AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene68440

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68440.t1 MTR_1g090657 94.975 398 17 2 1 396 1 397 0.0 757
MS.gene68440.t1 MTR_1g090657 91.709 398 14 3 1 396 1 381 0.0 722
MS.gene68440.t1 MTR_4g057530 39.175 97 56 1 298 394 206 299 5.39e-13 69.7
MS.gene68440.t1 MTR_4g057530 29.614 233 146 6 39 257 13 241 4.93e-12 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68440.t1 AT5G04320 27.027 259 160 5 78 330 49 284 1.55e-11 66.2
MS.gene68440.t1 AT5G04320 27.027 259 160 5 78 330 49 284 1.55e-11 66.2
MS.gene68440.t1 AT5G04320 27.027 259 160 5 78 330 49 284 1.55e-11 66.2
MS.gene68440.t1 AT5G04320 26.820 261 162 5 76 330 23 260 1.74e-11 65.9
MS.gene68440.t1 AT3G44960 29.187 209 126 6 47 249 15 207 3.45e-11 63.9

Find 83 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATTGGATACTTCAAATTT+TGG 0.152385 1.1:-66137028 None:intergenic
TGACATATCATCACATAAAT+TGG 0.184226 1.1:-66137044 None:intergenic
TTCAGCTGGTTCTGGTTTCT+CGG 0.284010 1.1:-66136984 None:intergenic
CTGGTTTCTCGGGTCTGAAC+AGG 0.293350 1.1:-66136973 None:intergenic
CTCGCATCCTCTTGAAAGTT+TGG 0.293934 1.1:+66136608 MS.gene68440:CDS
GGTGATTCTGTTTCTGTTGT+TGG 0.311273 1.1:+66135164 MS.gene68440:CDS
AAGAAGTCTTCAGCTGGTTC+TGG 0.317331 1.1:-66136992 None:intergenic
GTGCTTTGGTTGGGCCTTGT+TGG 0.319039 1.1:-66135219 None:intergenic
CAACCTCCCGTCGCTGAAAC+TGG 0.327729 1.1:+66134802 MS.gene68440:CDS
GTGCAGGGTGGTGCTTTGGT+TGG 0.330722 1.1:-66135229 None:intergenic
GAGAAAGAATCTACATCATT+AGG 0.338286 1.1:+66136636 MS.gene68440:CDS
AAAACAAAGGAGAAGCAAAA+AGG 0.343577 1.1:+66136236 MS.gene68440:CDS
TGCAGGGTGGTGCTTTGGTT+GGG 0.346409 1.1:-66135228 None:intergenic
AGGCTAATTACATTGAGATT+TGG 0.379116 1.1:-66136269 None:intergenic
GCGTTGAAAGCTGGAAAATC+AGG 0.392933 1.1:+66136549 MS.gene68440:CDS
AGCTGCTCAGAAGAAAATTC+TGG 0.404402 1.1:+66135187 MS.gene68440:CDS
TCCGGCCACATCGTGAACTT+TGG 0.440039 1.1:-66136661 None:intergenic
GGTGGAGGAAGTGGTAGTGC+AGG 0.444862 1.1:-66135245 None:intergenic
TCAGCTGGTTCTGGTTTCTC+GGG 0.444940 1.1:-66136983 None:intergenic
CTGTGAAGGTTGTTTCGTAC+AGG 0.446552 1.1:+66137173 MS.gene68440:CDS
GGTAGTGCAGGGTGGTGCTT+TGG 0.446807 1.1:-66135233 None:intergenic
AAAGGAATGTTGAAAATAAG+AGG 0.462622 1.1:+66136811 MS.gene68440:CDS
GAAATGATGAGGAAGCTCTT+AGG 0.464073 1.1:+66135411 MS.gene68440:CDS
CAGCTGATCGACGCGAAGGC+CGG 0.476480 1.1:-66137153 None:intergenic
AAATCTGCAGCGTTGAAAGC+TGG 0.478212 1.1:+66136540 MS.gene68440:CDS
AAGATCGGAGTCGAGGAAGA+TGG 0.483738 1.1:-66134779 None:intergenic
TAAACAAGTCAAATCAGAAA+AGG 0.486455 1.1:+66136377 MS.gene68440:CDS
TCAGTTGAAGGTGGAGGAAG+TGG 0.491527 1.1:-66135254 None:intergenic
CAGAAAAGGTTGACAGTCAA+AGG 0.498447 1.1:+66136391 MS.gene68440:CDS
ATTACATTCAGTATCTTGTC+CGG 0.500303 1.1:-66136679 None:intergenic
TATAATATACCTTGAGAATG+AGG 0.502497 1.1:-66135289 None:intergenic
TGCCTGTACACTTGATCCTC+AGG 0.504464 1.1:+66137108 MS.gene68440:CDS
CCTTGTCCAGTTTCAGCGAC+GGG 0.512611 1.1:-66134808 None:intergenic
AGAACTTGTTCAGTGTTAGC+AGG 0.515952 1.1:-66136783 None:intergenic
GATTAGTGCAAGCAGACAAA+TGG 0.517988 1.1:+66135761 MS.gene68440:CDS
TTGACTTGTTTAACAACAGC+TGG 0.518722 1.1:-66136366 None:intergenic
AGCTATGAAATTAACATCGA+TGG 0.525803 1.1:+66135923 MS.gene68440:CDS
CAATTAATCTGAAAATGCGT+AGG 0.528526 1.1:+66137203 MS.gene68440:CDS
GTAACACGAAGATCGTCTAT+TGG 0.529512 1.1:+66137130 MS.gene68440:CDS
CTGAACAAGTTCTTGCTGAA+AGG 0.532133 1.1:+66136793 MS.gene68440:CDS
TTCGCGTCGATCAGCTGTGA+AGG 0.535513 1.1:+66137159 MS.gene68440:CDS
GTGGAGGAAGTGGTAGTGCA+GGG 0.536378 1.1:-66135244 None:intergenic
AGATCGGAGTCGAGGAAGAT+GGG 0.546317 1.1:-66134778 None:intergenic
TCCAAAGTTCACGATGTGGC+CGG 0.550716 1.1:+66136660 MS.gene68440:CDS
TGGTTTCTCGGGTCTGAACA+GGG 0.557557 1.1:-66136972 None:intergenic
TCATTTGCCAAACTTTCAAG+AGG 0.559353 1.1:-66136615 None:intergenic
ATCTGAAAATGCGTAGGGAT+AGG 0.559401 1.1:+66137209 MS.gene68440:CDS
CACGAAGATCGTCTATTGGC+CGG 0.560924 1.1:+66137134 MS.gene68440:CDS
AATTAATCTGAAAATGCGTA+GGG 0.561635 1.1:+66137204 MS.gene68440:CDS
GTATCAAAGAAGTCTTCAGC+TGG 0.569283 1.1:-66136998 None:intergenic
TGTGAAGGTTGTTTCGTACA+GGG 0.572533 1.1:+66137174 MS.gene68440:CDS
ATTACAGGCATAGTTTGAGA+AGG 0.573967 1.1:+66136942 MS.gene68440:intron
TTCAGTGTTAGCAGGTCCTG+AGG 0.575831 1.1:-66136775 None:intergenic
CTCCGTTGACCTCATTCTCA+AGG 0.579673 1.1:+66135280 MS.gene68440:CDS
TCAACGGAGACATCAGTTGA+AGG 0.580490 1.1:-66135266 None:intergenic
TACCTGAGGATCAAGTGTAC+AGG 0.580964 1.1:-66137110 None:intergenic
CTCAGGAAAATGAAATGATG+AGG 0.584643 1.1:+66135400 MS.gene68440:intron
CAAACAGGTGTTCTTCGAGA+AGG 0.585970 1.1:+66136517 MS.gene68440:intron
CCATGGCATTGTTGCAGATG+AGG 0.589331 1.1:+66136069 MS.gene68440:CDS
GAGGAAGTGGTAGTGCAGGG+TGG 0.592603 1.1:-66135241 None:intergenic
CGAATCGCCACAACAATGGA+AGG 0.596720 1.1:+66134751 None:intergenic
TGAAGACTTCTTTGATACAG+AGG 0.602950 1.1:+66137003 MS.gene68440:CDS
GAAGTTTCGCTTGATGCTGT+AGG 0.604493 1.1:-66137076 None:intergenic
ACCTTGTCCAGTTTCAGCGA+CGG 0.605270 1.1:-66134809 None:intergenic
CCTCATCTGCAACAATGCCA+TGG 0.605304 1.1:-66136069 None:intergenic
TTCACAGCTGATCGACGCGA+AGG 0.610564 1.1:-66137157 None:intergenic
AACAGAGCAAAACTGATCCA+TGG 0.616879 1.1:+66136052 MS.gene68440:CDS
AAAGTTTGAAGAAGAAAACA+AGG 0.617125 1.1:+66136204 MS.gene68440:CDS
GAGACATCAGTTGAAGGTGG+AGG 0.623469 1.1:-66135260 None:intergenic
AAGGGAGAAGAACAAAACAA+AGG 0.623499 1.1:+66136223 MS.gene68440:CDS
GAGGTTGAAGATCGGAGTCG+AGG 0.623839 1.1:-66134786 None:intergenic
TGCTAATACTCAAATGACGC+GGG 0.629072 1.1:+66135629 MS.gene68440:CDS
AGCGACGGGAGGTTGAAGAT+CGG 0.630621 1.1:-66134794 None:intergenic
TGTCCAGTTTCAGCGACGGG+AGG 0.630744 1.1:-66134805 None:intergenic
CAAACTTTCAAGAGGATGCG+AGG 0.631072 1.1:-66136607 None:intergenic
GACGATCTTCGTGTTACCTG+AGG 0.646396 1.1:-66137124 None:intergenic
TACCTTGAGAATGAGGTCAA+CGG 0.647194 1.1:-66135282 None:intergenic
ACGGAGACATCAGTTGAAGG+TGG 0.656398 1.1:-66135263 None:intergenic
TTGCTAATACTCAAATGACG+CGG 0.657610 1.1:+66135628 MS.gene68440:CDS
CCCGTCGCTGAAACTGGACA+AGG 0.671344 1.1:+66134808 MS.gene68440:CDS
AGGATCCAAAGTTCACGATG+TGG 0.700483 1.1:+66136656 MS.gene68440:CDS
TCTGGCTGATATAACCAACA+AGG 0.712159 1.1:+66135205 MS.gene68440:CDS
AAGTTTGAAGAAGAAAACAA+GGG 0.730503 1.1:+66136205 MS.gene68440:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATATATATTTTCCTTTAT+TGG + chr1.1:66136099-66136118 MS.gene68440:intron 10.0%
!! GGAAAAAAAAAAAAGATAAA+TGG - chr1.1:66136711-66136730 None:intergenic 15.0%
!! CGTATAGTTAATTGAAAAAT+GGG + chr1.1:66135093-66135112 MS.gene68440:intron 20.0%
!! TCGTATAGTTAATTGAAAAA+TGG + chr1.1:66135092-66135111 MS.gene68440:intron 20.0%
!!! AAAAAGAGGTAAAATTTTGA+AGG + chr1.1:66135142-66135161 MS.gene68440:CDS 20.0%
!!! AAAAGAGGTAAAATTTTGAA+GGG + chr1.1:66135143-66135162 MS.gene68440:CDS 20.0%
!!! AAAGCAATTTTCAGAAATTT+CGG + chr1.1:66135583-66135602 MS.gene68440:CDS 20.0%
!!! AAATTGGATACTTCAAATTT+TGG - chr1.1:66137031-66137050 None:intergenic 20.0%
!!! ATAGATAAACCTTTCAATTT+TGG - chr1.1:66135041-66135060 None:intergenic 20.0%
!!! ATTTTTTGAATTTGTTTCTC+AGG + chr1.1:66135383-66135402 MS.gene68440:intron 20.0%
!!! TACATTGATTGAAGATAAAA+AGG - chr1.1:66135483-66135502 None:intergenic 20.0%
!!! TTCAAAATTTTACCTCTTTT+TGG - chr1.1:66135143-66135162 None:intergenic 20.0%
!!! TTGATATTCCAATCTAAAAT+TGG + chr1.1:66135713-66135732 MS.gene68440:intron 20.0%
!!! TTTTTATCTTCAATCAATGT+AGG + chr1.1:66135482-66135501 MS.gene68440:intron 20.0%
! AAAGGAATGTTGAAAATAAG+AGG + chr1.1:66136811-66136830 MS.gene68440:CDS 25.0%
! AAAGTTTGAAGAAGAAAACA+AGG + chr1.1:66136204-66136223 MS.gene68440:CDS 25.0%
! AAGTTTGAAGAAGAAAACAA+GGG + chr1.1:66136205-66136224 MS.gene68440:CDS 25.0%
! AATTAATCTGAAAATGCGTA+GGG + chr1.1:66137204-66137223 MS.gene68440:CDS 25.0%
! CAAGCAAAAAATTATGTTGA+AGG - chr1.1:66136752-66136771 None:intergenic 25.0%
! CTATATATATGCAGACATTT+AGG + chr1.1:66135057-66135076 MS.gene68440:intron 25.0%
! TAAACAAGTCAAATCAGAAA+AGG + chr1.1:66136377-66136396 MS.gene68440:CDS 25.0%
! TATAATATACCTTGAGAATG+AGG - chr1.1:66135292-66135311 None:intergenic 25.0%
! TGACATATCATCACATAAAT+TGG - chr1.1:66137047-66137066 None:intergenic 25.0%
!! AAAGATCACCAATTTTAGAT+TGG - chr1.1:66135724-66135743 None:intergenic 25.0%
!! ATTAAATTTTGTAGACAGAG+TGG - chr1.1:66136302-66136321 None:intergenic 25.0%
!! TTAAATTTTGTAGACAGAGT+GGG - chr1.1:66136301-66136320 None:intergenic 25.0%
!!! GGAATAGTTTTTGTTTACTT+TGG + chr1.1:66135503-66135522 MS.gene68440:intron 25.0%
!!! TTAATGTCGAATTCTAAAGT+TGG + chr1.1:66136444-66136463 MS.gene68440:intron 25.0%
AAAACAAAGGAGAAGCAAAA+AGG + chr1.1:66136236-66136255 MS.gene68440:CDS 30.0%
AAACCATCAAAAACTAAAGC+AGG - chr1.1:66136003-66136022 None:intergenic 30.0%
AAATAAGAAATGAAAGCAGC+AGG - chr1.1:66135817-66135836 None:intergenic 30.0%
AACCATCAAAAACTAAAGCA+GGG - chr1.1:66136002-66136021 None:intergenic 30.0%
AGCTATGAAATTAACATCGA+TGG + chr1.1:66135923-66135942 MS.gene68440:CDS 30.0%
AGGAGAGAAACAAAAGAAAA+AGG - chr1.1:66136732-66136751 None:intergenic 30.0%
AGGCTAATTACATTGAGATT+TGG - chr1.1:66136272-66136291 None:intergenic 30.0%
AGTGAAATTCAGAATTGAAC+AGG - chr1.1:66134940-66134959 None:intergenic 30.0%
ATAGCTATTAGATCTAACTG+AGG + chr1.1:66135013-66135032 MS.gene68440:intron 30.0%
ATGATTATCACTACACTTCA+TGG + chr1.1:66135360-66135379 MS.gene68440:intron 30.0%
ATTACATTCAGTATCTTGTC+CGG - chr1.1:66136682-66136701 None:intergenic 30.0%
CAATTAATCTGAAAATGCGT+AGG + chr1.1:66137203-66137222 MS.gene68440:CDS 30.0%
CATTTACAAATTCATCAGGT+TGG - chr1.1:66135965-66135984 None:intergenic 30.0%
CGACATTAATCATGTGTAAA+AGG - chr1.1:66136433-66136452 None:intergenic 30.0%
GAGAAAGAATCTACATCATT+AGG + chr1.1:66136636-66136655 MS.gene68440:CDS 30.0%
TATGAATTCCACTTTCTGAA+TGG + chr1.1:66136846-66136865 MS.gene68440:intron 30.0%
TGCTCATTTACAAATTCATC+AGG - chr1.1:66135969-66135988 None:intergenic 30.0%
TGGATTCGAATGAATGAAAT+AGG + chr1.1:66136866-66136885 MS.gene68440:intron 30.0%
TGTCTTCACGAAAAAATTAC+AGG + chr1.1:66136927-66136946 MS.gene68440:intron 30.0%
! CTACGCATTTTCAGATTAAT+TGG - chr1.1:66137205-66137224 None:intergenic 30.0%
!!! GCAGACATTTAGGTTTTTTA+GGG + chr1.1:66135067-66135086 MS.gene68440:intron 30.0%
!!! TGCAGACATTTAGGTTTTTT+AGG + chr1.1:66135066-66135085 MS.gene68440:intron 30.0%
AAGGGAGAAGAACAAAACAA+AGG + chr1.1:66136223-66136242 MS.gene68440:CDS 35.0%
ACACCTGTTTGCATATTTGA+AGG - chr1.1:66136508-66136527 None:intergenic 35.0%
ATTACAGGCATAGTTTGAGA+AGG + chr1.1:66136942-66136961 MS.gene68440:intron 35.0%
ATTCGAATCCATTCAGAAAG+TGG - chr1.1:66136857-66136876 None:intergenic 35.0%
CTCAGGAAAATGAAATGATG+AGG + chr1.1:66135400-66135419 MS.gene68440:intron 35.0%
CTTGAACTAGCATGCAAAAA+TGG + chr1.1:66135891-66135910 MS.gene68440:CDS 35.0%
GAAATGAAAGAGAAGAAGAG+TGG - chr1.1:66134847-66134866 None:intergenic 35.0%
TCATTTGCCAAACTTTCAAG+AGG - chr1.1:66136618-66136637 None:intergenic 35.0%
TCTCCTTCAAATATGCAAAC+AGG + chr1.1:66136502-66136521 MS.gene68440:intron 35.0%
TGAAGACTTCTTTGATACAG+AGG + chr1.1:66137003-66137022 MS.gene68440:CDS 35.0%
TTGCTAATACTCAAATGACG+CGG + chr1.1:66135628-66135647 MS.gene68440:CDS 35.0%
! GATCTTTTTATCTGCAATGC+AGG + chr1.1:66135737-66135756 MS.gene68440:intron 35.0%
! GGTTTGTTCTGTTTATAGTG+TGG + chr1.1:66135524-66135543 MS.gene68440:intron 35.0%
! TGACTGCTTACTCTAAGAAA+AGG - chr1.1:66136179-66136198 None:intergenic 35.0%
! TTGATTAAGTGAAAGTGTGC+TGG - chr1.1:66136151-66136170 None:intergenic 35.0%
! TTTTCGTGAAGACATGAAAC+AGG - chr1.1:66136921-66136940 None:intergenic 35.0%
!! CATTCAATTTGTTGACATCC+AGG + chr1.1:66135854-66135873 MS.gene68440:intron 35.0%
!! GGTTTTGTCTTTTGATTCGT+AGG + chr1.1:66136018-66136037 MS.gene68440:intron 35.0%
!! TTGACTTGTTTAACAACAGC+TGG - chr1.1:66136369-66136388 None:intergenic 35.0%
!!! TTTTTGGTTTTTGACCTGCA+AGG - chr1.1:66135127-66135146 None:intergenic 35.0%
AACAGAGCAAAACTGATCCA+TGG + chr1.1:66136052-66136071 MS.gene68440:CDS 40.0%
AAGGAGAAGCAAAAAGGAAG+AGG + chr1.1:66136242-66136261 MS.gene68440:CDS 40.0%
ACTGAGGCTCCAAAATTGAA+AGG + chr1.1:66135029-66135048 MS.gene68440:intron 40.0%
AGCTGCTCAGAAGAAAATTC+TGG + chr1.1:66135187-66135206 MS.gene68440:CDS 40.0%
ATCTGAAAATGCGTAGGGAT+AGG + chr1.1:66137209-66137228 MS.gene68440:CDS 40.0%
CAAGCAGAAGAAGCCAATAA+AGG - chr1.1:66136115-66136134 None:intergenic 40.0%
CAGAAAAGGTTGACAGTCAA+AGG + chr1.1:66136391-66136410 MS.gene68440:CDS 40.0%
CTGAACAAGTTCTTGCTGAA+AGG + chr1.1:66136793-66136812 MS.gene68440:CDS 40.0%
GAAATGATGAGGAAGCTCTT+AGG + chr1.1:66135411-66135430 MS.gene68440:CDS 40.0%
GAACAGGAAAACCCTAATCT+CGG - chr1.1:66134924-66134943 None:intergenic 40.0%
GATTAGTGCAAGCAGACAAA+TGG + chr1.1:66135761-66135780 MS.gene68440:CDS 40.0%
GCAGGTCAAAAACCAAAAAG+AGG + chr1.1:66135128-66135147 MS.gene68440:intron 40.0%
GGTTATGGGAATGAAGGATT+GGG - chr1.1:66134869-66134888 None:intergenic 40.0%
GTAACACGAAGATCGTCTAT+TGG + chr1.1:66137130-66137149 MS.gene68440:CDS 40.0%
GTATCAAAGAAGTCTTCAGC+TGG - chr1.1:66137001-66137020 None:intergenic 40.0%
TACCTTGAGAATGAGGTCAA+CGG - chr1.1:66135285-66135304 None:intergenic 40.0%
TCTGGCTGATATAACCAACA+AGG + chr1.1:66135205-66135224 MS.gene68440:CDS 40.0%
TGCTAATACTCAAATGACGC+GGG + chr1.1:66135629-66135648 MS.gene68440:CDS 40.0%
TGGTGAGTCTTGAAAACCAA+TGG + chr1.1:66135781-66135800 MS.gene68440:intron 40.0%
! AATGGGTTTCATTTCCTTGC+AGG + chr1.1:66135110-66135129 MS.gene68440:intron 40.0%
! AGAACTTGTTCAGTGTTAGC+AGG - chr1.1:66136786-66136805 None:intergenic 40.0%
! ATTTGTTGACATCCAGGTGA+GGG + chr1.1:66135860-66135879 MS.gene68440:intron 40.0%
! TGTGAAGGTTGTTTCGTACA+GGG + chr1.1:66137174-66137193 MS.gene68440:CDS 40.0%
!! AATTTGTTGACATCCAGGTG+AGG + chr1.1:66135859-66135878 MS.gene68440:intron 40.0%
!! CGAATTCTAAAGTTGGACAC+CGG + chr1.1:66136451-66136470 MS.gene68440:intron 40.0%
!! GAATTCTAAAGTTGGACACC+GGG + chr1.1:66136452-66136471 MS.gene68440:intron 40.0%
!! GGTGATTCTGTTTCTGTTGT+TGG + chr1.1:66135164-66135183 MS.gene68440:CDS 40.0%
!! TATTGGCTTCTTCTGCTTGT+TGG + chr1.1:66136116-66136135 MS.gene68440:intron 40.0%
!! TTTTGTAGACAGAGTGGGTT+CGG - chr1.1:66136296-66136315 None:intergenic 40.0%
!!! AATTTTTTGCTTGCAGCCTC+AGG + chr1.1:66136759-66136778 MS.gene68440:intron 40.0%
!!! AGCCCTGCTTTAGTTTTTGA+TGG + chr1.1:66135997-66136016 MS.gene68440:intron 40.0%
AAAACCCTAATCTCGGAGTG+AGG - chr1.1:66134917-66134936 None:intergenic 45.0%
AAACCCTAATCTCGGAGTGA+GGG - chr1.1:66134916-66134935 None:intergenic 45.0%
AAGAGGGATGAGTAAACCCT+AGG - chr1.1:66134893-66134912 None:intergenic 45.0%
AGAGGGATGAGTAAACCCTA+GGG - chr1.1:66134892-66134911 None:intergenic 45.0%
AGGATCCAAAGTTCACGATG+TGG + chr1.1:66136656-66136675 MS.gene68440:CDS 45.0%
AGTAAACCCTAGGGGGTTAT+GGG - chr1.1:66134883-66134902 None:intergenic 45.0%
CAAACAGGTGTTCTTCGAGA+AGG + chr1.1:66136517-66136536 MS.gene68440:intron 45.0%
CAAACTTTCAAGAGGATGCG+AGG - chr1.1:66136610-66136629 None:intergenic 45.0%
CTCGCATCCTCTTGAAAGTT+TGG + chr1.1:66136608-66136627 MS.gene68440:CDS 45.0%
GAAGTTTCGCTTGATGCTGT+AGG - chr1.1:66137079-66137098 None:intergenic 45.0%
GCGTTGAAAGCTGGAAAATC+AGG + chr1.1:66136549-66136568 MS.gene68440:CDS 45.0%
GGGTTATGGGAATGAAGGAT+TGG - chr1.1:66134870-66134889 None:intergenic 45.0%
TACCTGAGGATCAAGTGTAC+AGG - chr1.1:66137113-66137132 None:intergenic 45.0%
TCAACGGAGACATCAGTTGA+AGG - chr1.1:66135269-66135288 None:intergenic 45.0%
TTCATTCCCATAACCCCCTA+GGG + chr1.1:66134874-66134893 MS.gene68440:intron 45.0%
! AAATCTGCAGCGTTGAAAGC+TGG + chr1.1:66136540-66136559 MS.gene68440:CDS 45.0%
! AAGAAGTCTTCAGCTGGTTC+TGG - chr1.1:66136995-66137014 None:intergenic 45.0%
! CTGTGAAGGTTGTTTCGTAC+AGG + chr1.1:66137173-66137192 MS.gene68440:CDS 45.0%
! GACAGTCAAAGGTATGAGCT+TGG + chr1.1:66136402-66136421 MS.gene68440:intron 45.0%
! TGCCTTGTTCCTAAGTATCC+CGG - chr1.1:66136473-66136492 None:intergenic 45.0%
!! TTCAGCTGGTTCTGGTTTCT+CGG - chr1.1:66136987-66137006 None:intergenic 45.0%
AAGATCGGAGTCGAGGAAGA+TGG - chr1.1:66134782-66134801 None:intergenic 50.0%
AATCTCGGAGTGAGGGAAGA+GGG - chr1.1:66134909-66134928 None:intergenic 50.0%
ACCTTGTCCAGTTTCAGCGA+CGG - chr1.1:66134812-66134831 None:intergenic 50.0%
ACGGAGACATCAGTTGAAGG+TGG - chr1.1:66135266-66135285 None:intergenic 50.0%
AGATCGGAGTCGAGGAAGAT+GGG - chr1.1:66134781-66134800 None:intergenic 50.0%
AGCAGGTGTCAATGCACCAT+TGG - chr1.1:66135800-66135819 None:intergenic 50.0%
AGGGATGAGTAAACCCTAGG+GGG - chr1.1:66134890-66134909 None:intergenic 50.0%
AGTTGGACACCGGGATACTT+AGG + chr1.1:66136461-66136480 MS.gene68440:intron 50.0%
CACCGGGATACTTAGGAACA+AGG + chr1.1:66136468-66136487 MS.gene68440:intron 50.0%
CACGAAGATCGTCTATTGGC+CGG + chr1.1:66137134-66137153 MS.gene68440:CDS 50.0%
CCATGGCATTGTTGCAGATG+AGG + chr1.1:66136069-66136088 MS.gene68440:CDS 50.0%
CCTCATCTGCAACAATGCCA+TGG - chr1.1:66136072-66136091 None:intergenic 50.0%
CTAGGGGGTTATGGGAATGA+AGG - chr1.1:66134875-66134894 None:intergenic 50.0%
CTCCGTTGACCTCATTCTCA+AGG + chr1.1:66135280-66135299 MS.gene68440:CDS 50.0%
CTTCATTCCCATAACCCCCT+AGG + chr1.1:66134873-66134892 MS.gene68440:intron 50.0%
CTTCCCTCACTCCGAGATTA+GGG + chr1.1:66134910-66134929 MS.gene68440:intron 50.0%
GACGATCTTCGTGTTACCTG+AGG - chr1.1:66137127-66137146 None:intergenic 50.0%
GAGACATCAGTTGAAGGTGG+AGG - chr1.1:66135263-66135282 None:intergenic 50.0%
GAGGGATGAGTAAACCCTAG+GGG - chr1.1:66134891-66134910 None:intergenic 50.0%
GAGTAAACCCTAGGGGGTTA+TGG - chr1.1:66134884-66134903 None:intergenic 50.0%
TAATCTCGGAGTGAGGGAAG+AGG - chr1.1:66134910-66134929 None:intergenic 50.0%
TCAGTTGAAGGTGGAGGAAG+TGG - chr1.1:66135257-66135276 None:intergenic 50.0%
TCCAAAGTTCACGATGTGGC+CGG + chr1.1:66136660-66136679 MS.gene68440:CDS 50.0%
TCTTCCCTCACTCCGAGATT+AGG + chr1.1:66134909-66134928 MS.gene68440:intron 50.0%
TGCCTGTACACTTGATCCTC+AGG + chr1.1:66137108-66137127 MS.gene68440:CDS 50.0%
TGGTTTCTCGGGTCTGAACA+GGG - chr1.1:66136975-66136994 None:intergenic 50.0%
TTCAGTGTTAGCAGGTCCTG+AGG - chr1.1:66136778-66136797 None:intergenic 50.0%
!! TCAGCTGGTTCTGGTTTCTC+GGG - chr1.1:66136986-66137005 None:intergenic 50.0%
AGCGACGGGAGGTTGAAGAT+CGG - chr1.1:66134797-66134816 None:intergenic 55.0%
CAAGCTGAAGTTCCCTCACC+TGG - chr1.1:66135875-66135894 None:intergenic 55.0%
CCTTGTCCAGTTTCAGCGAC+GGG - chr1.1:66134811-66134830 None:intergenic 55.0%
CTGGTTTCTCGGGTCTGAAC+AGG - chr1.1:66136976-66136995 None:intergenic 55.0%
GAGGTTGAAGATCGGAGTCG+AGG - chr1.1:66134789-66134808 None:intergenic 55.0%
TCCGGCCACATCGTGAACTT+TGG - chr1.1:66136664-66136683 None:intergenic 55.0%
TGCAGGGTGGTGCTTTGGTT+GGG - chr1.1:66135231-66135250 None:intergenic 55.0%
TTCACAGCTGATCGACGCGA+AGG - chr1.1:66137160-66137179 None:intergenic 55.0%
! GTGCTTTGGTTGGGCCTTGT+TGG - chr1.1:66135222-66135241 None:intergenic 55.0%
! GTGGAGGAAGTGGTAGTGCA+GGG - chr1.1:66135247-66135266 None:intergenic 55.0%
! TTCGCGTCGATCAGCTGTGA+AGG + chr1.1:66137159-66137178 MS.gene68440:CDS 55.0%
CAACCTCCCGTCGCTGAAAC+TGG + chr1.1:66134802-66134821 MS.gene68440:CDS 60.0%
CCCGTCGCTGAAACTGGACA+AGG + chr1.1:66134808-66134827 MS.gene68440:CDS 60.0%
GGTAGTGCAGGGTGGTGCTT+TGG - chr1.1:66135236-66135255 None:intergenic 60.0%
GTGCAGGGTGGTGCTTTGGT+TGG - chr1.1:66135232-66135251 None:intergenic 60.0%
TGTCCAGTTTCAGCGACGGG+AGG - chr1.1:66134808-66134827 None:intergenic 60.0%
! GAGGAAGTGGTAGTGCAGGG+TGG - chr1.1:66135244-66135263 None:intergenic 60.0%
! GGTGGAGGAAGTGGTAGTGC+AGG - chr1.1:66135248-66135267 None:intergenic 60.0%
! GTAGACAGAGTGGGTTCGGC+AGG - chr1.1:66136292-66136311 None:intergenic 60.0%
CAGCTGATCGACGCGAAGGC+CGG - chr1.1:66137156-66137175 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 66134766 66137234 66134766 ID=MS.gene68440
chr1.1 mRNA 66134766 66137234 66134766 ID=MS.gene68440.t1;Parent=MS.gene68440
chr1.1 exon 66134766 66134829 66134766 ID=MS.gene68440.t1.exon1;Parent=MS.gene68440.t1
chr1.1 CDS 66134766 66134829 66134766 ID=cds.MS.gene68440.t1;Parent=MS.gene68440.t1
chr1.1 exon 66135132 66135301 66135132 ID=MS.gene68440.t1.exon2;Parent=MS.gene68440.t1
chr1.1 CDS 66135132 66135301 66135132 ID=cds.MS.gene68440.t1;Parent=MS.gene68440.t1
chr1.1 exon 66135405 66135442 66135405 ID=MS.gene68440.t1.exon3;Parent=MS.gene68440.t1
chr1.1 CDS 66135405 66135442 66135405 ID=cds.MS.gene68440.t1;Parent=MS.gene68440.t1
chr1.1 exon 66135542 66135650 66135542 ID=MS.gene68440.t1.exon4;Parent=MS.gene68440.t1
chr1.1 CDS 66135542 66135650 66135542 ID=cds.MS.gene68440.t1;Parent=MS.gene68440.t1
chr1.1 exon 66135759 66135782 66135759 ID=MS.gene68440.t1.exon5;Parent=MS.gene68440.t1
chr1.1 CDS 66135759 66135782 66135759 ID=cds.MS.gene68440.t1;Parent=MS.gene68440.t1
chr1.1 exon 66135876 66135944 66135876 ID=MS.gene68440.t1.exon6;Parent=MS.gene68440.t1
chr1.1 CDS 66135876 66135944 66135876 ID=cds.MS.gene68440.t1;Parent=MS.gene68440.t1
chr1.1 exon 66136040 66136090 66136040 ID=MS.gene68440.t1.exon7;Parent=MS.gene68440.t1
chr1.1 CDS 66136040 66136090 66136040 ID=cds.MS.gene68440.t1;Parent=MS.gene68440.t1
chr1.1 exon 66136187 66136280 66136187 ID=MS.gene68440.t1.exon8;Parent=MS.gene68440.t1
chr1.1 CDS 66136187 66136280 66136187 ID=cds.MS.gene68440.t1;Parent=MS.gene68440.t1
chr1.1 exon 66136358 66136412 66136358 ID=MS.gene68440.t1.exon9;Parent=MS.gene68440.t1
chr1.1 CDS 66136358 66136412 66136358 ID=cds.MS.gene68440.t1;Parent=MS.gene68440.t1
chr1.1 exon 66136524 66136696 66136524 ID=MS.gene68440.t1.exon10;Parent=MS.gene68440.t1
chr1.1 CDS 66136524 66136696 66136524 ID=cds.MS.gene68440.t1;Parent=MS.gene68440.t1
chr1.1 exon 66136775 66136832 66136775 ID=MS.gene68440.t1.exon11;Parent=MS.gene68440.t1
chr1.1 CDS 66136775 66136832 66136775 ID=cds.MS.gene68440.t1;Parent=MS.gene68440.t1
chr1.1 exon 66136949 66137234 66136949 ID=MS.gene68440.t1.exon12;Parent=MS.gene68440.t1
chr1.1 CDS 66136949 66137234 66136949 ID=cds.MS.gene68440.t1;Parent=MS.gene68440.t1
Gene Sequence

>MS.gene68440

ATGGAAGGTGCTCCCATCTTCCTCGACTCCGATCTTCAACCTCCCGTCGCTGAAACTGGACAAGGTATCAATCTCTCTCCACTCTTCTTCTCTTTCATTTCCCAATCCTTCATTCCCATAACCCCCTAGGGTTTACTCATCCCTCTTCCCTCACTCCGAGATTAGGGTTTTCCTGTTCAATTCTGAATTTCACTTCTGTTTTGAATTTGTAATTAAGTGTTTTGTTTAACTTCAATTAAGTGATTACATAGCTATTAGATCTAACTGAGGCTCCAAAATTGAAAGGTTTATCTATATATATGCAGACATTTAGGTTTTTTAGGGTTTCGTATAGTTAATTGAAAAATGGGTTTCATTTCCTTGCAGGTCAAAAACCAAAAAGAGGTAAAATTTTGAAGGGTGATTCTGTTTCTGTTGTTGGAGCTGCTCAGAAGAAAATTCTGGCTGATATAACCAACAAGGCCCAACCAAAGCACCACCCTGCACTACCACTTCCTCCACCTTCAACTGATGTCTCCGTTGACCTCATTCTCAAGGTATATTATATATACACAATAATGTTGTTATAGTATTATGATTATTATTGATTGATTGATGATTATCACTACACTTCATGGATTTTTTGAATTTGTTTCTCAGGAAAATGAAATGATGAGGAAGCTCTTAGGTCAAAGAAAGTAATTTTTTTATCTTAATTTTGTGTCTAATTTTATGCCTTTTTATCTTCAATCAATGTAGGAATAGTTTTTGTTTACTTTGGTTTGTTCTGTTTATAGTGTGGTAATAGAATCATATAAAGCAGAGCTTCAGAAGTATCAAAGCAATTTTCAGAAATTTCGGAAGCAAAATGCAGAACTTGCACTTGCTAATACTCAAATGACGCGGGTACTACTGACTATATGTGTTTTAACTGTTGTTTATTTTAAGTTATTTGCTATTGATGATTGTTGATATTCCAATCTAAAATTGGTGATCTTTTTATCTGCAATGCAGGAGATTAGTGCAAGCAGACAAATGGTGAGTCTTGAAAACCAATGGTGCATTGACACCTGCTGCTTTCATTTCTTATTTTTTGTTATGTTTAGTGACATTCAATTTGTTGACATCCAGGTGAGGGAACTTCAGCTTGAACTAGCATGCAAAAATGGTATACTCAAAGCTATGAAATTAACATCGATGGTTAGTTTAACTCATCTCCAACCTGATGAATTTGTAAATGAGCAATTCAGAGAGCCCTGCTTTAGTTTTTGATGGTTTTGTCTTTTGATTCGTAGGAAAATGATCACAACAGAGCAAAACTGATCCATGGCATTGTTGCAGATGAGGTACTTATATATATATATTTTCCTTTATTGGCTTCTTCTGCTTGTTGGTCTTTGCTTCCAGCACACTTTCACTTAATCAATTTTTCCTTTTCTTAGAGTAAGCAGTCAGATCAAAAGTTTGAAGAAGAAAACAAGGGAGAAGAACAAAACAAAGGAGAAGCAAAAAGGAAGAGGATGTCCAAATCTCAATGTAATTAGCCTGCCGAACCCACTCTGTCTACAAAATTTAATTTATATATCTTTTAAGTAGATATTTCTCTCATGCAGCTTCTGCACCAGCTGTTGTTAAACAAGTCAAATCAGAAAAGGTTGACAGTCAAAGGTATGAGCTTGGATTTACCTTTTACACATGATTAATGTCGAATTCTAAAGTTGGACACCGGGATACTTAGGAACAAGGCAGCTTATATGTCTCCTTCAAATATGCAAACAGGTGTTCTTCGAGAAGGAAATCTGCAGCGTTGAAAGCTGGAAAATCAGGATCAACAGAAGAAGTATTTGAAATAAAATATGATGCCTCGCATCCTCTTGAAAGTTTGGCAAATGAGAAAGAATCTACATCATTAGGATCCAAAGTTCACGATGTGGCCGGACAAGATACTGAATGTAATTGCATGCCATTTATCTTTTTTTTTTTTCCTTTTTCTTTTGTTTCTCTCCTTCAACATAATTTTTTGCTTGCAGCCTCAGGACCTGCTAACACTGAACAAGTTCTTGCTGAAAGGAATGTTGAAAATAAGAGGTATACTATTATATATGAATTCCACTTTCTGAATGGATTCGAATGAATGAAATAGGAAAATGTGATTAATTTAGATAATTGAATGCCTGTTTCATGTCTTCACGAAAAAATTACAGGCATAGTTTGAGAAGGCAATCTGCCCTGTTCAGACCCGAGAAACCAGAACCAGCTGAAGACTTCTTTGATACAGAGGATCCAAAATTTGAAGTATCCAATTTATGTGATGATATGTCAGAAAGTCTTCCTACAGCATCAAGCGAAACTTCAGAAAACAATGCCTGTACACTTGATCCTCAGGTAACACGAAGATCGTCTATTGGCCGGCCTTCGCGTCGATCAGCTGTGAAGGTTGTTTCGTACAGGGAAGTTCCAATTAATCTGAAAATGCGTAGGGATAGGTGA
Protein sequence

>MS.gene68440.t1

MEGAPIFLDSDLQPPVAETGQGQKPKRGKILKGDSVSVVGAAQKKILADITNKAQPKHHPALPLPPPSTDVSVDLILKENEMMRKLLGQRNVVIESYKAELQKYQSNFQKFRKQNAELALANTQMTREISASRQMVRELQLELACKNGILKAMKLTSMENDHNRAKLIHGIVADESKQSDQKFEEENKGEEQNKGEAKRKRMSKSQSSAPAVVKQVKSEKVDSQRCSSRRKSAALKAGKSGSTEEVFEIKYDASHPLESLANEKESTSLGSKVHDVAGQDTESSGPANTEQVLAERNVENKRHSLRRQSALFRPEKPEPAEDFFDTEDPKFEVSNLCDDMSESLPTASSETSENNACTLDPQVTRRSSIGRPSRRSAVKVVSYREVPINLKMRRDR