302 4 19 469 31 444 2.27e-31 126
MS.gene67307.t1 MTR_6g047640 24.670 454 318 3 19 469 31 463 7.06e-31 125
MS.gene67307.t1 MTR_7g087410 23.399 406 286 2 78 479 1 385 2.44e-30 122
MS.gene67307.t1 MTR_6g047590 25.221 452 317 6 19 469 31 462 7.43e-29 119
MS.gene67307.t1 MTR_7g087370 21.895 475 316 5 7 477 18 441 2.00e-26 112
MS.gene67307.t1 MTR_6g027200 22.465 503 353 6 3 490 20 500 1.69e-24 107
MS.gene67307.t1 MTR_5g087930 22.757 457 308 9 34 483 41 459 3.96e-22 99.4
MS.gene67307.t1 MTR_6g027190 22.718 471 329 8 8 469 13 457 2.32e-20 94.4
MS.gene67307.t1 MTR_2g078990 25.714 210 156 0 262 471 87 296 6.23e-18 85.1
MS.gene67307.t1 MTR_2g079030 27.404 208 147 3 274 480 319 523 6.02e-16 80.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67307.t1 AT5G49130 62.035 511 173 5 1 500 1 501 0.0 615
MS.gene67307.t1 AT1G71870 45.709 501 261 4 3 493 7 506 1.12e-153 448
MS.gene67307.t1 AT4G29140 43.699 492 254 3 4 495 40 508 5.67e-144 424
MS.gene67307.t1 AT5G19700 43.205 493 256 6 4 492 20 492 6.03e-137 405
MS.gene67307.t1 AT5G52050 41.756 467 262 2 18 484 33 489 6.56e-134 397
MS.gene67307.t1 AT1G58340 43.949 471 248 3 9 479 47 501 2.74e-132 394
MS.gene67307.t1 AT4G23030 43.061 490 244 5 5 483 14 479 4.74e-120 362
MS.gene67307.t1 AT2G38510 36.917 493 292 5 16 507 2 476 4.43e-117 353
MS.gene67307.t1 AT4G22790 33.598 503 299 6 1 492 1 479 1.15e-78 254
MS.gene67307.t1 AT5G52450 29.630 486 322 4 10 492 13 481 5.39e-66 221
MS.gene67307.t1 AT1G15150 30.131 458 300 3 20 475 31 470 6.11e-65 218
MS.gene67307.t1 AT1G15170 30.482 456 301 2 20 475 34 473 1.59e-64 217
MS.gene67307.t1 AT1G64820 30.285 492 318 4 18 505 27 497 2.04e-64 218
MS.gene67307.t1 AT1G71140 29.590 463 306 4 20 480 26 470 1.35e-61 209
MS.gene67307.t1 AT2G04080 29.202 476 317 4 4 477 11 468 1.81e-61 209
MS.gene67307.t1 AT1G66760 29.437 462 310 3 19 480 27 472 2.78e-61 209
MS.gene67307.t1 AT2G04100 28.602 472 320 3 7 477 16 471 7.44e-61 207
MS.gene67307.t1 AT1G15160 30.065 459 297 5 20 474 31 469 9.23e-61 207
MS.gene67307.t1 AT2G04070 29.670 455 304 2 20 474 27 465 3.04e-60 206
MS.gene67307.t1 AT1G15180 31.878 458 292 4 20 475 35 474 4.38e-60 205
MS.gene67307.t1 AT2G34360 27.767 497 324 6 1 487 1 472 5.60e-60 205
MS.gene67307.t1 AT2G04040 29.512 471 315 3 7 477 15 468 9.27e-60 204
MS.gene67307.t1 AT2G04080 29.274 468 311 4 4 469 11 460 9.34e-60 204
MS.gene67307.t1 AT2G04050 31.317 463 298 4 14 474 21 465 1.13e-59 204
MS.gene67307.t1 AT2G04100 28.664 464 314 3 7 469 16 463 3.49e-59 202
MS.gene67307.t1 AT1G66760 29.490 451 302 3 19 469 27 461 1.83e-57 198
MS.gene67307.t1 AT1G66780 29.560 477 315 4 3 477 19 476 7.08e-57 197
MS.gene67307.t1 AT1G73700 28.419 468 319 2 13 480 17 468 2.54e-56 195
MS.gene67307.t1 AT2G04090 27.027 481 330 5 6 483 15 477 2.07e-55 193
MS.gene67307.t1 AT1G15170 30.245 367 240 2 109 475 4 354 1.53e-52 182
MS.gene67307.t1 AT1G11670 26.154 455 320 4 20 474 50 488 4.81e-51 182
MS.gene67307.t1 AT1G61890 26.813 455 313 5 25 475 48 486 2.61e-49 177
MS.gene67307.t1 AT1G61890 25.696 467 327 5 7 469 30 480 6.48e-48 173
MS.gene67307.t1 AT1G15150 29.670 364 236 3 114 475 9 354 4.00e-47 168
MS.gene67307.t1 AT3G23550 27.093 454 309 3 18 468 29 463 2.34e-44 163
MS.gene67307.t1 AT1G66760 29.348 368 244 3 19 386 27 378 3.56e-43 158
MS.gene67307.t1 AT4G21910 25.934 455 320 5 20 474 55 492 8.26e-43 159
MS.gene67307.t1 AT4G21910 25.934 455 320 5 20 474 55 492 8.26e-43 159
MS.gene67307.t1 AT3G21690 25.708 459 317 4 20 474 52 490 9.08e-43 159
MS.gene67307.t1 AT3G59030 27.051 451 311 6 34 483 65 498 1.38e-42 159
MS.gene67307.t1 AT3G26590 24.946 461 319 7 20 475 45 483 2.64e-42 158
MS.gene67307.t1 AT1G61890 25.688 436 304 5 7 438 30 449 5.65e-42 156
MS.gene67307.t1 AT4G21903 26.279 430 290 6 50 474 83 490 6.46e-42 157
MS.gene67307.t1 AT5G38030 24.684 474 327 8 20 485 45 496 6.16e-41 154
MS.gene67307.t1 AT4G21903 25.901 444 298 7 50 484 83 504 6.76e-41 154
MS.gene67307.t1 AT4G21910 25.219 456 324 5 20 475 57 495 6.91e-41 154
MS.gene67307.t1 AT4G21910 25.219 456 324 5 20 475 57 495 6.91e-41 154
MS.gene67307.t1 AT3G23560 25.424 472 326 4 4 468 19 471 1.28e-39 150
MS.gene67307.t1 AT4G21903 25.888 394 276 4 81 474 4 381 1.66e-39 148
MS.gene67307.t1 AT3G26590 24.874 398 276 5 81 475 4 381 4.07e-39 147
MS.gene67307.t1 AT1G12950 24.678 466 318 8 20 477 67 507 7.47e-39 149
MS.gene67307.t1 AT4G00350 25.272 459 323 4 18 475 86 525 1.10e-38 148
MS.gene67307.t1 AT5G44050 25.417 480 325 8 2 474 25 478 4.64e-38 145
MS.gene67307.t1 AT1G66760 27.954 347 234 3 123 469 3 333 4.88e-36 137
MS.gene67307.t1 AT1G47530 23.605 466 331 5 16 477 31 475 7.01e-36 139
MS.gene67307.t1 AT1G23300 25.255 491 325 8 20 499 44 503 8.52e-35 137
MS.gene67307.t1 AT1G15180 30.496 282 176 4 109 388 4 267 7.59e-34 132
MS.gene67307.t1 AT4G25640 22.889 450 329 1 20 469 34 465 8.93e-33 131
MS.gene67307.t1 AT4G25640 22.889 450 329 1 20 469 34 465 1.42e-32 130
MS.gene67307.t1 AT5G10420 24.406 463 326 6 16 474 34 476 5.55e-32 129
MS.gene67307.t1 AT1G33090 25.210 476 321 7 8 469 14 468 5.98e-32 129
MS.gene67307.t1 AT3G23560 25.459 381 258 4 4 377 19 380 9.70e-32 127
MS.gene67307.t1 AT5G65380 25.051 487 332 5 2 477 14 478 1.06e-31 127
MS.gene67307.t1 AT1G33100 24.779 452 319 4 20 469 33 465 8.61e-31 125
MS.gene67307.t1 AT1G47530 23.750 400 280 5 82 477 5 383 3.06e-30 122
MS.gene67307.t1 AT1G33110 24.262 474 328 5 8 469 14 468 1.08e-29 122
MS.gene67307.t1 AT1G33080 23.843 432 300 4 49 474 65 473 2.70e-29 120
MS.gene67307.t1 AT1G33080 23.888 427 296 4 49 469 65 468 1.05e-28 119
MS.gene67307.t1 AT4G25640 22.090 421 310 1 20 440 34 436 2.59e-27 115
MS.gene67307.t1 AT2G34360 26.522 230 162 1 1 223 1 230 4.40e-27 114
MS.gene67307.t1 AT2G34360 33.523 176 115 1 312 487 257 430 4.64e-20 93.2
MS.gene67307.t1 AT3G59030 26.099 364 251 6 34 396 65 411 1.36e-26 112
MS.gene67307.t1 AT3G03620 22.526 475 335 6 3 469 20 469 2.14e-25 109
MS.gene67307.t1 AT3G03620 22.526 475 335 6 3 469 20 469 2.14e-25 109
MS.gene67307.t1 AT5G17700 20.877 479 346 6 5 475 19 472 2.74e-22 100
MS.gene67307.t1 AT5G17700 20.877 479 346 6 5 475 19 472 2.74e-22 100

Find 125 sgRNAs with CRISPR-Local

Find 186 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGCTCACATTTGATTATT+TGG 0.125287 2.3:+55040378 None:intergenic
ACCGGTTACTCAGTCCTTTC+AGG 0.200835 2.3:-55041643 MS.gene67307:CDS
GTTCAACATTAGGGTTATTA+TGG 0.202311 2.3:-55040802 MS.gene67307:CDS
CAGTTGTCGGGTATGTCTTT+TGG 0.247868 2.3:+55042248 None:intergenic
GGAGTGCAAGGCCAGGTATT+GGG 0.276246 2.3:-55040637 MS.gene67307:CDS
AAGAATGGGGCATGTTAATA+AGG 0.284500 2.3:-55041084 MS.gene67307:CDS
GTTGACAAATTTGCCTTTGA+TGG 0.291782 2.3:+55040854 None:intergenic
TGATGAAGGGATAGCATAAA+TGG 0.299467 2.3:+55041478 None:intergenic
ACTATGCTCATGGCTGCTAT+TGG 0.316954 2.3:+55041763 None:intergenic
TCACTAGCAAGTTCAACATT+AGG 0.321641 2.3:-55040812 MS.gene67307:CDS
TGTAGAACATGTATGATAAA+AGG 0.326149 2.3:+55041203 None:intergenic
CACTAGCAAGTTCAACATTA+GGG 0.353538 2.3:-55040811 MS.gene67307:CDS
GAAGAAAAGAGTATTGAAAT+TGG 0.354879 2.3:+55041225 None:intergenic
AACTCACAAACACCAATTAT+AGG 0.359394 2.3:+55040701 None:intergenic
GGGTTATTATGGACAACATT+AGG 0.362622 2.3:-55040791 MS.gene67307:CDS
TGAGTTAGCAAGTGGTGCTT+TGG 0.364654 2.3:-55041686 MS.gene67307:CDS
AAAGGCAAATTTGTCAACTA+TGG 0.374974 2.3:-55040849 MS.gene67307:CDS
ACTAGTTGTGGAATGCTTAG+AGG 0.390010 2.3:-55040659 MS.gene67307:CDS
AGTGAAGTGCACCACAATAA+TGG 0.390331 2.3:+55041337 None:intergenic
GCAAGTGGTGCTTTGGCCAT+AGG 0.404906 2.3:-55041679 MS.gene67307:CDS
ATTAAGCCACAAGAGTGAAA+TGG 0.405706 2.3:+55041507 None:intergenic
CTATGCTCATGGCTGCTATT+GGG 0.407811 2.3:+55041764 None:intergenic
TTGTCAACTATGGTAGCAAT+TGG 0.413667 2.3:-55040839 MS.gene67307:CDS
GGGAGTGCAAGGCCAGGTAT+TGG 0.419436 2.3:-55040638 MS.gene67307:CDS
AAGGTTAAGAAAATGATGAT+AGG 0.422391 2.3:+55041295 None:intergenic
ACCTGAAAGGACTGAGTAAC+CGG 0.424046 2.3:+55041642 None:intergenic
AACCCCAACACTATTCCTAT+TGG 0.430761 2.3:+55040575 None:intergenic
GTCTTGGAGTTTGCTTAGAA+TGG 0.433878 2.3:-55041045 MS.gene67307:CDS
CAAAACCCTGGGCAAAGAAT+GGG 0.434167 2.3:-55041098 MS.gene67307:CDS
AGTTGTTTGCATGGGAAAGT+TGG 0.438028 2.3:-55041716 MS.gene67307:CDS
GCGCCAATAGGAATAGTGTT+GGG 0.442712 2.3:-55040578 MS.gene67307:CDS
CATGATCTTAGTTGTTTGCA+TGG 0.446886 2.3:-55041725 MS.gene67307:CDS
TCAAAACCCTGGGCAAAGAA+TGG 0.453731 2.3:-55041099 MS.gene67307:CDS
TTAAGCCACAAGAGTGAAAT+GGG 0.455813 2.3:+55041508 None:intergenic
GTTGTTTGCATGGGAAAGTT+GGG 0.457287 2.3:-55041715 MS.gene67307:CDS
ACCGACGGCACTTAGTGCTT+CGG 0.461028 2.3:-55040915 MS.gene67307:CDS
TACACCAAGGTGAAGTTTGA+AGG 0.461500 2.3:+55041276 None:intergenic
TTTATCCCTGCCCCAATACC+TGG 0.465679 2.3:+55040626 None:intergenic
CATTACCCATTTCACTCTTG+TGG 0.466604 2.3:-55041513 MS.gene67307:CDS
TTGTGGTATATAACACTGAT+TGG 0.471072 2.3:-55040475 MS.gene67307:CDS
ATAGCAGCCATGAGCATAGT+AGG 0.475953 2.3:-55041760 MS.gene67307:CDS
AACTCCAAGACAACTTTGAA+TGG 0.476284 2.3:+55041057 None:intergenic
GCAAGGCCAGGTATTGGGGC+AGG 0.476495 2.3:-55040632 MS.gene67307:CDS
GATGATAACAAGGGAGGACT+AGG 0.479205 2.3:+55041145 None:intergenic
ATAGGCTTCACCAATGTAAC+CGG 0.479266 2.3:-55041661 MS.gene67307:CDS
ATTCCACAACTAGTTGTTTG+TGG 0.480040 2.3:+55040668 None:intergenic
AAACTTCACCTTGGTGTACA+AGG 0.480431 2.3:-55041271 MS.gene67307:CDS
ATCGTACCTCAGCAGTTGTC+GGG 0.482070 2.3:+55042236 None:intergenic
CCCTTGTTGTGATGATAACA+AGG 0.485160 2.3:+55041135 None:intergenic
GAATCGAGAGGCGACAATAG+AGG 0.486395 2.3:+55041428 None:intergenic
GAGGTATCCTACTATGCTCA+TGG 0.490636 2.3:+55041753 None:intergenic
ACATGCCCCATTCTTTGCCC+AGG 0.492834 2.3:+55041092 None:intergenic
CATGCCCCATTCTTTGCCCA+GGG 0.506303 2.3:+55041093 None:intergenic
GAGTGCAAGGCCAGGTATTG+GGG 0.506676 2.3:-55040636 MS.gene67307:CDS
GGTTACATTGGTGAAGCCTA+TGG 0.510196 2.3:+55041663 None:intergenic
AACAAGGGAGGACTAGGAAT+AGG 0.516192 2.3:+55041151 None:intergenic
TACGTAGCAAAGGCACAACT+TGG 0.516312 2.3:-55041360 MS.gene67307:CDS
AATAGATGCGAATCGGATGA+AGG 0.516484 2.3:+55041383 None:intergenic
GCTTCGGTTTCAACAAGGGT+AGG 0.520507 2.3:-55040899 MS.gene67307:CDS
GGTGTTCACAAATGATAAAG+AGG 0.524145 2.3:-55040750 MS.gene67307:CDS
ATGATCTTAGTTGTTTGCAT+GGG 0.527005 2.3:-55041724 MS.gene67307:CDS
GAGGCTATTCGCTATAAGAT+CGG 0.528355 2.3:+55041405 None:intergenic
GAGTTTGCTTAGAATGGTGG+TGG 0.529737 2.3:-55041039 MS.gene67307:CDS
GTAGGGAATGCACTTGGAGC+TGG 0.530874 2.3:-55040881 MS.gene67307:CDS
CTACGTAAATAGATGCGAAT+CGG 0.532204 2.3:+55041376 None:intergenic
AGGCTATTCGCTATAAGATC+GGG 0.539074 2.3:+55041406 None:intergenic
CTAGTTGTGGAATGCTTAGA+GGG 0.539611 2.3:-55040658 MS.gene67307:CDS
CGTGTAGCTTTAGCCACGGC+GGG 0.541296 2.3:-55040974 MS.gene67307:CDS
TTCAGGTCTAGCCATGGGAA+TGG 0.547464 2.3:-55041626 MS.gene67307:CDS
CGCATCTATTTACGTAGCAA+AGG 0.547845 2.3:-55041370 MS.gene67307:CDS
GTCCTTTCAGGTCTAGCCAT+GGG 0.551373 2.3:-55041631 MS.gene67307:CDS
AGGTTAAGAAAATGATGATA+GGG 0.552617 2.3:+55041296 None:intergenic
AAGCTACACGAGGGTTGTAA+AGG 0.553073 2.3:+55040987 None:intergenic
TGTGGTATATAACACTGATT+GGG 0.556810 2.3:-55040474 MS.gene67307:CDS
ACCAACAAGTGTCAAAACCC+TGG 0.556869 2.3:-55041110 MS.gene67307:CDS
GCACAACTTGGCCATTATTG+TGG 0.558363 2.3:-55041348 MS.gene67307:CDS
TGTGGTATCAATTCTTGTTG+TGG 0.558880 2.3:-55040492 MS.gene67307:CDS
TGGAGCTGGTCAACCATCAA+AGG 0.562155 2.3:-55040867 MS.gene67307:CDS
GACTGAGTAACCGGTTACAT+TGG 0.562773 2.3:+55041651 None:intergenic
GGGAGGACTAGGAATAGGTA+TGG 0.566306 2.3:+55041156 None:intergenic
GGACGAGCTAAAGAAAATGA+TGG 0.566710 2.3:-55041797 MS.gene67307:CDS
GATAGGGATGTGAATGATAA+CGG 0.567233 2.3:+55041312 None:intergenic
CGCCAATAGGAATAGTGTTG+GGG 0.568084 2.3:-55040577 MS.gene67307:CDS
CTTAGAGGGAGTGCAAGGCC+AGG 0.570090 2.3:-55040644 MS.gene67307:CDS
GAGTTCATGACAATTCTAGC+TGG 0.573693 2.3:-55041013 MS.gene67307:CDS
CTAAAGAAAATGATGGACAT+AGG 0.574782 2.3:-55041790 MS.gene67307:CDS
CAAGGCCAGGTATTGGGGCA+GGG 0.575030 2.3:-55040631 MS.gene67307:CDS
TTGGAGTTTGCTTAGAATGG+TGG 0.575348 2.3:-55041042 MS.gene67307:CDS
CCTCTATGTACTCAAGCCAT+TGG 0.575821 2.3:-55041601 MS.gene67307:CDS
CCCTTGTTATCATCACAACA+AGG 0.576918 2.3:-55041136 MS.gene67307:CDS
CTTCGGTTTCAACAAGGGTA+GGG 0.577980 2.3:-55040898 MS.gene67307:CDS
TGTCCACAAACAACTAGTTG+TGG 0.580606 2.3:-55040671 MS.gene67307:CDS
GGCGCCAATAGGAATAGTGT+TGG 0.580799 2.3:-55040579 MS.gene67307:CDS
TATCGTACCTCAGCAGTTGT+CGG 0.582160 2.3:+55042235 None:intergenic
ACAAGGGTAGGGAATGCACT+TGG 0.586849 2.3:-55040887 MS.gene67307:CDS
TTAACCTTCAAACTTCACCT+TGG 0.590547 2.3:-55041280 MS.gene67307:CDS
TTGGGAAAGAGAGTCATTGA+AGG 0.590950 2.3:-55040456 MS.gene67307:CDS
GGTAGCCTTGAGTTAGCAAG+TGG 0.598563 2.3:-55041694 MS.gene67307:CDS
AGTCCTTTCAGGTCTAGCCA+TGG 0.601305 2.3:-55041632 MS.gene67307:CDS
CCCTCGTGTAGCTTTAGCCA+CGG 0.602755 2.3:-55040978 MS.gene67307:CDS
AAGCACCACTTGCTAACTCA+AGG 0.604905 2.3:+55041689 None:intergenic
GAATGCTTAGAGGGAGTGCA+AGG 0.609636 2.3:-55040649 MS.gene67307:CDS
TTAGTGCTTCGGTTTCAACA+AGG 0.611369 2.3:-55040904 MS.gene67307:CDS
TTCCCATGGCTAGACCTGAA+AGG 0.612296 2.3:+55041629 None:intergenic
ACATAGAGGCTCCATTCCCA+TGG 0.614544 2.3:+55041615 None:intergenic
CCAACAAGTGTCAAAACCCT+GGG 0.616891 2.3:-55041109 MS.gene67307:CDS
AAAACCCTGGGCAAAGAATG+GGG 0.618666 2.3:-55041097 MS.gene67307:CDS
CCAATGGCTTGAGTACATAG+AGG 0.624591 2.3:+55041601 None:intergenic
ACTTATGTACACTTTACCGA+CGG 0.625353 2.3:-55040930 MS.gene67307:CDS
TTGTATTACTATTCCCGCCG+TGG 0.632361 2.3:+55040961 None:intergenic
TAGTGCTTCGGTTTCAACAA+GGG 0.633465 2.3:-55040903 MS.gene67307:CDS
ACCGAAGCACTAAGTGCCGT+CGG 0.645077 2.3:+55040914 None:intergenic
CCTTGTTGTGATGATAACAA+GGG 0.647360 2.3:+55041136 None:intergenic
GGTGTTAGAGTTGACAATGG+CGG 0.648706 2.3:-55040729 MS.gene67307:CDS
GACATACCCGACAACTGCTG+AGG 0.660676 2.3:-55042242 MS.gene67307:intron
CTATAAGATCGGGAATCGAG+AGG 0.665968 2.3:+55041416 None:intergenic
GGCAGGACAAATAGCATGTG+TGG 0.671756 2.3:-55040510 MS.gene67307:CDS
GCCGTGGCTAAAGCTACACG+AGG 0.684997 2.3:+55040977 None:intergenic
TGTTGTGATGATAACAAGGG+AGG 0.686225 2.3:+55041139 None:intergenic
AGATGCGAATCGGATGAAGG+AGG 0.692679 2.3:+55041386 None:intergenic
CCTTGTTATCATCACAACAA+GGG 0.693087 2.3:-55041135 MS.gene67307:CDS
AGAGGTGTTAGAGTTGACAA+TGG 0.702435 2.3:-55040732 MS.gene67307:CDS
TCGTGTAGCTTTAGCCACGG+CGG 0.710232 2.3:-55040975 MS.gene67307:CDS
TAGCAATACCTTGTACACCA+AGG 0.710910 2.3:+55041263 None:intergenic
CCGTGGCTAAAGCTACACGA+GGG 0.768897 2.3:+55040978 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTTTACTCATTTTACT+TGG - chr2.3:55042011-55042030 MS.gene67307:intron 15.0%
!! TTATGATATTGATGAAGAAA+TGG + chr2.3:55040627-55040646 None:intergenic 20.0%
!!! TATATCTTGATTTTGATGAA+GGG + chr2.3:55041155-55041174 None:intergenic 20.0%
!!! TGTTTTTGTTTTGTTTTTTC+AGG - chr2.3:55040793-55040812 MS.gene67307:CDS 20.0%
!!! TTATATCTTGATTTTGATGA+AGG + chr2.3:55041156-55041175 None:intergenic 20.0%
! AACATTAGGAAGAGAAAAAT+GGG - chr2.3:55041840-55041859 MS.gene67307:intron 25.0%
! AAGGTTAAGAAAATGATGAT+AGG + chr2.3:55041325-55041344 None:intergenic 25.0%
! ACTATGAGTATATAGTATGA+GGG + chr2.3:55040465-55040484 None:intergenic 25.0%
! ACTCATAGTATATACTTACA+TGG - chr2.3:55040476-55040495 MS.gene67307:CDS 25.0%
! AGGTTAAGAAAATGATGATA+GGG + chr2.3:55041324-55041343 None:intergenic 25.0%
! ATGAAAAATGTGAAAACCAT+GGG + chr2.3:55040425-55040444 None:intergenic 25.0%
! GAAATGGTATATGAAAACTA+TGG + chr2.3:55040611-55040630 None:intergenic 25.0%
! GCATAGCATATATATTAACA+TGG - chr2.3:55040681-55040700 MS.gene67307:CDS 25.0%
! TACTATGAGTATATAGTATG+AGG + chr2.3:55040466-55040485 None:intergenic 25.0%
! TATGAAAAATGTGAAAACCA+TGG + chr2.3:55040426-55040445 None:intergenic 25.0%
! TGTAGAACATGTATGATAAA+AGG + chr2.3:55041417-55041436 None:intergenic 25.0%
!! TTTTACTCATTTTACTTGGT+GGG - chr2.3:55042015-55042034 MS.gene67307:intron 25.0%
!! TTTTTACTCATTTTACTTGG+TGG - chr2.3:55042014-55042033 MS.gene67307:intron 25.0%
!!! GAAGAAAAGAGTATTGAAAT+TGG + chr2.3:55041395-55041414 None:intergenic 25.0%
!!! TTTTGTTTTGTTTTTTCAGG+TGG - chr2.3:55040796-55040815 MS.gene67307:CDS 25.0%
AAAGGCAAATTTGTCAACTA+TGG - chr2.3:55041768-55041787 MS.gene67307:CDS 30.0%
AACTCACAAACACCAATTAT+AGG + chr2.3:55041919-55041938 None:intergenic 30.0%
ACATTAGGAAGAGAAAAATG+GGG - chr2.3:55041841-55041860 MS.gene67307:intron 30.0%
ATGATCTTAGTTGTTTGCAT+GGG - chr2.3:55040893-55040912 MS.gene67307:CDS 30.0%
CAACATTAGGAAGAGAAAAA+TGG - chr2.3:55041839-55041858 MS.gene67307:intron 30.0%
CTTGCTCACATTTGATTATT+TGG + chr2.3:55042242-55042261 None:intergenic 30.0%
GTTCAACATTAGGGTTATTA+TGG - chr2.3:55041815-55041834 MS.gene67307:intron 30.0%
TTGTGGTATATAACACTGAT+TGG - chr2.3:55042142-55042161 MS.gene67307:intron 30.0%
! TGTGGTATATAACACTGATT+GGG - chr2.3:55042143-55042162 MS.gene67307:intron 30.0%
! TTTTCCATTCAAAGTTGTCT+TGG - chr2.3:55041556-55041575 MS.gene67307:CDS 30.0%
!! CAACTAAGATCATGTTTTTG+AGG + chr2.3:55040886-55040905 None:intergenic 30.0%
!! CTAAAGAAAATGATGGACAT+AGG - chr2.3:55040827-55040846 MS.gene67307:CDS 30.0%
!! TTTACTCATTTTACTTGGTG+GGG - chr2.3:55042016-55042035 MS.gene67307:intron 30.0%
!!! GTTTGTTTTGAAATTAGGGT+TGG - chr2.3:55042062-55042081 MS.gene67307:intron 30.0%
!!! TGGGGTTTGTTTTGAAATTA+GGG - chr2.3:55042058-55042077 MS.gene67307:intron 30.0%
!!! TTGGGGTTTGTTTTGAAATT+AGG - chr2.3:55042057-55042076 MS.gene67307:intron 30.0%
AACTCCAAGACAACTTTGAA+TGG + chr2.3:55041563-55041582 None:intergenic 35.0%
AAGAATGGGGCATGTTAATA+AGG - chr2.3:55041533-55041552 MS.gene67307:CDS 35.0%
ACTCTGTTATTGGAGAAAGA+TGG - chr2.3:55040721-55040740 MS.gene67307:CDS 35.0%
ACTTATGTACACTTTACCGA+CGG - chr2.3:55041687-55041706 MS.gene67307:CDS 35.0%
ATACCATACAAAATCTCCCA+TGG - chr2.3:55040406-55040425 MS.gene67307:CDS 35.0%
ATTAAGCCACAAGAGTGAAA+TGG + chr2.3:55041113-55041132 None:intergenic 35.0%
ATTCCACAACTAGTTGTTTG+TGG + chr2.3:55041952-55041971 None:intergenic 35.0%
CACTAGCAAGTTCAACATTA+GGG - chr2.3:55041806-55041825 MS.gene67307:intron 35.0%
CATGATCTTAGTTGTTTGCA+TGG - chr2.3:55040892-55040911 MS.gene67307:CDS 35.0%
CATTAGGAAGAGAAAAATGG+GGG - chr2.3:55041842-55041861 MS.gene67307:intron 35.0%
CTACGTAAATAGATGCGAAT+CGG + chr2.3:55041244-55041263 None:intergenic 35.0%
CTCTGTTATTGGAGAAAGAT+GGG - chr2.3:55040722-55040741 MS.gene67307:CDS 35.0%
GATAGGGATGTGAATGATAA+CGG + chr2.3:55041308-55041327 None:intergenic 35.0%
GGGTTATTATGGACAACATT+AGG - chr2.3:55041826-55041845 MS.gene67307:intron 35.0%
GGTGTTCACAAATGATAAAG+AGG - chr2.3:55041867-55041886 MS.gene67307:intron 35.0%
GTTGACAAATTTGCCTTTGA+TGG + chr2.3:55041766-55041785 None:intergenic 35.0%
TCACTAGCAAGTTCAACATT+AGG - chr2.3:55041805-55041824 MS.gene67307:CDS 35.0%
TCTGTTATTGGAGAAAGATG+GGG - chr2.3:55040723-55040742 MS.gene67307:CDS 35.0%
TGATGAAGGGATAGCATAAA+TGG + chr2.3:55041142-55041161 None:intergenic 35.0%
TGTGGTATCAATTCTTGTTG+TGG - chr2.3:55042125-55042144 MS.gene67307:intron 35.0%
TGTTATAGCCGACCTTTAAT+CGG - chr2.3:55040562-55040581 MS.gene67307:CDS 35.0%
TTAACCTTCAAACTTCACCT+TGG - chr2.3:55041337-55041356 MS.gene67307:CDS 35.0%
TTAAGCCACAAGAGTGAAAT+GGG + chr2.3:55041112-55041131 None:intergenic 35.0%
TTGTCAACTATGGTAGCAAT+TGG - chr2.3:55041778-55041797 MS.gene67307:CDS 35.0%
! AAACCATGGGAGATTTTGTA+TGG + chr2.3:55040412-55040431 None:intergenic 35.0%
! ATGGCGGTTTTACCTATAAT+TGG - chr2.3:55041904-55041923 MS.gene67307:intron 35.0%
! CCTTGTTATCATCACAACAA+GGG - chr2.3:55041482-55041501 MS.gene67307:CDS 35.0%
! CCTTGTTGTGATGATAACAA+GGG + chr2.3:55041484-55041503 None:intergenic 35.0%
!! AGAGAAGTTTTGTGAACCAA+TGG + chr2.3:55041035-55041054 None:intergenic 35.0%
!! TTACTCATTTTACTTGGTGG+GGG - chr2.3:55042017-55042036 MS.gene67307:intron 35.0%
!!! GTTTTGAAATTAGGGTTGGT+GGG - chr2.3:55042066-55042085 MS.gene67307:intron 35.0%
!!! TGTTTTGAAATTAGGGTTGG+TGG - chr2.3:55042065-55042084 MS.gene67307:intron 35.0%
!!! TGTTTTGTTTTTTCAGGTGG+TGG - chr2.3:55040799-55040818 MS.gene67307:CDS 35.0%
!!! TTTTGAAATTAGGGTTGGTG+GGG - chr2.3:55042067-55042086 MS.gene67307:intron 35.0%
!!! TTTTGTTATGGTTTGTTGGC+AGG - chr2.3:55042090-55042109 MS.gene67307:intron 35.0%
AACCCCAACACTATTCCTAT+TGG + chr2.3:55042045-55042064 None:intergenic 40.0%
AATAGATGCGAATCGGATGA+AGG + chr2.3:55041237-55041256 None:intergenic 40.0%
ACTAGTTGTGGAATGCTTAG+AGG - chr2.3:55041958-55041977 MS.gene67307:intron 40.0%
AGGCTATTCGCTATAAGATC+GGG + chr2.3:55041214-55041233 None:intergenic 40.0%
AGTGAAGTGCACCACAATAA+TGG + chr2.3:55041283-55041302 None:intergenic 40.0%
AGTTGTTTGCATGGGAAAGT+TGG - chr2.3:55040901-55040920 MS.gene67307:CDS 40.0%
ATAGGCTTCACCAATGTAAC+CGG - chr2.3:55040956-55040975 MS.gene67307:CDS 40.0%
ATTGAAGGCAAAAAGCTTGG+TGG - chr2.3:55042176-55042195 MS.gene67307:intron 40.0%
CATTACCCATTTCACTCTTG+TGG - chr2.3:55041104-55041123 MS.gene67307:CDS 40.0%
CGCATCTATTTACGTAGCAA+AGG - chr2.3:55041247-55041266 MS.gene67307:CDS 40.0%
CTAGTTGTGGAATGCTTAGA+GGG - chr2.3:55041959-55041978 MS.gene67307:intron 40.0%
CTTTCTCCAATAACAGAGTG+AGG + chr2.3:55040720-55040739 None:intergenic 40.0%
GAGGCTATTCGCTATAAGAT+CGG + chr2.3:55041215-55041234 None:intergenic 40.0%
GAGTTCATGACAATTCTAGC+TGG - chr2.3:55041604-55041623 MS.gene67307:CDS 40.0%
GGACGAGCTAAAGAAAATGA+TGG - chr2.3:55040820-55040839 MS.gene67307:CDS 40.0%
GTCATTGAAGGCAAAAAGCT+TGG - chr2.3:55042173-55042192 MS.gene67307:intron 40.0%
GTTGTTTGCATGGGAAAGTT+GGG - chr2.3:55040902-55040921 MS.gene67307:CDS 40.0%
TACACCAAGGTGAAGTTTGA+AGG + chr2.3:55041344-55041363 None:intergenic 40.0%
TAGCAATACCTTGTACACCA+AGG + chr2.3:55041357-55041376 None:intergenic 40.0%
TAGTGCTTCGGTTTCAACAA+GGG - chr2.3:55041714-55041733 MS.gene67307:CDS 40.0%
TGTCCACAAACAACTAGTTG+TGG - chr2.3:55041946-55041965 MS.gene67307:intron 40.0%
TGTTGTGATGATAACAAGGG+AGG + chr2.3:55041481-55041500 None:intergenic 40.0%
TTAGTGCTTCGGTTTCAACA+AGG - chr2.3:55041713-55041732 MS.gene67307:CDS 40.0%
TTGAAGGCAAAAAGCTTGGT+GGG - chr2.3:55042177-55042196 MS.gene67307:intron 40.0%
TTGGGAAAGAGAGTCATTGA+AGG - chr2.3:55042161-55042180 MS.gene67307:intron 40.0%
! AAACTTCACCTTGGTGTACA+AGG - chr2.3:55041346-55041365 MS.gene67307:CDS 40.0%
! AGTTGTCGGGTATGTCTTTT+GGG + chr2.3:55040371-55040390 None:intergenic 40.0%
! CCCTTGTTATCATCACAACA+AGG - chr2.3:55041481-55041500 MS.gene67307:CDS 40.0%
! CCCTTGTTGTGATGATAACA+AGG + chr2.3:55041485-55041504 None:intergenic 40.0%
! GTCTTGGAGTTTGCTTAGAA+TGG - chr2.3:55041572-55041591 MS.gene67307:CDS 40.0%
! TTGGAGTTTGCTTAGAATGG+TGG - chr2.3:55041575-55041594 MS.gene67307:CDS 40.0%
!! AGAGGTGTTAGAGTTGACAA+TGG - chr2.3:55041885-55041904 MS.gene67307:intron 40.0%
!! AGCAGTTGATGACATTTGAC+CGG + chr2.3:55040524-55040543 None:intergenic 40.0%
!!! GGGGTTTTGTTATGGTTTGT+TGG - chr2.3:55042086-55042105 MS.gene67307:intron 40.0%
AAAACCCTGGGCAAAGAATG+GGG - chr2.3:55041520-55041539 MS.gene67307:CDS 45.0%
AACAAGGGAGGACTAGGAAT+AGG + chr2.3:55041469-55041488 None:intergenic 45.0%
AACAGAGTGAGGCTGCATAA+AGG + chr2.3:55040709-55040728 None:intergenic 45.0%
AAGCTACACGAGGGTTGTAA+AGG + chr2.3:55041633-55041652 None:intergenic 45.0%
ACCAACAAGTGTCAAAACCC+TGG - chr2.3:55041507-55041526 MS.gene67307:CDS 45.0%
ACCTGAAAGGACTGAGTAAC+CGG + chr2.3:55040978-55040997 None:intergenic 45.0%
ACTATGCTCATGGCTGCTAT+TGG + chr2.3:55040857-55040876 None:intergenic 45.0%
AGGAAGAGAAAAATGGGGGA+AGG - chr2.3:55041846-55041865 MS.gene67307:intron 45.0%
ATAGCAGCCATGAGCATAGT+AGG - chr2.3:55040857-55040876 MS.gene67307:CDS 45.0%
ATAGCCGACCTTTAATCGGA+GGG - chr2.3:55040566-55040585 MS.gene67307:CDS 45.0%
ATGCAGCCTCACTCTGTTAT+TGG - chr2.3:55040711-55040730 MS.gene67307:CDS 45.0%
CAAAACCCTGGGCAAAGAAT+GGG - chr2.3:55041519-55041538 MS.gene67307:CDS 45.0%
CCAACAAGTGTCAAAACCCT+GGG - chr2.3:55041508-55041527 MS.gene67307:CDS 45.0%
CCAATGGCTTGAGTACATAG+AGG + chr2.3:55041019-55041038 None:intergenic 45.0%
CCTCTATGTACTCAAGCCAT+TGG - chr2.3:55041016-55041035 MS.gene67307:CDS 45.0%
CGCCAATAGGAATAGTGTTG+GGG - chr2.3:55042040-55042059 MS.gene67307:intron 45.0%
CTATAAGATCGGGAATCGAG+AGG + chr2.3:55041204-55041223 None:intergenic 45.0%
CTATGCTCATGGCTGCTATT+GGG + chr2.3:55040856-55040875 None:intergenic 45.0%
CTTATGCTCCCTCCGATTAA+AGG + chr2.3:55040577-55040596 None:intergenic 45.0%
CTTCGGTTTCAACAAGGGTA+GGG - chr2.3:55041719-55041738 MS.gene67307:CDS 45.0%
GACTGAGTAACCGGTTACAT+TGG + chr2.3:55040969-55040988 None:intergenic 45.0%
GATGATAACAAGGGAGGACT+AGG + chr2.3:55041475-55041494 None:intergenic 45.0%
GCACAACTTGGCCATTATTG+TGG - chr2.3:55041269-55041288 MS.gene67307:CDS 45.0%
GCGCCAATAGGAATAGTGTT+GGG - chr2.3:55042039-55042058 MS.gene67307:intron 45.0%
GGTGTTAGAGTTGACAATGG+CGG - chr2.3:55041888-55041907 MS.gene67307:intron 45.0%
GGTTACATTGGTGAAGCCTA+TGG + chr2.3:55040957-55040976 None:intergenic 45.0%
TATAGCCGACCTTTAATCGG+AGG - chr2.3:55040565-55040584 MS.gene67307:CDS 45.0%
TATCGTACCTCAGCAGTTGT+CGG + chr2.3:55040385-55040404 None:intergenic 45.0%
TCAAAACCCTGGGCAAAGAA+TGG - chr2.3:55041518-55041537 MS.gene67307:CDS 45.0%
TGACATTTGACCGGAGAAAG+AGG + chr2.3:55040515-55040534 None:intergenic 45.0%
TGAGTTAGCAAGTGGTGCTT+TGG - chr2.3:55040931-55040950 MS.gene67307:CDS 45.0%
TTGTATTACTATTCCCGCCG+TGG + chr2.3:55041659-55041678 None:intergenic 45.0%
! AAGCACCACTTGCTAACTCA+AGG + chr2.3:55040931-55040950 None:intergenic 45.0%
! CAGTTGTCGGGTATGTCTTT+TGG + chr2.3:55040372-55040391 None:intergenic 45.0%
! GAGGTATCCTACTATGCTCA+TGG + chr2.3:55040867-55040886 None:intergenic 45.0%
! GAGTTTGCTTAGAATGGTGG+TGG - chr2.3:55041578-55041597 MS.gene67307:CDS 45.0%
! TACGTAGCAAAGGCACAACT+TGG - chr2.3:55041257-55041276 MS.gene67307:CDS 45.0%
ACAAGGGTAGGGAATGCACT+TGG - chr2.3:55041730-55041749 MS.gene67307:CDS 50.0%
ACATAGAGGCTCCATTCCCA+TGG + chr2.3:55041005-55041024 None:intergenic 50.0%
ACCGGTTACTCAGTCCTTTC+AGG - chr2.3:55040974-55040993 MS.gene67307:CDS 50.0%
AGATGCGAATCGGATGAAGG+AGG + chr2.3:55041234-55041253 None:intergenic 50.0%
ATCGTACCTCAGCAGTTGTC+GGG + chr2.3:55040384-55040403 None:intergenic 50.0%
CATTTGACCGGAGAAAGAGG+CGG + chr2.3:55040512-55040531 None:intergenic 50.0%
GAATCGAGAGGCGACAATAG+AGG + chr2.3:55041192-55041211 None:intergenic 50.0%
GCTTCGGTTTCAACAAGGGT+AGG - chr2.3:55041718-55041737 MS.gene67307:CDS 50.0%
GGCAGGACAAATAGCATGTG+TGG - chr2.3:55042107-55042126 MS.gene67307:intron 50.0%
GGCGCCAATAGGAATAGTGT+TGG - chr2.3:55042038-55042057 MS.gene67307:intron 50.0%
GGGAGGACTAGGAATAGGTA+TGG + chr2.3:55041464-55041483 None:intergenic 50.0%
TGCTCCCTCCGATTAAAGGT+CGG + chr2.3:55040573-55040592 None:intergenic 50.0%
TGGAGCTGGTCAACCATCAA+AGG - chr2.3:55041750-55041769 MS.gene67307:CDS 50.0%
TTCAGGTCTAGCCATGGGAA+TGG - chr2.3:55040991-55041010 MS.gene67307:CDS 50.0%
TTCCCATGGCTAGACCTGAA+AGG + chr2.3:55040991-55041010 None:intergenic 50.0%
TTTATCCCTGCCCCAATACC+TGG + chr2.3:55041994-55042013 None:intergenic 50.0%
! AGTCCTTTCAGGTCTAGCCA+TGG - chr2.3:55040985-55041004 MS.gene67307:CDS 50.0%
! CTTTTTGCCGCCTCTTTCTC+CGG - chr2.3:55040502-55040521 MS.gene67307:CDS 50.0%
! GAATGCTTAGAGGGAGTGCA+AGG - chr2.3:55041968-55041987 MS.gene67307:intron 50.0%
! GGTAGCCTTGAGTTAGCAAG+TGG - chr2.3:55040923-55040942 MS.gene67307:CDS 50.0%
! GTCCTTTCAGGTCTAGCCAT+GGG - chr2.3:55040986-55041005 MS.gene67307:CDS 50.0%
!! CCCAGGGTTTTGACACTTGT+TGG + chr2.3:55041511-55041530 None:intergenic 50.0%
!!! GGGTTGGTGGGGTTTTGTTA+TGG - chr2.3:55042078-55042097 MS.gene67307:intron 50.0%
ACATGCCCCATTCTTTGCCC+AGG + chr2.3:55041528-55041547 None:intergenic 55.0%
ACCGACGGCACTTAGTGCTT+CGG - chr2.3:55041702-55041721 MS.gene67307:CDS 55.0%
CATGCCCCATTCTTTGCCCA+GGG + chr2.3:55041527-55041546 None:intergenic 55.0%
CCGTGGCTAAAGCTACACGA+GGG + chr2.3:55041642-55041661 None:intergenic 55.0%
CGGAGAAAGAGGCGGCAAAA+AGG + chr2.3:55040504-55040523 None:intergenic 55.0%
GACATACCCGACAACTGCTG+AGG - chr2.3:55040375-55040394 MS.gene67307:CDS 55.0%
GCAAGTGGTGCTTTGGCCAT+AGG - chr2.3:55040938-55040957 MS.gene67307:CDS 55.0%
GGAGTGCAAGGCCAGGTATT+GGG - chr2.3:55041980-55041999 MS.gene67307:intron 55.0%
GGTCGGCTATAACAGCTCTC+TGG + chr2.3:55040556-55040575 None:intergenic 55.0%
GTAGGGAATGCACTTGGAGC+TGG - chr2.3:55041736-55041755 MS.gene67307:CDS 55.0%
! ACCGAAGCACTAAGTGCCGT+CGG + chr2.3:55041706-55041725 None:intergenic 55.0%
! GAGTGCAAGGCCAGGTATTG+GGG - chr2.3:55041981-55042000 MS.gene67307:intron 55.0%
!! CCCTCGTGTAGCTTTAGCCA+CGG - chr2.3:55041639-55041658 MS.gene67307:CDS 55.0%
!! TCGTGTAGCTTTAGCCACGG+CGG - chr2.3:55041642-55041661 MS.gene67307:CDS 55.0%
GCCGTGGCTAAAGCTACACG+AGG + chr2.3:55041643-55041662 None:intergenic 60.0%
! CTTAGAGGGAGTGCAAGGCC+AGG - chr2.3:55041973-55041992 MS.gene67307:intron 60.0%
! GGGAGTGCAAGGCCAGGTAT+TGG - chr2.3:55041979-55041998 MS.gene67307:intron 60.0%
!! CAAGGCCAGGTATTGGGGCA+GGG - chr2.3:55041986-55042005 MS.gene67307:intron 60.0%
!! CGTGTAGCTTTAGCCACGGC+GGG - chr2.3:55041643-55041662 MS.gene67307:CDS 60.0%
!! TACTTGGTGGGGGCGCCAAT+AGG - chr2.3:55042027-55042046 MS.gene67307:intron 60.0%
!! GCAAGGCCAGGTATTGGGGC+AGG - chr2.3:55041985-55042004 MS.gene67307:intron 65.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 55040349 55042290 55040349 ID=MS.gene67307
chr2.3 mRNA 55040349 55042290 55040349 ID=MS.gene67307.t1;Parent=MS.gene67307
chr2.3 exon 55042243 55042290 55042243 ID=MS.gene67307.t1.exon1;Parent=MS.gene67307.t1
chr2.3 CDS 55042243 55042290 55042243 ID=cds.MS.gene67307.t1;Parent=MS.gene67307.t1
chr2.3 exon 55040349 55041824 55040349 ID=MS.gene67307.t1.exon2;Parent=MS.gene67307.t1
chr2.3 CDS 55040349 55041824 55040349 ID=cds.MS.gene67307.t1;Parent=MS.gene67307.t1
Gene Sequence

>MS.gene67307

ATGGTTGCTCAAGAGAAATCCCAAAAGACATACCCGACAACTGCTGAGGTACGATATATACCATACAAAATCTCCCATGGTTTTCACATTTTTCATACATATCACTTCTTCATCCCTCATACTATATACTCATAGTATATACTTACATGGATCCTTTTTGCCGCCTCTTTCTCCGGTCAAATGTCATCAACTGCTGACATTTGACCAGAGAGCTGTTATAGCCGACCTTTAATCGGAGGGAGCATAAGATATTAAAATTCCATAGTTTTCATATACCATTTCTTCATCAATATCATAACATAGCATTAGTTCAACAAAAAAAAATTATCATAGCATAGCATATATATTAACATGGATCCTTTATGCAGCCTCACTCTGTTATTGGAGAAAGATGGGGCTGTTGAGAAGCTATGTCTTATAGTATATACTTGTAAAAGTTCATATTGTTTTTGTTTTGTTTTTTCAGGTGGTGGACGAGCTAAAGAAAATGATGGACATAGGTTTCCCAATAGCAGCCATGAGCATAGTAGGATACCTCAAAAACATGATCTTAGTTGTTTGCATGGGAAAGTTGGGTAGCCTTGAGTTAGCAAGTGGTGCTTTGGCCATAGGCTTCACCAATGTAACCGGTTACTCAGTCCTTTCAGGTCTAGCCATGGGAATGGAGCCTCTATGTACTCAAGCCATTGGTTCACAAAACTTCTCTTTAGTCTCTCTCATATTAAGAAGAACAATTCTCATGTTATTAGTAGCTTCATTACCCATTTCACTCTTGTGGCTTAATCTTGAACCATTTATGCTATCCCTTCATCAAAATCAAGATATAACAAGAATAGCAAGCCTCTATTGTCGCCTCTCGATTCCCGATCTTATAGCGAATAGCCTCCTTCATCCGATTCGCATCTATTTACGTAGCAAAGGCACAACTTGGCCATTATTGTGGTGCACTTCACTTTCCGTTATCATTCACATCCCTATCATCATTTTCTTAACCTTCAAACTTCACCTTGGTGTACAAGGTATTGCTATTTCAGCTTTTGTTGCCAATTTCAATACTCTTTTCTTCCTTTTATCATACATGTTCTACATGCATGTCTCACATGTTTCAATCTCCATACCTATTCCTAGTCCTCCCTTGTTATCATCACAACAAGGGAAACCAACAAGTGTCAAAACCCTGGGCAAAGAATGGGGCATGTTAATAAGGTTTTCCATTCAAAGTTGTCTTGGAGTTTGCTTAGAATGGTGGTGGTATGAGTTCATGACAATTCTAGCTGGTTACCTTTACAACCCTCGTGTAGCTTTAGCCACGGCGGGAATAGTAATACAAACAACATCACTTATGTACACTTTACCGACGGCACTTAGTGCTTCGGTTTCAACAAGGGTAGGGAATGCACTTGGAGCTGGTCAACCATCAAAGGCAAATTTGTCAACTATGGTAGCAATTGGAATGTCACTAGCAAGTTCAACATTAGGGTTATTATGGACAACATTAGGAAGAGAAAAATGGGGGAAGGTGTTCACAAATGATAAAGAGGTGTTAGAGTTGACAATGGCGGTTTTACCTATAATTGGTGTTTGTGAGTTAGCAAATTGTCCACAAACAACTAGTTGTGGAATGCTTAGAGGGAGTGCAAGGCCAGGTATTGGGGCAGGGATAAATTTTTACTCATTTTACTTGGTGGGGGCGCCAATAGGAATAGTGTTGGGGTTTGTTTTGAAATTAGGGTTGGTGGGGTTTTGTTATGGTTTGTTGGCAGGACAAATAGCATGTGTGGTATCAATTCTTGTTGTGGTATATAACACTGATTGGGAAAGAGAGTCATTGAAGGCAAAAAGCTTGGTGGGTAATGATACATGTGACACATTATTTGCTCATGTTGAAGACCAAATAATCAAATGTGAGCAAGATATTGTCTTTCTCAATGAGAACAAGTGA
Protein sequence

>MS.gene67307.t1

MVAQEKSQKTYPTTAEVVDELKKMMDIGFPIAAMSIVGYLKNMILVVCMGKLGSLELASGALAIGFTNVTGYSVLSGLAMGMEPLCTQAIGSQNFSLVSLILRRTILMLLVASLPISLLWLNLEPFMLSLHQNQDITRIASLYCRLSIPDLIANSLLHPIRIYLRSKGTTWPLLWCTSLSVIIHIPIIIFLTFKLHLGVQGIAISAFVANFNTLFFLLSYMFYMHVSHVSISIPIPSPPLLSSQQGKPTSVKTLGKEWGMLIRFSIQSCLGVCLEWWWYEFMTILAGYLYNPRVALATAGIVIQTTSLMYTLPTALSASVSTRVGNALGAGQPSKANLSTMVAIGMSLASSTLGLLWTTLGREKWGKVFTNDKEVLELTMAVLPIIGVCELANCPQTTSCGMLRGSARPGIGAGINFYSFYLVGAPIGIVLGFVLKLGLVGFCYGLLAGQIACVVSILVVVYNTDWERESLKAKSLVGNDTCDTLFAHVEDQIIKCEQDIVFLNENK