AlfalfaGEDB | MS.gene68214

M. sativa cultivar XinJiangDaYe / MS.gene68214

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515 GO:0046983
PFAM: PF00010.29

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68214.t1	XP_013462329.1	93.4	257	10	1	4	260	137	386	8.00E-131	476.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68214.t1	Q9CAA9	52.0	196	70	6	59	253	297	469	1.7e-41	171.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68214.t1	A0A072V302	93.4	257	10	1	4	260	137	386	5.8e-131	476.5

TFs/TRs:

Gene ID	Type	Classification
MS.gene68214.t1	TF	bHLH

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049758	MS.gene68214	0.803689	3.09E-49	-1.69E-46
MS.gene051471	MS.gene68214	0.804912	1.72E-49	-1.69E-46
MS.gene052326	MS.gene68214	0.801182	1.02E-48	-1.69E-46
MS.gene055422	MS.gene68214	0.800343	1.51E-48	-1.69E-46
MS.gene055428	MS.gene68214	0.811092	8.32E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68214.t1	MTR_3g116770	93.385	257	10	1	4	260	137	386	0.0	504
MS.gene68214.t1	MTR_3g116770	92.996	257	10	2	4	260	137	385	6.33e-179	498
MS.gene68214.t1	MTR_4g070320	66.540	263	62	11	4	255	82	329	3.11e-109	319
MS.gene68214.t1	MTR_5g048860	48.523	237	95	8	31	257	324	543	3.95e-57	191
MS.gene68214.t1	MTR_8g065740	58.065	155	53	2	10	152	238	392	1.44e-51	174
MS.gene68214.t1	MTR_7g053410	45.703	256	118	7	10	257	265	507	2.52e-51	175
MS.gene68214.t1	MTR_7g053410	45.703	256	118	7	10	257	265	507	2.87e-51	175
MS.gene68214.t1	MTR_1g017350	44.589	231	91	6	15	245	215	408	1.11e-50	171
MS.gene68214.t1	MTR_1g017350	44.589	231	91	5	15	245	215	408	1.13e-50	171
MS.gene68214.t1	MTR_1g017350	45.022	231	87	6	15	245	215	405	1.80e-50	171
MS.gene68214.t1	MTR_1g017350	45.022	231	87	5	15	245	215	405	1.82e-50	171
MS.gene68214.t1	MTR_8g012290	44.649	271	121	11	7	259	226	485	8.82e-49	168
MS.gene68214.t1	MTR_8g012290	43.911	271	122	11	7	259	226	484	4.97e-48	166
MS.gene68214.t1	MTR_8g062240	70.755	106	24	2	62	166	148	247	4.20e-43	148
MS.gene68214.t1	MTR_5g037250	58.273	139	41	3	15	153	73	194	4.41e-43	146
MS.gene68214.t1	MTR_3g498825	80.899	89	17	0	49	137	131	219	6.77e-43	149
MS.gene68214.t1	MTR_7g092510	61.017	118	38	1	41	158	157	266	1.33e-42	148
MS.gene68214.t1	MTR_8g099880	82.558	86	14	1	60	144	140	225	4.19e-42	146
MS.gene68214.t1	MTR_1g059270	67.010	97	32	0	58	154	153	249	1.42e-41	143
MS.gene68214.t1	MTR_1g059270	65.347	101	35	0	58	158	153	253	1.70e-41	145
MS.gene68214.t1	MTR_6g084120	60.227	88	24	2	61	146	151	229	7.35e-26	102
MS.gene68214.t1	MTR_4g131160	72.727	66	17	1	65	130	196	260	2.01e-23	98.2
MS.gene68214.t1	MTR_4g131160	72.727	66	17	1	65	130	199	263	2.06e-23	98.2
MS.gene68214.t1	MTR_1g080890	69.697	66	19	1	65	130	193	257	3.24e-22	94.4
MS.gene68214.t1	MTR_2g098310	55.224	67	30	0	63	129	330	396	1.78e-16	78.6
MS.gene68214.t1	MTR_4g065870	58.462	65	27	0	65	129	355	419	2.21e-16	78.6
MS.gene68214.t1	MTR_2g098310	56.061	66	29	0	63	128	330	395	5.09e-16	77.4
MS.gene68214.t1	MTR_1g069155	52.174	69	32	1	67	135	319	386	6.23e-16	77.4
MS.gene68214.t1	MTR_4g033295	55.385	65	29	0	65	129	118	182	9.14e-16	73.9
MS.gene68214.t1	MTR_1g084980	33.600	125	76	3	18	139	415	535	1.10e-15	76.6
MS.gene68214.t1	MTR_4g065870	58.065	62	26	0	65	126	355	416	1.40e-15	76.3
MS.gene68214.t1	MTR_1g052470	53.623	69	32	0	65	133	298	366	1.98e-15	75.5
MS.gene68214.t1	MTR_7g092540	50.000	74	37	0	65	138	243	316	2.10e-15	74.7
MS.gene68214.t1	MTR_4g033295	53.731	67	30	1	65	130	118	184	2.26e-15	72.4
MS.gene68214.t1	MTR_1g070870	65.152	66	22	1	65	130	120	184	5.66e-15	73.2
MS.gene68214.t1	MTR_1g070870	65.152	66	22	1	65	130	123	187	6.02e-15	73.2
MS.gene68214.t1	MTR_7g092540	53.125	64	30	0	65	128	243	306	1.88e-14	72.0
MS.gene68214.t1	MTR_7g099540	50.000	68	33	1	68	135	255	321	3.67e-14	72.0
MS.gene68214.t1	MTR_3g028520	46.753	77	41	0	53	129	310	386	8.44e-14	70.9
MS.gene68214.t1	MTR_3g027650	46.753	77	41	0	53	129	310	386	8.44e-14	70.9
MS.gene68214.t1	MTR_3g027650	47.297	74	39	0	53	126	310	383	4.25e-13	68.6
MS.gene68214.t1	MTR_8g036235	49.231	65	33	0	65	129	316	380	5.28e-13	68.6
MS.gene68214.t1	MTR_1g019240	47.692	65	33	1	67	131	87	150	5.52e-13	67.4
MS.gene68214.t1	MTR_4g107320	47.368	76	38	2	83	156	60	135	6.99e-13	64.7
MS.gene68214.t1	MTR_1g018090	44.828	87	45	2	43	129	254	337	1.47e-12	67.0
MS.gene68214.t1	MTR_3g449770	50.000	64	31	1	67	130	311	373	2.03e-12	67.0
MS.gene68214.t1	MTR_7g099540	49.206	63	31	1	68	130	255	316	2.99e-12	66.2
MS.gene68214.t1	MTR_7g111320	39.806	103	57	3	32	130	395	496	4.73e-12	65.9
MS.gene68214.t1	MTR_3g103030	37.719	114	67	2	16	129	183	292	1.07e-11	64.3
MS.gene68214.t1	MTR_7g110810	36.111	108	59	3	76	183	213	310	1.34e-11	64.3
MS.gene68214.t1	MTR_1g116790	47.059	68	36	0	65	132	265	332	1.42e-11	63.9
MS.gene68214.t1	MTR_5g017040	53.571	56	25	1	76	131	116	170	1.55e-11	63.9
MS.gene68214.t1	MTR_5g005110	50.000	64	31	1	66	129	225	287	2.02e-11	63.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68214.t1	AT4G34530	47.984	248	85	8	7	240	113	330	1.10e-58	190
MS.gene68214.t1	AT1G68920	47.162	229	97	6	26	253	263	468	2.51e-55	185
MS.gene68214.t1	AT1G68920	47.162	229	97	6	26	253	264	469	2.59e-55	185
MS.gene68214.t1	AT1G68920	47.162	229	97	6	26	253	264	469	2.59e-55	185
MS.gene68214.t1	AT1G68920	47.162	229	97	6	26	253	264	469	2.59e-55	185
MS.gene68214.t1	AT3G07340	53.403	191	78	4	61	248	254	436	3.94e-53	179
MS.gene68214.t1	AT2G18300	47.917	240	78	11	4	241	133	327	1.24e-52	174
MS.gene68214.t1	AT3G07340	52.632	190	81	3	61	247	254	437	1.88e-52	176
MS.gene68214.t1	AT2G18300	62.838	148	44	5	4	150	133	270	7.33e-52	172
MS.gene68214.t1	AT2G18300	62.838	148	44	5	4	150	133	270	1.32e-51	172
MS.gene68214.t1	AT1G10120	47.442	215	67	6	30	244	178	346	1.98e-50	169
MS.gene68214.t1	AT1G10120	47.442	215	67	6	30	244	178	346	1.98e-50	169
MS.gene68214.t1	AT4G36540	58.170	153	43	4	3	154	98	230	5.16e-50	166
MS.gene68214.t1	AT4G36540	59.060	149	40	4	3	150	98	226	2.75e-49	164
MS.gene68214.t1	AT3G23690	61.268	142	50	3	13	152	140	278	3.49e-49	166
MS.gene68214.t1	AT5G48560	64.444	135	45	3	47	180	284	416	2.03e-48	166
MS.gene68214.t1	AT5G48560	64.444	135	45	3	47	180	284	416	4.12e-48	166
MS.gene68214.t1	AT1G26260	59.459	148	41	3	10	152	126	259	1.00e-47	161
MS.gene68214.t1	AT1G26260	59.459	148	41	3	10	152	155	288	2.99e-47	161
MS.gene68214.t1	AT1G26260	59.459	148	41	3	10	152	177	310	4.53e-47	161
MS.gene68214.t1	AT1G26260	59.459	148	41	3	10	152	177	310	4.53e-47	161
MS.gene68214.t1	AT1G26260	59.459	148	41	3	10	152	177	310	4.53e-47	161
MS.gene68214.t1	AT1G26260	59.459	148	41	3	10	152	177	310	4.53e-47	161
MS.gene68214.t1	AT1G59640	52.222	180	62	5	4	181	79	236	2.76e-45	153
MS.gene68214.t1	AT5G50915	61.832	131	43	3	19	144	88	216	3.31e-45	153
MS.gene68214.t1	AT5G50915	61.832	131	43	3	19	144	88	216	3.31e-45	153
MS.gene68214.t1	AT5G50915	61.832	131	43	3	19	144	88	216	1.10e-44	152
MS.gene68214.t1	AT5G50915	61.832	131	43	3	19	144	88	216	1.10e-44	152
MS.gene68214.t1	AT1G59640	55.000	160	64	2	4	161	79	232	4.16e-44	152
MS.gene68214.t1	AT3G57800	55.634	142	55	2	58	192	197	337	1.22e-43	152
MS.gene68214.t1	AT2G42300	70.968	93	27	0	59	151	179	271	2.79e-43	148
MS.gene68214.t1	AT2G42300	70.652	92	27	0	59	150	179	270	9.43e-42	145
MS.gene68214.t1	AT5G62610	81.579	76	14	0	61	136	149	224	1.58e-38	136
MS.gene68214.t1	AT5G62610	81.579	76	14	0	61	136	149	224	1.58e-38	136
MS.gene68214.t1	AT1G18400	75.000	80	20	0	63	142	143	222	3.48e-36	129
MS.gene68214.t1	AT1G73830	50.000	142	60	1	1	142	94	224	3.67e-36	129
MS.gene68214.t1	AT1G73830	50.000	142	60	1	1	142	94	224	4.32e-36	129
MS.gene68214.t1	AT1G18400	75.000	80	20	0	63	142	143	222	7.48e-36	129
MS.gene68214.t1	AT1G25330	65.217	92	32	0	61	152	100	191	6.41e-35	125
MS.gene68214.t1	AT3G57800	41.799	189	55	3	58	192	197	384	1.28e-34	129
MS.gene68214.t1	AT5G58010	65.000	80	23	2	65	140	99	177	2.16e-24	99.4
MS.gene68214.t1	AT2G24260	71.212	66	18	1	65	130	35	99	7.75e-24	97.1
MS.gene68214.t1	AT4G30980	72.727	66	17	1	65	130	130	194	1.35e-23	97.4
MS.gene68214.t1	AT4G30980	72.727	66	17	1	65	130	178	242	2.30e-23	97.8
MS.gene68214.t1	AT2G24260	71.212	66	18	1	65	130	138	202	4.46e-23	96.7
MS.gene68214.t1	AT2G42300	76.087	46	11	0	59	104	179	224	7.18e-19	83.2
MS.gene68214.t1	AT2G42300	76.087	46	11	0	59	104	179	224	7.18e-19	83.2
MS.gene68214.t1	AT2G42300	76.087	46	11	0	59	104	179	224	9.81e-19	82.8
MS.gene68214.t1	AT2G20180	42.553	94	48	2	37	130	184	271	1.73e-16	78.6
MS.gene68214.t1	AT2G20180	42.553	94	48	2	37	130	184	271	1.73e-16	78.6
MS.gene68214.t1	AT2G20180	42.553	94	48	2	37	130	184	271	1.73e-16	78.6
MS.gene68214.t1	AT2G42280	37.405	131	58	4	9	128	227	344	1.74e-16	78.6
MS.gene68214.t1	AT2G20180	42.553	94	48	2	37	130	255	342	1.92e-16	79.0
MS.gene68214.t1	AT2G20180	42.553	94	48	2	37	130	255	342	1.92e-16	79.0
MS.gene68214.t1	AT2G20180	42.553	94	48	2	37	130	255	342	1.92e-16	79.0
MS.gene68214.t1	AT2G20180	42.553	94	48	2	37	130	255	342	1.92e-16	79.0
MS.gene68214.t1	AT2G42280	36.434	129	60	3	9	128	227	342	1.96e-16	78.2
MS.gene68214.t1	AT1G09530	37.719	114	66	3	18	130	292	401	1.16e-15	76.6
MS.gene68214.t1	AT1G09530	37.719	114	66	3	18	130	292	401	1.16e-15	76.6
MS.gene68214.t1	AT1G09530	37.719	114	66	3	18	130	292	401	1.16e-15	76.6
MS.gene68214.t1	AT1G09530	37.719	114	66	3	18	130	292	401	1.16e-15	76.6
MS.gene68214.t1	AT1G09530	37.719	114	66	3	18	130	292	401	1.16e-15	76.6
MS.gene68214.t1	AT1G09530	37.719	114	66	3	18	130	292	401	1.16e-15	76.6
MS.gene68214.t1	AT1G51140	43.269	104	52	3	29	128	267	367	3.01e-15	75.1
MS.gene68214.t1	AT4G28790	30.811	185	83	7	20	200	242	385	3.23e-15	75.1
MS.gene68214.t1	AT1G03040	50.549	91	44	1	40	130	114	203	6.02e-15	73.6
MS.gene68214.t1	AT4G28800	33.803	142	69	3	68	209	252	368	7.02e-15	74.3
MS.gene68214.t1	AT4G09180	50.746	67	33	0	63	129	182	248	8.79e-15	72.4
MS.gene68214.t1	AT2G20180	41.758	91	47	2	37	127	255	339	9.26e-15	73.2
MS.gene68214.t1	AT1G35460	52.308	65	31	0	65	129	181	245	1.23e-14	72.0
MS.gene68214.t1	AT1G03040	60.274	73	28	1	58	130	137	208	1.46e-14	72.4
MS.gene68214.t1	AT4G02590	63.636	66	23	1	65	130	83	147	2.32e-14	71.2
MS.gene68214.t1	AT1G03040	60.274	73	28	1	58	130	137	208	2.43e-14	72.0
MS.gene68214.t1	AT4G28815	56.897	58	24	1	68	125	71	127	2.43e-14	70.9
MS.gene68214.t1	AT4G02590	63.636	66	23	1	65	130	146	210	3.00e-14	71.6
MS.gene68214.t1	AT4G02590	63.636	66	23	1	65	130	146	210	3.00e-14	71.6
MS.gene68214.t1	AT4G28815	56.897	58	24	1	68	125	147	203	7.43e-14	70.5
MS.gene68214.t1	AT1G27740	36.134	119	71	2	11	129	117	230	1.36e-13	68.9
MS.gene68214.t1	AT4G28800	52.459	61	28	1	68	128	264	323	7.85e-13	68.2
MS.gene68214.t1	AT2G46970	29.016	193	92	8	55	224	187	357	9.43e-13	67.8
MS.gene68214.t1	AT2G46970	29.016	193	92	8	55	224	178	348	1.05e-12	67.4
MS.gene68214.t1	AT2G46970	29.016	193	92	8	55	224	209	379	1.18e-12	67.4
MS.gene68214.t1	AT4G33880	41.935	93	51	2	39	129	238	329	1.32e-12	67.0
MS.gene68214.t1	AT4G28790	35.398	113	56	2	20	132	242	337	1.88e-12	66.6
MS.gene68214.t1	AT4G00050	50.769	65	31	1	65	129	105	168	3.49e-12	65.5
MS.gene68214.t1	AT4G00050	50.769	65	31	1	65	129	207	270	5.72e-12	65.5
MS.gene68214.t1	AT4G00050	50.769	65	31	1	65	129	207	270	5.77e-12	65.5
MS.gene68214.t1	AT2G43140	50.000	64	32	0	65	128	134	197	6.36e-12	63.5
MS.gene68214.t1	AT2G43140	50.000	64	32	0	65	128	231	294	7.59e-12	64.7
MS.gene68214.t1	AT4G36930	50.820	61	29	1	71	131	197	256	8.07e-12	65.1
MS.gene68214.t1	AT1G05805	46.875	64	34	0	65	128	283	346	2.19e-11	63.5
MS.gene68214.t1	AT2G14760	40.404	99	51	3	37	129	204	300	3.48e-11	62.8
MS.gene68214.t1	AT3G59060	34.188	117	70	3	68	183	253	363	3.57e-11	63.2
MS.gene68214.t1	AT3G59060	34.188	117	70	3	68	183	253	363	3.57e-11	63.2
MS.gene68214.t1	AT3G59060	34.188	117	70	3	68	183	253	363	3.57e-11	63.2
MS.gene68214.t1	AT3G59060	34.188	117	70	3	68	183	253	363	3.59e-11	63.2
MS.gene68214.t1	AT2G43010	39.583	96	50	2	40	128	219	313	5.39e-11	62.8
MS.gene68214.t1	AT2G43010	39.583	96	50	2	40	128	219	313	5.51e-11	62.8
MS.gene68214.t1	AT2G43010	39.583	96	50	2	40	128	226	320	5.67e-11	62.4
MS.gene68214.t1	AT2G43010	39.583	96	50	2	40	128	261	355	5.99e-11	62.4
MS.gene68214.t1	AT2G43010	39.583	96	50	2	40	128	219	313	6.90e-11	62.4

Find 49 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCACCTTCCCATGTTGTATT+AGG	0.244216	3.1:-92151435	None:intergenic
CCACAGCAATTTGTTCCTTA+TGG	0.249421	3.1:+92151196	MS.gene68214:CDS
AATTGAAGTTGTATAGGTTT+TGG	0.253509	3.1:-92151461	None:intergenic
TCTCAATCTCAGTTATTTAC+AGG	0.262036	3.1:+92151513	MS.gene68214:CDS
TGGAATGAACCCTACTGATA+TGG	0.311726	3.1:+92151231	MS.gene68214:CDS
AAAGTTTGAGGAATGGATAT+AGG	0.336253	3.1:-92151277	None:intergenic
GATCAAAATTGAAGTTGTAT+AGG	0.339897	3.1:-92151467	None:intergenic
ATTGGGATTCCCGGTGATCT+TGG	0.342453	3.1:-92150383	None:intergenic
AAGTTTGAGGAATGGATATA+GGG	0.345771	3.1:-92151276	None:intergenic
CCAGGATGCAACAAAATTAC+AGG	0.386637	3.1:+92150706	MS.gene68214:CDS
AAAGACAAGAGGATCAAAAT+AGG	0.388939	3.1:+92150343	MS.gene68214:CDS
TATGTATGATGCAGGTTAGA+AGG	0.402202	3.1:+92150641	MS.gene68214:intron
TTGCTTGTTGTTTAGGCTGT+TGG	0.409201	3.1:+92150307	None:intergenic
AGCTATGGCTGTCGGTGGCT+TGG	0.431984	3.1:-92150531	None:intergenic
AAATGCAAACTTTCAAGCTA+TGG	0.441658	3.1:+92151123	MS.gene68214:CDS
TGCTCGAACATGAATGTAAT+CGG	0.461397	3.1:-92150500	None:intergenic
GAAGAATCAATAAAAGTTTG+AGG	0.465715	3.1:-92151289	None:intergenic
ATCAATAAAAGTTTGAGGAA+TGG	0.483827	3.1:-92151284	None:intergenic
CTTCTAAGTCCCATATCAGT+AGG	0.485186	3.1:-92151241	None:intergenic
AACAAAATTACAGGAAAAGC+TGG	0.485342	3.1:+92150715	MS.gene68214:CDS
ATGTATGATGCAGGTTAGAA+GGG	0.501649	3.1:+92150642	MS.gene68214:intron
GGAATGAACCCTACTGATAT+GGG	0.502117	3.1:+92151232	MS.gene68214:CDS
TTTGCATTTGCATCACAAGT+AGG	0.509857	3.1:-92151109	None:intergenic
TCAATATTGAAATCAAGCCT+TGG	0.519925	3.1:-92150935	None:intergenic
CCAGCATCTAATCCACCATA+AGG	0.520595	3.1:-92151211	None:intergenic
AATGCAAACTTTCAAGCTAT+GGG	0.521255	3.1:+92151124	MS.gene68214:CDS
CAGCAATTTGTTCCTTATGG+TGG	0.522613	3.1:+92151199	MS.gene68214:CDS
AGAAGATACTTCAAATTCAA+AGG	0.529575	3.1:+92150447	MS.gene68214:CDS
TTACTCTTTCAGCTAAGCTA+TGG	0.530789	3.1:-92150546	None:intergenic
TTCAGCTAAGCTATGGCTGT+CGG	0.556309	3.1:-92150539	None:intergenic
CCTTATGGTGGATTAGATGC+TGG	0.561668	3.1:+92151211	MS.gene68214:CDS
CTACTGATATGGGACTTAGA+AGG	0.561670	3.1:+92151242	MS.gene68214:CDS
TATCTGCAAGATTTAGTACC+AGG	0.562856	3.1:+92150688	MS.gene68214:CDS
GACGGCGAATCCAAGATCAC+CGG	0.574848	3.1:+92150373	MS.gene68214:CDS
TTCTAAGTCCCATATCAGTA+GGG	0.581586	3.1:-92151240	None:intergenic
GTAAATAACTGAGATTGAGA+AGG	0.584711	3.1:-92151510	None:intergenic
GGTGAATTGAACTGAAGATA+AGG	0.590017	3.1:-92151175	None:intergenic
CTACCTAATACAACATGGGA+AGG	0.593979	3.1:+92151432	MS.gene68214:CDS
AGCTAAGCTATGGCTGTCGG+TGG	0.605688	3.1:-92150536	None:intergenic
AATTAGCTACTGTTAATCCA+AGG	0.613412	3.1:+92150918	MS.gene68214:CDS
AAATTCTACCTAATACAACA+TGG	0.624191	3.1:+92151427	MS.gene68214:CDS
ACGGCGAATCCAAGATCACC+GGG	0.628691	3.1:+92150374	MS.gene68214:CDS
ATCAAAATAGGTGCTGAGGA+CGG	0.629527	3.1:+92150355	MS.gene68214:CDS
GAGGATCAAAATAGGTGCTG+AGG	0.635802	3.1:+92150351	MS.gene68214:CDS
AGTTTGAGGAATGGATATAG+GGG	0.638076	3.1:-92151275	None:intergenic
AAGTTGAGAACAAAGACAAG+AGG	0.638388	3.1:+92150332	MS.gene68214:CDS
ATTCATGTTCGAGCACGTCG+TGG	0.642577	3.1:+92150508	MS.gene68214:CDS
AATTCTACCTAATACAACAT+GGG	0.654432	3.1:+92151428	MS.gene68214:CDS
CCATAAGGAACAAATTGCTG+TGG	0.664587	3.1:-92151196	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAAGTAGTAATAATATACTT+TGG	+	chr3.1:92150566-92150585	MS.gene68214:intron	15.0%
!!!	TAACATAAACAAATTTGTTA+TGG	-	chr3.1:92150872-92150891	None:intergenic	15.0%
!!	AAAATTAAAACACTAGTAAC+GGG	-	chr3.1:92151356-92151375	None:intergenic	20.0%
!!	ATTAAGCTAAAATACAGATT+AGG	-	chr3.1:92151019-92151038	None:intergenic	20.0%
!!	CAAAATTAAAACACTAGTAA+CGG	-	chr3.1:92151357-92151376	None:intergenic	20.0%
!!	TAATTATATATGTATGATGC+AGG	+	chr3.1:92150633-92150652	MS.gene68214:intron	20.0%
!!!	ATAAACAAATTTGTTATGGA+TGG	-	chr3.1:92150868-92150887	None:intergenic	20.0%
!!!	GTGTTTTAATTTTGTTGTAT+TGG	+	chr3.1:92151363-92151382	MS.gene68214:intron	20.0%
!	AAAAATATTAACAGAGAGAG+TGG	-	chr3.1:92151331-92151350	None:intergenic	25.0%
!	AAATTCTACCTAATACAACA+TGG	+	chr3.1:92151427-92151446	MS.gene68214:CDS	25.0%
!	AATTCTACCTAATACAACAT+GGG	+	chr3.1:92151428-92151447	MS.gene68214:CDS	25.0%
!	AGAAGATACTTCAAATTCAA+AGG	+	chr3.1:92150447-92150466	MS.gene68214:CDS	25.0%
!	ATCAATAAAAGTTTGAGGAA+TGG	-	chr3.1:92151287-92151306	None:intergenic	25.0%
!	GAAGAATCAATAAAAGTTTG+AGG	-	chr3.1:92151292-92151311	None:intergenic	25.0%
!	GATCAAAATTGAAGTTGTAT+AGG	-	chr3.1:92151470-92151489	None:intergenic	25.0%
!	TGATGATCTATTTGAAAAAG+AGG	+	chr3.1:92150955-92150974	MS.gene68214:CDS	25.0%
!!	AATTGAAGTTGTATAGGTTT+TGG	-	chr3.1:92151464-92151483	None:intergenic	25.0%
!!!	TTGTTGTTCTTTTTGGTATT+GGG	-	chr3.1:92150403-92150422	None:intergenic	25.0%
	AAAGACAAGAGGATCAAAAT+AGG	+	chr3.1:92150343-92150362	MS.gene68214:CDS	30.0%
	AAAGTTTGAGGAATGGATAT+AGG	-	chr3.1:92151280-92151299	None:intergenic	30.0%
	AAATGCAAACTTTCAAGCTA+TGG	+	chr3.1:92151123-92151142	MS.gene68214:CDS	30.0%
	AACAAAATTACAGGAAAAGC+TGG	+	chr3.1:92150715-92150734	MS.gene68214:CDS	30.0%
	AAGTTTGAGGAATGGATATA+GGG	-	chr3.1:92151279-92151298	None:intergenic	30.0%
	AATGCAAACTTTCAAGCTAT+GGG	+	chr3.1:92151124-92151143	MS.gene68214:CDS	30.0%
	AATTAGCTACTGTTAATCCA+AGG	+	chr3.1:92150918-92150937	MS.gene68214:CDS	30.0%
	CATAGATAGATCAATGTGAT+AGG	-	chr3.1:92150843-92150862	None:intergenic	30.0%
	GGTAATAAAGAATATGCAGA+TGG	+	chr3.1:92150976-92150995	MS.gene68214:intron	30.0%
	GTAAATAACTGAGATTGAGA+AGG	-	chr3.1:92151513-92151532	None:intergenic	30.0%
	TCAATATTGAAATCAAGCCT+TGG	-	chr3.1:92150938-92150957	None:intergenic	30.0%
	TCTCAATCTCAGTTATTTAC+AGG	+	chr3.1:92151513-92151532	MS.gene68214:CDS	30.0%
!!	GTTGTTGTTCTTTTTGGTAT+TGG	-	chr3.1:92150404-92150423	None:intergenic	30.0%
!!	TTTGTGTTGATTCCTTACAT+AGG	+	chr3.1:92151081-92151100	MS.gene68214:intron	30.0%
	AAGTAGGAAACACCTATGTA+AGG	-	chr3.1:92151096-92151115	None:intergenic	35.0%
	AAGTTGAGAACAAAGACAAG+AGG	+	chr3.1:92150332-92150351	MS.gene68214:CDS	35.0%
	AGTTTGAGGAATGGATATAG+GGG	-	chr3.1:92151278-92151297	None:intergenic	35.0%
	ATGTATGATGCAGGTTAGAA+GGG	+	chr3.1:92150642-92150661	MS.gene68214:intron	35.0%
	GGTGAATTGAACTGAAGATA+AGG	-	chr3.1:92151178-92151197	None:intergenic	35.0%
	GTCTCTTCAAAAACAAGTTG+AGG	+	chr3.1:92150768-92150787	MS.gene68214:CDS	35.0%
	TATCTGCAAGATTTAGTACC+AGG	+	chr3.1:92150688-92150707	MS.gene68214:CDS	35.0%
	TATGTATGATGCAGGTTAGA+AGG	+	chr3.1:92150641-92150660	MS.gene68214:intron	35.0%
	TGCTCGAACATGAATGTAAT+CGG	-	chr3.1:92150503-92150522	None:intergenic	35.0%
	TTACTCTTTCAGCTAAGCTA+TGG	-	chr3.1:92150549-92150568	None:intergenic	35.0%
	TTCTAAGTCCCATATCAGTA+GGG	-	chr3.1:92151243-92151262	None:intergenic	35.0%
	TTTGCATTTGCATCACAAGT+AGG	-	chr3.1:92151112-92151131	None:intergenic	35.0%
!	GATTCTTCTTGTTTCTCTGT+AGG	+	chr3.1:92151304-92151323	MS.gene68214:intron	35.0%
!	TGCTACACAGTTTTCTTTGT+TGG	-	chr3.1:92150428-92150447	None:intergenic	35.0%
!!!	GTTGGTGTTGTTGTTCTTTT+TGG	-	chr3.1:92150410-92150429	None:intergenic	35.0%
	CCAGGATGCAACAAAATTAC+AGG	+	chr3.1:92150706-92150725	MS.gene68214:CDS	40.0%
	CCATAAGGAACAAATTGCTG+TGG	-	chr3.1:92151199-92151218	None:intergenic	40.0%
	CTACCTAATACAACATGGGA+AGG	+	chr3.1:92151432-92151451	MS.gene68214:CDS	40.0%
	CTACTGATATGGGACTTAGA+AGG	+	chr3.1:92151242-92151261	MS.gene68214:CDS	40.0%
	CTTCTAAGTCCCATATCAGT+AGG	-	chr3.1:92151244-92151263	None:intergenic	40.0%
	GGAATGAACCCTACTGATAT+GGG	+	chr3.1:92151232-92151251	MS.gene68214:CDS	40.0%
	TCACCTTCCCATGTTGTATT+AGG	-	chr3.1:92151438-92151457	None:intergenic	40.0%
	TGGAATGAACCCTACTGATA+TGG	+	chr3.1:92151231-92151250	MS.gene68214:CDS	40.0%
!	CAGCAATTTGTTCCTTATGG+TGG	+	chr3.1:92151199-92151218	MS.gene68214:CDS	40.0%
!	CCACAGCAATTTGTTCCTTA+TGG	+	chr3.1:92151196-92151215	MS.gene68214:CDS	40.0%
!	CCTGTAATTTTGTTGCATCC+TGG	-	chr3.1:92150709-92150728	None:intergenic	40.0%
!!	ATCAAAATAGGTGCTGAGGA+CGG	+	chr3.1:92150355-92150374	MS.gene68214:CDS	40.0%
!!	CTTTTTGGTATTGGGATTCC+CGG	-	chr3.1:92150395-92150414	None:intergenic	40.0%
	CCAGCATCTAATCCACCATA+AGG	-	chr3.1:92151214-92151233	None:intergenic	45.0%
	TTCAGCTAAGCTATGGCTGT+CGG	-	chr3.1:92150542-92150561	None:intergenic	45.0%
!	GAGGATCAAAATAGGTGCTG+AGG	+	chr3.1:92150351-92150370	MS.gene68214:CDS	45.0%
!!	CCTTATGGTGGATTAGATGC+TGG	+	chr3.1:92151211-92151230	MS.gene68214:CDS	45.0%
	ATTCATGTTCGAGCACGTCG+TGG	+	chr3.1:92150508-92150527	MS.gene68214:CDS	50.0%
	ATTGGGATTCCCGGTGATCT+TGG	-	chr3.1:92150386-92150405	None:intergenic	50.0%
	ACGGCGAATCCAAGATCACC+GGG	+	chr3.1:92150374-92150393	MS.gene68214:CDS	55.0%
	AGCTAAGCTATGGCTGTCGG+TGG	-	chr3.1:92150539-92150558	None:intergenic	55.0%
	GACGGCGAATCCAAGATCAC+CGG	+	chr3.1:92150373-92150392	MS.gene68214:CDS	55.0%
	AGCTATGGCTGTCGGTGGCT+TGG	-	chr3.1:92150534-92150553	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	92150322	92151534	92150322	ID=MS.gene68214
chr3.1	mRNA	92150322	92151534	92150322	ID=MS.gene68214.t1;Parent=MS.gene68214
chr3.1	exon	92150322	92150564	92150322	ID=MS.gene68214.t1.exon1;Parent=MS.gene68214.t1
chr3.1	CDS	92150322	92150564	92150322	ID=cds.MS.gene68214.t1;Parent=MS.gene68214.t1
chr3.1	exon	92150655	92150789	92150655	ID=MS.gene68214.t1.exon2;Parent=MS.gene68214.t1
chr3.1	CDS	92150655	92150789	92150655	ID=cds.MS.gene68214.t1;Parent=MS.gene68214.t1
chr3.1	exon	92150905	92150976	92150905	ID=MS.gene68214.t1.exon3;Parent=MS.gene68214.t1
chr3.1	CDS	92150905	92150976	92150905	ID=cds.MS.gene68214.t1;Parent=MS.gene68214.t1
chr3.1	exon	92151103	92151321	92151103	ID=MS.gene68214.t1.exon4;Parent=MS.gene68214.t1
chr3.1	CDS	92151103	92151321	92151103	ID=cds.MS.gene68214.t1;Parent=MS.gene68214.t1
chr3.1	exon	92151423	92151534	92151423	ID=MS.gene68214.t1.exon5;Parent=MS.gene68214.t1
chr3.1	CDS	92151423	92151534	92151423	ID=cds.MS.gene68214.t1;Parent=MS.gene68214.t1

Gene Sequence

>MS.gene68214

GCTGTTGGTGAAGTTGAGAACAAAGACAAGAGGATCAAAATAGGTGCTGAGGACGGCGAATCCAAGATCACCGGGAATCCCAATACCAAAAAGAACAACAACACCAACAAAGAAAACTGTGTAGCAGAAGATACTTCAAATTCAAAGGAAAATTCAAAAGTTTCTGATGTTCAAAAGACCGATTACATTCATGTTCGAGCACGTCGTGGCCAAGCCACCGACAGCCATAGCTTAGCTGAAAGAGTAAGTAGTAATAATATACTTTGGTTTCTGTCTGACTGAGAAAAGTAAAATCAAAGTATCTGAATAATTAATTATATATGTATGATGCAGGTTAGAAGGGAAAAGATTAGTGAGAGAATGAAGTATCTGCAAGATTTAGTACCAGGATGCAACAAAATTACAGGAAAAGCTGGAATGCTTGATGAAATCATCAACTATGTTCAGTCTCTTCAAAAACAAGTTGAGGTAATTAAAAGAGTTAATTAATTAAGTTCATTACATTGATTCTTAATTTGCCTATCACATTGATCTATCTATGATCCATCCATAACAAATTTGTTTATGTTAATTTTCTTTGCAGTTCTTGTCAATGAAATTAGCTACTGTTAATCCAAGGCTTGATTTCAATATTGATGATCTATTTGAAAAAGAGGTAATAAAGAATATGCAGATGGAAGAATTTCACTTTTCACCTAATCTGTATTTTAGCTTAATTTGAAATCTGTAATATAATATTAATATTAATATATTCTTCTATTTGTGTTGATTCCTTACATAGGTGTTTCCTACTTGTGATGCAAATGCAAACTTTCAAGCTATGGGAATGTCAACAGAATTGAATAGTAACAATCCTTATCTTCAGTTCAATTCACCACAGCAATTTGTTCCTTATGGTGGATTAGATGCTGGAATGAACCCTACTGATATGGGACTTAGAAGGAGCATAAGTGCCCCTATATCCATTCCTCAAACTTTTATTGATTCTTCTTGTTTCTCTGTAGGTCCACTCTCTCTGTTAATATTTTTGTCCCGTTACTAGTGTTTTAATTTTGTTGTATTGGTTTATTTTATTAATTTTATCTTTGTTTATGTGTGTAGCAGCAAATTCTACCTAATACAACATGGGAAGGTGATTTCCAAAACCTATACAACTTCAATTTTGATCAAGCAAGAGCAACATCTTTTCCTTCTCAATCTCAGTTATTTACAG

Protein sequence

>MS.gene68214.t1

AVGEVENKDKRIKIGAEDGESKITGNPNTKKNNNTNKENCVAEDTSNSKENSKVSDVQKTDYIHVRARRGQATDSHSLAERVRREKISERMKYLQDLVPGCNKITGKAGMLDEIINYVQSLQKQVEFLSMKLATVNPRLDFNIDDLFEKEVFPTCDANANFQAMGMSTELNSNNPYLQFNSPQQFVPYGGLDAGMNPTDMGLRRSISAPISIPQTFIDSSCFSQQILPNTTWEGDFQNLYNFNFDQARATSFPSQSQLFT