AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene67862

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67862.t1 MTR_2g032710 96.930 456 8 1 1 456 14 463 0.0 912
MS.gene67862.t1 MTR_4g094715 73.961 457 108 2 1 456 15 461 0.0 701
MS.gene67862.t1 MTR_1g086390 63.077 390 142 1 59 446 80 469 0.0 547
MS.gene67862.t1 MTR_7g110050 60.000 425 160 4 32 454 46 462 0.0 538
MS.gene67862.t1 MTR_5g034090 46.608 457 204 9 18 444 77 523 2.84e-132 392
MS.gene67862.t1 MTR_8g065010 47.552 429 189 7 41 443 91 509 4.51e-130 386
MS.gene67862.t1 MTR_6g005630 46.512 387 189 6 60 445 45 414 4.25e-119 355
MS.gene67862.t1 MTR_5g033680 41.584 404 217 8 43 441 50 439 1.17e-106 325
MS.gene67862.t1 MTR_6g005630 43.411 387 178 6 60 445 45 391 3.67e-105 318
MS.gene67862.t1 MTR_8g065030 38.406 414 235 7 48 452 53 455 3.87e-95 295
MS.gene67862.t1 MTR_2g103410 39.842 379 215 7 60 435 59 427 8.37e-89 278
MS.gene67862.t1 MTR_1g052315 43.333 360 185 10 54 407 57 403 8.84e-88 275
MS.gene67862.t1 MTR_5g034090 46.131 336 144 7 18 324 77 404 3.90e-87 272
MS.gene67862.t1 MTR_7g091850 41.620 358 196 5 59 413 75 422 5.54e-86 271
MS.gene67862.t1 MTR_7g091840 41.018 334 183 5 49 377 54 378 5.81e-81 255
MS.gene67862.t1 MTR_2g060350 39.394 363 205 7 54 412 68 419 7.66e-81 257
MS.gene67862.t1 MTR_6g028080 36.318 402 219 10 54 438 16 397 4.06e-74 238
MS.gene67862.t1 MTR_2g103650 34.545 385 240 7 60 440 28 404 4.66e-73 236
MS.gene67862.t1 MTR_1g012390 37.018 389 226 9 56 440 24 397 4.76e-72 233
MS.gene67862.t1 MTR_3g054320 35.623 393 225 10 60 446 30 400 2.51e-69 226
MS.gene67862.t1 MTR_3g030540 38.292 363 200 8 61 417 71 415 6.39e-69 226
MS.gene67862.t1 MTR_3g084380 34.916 358 213 6 60 412 21 363 7.47e-67 219
MS.gene67862.t1 MTR_2g049400 35.142 387 234 7 53 438 21 391 2.31e-66 218
MS.gene67862.t1 MTR_2g058670 35.227 352 216 7 59 407 24 366 8.15e-66 217
MS.gene67862.t1 MTR_8g010080 35.770 383 217 10 59 438 30 386 1.26e-64 213
MS.gene67862.t1 MTR_2g058640 36.261 353 211 8 59 407 24 366 1.33e-64 214
MS.gene67862.t1 MTR_4g084050 34.795 365 211 8 60 413 23 371 1.49e-64 213
MS.gene67862.t1 MTR_8g010070 34.536 388 225 11 57 440 28 390 7.34e-64 211
MS.gene67862.t1 MTR_8g010040 35.770 383 214 9 60 437 32 387 8.33e-64 211
MS.gene67862.t1 MTR_2g058840 34.540 359 207 8 59 407 24 364 9.80e-64 212
MS.gene67862.t1 MTR_3g438050 34.232 371 225 7 59 424 26 382 1.11e-63 211
MS.gene67862.t1 MTR_3g084360 34.540 359 215 8 60 413 24 367 3.58e-63 209
MS.gene67862.t1 MTR_1g088160 35.372 376 215 13 59 423 34 392 1.27e-62 208
MS.gene67862.t1 MTR_5g097860 38.037 326 185 7 59 379 24 337 1.73e-62 206
MS.gene67862.t1 MTR_5g043350 33.846 390 233 10 60 442 29 400 3.14e-62 207
MS.gene67862.t1 MTR_0157s0060 34.817 382 221 8 65 440 3 362 3.60e-62 206
MS.gene67862.t1 MTR_4g034020 34.817 382 221 8 65 440 3 362 3.60e-62 206
MS.gene67862.t1 MTR_8g008765 35.780 327 185 5 56 379 27 331 8.24e-62 204
MS.gene67862.t1 MTR_8g010110 33.247 385 229 8 57 438 28 387 2.23e-61 205
MS.gene67862.t1 MTR_4g083660 34.615 390 234 12 53 438 2 374 6.21e-61 203
MS.gene67862.t1 MTR_8g010090 33.506 385 228 9 57 438 28 387 3.41e-60 202
MS.gene67862.t1 MTR_7g035415 36.190 315 177 7 59 368 39 334 7.40e-60 199
MS.gene67862.t1 MTR_7g113910 35.772 369 150 8 59 425 56 339 1.08e-59 200
MS.gene67862.t1 MTR_2g435640 34.262 359 220 7 54 407 47 394 1.70e-58 198
MS.gene67862.t1 MTR_2g103410 37.453 267 157 4 60 324 59 317 3.75e-58 194
MS.gene67862.t1 MTR_8g009810 35.196 358 213 9 60 413 35 377 4.23e-57 194
MS.gene67862.t1 MTR_8g030930 33.983 359 215 9 59 409 26 370 8.70e-57 193
MS.gene67862.t1 MTR_1g013730 32.984 382 225 9 60 438 27 380 2.80e-55 189
MS.gene67862.t1 MTR_1g013740 32.552 384 228 9 60 440 35 390 3.16e-54 186
MS.gene67862.t1 MTR_4g120320 32.642 386 243 9 55 435 47 420 7.33e-54 186
MS.gene67862.t1 MTR_2g029170 35.000 340 202 10 102 438 38 361 4.57e-53 182
MS.gene67862.t1 MTR_8g010050 30.570 386 205 11 57 438 28 354 2.70e-52 180
MS.gene67862.t1 MTR_8g008700 33.746 323 167 8 118 438 13 290 1.13e-47 166
MS.gene67862.t1 MTR_8g077320 29.949 394 199 9 59 437 32 363 2.95e-47 167
MS.gene67862.t1 MTR_5g063430 31.169 308 186 8 59 359 24 312 1.81e-41 150
MS.gene67862.t1 MTR_8g031180 36.538 208 122 3 207 408 70 273 5.77e-39 142
MS.gene67862.t1 MTR_2g100040 31.129 363 182 13 59 415 24 324 1.49e-38 143
MS.gene67862.t1 MTR_4g124980 26.128 421 241 14 48 426 62 454 9.73e-22 98.2
MS.gene67862.t1 MTR_2g042740 25.722 381 236 12 58 407 54 418 5.95e-21 95.5
MS.gene67862.t1 MTR_3g449590 23.844 411 269 10 62 443 49 444 2.22e-17 84.7
MS.gene67862.t1 MTR_5g090160 25.879 313 191 9 65 349 49 348 3.11e-16 80.9
MS.gene67862.t1 MTR_1g100040 26.214 309 185 11 65 344 61 355 5.77e-15 77.4
MS.gene67862.t1 MTR_8g098840 24.571 350 210 13 62 376 46 376 6.44e-15 77.0
MS.gene67862.t1 MTR_2g435340 25.526 333 195 12 50 348 27 340 6.87e-15 77.0
MS.gene67862.t1 MTR_1g100050 30.000 230 131 7 65 276 55 272 9.99e-15 76.6
MS.gene67862.t1 MTR_7g088550 25.649 308 186 10 65 343 55 348 4.64e-14 74.3
MS.gene67862.t1 MTR_1g099850 25.243 309 188 10 65 344 60 354 1.34e-13 73.2
MS.gene67862.t1 MTR_1g100060 27.826 230 136 6 65 276 98 315 2.26e-13 72.4
MS.gene67862.t1 MTR_7g110710 26.006 323 192 10 62 353 45 351 2.66e-13 72.0
MS.gene67862.t1 MTR_7g110710 26.006 323 192 10 62 353 45 351 3.48e-13 71.6
MS.gene67862.t1 MTR_3g096330 29.000 200 116 6 158 344 189 375 5.45e-13 71.6
MS.gene67862.t1 MTR_8g028725 24.058 345 218 11 62 376 57 387 7.07e-13 70.9
MS.gene67862.t1 MTR_7g078680 23.614 415 265 12 65 446 60 455 1.37e-12 70.1
MS.gene67862.t1 MTR_1g086030 25.155 322 195 10 62 353 84 389 2.71e-12 68.9
MS.gene67862.t1 MTR_2g435340 27.230 213 132 6 148 348 74 275 9.22e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67862.t1 AT1G48100 71.269 449 122 3 4 448 21 466 0.0 658
MS.gene67862.t1 AT1G02460 62.148 391 143 3 59 444 88 478 0.0 519
MS.gene67862.t1 AT4G01890 56.281 398 169 2 48 440 53 450 3.50e-170 486
MS.gene67862.t1 AT1G56710 54.612 412 179 4 39 444 24 433 4.77e-168 480
MS.gene67862.t1 AT1G60590 45.499 411 191 6 60 443 123 527 4.67e-121 364
MS.gene67862.t1 AT1G10640 45.477 409 193 6 59 443 118 520 7.75e-120 360
MS.gene67862.t1 AT5G14650 45.679 405 204 6 53 454 44 435 5.42e-118 352
MS.gene67862.t1 AT3G26610 41.176 442 233 8 7 440 27 449 1.66e-107 327
MS.gene67862.t1 AT1G70500 41.162 396 220 6 59 452 66 450 5.63e-100 307
MS.gene67862.t1 AT1G80170 41.623 382 211 6 56 434 49 421 1.74e-99 305
MS.gene67862.t1 AT1G23460 41.927 384 206 7 59 438 42 412 3.62e-96 296
MS.gene67862.t1 AT1G23460 41.927 384 206 7 59 438 66 436 5.21e-96 297
MS.gene67862.t1 AT3G57510 41.714 350 189 6 62 407 70 408 1.97e-86 271
MS.gene67862.t1 AT2G41850 41.143 350 191 6 62 407 70 408 1.75e-85 269
MS.gene67862.t1 AT3G07970 39.257 377 211 9 51 423 64 426 5.13e-82 260
MS.gene67862.t1 AT5G14650 43.590 273 142 3 53 324 44 305 1.29e-72 231
MS.gene67862.t1 AT3G15720 37.984 387 213 10 60 439 23 389 5.99e-71 232
MS.gene67862.t1 AT3G15720 37.984 387 213 10 60 439 24 390 7.20e-71 231
MS.gene67862.t1 AT1G80140 36.286 350 205 6 92 438 2 336 8.10e-71 228
MS.gene67862.t1 AT4G35670 34.551 356 215 6 60 412 24 364 1.69e-69 226
MS.gene67862.t1 AT3G15720 37.984 387 210 11 60 439 23 386 2.42e-68 225
MS.gene67862.t1 AT2G43880 35.714 378 221 9 60 433 31 390 3.28e-66 218
MS.gene67862.t1 AT2G43870 37.892 351 201 7 60 407 21 357 5.74e-66 217
MS.gene67862.t1 AT1G80170 40.590 271 151 4 56 324 49 311 1.03e-65 214
MS.gene67862.t1 AT5G44840 33.067 375 225 8 59 426 23 378 1.46e-65 216
MS.gene67862.t1 AT1G05660 33.854 384 236 8 59 440 27 394 4.67e-65 214
MS.gene67862.t1 AT2G43890 35.638 376 224 11 60 433 29 388 2.63e-64 213
MS.gene67862.t1 AT1G05650 33.590 390 241 8 53 440 21 394 6.48e-64 211
MS.gene67862.t1 AT1G78400 35.417 384 232 6 59 439 34 404 7.19e-64 212
MS.gene67862.t1 AT1G65570 36.735 392 226 12 52 438 23 397 1.14e-63 211
MS.gene67862.t1 AT3G59850 36.827 353 203 10 60 407 23 360 1.89e-62 207
MS.gene67862.t1 AT3G59850 36.827 353 203 10 60 407 67 404 4.04e-62 208
MS.gene67862.t1 AT5G27530 33.889 360 208 7 59 413 52 386 4.62e-62 209
MS.gene67862.t1 AT1G17150 36.053 380 226 8 59 434 32 398 1.48e-61 206
MS.gene67862.t1 AT2G33160 36.170 376 219 9 40 407 11 373 4.57e-61 211
MS.gene67862.t1 AT1G02790 36.158 354 199 9 59 407 51 382 6.00e-61 204
MS.gene67862.t1 AT2G43860 35.844 385 226 12 60 440 33 400 8.82e-60 201
MS.gene67862.t1 AT5G48140 34.911 338 203 7 73 407 34 357 1.42e-59 200
MS.gene67862.t1 AT3G07830 36.686 338 198 7 73 407 34 358 2.03e-59 200
MS.gene67862.t1 AT3G07840 35.613 351 209 8 65 412 26 362 2.80e-59 199
MS.gene67862.t1 AT3G15720 37.209 344 190 9 102 439 3 326 2.20e-58 197
MS.gene67862.t1 AT4G13760 33.989 356 215 9 59 407 30 372 7.84e-57 193
MS.gene67862.t1 AT3G07820 35.860 343 203 8 73 412 33 361 1.32e-56 192
MS.gene67862.t1 AT1G43080 34.270 356 214 9 59 407 30 372 1.82e-56 192
MS.gene67862.t1 AT3G14040 37.396 361 208 8 52 408 63 409 3.56e-56 192
MS.gene67862.t1 AT1G43090 34.551 356 213 9 59 407 30 372 6.45e-56 192
MS.gene67862.t1 AT5G17200 31.785 409 233 10 56 440 35 421 7.81e-56 191
MS.gene67862.t1 AT2G43880 37.162 296 172 5 60 354 31 313 1.30e-55 188
MS.gene67862.t1 AT5G44830 31.765 340 202 6 90 426 7 319 1.59e-55 187
MS.gene67862.t1 AT5G39910 30.402 398 197 11 59 439 35 369 2.24e-55 189
MS.gene67862.t1 AT4G18180 35.574 357 209 9 59 407 38 381 2.78e-55 190
MS.gene67862.t1 AT4G32380 32.703 370 224 7 72 433 39 391 3.83e-55 190
MS.gene67862.t1 AT1G43100 34.454 357 212 10 59 407 30 372 2.40e-54 188
MS.gene67862.t1 AT3G07850 37.119 361 208 9 52 408 63 408 1.08e-53 186
MS.gene67862.t1 AT2G15460 34.358 358 211 11 59 407 30 372 1.55e-53 184
MS.gene67862.t1 AT2G26620 33.898 354 216 9 59 407 30 370 2.57e-53 184
MS.gene67862.t1 AT2G15450 34.078 358 212 11 59 407 30 372 4.74e-53 183
MS.gene67862.t1 AT2G40310 33.613 357 213 10 60 407 31 372 1.14e-52 182
MS.gene67862.t1 AT2G15470 33.799 358 213 11 59 407 30 372 1.65e-51 179
MS.gene67862.t1 AT4G32375 36.655 281 151 6 134 407 5 265 1.71e-51 180
MS.gene67862.t1 AT4G32370 35.836 293 166 6 74 358 49 327 3.58e-49 171
MS.gene67862.t1 AT4G35670 32.841 271 168 3 142 412 1 257 7.06e-49 169
MS.gene67862.t1 AT4G32380 35.688 269 152 5 72 335 39 291 6.67e-46 164
MS.gene67862.t1 AT3G42950 28.103 427 257 18 58 451 64 473 7.83e-22 98.2
MS.gene67862.t1 AT5G41870 27.103 321 189 9 59 349 44 349 5.52e-21 95.5
MS.gene67862.t1 AT1G19170 24.672 381 241 11 58 407 85 450 4.23e-20 93.2
MS.gene67862.t1 AT4G33440 26.168 321 190 10 55 344 67 371 8.17e-18 86.3
MS.gene67862.t1 AT3G62110 26.462 325 188 12 62 353 45 351 4.44e-17 84.0
MS.gene67862.t1 AT3G62110 26.462 325 188 12 62 353 45 351 4.44e-17 84.0
MS.gene67862.t1 AT4G23820 26.708 322 182 12 62 349 43 344 1.53e-16 82.0
MS.gene67862.t1 AT2G23900 23.980 392 242 14 65 422 61 430 7.47e-15 77.0
MS.gene67862.t1 AT3G48950 22.899 345 217 10 65 376 50 378 1.25e-14 76.3
MS.gene67862.t1 AT4G23500 24.096 415 265 13 65 447 73 469 2.64e-12 68.9
MS.gene67862.t1 AT3G06770 26.266 316 188 12 62 347 46 346 7.81e-11 64.3
MS.gene67862.t1 AT3G06770 26.266 316 188 12 62 347 45 345 7.86e-11 64.3

Find 107 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCTCCACTTGATTGTTTA+AGG 0.078821 2.4:+57794445 MS.gene67862:CDS
CTTGATCAAATGACTCTAAA+TGG 0.174861 2.4:+57792058 MS.gene67862:CDS
GGAGATTCTCCAGGATTATT+TGG 0.184472 2.4:-57791360 None:intergenic
GAATATGAATGCAATAATTA+AGG 0.201144 2.4:+57794501 MS.gene67862:CDS
GGTACCTTCCTTCCACATTA+TGG 0.224070 2.4:-57791233 None:intergenic
GATAACTCCACAACCGTTTC+AGG 0.228355 2.4:-57794477 None:intergenic
GTTCTTCATGAGCTCAAATT+TGG 0.241900 2.4:+57792402 MS.gene67862:CDS
CCATAAGCATTCCAACAAAA+AGG 0.243640 2.4:-57794402 None:intergenic
TCTCCAGGATTATTTGGATA+TGG 0.275950 2.4:-57791354 None:intergenic
GCAGCTTGTGCAGTTGATTC+AGG 0.281433 2.4:+57791471 MS.gene67862:CDS
GGATTATTTGGATATGGATA+TGG 0.286489 2.4:-57791348 None:intergenic
GTGCTCCTACCCACGGAATT+AGG 0.301522 2.4:+57792763 MS.gene67862:CDS
CAATTCCATGTGAAATTTGA+TGG 0.330222 2.4:+57792457 MS.gene67862:CDS
TCTGCCTCAGGCCAACAATT+TGG 0.335193 2.4:-57792004 None:intergenic
ATCTTCCTTGCAAACCTCAT+AGG 0.364129 2.4:+57792119 MS.gene67862:CDS
AGTTTCTTACATTCTCCATT+TGG 0.366739 2.4:-57794164 None:intergenic
TATCTTGTTTATAGATGATA+AGG 0.370877 2.4:+57792380 MS.gene67862:intron
TCCTCCACTTGATTGTTTAA+GGG 0.382641 2.4:+57794446 MS.gene67862:CDS
TTCGACAGCATTGGAAGCCT+TGG 0.386798 2.4:+57794006 MS.gene67862:intron
GGCCAACAATTTGGTCCATC+TGG 0.390506 2.4:-57791995 None:intergenic
AGCTCAAATTTGGTGTTAAG+AGG 0.391670 2.4:+57792412 MS.gene67862:CDS
TGACTGAGTTGCGGACTGTT+AGG 0.400859 2.4:-57794059 None:intergenic
ATTATTATTATTACCCTATG+AGG 0.401164 2.4:-57792133 None:intergenic
TCAGTATTGGGACTAAGTTT+TGG 0.402102 2.4:-57792520 None:intergenic
GGTGATGATTGCATTTCAAT+TGG 0.404860 2.4:+57792702 MS.gene67862:intron
TTATTTACCATTGCTTATCA+AGG 0.419053 2.4:-57792587 None:intergenic
AAGTGGATTCCATCAGTATT+GGG 0.420148 2.4:-57792532 None:intergenic
AATATGAATGCAATAATTAA+GGG 0.424968 2.4:+57794502 MS.gene67862:CDS
GGAGGTACAGGATCGGTAAC+AGG 0.426186 2.4:+57794123 MS.gene67862:CDS
CATCCCTTGTGTTTCCTAAG+TGG 0.427078 2.4:-57792549 None:intergenic
TTGAAATATATGTAGATTGA+TGG 0.430307 2.4:+57791959 MS.gene67862:intron
TTACATACACTGCTGATGTT+TGG 0.440754 2.4:-57794233 None:intergenic
TAAGTGGATTCCATCAGTAT+TGG 0.443227 2.4:-57792533 None:intergenic
ACTAGGAGATGTAGGAGGAT+TGG 0.444391 2.4:-57791313 None:intergenic
AACATTTGACAACATCCAAA+TGG 0.452717 2.4:+57794149 MS.gene67862:CDS
TCAAATTTCACATGGAATTG+AGG 0.453281 2.4:-57792454 None:intergenic
TTGATCAAATGACTCTAAAT+GGG 0.457341 2.4:+57792059 MS.gene67862:CDS
GTAGATTGATGGTACTTTGA+TGG 0.462628 2.4:+57791970 MS.gene67862:intron
GATTGCATTTCAATTGGTCC+TGG 0.464171 2.4:+57792708 MS.gene67862:CDS
GTAAGATCATTTGGAGCAGT+TGG 0.464835 2.4:+57791402 MS.gene67862:CDS
CACCAGATGGACCAAATTGT+TGG 0.467082 2.4:+57791993 MS.gene67862:CDS
CTTACTTGAAATACCAGTCC+TGG 0.467508 2.4:-57791561 None:intergenic
GGATTGGAAGAAGGTGGTGA+AGG 0.469757 2.4:-57791297 None:intergenic
AAAACATGGCAAGGAGGTAC+AGG 0.486274 2.4:+57794111 MS.gene67862:CDS
TCACTACAAGCAAAGTGTAT+TGG 0.494006 2.4:-57794306 None:intergenic
TATCCATATCCAAATAATCC+TGG 0.495114 2.4:+57791351 MS.gene67862:CDS
GGATATGGATATGGATCAGA+TGG 0.495462 2.4:-57791339 None:intergenic
TCAACTAATTCACCTTCATA+AGG 0.499997 2.4:-57794375 None:intergenic
AGGTGAGCCTGAAACGGTTG+TGG 0.500843 2.4:+57794470 MS.gene67862:CDS
TGGTCCATGCAAACCAGGAC+TGG 0.506011 2.4:+57791548 MS.gene67862:CDS
AATACCAGTCCTGGTTTGCA+TGG 0.514150 2.4:-57791552 None:intergenic
GATGTAGGAGGATTGGAAGA+AGG 0.516798 2.4:-57791306 None:intergenic
TCTTCCTTGCAAACCTCATA+GGG 0.518124 2.4:+57792120 MS.gene67862:CDS
GGTGAAGGGTCACTAGGTGC+TGG 0.521610 2.4:-57791282 None:intergenic
TAATCCTGGAGAATCTCCTT+CGG 0.522281 2.4:+57791365 MS.gene67862:CDS
AAACTTAGTCCCAATACTGA+TGG 0.523177 2.4:+57792523 MS.gene67862:CDS
AATACTGATGGAATCCACTT+AGG 0.526638 2.4:+57792535 MS.gene67862:CDS
CAAATGACTCTAAATGGGAC+AGG 0.527686 2.4:+57792064 MS.gene67862:CDS
CCACTTGATTGTTTAAGGGA+AGG 0.528218 2.4:+57794450 MS.gene67862:CDS
GATGGAACACTAGGAGATGT+AGG 0.530462 2.4:-57791321 None:intergenic
AATGGGACAGGAATTATTGA+AGG 0.532465 2.4:+57792076 MS.gene67862:CDS
GATTGGAAGAAGGTGGTGAA+GGG 0.533569 2.4:-57791296 None:intergenic
AAGGGAAGGTGAGCCTGAAA+CGG 0.547901 2.4:+57794464 MS.gene67862:CDS
GTCTATAAAACACAAGCCAT+TGG 0.548487 2.4:-57792036 None:intergenic
TCATTTGGAGCAGTTGGAGA+TGG 0.548866 2.4:+57791408 MS.gene67862:CDS
CTACAAGCAAAGTGTATTGG+AGG 0.549583 2.4:-57794303 None:intergenic
ACAGGAATTATTGAAGGCAA+TGG 0.554020 2.4:+57792082 MS.gene67862:CDS
GAAGGTGGTGAAGGGTCACT+AGG 0.554808 2.4:-57791288 None:intergenic
GCTCAAATTTGGTGTTAAGA+GGG 0.555873 2.4:+57792413 MS.gene67862:CDS
GTAGGAGGATTGGAAGAAGG+TGG 0.559194 2.4:-57791303 None:intergenic
TTAGGAAACACAAGGGATGT+TGG 0.561840 2.4:+57792553 MS.gene67862:CDS
TATAATTCCTTGATAAGCAA+TGG 0.563522 2.4:+57792580 MS.gene67862:CDS
GTGAAATTTGATGGTTGTCA+AGG 0.563909 2.4:+57792466 MS.gene67862:CDS
ATGTGACATACCTAATTCCG+TGG 0.569578 2.4:-57792773 None:intergenic
CAATCCGAAGGAGATTCTCC+AGG 0.577573 2.4:-57791369 None:intergenic
ACATCGAAAACGCAATCCGA+AGG 0.582946 2.4:-57791381 None:intergenic
TATGGATCAGATGGAACACT+AGG 0.584081 2.4:-57791330 None:intergenic
TCATCCATAATGTGGAAGGA+AGG 0.585473 2.4:+57791229 MS.gene67862:CDS
GTTACTTGTGCTCCTACCCA+CGG 0.586560 2.4:+57792756 MS.gene67862:CDS
ATTACCCTATGAGGTTTGCA+AGG 0.592983 2.4:-57792124 None:intergenic
CTAAGAATCAAAACATGGCA+AGG 0.597482 2.4:+57794102 MS.gene67862:CDS
AAGGCAATGGAGAACAATGG+TGG 0.608620 2.4:+57792095 MS.gene67862:CDS
ACATTCACATCTGAACATCC+AGG 0.609157 2.4:-57792726 None:intergenic
CGTGGGAATTGTGTACTCCA+AGG 0.610752 2.4:-57794023 None:intergenic
TGTTCAGATGTGAATGTGGA+TGG 0.618209 2.4:+57792732 MS.gene67862:CDS
GTCATAAAAGAATCAGACAA+TGG 0.619796 2.4:+57794078 MS.gene67862:CDS
TCCCTTAAACAATCAAGTGG+AGG 0.624213 2.4:-57794447 None:intergenic
AGGCAATGGAGAACAATGGT+GGG 0.624461 2.4:+57792096 MS.gene67862:CDS
GACAACCATCAAATTTCACA+TGG 0.633858 2.4:-57792462 None:intergenic
AGGCAGACAGTAAAAGCCAA+TGG 0.642020 2.4:+57792020 MS.gene67862:CDS
CCTTCCCTTAAACAATCAAG+TGG 0.643008 2.4:-57794450 None:intergenic
GGTACTTTGATGGCACCAGA+TGG 0.645534 2.4:+57791980 MS.gene67862:CDS
TGGACCAAATTGTTGGCCTG+AGG 0.650727 2.4:+57792000 MS.gene67862:CDS
ACATACCTAATTCCGTGGGT+AGG 0.654879 2.4:-57792768 None:intergenic
GAATCCACTTAGGAAACACA+AGG 0.655820 2.4:+57792545 MS.gene67862:CDS
TGGATGTTCAGATGTGAATG+TGG 0.656226 2.4:+57792728 MS.gene67862:CDS
TAGGTTTGAGACACATGCGT+GGG 0.660709 2.4:-57794040 None:intergenic
TTGAAGGCAATGGAGAACAA+TGG 0.660810 2.4:+57792092 MS.gene67862:CDS
TTAGGTTTGAGACACATGCG+TGG 0.673355 2.4:-57794041 None:intergenic
CTCACTGTAGGGCTAACACA+TGG 0.682946 2.4:-57792265 None:intergenic
CAGCTGCATTTAGAGCAGCA+TGG 0.685070 2.4:+57791445 MS.gene67862:CDS
TGTGACATACCTAATTCCGT+GGG 0.685322 2.4:-57792772 None:intergenic
ATGGCAAGGAGGTACAGGAT+CGG 0.689477 2.4:+57794116 MS.gene67862:CDS
GGAACACTAGGAGATGTAGG+AGG 0.693701 2.4:-57791318 None:intergenic
ATGGACTAAGAATCAAAACA+TGG 0.703229 2.4:+57794097 MS.gene67862:CDS
AGAATCAAAACATGGCAAGG+AGG 0.705826 2.4:+57794105 MS.gene67862:CDS
AATCCACTTAGGAAACACAA+GGG 0.771508 2.4:+57792546 MS.gene67862:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAGAAAAATAAATAAATAA+TGG + chr2.4:57793030-57793049 MS.gene67862:intron 10.0%
!! CAATTAATTATGATTAATTT+AGG + chr2.4:57792834-57792853 MS.gene67862:intron 10.0%
!! TCATAAGAAAAAAAATTATA+AGG - chr2.4:57792358-57792377 None:intergenic 10.0%
!!! AATATAGAATTTTTATTCTA+TGG + chr2.4:57792634-57792653 MS.gene67862:intron 10.0%
!!! AATGTTACATTTTATTTAAT+AGG + chr2.4:57792681-57792700 MS.gene67862:intron 10.0%
!!! TTTTTTCTTCAAATTTATTT+TGG + chr2.4:57793835-57793854 MS.gene67862:intron 10.0%
!! AACTTAATCTTAAAAACAAT+TGG - chr2.4:57793488-57793507 None:intergenic 15.0%
!! TCATAAAATGATTACATAAT+TGG - chr2.4:57793779-57793798 None:intergenic 15.0%
!!! AATTAAATTTTACACAATGA+TGG - chr2.4:57791916-57791935 None:intergenic 15.0%
!!! ATTTTAAAACAGTAATTTTC+AGG - chr2.4:57791510-57791529 None:intergenic 15.0%
!!! TAAAACTGATTTTATATGTT+TGG + chr2.4:57793387-57793406 MS.gene67862:intron 15.0%
!!! TCTGTTATTTTAGTTAAATT+GGG + chr2.4:57792800-57792819 MS.gene67862:intron 15.0%
!!! TTCTGTTATTTTAGTTAAAT+TGG + chr2.4:57792799-57792818 MS.gene67862:intron 15.0%
!!! TTTTCTTTTCAAGTTTATTT+TGG - chr2.4:57793657-57793676 None:intergenic 15.0%
!! ACCTTGTTAGTTAATTATAT+AGG - chr2.4:57791940-57791959 None:intergenic 20.0%
!! ATTATTATTATTACCCTATG+AGG - chr2.4:57792136-57792155 None:intergenic 20.0%
!! TCCTATATAATTAACTAACA+AGG + chr2.4:57791936-57791955 MS.gene67862:intron 20.0%
!! TTGAAATATATGTAGATTGA+TGG + chr2.4:57791959-57791978 MS.gene67862:intron 20.0%
!!! ACATCTTCTTAGAATTATTT+TGG + chr2.4:57793868-57793887 MS.gene67862:intron 20.0%
!!! ATCACAGTTTATTATTTTAG+AGG + chr2.4:57793362-57793381 MS.gene67862:intron 20.0%
!!! CTTTAAATTTTAGACAAATG+TGG + chr2.4:57791629-57791648 MS.gene67862:intron 20.0%
!!! TAAATTTCCTTTTATGTTCA+CGG + chr2.4:57791593-57791612 MS.gene67862:intron 20.0%
!!! TATCTTGTTTATAGATGATA+AGG + chr2.4:57792380-57792399 MS.gene67862:intron 20.0%
! AAAAAGAAACTGTGATGTAA+TGG - chr2.4:57792315-57792334 None:intergenic 25.0%
! ACTATTGTCACATTAATGAT+TGG - chr2.4:57793169-57793188 None:intergenic 25.0%
! ATCTCCTACAGAAAAATAAA+GGG + chr2.4:57794261-57794280 MS.gene67862:CDS 25.0%
! GTGATCATTTGATGTTAAAA+AGG - chr2.4:57793079-57793098 None:intergenic 25.0%
! TATAATTCCTTGATAAGCAA+TGG + chr2.4:57792580-57792599 MS.gene67862:CDS 25.0%
! TATCTCCTACAGAAAAATAA+AGG + chr2.4:57794260-57794279 MS.gene67862:CDS 25.0%
! TTATTTACCATTGCTTATCA+AGG - chr2.4:57792590-57792609 None:intergenic 25.0%
! TTGATCAAATGACTCTAAAT+GGG + chr2.4:57792059-57792078 MS.gene67862:CDS 25.0%
! TTTAACATCAAATGATCACA+GGG + chr2.4:57793080-57793099 MS.gene67862:intron 25.0%
!! ATTGAACTTTTACCTTATGA+AGG + chr2.4:57794363-57794382 MS.gene67862:CDS 25.0%
!! CTTTTGATAAACAGTAGAAT+TGG - chr2.4:57794203-57794222 None:intergenic 25.0%
!! TGAATATTTTACTCACTGTA+GGG - chr2.4:57792279-57792298 None:intergenic 25.0%
!! TTTTAACATCAAATGATCAC+AGG + chr2.4:57793079-57793098 MS.gene67862:intron 25.0%
!!! AGAAGTTGTTTTATGTTGTA+TGG + chr2.4:57793746-57793765 MS.gene67862:intron 25.0%
!!! TTGCCTTTTGAAATGATTTT+TGG - chr2.4:57792931-57792950 None:intergenic 25.0%
AAACTGTGATGTAATGGTTA+AGG - chr2.4:57792309-57792328 None:intergenic 30.0%
AACATTTGACAACATCCAAA+TGG + chr2.4:57794149-57794168 MS.gene67862:CDS 30.0%
AGTTTCTTACATTCTCCATT+TGG - chr2.4:57794167-57794186 None:intergenic 30.0%
ATAAAAGTTTGCAGCGTAAT+TGG + chr2.4:57791872-57791891 MS.gene67862:intron 30.0%
ATATATACACCCAAATTCTG+TGG + chr2.4:57792904-57792923 MS.gene67862:intron 30.0%
CAATTCCATGTGAAATTTGA+TGG + chr2.4:57792457-57792476 MS.gene67862:CDS 30.0%
CATAAGCATTCCAACAAAAA+GGG - chr2.4:57794404-57794423 None:intergenic 30.0%
CTTGATCAAATGACTCTAAA+TGG + chr2.4:57792058-57792077 MS.gene67862:CDS 30.0%
CTTGCTACTCAAAATTCTAT+AGG - chr2.4:57793947-57793966 None:intergenic 30.0%
GACTATAGTACTTTAACAGT+TGG + chr2.4:57793130-57793149 MS.gene67862:intron 30.0%
GGATTATTTGGATATGGATA+TGG - chr2.4:57791351-57791370 None:intergenic 30.0%
GGTCCAAAAATCATTTCAAA+AGG + chr2.4:57792925-57792944 MS.gene67862:intron 30.0%
GTCATAAAAGAATCAGACAA+TGG + chr2.4:57794078-57794097 MS.gene67862:CDS 30.0%
TAAGCTACAAACTTAGTTGA+TGG + chr2.4:57792870-57792889 MS.gene67862:intron 30.0%
TATCCATATCCAAATAATCC+TGG + chr2.4:57791351-57791370 MS.gene67862:CDS 30.0%
TCAAATTTCACATGGAATTG+AGG - chr2.4:57792457-57792476 None:intergenic 30.0%
TCAACTAATTCACCTTCATA+AGG - chr2.4:57794378-57794397 None:intergenic 30.0%
! ATCACTTCTACTATCTTTTC+TGG + chr2.4:57791528-57791547 MS.gene67862:CDS 30.0%
! ATGGACTAAGAATCAAAACA+TGG + chr2.4:57794097-57794116 MS.gene67862:CDS 30.0%
! ATTCTTTTATGACTGAGTTG+CGG - chr2.4:57794071-57794090 None:intergenic 30.0%
! CTGAATATTTTACTCACTGT+AGG - chr2.4:57792280-57792299 None:intergenic 30.0%
! GATTCTTTTCATCCATAATG+TGG + chr2.4:57791221-57791240 MS.gene67862:CDS 30.0%
! GTTTTCGATGTAAGATCATT+TGG + chr2.4:57791393-57791412 MS.gene67862:CDS 30.0%
! TTTTCATTTCAGAGTCCAAA+TGG + chr2.4:57792226-57792245 MS.gene67862:intron 30.0%
!! ATTTTTGGACCACAGAATTT+GGG - chr2.4:57792916-57792935 None:intergenic 30.0%
!! TTTTCTTTGGTTTCTTGTGA+TGG - chr2.4:57791257-57791276 None:intergenic 30.0%
!!! ACGATTGTGGTTTTAAATTG+TGG + chr2.4:57791739-57791758 MS.gene67862:intron 30.0%
!!! TGTACCCTTTATTTTTCTGT+AGG - chr2.4:57794268-57794287 None:intergenic 30.0%
!!! TTACATGTGATGTGCTATTT+TGG + chr2.4:57793919-57793938 MS.gene67862:intron 30.0%
!!! TTACCTTGGGACTAATTTTT+TGG + chr2.4:57793245-57793264 MS.gene67862:intron 30.0%
AAACTTAGTCCCAATACTGA+TGG + chr2.4:57792523-57792542 MS.gene67862:CDS 35.0%
AAGGTAATAGATTGCTACCT+AGG - chr2.4:57793232-57793251 None:intergenic 35.0%
AATACTGATGGAATCCACTT+AGG + chr2.4:57792535-57792554 MS.gene67862:CDS 35.0%
AATCCACTTAGGAAACACAA+GGG + chr2.4:57792546-57792565 MS.gene67862:CDS 35.0%
AATGGGACAGGAATTATTGA+AGG + chr2.4:57792076-57792095 MS.gene67862:CDS 35.0%
ACAGGAATTATTGAAGGCAA+TGG + chr2.4:57792082-57792101 MS.gene67862:CDS 35.0%
AGGTAGCAATCTATTACCTT+GGG + chr2.4:57793232-57793251 MS.gene67862:intron 35.0%
AGTTATTACACTCACACTAC+AGG - chr2.4:57792976-57792995 None:intergenic 35.0%
CCAAATGGAAAAACTGTATC+AGG + chr2.4:57792241-57792260 MS.gene67862:CDS 35.0%
CCATAAGCATTCCAACAAAA+AGG - chr2.4:57794405-57794424 None:intergenic 35.0%
CTAAGAATCAAAACATGGCA+AGG + chr2.4:57794102-57794121 MS.gene67862:CDS 35.0%
GACAACCATCAAATTTCACA+TGG - chr2.4:57792465-57792484 None:intergenic 35.0%
GGTGATGATTGCATTTCAAT+TGG + chr2.4:57792702-57792721 MS.gene67862:intron 35.0%
GTCTATAAAACACAAGCCAT+TGG - chr2.4:57792039-57792058 None:intergenic 35.0%
GTGAGTGTAATAACTCTTGA+AGG + chr2.4:57792981-57793000 MS.gene67862:intron 35.0%
GTTCTTCATGAGCTCAAATT+TGG + chr2.4:57792402-57792421 MS.gene67862:CDS 35.0%
TAGGTAGCAATCTATTACCT+TGG + chr2.4:57793231-57793250 MS.gene67862:intron 35.0%
TCACTACAAGCAAAGTGTAT+TGG - chr2.4:57794309-57794328 None:intergenic 35.0%
TCCTCCACTTGATTGTTTAA+GGG + chr2.4:57794446-57794465 MS.gene67862:CDS 35.0%
TCTCCAGGATTATTTGGATA+TGG - chr2.4:57791357-57791376 None:intergenic 35.0%
TGTCCAAAAAATTAGTCCCA+AGG - chr2.4:57793251-57793270 None:intergenic 35.0%
TTACATACACTGCTGATGTT+TGG - chr2.4:57794236-57794255 None:intergenic 35.0%
TTCCTCCACTTGATTGTTTA+AGG + chr2.4:57794445-57794464 MS.gene67862:CDS 35.0%
! AAGTGGATTCCATCAGTATT+GGG - chr2.4:57792535-57792554 None:intergenic 35.0%
! CCTGATACAGTTTTTCCATT+TGG - chr2.4:57792244-57792263 None:intergenic 35.0%
! CCTTTTTGTTGGAATGCTTA+TGG + chr2.4:57794402-57794421 MS.gene67862:CDS 35.0%
! CGCTGCAAACTTTTATATTG+CGG - chr2.4:57791868-57791887 None:intergenic 35.0%
! CTTTTCATCCATAATGTGGA+AGG + chr2.4:57791225-57791244 MS.gene67862:CDS 35.0%
! GCAATTTAGAATCACGATTG+TGG + chr2.4:57791726-57791745 MS.gene67862:intron 35.0%
! GTAGATTGATGGTACTTTGA+TGG + chr2.4:57791970-57791989 MS.gene67862:intron 35.0%
! GTGAAATTTGATGGTTGTCA+AGG + chr2.4:57792466-57792485 MS.gene67862:CDS 35.0%
! TAAGTGGATTCCATCAGTAT+TGG - chr2.4:57792536-57792555 None:intergenic 35.0%
!! ACTAAGTTTTGGTGCAGAAA+TGG - chr2.4:57792512-57792531 None:intergenic 35.0%
!! AGCTCAAATTTGGTGTTAAG+AGG + chr2.4:57792412-57792431 MS.gene67862:CDS 35.0%
!! GATTTTTGGACCACAGAATT+TGG - chr2.4:57792917-57792936 None:intergenic 35.0%
!! GCTCAAATTTGGTGTTAAGA+GGG + chr2.4:57792413-57792432 MS.gene67862:CDS 35.0%
!! TCAGTATTGGGACTAAGTTT+TGG - chr2.4:57792523-57792542 None:intergenic 35.0%
!!! TAGTTGATGACCCTTTTTGT+TGG + chr2.4:57794391-57794410 MS.gene67862:CDS 35.0%
AACGCAACCGTGAACATAAA+AGG - chr2.4:57791603-57791622 None:intergenic 40.0%
ACATTCACATCTGAACATCC+AGG - chr2.4:57792729-57792748 None:intergenic 40.0%
AGAATCAAAACATGGCAAGG+AGG + chr2.4:57794105-57794124 MS.gene67862:CDS 40.0%
ATCTTCCTTGCAAACCTCAT+AGG + chr2.4:57792119-57792138 MS.gene67862:CDS 40.0%
ATGTGACATACCTAATTCCG+TGG - chr2.4:57792776-57792795 None:intergenic 40.0%
ATTACCCTATGAGGTTTGCA+AGG - chr2.4:57792127-57792146 None:intergenic 40.0%
CAAATGACTCTAAATGGGAC+AGG + chr2.4:57792064-57792083 MS.gene67862:CDS 40.0%
CCACTTGATTGTTTAAGGGA+AGG + chr2.4:57794450-57794469 MS.gene67862:CDS 40.0%
CCTTCCCTTAAACAATCAAG+TGG - chr2.4:57794453-57794472 None:intergenic 40.0%
CTACAAGCAAAGTGTATTGG+AGG - chr2.4:57794306-57794325 None:intergenic 40.0%
CTGTCGAAATCACATCAGTA+AGG - chr2.4:57793994-57794013 None:intergenic 40.0%
CTTACTTGAAATACCAGTCC+TGG - chr2.4:57791564-57791583 None:intergenic 40.0%
CTTCAAAATCCTTTACGCTG+CGG - chr2.4:57791784-57791803 None:intergenic 40.0%
GAATCCACTTAGGAAACACA+AGG + chr2.4:57792545-57792564 MS.gene67862:CDS 40.0%
GATTGCATTTCAATTGGTCC+TGG + chr2.4:57792708-57792727 MS.gene67862:CDS 40.0%
GCACATCACATGTAACTTCT+AGG - chr2.4:57793914-57793933 None:intergenic 40.0%
GGAGATTCTCCAGGATTATT+TGG - chr2.4:57791363-57791382 None:intergenic 40.0%
GGATATGGATATGGATCAGA+TGG - chr2.4:57791342-57791361 None:intergenic 40.0%
TAATCCTGGAGAATCTCCTT+CGG + chr2.4:57791365-57791384 MS.gene67862:CDS 40.0%
TATGGATCAGATGGAACACT+AGG - chr2.4:57791333-57791352 None:intergenic 40.0%
TCATCCATAATGTGGAAGGA+AGG + chr2.4:57791229-57791248 MS.gene67862:CDS 40.0%
TCCCTTAAACAATCAAGTGG+AGG - chr2.4:57794450-57794469 None:intergenic 40.0%
TCTTCCTTGCAAACCTCATA+GGG + chr2.4:57792120-57792139 MS.gene67862:CDS 40.0%
TGATGTGATTTCGACAGCAT+TGG + chr2.4:57793997-57794016 MS.gene67862:intron 40.0%
TGGATGTTCAGATGTGAATG+TGG + chr2.4:57792728-57792747 MS.gene67862:CDS 40.0%
TGTGACATACCTAATTCCGT+GGG - chr2.4:57792775-57792794 None:intergenic 40.0%
TGTGTACTTCTCATCAACTG+TGG - chr2.4:57793582-57793601 None:intergenic 40.0%
TGTTCAGATGTGAATGTGGA+TGG + chr2.4:57792732-57792751 MS.gene67862:CDS 40.0%
TTAGACAAATGTGGCTGATG+TGG + chr2.4:57791638-57791657 MS.gene67862:intron 40.0%
TTAGGAAACACAAGGGATGT+TGG + chr2.4:57792553-57792572 MS.gene67862:CDS 40.0%
TTGAAGGCAATGGAGAACAA+TGG + chr2.4:57792092-57792111 MS.gene67862:CDS 40.0%
! GATGAGAAGTACACACTTAG+TGG + chr2.4:57793587-57793606 MS.gene67862:intron 40.0%
! GTAAGATCATTTGGAGCAGT+TGG + chr2.4:57791402-57791421 MS.gene67862:CDS 40.0%
AAGGCAATGGAGAACAATGG+TGG + chr2.4:57792095-57792114 MS.gene67862:CDS 45.0%
ACATACCTAATTCCGTGGGT+AGG - chr2.4:57792771-57792790 None:intergenic 45.0%
ACATCGAAAACGCAATCCGA+AGG - chr2.4:57791384-57791403 None:intergenic 45.0%
ACTAGGAGATGTAGGAGGAT+TGG - chr2.4:57791316-57791335 None:intergenic 45.0%
AGGCAATGGAGAACAATGGT+GGG + chr2.4:57792096-57792115 MS.gene67862:CDS 45.0%
AGGCAGACAGTAAAAGCCAA+TGG + chr2.4:57792020-57792039 MS.gene67862:CDS 45.0%
ATAGATTGCTACCTAGGAGC+AGG - chr2.4:57793226-57793245 None:intergenic 45.0%
CACCAGATGGACCAAATTGT+TGG + chr2.4:57791993-57792012 MS.gene67862:CDS 45.0%
CATCCCTTGTGTTTCCTAAG+TGG - chr2.4:57792552-57792571 None:intergenic 45.0%
GATAACTCCACAACCGTTTC+AGG - chr2.4:57794480-57794499 None:intergenic 45.0%
GATGGAACACTAGGAGATGT+AGG - chr2.4:57791324-57791343 None:intergenic 45.0%
GATGTAGGAGGATTGGAAGA+AGG - chr2.4:57791309-57791328 None:intergenic 45.0%
GATTGGAAGAAGGTGGTGAA+GGG - chr2.4:57791299-57791318 None:intergenic 45.0%
GGCTGCAATTATGGTTGCAA+AGG + chr2.4:57791659-57791678 MS.gene67862:intron 45.0%
GGTACCTTCCTTCCACATTA+TGG - chr2.4:57791236-57791255 None:intergenic 45.0%
GTGGTCTGCAACAGTAATAG+AGG + chr2.4:57791758-57791777 MS.gene67862:intron 45.0%
TAGATTGCTACCTAGGAGCA+GGG - chr2.4:57793225-57793244 None:intergenic 45.0%
! AAAACATGGCAAGGAGGTAC+AGG + chr2.4:57794111-57794130 MS.gene67862:CDS 45.0%
! AATACCAGTCCTGGTTTGCA+TGG - chr2.4:57791555-57791574 None:intergenic 45.0%
! CTGCAGAAACTTTTACGCTG+TGG + chr2.4:57791682-57791701 MS.gene67862:intron 45.0%
! TAGGTTTGAGACACATGCGT+GGG - chr2.4:57794043-57794062 None:intergenic 45.0%
! TCATTTGGAGCAGTTGGAGA+TGG + chr2.4:57791408-57791427 MS.gene67862:CDS 45.0%
! TTAGGTTTGAGACACATGCG+TGG - chr2.4:57794044-57794063 None:intergenic 45.0%
!! GGTGCTGGAGAAGTTTTCTT+TGG - chr2.4:57791270-57791289 None:intergenic 45.0%
!! TTTTCTGGTCCATGCAAACC+AGG + chr2.4:57791543-57791562 MS.gene67862:CDS 45.0%
AAGGGAAGGTGAGCCTGAAA+CGG + chr2.4:57794464-57794483 MS.gene67862:CDS 50.0%
AGATTGCTACCTAGGAGCAG+GGG - chr2.4:57793224-57793243 None:intergenic 50.0%
CAATCCGAAGGAGATTCTCC+AGG - chr2.4:57791372-57791391 None:intergenic 50.0%
CGTGGGAATTGTGTACTCCA+AGG - chr2.4:57794026-57794045 None:intergenic 50.0%
CTCACTGTAGGGCTAACACA+TGG - chr2.4:57792268-57792287 None:intergenic 50.0%
GGAACACTAGGAGATGTAGG+AGG - chr2.4:57791321-57791340 None:intergenic 50.0%
GGATTGGAAGAAGGTGGTGA+AGG - chr2.4:57791300-57791319 None:intergenic 50.0%
GGCCAACAATTTGGTCCATC+TGG - chr2.4:57791998-57792017 None:intergenic 50.0%
GGCTGATGTGGCTGCAATTA+TGG + chr2.4:57791650-57791669 MS.gene67862:intron 50.0%
GTAGGAGGATTGGAAGAAGG+TGG - chr2.4:57791306-57791325 None:intergenic 50.0%
GTTACTTGTGCTCCTACCCA+CGG + chr2.4:57792756-57792775 MS.gene67862:CDS 50.0%
TAATAGAGGCCGCAGCGTAA+AGG + chr2.4:57791772-57791791 MS.gene67862:intron 50.0%
TCTGCCTCAGGCCAACAATT+TGG - chr2.4:57792007-57792026 None:intergenic 50.0%
! ATGGCAAGGAGGTACAGGAT+CGG + chr2.4:57794116-57794135 MS.gene67862:CDS 50.0%
! CAGCTGCATTTAGAGCAGCA+TGG + chr2.4:57791445-57791464 MS.gene67862:CDS 50.0%
! GCAGCTTGTGCAGTTGATTC+AGG + chr2.4:57791471-57791490 MS.gene67862:CDS 50.0%
! GGTACTTTGATGGCACCAGA+TGG + chr2.4:57791980-57791999 MS.gene67862:CDS 50.0%
! TGACTGAGTTGCGGACTGTT+AGG - chr2.4:57794062-57794081 None:intergenic 50.0%
! TGGACCAAATTGTTGGCCTG+AGG + chr2.4:57792000-57792019 MS.gene67862:CDS 50.0%
! TGGCTTTTACTGTCTGCCTC+AGG - chr2.4:57792019-57792038 None:intergenic 50.0%
! TTCGACAGCATTGGAAGCCT+TGG + chr2.4:57794006-57794025 MS.gene67862:intron 50.0%
AGGTGAGCCTGAAACGGTTG+TGG + chr2.4:57794470-57794489 MS.gene67862:CDS 55.0%
GAAGGTGGTGAAGGGTCACT+AGG - chr2.4:57791291-57791310 None:intergenic 55.0%
GTGCTCCTACCCACGGAATT+AGG + chr2.4:57792763-57792782 MS.gene67862:CDS 55.0%
TGGTCCATGCAAACCAGGAC+TGG + chr2.4:57791548-57791567 MS.gene67862:CDS 55.0%
! CTGTTTTGTCCCCTGCTCCT+AGG + chr2.4:57793212-57793231 MS.gene67862:intron 55.0%
! GGAGGTACAGGATCGGTAAC+AGG + chr2.4:57794123-57794142 MS.gene67862:CDS 55.0%
GGTGAAGGGTCACTAGGTGC+TGG - chr2.4:57791285-57791304 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 57791219 57794521 57791219 ID=MS.gene67862
chr2.4 mRNA 57791219 57794521 57791219 ID=MS.gene67862.t1;Parent=MS.gene67862
chr2.4 exon 57791219 57791578 57791219 ID=MS.gene67862.t1.exon1;Parent=MS.gene67862.t1
chr2.4 CDS 57791219 57791578 57791219 ID=cds.MS.gene67862.t1;Parent=MS.gene67862.t1
chr2.4 exon 57791974 57792141 57791974 ID=MS.gene67862.t1.exon2;Parent=MS.gene67862.t1
chr2.4 CDS 57791974 57792141 57791974 ID=cds.MS.gene67862.t1;Parent=MS.gene67862.t1
chr2.4 exon 57792238 57792282 57792238 ID=MS.gene67862.t1.exon3;Parent=MS.gene67862.t1
chr2.4 CDS 57792238 57792282 57792238 ID=cds.MS.gene67862.t1;Parent=MS.gene67862.t1
chr2.4 exon 57792394 57792601 57792394 ID=MS.gene67862.t1.exon4;Parent=MS.gene67862.t1
chr2.4 CDS 57792394 57792601 57792394 ID=cds.MS.gene67862.t1;Parent=MS.gene67862.t1
chr2.4 exon 57792703 57792784 57792703 ID=MS.gene67862.t1.exon5;Parent=MS.gene67862.t1
chr2.4 CDS 57792703 57792784 57792703 ID=cds.MS.gene67862.t1;Parent=MS.gene67862.t1
chr2.4 exon 57794014 57794521 57794014 ID=MS.gene67862.t1.exon6;Parent=MS.gene67862.t1
chr2.4 CDS 57794014 57794521 57794014 ID=cds.MS.gene67862.t1;Parent=MS.gene67862.t1
Gene Sequence

>MS.gene67862

ATGATTCTTTTCATCCATAATGTGGAAGGAAGGTACCATCACAAGAAACCAAAGAAAACTTCTCCAGCACCTAGTGACCCTTCACCACCTTCTTCCAATCCTCCTACATCTCCTAGTGTTCCATCTGATCCATATCCATATCCAAATAATCCTGGAGAATCTCCTTCGGATTGCGTTTTCGATGTAAGATCATTTGGAGCAGTTGGAGATGGTAATGCTGATGATACAGCTGCATTTAGAGCAGCATGGAAAGCAGCTTGTGCAGTTGATTCAGGAGTTCTTTTAGCTCCTGAAAATTACTGTTTTAAAATCACTTCTACTATCTTTTCTGGTCCATGCAAACCAGGACTGGTATTTCAAGTAAGAATGTTTCTTAAATTTCCTTTTATGTTCACGGTTGCGTTGCGATTCTTTAAATTTTAGACAAATGTGGCTGATGTGGCTGCAATTATGGTTGCAAAGGCTGCAGAAACTTTTACGCTGTGGTCGCAATTGCTGTTATAGACTGCAATTTAGAATCACGATTGTGGTTTTAAATTGTGGTCTGCAACAGTAATAGAGGCCGCAGCGTAAAGGATTTTGAAGTCTCTTCAAAGTCATTGTAACTATAATTTTGACTGCATCAGCTGCATTTTCTCTAATTTATCCGCAATATAAAAGTTTGCAGCGTAATTGGAACTGAAATTTAACATGACCATCATTGTGTAAAATTTAATTTCCTATATAATTAACTAACAAGGTTGAAATATATGTAGATTGATGGTACTTTGATGGCACCAGATGGACCAAATTGTTGGCCTGAGGCAGACAGTAAAAGCCAATGGCTTGTGTTTTATAGACTTGATCAAATGACTCTAAATGGGACAGGAATTATTGAAGGCAATGGAGAACAATGGTGGGATCTTCCTTGCAAACCTCATAGGGTAATAATAATAATTATTATTAAAACTTTATTTTTTTTTATTTATTTATCTAATGTTAAAAACTAAAATTCTAGTAATTTTTCATTTTCATTTCAGAGTCCAAATGGAAAAACTGTATCAGGACCATGTGTTAGCCCTACAGTGAGTAAAATATTCAGAATTTACCTTAACCATTACATCACAGTTTCTTTTTTTTTTTTTAAATATGAAATGCCTTATAATTTTTTTTCTTATGAAATATCTTGTTTATAGATGATAAGGTTCTTCATGAGCTCAAATTTGGTGTTAAGAGGGTTGAAAATACAAAATAGTCCTCAATTCCATGTGAAATTTGATGGTTGTCAAGGAGTATTAATTGATGAATTGTCCATTTCTGCACCAAAACTTAGTCCCAATACTGATGGAATCCACTTAGGAAACACAAGGGATGTTGGAATATATAATTCCTTGATAAGCAATGGTAAATAATAAAGATCATTGTCACACTTTCTCAATATAGAATTTTTATTCTATGGTTTTTTTATTTAATTATGTCACATAATGTTACATTTTATTTAATAGGTGATGATTGCATTTCAATTGGTCCTGGATGTTCAGATGTGAATGTGGATGGTGTTACTTGTGCTCCTACCCACGGAATTAGGTATGTCACATAGTTTCTGTTATTTTAGTTAAATTGGGTTGTTAACTATCAATTAATTATGATTAATTTAGGAATAAAATAAAAATAAGCTACAAACTTAGTTGATGGTATTCATATAAATATATACACCCAAATTCTGTGGTCCAAAAATCATTTCAAAAGGCAAAATGAATGAAAAGTGACTTTAACCTGTAGTGTGAGTGTAATAACTCTTGAAGGTGTCTTATAAGATAGCAATTTATCTTCAAGAAAAATAAATAAATAATGGAAAATTTTTGTTTGAAATTGAAACCTTTTTAACATCAAATGATCACAGGGTTAATTTTCTTTTTTAGAGATTTATGTGACTATAGTACTTTAACAGTTGGAAGTTTCAAACTGCCAATCATTAATGTGACAATAGTTTTTGTTCTTCACTTCTGTAATTCTGTTTTGTCCCCTGCTCCTAGGTAGCAATCTATTACCTTGGGACTAATTTTTTGGACATGACTTTTAAAGATGTTGATTTTGTAAAACTTATTAACATAAAATTGAGTTTAAAGAAAACTGATTTATATTCATATGATTTTATTTTAAAATCACAGTTTATTATTTTAGAGGTATAAAACTGATTTTATATGTTTGGATGTTATAGAGTATAATTGATATTGTCTCAAAAATTAACTCTAACTTAAAGCTAGAATTTTGAGCTTCTAACTCTCCAATTGTTTTTAAGATTAAGTTTTTTTTTTCAATTCACTTTTACATAAATATATACAACAAAAAATAAATTGACATTCAACGCACTACTTATACCACAGTTGATGAGAAGTACACACTTAGTGGACTAAACATGTTATAACATCTACGAAAATGTCTGATACTAGAGACCAAAATAAACTTGAAAAGAAAAAAAAAAACTCTGAAAACATTTACAATCTTTCATAAAATAATTACATAATTGCAATCAGTGTCATAGATTAGAAGTTGTTTTATGTTGTATGGCTTGATTCCAATTATGTAATCATTTTATGAAAAATTGTAAATGTTCATTGAGTTATGAGAGTTCTTTTTTTTCTTCAAATTTATTTTGGTCTCTAGTATACATCTTCTTAGAATTATTTTGGTCTCTAGTATGAGACATCTTCCTAGAAGTTACATGTGATGTGCTATTTTGGAACCTATAGAATTTTGAGTAGCAAGCTTCTATGTAGCTATAATTAAAAACCTTACTGATGTGATTTCGACAGCATTGGAAGCCTTGGAGTACACAATTCCCACGCATGTGTCTCAAACCTAACAGTCCGCAACTCAGTCATAAAAGAATCAGACAATGGACTAAGAATCAAAACATGGCAAGGAGGTACAGGATCGGTAACAGGTTTAACATTTGACAACATCCAAATGGAGAATGTAAGAAACTGTATCAACATAGACCAATTCTACTGTTTATCAAAAGAGTGTATGAACCAAACATCAGCAGTGTATGTAAACAATATCTCCTACAGAAAAATAAAGGGTACATATGATGTGAGAACACCTCCAATACACTTTGCTTGTAGTGACACTGTTGCTTGCACAAACATCACACTCTCTGAGATTGAACTTTTACCTTATGAAGGTGAATTAGTTGATGACCCTTTTTGTTGGAATGCTTATGGAAGACAAGAGACTTTGACTATTCCTCCACTTGATTGTTTAAGGGAAGGTGAGCCTGAAACGGTTGTGGAGTTATCTGAATATGAATGCAATAATTAA
Protein sequence

>MS.gene67862.t1

MILFIHNVEGRYHHKKPKKTSPAPSDPSPPSSNPPTSPSVPSDPYPYPNNPGESPSDCVFDVRSFGAVGDGNADDTAAFRAAWKAACAVDSGVLLAPENYCFKITSTIFSGPCKPGLVFQIDGTLMAPDGPNCWPEADSKSQWLVFYRLDQMTLNGTGIIEGNGEQWWDLPCKPHRSPNGKTVSGPCVSPTMIRFFMSSNLVLRGLKIQNSPQFHVKFDGCQGVLIDELSISAPKLSPNTDGIHLGNTRDVGIYNSLISNGDDCISIGPGCSDVNVDGVTCAPTHGISIGSLGVHNSHACVSNLTVRNSVIKESDNGLRIKTWQGGTGSVTGLTFDNIQMENVRNCINIDQFYCLSKECMNQTSAVYVNNISYRKIKGTYDVRTPPIHFACSDTVACTNITLSEIELLPYEGELVDDPFCWNAYGRQETLTIPPLDCLREGEPETVVELSEYECNN