Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05858.t1 | XP_003630873.2 | 95.8 | 1042 | 43 | 1 | 1 | 1041 | 63 | 1104 | 0.00E+00 | 2015.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05858.t1 | Q8LPQ5 | 65.6 | 1048 | 340 | 9 | 1 | 1041 | 77 | 1111 | 0.0e+00 | 1366.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05858.t1 | G7L9R6 | 95.8 | 1042 | 43 | 1 | 1 | 1041 | 63 | 1104 | 0.0e+00 | 2015.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049369 | MS.gene05858 | 0.805228 | 1.48E-49 | -1.69E-46 |
| MS.gene057449 | MS.gene05858 | 0.80398 | 2.69E-49 | -1.69E-46 |
| MS.gene057537 | MS.gene05858 | 0.821249 | 4.46E-53 | -1.69E-46 |
| MS.gene058075 | MS.gene05858 | 0.802579 | 5.25E-49 | -1.69E-46 |
| MS.gene05856 | MS.gene05858 | 0.854854 | 9.33E-62 | -1.69E-46 |
| MS.gene05858 | MS.gene061659 | 0.802122 | 6.52E-49 | -1.69E-46 |
| MS.gene05858 | MS.gene067322 | 0.817823 | 2.70E-52 | -1.69E-46 |
| MS.gene05858 | MS.gene070491 | 0.839789 | 1.28E-57 | -1.69E-46 |
| MS.gene05858 | MS.gene072035 | 0.804439 | 2.16E-49 | -1.69E-46 |
| MS.gene05858 | MS.gene073007 | 0.809997 | 1.43E-50 | -1.69E-46 |
| MS.gene05858 | MS.gene23368 | 0.815316 | 9.84E-52 | -1.69E-46 |
| MS.gene05858 | MS.gene29844 | 0.805043 | 1.61E-49 | -1.69E-46 |
| MS.gene05858 | MS.gene31046 | 0.814252 | 1.69E-51 | -1.69E-46 |
| MS.gene05858 | MS.gene31206 | 0.815967 | 7.05E-52 | -1.69E-46 |
| MS.gene05858 | MS.gene31207 | 0.825324 | 4.99E-54 | -1.69E-46 |
| MS.gene05858 | MS.gene31579 | 0.823997 | 1.02E-53 | -1.69E-46 |
| MS.gene05858 | MS.gene34376 | 0.801171 | 1.02E-48 | -1.69E-46 |
| MS.gene05858 | MS.gene37002 | 0.805904 | 1.07E-49 | -1.69E-46 |
| MS.gene05858 | MS.gene37910 | 0.841838 | 3.73E-58 | -1.69E-46 |
| MS.gene05858 | MS.gene39231 | 0.861881 | 7.51E-64 | -1.69E-46 |
| MS.gene05858 | MS.gene39360 | 0.80372 | 3.05E-49 | -1.69E-46 |
| MS.gene05858 | MS.gene40499 | 0.811718 | 6.09E-51 | -1.69E-46 |
| MS.gene05858 | MS.gene43710 | 0.844294 | 8.26E-59 | -1.69E-46 |
| MS.gene05858 | MS.gene44820 | 0.826136 | 3.20E-54 | -1.69E-46 |
| MS.gene05858 | MS.gene45237 | 0.81716 | 3.81E-52 | -1.69E-46 |
| MS.gene05858 | MS.gene46503 | 0.86407 | 1.58E-64 | -1.69E-46 |
| MS.gene05858 | MS.gene46957 | 0.801579 | 8.42E-49 | -1.69E-46 |
| MS.gene05858 | MS.gene51807 | 0.817731 | 2.83E-52 | -1.69E-46 |
| MS.gene05858 | MS.gene57035 | 0.862125 | 6.32E-64 | -1.69E-46 |
| MS.gene05858 | MS.gene62624 | 0.812196 | 4.79E-51 | -1.69E-46 |
| MS.gene05858 | MS.gene67647 | 0.803264 | 3.79E-49 | -1.69E-46 |
| MS.gene05858 | MS.gene68967 | 0.801404 | 9.15E-49 | -1.69E-46 |
| MS.gene05858 | MS.gene68968 | 0.800175 | 1.63E-48 | -1.69E-46 |
| MS.gene05858 | MS.gene77339 | 0.80622 | 9.14E-50 | -1.69E-46 |
| MS.gene05858 | MS.gene77419 | 0.800366 | 1.49E-48 | -1.69E-46 |
| MS.gene05858 | MS.gene78431 | 0.803615 | 3.20E-49 | -1.69E-46 |
| MS.gene05858 | MS.gene81602 | 0.845726 | 3.39E-59 | -1.69E-46 |
| MS.gene05858 | MS.gene84200 | 0.807686 | 4.48E-50 | -1.69E-46 |
| MS.gene05858 | MS.gene84466 | 0.821187 | 4.61E-53 | -1.69E-46 |
| MS.gene05858 | MS.gene85662 | 0.80658 | 7.68E-50 | -1.69E-46 |
| MS.gene05858 | MS.gene88655 | 0.800442 | 1.44E-48 | -1.69E-46 |
| MS.gene05858 | MS.gene90567 | 0.82147 | 3.97E-53 | -1.69E-46 |
| MS.gene05858 | MS.gene95806 | 0.804864 | 1.76E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05858.t1 | MTR_8g104410 | 97.217 | 1042 | 28 | 1 | 1 | 1041 | 63 | 1104 | 0.0 | 2101 |
| MS.gene05858.t1 | MTR_8g104410 | 26.761 | 213 | 123 | 4 | 587 | 799 | 38 | 217 | 1.72e-20 | 98.6 |
| MS.gene05858.t1 | MTR_8g104410 | 97.120 | 868 | 24 | 1 | 175 | 1041 | 1 | 868 | 0.0 | 1749 |
| MS.gene05858.t1 | MTR_8g104410 | 24.286 | 210 | 126 | 3 | 11 | 187 | 449 | 658 | 7.88e-16 | 83.2 |
| MS.gene05858.t1 | MTR_8g104410 | 31.373 | 153 | 94 | 2 | 5 | 151 | 97 | 244 | 1.99e-15 | 82.0 |
| MS.gene05858.t1 | MTR_6g083900 | 79.406 | 1044 | 195 | 7 | 1 | 1041 | 68 | 1094 | 0.0 | 1698 |
| MS.gene05858.t1 | MTR_4g107440 | 56.995 | 193 | 81 | 2 | 854 | 1045 | 954 | 1145 | 3.76e-67 | 247 |
| MS.gene05858.t1 | MTR_4g107440 | 34.452 | 447 | 266 | 7 | 8 | 441 | 74 | 506 | 2.16e-63 | 235 |
| MS.gene05858.t1 | MTR_4g107440 | 42.085 | 259 | 136 | 3 | 560 | 818 | 591 | 835 | 4.19e-52 | 200 |
| MS.gene05858.t1 | MTR_4g107440 | 24.797 | 246 | 162 | 6 | 225 | 449 | 603 | 846 | 7.81e-14 | 77.0 |
| MS.gene05858.t1 | MTR_4g107440 | 27.136 | 199 | 121 | 6 | 9 | 187 | 653 | 847 | 1.24e-13 | 76.3 |
| MS.gene05858.t1 | MTR_4g107440 | 26.415 | 212 | 120 | 6 | 589 | 794 | 38 | 219 | 8.51e-12 | 70.1 |
| MS.gene05858.t1 | MTR_2g020040 | 33.846 | 130 | 71 | 4 | 928 | 1047 | 10 | 134 | 5.10e-20 | 91.7 |
| MS.gene05858.t1 | MTR_1g047740 | 33.566 | 143 | 75 | 5 | 909 | 1040 | 33 | 166 | 8.44e-20 | 91.7 |
| MS.gene05858.t1 | MTR_3g108240 | 28.994 | 169 | 95 | 5 | 890 | 1047 | 27 | 181 | 3.80e-18 | 87.0 |
| MS.gene05858.t1 | MTR_7g102990 | 33.333 | 144 | 78 | 5 | 915 | 1047 | 5 | 141 | 6.40e-17 | 81.3 |
| MS.gene05858.t1 | MTR_7g102990 | 35.036 | 137 | 71 | 5 | 915 | 1040 | 5 | 134 | 9.26e-17 | 82.8 |
| MS.gene05858.t1 | MTR_4g057310 | 34.127 | 126 | 65 | 5 | 934 | 1047 | 19 | 138 | 1.64e-15 | 77.4 |
| MS.gene05858.t1 | MTR_4g057310 | 34.127 | 126 | 65 | 5 | 934 | 1047 | 16 | 135 | 1.69e-15 | 78.6 |
| MS.gene05858.t1 | MTR_3g108240 | 27.811 | 169 | 95 | 5 | 890 | 1047 | 27 | 179 | 2.08e-15 | 79.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05858.t1 | AT3G18290 | 65.972 | 1055 | 327 | 12 | 1 | 1042 | 77 | 1112 | 0.0 | 1397 |
| MS.gene05858.t1 | AT3G18290 | 25.283 | 265 | 159 | 5 | 589 | 853 | 54 | 279 | 9.68e-19 | 92.8 |
| MS.gene05858.t1 | AT1G74770 | 31.373 | 816 | 488 | 18 | 17 | 818 | 75 | 832 | 2.07e-112 | 377 |
| MS.gene05858.t1 | AT1G74770 | 53.241 | 216 | 92 | 3 | 838 | 1045 | 919 | 1133 | 1.42e-65 | 242 |
| MS.gene05858.t1 | AT1G74770 | 25.498 | 251 | 147 | 7 | 563 | 807 | 8 | 224 | 2.72e-13 | 75.1 |
| MS.gene05858.t1 | AT1G74770 | 31.450 | 814 | 486 | 18 | 17 | 816 | 75 | 830 | 2.53e-112 | 377 |
| MS.gene05858.t1 | AT1G74770 | 53.241 | 216 | 92 | 3 | 838 | 1045 | 919 | 1133 | 2.47e-65 | 241 |
| MS.gene05858.t1 | AT1G74770 | 25.498 | 251 | 147 | 7 | 563 | 807 | 8 | 224 | 2.52e-13 | 75.1 |
| MS.gene05858.t1 | AT1G18910 | 49.770 | 217 | 97 | 2 | 840 | 1045 | 913 | 1128 | 1.81e-63 | 236 |
| MS.gene05858.t1 | AT1G18910 | 31.891 | 439 | 273 | 8 | 14 | 441 | 80 | 503 | 5.85e-57 | 215 |
| MS.gene05858.t1 | AT1G18910 | 42.041 | 245 | 120 | 4 | 588 | 832 | 610 | 832 | 1.31e-48 | 189 |
| MS.gene05858.t1 | AT1G18910 | 26.496 | 234 | 137 | 5 | 586 | 814 | 41 | 244 | 1.08e-17 | 89.4 |
| MS.gene05858.t1 | AT1G18910 | 30.137 | 146 | 89 | 3 | 17 | 149 | 649 | 794 | 4.21e-12 | 71.2 |
| MS.gene05858.t1 | AT3G62970 | 38.519 | 135 | 66 | 5 | 924 | 1047 | 23 | 151 | 2.84e-23 | 100 |
| MS.gene05858.t1 | AT3G62970 | 38.519 | 135 | 66 | 5 | 924 | 1047 | 23 | 151 | 4.26e-23 | 99.8 |
| MS.gene05858.t1 | AT3G62970 | 38.519 | 135 | 66 | 5 | 924 | 1047 | 23 | 151 | 4.79e-23 | 100 |
| MS.gene05858.t1 | AT5G18650 | 36.719 | 128 | 66 | 5 | 930 | 1047 | 12 | 134 | 2.61e-19 | 89.7 |
| MS.gene05858.t1 | AT5G25560 | 32.824 | 131 | 72 | 4 | 928 | 1047 | 55 | 180 | 5.69e-18 | 86.7 |
| MS.gene05858.t1 | AT5G25560 | 32.824 | 131 | 72 | 4 | 928 | 1047 | 55 | 180 | 5.69e-18 | 86.7 |
| MS.gene05858.t1 | AT5G25560 | 32.824 | 131 | 72 | 4 | 928 | 1047 | 55 | 180 | 5.69e-18 | 86.7 |
| MS.gene05858.t1 | AT5G25560 | 32.824 | 131 | 72 | 4 | 928 | 1047 | 55 | 180 | 5.69e-18 | 86.7 |
| MS.gene05858.t1 | AT5G22920 | 34.884 | 129 | 68 | 4 | 930 | 1047 | 21 | 144 | 6.73e-17 | 82.0 |
| MS.gene05858.t1 | AT5G22920 | 34.884 | 129 | 68 | 4 | 930 | 1047 | 21 | 144 | 1.06e-16 | 82.4 |
| MS.gene05858.t1 | AT5G25560 | 30.496 | 141 | 72 | 5 | 928 | 1047 | 55 | 190 | 9.48e-16 | 80.1 |
| MS.gene05858.t1 | AT5G25560 | 31.667 | 120 | 66 | 4 | 939 | 1047 | 61 | 175 | 7.31e-14 | 74.3 |
Find 0 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57356 | gene | 52 | 10399 | 52 | ID=MS.gene05858 |
| 57356 | mRNA | 52 | 10399 | 52 | ID=MS.gene05858.t1;Parent=MS.gene05858 |
| 57356 | exon | 10298 | 10399 | 10298 | ID=MS.gene05858.t1.exon1;Parent=MS.gene05858.t1 |
| 57356 | CDS | 10298 | 10399 | 10298 | ID=cds.MS.gene05858.t1;Parent=MS.gene05858.t1 |
| 57356 | exon | 9028 | 9237 | 9028 | ID=MS.gene05858.t1.exon2;Parent=MS.gene05858.t1 |
| 57356 | CDS | 9028 | 9237 | 9028 | ID=cds.MS.gene05858.t1;Parent=MS.gene05858.t1 |
| 57356 | exon | 6662 | 6856 | 6662 | ID=MS.gene05858.t1.exon3;Parent=MS.gene05858.t1 |
| 57356 | CDS | 6662 | 6856 | 6662 | ID=cds.MS.gene05858.t1;Parent=MS.gene05858.t1 |
| 57356 | exon | 5462 | 5835 | 5462 | ID=MS.gene05858.t1.exon4;Parent=MS.gene05858.t1 |
| 57356 | CDS | 5462 | 5835 | 5462 | ID=cds.MS.gene05858.t1;Parent=MS.gene05858.t1 |
| 57356 | exon | 5094 | 5322 | 5094 | ID=MS.gene05858.t1.exon5;Parent=MS.gene05858.t1 |
| 57356 | CDS | 5094 | 5322 | 5094 | ID=cds.MS.gene05858.t1;Parent=MS.gene05858.t1 |
| 57356 | exon | 4643 | 4811 | 4643 | ID=MS.gene05858.t1.exon6;Parent=MS.gene05858.t1 |
| 57356 | CDS | 4643 | 4811 | 4643 | ID=cds.MS.gene05858.t1;Parent=MS.gene05858.t1 |
| 57356 | exon | 3306 | 4541 | 3306 | ID=MS.gene05858.t1.exon7;Parent=MS.gene05858.t1 |
| 57356 | CDS | 3306 | 4541 | 3306 | ID=cds.MS.gene05858.t1;Parent=MS.gene05858.t1 |
| 57356 | exon | 3057 | 3228 | 3057 | ID=MS.gene05858.t1.exon8;Parent=MS.gene05858.t1 |
| 57356 | CDS | 3057 | 3228 | 3057 | ID=cds.MS.gene05858.t1;Parent=MS.gene05858.t1 |
| 57356 | exon | 2707 | 2928 | 2707 | ID=MS.gene05858.t1.exon9;Parent=MS.gene05858.t1 |
| 57356 | CDS | 2707 | 2928 | 2707 | ID=cds.MS.gene05858.t1;Parent=MS.gene05858.t1 |
| 57356 | exon | 2406 | 2543 | 2406 | ID=MS.gene05858.t1.exon10;Parent=MS.gene05858.t1 |
| 57356 | CDS | 2406 | 2543 | 2406 | ID=cds.MS.gene05858.t1;Parent=MS.gene05858.t1 |
| 57356 | exon | 52 | 151 | 52 | ID=MS.gene05858.t1.exon11;Parent=MS.gene05858.t1 |
| 57356 | CDS | 52 | 151 | 52 | ID=cds.MS.gene05858.t1;Parent=MS.gene05858.t1 |
| Gene Sequence |
| Protein sequence |
