AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene67647

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67647.t1 MTR_2g450310 92.000 375 30 0 1 375 1 375 0.0 699
MS.gene67647.t1 MTR_2g450510 78.667 375 80 0 1 375 1 375 0.0 605
MS.gene67647.t1 MTR_4g124800 42.936 361 186 7 27 376 36 387 1.98e-94 292
MS.gene67647.t1 MTR_2g020630 39.945 363 212 4 1 360 10 369 4.14e-93 288
MS.gene67647.t1 MTR_2g044140 41.755 376 212 5 4 376 13 384 2.15e-91 284
MS.gene67647.t1 MTR_1088s0010 82.993 147 25 0 1 147 1 147 4.24e-89 265
MS.gene67647.t1 MTR_2g043850 42.287 376 210 5 4 376 13 384 3.75e-88 276
MS.gene67647.t1 MTR_1308s0010 77.852 149 33 0 227 375 1 149 2.96e-80 244
MS.gene67647.t1 MTR_1372s0010 81.013 79 15 0 147 225 1 79 7.72e-41 139
MS.gene67647.t1 MTR_1g069275 26.471 374 207 18 7 374 27 338 1.38e-16 81.3
MS.gene67647.t1 MTR_7g107710 25.722 381 209 19 2 374 3 317 4.24e-15 76.3
MS.gene67647.t1 MTR_1g011630 26.064 376 203 16 7 374 18 326 4.78e-15 76.3
MS.gene67647.t1 MTR_8g037880 25.692 253 131 7 4 232 3 222 5.02e-15 76.6
MS.gene67647.t1 MTR_8g037910 25.692 253 130 7 4 232 3 221 7.73e-15 74.3
MS.gene67647.t1 MTR_8g037910 25.692 253 130 7 4 232 3 221 1.11e-14 74.7
MS.gene67647.t1 MTR_8g037910 25.692 253 130 7 4 232 3 221 1.80e-14 74.7
MS.gene67647.t1 MTR_7g099330 25.201 373 209 18 7 374 26 333 2.06e-14 74.7
MS.gene67647.t1 MTR_8g432640 25.849 383 205 18 2 374 3 316 1.57e-13 71.6
MS.gene67647.t1 MTR_4g051642 23.822 382 220 17 1 376 3 319 4.02e-13 70.5
MS.gene67647.t1 MTR_5g033260 24.352 386 207 16 2 374 3 316 7.15e-13 69.7
MS.gene67647.t1 MTR_3g109520 27.005 374 206 18 7 374 41 353 4.72e-12 67.4
MS.gene67647.t1 MTR_3g437580 27.165 254 132 11 3 229 11 238 5.62e-12 67.4
MS.gene67647.t1 MTR_3g437580 27.165 254 132 11 3 229 11 238 6.57e-12 66.6
MS.gene67647.t1 MTR_7g107690 23.421 380 223 18 1 374 1 318 1.28e-11 65.9
MS.gene67647.t1 MTR_1g008380 25.000 376 210 20 7 374 44 355 6.31e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67647.t1 AT1G19250 43.570 381 198 8 4 376 12 383 2.65e-102 312
MS.gene67647.t1 AT5G45180 38.889 378 178 10 4 375 12 342 1.08e-82 259
MS.gene67647.t1 AT4G28720 27.200 375 206 20 7 374 27 341 2.67e-17 83.2
MS.gene67647.t1 AT1G62560 26.772 254 127 9 3 229 11 232 1.36e-15 78.2
MS.gene67647.t1 AT1G62560 26.772 254 127 9 3 229 11 232 1.83e-15 78.2
MS.gene67647.t1 AT1G48910 25.661 378 214 16 1 374 1 315 4.93e-15 76.3
MS.gene67647.t1 AT5G43890 26.738 374 209 19 7 374 27 341 5.49e-15 76.3
MS.gene67647.t1 AT4G32540 26.330 376 204 18 7 374 23 333 3.80e-14 73.9
MS.gene67647.t1 AT5G11320 26.121 379 197 16 7 374 19 325 4.69e-14 73.6
MS.gene67647.t1 AT5G07800 26.154 260 128 9 3 229 14 242 5.83e-14 73.6
MS.gene67647.t1 AT5G11320 25.984 381 199 16 7 376 19 327 6.69e-14 72.8
MS.gene67647.t1 AT4G32540 27.823 248 133 12 7 251 23 227 8.81e-14 72.8
MS.gene67647.t1 AT1G65860 23.622 254 134 7 3 229 11 231 2.57e-13 71.2
MS.gene67647.t1 AT1G62620 25.896 251 132 8 5 229 13 235 4.52e-13 70.5
MS.gene67647.t1 AT1G62620 25.896 251 132 8 5 229 13 235 6.49e-13 70.1
MS.gene67647.t1 AT1G04610 27.128 376 206 17 7 374 39 354 9.50e-13 69.7
MS.gene67647.t1 AT1G63370 25.498 251 133 8 5 229 13 235 1.69e-12 68.2
MS.gene67647.t1 AT1G63370 25.498 251 133 8 5 229 13 235 2.00e-12 68.6
MS.gene67647.t1 AT1G21430 26.005 373 209 17 5 374 9 317 2.32e-12 68.2
MS.gene67647.t1 AT4G13260 23.760 383 207 17 7 374 30 342 5.15e-12 67.4
MS.gene67647.t1 AT4G13260 24.242 297 171 13 7 288 30 287 9.50e-12 65.9

Find 67 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCCTAGATTTCAACATTT+TGG 0.064568 2.2:-34854212 MS.gene67647:CDS
AATAACTACATCTGTTTCTA+AGG 0.224263 2.2:+34853738 None:intergenic
CCCGGAGAACACTTCCTTCT+TGG 0.233331 2.2:-34854133 MS.gene67647:CDS
ACACTTCCTTCTTGGCCTTT+TGG 0.237410 2.2:-34854125 MS.gene67647:CDS
CCCAAGGAAAATCTGAAAAT+TGG 0.266448 2.2:+34856571 None:intergenic
AGGTAAGGTTATGCATTCTA+TGG 0.272924 2.2:-34854501 MS.gene67647:CDS
GAAAATTGGTATGCTTGTTT+TGG 0.274150 2.2:+34856585 None:intergenic
GAAATATCTGCATGTCAAAT+TGG 0.294249 2.2:-34853874 MS.gene67647:CDS
TACCTTTGTTGAGAAATTCT+TGG 0.304695 2.2:+34856323 None:intergenic
GAATCTTATGTAAGGAATAA+TGG 0.308729 2.2:+34856473 None:intergenic
ATATGAAGTCATGGGAATTA+TGG 0.316758 2.2:-34856404 MS.gene67647:CDS
TCTTGAAGAAAACTATGATT+AGG 0.356221 2.2:+34853895 None:intergenic
CTTTGCATTGGGAAATATAG+TGG 0.362241 2.2:-34854575 MS.gene67647:CDS
ACCATTTGCATCTGCGCATT+CGG 0.365561 2.2:+34854382 None:intergenic
GAAACCAGCGCGCAGTTCTA+TGG 0.366149 2.2:+34854233 None:intergenic
ATTCTTGCCACAGGATATAA+AGG 0.366577 2.2:-34853718 MS.gene67647:intron
AATAAGAGAGTTACAGTAGT+AGG 0.371604 2.2:-34854434 MS.gene67647:CDS
AGTGGTAATGGATGTCCTTT+TGG 0.374311 2.2:-34856381 MS.gene67647:CDS
TGCAAATATGCTCTACAGAT+TGG 0.376558 2.2:-34856690 MS.gene67647:CDS
GGGAGGAACTCTGGAATGTT+TGG 0.377591 2.2:+34854548 None:intergenic
TGCCCAAAATGTTGAAATCT+AGG 0.386822 2.2:+34854209 None:intergenic
GTTATTGATATTGATTATGT+TGG 0.393997 2.2:-34856441 MS.gene67647:CDS
TTGGAATTGAATCTTATGTA+AGG 0.401516 2.2:+34856465 None:intergenic
GCCAAGAAGGAAGTGTTCTC+CGG 0.407386 2.2:+34854132 None:intergenic
TTCTATGGATTACTCTTCCT+TGG 0.411045 2.2:-34854486 MS.gene67647:CDS
AAGTTGCCACTGAAGAAGTA+TGG 0.417922 2.2:-34853925 MS.gene67647:CDS
TCTAGAACTTGCTTATTACT+TGG 0.420077 2.2:+34856525 None:intergenic
TTGTTGAAACATATCTGAAA+TGG 0.425780 2.2:-34853948 MS.gene67647:CDS
AGCTTTAGCTTCTGCAAAGA+AGG 0.430511 2.2:-34853787 MS.gene67647:CDS
ATAATCAATATCAATAACTT+TGG 0.441168 2.2:+34856446 None:intergenic
ATGATGGTGTTGGAGGACTA+TGG 0.447006 2.2:-34856641 MS.gene67647:CDS
AGTTTGTTATTCTTTGCATT+GGG 0.451815 2.2:-34854586 MS.gene67647:CDS
GAGTTTGTTATTCTTTGCAT+TGG 0.462117 2.2:-34854587 MS.gene67647:CDS
TTGTGATTTCTTTATCTCGA+TGG 0.463038 2.2:+34853810 None:intergenic
TGTAATATGCCATGTTCCCT+TGG 0.468200 2.2:+34856356 None:intergenic
TGGGAATTATGGAGTGGTAA+TGG 0.469776 2.2:-34856393 MS.gene67647:CDS
CCAGAGTTCCTCCCTGGAAA+AGG 0.479687 2.2:-34854539 MS.gene67647:CDS
GGCCACCTTACTATCACCAT+TGG 0.485362 2.2:-34854104 MS.gene67647:intron
TTGGTCACCTTTATATCCTG+TGG 0.488352 2.2:+34853711 None:intergenic
TCTTACCAATGGTGATAGTA+AGG 0.489855 2.2:+34854099 None:intergenic
AGAGTTGCAATTGTTGGTGC+AGG 0.491948 2.2:-34856732 MS.gene67647:CDS
AACATTCCAGAGTTCCTCCC+TGG 0.496220 2.2:-34854545 MS.gene67647:CDS
TCATCAGCTTCATATACAAC+AGG 0.497177 2.2:+34856660 None:intergenic
AGAGTTACAGTAGTAGGCTC+AGG 0.504894 2.2:-34854428 MS.gene67647:CDS
CATGTTCCCTTGGAGCCAAA+AGG 0.521090 2.2:+34856366 None:intergenic
GTGATAGTAAGGTGGCCAAA+AGG 0.527800 2.2:+34854110 None:intergenic
GATGTAGTTATTCTTGCCAC+AGG 0.531258 2.2:-34853727 MS.gene67647:CDS
CTGATGAAGATATGAAGTCA+TGG 0.535713 2.2:-34856413 MS.gene67647:CDS
TATGAAGCTGATGATGGTGT+TGG 0.543117 2.2:-34856651 MS.gene67647:CDS
AGGTGGCCAAAAGGCCAAGA+AGG 0.545267 2.2:+34854119 None:intergenic
CCAAGAAGGAAGTGTTCTCC+GGG 0.546254 2.2:+34854133 None:intergenic
ATAACTACATCTGTTTCTAA+GGG 0.546830 2.2:+34853739 None:intergenic
GCCGAATGCGCAGATGCAAA+TGG 0.549258 2.2:-34854383 MS.gene67647:intron
GTTGTATATGAAGCTGATGA+TGG 0.564559 2.2:-34856657 MS.gene67647:CDS
GAAGCTGATGATGGTGTTGG+AGG 0.565452 2.2:-34856648 MS.gene67647:CDS
TACCAAGAATTTCTCAACAA+AGG 0.578314 2.2:-34856325 MS.gene67647:intron
TGATGAAGATATGAAGTCAT+GGG 0.593792 2.2:-34856412 MS.gene67647:CDS
GTTCTATGGATAATCGTGCA+AGG 0.619433 2.2:+34854247 None:intergenic
GAGCATATTTGCAAGCAAGT+AGG 0.621227 2.2:+34856700 None:intergenic
TTCTATGGATAATCGTGCAA+GGG 0.621581 2.2:+34854248 None:intergenic
ATTGTTGGTGCAGGAATCAG+TGG 0.624826 2.2:-34856723 MS.gene67647:CDS
TTATCCATAGAACTGCGCGC+TGG 0.626391 2.2:-34854237 MS.gene67647:CDS
ACTAGTCCATACTTCTTCAG+TGG 0.645916 2.2:+34853919 None:intergenic
TACCAATGGTGATAGTAAGG+TGG 0.665277 2.2:+34854102 None:intergenic
AAGTCATGGGAATTATGGAG+TGG 0.671476 2.2:-34856399 MS.gene67647:CDS
GGGAACATGGCATATTACAG+TGG 0.699925 2.2:-34856352 MS.gene67647:CDS
ATGGTGTTGGAGGACTATGG+AGG 0.798552 2.2:-34856638 MS.gene67647:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACCAAAAATTAATATATT+CGG + chr2.2:34855169-34855188 None:intergenic 10.0%
!!! AAAAATCTAAAATAAACATA+AGG - chr2.2:34855565-34855584 MS.gene67647:intron 10.0%
!!! AAAATCTAAAATAAACATAA+GGG - chr2.2:34855566-34855585 MS.gene67647:intron 10.0%
!!! ATATTAAAATTGTGTTTTTA+TGG - chr2.2:34854422-34854441 MS.gene67647:CDS 10.0%
!!! TAAGTAAAAATTTACTTTTT+AGG + chr2.2:34854807-34854826 None:intergenic 10.0%
!!! TTAAAACTTTTAAAACATAA+CGG + chr2.2:34855236-34855255 None:intergenic 10.0%
!!! TTAAAATTTTTAAAACGTAA+GGG + chr2.2:34855265-34855284 None:intergenic 10.0%
!! AAAAAAAAATTCAGGATAAT+TGG + chr2.2:34854476-34854495 None:intergenic 15.0%
!! AAAAAAAATTCAGGATAATT+GGG + chr2.2:34854475-34854494 None:intergenic 15.0%
!! ATAATCAATATCAATAACTT+TGG + chr2.2:34853930-34853949 None:intergenic 15.0%
!! TCTTACAAAAACAAATAAAT+TGG + chr2.2:34856337-34856356 None:intergenic 15.0%
!! TTAAAACCTAAAATACATAA+TGG + chr2.2:34855207-34855226 None:intergenic 15.0%
!! TTGACAAGAAATAATATTTA+GGG - chr2.2:34854504-34854523 MS.gene67647:CDS 15.0%
!! TTTGACAAGAAATAATATTT+AGG - chr2.2:34854503-34854522 MS.gene67647:CDS 15.0%
!!! AGATTTTTTAGATTTTAAGT+TGG + chr2.2:34855553-34855572 None:intergenic 15.0%
!!! ATTGCTTTAAAAAAAAATTC+AGG + chr2.2:34854484-34854503 None:intergenic 15.0%
!!! CTTAAAATTTTTAAAACGTA+AGG + chr2.2:34855266-34855285 None:intergenic 15.0%
!!! TTCATATTTCATTTTCTTTT+TGG - chr2.2:34854115-34854134 MS.gene67647:CDS 15.0%
!!! TTCTTTTACTTTATCAAAAA+AGG + chr2.2:34856539-34856558 None:intergenic 15.0%
!!! TTGAAACTTTTAAAACATAA+GGG - chr2.2:34855497-34855516 MS.gene67647:intron 15.0%
!!! TTTTTTGATAAAGTAAAAGA+AGG - chr2.2:34856538-34856557 MS.gene67647:CDS 15.0%
!! AGAAACATTAGAGATAAATA+AGG - chr2.2:34854201-34854220 MS.gene67647:CDS 20.0%
!! CTTTATGATAAATGTTAACT+CGG + chr2.2:34854542-34854561 None:intergenic 20.0%
!! GAAACATTAGAGATAAATAA+GGG - chr2.2:34854202-34854221 MS.gene67647:CDS 20.0%
!! TTTCATAATTACAAATGTGT+TGG - chr2.2:34854142-34854161 MS.gene67647:CDS 20.0%
!!! ATGTATTTTAGGTTTTAAGT+TGG - chr2.2:34855209-34855228 MS.gene67647:intron 20.0%
!!! ATGTTTTAAAAGTTTCAAGT+TGG + chr2.2:34855495-34855514 None:intergenic 20.0%
!!! CAATTTCACAATTTTAACAA+AGG - chr2.2:34854397-34854416 MS.gene67647:CDS 20.0%
!!! CTCTAGTAAAACTATATTTT+GGG + chr2.2:34854277-34854296 None:intergenic 20.0%
!!! CTTGAAACTTTTAAAACATA+AGG - chr2.2:34855496-34855515 MS.gene67647:intron 20.0%
!!! GTTATTGATATTGATTATGT+TGG - chr2.2:34853932-34853951 MS.gene67647:CDS 20.0%
!!! TAACCAAAAATTGACTTTTT+AGG - chr2.2:34854719-34854738 MS.gene67647:intron 20.0%
!!! TCAATGTAATTTTGGAATAT+TGG + chr2.2:34856712-34856731 None:intergenic 20.0%
!!! TCTCTAGTAAAACTATATTT+TGG + chr2.2:34854278-34854297 None:intergenic 20.0%
!!! TGAATATGCTTTTAAGTTTT+TGG + chr2.2:34856684-34856703 None:intergenic 20.0%
!!! TTGAAACTTTTAAGACATAA+AGG - chr2.2:34855526-34855545 MS.gene67647:intron 20.0%
!!! TTTGATCCATTATGTATTTT+AGG - chr2.2:34855198-34855217 MS.gene67647:intron 20.0%
! AAAAATTAATATATTCGGCG+GGG + chr2.2:34855164-34855183 None:intergenic 25.0%
! AAAATTACATTGACAGTTCA+AGG - chr2.2:34856719-34856738 MS.gene67647:CDS 25.0%
! AAGAGTTTACAAGGATATAT+TGG + chr2.2:34855624-34855643 None:intergenic 25.0%
! ACAAAGTTAAAGAGTTTACA+AGG + chr2.2:34855633-34855652 None:intergenic 25.0%
! ACTTTATCAAAAAAGGAATC+AGG + chr2.2:34856532-34856551 None:intergenic 25.0%
! AGAGATAAATAAGGGAAATA+TGG - chr2.2:34854210-34854229 MS.gene67647:CDS 25.0%
! ATAGTATTGTTGTAATGCAA+AGG + chr2.2:34855726-34855745 None:intergenic 25.0%
! ATTATCAACAAATCACAATG+TGG + chr2.2:34854643-34854662 None:intergenic 25.0%
! CAAAAATTAATATATTCGGC+GGG + chr2.2:34855165-34855184 None:intergenic 25.0%
! CATCTAATAAATGAAGTATG+TGG - chr2.2:34854744-34854763 MS.gene67647:intron 25.0%
! CCAAAAATTAATATATTCGG+CGG + chr2.2:34855166-34855185 None:intergenic 25.0%
! CTTTATCAAAAAAGGAATCA+GGG + chr2.2:34856531-34856550 None:intergenic 25.0%
! GAATCTTATGTAAGGAATAA+TGG + chr2.2:34853903-34853922 None:intergenic 25.0%
! GACTGAATATTTACAAAAAC+AGG - chr2.2:34855752-34855771 MS.gene67647:intron 25.0%
! TAAAGATTAACATGCTATCA+TGG - chr2.2:34855687-34855706 MS.gene67647:intron 25.0%
! TAGTATTGTTGTAATGCAAA+GGG + chr2.2:34855725-34855744 None:intergenic 25.0%
! TATTCAATAAACGATGTATG+TGG + chr2.2:34854782-34854801 None:intergenic 25.0%
! TCTTGAAGAAAACTATGATT+AGG + chr2.2:34856481-34856500 None:intergenic 25.0%
! TTAAACTCAATATCATGTTG+CGG + chr2.2:34855331-34855350 None:intergenic 25.0%
! TTGGAATTGAATCTTATGTA+AGG + chr2.2:34853911-34853930 None:intergenic 25.0%
! TTGTTGAAACATATCTGAAA+TGG - chr2.2:34856425-34856444 MS.gene67647:CDS 25.0%
!! ATGTCTTAAAAGTTTCAAGT+TGG + chr2.2:34855524-34855543 None:intergenic 25.0%
!! GAACCTAAAAAGTCAATTTT+TGG + chr2.2:34854725-34854744 None:intergenic 25.0%
!! TTGAACTGTCAATGTAATTT+TGG + chr2.2:34856720-34856739 None:intergenic 25.0%
!!! AAAATTGGTATGCTTGTTTT+GGG + chr2.2:34853790-34853809 None:intergenic 25.0%
!!! AATAACTACATCTGTTTCTA+AGG + chr2.2:34856638-34856657 None:intergenic 25.0%
!!! AGTTTGTTATTCTTTGCATT+GGG - chr2.2:34855787-34855806 MS.gene67647:intron 25.0%
!!! ATAACTACATCTGTTTCTAA+GGG + chr2.2:34856637-34856656 None:intergenic 25.0%
AATAAGAGAGTTACAGTAGT+AGG - chr2.2:34855939-34855958 MS.gene67647:intron 30.0%
AATGCCAACTCATAAAATTC+AGG + chr2.2:34854957-34854976 None:intergenic 30.0%
ACCAGAGAAAAATCTTATTG+TGG + chr2.2:34854375-34854394 None:intergenic 30.0%
ACTACAATGTATCTTACCAA+TGG + chr2.2:34856288-34856307 None:intergenic 30.0%
ACTTGACCGATTAAAGATTT+GGG + chr2.2:34855429-34855448 None:intergenic 30.0%
AGAACCAAATAAGTCTATCA+AGG + chr2.2:34854859-34854878 None:intergenic 30.0%
AGTACCTTGATAGACTTATT+TGG - chr2.2:34854852-34854871 MS.gene67647:intron 30.0%
AGTGAAATAATTACTCCCTT+CGG - chr2.2:34854687-34854706 MS.gene67647:intron 30.0%
ATATGAAGTCATGGGAATTA+TGG - chr2.2:34853969-34853988 MS.gene67647:CDS 30.0%
GAAAAAAGAGTTGCAATTGT+TGG - chr2.2:34853635-34853654 MS.gene67647:intron 30.0%
GAAATATCTGCATGTCAAAT+TGG - chr2.2:34856499-34856518 MS.gene67647:CDS 30.0%
TACCAAGAATTTCTCAACAA+AGG - chr2.2:34854048-34854067 MS.gene67647:intron 30.0%
TACCTTTGTTGAGAAATTCT+TGG + chr2.2:34854053-34854072 None:intergenic 30.0%
TCATCTGCCACATTAATTTA+TGG - chr2.2:34854892-34854911 MS.gene67647:intron 30.0%
TGATGAAGATATGAAGTCAT+GGG - chr2.2:34853961-34853980 MS.gene67647:CDS 30.0%
TGTCAACCCAAATCTTTAAT+CGG - chr2.2:34855420-34855439 MS.gene67647:intron 30.0%
TTAAGACTTAATTGCGTGTT+TGG + chr2.2:34855593-34855612 None:intergenic 30.0%
TTAATTTATGGTCCAACGAT+TGG - chr2.2:34854904-34854923 MS.gene67647:intron 30.0%
TTCAATCCATTAACTTCTAC+AGG - chr2.2:34856093-34856112 MS.gene67647:intron 30.0%
TTGTGATTTCTTTATCTCGA+TGG + chr2.2:34856566-34856585 None:intergenic 30.0%
! ACCAATTTTCAGATTTTCCT+TGG - chr2.2:34853801-34853820 MS.gene67647:CDS 30.0%
! ACCACAATAAGATTTTTCTC+TGG - chr2.2:34854371-34854390 MS.gene67647:intron 30.0%
! ACTCTAAATTTTCAGAGATG+GGG - chr2.2:34856391-34856410 MS.gene67647:CDS 30.0%
! CAACTCATAAAATTCAGGTT+GGG + chr2.2:34854952-34854971 None:intergenic 30.0%
! CACTCTAAATTTTCAGAGAT+GGG - chr2.2:34856390-34856409 MS.gene67647:CDS 30.0%
! CCAATTTTCAGATTTTCCTT+GGG - chr2.2:34853802-34853821 MS.gene67647:CDS 30.0%
! CCGCCGAATATATTAATTTT+TGG - chr2.2:34855163-34855182 MS.gene67647:intron 30.0%
! CTCCTAGATTTCAACATTTT+GGG - chr2.2:34856162-34856181 MS.gene67647:intron 30.0%
! GAAAATTGGTATGCTTGTTT+TGG + chr2.2:34853791-34853810 None:intergenic 30.0%
! TCTAGAACTTGCTTATTACT+TGG + chr2.2:34853851-34853870 None:intergenic 30.0%
! TCTCCTAGATTTCAACATTT+TGG - chr2.2:34856161-34856180 MS.gene67647:intron 30.0%
! TGTGATACGCTTGATTTTAA+GGG + chr2.2:34855095-34855114 None:intergenic 30.0%
! TTGTGATACGCTTGATTTTA+AGG + chr2.2:34855096-34855115 None:intergenic 30.0%
!! GAGTTTGTTATTCTTTGCAT+TGG - chr2.2:34855786-34855805 MS.gene67647:intron 30.0%
!! TGAATTTTATGAGTTGGCAT+TGG - chr2.2:34854956-34854975 MS.gene67647:intron 30.0%
ACCTTACCTGTAAAAACTTC+TGG + chr2.2:34855861-34855880 None:intergenic 35.0%
AGGTAAGGTTATGCATTCTA+TGG - chr2.2:34855872-34855891 MS.gene67647:intron 35.0%
ATCTTTAATCGGTCAAGTGA+CGG - chr2.2:34855431-34855450 MS.gene67647:intron 35.0%
ATTCCAACGTGAATAACGTA+AGG + chr2.2:34855295-34855314 None:intergenic 35.0%
ATTCTTGCCACAGGATATAA+AGG - chr2.2:34856655-34856674 MS.gene67647:CDS 35.0%
CACTTGACCGATTAAAGATT+TGG + chr2.2:34855430-34855449 None:intergenic 35.0%
CCAACTCATAAAATTCAGGT+TGG + chr2.2:34854953-34854972 None:intergenic 35.0%
CCCAAGGAAAATCTGAAAAT+TGG + chr2.2:34853805-34853824 None:intergenic 35.0%
CCTTACCTGTAAAAACTTCT+GGG + chr2.2:34855860-34855879 None:intergenic 35.0%
CGTTGGACCATAAATTAATG+TGG + chr2.2:34854902-34854921 None:intergenic 35.0%
CTGATGAAGATATGAAGTCA+TGG - chr2.2:34853960-34853979 MS.gene67647:CDS 35.0%
CTTTGCATTGGGAAATATAG+TGG - chr2.2:34855798-34855817 MS.gene67647:intron 35.0%
GTTCAAGGATTTCTCTCTAT+AGG - chr2.2:34856734-34856753 MS.gene67647:CDS 35.0%
GTTGTATATGAAGCTGATGA+TGG - chr2.2:34853716-34853735 MS.gene67647:intron 35.0%
TATAACAGTGAGTTAGTGAC+CGG - chr2.2:34854825-34854844 MS.gene67647:intron 35.0%
TCATCAGCTTCATATACAAC+AGG + chr2.2:34853716-34853735 None:intergenic 35.0%
TCTTACCAATGGTGATAGTA+AGG + chr2.2:34856277-34856296 None:intergenic 35.0%
TGCAAATATGCTCTACAGAT+TGG - chr2.2:34853683-34853702 MS.gene67647:intron 35.0%
TGCCCAAAATGTTGAAATCT+AGG + chr2.2:34856167-34856186 None:intergenic 35.0%
TGGTTATAATAGTGACCGAA+GGG + chr2.2:34854705-34854724 None:intergenic 35.0%
TTCCAACGTGAATAACGTAA+GGG + chr2.2:34855294-34855313 None:intergenic 35.0%
TTCTATGGATAATCGTGCAA+GGG + chr2.2:34856128-34856147 None:intergenic 35.0%
TTGGTTATAATAGTGACCGA+AGG + chr2.2:34854706-34854725 None:intergenic 35.0%
! AACAGCATTTTCTTTGTCCA+AGG + chr2.2:34855907-34855926 None:intergenic 35.0%
! CCAACCTGAATTTTATGAGT+TGG - chr2.2:34854950-34854969 MS.gene67647:intron 35.0%
! CCACTCATAAAATTCAGGTT+GGG + chr2.2:34854983-34855002 None:intergenic 35.0%
! GCACTCTAAATTTTCAGAGA+TGG - chr2.2:34856389-34856408 MS.gene67647:CDS 35.0%
! TCTTTTACAGAAGAATCCCA+AGG + chr2.2:34853821-34853840 None:intergenic 35.0%
! TTCTATGGATTACTCTTCCT+TGG - chr2.2:34855887-34855906 MS.gene67647:intron 35.0%
!! AAAGATTTGGGTTGACAATG+TGG + chr2.2:34855417-34855436 None:intergenic 35.0%
!! AACATTTTGGGCATTAGTCT+CGG - chr2.2:34856174-34856193 MS.gene67647:intron 35.0%
!! AAGATTTGGGTTGACAATGT+GGG + chr2.2:34855416-34855435 None:intergenic 35.0%
AAGTCATGGGAATTATGGAG+TGG - chr2.2:34853974-34853993 MS.gene67647:intron 40.0%
AAGTTGCCACTGAAGAAGTA+TGG - chr2.2:34856448-34856467 MS.gene67647:CDS 40.0%
ACTAGTCCATACTTCTTCAG+TGG + chr2.2:34856457-34856476 None:intergenic 40.0%
ATCCCTTACGTTATTCACGT+TGG - chr2.2:34855289-34855308 MS.gene67647:intron 40.0%
CTCAATATCATGTTGCGGAA+TGG + chr2.2:34855326-34855345 None:intergenic 40.0%
GACAGCCACTCATAAAATTC+AGG + chr2.2:34854988-34855007 None:intergenic 40.0%
GAGCATATTTGCAAGCAAGT+AGG + chr2.2:34853676-34853695 None:intergenic 40.0%
GATGTAGTTATTCTTGCCAC+AGG - chr2.2:34856646-34856665 MS.gene67647:CDS 40.0%
GCCACTCATAAAATTCAGGT+TGG + chr2.2:34854984-34855003 None:intergenic 40.0%
GTGACTCCTGTAGAAGTTAA+TGG + chr2.2:34856102-34856121 None:intergenic 40.0%
GTTCTATGGATAATCGTGCA+AGG + chr2.2:34856129-34856148 None:intergenic 40.0%
TACCAATGGTGATAGTAAGG+TGG + chr2.2:34856274-34856293 None:intergenic 40.0%
TGGGAATTATGGAGTGGTAA+TGG - chr2.2:34853980-34853999 MS.gene67647:intron 40.0%
TGTAATATGCCATGTTCCCT+TGG + chr2.2:34854020-34854039 None:intergenic 40.0%
TTGGTCACCTTTATATCCTG+TGG + chr2.2:34856665-34856684 None:intergenic 40.0%
! AAAGGCCCAGAAGTTTTTAC+AGG - chr2.2:34855852-34855871 MS.gene67647:intron 40.0%
! AGCTTTAGCTTCTGCAAAGA+AGG - chr2.2:34856586-34856605 MS.gene67647:CDS 40.0%
! CCCAACCTGAATTTTATGAG+TGG - chr2.2:34854949-34854968 MS.gene67647:intron 40.0%
! CCCAGAAGTTTTTACAGGTA+AGG - chr2.2:34855857-34855876 MS.gene67647:intron 40.0%
!! TATGAAGCTGATGATGGTGT+TGG - chr2.2:34853722-34853741 MS.gene67647:CDS 40.0%
!! TTTGCTCAGACTAGTTTGCT+CGG - chr2.2:34855013-34855032 MS.gene67647:intron 40.0%
!!! AGTGGTAATGGATGTCCTTT+TGG - chr2.2:34853992-34854011 MS.gene67647:intron 40.0%
!!! GAACTTTTAGTTCACAAGCC+CGG - chr2.2:34856222-34856241 MS.gene67647:intron 40.0%
!!! GGTATGCTTGTTTTGGGTTT+TGG + chr2.2:34853784-34853803 None:intergenic 40.0%
ACCATTTGCATCTGCGCATT+CGG + chr2.2:34855994-34856013 None:intergenic 45.0%
ACGATTGGTGTGGTGCATTA+TGG - chr2.2:34854919-34854938 MS.gene67647:intron 45.0%
AGAGTTACAGTAGTAGGCTC+AGG - chr2.2:34855945-34855964 MS.gene67647:intron 45.0%
AGAGTTGCAATTGTTGGTGC+AGG - chr2.2:34853641-34853660 MS.gene67647:intron 45.0%
ATTGTTGGTGCAGGAATCAG+TGG - chr2.2:34853650-34853669 MS.gene67647:intron 45.0%
GGGAACATGGCATATTACAG+TGG - chr2.2:34854021-34854040 MS.gene67647:intron 45.0%
GTGATAGTAAGGTGGCCAAA+AGG + chr2.2:34856266-34856285 None:intergenic 45.0%
TAATGCACCACACCAATCGT+TGG + chr2.2:34854919-34854938 None:intergenic 45.0%
TTATGGTCCAACGATTGGTG+TGG - chr2.2:34854909-34854928 MS.gene67647:intron 45.0%
! AAAACTTCTGGGCCTTTTCC+AGG + chr2.2:34855849-34855868 None:intergenic 45.0%
! AAACTTCTGGGCCTTTTCCA+GGG + chr2.2:34855848-34855867 None:intergenic 45.0%
! ATGATGGTGTTGGAGGACTA+TGG - chr2.2:34853732-34853751 MS.gene67647:CDS 45.0%
!! ACACTTCCTTCTTGGCCTTT+TGG - chr2.2:34856248-34856267 MS.gene67647:intron 45.0%
!! ATTTGGGTTGACAATGTGGG+TGG + chr2.2:34855413-34855432 None:intergenic 45.0%
AACAGTGAGTTAGTGACCGG+AGG - chr2.2:34854828-34854847 MS.gene67647:intron 50.0%
AACATTCCAGAGTTCCTCCC+TGG - chr2.2:34855828-34855847 MS.gene67647:intron 50.0%
ACAGTGAGTTAGTGACCGGA+GGG - chr2.2:34854829-34854848 MS.gene67647:intron 50.0%
CATGTTCCCTTGGAGCCAAA+AGG + chr2.2:34854010-34854029 None:intergenic 50.0%
GGCCACCTTACTATCACCAT+TGG - chr2.2:34856269-34856288 MS.gene67647:intron 50.0%
GGTGTGGTGCATTATGGCAT+TGG - chr2.2:34854925-34854944 MS.gene67647:intron 50.0%
TTATCCATAGAACTGCGCGC+TGG - chr2.2:34856136-34856155 MS.gene67647:intron 50.0%
! ATGGTGTTGGAGGACTATGG+AGG - chr2.2:34853735-34853754 MS.gene67647:CDS 50.0%
! GTCTATCAAGGTACTCCCTC+CGG + chr2.2:34854847-34854866 None:intergenic 50.0%
!! CCAAGAAGGAAGTGTTCTCC+GGG + chr2.2:34856243-34856262 None:intergenic 50.0%
!! GAAGCTGATGATGGTGTTGG+AGG - chr2.2:34853725-34853744 MS.gene67647:CDS 50.0%
!! GCCAAGAAGGAAGTGTTCTC+CGG + chr2.2:34856244-34856263 None:intergenic 50.0%
!! GGGAGGAACTCTGGAATGTT+TGG + chr2.2:34855828-34855847 None:intergenic 50.0%
!!! CTTTTGGCTCCAAGGGAACA+TGG - chr2.2:34854008-34854027 MS.gene67647:intron 50.0%
!!! GGATGTCCTTTTGGCTCCAA+GGG - chr2.2:34854001-34854020 MS.gene67647:intron 50.0%
!!! TGGATGTCCTTTTGGCTCCA+AGG - chr2.2:34854000-34854019 MS.gene67647:intron 50.0%
AGGTGGCCAAAAGGCCAAGA+AGG + chr2.2:34856257-34856276 None:intergenic 55.0%
CCAGAGTTCCTCCCTGGAAA+AGG - chr2.2:34855834-34855853 MS.gene67647:intron 55.0%
CCCGGAGAACACTTCCTTCT+TGG - chr2.2:34856240-34856259 MS.gene67647:intron 55.0%
GAAACCAGCGCGCAGTTCTA+TGG + chr2.2:34856143-34856162 None:intergenic 55.0%
GCCGAATGCGCAGATGCAAA+TGG - chr2.2:34855990-34856009 MS.gene67647:intron 55.0%
! CCTTTTCCAGGGAGGAACTC+TGG + chr2.2:34855837-34855856 None:intergenic 55.0%
! TGGGTTGACAATGTGGGTGG+TGG + chr2.2:34855410-34855429 None:intergenic 55.0%
! CTTCTGGGCCTTTTCCAGGG+AGG + chr2.2:34855845-34855864 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 34853632 34856763 34853632 ID=MS.gene67647
chr2.2 mRNA 34853632 34856763 34853632 ID=MS.gene67647.t1;Parent=MS.gene67647
chr2.2 exon 34856326 34856763 34856326 ID=MS.gene67647.t1.exon1;Parent=MS.gene67647.t1
chr2.2 CDS 34856326 34856763 34856326 ID=cds.MS.gene67647.t1;Parent=MS.gene67647.t1
chr2.2 exon 34854384 34854621 34854384 ID=MS.gene67647.t1.exon2;Parent=MS.gene67647.t1
chr2.2 CDS 34854384 34854621 34854384 ID=cds.MS.gene67647.t1;Parent=MS.gene67647.t1
chr2.2 exon 34854105 34854280 34854105 ID=MS.gene67647.t1.exon3;Parent=MS.gene67647.t1
chr2.2 CDS 34854105 34854280 34854105 ID=cds.MS.gene67647.t1;Parent=MS.gene67647.t1
chr2.2 exon 34853719 34853989 34853719 ID=MS.gene67647.t1.exon4;Parent=MS.gene67647.t1
chr2.2 CDS 34853719 34853989 34853719 ID=cds.MS.gene67647.t1;Parent=MS.gene67647.t1
chr2.2 exon 34853632 34853639 34853632 ID=MS.gene67647.t1.exon5;Parent=MS.gene67647.t1
chr2.2 CDS 34853632 34853639 34853632 ID=cds.MS.gene67647.t1;Parent=MS.gene67647.t1
Gene Sequence

>MS.gene67647

ATGGAAAAAAGAGTTGCAATTGTTGGTGCAGGAATCAGTGGCCTACTTGCTTGCAAATATGCTCTACAGATTGGTTTTAATCCTGTTGTATATGAAGCTGATGATGGTGTTGGAGGACTATGGAGGCATACTATTGAATCTACAAAACTCCAAAACCCAAAACAAGCATACCAATTTTCAGATTTTCCTTGGGATTCTTCTGTAAAAGAAGATTGTCCAAGTAATAAGCAAGTTCTAGATTATGTTGAATCATATGCTCAACATTTTTCCATTATTCCTTACATAAGATTCAATTCCAAAGTTATTGATATTGATTATGTTGGAGAGTCTGATGAAGATATGAAGTCATGGGAATTATGGAGTGGTAATGGATGTCCTTTTGGCTCCAAGGGAACATGGCATATTACAGTGGAAGATACCAAGAATTTCTCAACAAAGGTATGTTTTATATAGATTGTAATTTTGTGCAAAGTTTGCATAACTTTCATATTTCATTTTCTTTTTGGTTCGTTTCATAATTACAAATGTGTTGGAAAAATAAACATAGAATTATCAAAATATTATAAACAAGAAACATTAGAGATAAATAAGGGAAATATGGTTTGAATAAAAAAAAAAAGTGAAATTAATGTGTTAGTCTTTCCCAAAATATAGTTTTACTAGAGATTTCCCAAAATATAGTTTTACTAGAGATCTGAAAGATTAATTTATGCAATTATCTAATTTTTTATTTTTAGTTACCACAATAAGATTTTTCTCTGGTAACAATTTCACAATTTTAACAAAGGTTATATTAAAATTGTGTTTTTATGGAAGAAATTTGTTGTCAACAAAAGTTAACCCAATTATCCTGAATTTTTTTTTAAAGCAATTTGACAAGAAATAATATTTAGGGATGTCAGAGCAACCGAGTTAACATTTATCATAAAGAAATTACGAAGAAAAACACTAACTTAACAATTAAAAAACTAACTTTTGTGATTAAAAAAAACATAATTACTTCAAAAGCCACATTGTGATTTGTTGATAATATAATTTTTTTTAATGTAAATGTCAGTGAAATAATTACTCCCTTCGGTCACTATTATAACCAAAAATTGACTTTTTAGGTTCATCTAATAAATGAAGTATGTGGTCTATATATAGACCACATACATCGTTTATTGAATAAACCTAAAAAGTAAATTTTTACTTATAACAGTGAGTTAGTGACCGGAGGGAGTACCTTGATAGACTTATTTGGTTCTGCGAATAATGATCTCATCTGCCACATTAATTTATGGTCCAACGATTGGTGTGGTGCATTATGGCATTGGACCCAACCTGAATTTTATGAGTTGGCATTGGACCCAACCTGAATTTTATGAGTGGCTGTCAAAAGTTTGCTCAGACTAGTTTGCTCGGACTTTTGAAGAGCTGAAATCAACTTGACATTCGTACATGTGAATCTATTATGTGTACCCTTAAAATCAAGCGTATCACAATTTATTAAGCGTGTAAACTCAACTTCTAAAGATGTTGTAAAGTATCCCCGCCGAATATATTAATTTTTGGTTTAATTGCATATTTGATCCATTATGTATTTTAGGTTTTAAGTTGGTCCGTTATGTTTTAAAAGTTTTAAGTTAGTCCCTTACGTTTTAAAAATTTTAAGTTGATCCCTTACGTTATTCACGTTGGAATAAGTTAGTCCATTCCGCAACATGATATTGAGTTTAATAAAATAGAAAAATTAAAAAAATATTTTAAAACTGATTTTTTAAATAAGTTAAATTCCACCACCCACATTGTCAACCCAAATCTTTAATCGGTCAAGTGACGGAAGTGACTAACTTATTCAAATGTGAGTAAAGTTTTGTACCAACTTGAAACTTTTAAAACATAAGGGACCAACTTGAAACTTTTAAGACATAAAGGACCAACTTAAAATCTAAAAAATCTAAAATAAACATAAGGGACCAAACACGCAATTAAGTCTTAATTTTTCAACCAATATATCCTTGTAAACTCTTTAACTTTGTCACTAAAACATAAATTGATCTAGCAGCAAGTTAATAAAGATTAACATGCTATCATGGTGTAAAATGTTTCCCTTTGCATTACAACAATACTATAGACTAGACTGAATATTTACAAAAACAGGTGCATAAAGCAGAGTTTGTTATTCTTTGCATTGGGAAATATAGTGGTTTTCCAAACATTCCAGAGTTCCTCCCTGGAAAAGGCCCAGAAGTTTTTACAGGTAAGGTTATGCATTCTATGGATTACTCTTCCTTGGACAAAGAAAATGCTGTTGAACTGATAAAAAATAAGAGAGTTACAGTAGTAGGCTCAGGAAAATCTGCTCTTGATATAGCTGCCGAATGCGCAGATGCAAATGGTGAGAATATATTTGCTCAAATATTTACAATATTCAAATTATTTTGTGTTACGACACTTCTTGATAGCACTTAATTTGAATTTCAATCCATTAACTTCTACAGGAGTCACTCACCCTTGCACGATTATCCATAGAACTGCGCGCTGGTTTCTCCTAGATTTCAACATTTTGGGCATTAGTCTCGGATACTTCTATTTCAATCGTTTCGCAGAACTTTTAGTTCACAAGCCCGGAGAACACTTCCTTCTTGGCCTTTTGGCCACCTTACTATCACCATTGGTAAGATACATTGTAGTATTATATTATCATATACGTTTCATAACCAATTTATTTGTTTTTGTAAGAGTGAACATTGTGTTCATAATTTATACATATTTGCACTCTAAATTTTCAGAGATGGGGAATTTCTAAACTTGTTGAAACATATCTGAAATGGAAGTTGCCACTGAAGAAGTATGGACTAGTACCTAATCATAGTTTTCTTCAAGAAATATCTGCATGTCAAATTGGAATTCTCCCTGATTCCTTTTTTGATAAAGTAAAAGAAGGATCCATCGAGATAAAGAAATCACAAAGCTTTAGCTTCTGCAAAGAAGGTTTGATCATCAATGAAGAAGCTAAGCCCTTAGAAACAGATGTAGTTATTCTTGCCACAGGATATAAAGGTGACCAAAAACTTAAAAGCATATTCAAATCTCCAATATTCCAAAATTACATTGACAGTTCAAGGATTTCTCTCTATAGGTCAGTAA
Protein sequence

>MS.gene67647.t1

MEKRVAIVGAGISGLLACKYALQIGFNPVVYEADDGVGGLWRHTIESTKLQNPKQAYQFSDFPWDSSVKEDCPSNKQVLDYVESYAQHFSIIPYIRFNSKVIDIDYVGESDEDMKSWELWSGNGCPFGSKGTWHITVEDTKNFSTKVHKAEFVILCIGKYSGFPNIPEFLPGKGPEVFTGKVMHSMDYSSLDKENAVELIKNKRVTVVGSGKSALDIAAECADANGVTHPCTIIHRTARWFLLDFNILGISLGYFYFNRFAELLVHKPGEHFLLGLLATLLSPLRWGISKLVETYLKWKLPLKKYGLVPNHSFLQEISACQIGILPDSFFDKVKEGSIEIKKSQSFSFCKEGLIINEEAKPLETDVVILATGYKGQ