AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene69188


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene69188.t1 MTR_3g111780 94.718 284 14 1 1 284 1 283 0.0 501
MS.gene69188.t1 MTR_8g103520 50.000 72 36 0 193 264 218 289 5.16e-20 87.8
MS.gene69188.t1 MTR_0907s0020 52.941 68 32 0 197 264 100 167 8.28e-20 85.1
MS.gene69188.t1 MTR_5g075210 58.462 65 27 0 199 263 280 344 6.43e-19 85.9
MS.gene69188.t1 MTR_7g105790 53.623 69 32 0 202 270 113 181 5.47e-18 80.5
MS.gene69188.t1 MTR_7g072875 32.468 154 80 2 129 260 129 280 5.60e-18 82.4
MS.gene69188.t1 MTR_4g052040 53.846 65 30 0 200 264 100 164 1.82e-17 78.6
MS.gene69188.t1 MTR_5g079710 50.725 69 34 0 196 264 88 156 9.95e-17 78.2
MS.gene69188.t1 MTR_7g072630 26.829 287 155 8 27 260 58 342 1.09e-16 79.3
MS.gene69188.t1 MTR_8g087530 47.692 65 34 0 199 263 258 322 6.48e-16 77.0
MS.gene69188.t1 MTR_2g018030 46.774 62 33 0 199 260 277 338 9.80e-16 76.6
MS.gene69188.t1 MTR_4g028930 50.820 61 30 0 200 260 315 375 6.36e-15 74.3
MS.gene69188.t1 MTR_4g052060 51.667 60 29 0 205 264 99 158 1.91e-13 67.8
MS.gene69188.t1 MTR_1g111640 45.161 62 34 0 198 259 136 197 3.67e-11 62.4
MS.gene69188.t1 MTR_4g132740 37.500 88 44 1 178 265 71 147 5.82e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene69188.t1 AT5G19650 62.121 66 25 0 199 264 154 219 7.03e-23 94.4
MS.gene69188.t1 AT2G18500 61.538 65 25 0 199 263 226 290 2.81e-20 89.0
MS.gene69188.t1 AT3G52525 55.385 65 29 0 197 261 64 128 2.10e-18 80.5
MS.gene69188.t1 AT2G30400 52.308 65 31 0 199 263 252 316 3.14e-18 83.6
MS.gene69188.t1 AT5G58360 53.846 65 30 0 199 263 224 288 3.68e-17 80.1
MS.gene69188.t1 AT2G36026 55.000 60 27 0 205 264 92 151 4.75e-17 77.8
MS.gene69188.t1 AT1G06920 33.010 103 64 1 167 264 206 308 3.53e-16 77.8
MS.gene69188.t1 AT5G01840 48.529 68 35 0 203 270 202 269 3.71e-15 73.9
MS.gene69188.t1 AT5G22240 44.118 68 38 0 196 263 93 160 4.25e-13 67.0
MS.gene69188.t1 AT4G18830 45.902 61 33 0 199 259 282 342 7.42e-12 65.5

Find 67 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGGCAGGGCCTGGATTCC+CGG 0.272464 3.4:+96907434 None:intergenic
CTCAAATTATAGTGATAAAA+AGG 0.274257 3.4:-96907130 MS.gene69188:CDS
GGTCCATTCTTCTAGTACTA+TGG 0.311039 3.4:-96907175 MS.gene69188:CDS
TGCGAGCTTTCATGGAGATT+TGG 0.332540 3.4:-96906753 MS.gene69188:CDS
AGTAATTGTGCGAGCTTTCA+TGG 0.344864 3.4:-96906761 MS.gene69188:CDS
GACACGTTGCATTGGTGTTC+CGG 0.349518 3.4:+96907345 None:intergenic
CACCGGAGAAAGACGGTATA+TGG 0.359937 3.4:+96907416 None:intergenic
TCCTCTTTCCCCGGGAATCC+AGG 0.367021 3.4:-96907444 MS.gene69188:CDS
TTTGGCAACAAATGTTCGTT+TGG 0.381425 3.4:-96906735 MS.gene69188:CDS
GGCGACGAAGCAGGAAGGTT+TGG 0.382600 3.4:+96907366 None:intergenic
TTATCACTATAATTTGAGAT+AGG 0.385663 3.4:+96907135 None:intergenic
ATACCCTATGTCATATGCTT+TGG 0.387292 3.4:+96907389 None:intergenic
GGCAAAAGAAAGCTTTGCTT+TGG 0.397965 3.4:-96906923 MS.gene69188:CDS
AGTGAGGTTTCAAAGTATTA+CGG 0.405418 3.4:-96907046 MS.gene69188:CDS
ATTTAACTAGCTCGAATAGA+AGG 0.424510 3.4:-96907098 MS.gene69188:CDS
AATGACCTTTCCTCTTTCCC+CGG 0.427656 3.4:-96907453 MS.gene69188:CDS
ACTTGACATAACCTTCGTTT+CGG 0.428668 3.4:+96906960 None:intergenic
GATGTCCGAGGCCAAGGACT+TGG 0.437700 3.4:-96906833 MS.gene69188:CDS
ATGGCAGGGCCTGGATTCCC+GGG 0.439698 3.4:+96907435 None:intergenic
GTTAGAGATGATAGAAGAGA+TGG 0.441612 3.4:-96906857 MS.gene69188:CDS
TTCTTGCTTAGCAAGATTCT+CGG 0.449560 3.4:-96907208 MS.gene69188:CDS
GAAGGTTATGTCAAGTAGAA+TGG 0.463702 3.4:-96906953 MS.gene69188:CDS
GAAGGTTATGTCAAGTAGAA+TGG 0.463702 3.4:-96907001 MS.gene69188:CDS
CTATGGATAGTACTAAAGTT+AGG 0.470027 3.4:-96907158 MS.gene69188:CDS
TGAAGAAGGTTGAGAGAGTG+AGG 0.472397 3.4:-96907062 MS.gene69188:CDS
CAATAGTTGCTCCAAGTCCT+TGG 0.478606 3.4:+96906822 None:intergenic
TCAAACAAATGTTCAAACTA+AGG 0.481359 3.4:-96906689 MS.gene69188:CDS
GGGGAAAGAGGAAAGGTCAT+TGG 0.484366 3.4:+96907455 None:intergenic
AGACGGTATATGGCAGGGCC+TGG 0.484707 3.4:+96907426 None:intergenic
ACTAGAAGAATGGACCTTCT+CGG 0.488884 3.4:+96907182 None:intergenic
GCCTGGATTCCCGGGGAAAG+AGG 0.494617 3.4:+96907443 None:intergenic
TATCCATAGTACTAGAAGAA+TGG 0.495158 3.4:+96907172 None:intergenic
AGAAACTCACTTCTTCTTGA+GGG 0.501729 3.4:+96907270 None:intergenic
TGGTGTTCCGGCGACGAAGC+AGG 0.502249 3.4:+96907357 None:intergenic
GACGATAAGTCGTGTGAAGA+AGG 0.503045 3.4:-96907076 MS.gene69188:CDS
GATGGAGATGTCCGAGGCCA+AGG 0.503048 3.4:-96906839 MS.gene69188:CDS
TGCCATATACCGTCTTTCTC+CGG 0.503136 3.4:-96907418 MS.gene69188:CDS
GAGCGACACATTTGAAAAGA+AGG 0.503682 3.4:+96907477 None:intergenic
AAACCTTCCTGCTTCGTCGC+CGG 0.514759 3.4:-96907364 MS.gene69188:CDS
TTGCTCCAAGTCCTTGGCCT+CGG 0.515968 3.4:+96906828 None:intergenic
AGCGACACATTTGAAAAGAA+GGG 0.522344 3.4:+96907478 None:intergenic
ATGACCTTTCCTCTTTCCCC+GGG 0.522963 3.4:-96907452 MS.gene69188:CDS
GGAGAAAGACGGTATATGGC+AGG 0.531009 3.4:+96907420 None:intergenic
TAGAAACTCACTTCTTCTTG+AGG 0.531127 3.4:+96907269 None:intergenic
TCATATGCTTTGGAGTTCAC+CGG 0.541099 3.4:+96907399 None:intergenic
CTAAATTCTAGAGATTATCA+TGG 0.544872 3.4:-96906784 MS.gene69188:CDS
GTCAAGTAGAATGGAGGAGA+AGG 0.546067 3.4:-96906944 MS.gene69188:CDS
GATTTCTTGAAGTCTTCGTA+AGG 0.562850 3.4:+96906883 None:intergenic
AATTGATAAAATCAATGAAG+AGG 0.573253 3.4:-96907247 MS.gene69188:CDS
AAGATTCTCGGAAGCCGAGA+AGG 0.578226 3.4:-96907196 MS.gene69188:CDS
AACTCCAAAGCATATGACAT+AGG 0.582440 3.4:-96907393 MS.gene69188:CDS
TCTTTGATGACACGTTGCAT+TGG 0.597146 3.4:+96907337 None:intergenic
TCTTCTTGAGGGAACGTCGG+AGG 0.601171 3.4:+96907281 None:intergenic
ACTCCAAAGCATATGACATA+GGG 0.601832 3.4:-96907392 MS.gene69188:CDS
TCGAAGAGGAACCGAAACGA+AGG 0.602284 3.4:-96906971 MS.gene69188:CDS
ATTAAGAAAGTGAAATCCAA+AGG 0.608408 3.4:-96907315 MS.gene69188:CDS
TGGAGTTCACCGGAGAAAGA+CGG 0.608559 3.4:+96907409 None:intergenic
GTTCCGGCGACGAAGCAGGA+AGG 0.630275 3.4:+96907361 None:intergenic
GATTCCCGGGGAAAGAGGAA+AGG 0.637825 3.4:+96907448 None:intergenic
GAGAAAGACGGTATATGGCA+GGG 0.639308 3.4:+96907421 None:intergenic
ACTTCTTCTTGAGGGAACGT+CGG 0.649804 3.4:+96907278 None:intergenic
AGTATTACGGAGAATCAAAG+AGG 0.650942 3.4:-96907033 MS.gene69188:CDS
TGGCAGGGCCTGGATTCCCG+GGG 0.653477 3.4:+96907436 None:intergenic
GGTTATGTCAAGTAGAATGG+AGG 0.656874 3.4:-96906950 MS.gene69188:CDS
AGAATGGAAGAGAATCGAAG+AGG 0.657497 3.4:-96906985 MS.gene69188:CDS
TCAAAGAGGAAAAGAAACGA+AGG 0.672215 3.4:-96907019 MS.gene69188:CDS
AGAAGAGATGGAGATGTCCG+AGG 0.675634 3.4:-96906845 MS.gene69188:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTGATAAAATCAATGAAG+AGG - chr3.4:96906938-96906957 MS.gene69188:CDS 20.0%
!! CTCAAATTATAGTGATAAAA+AGG - chr3.4:96907055-96907074 MS.gene69188:CDS 20.0%
!!! TTATCACTATAATTTGAGAT+AGG + chr3.4:96907053-96907072 None:intergenic 20.0%
! ATTAAGAAAGTGAAATCCAA+AGG - chr3.4:96906870-96906889 MS.gene69188:CDS 25.0%
! TCAAACAAATGTTCAAACTA+AGG - chr3.4:96907496-96907515 MS.gene69188:CDS 25.0%
!! CTAAATTCTAGAGATTATCA+TGG - chr3.4:96907401-96907420 MS.gene69188:CDS 25.0%
!!! TGAAGATTTTTTACTGACTT+AGG + chr3.4:96907479-96907498 None:intergenic 25.0%
ATTTAACTAGCTCGAATAGA+AGG - chr3.4:96907087-96907106 MS.gene69188:CDS 30.0%
CTATGGATAGTACTAAAGTT+AGG - chr3.4:96907027-96907046 MS.gene69188:CDS 30.0%
TATCCATAGTACTAGAAGAA+TGG + chr3.4:96907016-96907035 None:intergenic 30.0%
! AGTGAGGTTTCAAAGTATTA+CGG - chr3.4:96907139-96907158 MS.gene69188:CDS 30.0%
AACTCCAAAGCATATGACAT+AGG - chr3.4:96906792-96906811 MS.gene69188:CDS 35.0%
ACTCCAAAGCATATGACATA+GGG - chr3.4:96906793-96906812 MS.gene69188:CDS 35.0%
ACTTGACATAACCTTCGTTT+CGG + chr3.4:96907180-96907199 None:intergenic 35.0%
AGAAACTCACTTCTTCTTGA+GGG + chr3.4:96906918-96906937 None:intergenic 35.0%
AGCGACACATTTGAAAAGAA+GGG + chr3.4:96906710-96906729 None:intergenic 35.0%
AGTATTACGGAGAATCAAAG+AGG - chr3.4:96907152-96907171 MS.gene69188:CDS 35.0%
ATACCCTATGTCATATGCTT+TGG + chr3.4:96906799-96906818 None:intergenic 35.0%
GAAGGTTATGTCAAGTAGAA+TGG - chr3.4:96907184-96907203 MS.gene69188:CDS 35.0%
GATTTCTTGAAGTCTTCGTA+AGG + chr3.4:96907305-96907324 None:intergenic 35.0%
GTTAGAGATGATAGAAGAGA+TGG - chr3.4:96907328-96907347 MS.gene69188:CDS 35.0%
TAGAAACTCACTTCTTCTTG+AGG + chr3.4:96906919-96906938 None:intergenic 35.0%
TCAAAGAGGAAAAGAAACGA+AGG - chr3.4:96907166-96907185 MS.gene69188:CDS 35.0%
TGCTTTGGTGAAAAAGTCAA+AGG - chr3.4:96907277-96907296 MS.gene69188:CDS 35.0%
TTCTTGCTTAGCAAGATTCT+CGG - chr3.4:96906977-96906996 MS.gene69188:CDS 35.0%
TTTGGCAACAAATGTTCGTT+TGG - chr3.4:96907450-96907469 MS.gene69188:CDS 35.0%
!!! GCAACTATTGCAGTGTTTTT+TGG - chr3.4:96907376-96907395 MS.gene69188:CDS 35.0%
ACTAGAAGAATGGACCTTCT+CGG + chr3.4:96907006-96907025 None:intergenic 40.0%
AGAATGGAAGAGAATCGAAG+AGG - chr3.4:96907200-96907219 MS.gene69188:CDS 40.0%
AGTAATTGTGCGAGCTTTCA+TGG - chr3.4:96907424-96907443 MS.gene69188:CDS 40.0%
GAGCGACACATTTGAAAAGA+AGG + chr3.4:96906711-96906730 None:intergenic 40.0%
GGCAAAAGAAAGCTTTGCTT+TGG - chr3.4:96907262-96907281 MS.gene69188:CDS 40.0%
GGTCCATTCTTCTAGTACTA+TGG - chr3.4:96907010-96907029 MS.gene69188:CDS 40.0%
GGTTATGTCAAGTAGAATGG+AGG - chr3.4:96907187-96907206 MS.gene69188:CDS 40.0%
! TCTTTGATGACACGTTGCAT+TGG + chr3.4:96906851-96906870 None:intergenic 40.0%
!! TCATATGCTTTGGAGTTCAC+CGG + chr3.4:96906789-96906808 None:intergenic 40.0%
AATGACCTTTCCTCTTTCCC+CGG - chr3.4:96906732-96906751 MS.gene69188:CDS 45.0%
ACTTCTTCTTGAGGGAACGT+CGG + chr3.4:96906910-96906929 None:intergenic 45.0%
CAATAGTTGCTCCAAGTCCT+TGG + chr3.4:96907366-96907385 None:intergenic 45.0%
GACGATAAGTCGTGTGAAGA+AGG - chr3.4:96907109-96907128 MS.gene69188:CDS 45.0%
GAGAAAGACGGTATATGGCA+GGG + chr3.4:96906767-96906786 None:intergenic 45.0%
GTCAAGTAGAATGGAGGAGA+AGG - chr3.4:96907241-96907260 MS.gene69188:CDS 45.0%
TGAAGAAGGTTGAGAGAGTG+AGG - chr3.4:96907123-96907142 MS.gene69188:CDS 45.0%
TGCCATATACCGTCTTTCTC+CGG - chr3.4:96906767-96906786 MS.gene69188:CDS 45.0%
TGCGAGCTTTCATGGAGATT+TGG - chr3.4:96907432-96907451 MS.gene69188:CDS 45.0%
AAGATTCTCGGAAGCCGAGA+AGG - chr3.4:96906989-96907008 MS.gene69188:CDS 50.0%
AGAAGAGATGGAGATGTCCG+AGG - chr3.4:96907340-96907359 MS.gene69188:CDS 50.0%
ATGACCTTTCCTCTTTCCCC+GGG - chr3.4:96906733-96906752 MS.gene69188:CDS 50.0%
CACCGGAGAAAGACGGTATA+TGG + chr3.4:96906772-96906791 None:intergenic 50.0%
GGAGAAAGACGGTATATGGC+AGG + chr3.4:96906768-96906787 None:intergenic 50.0%
GGGGAAAGAGGAAAGGTCAT+TGG + chr3.4:96906733-96906752 None:intergenic 50.0%
TCGAAGAGGAACCGAAACGA+AGG - chr3.4:96907214-96907233 MS.gene69188:CDS 50.0%
TGGAGTTCACCGGAGAAAGA+CGG + chr3.4:96906779-96906798 None:intergenic 50.0%
! GACACGTTGCATTGGTGTTC+CGG + chr3.4:96906843-96906862 None:intergenic 50.0%
AAACCTTCCTGCTTCGTCGC+CGG - chr3.4:96906821-96906840 MS.gene69188:CDS 55.0%
GATTCCCGGGGAAAGAGGAA+AGG + chr3.4:96906740-96906759 None:intergenic 55.0%
TCTTCTTGAGGGAACGTCGG+AGG + chr3.4:96906907-96906926 None:intergenic 55.0%
TTGCTCCAAGTCCTTGGCCT+CGG + chr3.4:96907360-96907379 None:intergenic 55.0%
!!! GAACGTCGGAGGTGCTTTTG+CGG + chr3.4:96906896-96906915 None:intergenic 55.0%
AGACGGTATATGGCAGGGCC+TGG + chr3.4:96906762-96906781 None:intergenic 60.0%
GATGGAGATGTCCGAGGCCA+AGG - chr3.4:96907346-96907365 MS.gene69188:CDS 60.0%
GATGTCCGAGGCCAAGGACT+TGG - chr3.4:96907352-96907371 MS.gene69188:CDS 60.0%
GGCGACGAAGCAGGAAGGTT+TGG + chr3.4:96906822-96906841 None:intergenic 60.0%
TATGGCAGGGCCTGGATTCC+CGG + chr3.4:96906754-96906773 None:intergenic 60.0%
TCCTCTTTCCCCGGGAATCC+AGG - chr3.4:96906741-96906760 MS.gene69188:CDS 60.0%
!!! GGAGGTGCTTTTGCGGCCTT+TGG + chr3.4:96906889-96906908 None:intergenic 60.0%
ATGGCAGGGCCTGGATTCCC+GGG + chr3.4:96906753-96906772 None:intergenic 65.0%
GCCTGGATTCCCGGGGAAAG+AGG + chr3.4:96906745-96906764 None:intergenic 65.0%
GTTCCGGCGACGAAGCAGGA+AGG + chr3.4:96906827-96906846 None:intergenic 65.0%
TGGTGTTCCGGCGACGAAGC+AGG + chr3.4:96906831-96906850 None:intergenic 65.0%
TGGCAGGGCCTGGATTCCCG+GGG + chr3.4:96906752-96906771 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 96906675 96907532 96906675 ID=MS.gene69188
chr3.4 mRNA 96906675 96907532 96906675 ID=MS.gene69188.t1;Parent=MS.gene69188
chr3.4 exon 96906675 96907532 96906675 ID=MS.gene69188.t1.exon1;Parent=MS.gene69188.t1
chr3.4 CDS 96906675 96907532 96906675 ID=cds.MS.gene69188.t1;Parent=MS.gene69188.t1
Gene Sequence

>MS.gene69188

ATGTCTAAACGCTTTAAGATGAAATTTACAATCCCTTCTTTTCAAATGTGTCGCTCCAATGACCTTTCCTCTTTCCCCGGGAATCCAGGCCCTGCCATATACCGTCTTTCTCCGGTGAACTCCAAAGCATATGACATAGGGTATCCAAACCTTCCTGCTTCGTCGCCGGAACACCAATGCAACGTGTCATCAAAGATTAAGAAAGTGAAATCCAAAGGCCGCAAAAGCACCTCCGACGTTCCCTCAAGAAGAAGTGAGTTTCTAATTGATAAAATCAATGAAGAGGAAAGTGAATCATTGATTTCTTGCTTAGCAAGATTCTCGGAAGCCGAGAAGGTCCATTCTTCTAGTACTATGGATAGTACTAAAGTTAGGCCTATCTCAAATTATAGTGATAAAAAGGAGAATAAAGATTTAACTAGCTCGAATAGAAGGACGATAAGTCGTGTGAAGAAGGTTGAGAGAGTGAGGTTTCAAAGTATTACGGAGAATCAAAGAGGAAAAGAAACGAAGGTTATGTCAAGTAGAATGGAAGAGAATCGAAGAGGAACCGAAACGAAGGTTATGTCAAGTAGAATGGAGGAGAAGGCAAAAGAAAGCTTTGCTTTGGTGAAAAAGTCAAAGGATCCTTACGAAGACTTCAAGAAATCAATGTTAGAGATGATAGAAGAGATGGAGATGTCCGAGGCCAAGGACTTGGAGCAACTATTGCAGTGTTTTTTGGCTCTAAATTCTAGAGATTATCATGGAGTAATTGTGCGAGCTTTCATGGAGATTTGGCAACAAATGTTCGTTTGGAATCCTAAGTCAGTAAAAAATCTTCAAACAAATGTTCAAACTAAGGATGTAGAAAATTAG

Protein sequence

>MS.gene69188.t1

MSKRFKMKFTIPSFQMCRSNDLSSFPGNPGPAIYRLSPVNSKAYDIGYPNLPASSPEHQCNVSSKIKKVKSKGRKSTSDVPSRRSEFLIDKINEEESESLISCLARFSEAEKVHSSSTMDSTKVRPISNYSDKKENKDLTSSNRRTISRVKKVERVRFQSITENQRGKETKVMSSRMEENRRGTETKVMSSRMEEKAKESFALVKKSKDPYEDFKKSMLEMIEEMEMSEAKDLEQLLQCFLALNSRDYHGVIVRAFMEIWQQMFVWNPKSVKNLQTNVQTKDVEN