Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69361.t1 | XP_003603835.1 | 96.4 | 247 | 7 | 2 | 1 | 247 | 1 | 245 | 2.50E-118 | 434.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69361.t1 | Q9SGS5 | 37.9 | 277 | 119 | 9 | 6 | 246 | 13 | 272 | 1.9e-21 | 104.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69361.t1 | G7J5W8 | 96.4 | 247 | 7 | 2 | 1 | 247 | 1 | 245 | 1.8e-118 | 434.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050363 | MS.gene69361 | 0.823296 | 1.50E-53 | -1.69E-46 |
| MS.gene052091 | MS.gene69361 | 0.809277 | 2.05E-50 | -1.69E-46 |
| MS.gene055549 | MS.gene69361 | 0.807813 | 4.21E-50 | -1.69E-46 |
| MS.gene055550 | MS.gene69361 | 0.816612 | 5.05E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69361.t1 | MTR_3g114750 | 95.951 | 247 | 8 | 2 | 1 | 247 | 1 | 245 | 4.57e-176 | 484 |
| MS.gene69361.t1 | MTR_1g026110 | 50.388 | 258 | 97 | 10 | 2 | 246 | 4 | 243 | 5.65e-66 | 205 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69361.t1 | AT1G76070 | 30.627 | 271 | 131 | 9 | 14 | 246 | 21 | 272 | 2.28e-28 | 108 |
| MS.gene69361.t1 | AT1G20310 | 28.731 | 268 | 136 | 11 | 1 | 243 | 1 | 238 | 3.05e-17 | 78.6 |
Find 47 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCCTTCTTCCCTCAGATTT+TGG | 0.122137 | 3.3:-93641965 | None:intergenic |
| ATCAATTCCATTAATATCTT+TGG | 0.179497 | 3.3:-93641804 | None:intergenic |
| TCTAGCTTCATGTAGAATTA+TGG | 0.199503 | 3.3:-93641774 | None:intergenic |
| CTTGGTGGTGGTGGTGTTGC+TGG | 0.256841 | 3.3:+93642217 | MS.gene69361:CDS |
| CCAAGTTTGATCTAATTTAC+TGG | 0.265351 | 3.3:-93642331 | None:intergenic |
| AGAAAGTCCAAAGATATTAA+TGG | 0.282367 | 3.3:+93641797 | MS.gene69361:CDS |
| AGATCTTGTTCCTTAGCTTA+AGG | 0.361689 | 3.3:-93641610 | None:intergenic |
| GGTGTTGCTGGTTGTAGTCT+TGG | 0.383396 | 3.3:+93642229 | MS.gene69361:CDS |
| AAAAGATGTTATTTCATGTC+GGG | 0.387650 | 3.3:+93641939 | MS.gene69361:CDS |
| TGTAGAATTATGGATGCAAT+TGG | 0.397830 | 3.3:-93641764 | None:intergenic |
| ATCCCTTTCTCTGCACCTCT+TGG | 0.403077 | 3.3:+93642199 | MS.gene69361:CDS |
| TCCACCTAGGCCTCTACAAC+TGG | 0.412969 | 3.3:+93642306 | MS.gene69361:CDS |
| CTTGATTTGTGATCCCTACC+TGG | 0.418438 | 3.3:-93641686 | None:intergenic |
| CCTTTCTCTGCACCTCTTGG+TGG | 0.430613 | 3.3:+93642202 | MS.gene69361:CDS |
| TCTGCACCTCTTGGTGGTGG+TGG | 0.444151 | 3.3:+93642208 | MS.gene69361:CDS |
| GGATCCAGTTGTAGAGGCCT+AGG | 0.476355 | 3.3:-93642310 | None:intergenic |
| TTCTCTGCACCTCTTGGTGG+TGG | 0.477822 | 3.3:+93642205 | MS.gene69361:CDS |
| TAGTAATTATCTATTTCCTC+TGG | 0.477991 | 3.3:-93642118 | None:intergenic |
| CACGTAAAGAAATTAATCTA+TGG | 0.479566 | 3.3:+93642266 | MS.gene69361:CDS |
| CCACCAAGAGGTGCAGAGAA+AGG | 0.489526 | 3.3:-93642202 | None:intergenic |
| AACCAAAATCTGAGGGAAGA+AGG | 0.495894 | 3.3:+93641963 | MS.gene69361:CDS |
| GACAAGATAACGCAACAAAG+TGG | 0.496463 | 3.3:+93641708 | MS.gene69361:CDS |
| TGATCCCTACCTGGACTGAA+TGG | 0.498481 | 3.3:-93641677 | None:intergenic |
| CACCAAGAGGTGCAGAGAAA+GGG | 0.499821 | 3.3:-93642201 | None:intergenic |
| CAAAAGATGTTATTTCATGT+CGG | 0.505412 | 3.3:+93641938 | MS.gene69361:CDS |
| AGATAAGAAGTATGCAATTG+AGG | 0.507400 | 3.3:+93642009 | MS.gene69361:CDS |
| CATAGATTAATTTCTTTACG+TGG | 0.529657 | 3.3:-93642265 | None:intergenic |
| GGCAAGACCTCATGGTGTCA+AGG | 0.540397 | 3.3:+93641730 | MS.gene69361:CDS |
| CAAAACCATCCATTCAGTCC+AGG | 0.540895 | 3.3:+93641668 | MS.gene69361:CDS |
| TGATGATGATGAAGAAGAAG+AGG | 0.542344 | 3.3:+93642168 | MS.gene69361:CDS |
| CCCTACCTGGACTGAATGGA+TGG | 0.542832 | 3.3:-93641673 | None:intergenic |
| AAATTAATCTATGGAAGAGA+AGG | 0.544809 | 3.3:+93642275 | MS.gene69361:CDS |
| CCATCCATTCAGTCCAGGTA+GGG | 0.567822 | 3.3:+93641673 | MS.gene69361:CDS |
| TAGAGGCCTAGGTGGAGCCA+TGG | 0.570574 | 3.3:-93642299 | None:intergenic |
| TTTACTGGATCCAGTTGTAG+AGG | 0.574544 | 3.3:-93642316 | None:intergenic |
| ACAAAGTGGGCAAGACCTCA+TGG | 0.574674 | 3.3:+93641722 | MS.gene69361:CDS |
| GCAACACCACCACCACCAAG+AGG | 0.576743 | 3.3:-93642214 | None:intergenic |
| GCAAGACCTCATGGTGTCAA+GGG | 0.587142 | 3.3:+93641731 | MS.gene69361:CDS |
| TGTCGGGAAACCAAAATCTG+AGG | 0.596722 | 3.3:+93641955 | MS.gene69361:CDS |
| ACAAGATAACGCAACAAAGT+GGG | 0.602580 | 3.3:+93641709 | MS.gene69361:CDS |
| GTCGGGAAACCAAAATCTGA+GGG | 0.603177 | 3.3:+93641956 | MS.gene69361:CDS |
| TCCAGTTGTAGAGGCCTAGG+TGG | 0.615336 | 3.3:-93642307 | None:intergenic |
| GAAGGACCATGGCTCCACCT+AGG | 0.627085 | 3.3:+93642293 | MS.gene69361:CDS |
| GAAGGCTCAGGTAGCACCAG+AGG | 0.628886 | 3.3:+93642102 | MS.gene69361:CDS |
| GAAAATCCCTTGACACCATG+AGG | 0.634828 | 3.3:-93641737 | None:intergenic |
| ACCATCCATTCAGTCCAGGT+AGG | 0.635976 | 3.3:+93641672 | MS.gene69361:CDS |
| TCTATGGAAGAGAAGGACCA+TGG | 0.674583 | 3.3:+93642282 | MS.gene69361:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCAATTCCATTAATATCTT+TGG | - | chr3.3:93641807-93641826 | None:intergenic | 20.0% |
| !!! | AAGTTTATAGTGATAGTTTT+TGG | - | chr3.3:93641647-93641666 | None:intergenic | 20.0% |
| ! | AAATTAATCTATGGAAGAGA+AGG | + | chr3.3:93642275-93642294 | MS.gene69361:CDS | 25.0% |
| ! | AGAAAGTCCAAAGATATTAA+TGG | + | chr3.3:93641797-93641816 | MS.gene69361:CDS | 25.0% |
| ! | CACGTAAAGAAATTAATCTA+TGG | + | chr3.3:93642266-93642285 | MS.gene69361:CDS | 25.0% |
| ! | CATAGATTAATTTCTTTACG+TGG | - | chr3.3:93642268-93642287 | None:intergenic | 25.0% |
| ! | TAGTAATTATCTATTTCCTC+TGG | - | chr3.3:93642121-93642140 | None:intergenic | 25.0% |
| !!! | AAAAGATGTTATTTCATGTC+GGG | + | chr3.3:93641939-93641958 | MS.gene69361:CDS | 25.0% |
| !!! | ACACTTTTGCTAATTTTGAT+TGG | + | chr3.3:93642080-93642099 | MS.gene69361:CDS | 25.0% |
| !!! | CAAAAGATGTTATTTCATGT+CGG | + | chr3.3:93641938-93641957 | MS.gene69361:CDS | 25.0% |
| !!! | TTTTGCTAATTTTGATTGGA+AGG | + | chr3.3:93642084-93642103 | MS.gene69361:CDS | 25.0% |
| TGTAGAATTATGGATGCAAT+TGG | - | chr3.3:93641767-93641786 | None:intergenic | 30.0% | |
| ! | AGATAAGAAGTATGCAATTG+AGG | + | chr3.3:93642009-93642028 | MS.gene69361:CDS | 30.0% |
| ! | TCTAGCTTCATGTAGAATTA+TGG | - | chr3.3:93641777-93641796 | None:intergenic | 30.0% |
| ACAAGATAACGCAACAAAGT+GGG | + | chr3.3:93641709-93641728 | MS.gene69361:CDS | 35.0% | |
| AGATCTTGTTCCTTAGCTTA+AGG | - | chr3.3:93641613-93641632 | None:intergenic | 35.0% | |
| TCACCAAAAATATCGTGCAT+GGG | + | chr3.3:93641842-93641861 | MS.gene69361:CDS | 35.0% | |
| TGGAGAAAAACCTTAAGCTA+AGG | + | chr3.3:93641600-93641619 | MS.gene69361:CDS | 35.0% | |
| TTCACCAAAAATATCGTGCA+TGG | + | chr3.3:93641841-93641860 | MS.gene69361:CDS | 35.0% | |
| ! | CAAATGAAACGTTTTGCAAG+TGG | + | chr3.3:93642052-93642071 | MS.gene69361:CDS | 35.0% |
| ! | TGATGATGATGAAGAAGAAG+AGG | + | chr3.3:93642168-93642187 | MS.gene69361:CDS | 35.0% |
| !!! | CACGATATTTTTGGTGAAGT+GGG | - | chr3.3:93641839-93641858 | None:intergenic | 35.0% |
| !!! | TAATTTTGATTGGAAGGCTC+AGG | + | chr3.3:93642090-93642109 | MS.gene69361:CDS | 35.0% |
| AACCAAAATCTGAGGGAAGA+AGG | + | chr3.3:93641963-93641982 | MS.gene69361:CDS | 40.0% | |
| GACAAGATAACGCAACAAAG+TGG | + | chr3.3:93641708-93641727 | MS.gene69361:CDS | 40.0% | |
| TTTACTGGATCCAGTTGTAG+AGG | - | chr3.3:93642319-93642338 | None:intergenic | 40.0% | |
| ! | TGTCCCATGCACGATATTTT+TGG | - | chr3.3:93641848-93641867 | None:intergenic | 40.0% |
| ! | TTCCTTCTTCCCTCAGATTT+TGG | - | chr3.3:93641968-93641987 | None:intergenic | 40.0% |
| !!! | GCACGATATTTTTGGTGAAG+TGG | - | chr3.3:93641840-93641859 | None:intergenic | 40.0% |
| CAAAACCATCCATTCAGTCC+AGG | + | chr3.3:93641668-93641687 | MS.gene69361:CDS | 45.0% | |
| GAAAATCCCTTGACACCATG+AGG | - | chr3.3:93641740-93641759 | None:intergenic | 45.0% | |
| GTCGGGAAACCAAAATCTGA+GGG | + | chr3.3:93641956-93641975 | MS.gene69361:CDS | 45.0% | |
| TCTATGGAAGAGAAGGACCA+TGG | + | chr3.3:93642282-93642301 | MS.gene69361:CDS | 45.0% | |
| TGTCGGGAAACCAAAATCTG+AGG | + | chr3.3:93641955-93641974 | MS.gene69361:CDS | 45.0% | |
| !! | CATGGTGTCAAGGGATTTTC+AGG | + | chr3.3:93641740-93641759 | MS.gene69361:CDS | 45.0% |
| !! | CTTGATTTGTGATCCCTACC+TGG | - | chr3.3:93641689-93641708 | None:intergenic | 45.0% |
| ACAAAGTGGGCAAGACCTCA+TGG | + | chr3.3:93641722-93641741 | MS.gene69361:CDS | 50.0% | |
| ACCATCCATTCAGTCCAGGT+AGG | + | chr3.3:93641672-93641691 | MS.gene69361:CDS | 50.0% | |
| ATCCCTTTCTCTGCACCTCT+TGG | + | chr3.3:93642199-93642218 | MS.gene69361:CDS | 50.0% | |
| CCATCCATTCAGTCCAGGTA+GGG | + | chr3.3:93641673-93641692 | MS.gene69361:CDS | 50.0% | |
| TGATCCCTACCTGGACTGAA+TGG | - | chr3.3:93641680-93641699 | None:intergenic | 50.0% | |
| ! | CACCAAGAGGTGCAGAGAAA+GGG | - | chr3.3:93642204-93642223 | None:intergenic | 50.0% |
| ! | GCAAGACCTCATGGTGTCAA+GGG | + | chr3.3:93641731-93641750 | MS.gene69361:CDS | 50.0% |
| !! | GGTGTTGCTGGTTGTAGTCT+TGG | + | chr3.3:93642229-93642248 | MS.gene69361:CDS | 50.0% |
| CCCTACCTGGACTGAATGGA+TGG | - | chr3.3:93641676-93641695 | None:intergenic | 55.0% | |
| CCTTTCTCTGCACCTCTTGG+TGG | + | chr3.3:93642202-93642221 | MS.gene69361:CDS | 55.0% | |
| GGATCCAGTTGTAGAGGCCT+AGG | - | chr3.3:93642313-93642332 | None:intergenic | 55.0% | |
| TCCACCTAGGCCTCTACAAC+TGG | + | chr3.3:93642306-93642325 | MS.gene69361:CDS | 55.0% | |
| TCCAGTTGTAGAGGCCTAGG+TGG | - | chr3.3:93642310-93642329 | None:intergenic | 55.0% | |
| TTCTCTGCACCTCTTGGTGG+TGG | + | chr3.3:93642205-93642224 | MS.gene69361:CDS | 55.0% | |
| ! | CCACCAAGAGGTGCAGAGAA+AGG | - | chr3.3:93642205-93642224 | None:intergenic | 55.0% |
| ! | GGCAAGACCTCATGGTGTCA+AGG | + | chr3.3:93641730-93641749 | MS.gene69361:CDS | 55.0% |
| GAAGGACCATGGCTCCACCT+AGG | + | chr3.3:93642293-93642312 | MS.gene69361:CDS | 60.0% | |
| GAAGGCTCAGGTAGCACCAG+AGG | + | chr3.3:93642102-93642121 | MS.gene69361:CDS | 60.0% | |
| GCAACACCACCACCACCAAG+AGG | - | chr3.3:93642217-93642236 | None:intergenic | 60.0% | |
| TAGAGGCCTAGGTGGAGCCA+TGG | - | chr3.3:93642302-93642321 | None:intergenic | 60.0% | |
| !! | CTTGGTGGTGGTGGTGTTGC+TGG | + | chr3.3:93642217-93642236 | MS.gene69361:CDS | 60.0% |
| !! | TCTGCACCTCTTGGTGGTGG+TGG | + | chr3.3:93642208-93642227 | MS.gene69361:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 93641599 | 93642342 | 93641599 | ID=MS.gene69361 |
| chr3.3 | mRNA | 93641599 | 93642342 | 93641599 | ID=MS.gene69361.t1;Parent=MS.gene69361 |
| chr3.3 | exon | 93641599 | 93642342 | 93641599 | ID=MS.gene69361.t1.exon1;Parent=MS.gene69361.t1 |
| chr3.3 | CDS | 93641599 | 93642342 | 93641599 | ID=cds.MS.gene69361.t1;Parent=MS.gene69361.t1 |
| Gene Sequence |
| Protein sequence |
