Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69028.t1 | XP_003620655.1 | 95.2 | 62 | 3 | 0 | 1 | 62 | 12 | 73 | 2.20E-25 | 124.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69028.t1 | Q84X40 | 92.0 | 25 | 2 | 0 | 20 | 44 | 39 | 63 | 7.5e-06 | 51.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69028.t1 | G7KPU1 | 95.2 | 62 | 3 | 0 | 1 | 62 | 12 | 73 | 1.6e-25 | 124.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056354 | MS.gene69028 | 0.80923 | 2.10E-50 | -1.69E-46 |
| MS.gene056856 | MS.gene69028 | 0.819935 | 8.95E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69028.t1 | MTR_6g088280 | 93.651 | 63 | 4 | 0 | 1 | 63 | 12 | 74 | 4.44e-38 | 126 |
| MS.gene69028.t1 | MTR_8g102890 | 84.375 | 32 | 5 | 0 | 12 | 43 | 30 | 61 | 1.07e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69028.t1 | AT2G20080 | 80.000 | 35 | 7 | 0 | 10 | 44 | 29 | 63 | 6.41e-14 | 63.9 |
| MS.gene69028.t1 | AT2G20080 | 80.000 | 35 | 7 | 0 | 10 | 44 | 29 | 63 | 8.87e-14 | 63.2 |
| MS.gene69028.t1 | AT2G20080 | 80.000 | 35 | 7 | 0 | 10 | 44 | 29 | 63 | 1.27e-13 | 63.2 |
| MS.gene69028.t1 | AT4G28840 | 60.000 | 50 | 19 | 1 | 11 | 59 | 27 | 76 | 6.13e-11 | 55.5 |
Find 22 sgRNAs with CRISPR-Local
Find 88 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAACCACTTCTATGCTTCT+TGG | 0.308003 | 6.2:+86503366 | None:intergenic |
| GATAGAACAAACATTAGATT+TGG | 0.321878 | 6.2:-86504097 | MS.gene69028:CDS |
| AGGATTAGGTGTTGCTCAAT+TGG | 0.323559 | 6.2:-86505362 | MS.gene69028:CDS |
| TTACATGGTGAAATGGCTTA+TGG | 0.335235 | 6.2:-86505328 | MS.gene69028:CDS |
| AATCCTCTTTGTGGTTGCTT+TGG | 0.342523 | 6.2:+86505379 | None:intergenic |
| AATTAGATTACATGGTGAAA+TGG | 0.382742 | 6.2:-86505335 | MS.gene69028:CDS |
| CTTACATTGATAAAATTAGA+TGG | 0.387414 | 6.2:+86505277 | None:intergenic |
| TACCTTGATGAATCAAATAC+TGG | 0.388350 | 6.2:+86504064 | None:intergenic |
| AAGCAACCACAAAGAGGATT+AGG | 0.418208 | 6.2:-86505376 | MS.gene69028:CDS |
| GCAACACCTAATCCTCTTTG+TGG | 0.446963 | 6.2:+86505370 | None:intergenic |
| AGTGGATCTTCATCAAGAAA+AGG | 0.450578 | 6.2:-86505424 | MS.gene69028:CDS |
| TTGATAAAATTAGATGGATG+AGG | 0.480000 | 6.2:+86505283 | None:intergenic |
| TAATGTCACCATAGCAATCA+AGG | 0.486345 | 6.2:-86504753 | MS.gene69028:intron |
| GTGGATCTTCATCAAGAAAA+GGG | 0.515222 | 6.2:-86505423 | MS.gene69028:CDS |
| AGCCAGTATTTGATTCATCA+AGG | 0.515828 | 6.2:-86504066 | MS.gene69028:intron |
| ATGTTTGTTCTATCATATTG+AGG | 0.520659 | 6.2:+86504106 | None:intergenic |
| ATGGGGAATGAAAGAGTAAG+TGG | 0.529316 | 6.2:-86505442 | MS.gene69028:CDS |
| TTGTTCTATCATATTGAGGT+AGG | 0.542902 | 6.2:+86504110 | None:intergenic |
| GATGGATGAGGGTTGTGTAG+AGG | 0.544287 | 6.2:+86505295 | None:intergenic |
| TGATAAAATTAGATGGATGA+GGG | 0.547151 | 6.2:+86505284 | None:intergenic |
| GGATGAGGGTTGTGTAGAGG+AGG | 0.604170 | 6.2:+86505298 | None:intergenic |
| AAACCAAAGCAACCACAAAG+AGG | 0.610893 | 6.2:-86505382 | MS.gene69028:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTATTTATTTTTTTATAAAT+AGG | - | chr6.2:86505411-86505430 | MS.gene69028:CDS | 0.0% |
| !!! | AATTTTATTTATGCTTTTAA+TGG | - | chr6.2:86503912-86503931 | MS.gene69028:intron | 10.0% |
| !!! | GAAAAAAATATATTTTTACT+TGG | - | chr6.2:86503654-86503673 | MS.gene69028:intron | 10.0% |
| !!! | TAAAAGCATAAATAAAATTT+GGG | + | chr6.2:86503911-86503930 | None:intergenic | 10.0% |
| !!! | TTAAAAGCATAAATAAAATT+TGG | + | chr6.2:86503912-86503931 | None:intergenic | 10.0% |
| !!! | TTCATTTTTTTGTAATTTAT+TGG | + | chr6.2:86505375-86505394 | None:intergenic | 10.0% |
| !!! | ATTTTTCAATTTTATCTGAT+TGG | + | chr6.2:86504506-86504525 | None:intergenic | 15.0% |
| !!! | TTTACATTTTGATACATTAT+CGG | + | chr6.2:86505319-86505338 | None:intergenic | 15.0% |
| !! | AAATCATAGAAAATATACCT+AGG | - | chr6.2:86505336-86505355 | MS.gene69028:CDS | 20.0% |
| !! | ACAAAAACAATATATTCAAG+TGG | - | chr6.2:86504038-86504057 | MS.gene69028:intron | 20.0% |
| !! | ATCCAAAAATAACATTTCTA+GGG | + | chr6.2:86504199-86504218 | None:intergenic | 20.0% |
| !! | ATGTAAGAATTAATTCTCTA+AGG | + | chr6.2:86504530-86504549 | None:intergenic | 20.0% |
| !! | CAAAAACAATATATTCAAGT+GGG | - | chr6.2:86504039-86504058 | MS.gene69028:intron | 20.0% |
| !! | CTTACATTGATAAAATTAGA+TGG | + | chr6.2:86503544-86503563 | None:intergenic | 20.0% |
| !! | GAAAATGAATTCTTACATAT+AGG | + | chr6.2:86505011-86505030 | None:intergenic | 20.0% |
| !! | TGATGATTACAATTAATTGA+CGG | + | chr6.2:86505205-86505224 | None:intergenic | 20.0% |
| !! | TTGGAAAAAATTAGATTACA+TGG | - | chr6.2:86503475-86503494 | MS.gene69028:intron | 20.0% |
| !! | TTTATCTCAAATCAGATTAT+GGG | - | chr6.2:86504936-86504955 | MS.gene69028:intron | 20.0% |
| !!! | AATTAATTGTGATTTGTTGA+CGG | - | chr6.2:86505277-86505296 | MS.gene69028:intron | 20.0% |
| !!! | TAATAATAATGGAACTAATG+TGG | + | chr6.2:86504794-86504813 | None:intergenic | 20.0% |
| !!! | TATATTTTTACTTGGATTTG+AGG | - | chr6.2:86503662-86503681 | MS.gene69028:intron | 20.0% |
| !!! | TGCTTTAATCTTTTTTTTGA+AGG | - | chr6.2:86504339-86504358 | MS.gene69028:intron | 20.0% |
| !!! | TGTAATTTTTTTGCTTTTCT+TGG | - | chr6.2:86503883-86503902 | MS.gene69028:intron | 20.0% |
| ! | AACAAAGGAAAGAAAAATCA+AGG | + | chr6.2:86504107-86504126 | None:intergenic | 25.0% |
| ! | AATTAGATTACATGGTGAAA+TGG | - | chr6.2:86503483-86503502 | MS.gene69028:intron | 25.0% |
| ! | ACAAAGGAAAGAAAAATCAA+GGG | + | chr6.2:86504106-86504125 | None:intergenic | 25.0% |
| ! | ATGTTTGTTCTATCATATTG+AGG | + | chr6.2:86504715-86504734 | None:intergenic | 25.0% |
| ! | ATTACACTTACCATAATGTT+TGG | + | chr6.2:86503869-86503888 | None:intergenic | 25.0% |
| ! | CTTACATATAGGTTAAGTAA+TGG | + | chr6.2:86505000-86505019 | None:intergenic | 25.0% |
| ! | CTTTAATCATAATCAGCTTT+AGG | - | chr6.2:86505244-86505263 | MS.gene69028:intron | 25.0% |
| ! | CTTTATCTCAAATCAGATTA+TGG | - | chr6.2:86504935-86504954 | MS.gene69028:intron | 25.0% |
| ! | GATAGAACAAACATTAGATT+TGG | - | chr6.2:86504721-86504740 | MS.gene69028:intron | 25.0% |
| ! | GATATTAAGAAGAGATTACA+AGG | + | chr6.2:86504644-86504663 | None:intergenic | 25.0% |
| ! | GATCCAAAAATAACATTTCT+AGG | + | chr6.2:86504200-86504219 | None:intergenic | 25.0% |
| ! | TGATAAAATTAGATGGATGA+GGG | + | chr6.2:86503537-86503556 | None:intergenic | 25.0% |
| ! | TGATGAATTTCTTGCTTATA+TGG | - | chr6.2:86504906-86504925 | MS.gene69028:intron | 25.0% |
| ! | TTGATAAAATTAGATGGATG+AGG | + | chr6.2:86503538-86503557 | None:intergenic | 25.0% |
| ! | TTGGTATATTATTAGAACCT+AGG | + | chr6.2:86505356-86505375 | None:intergenic | 25.0% |
| !! | ATTTTCTTTTTCTATGCAGA+TGG | - | chr6.2:86504684-86504703 | MS.gene69028:intron | 25.0% |
| !! | TTTTCTTTTTCTATGCAGAT+GGG | - | chr6.2:86504685-86504704 | MS.gene69028:intron | 25.0% |
| !!! | AACCCTAGAAATGTTATTTT+TGG | - | chr6.2:86504194-86504213 | MS.gene69028:intron | 25.0% |
| !!! | AATAACATTTCTAGGGTTAT+TGG | + | chr6.2:86504192-86504211 | None:intergenic | 25.0% |
| !!! | ACTAGTTTGATTTGAGAAAA+GGG | + | chr6.2:86503584-86503603 | None:intergenic | 25.0% |
| !!! | AGAAATGTTATTTTTGGATC+TGG | - | chr6.2:86504200-86504219 | MS.gene69028:intron | 25.0% |
| !!! | GAAATGTTATTTTTGGATCT+GGG | - | chr6.2:86504201-86504220 | MS.gene69028:intron | 25.0% |
| AATAGTTACCTTGATTGCTA+TGG | + | chr6.2:86504076-86504095 | None:intergenic | 30.0% | |
| AATTGTGTATTACTAACCCT+AGG | - | chr6.2:86504264-86504283 | MS.gene69028:intron | 30.0% | |
| ATGTCATGTCATTACAACAA+AGG | + | chr6.2:86504122-86504141 | None:intergenic | 30.0% | |
| CTCATCACAAAAGAATGAAA+AGG | + | chr6.2:86503950-86503969 | None:intergenic | 30.0% | |
| GAGTAAGAAAAAGATGATGA+TGG | + | chr6.2:86503806-86503825 | None:intergenic | 30.0% | |
| GTTAAGTAATGGTTTAGTTG+TGG | + | chr6.2:86504989-86505008 | None:intergenic | 30.0% | |
| TACCTTGATGAATCAAATAC+TGG | + | chr6.2:86504757-86504776 | None:intergenic | 30.0% | |
| TTGTTCTATCATATTGAGGT+AGG | + | chr6.2:86504711-86504730 | None:intergenic | 30.0% | |
| ! | AATAATGGAACTAATGTGGA+TGG | + | chr6.2:86504790-86504809 | None:intergenic | 30.0% |
| ! | TTTGTTTCTCATGATCATGT+AGG | - | chr6.2:86503745-86503764 | MS.gene69028:intron | 30.0% |
| !! | GACTAGTTTGATTTGAGAAA+AGG | + | chr6.2:86503585-86503604 | None:intergenic | 30.0% |
| !! | GATGATGGTATTGATGAATA+TGG | + | chr6.2:86503791-86503810 | None:intergenic | 30.0% |
| AAACCAATTTCTTCTCACCT+AGG | + | chr6.2:86504284-86504303 | None:intergenic | 35.0% | |
| AAAGGGAGAGAGAAATTAAG+AGG | + | chr6.2:86503567-86503586 | None:intergenic | 35.0% | |
| AACCAATTTCTTCTCACCTA+GGG | + | chr6.2:86504283-86504302 | None:intergenic | 35.0% | |
| AAGTAATGGTTTAGTTGTGG+TGG | + | chr6.2:86504986-86505005 | None:intergenic | 35.0% | |
| AGCCAGTATTTGATTCATCA+AGG | - | chr6.2:86504752-86504771 | MS.gene69028:intron | 35.0% | |
| AGTGGATCTTCATCAAGAAA+AGG | - | chr6.2:86503394-86503413 | MS.gene69028:intron | 35.0% | |
| GTGGATCTTCATCAAGAAAA+GGG | - | chr6.2:86503395-86503414 | MS.gene69028:intron | 35.0% | |
| TAATGTCACCATAGCAATCA+AGG | - | chr6.2:86504065-86504084 | MS.gene69028:intron | 35.0% | |
| TGGCTTTCAACCAAACATTA+TGG | - | chr6.2:86503856-86503875 | MS.gene69028:intron | 35.0% | |
| TTACATGGTGAAATGGCTTA+TGG | - | chr6.2:86503490-86503509 | MS.gene69028:intron | 35.0% | |
| AAACCAAAGCAACCACAAAG+AGG | - | chr6.2:86503436-86503455 | MS.gene69028:intron | 40.0% | |
| AACCCTAGGTGAGAAGAAAT+TGG | - | chr6.2:86504278-86504297 | MS.gene69028:intron | 40.0% | |
| AAGCAACCACAAAGAGGATT+AGG | - | chr6.2:86503442-86503461 | MS.gene69028:intron | 40.0% | |
| AATCCTCTTTGTGGTTGCTT+TGG | + | chr6.2:86503442-86503461 | None:intergenic | 40.0% | |
| ACATCATACTCAGCTTCACA+TGG | - | chr6.2:86503836-86503855 | MS.gene69028:intron | 40.0% | |
| ATGGGGAATGAAAGAGTAAG+TGG | - | chr6.2:86503376-86503395 | MS.gene69028:CDS | 40.0% | |
| ! | ATTTTCCACAGTAGCACACT+AGG | + | chr6.2:86504469-86504488 | None:intergenic | 40.0% |
| !! | AGGATTAGGTGTTGCTCAAT+TGG | - | chr6.2:86503456-86503475 | MS.gene69028:intron | 40.0% |
| GCAACACCTAATCCTCTTTG+TGG | + | chr6.2:86503451-86503470 | None:intergenic | 45.0% | |
| ! | TGACTCTCTTTTGGTCTGGT+TGG | - | chr6.2:86504225-86504244 | MS.gene69028:intron | 45.0% |
| ! | TTATGCCTAGTGTGCTACTG+TGG | - | chr6.2:86504461-86504480 | MS.gene69028:intron | 45.0% |
| !! | AGTTGTGGTGGATTGAGAAG+TGG | + | chr6.2:86504974-86504993 | None:intergenic | 45.0% |
| !! | AATAAATAAAAAATTATTGA+AGG | + | chr6.2:86505400-86505419 | None:intergenic | 5.0% |
| !! | CATTTAAAAAAAAATAAAAT+TGG | + | chr6.2:86503610-86503629 | None:intergenic | 5.0% |
| !! | TTGATTATTATTAATAATAA+TGG | + | chr6.2:86504805-86504824 | None:intergenic | 5.0% |
| !!! | AAACAAATTAATTTTTTTTT+TGG | + | chr6.2:86503732-86503751 | None:intergenic | 5.0% |
| GATGGATGAGGGTTGTGTAG+AGG | + | chr6.2:86503526-86503545 | None:intergenic | 50.0% | |
| !! | GATCTGGGCTGACTCTCTTT+TGG | - | chr6.2:86504216-86504235 | MS.gene69028:intron | 50.0% |
| !! | GGTGGATTGAGAAGTGGCAT+TGG | + | chr6.2:86504968-86504987 | None:intergenic | 50.0% |
| GGATGAGGGTTGTGTAGAGG+AGG | + | chr6.2:86503523-86503542 | None:intergenic | 55.0% | |
| !! | GGGCTGACTCTCTTTTGGTC+TGG | - | chr6.2:86504221-86504240 | MS.gene69028:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.2 | gene | 86503376 | 86505464 | 86503376 | ID=MS.gene69028 |
| chr6.2 | mRNA | 86503376 | 86505464 | 86503376 | ID=MS.gene69028.t1;Parent=MS.gene69028 |
| chr6.2 | exon | 86505282 | 86505464 | 86505282 | ID=MS.gene69028.t1.exon1;Parent=MS.gene69028.t1 |
| chr6.2 | CDS | 86505282 | 86505464 | 86505282 | ID=cds.MS.gene69028.t1;Parent=MS.gene69028.t1 |
| chr6.2 | exon | 86504754 | 86504761 | 86504754 | ID=MS.gene69028.t1.exon2;Parent=MS.gene69028.t1 |
| chr6.2 | CDS | 86504754 | 86504761 | 86504754 | ID=cds.MS.gene69028.t1;Parent=MS.gene69028.t1 |
| chr6.2 | exon | 86504067 | 86504137 | 86504067 | ID=MS.gene69028.t1.exon3;Parent=MS.gene69028.t1 |
| chr6.2 | CDS | 86504067 | 86504137 | 86504067 | ID=cds.MS.gene69028.t1;Parent=MS.gene69028.t1 |
| chr6.2 | exon | 86503376 | 86503407 | 86503376 | ID=MS.gene69028.t1.exon4;Parent=MS.gene69028.t1 |
| chr6.2 | CDS | 86503376 | 86503407 | 86503376 | ID=cds.MS.gene69028.t1;Parent=MS.gene69028.t1 |
| Gene Sequence |
| Protein sequence |
