AlfalfaGEDB | MS.gene67611

M. sativa cultivar XinJiangDaYe / MS.gene67611

KEGG: K08066
GO: GO:0003674 GO:0005488 GO:0005515 GO:0046982 GO:0046983
PFAM: PF00125.27

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67611.t1	ABD32395.1	95.3	150	7	0	1	150	100	249	2.80E-75	291.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67611.t1	Q9ZVL3	64.6	147	40	4	1	146	80	215	6.5e-41	168.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67611.t1	I3TAX9	95.3	150	7	0	1	150	111	260	2.0e-75	291.2

TFs/TRs:

Gene ID	Type	Classification
MS.gene67611.t1	TF	NF-YC

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050859	MS.gene67611	0.949076	2.64E-107	-1.69E-46
MS.gene050860	MS.gene67611	0.942549	5.91E-102	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67611.t1	MTR_7g113680	95.333	150	7	0	1	150	162	311	4.63e-104	300
MS.gene67611.t1	MTR_1g082660	80.451	133	22	1	1	133	111	239	1.12e-73	221
MS.gene67611.t1	MTR_3g099180	65.574	122	29	3	1	121	65	174	1.11e-44	146
MS.gene67611.t1	MTR_3g099180	65.574	122	29	3	1	121	65	174	1.28e-44	145
MS.gene67611.t1	MTR_2g023340	44.654	159	63	7	1	149	95	238	2.15e-36	125
MS.gene67611.t1	MTR_2g081600	52.703	74	35	0	2	75	34	107	2.48e-23	88.6
MS.gene67611.t1	MTR_2g081630	54.286	70	32	0	2	71	34	103	7.08e-23	87.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67611.t1	AT1G08970	71.533	137	32	3	1	137	90	219	3.47e-62	191
MS.gene67611.t1	AT1G08970	71.533	137	32	3	1	137	90	219	3.47e-62	191
MS.gene67611.t1	AT1G08970	71.533	137	32	3	1	137	90	219	3.47e-62	191
MS.gene67611.t1	AT1G08970	71.533	137	32	3	1	137	90	219	3.47e-62	191
MS.gene67611.t1	AT1G54830	68.027	147	35	4	1	146	80	215	4.89e-61	187
MS.gene67611.t1	AT1G54830	68.027	147	35	4	1	146	80	215	4.89e-61	187
MS.gene67611.t1	AT1G54830	68.027	147	35	4	1	146	80	215	4.89e-61	187
MS.gene67611.t1	AT3G48590	66.935	124	37	2	1	124	75	194	5.19e-52	165
MS.gene67611.t1	AT5G63470	66.142	127	40	3	1	126	88	212	3.44e-49	158
MS.gene67611.t1	AT5G63470	66.142	127	40	3	1	126	88	212	3.44e-49	158
MS.gene67611.t1	AT1G56170	71.296	108	24	3	1	106	86	188	4.09e-47	151
MS.gene67611.t1	AT1G56170	71.296	108	24	3	1	106	86	188	4.09e-47	151
MS.gene67611.t1	AT5G50480	45.324	139	55	4	1	138	64	182	9.87e-32	112
MS.gene67611.t1	AT5G27910	41.259	143	55	6	1	138	46	164	6.13e-23	89.7
MS.gene67611.t1	AT5G50490	38.129	139	75	3	1	139	46	173	2.95e-22	87.8
MS.gene67611.t1	AT5G50470	51.316	76	31	1	1	70	73	148	1.46e-20	84.0
MS.gene67611.t1	AT5G38140	45.588	68	37	0	1	68	77	144	2.96e-17	75.1

Find 52 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGCCAGCACTTCATCTTTC+AGG	0.158704	7.3:+3343154	None:intergenic
GACTGATATCTTTGATTTCC+TGG	0.246183	7.3:-3343199	MS.gene67611:CDS
GGAGATGGTGGTCGCTGTTC+CGG	0.343092	7.3:+3342946	None:intergenic
GCTGCTGAGCATACATGTTT+GGG	0.352203	7.3:+3343006	None:intergenic
GCCTCCCAAGTTGGAACTGC+TGG	0.356093	7.3:-3343057	MS.gene67611:CDS
GGCTGCTGAGCATACATGTT+TGG	0.368122	7.3:+3343005	None:intergenic
TGGAACTGCTGGTGTCATAA+TGG	0.411463	7.3:-3343046	MS.gene67611:CDS
AGAGGAACAATGCCTGTTGC+TGG	0.417994	7.3:-3343123	MS.gene67611:CDS
CATCTCAGCATCTAGTGATC+TGG	0.437549	7.3:+3342925	None:intergenic
CAGTGTGGTTCCATGAGCGC+AGG	0.449372	7.3:+3343277	None:intergenic
ACAAATGTGGCCACAGCCTC+CGG	0.450073	7.3:-3342965	MS.gene67611:CDS
GCATCAGCAGGACCAGCAAC+AGG	0.455014	7.3:+3343111	None:intergenic
GGCCTGTGAGATGTTCATAT+TGG	0.456864	7.3:-3343307	MS.gene67611:CDS
TGGAGTTAACCCTGCGCTCA+TGG	0.458619	7.3:-3343287	MS.gene67611:CDS
CACATTTGTGGTGCCATGTA+GGG	0.465255	7.3:+3342979	None:intergenic
TGACACCAGCAGTTCCAACT+TGG	0.478083	7.3:+3343052	None:intergenic
GGAACTGCTGGTGTCATAAT+GGG	0.479046	7.3:-3343045	MS.gene67611:CDS
ACATGTTTGGGGTCCATCAC+AGG	0.484477	7.3:+3343018	None:intergenic
GGAGGCTGTGGCCACATTTG+TGG	0.486993	7.3:+3342967	None:intergenic
GCAACAGGCATTGTTCCTCT+TGG	0.487666	7.3:+3343126	None:intergenic
ACCACACTGAAGAGAACAAA+AGG	0.492883	7.3:-3343263	MS.gene67611:CDS
CCACATTTGTGGTGCCATGT+AGG	0.497501	7.3:+3342978	None:intergenic
CCTCAGCATGCCTCCCAAGT+TGG	0.514710	7.3:-3343066	MS.gene67611:CDS
CTCAGCAGCCCATCCCTACA+TGG	0.522180	7.3:-3342992	MS.gene67611:CDS
TTTGTGGTGCCATGTAGGGA+TGG	0.525417	7.3:+3342983	None:intergenic
GGTCGCTGTTCCGGAGGCTG+TGG	0.539229	7.3:+3342955	None:intergenic
GGACCTGAAAGATGAAGTGC+TGG	0.542953	7.3:-3343157	MS.gene67611:CDS
ACTTGGGAGGCATGCTGAGG+TGG	0.548128	7.3:+3343069	None:intergenic
GACACCAGCAGTTCCAACTT+GGG	0.552393	7.3:+3343053	None:intergenic
AAGCACCTGTCATATTTGCA+AGG	0.553287	7.3:-3343329	MS.gene67611:CDS
CAGTAAGGAAGAGCATCAGC+AGG	0.560205	7.3:+3343099	None:intergenic
CAGGCCCTTGCAAATATGAC+AGG	0.560856	7.3:+3343324	None:intergenic
AGCATCTAGTGATCTGGAGA+TGG	0.574668	7.3:+3342931	None:intergenic
ATATTGCTGCTGCAATCACT+AGG	0.575052	7.3:-3343221	MS.gene67611:CDS
GAAGATCATGAAGGCTGATG+AGG	0.575398	7.3:-3343373	None:intergenic
TGAGGTGGCATGTAGCAGTA+AGG	0.584291	7.3:+3343084	None:intergenic
CATAATGGGAAAGCCTGTGA+TGG	0.593370	7.3:-3343031	MS.gene67611:CDS
CACGAGGCACAATGTCAACC+AGG	0.593690	7.3:+3343181	None:intergenic
TTGTGGTGCCATGTAGGGAT+GGG	0.603796	7.3:+3342984	None:intergenic
ATCTAGTGATCTGGAGATGG+TGG	0.605869	7.3:+3342934	None:intergenic
CTCCAATATGAACATCTCAC+AGG	0.609517	7.3:+3343305	None:intergenic
AGCACCTGTCATATTTGCAA+GGG	0.622434	7.3:-3343328	MS.gene67611:CDS
ACCAGCAGTTCCAACTTGGG+AGG	0.637100	7.3:+3343056	None:intergenic
AGTGTGGTTCCATGAGCGCA+GGG	0.637592	7.3:+3343278	None:intergenic
CCTACATGGCACCACAAATG+TGG	0.644640	7.3:-3342978	MS.gene67611:CDS
GATGGTGGTCGCTGTTCCGG+AGG	0.649506	7.3:+3342949	None:intergenic
CCAACTTGGGAGGCATGCTG+AGG	0.657795	7.3:+3343066	None:intergenic
TCATCTTTCAGGTCCTCACG+AGG	0.665344	7.3:+3343165	None:intergenic
GGTTGACATTGTGCCTCGTG+AGG	0.672349	7.3:-3343178	MS.gene67611:CDS
CTGCTGAGCATACATGTTTG+GGG	0.676930	7.3:+3343007	None:intergenic
ACACTGAAGAGAACAAAAGG+AGG	0.682027	7.3:-3343260	MS.gene67611:CDS
GTGCTGGCTTCAATTCCAAG+AGG	0.714242	7.3:-3343141	MS.gene67611:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	GACTGATATCTTTGATTTCC+TGG	-	chr7.3:3343103-3343122	MS.gene67611:CDS	35.0%
	AAGCACCTGTCATATTTGCA+AGG	-	chr7.3:3342973-3342992	MS.gene67611:CDS	40.0%
	ACACTGAAGAGAACAAAAGG+AGG	-	chr7.3:3343042-3343061	MS.gene67611:CDS	40.0%
	ACCACACTGAAGAGAACAAA+AGG	-	chr7.3:3343039-3343058	MS.gene67611:CDS	40.0%
	AGCACCTGTCATATTTGCAA+GGG	-	chr7.3:3342974-3342993	MS.gene67611:CDS	40.0%
	ATATTGCTGCTGCAATCACT+AGG	-	chr7.3:3343081-3343100	MS.gene67611:CDS	40.0%
	CTCCAATATGAACATCTCAC+AGG	+	chr7.3:3343000-3343019	None:intergenic	40.0%
!	TCCTTTTGTTCTCTTCAGTG+TGG	+	chr7.3:3343043-3343062	None:intergenic	40.0%
	AAGCCAGCACTTCATCTTTC+AGG	+	chr7.3:3343151-3343170	None:intergenic	45.0%
	CTGCTGAGCATACATGTTTG+GGG	+	chr7.3:3343298-3343317	None:intergenic	45.0%
	GCTGCTGAGCATACATGTTT+GGG	+	chr7.3:3343299-3343318	None:intergenic	45.0%
	GGCCTGTGAGATGTTCATAT+TGG	-	chr7.3:3342995-3343014	MS.gene67611:CDS	45.0%
!	CATAATGGGAAAGCCTGTGA+TGG	-	chr7.3:3343271-3343290	MS.gene67611:CDS	45.0%
!!	CACATTTGTGGTGCCATGTA+GGG	+	chr7.3:3343326-3343345	None:intergenic	45.0%
!!	GGAACTGCTGGTGTCATAAT+GGG	-	chr7.3:3343257-3343276	MS.gene67611:CDS	45.0%
!!	TGGAACTGCTGGTGTCATAA+TGG	-	chr7.3:3343256-3343275	MS.gene67611:CDS	45.0%
	AGAGGAACAATGCCTGTTGC+TGG	-	chr7.3:3343179-3343198	MS.gene67611:CDS	50.0%
	CAGGCCCTTGCAAATATGAC+AGG	+	chr7.3:3342981-3343000	None:intergenic	50.0%
	CCTACATGGCACCACAAATG+TGG	-	chr7.3:3343324-3343343	MS.gene67611:CDS	50.0%
	GACACCAGCAGTTCCAACTT+GGG	+	chr7.3:3343252-3343271	None:intergenic	50.0%
	GGACCTGAAAGATGAAGTGC+TGG	-	chr7.3:3343145-3343164	MS.gene67611:CDS	50.0%
	GGCTGCTGAGCATACATGTT+TGG	+	chr7.3:3343300-3343319	None:intergenic	50.0%
	TGACACCAGCAGTTCCAACT+TGG	+	chr7.3:3343253-3343272	None:intergenic	50.0%
	TGAGGTGGCATGTAGCAGTA+AGG	+	chr7.3:3343221-3343240	None:intergenic	50.0%
!	ACATGTTTGGGGTCCATCAC+AGG	+	chr7.3:3343287-3343306	None:intergenic	50.0%
!	GCAACAGGCATTGTTCCTCT+TGG	+	chr7.3:3343179-3343198	None:intergenic	50.0%
!	TCATCTTTCAGGTCCTCACG+AGG	+	chr7.3:3343140-3343159	None:intergenic	50.0%
!!	CAGTAAGGAAGAGCATCAGC+AGG	+	chr7.3:3343206-3343225	None:intergenic	50.0%
!!	CCACATTTGTGGTGCCATGT+AGG	+	chr7.3:3343327-3343346	None:intergenic	50.0%
!!	GTGCTGGCTTCAATTCCAAG+AGG	-	chr7.3:3343161-3343180	MS.gene67611:CDS	50.0%
!!	TTGTGGTGCCATGTAGGGAT+GGG	+	chr7.3:3343321-3343340	None:intergenic	50.0%
!!	TTTGTGGTGCCATGTAGGGA+TGG	+	chr7.3:3343322-3343341	None:intergenic	50.0%
	ACAAATGTGGCCACAGCCTC+CGG	-	chr7.3:3343337-3343356	MS.gene67611:CDS	55.0%
	ACCAGCAGTTCCAACTTGGG+AGG	+	chr7.3:3343249-3343268	None:intergenic	55.0%
	AGTGTGGTTCCATGAGCGCA+GGG	+	chr7.3:3343027-3343046	None:intergenic	55.0%
	CACGAGGCACAATGTCAACC+AGG	+	chr7.3:3343124-3343143	None:intergenic	55.0%
	GGTTGACATTGTGCCTCGTG+AGG	-	chr7.3:3343124-3343143	MS.gene67611:CDS	55.0%
	TGGAGTTAACCCTGCGCTCA+TGG	-	chr7.3:3343015-3343034	MS.gene67611:CDS	55.0%
	CAGTGTGGTTCCATGAGCGC+AGG	+	chr7.3:3343028-3343047	None:intergenic	60.0%
	CCTCAGCATGCCTCCCAAGT+TGG	-	chr7.3:3343236-3343255	MS.gene67611:CDS	60.0%
	CTCAGCAGCCCATCCCTACA+TGG	-	chr7.3:3343310-3343329	MS.gene67611:CDS	60.0%
	GCATCAGCAGGACCAGCAAC+AGG	+	chr7.3:3343194-3343213	None:intergenic	60.0%
	GGAGGCTGTGGCCACATTTG+TGG	+	chr7.3:3343338-3343357	None:intergenic	60.0%
!	ACTTGGGAGGCATGCTGAGG+TGG	+	chr7.3:3343236-3343255	None:intergenic	60.0%
!	CCAACTTGGGAGGCATGCTG+AGG	+	chr7.3:3343239-3343258	None:intergenic	60.0%
!	GCCTCCCAAGTTGGAACTGC+TGG	-	chr7.3:3343245-3343264	MS.gene67611:CDS	60.0%
!	GGAGATGGTGGTCGCTGTTC+CGG	+	chr7.3:3343359-3343378	None:intergenic	60.0%
!	GATGGTGGTCGCTGTTCCGG+AGG	+	chr7.3:3343356-3343375	None:intergenic	65.0%
	GGTCGCTGTTCCGGAGGCTG+TGG	+	chr7.3:3343350-3343369	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	3342936	3343388	3342936	ID=MS.gene67611
chr7.3	mRNA	3342936	3343388	3342936	ID=MS.gene67611.t1;Parent=MS.gene67611
chr7.3	exon	3342936	3343388	3342936	ID=MS.gene67611.t1.exon1;Parent=MS.gene67611.t1
chr7.3	CDS	3342936	3343388	3342936	ID=cds.MS.gene67611.t1;Parent=MS.gene67611.t1

Gene Sequence

>MS.gene67611

ATGAAGGCTGATGAGGATGTTAGAATGATATCAGCTGAAGCACCTGTCATATTTGCAAGGGCCTGTGAGATGTTCATATTGGAGTTAACCCTGCGCTCATGGAACCACACTGAAGAGAACAAAAGGAGGACACTTCAGAAGAATGATATTGCTGCTGCAATCACTAGGACTGATATCTTTGATTTCCTGGTTGACATTGTGCCTCGTGAGGACCTGAAAGATGAAGTGCTGGCTTCAATTCCAAGAGGAACAATGCCTGTTGCTGGTCCTGCTGATGCTCTTCCTTACTGCTACATGCCACCTCAGCATGCCTCCCAAGTTGGAACTGCTGGTGTCATAATGGGAAAGCCTGTGATGGACCCCAAACATGTATGCTCAGCAGCCCATCCCTACATGGCACCACAAATGTGGCCACAGCCTCCGGAACAGCGACCACCATCTCCAGATCACTAG

Protein sequence

>MS.gene67611.t1

MKADEDVRMISAEAPVIFARACEMFILELTLRSWNHTEENKRRTLQKNDIAAAITRTDIFDFLVDIVPREDLKDEVLASIPRGTMPVAGPADALPYCYMPPQHASQVGTAGVIMGKPVMDPKHVCSAAHPYMAPQMWPQPPEQRPPSPDH