AlfalfaGEDB | MS.gene68103

M. sativa cultivar XinJiangDaYe / MS.gene68103

KEGG: K11252
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0032991 GO:0032993 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:1901363
PFAM: PF00125.27

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68103.t1	XP_013469298.1	99.3	137	1	0	1	137	1	137	3.10E-41	177.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68103.t1	Q1S9I9	98.9	91	1	0	47	137	58	148	1.8e-42	173.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68103.t1	I3S6Z4	99.3	137	1	0	1	137	1	137	2.2e-41	177.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04972	MS.gene68103	0.809904	1.50E-50	-1.69E-46
MS.gene04985	MS.gene68103	0.829379	5.32E-55	-1.69E-46
MS.gene051263	MS.gene68103	0.803152	4.00E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68103.t1	MTR_1g492910	99.270	137	1	0	1	137	1	137	1.25e-94	269
MS.gene68103.t1	MTR_1g090780	98.540	137	2	0	1	137	1	137	8.98e-94	267
MS.gene68103.t1	MTR_4g063240	85.714	147	11	3	1	137	1	147	4.57e-80	233
MS.gene68103.t1	MTR_4g063220	86.486	148	9	3	1	137	1	148	2.51e-76	223
MS.gene68103.t1	MTR_4g063990	86.486	148	9	3	1	137	1	148	2.51e-76	223
MS.gene68103.t1	MTR_4g063200	86.486	148	9	3	1	137	1	148	2.51e-76	223
MS.gene68103.t1	MTR_4g064020	86.486	148	9	3	1	137	1	148	2.51e-76	223
MS.gene68103.t1	MTR_4g071180	86.486	148	9	3	1	137	1	148	2.17e-75	221
MS.gene68103.t1	MTR_4g070240	86.486	148	9	3	1	137	1	148	2.17e-75	221
MS.gene68103.t1	MTR_4g088025	88.811	143	10	3	1	137	1	143	4.38e-75	220
MS.gene68103.t1	MTR_4g070245	85.135	148	11	3	1	137	1	148	7.18e-74	217
MS.gene68103.t1	MTR_4g063275	83.784	148	13	3	1	137	1	148	4.48e-73	215
MS.gene68103.t1	MTR_4g064983	85.417	144	10	3	1	133	1	144	1.17e-71	214
MS.gene68103.t1	MTR_7g099960	94.495	109	5	1	29	137	31	138	1.20e-63	191
MS.gene68103.t1	MTR_1g068600	92.661	109	6	1	29	137	30	136	1.70e-62	187
MS.gene68103.t1	MTR_2g084480	62.921	89	33	0	48	136	136	224	3.66e-38	129
MS.gene68103.t1	MTR_8g069990	69.663	89	24	1	8	96	3	88	7.21e-37	121
MS.gene68103.t1	MTR_4g063270	90.196	51	5	0	75	125	1	51	5.41e-27	95.5
MS.gene68103.t1	MTR_7g114580	87.805	41	5	0	97	137	11	51	2.51e-19	75.9
MS.gene68103.t1	MTR_4g032777	36.364	99	58	1	28	121	12	110	4.10e-13	62.4
MS.gene68103.t1	MTR_2g083800	67.273	55	15	2	8	61	11	63	1.64e-11	56.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68103.t1	AT2G37470	87.681	138	15	2	1	136	1	138	3.41e-74	218
MS.gene68103.t1	AT3G45980	82.667	150	13	3	1	137	1	150	2.06e-73	216
MS.gene68103.t1	AT2G28720	81.457	151	14	2	1	137	1	151	3.23e-73	215
MS.gene68103.t1	AT3G53650	85.612	139	17	3	1	137	1	138	9.67e-71	209
MS.gene68103.t1	AT1G07790	82.667	150	11	4	1	137	1	148	1.36e-70	209
MS.gene68103.t1	AT5G59910	86.567	134	16	2	6	137	17	150	4.89e-69	205
MS.gene68103.t1	AT3G46030	88.372	129	13	2	11	137	17	145	1.60e-68	203
MS.gene68103.t1	AT5G22880	79.577	142	15	2	10	137	4	145	1.12e-65	196
MS.gene68103.t1	AT5G02570	83.453	139	14	2	1	137	1	132	1.97e-65	195
MS.gene68103.t1	AT3G09480	78.832	137	18	2	1	137	1	126	1.29e-61	185
MS.gene68103.t1	AT1G08170	56.989	93	40	0	42	134	143	235	7.17e-38	129

Find 34 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATTCAAGAGCTAGTGAATT+TGG	0.219657	1.1:-66324283	None:intergenic
GCTGTTCGTCTTGTTCTTCC+TGG	0.277831	1.1:+66324216	MS.gene68103:CDS
CTTGAAGATGTAGATCTTGT+AGG	0.279657	1.1:-66324034	None:intergenic
TCGGTTGAGGATGCTTCCTT+TGG	0.289986	1.1:-66323976	None:intergenic
GTTTCTCTTCTTCTTCTTGT+CGG	0.325136	1.1:-66323995	None:intergenic
GCAGGTTTCTTCTCTCCCTT+TGG	0.338176	1.1:-66323895	None:intergenic
TGGGATCTTCTTCTCCGCTT+TGG	0.356795	1.1:-66323956	None:intergenic
CTTCTTCTTCTTGTCGGTTG+AGG	0.378677	1.1:-66323989	None:intergenic
CGGTTGAGGATGCTTCCTTT+GGG	0.395504	1.1:-66323975	None:intergenic
TCTGCTGGTGCCTTCTCTGC+GGG	0.397953	1.1:-66323928	None:intergenic
CTTGTTGTAGCGAGCAAGTC+TGG	0.401541	1.1:-66324160	None:intergenic
AGTGAATTTGGTGACAGCTT+TGG	0.407583	1.1:-66324271	None:intergenic
TGGTATCTCAAGCAAGGCTA+TGG	0.409396	1.1:+66324083	MS.gene68103:CDS
TCTGCGGGCTTCTTCTCAGC+AGG	0.422937	1.1:-66323913	None:intergenic
CTACAAGATCTACATCTTCA+AGG	0.431521	1.1:+66324035	MS.gene68103:CDS
GGTGACAGCTTTGGCTCCCT+CGG	0.469008	1.1:-66324262	None:intergenic
CATCTTCAAGGTTCTCAAGC+AGG	0.470454	1.1:+66324047	MS.gene68103:CDS
TCAATCAACAATGGCACCAA+AGG	0.515027	1.1:+66323879	None:intergenic
GGTATCTCAAGCAAGGCTAT+GGG	0.517327	1.1:+66324084	MS.gene68103:CDS
GCGTGCTTAGCGAGTTCACC+AGG	0.523823	1.1:-66324234	None:intergenic
CTCTGCTGGTGCCTTCTCTG+CGG	0.531286	1.1:-66323929	None:intergenic
TTGCTTGAGATACCAATGTC+AGG	0.545226	1.1:-66324075	None:intergenic
TGAGAAGAAGCCCGCAGAGA+AGG	0.546802	1.1:+66323918	MS.gene68103:CDS
CAATCAACAATGGCACCAAA+GGG	0.556792	1.1:+66323880	None:intergenic
CTGAATCTCACGAGAAGTGA+TGG	0.558881	1.1:-66324190	None:intergenic
TATCTTTGAGAAGCTAGCTC+AGG	0.563267	1.1:+66324131	MS.gene68103:CDS
CGCTAAGCACGCTGTCTCCG+AGG	0.575955	1.1:+66324245	MS.gene68103:CDS
GCTAAGCACGCTGTCTCCGA+GGG	0.579952	1.1:+66324246	MS.gene68103:CDS
AAGCAGGTTCATCCTGACAT+TGG	0.605433	1.1:+66324063	MS.gene68103:CDS
TGACATTGGTATCTCAAGCA+AGG	0.620719	1.1:+66324077	MS.gene68103:CDS
GTATCTCAAGCAAGGCTATG+GGG	0.662407	1.1:+66324085	MS.gene68103:CDS
ACCAGCAGAGAAAACCAAAG+CGG	0.665379	1.1:+66323942	MS.gene68103:CDS
AGCGGAGAAGAAGATCCCAA+AGG	0.675483	1.1:+66323960	MS.gene68103:CDS
ATCTCACGAGAAGTGATGGT+AGG	0.676602	1.1:-66324186	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	CTACAAGATCTACATCTTCA+AGG	+	chr1.1:66324035-66324054	MS.gene68103:CDS	35.0%
	CTTGAAGATGTAGATCTTGT+AGG	-	chr1.1:66324037-66324056	None:intergenic	35.0%
	GTTTCTCTTCTTCTTCTTGT+CGG	-	chr1.1:66323998-66324017	None:intergenic	35.0%
	TGACATTGGTATCTCAAGCA+AGG	+	chr1.1:66324077-66324096	MS.gene68103:CDS	40.0%
	TTGCTTGAGATACCAATGTC+AGG	-	chr1.1:66324078-66324097	None:intergenic	40.0%
!	AGTGAATTTGGTGACAGCTT+TGG	-	chr1.1:66324274-66324293	None:intergenic	40.0%
!	TATCTTTGAGAAGCTAGCTC+AGG	+	chr1.1:66324131-66324150	MS.gene68103:CDS	40.0%
	AAGCAGGTTCATCCTGACAT+TGG	+	chr1.1:66324063-66324082	MS.gene68103:CDS	45.0%
	ACCAGCAGAGAAAACCAAAG+CGG	+	chr1.1:66323942-66323961	MS.gene68103:CDS	45.0%
	ATCTCACGAGAAGTGATGGT+AGG	-	chr1.1:66324189-66324208	None:intergenic	45.0%
	CATCTTCAAGGTTCTCAAGC+AGG	+	chr1.1:66324047-66324066	MS.gene68103:CDS	45.0%
	CTGAATCTCACGAGAAGTGA+TGG	-	chr1.1:66324193-66324212	None:intergenic	45.0%
	GGTATCTCAAGCAAGGCTAT+GGG	+	chr1.1:66324084-66324103	MS.gene68103:CDS	45.0%
	GTATCTCAAGCAAGGCTATG+GGG	+	chr1.1:66324085-66324104	MS.gene68103:CDS	45.0%
	TGGTATCTCAAGCAAGGCTA+TGG	+	chr1.1:66324083-66324102	MS.gene68103:CDS	45.0%
!	CTTCTTCTTCTTGTCGGTTG+AGG	-	chr1.1:66323992-66324011	None:intergenic	45.0%
	AGCGGAGAAGAAGATCCCAA+AGG	+	chr1.1:66323960-66323979	MS.gene68103:CDS	50.0%
	CGGTTGAGGATGCTTCCTTT+GGG	-	chr1.1:66323978-66323997	None:intergenic	50.0%
	GCAGGTTTCTTCTCTCCCTT+TGG	-	chr1.1:66323898-66323917	None:intergenic	50.0%
	TCGGTTGAGGATGCTTCCTT+TGG	-	chr1.1:66323979-66323998	None:intergenic	50.0%
	TGGGATCTTCTTCTCCGCTT+TGG	-	chr1.1:66323959-66323978	None:intergenic	50.0%
!	CTTGTTGTAGCGAGCAAGTC+TGG	-	chr1.1:66324163-66324182	None:intergenic	50.0%
!	GCTGTTCGTCTTGTTCTTCC+TGG	+	chr1.1:66324216-66324235	MS.gene68103:CDS	50.0%
!!!	TCCGCTTTGGTTTTCTCTGC+TGG	-	chr1.1:66323946-66323965	None:intergenic	50.0%
	TGAGAAGAAGCCCGCAGAGA+AGG	+	chr1.1:66323918-66323937	MS.gene68103:CDS	55.0%
	CTCTGCTGGTGCCTTCTCTG+CGG	-	chr1.1:66323932-66323951	None:intergenic	60.0%
	GCGTGCTTAGCGAGTTCACC+AGG	-	chr1.1:66324237-66324256	None:intergenic	60.0%
	GCTAAGCACGCTGTCTCCGA+GGG	+	chr1.1:66324246-66324265	MS.gene68103:CDS	60.0%
	TCTGCGGGCTTCTTCTCAGC+AGG	-	chr1.1:66323916-66323935	None:intergenic	60.0%
	TCTGCTGGTGCCTTCTCTGC+GGG	-	chr1.1:66323931-66323950	None:intergenic	60.0%
!	GGTGACAGCTTTGGCTCCCT+CGG	-	chr1.1:66324265-66324284	None:intergenic	60.0%
	CGCTAAGCACGCTGTCTCCG+AGG	+	chr1.1:66324245-66324264	MS.gene68103:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	66323889	66324302	66323889	ID=MS.gene68103
chr1.1	mRNA	66323889	66324302	66323889	ID=MS.gene68103.t1;Parent=MS.gene68103
chr1.1	exon	66323889	66324302	66323889	ID=MS.gene68103.t1.exon1;Parent=MS.gene68103.t1
chr1.1	CDS	66323889	66324302	66323889	ID=cds.MS.gene68103.t1;Parent=MS.gene68103.t1

Gene Sequence

>MS.gene68103

ATGGCACCAAAGGGAGAGAAGAAACCTGCTGAGAAGAAGCCCGCAGAGAAGGCACCAGCAGAGAAAACCAAAGCGGAGAAGAAGATCCCAAAGGAAGCATCCTCAACCGACAAGAAGAAGAAGAGAAACAAGAAGAGCGTTGAAACCTACAAGATCTACATCTTCAAGGTTCTCAAGCAGGTTCATCCTGACATTGGTATCTCAAGCAAGGCTATGGGGATCATGAACAGCTTCATCAATGATATCTTTGAGAAGCTAGCTCAGGAATCTTCCAGACTTGCTCGCTACAACAAGAAACCTACCATCACTTCTCGTGAGATTCAGACTGCTGTTCGTCTTGTTCTTCCTGGTGAACTCGCTAAGCACGCTGTCTCCGAGGGAGCCAAAGCTGTCACCAAATTCACTAGCTCTTGA

Protein sequence

>MS.gene68103.t1

MAPKGEKKPAEKKPAEKAPAEKTKAEKKIPKEASSTDKKKKRNKKSVETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSREIQTAVRLVLPGELAKHAVSEGAKAVTKFTSS