AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene69800

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene69800.t1 MTR_2g082640 98.696 230 2 1 1 229 1 230 4.39e-168 462
MS.gene69800.t1 MTR_4g081370 78.571 238 41 4 1 229 1 237 2.69e-125 354
MS.gene69800.t1 MTR_4g081370 87.634 186 22 1 45 229 8 193 3.65e-114 325
MS.gene69800.t1 MTR_2g089000 50.195 257 84 8 9 229 12 260 1.26e-72 222
MS.gene69800.t1 MTR_1g043430 52.273 176 80 1 54 229 75 246 6.18e-58 184
MS.gene69800.t1 MTR_4g075480 51.515 99 47 1 69 167 6 103 4.94e-23 94.4
MS.gene69800.t1 MTR_4g075480 51.515 99 47 1 69 167 34 131 1.28e-22 94.0
MS.gene69800.t1 MTR_1g032600 36.806 144 78 3 32 167 36 174 1.99e-19 85.9
MS.gene69800.t1 MTR_4g108360 33.898 118 74 2 51 168 35 148 4.39e-13 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene69800.t1 AT5G54680 69.787 235 64 5 1 229 1 234 2.18e-108 311
MS.gene69800.t1 AT1G51070 62.343 239 67 6 1 229 1 226 5.99e-95 277
MS.gene69800.t1 AT1G51070 65.829 199 57 4 36 229 100 292 3.08e-84 252
MS.gene69800.t1 AT4G14410 52.041 196 77 6 37 229 96 277 2.77e-55 178
MS.gene69800.t1 AT4G14410 52.041 196 77 6 37 229 96 277 2.77e-55 178
MS.gene69800.t1 AT4G14410 52.041 196 77 6 37 229 102 283 3.14e-55 178
MS.gene69800.t1 AT3G23210 52.261 199 87 5 35 229 97 291 4.98e-55 178
MS.gene69800.t1 AT3G23210 52.261 199 87 5 35 229 126 320 1.37e-54 177
MS.gene69800.t1 AT3G19860 45.299 117 59 2 66 178 2 117 1.24e-22 93.6
MS.gene69800.t1 AT3G19860 45.299 117 59 2 66 178 33 148 2.25e-22 93.6
MS.gene69800.t1 AT3G19860 45.299 117 59 2 66 178 55 170 2.39e-22 93.6
MS.gene69800.t1 AT4G36060 38.971 136 72 5 69 199 26 155 1.31e-18 82.4
MS.gene69800.t1 AT4G36060 39.706 136 71 5 69 199 26 155 2.32e-18 81.6
MS.gene69800.t1 AT4G36060 38.971 136 72 5 69 199 44 173 4.41e-18 81.3
MS.gene69800.t1 AT4G36060 39.706 136 71 5 69 199 44 173 4.76e-18 81.3
MS.gene69800.t1 AT3G47640 30.128 156 93 4 73 216 31 182 4.93e-12 63.9
MS.gene69800.t1 AT3G47640 30.128 156 93 4 73 216 31 182 4.93e-12 63.9
MS.gene69800.t1 AT3G47640 30.128 156 93 4 73 216 31 182 4.93e-12 63.9

Find 77 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGAAGATTAAAGAGTTAA+AGG 0.125284 2.4:-16583962 MS.gene69800:intron
AATTTGAGTCTTTCAACTTT+TGG 0.132451 2.4:+16583995 None:intergenic
TGAAAGGCATTAACTTGTTT+GGG 0.247330 2.4:+16583616 None:intergenic
ATGAAAGGCATTAACTTGTT+TGG 0.257284 2.4:+16583615 None:intergenic
ATTGATAGGTTTGTTGAATT+AGG 0.284956 2.4:-16584111 MS.gene69800:intron
AGGAGGGTGTGTCAAGAAAC+TGG 0.351738 2.4:+16583674 None:intergenic
ATGTGCTCCTTCCAGCTCTA+AGG 0.373277 2.4:-16584268 MS.gene69800:CDS
GCATTAACTTGTTTGGGGCT+TGG 0.383016 2.4:+16583622 None:intergenic
CTCTGCTCCTAGTATTGAAA+TGG 0.383024 2.4:-16584651 MS.gene69800:intron
GCAAATGCAGCAGGGATTGC+AGG 0.388118 2.4:+16583654 None:intergenic
AAGGAGCCATCGACAGAAAC+AGG 0.397378 2.4:+16585486 None:intergenic
CGGTTGCCAATTTAGGCAAC+TGG 0.406655 2.4:+16583513 None:intergenic
CCGAATGGTGACACAGTTGC+GGG 0.410732 2.4:-16584025 MS.gene69800:CDS
AAGTTGAAAGACTCAAATTC+AGG 0.412450 2.4:-16583991 MS.gene69800:CDS
TGTTGAATTAGGCTCCATCT+TGG 0.436518 2.4:-16584100 MS.gene69800:CDS
TAAGGCTGAGAAGGAGAAGT+TGG 0.437128 2.4:-16583727 MS.gene69800:CDS
GAAGGAGAAGTTGGAGCAGC+AGG 0.442475 2.4:-16583718 MS.gene69800:CDS
ATGCCTTTCATGAGTTATCC+AGG 0.449494 2.4:-16583603 MS.gene69800:CDS
AATTGCCACATGGCAACTCC+TGG 0.456430 2.4:+16583585 None:intergenic
CCCGCAACTGTGTCACCATT+CGG 0.471915 2.4:+16584025 None:intergenic
TGATGAGAAGCAGAGGCTTA+AGG 0.477872 2.4:-16583745 MS.gene69800:CDS
TTTCCCCGGAAAACACCAAT+TGG 0.488899 2.4:-16585539 MS.gene69800:CDS
GAGAGAAGTTGCGGAGGGAT+AGG 0.490114 2.4:-16584239 MS.gene69800:CDS
CCTTGATGAGCAAATGCAGC+AGG 0.490383 2.4:+16583645 None:intergenic
AGTTACCTCTTCTTCGAACC+AGG 0.496718 2.4:+16584590 None:intergenic
TTCTTGTTGCAGAGGTAGAT+CGG 0.503971 2.4:-16584295 MS.gene69800:intron
TCCGAATGGTGACACAGTTG+CGG 0.503977 2.4:-16584026 MS.gene69800:CDS
TTAGGCTCCATCTTGGAGCC+TGG 0.505934 2.4:-16584093 MS.gene69800:CDS
TGTGAGGTATCCACGGCGGC+TGG 0.507559 2.4:+16583555 None:intergenic
GAAATTCCTGTTTCTGTCGA+TGG 0.514909 2.4:-16585492 MS.gene69800:CDS
CAGGAATTTCATCAATCAAA+GGG 0.518186 2.4:+16585505 None:intergenic
GCCATTGGAGAGTTGAGGTG+GGG 0.518653 2.4:+16585449 None:intergenic
AGGGTAATCGAAAAGCCAAT+TGG 0.523695 2.4:+16585524 None:intergenic
CGCCATTGGAGAGTTGAGGT+GGG 0.524275 2.4:+16585448 None:intergenic
ACAGGAATTTCATCAATCAA+AGG 0.524589 2.4:+16585504 None:intergenic
CTCTCGACATGCCTTAGAGC+TGG 0.527575 2.4:+16584257 None:intergenic
CAACCCAACTATGGTTTCCC+CGG 0.528497 2.4:-16585553 None:intergenic
GCAGAGGCTTAAGGCTGAGA+AGG 0.531438 2.4:-16583736 MS.gene69800:CDS
CCTGCTGCATTTGCTCATCA+AGG 0.536277 2.4:-16583645 MS.gene69800:CDS
CACCTCAACTCTCCAATGGC+GGG 0.541239 2.4:-16585446 MS.gene69800:intron
GAGGTATCCACGGCGGCTGG+TGG 0.543817 2.4:+16583558 None:intergenic
CAAAATCAGATACCCGCCAT+TGG 0.547933 2.4:+16585434 None:intergenic
GTGTGTCAAGAAACTGGGTT+GGG 0.557971 2.4:+16583680 None:intergenic
GCTCCATCTTGGAGCCTGGA+AGG 0.560039 2.4:-16584089 MS.gene69800:CDS
AAGAACCTGGTTCGAAGAAG+AGG 0.561194 2.4:-16584595 MS.gene69800:intron
CCGCCATTGGAGAGTTGAGG+TGG 0.568621 2.4:+16585447 None:intergenic
GAAAGGCATTAACTTGTTTG+GGG 0.569345 2.4:+16583617 None:intergenic
CCATTGGAGAGTTGAGGTGG+GGG 0.574009 2.4:+16585450 None:intergenic
TGGCCTTCCAGGCTCCAAGA+TGG 0.580720 2.4:+16584086 None:intergenic
AATGGATGATAGCTCTCTAG+TGG 0.589069 2.4:-16584633 MS.gene69800:CDS
CGACATGCCTTAGAGCTGGA+AGG 0.592843 2.4:+16584261 None:intergenic
CAATTTAGGCAACTGGTGGA+CGG 0.594311 2.4:+16583520 None:intergenic
CCACCTCAACTCTCCAATGG+CGG 0.595417 2.4:-16585447 MS.gene69800:CDS
ATTCATGCCACCAGCCGCCG+TGG 0.596384 2.4:-16583565 MS.gene69800:CDS
AGGCATGTCGAGAGAAGTTG+CGG 0.604709 2.4:-16584248 MS.gene69800:CDS
ATGATCTTGTGAGGTATCCA+CGG 0.609314 2.4:+16583548 None:intergenic
ACTCCTGGATAACTCATGAA+AGG 0.613176 2.4:+16583600 None:intergenic
TCTGATGGTATCAAAGAACC+TGG 0.613566 2.4:-16584608 MS.gene69800:CDS
TTGCCAATTTAGGCAACTGG+TGG 0.613733 2.4:+16583516 None:intergenic
TACCCGCCATTGGAGAGTTG+AGG 0.621755 2.4:+16585444 None:intergenic
AACTTCGTGATGAGAAGCAG+AGG 0.627738 2.4:-16583752 MS.gene69800:CDS
GGTGTGTCAAGAAACTGGGT+TGG 0.631646 2.4:+16583679 None:intergenic
AGCTCTCTAGTGGATTCTGA+TGG 0.634077 2.4:-16584623 MS.gene69800:CDS
ATGGCTCCTTCGCCTTCACG+TGG 0.638692 2.4:-16585473 MS.gene69800:CDS
CTTGATGAGCAAATGCAGCA+GGG 0.642514 2.4:+16583646 None:intergenic
AATGCAGCAGGGATTGCAGG+AGG 0.644570 2.4:+16583657 None:intergenic
ATGCAGCAGGGATTGCAGGA+GGG 0.653807 2.4:+16583658 None:intergenic
CATGTCGAGAGAAGTTGCGG+AGG 0.656044 2.4:-16584245 MS.gene69800:CDS
ATGTCGAGAGAAGTTGCGGA+GGG 0.659997 2.4:-16584244 MS.gene69800:CDS
TCTGATTGATGCTGTCCGAA+TGG 0.662212 2.4:-16584040 MS.gene69800:CDS
TGGTGGCATGAATTGCCACA+TGG 0.684045 2.4:+16583575 None:intergenic
GGAGGGTGTGTCAAGAAACT+GGG 0.689856 2.4:+16583675 None:intergenic
ACGGAGTACATGATCTTGTG+AGG 0.697745 2.4:+16583539 None:intergenic
AAGGCCACCTAAAACAGACA+AGG 0.714338 2.4:-16584070 MS.gene69800:CDS
CGAATGGTGACACAGTTGCG+GGG 0.732614 2.4:-16584024 MS.gene69800:CDS
GTTATCCAGGAGTTGCCATG+TGG 0.743485 2.4:-16583590 MS.gene69800:CDS
ATCTTGTGAGGTATCCACGG+CGG 0.745646 2.4:+16583551 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAAAAAATACAAAAATAT+GGG + chr2.4:16584757-16584776 None:intergenic 10.0%
!! AACTTAGTAAATTAATACTA+TGG + chr2.4:16584379-16584398 None:intergenic 15.0%
!! ACTTAGTAAATTAATACTAT+GGG + chr2.4:16584378-16584397 None:intergenic 15.0%
!! AGTTAAAGTATAAAAATGAA+GGG + chr2.4:16585201-16585220 None:intergenic 15.0%
!! CAAGAAAAAATACAAAAATA+TGG + chr2.4:16584758-16584777 None:intergenic 15.0%
!!! AAAGTTTGAATTTTTAAACT+TGG - chr2.4:16583730-16583749 MS.gene69800:CDS 15.0%
!!! AAATGAAATGATTTAGAATA+TGG + chr2.4:16585178-16585197 None:intergenic 15.0%
!!! AAGTTTGAATTTTTAAACTT+GGG - chr2.4:16583731-16583750 MS.gene69800:CDS 15.0%
!! CAGTTAAAGTATAAAAATGA+AGG + chr2.4:16585202-16585221 None:intergenic 20.0%
!! GTAATATAATTGAGTCAAAT+TGG - chr2.4:16584614-16584633 MS.gene69800:CDS 20.0%
!! TATCCACAAATTTGAAAAAA+GGG + chr2.4:16583693-16583712 None:intergenic 20.0%
!! TCAATATTCAGTGTTATATA+AGG + chr2.4:16585264-16585283 None:intergenic 20.0%
!! TTATCCACAAATTTGAAAAA+AGG + chr2.4:16583694-16583713 None:intergenic 20.0%
!!! GTAGTTTTTATAAATTGTTG+TGG - chr2.4:16583778-16583797 MS.gene69800:intron 20.0%
! AAACCCTAAATTAAGAAAAG+GGG + chr2.4:16583659-16583678 None:intergenic 25.0%
! AATAATAAAAGGCAAGATAG+TGG + chr2.4:16584872-16584891 None:intergenic 25.0%
! AGAGAAGATTAAAGAGTTAA+AGG - chr2.4:16585105-16585124 MS.gene69800:intron 25.0%
! AGATTAAAGAGTTAAAGGTA+AGG - chr2.4:16585110-16585129 MS.gene69800:intron 25.0%
! AGTAAATTAATACTATGGGA+TGG + chr2.4:16584374-16584393 None:intergenic 25.0%
! ATAATAAAAGGCAAGATAGT+GGG + chr2.4:16584871-16584890 None:intergenic 25.0%
! ATCCACAAATTTGAAAAAAG+GGG + chr2.4:16583692-16583711 None:intergenic 25.0%
! CTAATATGATTGACATTGAT+AGG - chr2.4:16584942-16584961 MS.gene69800:intron 25.0%
! CTTGTTTAATGAGAAATGAA+AGG + chr2.4:16584514-16584533 None:intergenic 25.0%
! GAAACCCTAAATTAAGAAAA+GGG + chr2.4:16583660-16583679 None:intergenic 25.0%
! GTAAATTAATACTATGGGAT+GGG + chr2.4:16584373-16584392 None:intergenic 25.0%
! TCTTATAGTATACAAGCAAT+AGG + chr2.4:16584725-16584744 None:intergenic 25.0%
! TGAAACCCTAAATTAAGAAA+AGG + chr2.4:16583661-16583680 None:intergenic 25.0%
!! AAATTGCTCTAAATCAATTG+AGG + chr2.4:16585136-16585155 None:intergenic 25.0%
!! AAATTGTTGTGGTTTTCATT+TGG - chr2.4:16583789-16583808 MS.gene69800:intron 25.0%
!! AATTTGAGTCTTTCAACTTT+TGG + chr2.4:16585075-16585094 None:intergenic 25.0%
!! ATTTGAGTCTTTCAACTTTT+GGG + chr2.4:16585074-16585093 None:intergenic 25.0%
!! GACAAGTGTAATTTTTGAAT+TGG - chr2.4:16583896-16583915 MS.gene69800:intron 25.0%
!! TGTTATGTTTTGTGTACTTA+TGG - chr2.4:16583824-16583843 MS.gene69800:intron 25.0%
!!! AATTTTCTTTGAGAGATAGA+AGG - chr2.4:16585151-16585170 MS.gene69800:intron 25.0%
!!! ATTGATAGGTTTGTTGAATT+AGG - chr2.4:16584956-16584975 MS.gene69800:intron 25.0%
AAACCAAAACCTAAAATCCA+AGG + chr2.4:16584313-16584332 None:intergenic 30.0%
AAACCTAAAATCCAAGGTAA+AGG + chr2.4:16584307-16584326 None:intergenic 30.0%
AAAGGAATTGCAACAGTAAT+TGG + chr2.4:16584289-16584308 None:intergenic 30.0%
AAGAGGAGAGGAATAATAAA+AGG + chr2.4:16584883-16584902 None:intergenic 30.0%
AAGGAATTGCAACAGTAATT+GGG + chr2.4:16584288-16584307 None:intergenic 30.0%
AAGTTGAAAGACTCAAATTC+AGG - chr2.4:16585076-16585095 MS.gene69800:intron 30.0%
AATTGATATGTGTTAAACGC+TGG - chr2.4:16584105-16584124 MS.gene69800:CDS 30.0%
ACAGGAATTTCATCAATCAA+AGG + chr2.4:16583566-16583585 None:intergenic 30.0%
CAGGAATTTCATCAATCAAA+GGG + chr2.4:16583565-16583584 None:intergenic 30.0%
CTATCATCCATTTCAATACT+AGG + chr2.4:16584426-16584445 None:intergenic 30.0%
GTCAAATTGGCAATTTAACA+TGG - chr2.4:16584627-16584646 MS.gene69800:CDS 30.0%
TAATAAAAGGCAAGATAGTG+GGG + chr2.4:16584870-16584889 None:intergenic 30.0%
TTGAATTCTATCTACTATGC+AGG - chr2.4:16585280-16585299 MS.gene69800:intron 30.0%
TTTGTAGTAGAACTTGAACT+AGG - chr2.4:16583857-16583876 MS.gene69800:intron 30.0%
! ATTCCTTTACCTTGGATTTT+AGG - chr2.4:16584301-16584320 MS.gene69800:intron 30.0%
! GTTTTGTGTACTTATGGTTA+TGG - chr2.4:16583830-16583849 MS.gene69800:intron 30.0%
! TTGCCCCTTTTCTTAATTTA+GGG - chr2.4:16583653-16583672 MS.gene69800:CDS 30.0%
! TTTGCCCCTTTTCTTAATTT+AGG - chr2.4:16583652-16583671 MS.gene69800:CDS 30.0%
! TTTTTATTGATGCCACTGAA+CGG + chr2.4:16584687-16584706 None:intergenic 30.0%
!! ATGAAAGGCATTAACTTGTT+TGG + chr2.4:16585455-16585474 None:intergenic 30.0%
!! CCTAGTTTTCCATAATACAA+GGG + chr2.4:16584203-16584222 None:intergenic 30.0%
!! TCCTAGTTTTCCATAATACA+AGG + chr2.4:16584204-16584223 None:intergenic 30.0%
!! TGAAAGGCATTAACTTGTTT+GGG + chr2.4:16585454-16585473 None:intergenic 30.0%
!!! TGTATTTTTTCTTGTTGCAG+AGG - chr2.4:16584764-16584783 MS.gene69800:intron 30.0%
!!! TTACCTTGGATTTTAGGTTT+TGG - chr2.4:16584307-16584326 MS.gene69800:intron 30.0%
ACAAGCAATAGGATTTGACA+GGG + chr2.4:16584714-16584733 None:intergenic 35.0%
AGGAATTGCAACAGTAATTG+GGG + chr2.4:16584287-16584306 None:intergenic 35.0%
CCCTTGTATTATGGAAAACT+AGG - chr2.4:16584200-16584219 MS.gene69800:intron 35.0%
TACAAGCAATAGGATTTGAC+AGG + chr2.4:16584715-16584734 None:intergenic 35.0%
TAGTAGAACTTGAACTAGGA+TGG - chr2.4:16583861-16583880 MS.gene69800:intron 35.0%
TCAACTAACTTACAGCTTTG+AGG - chr2.4:16584592-16584611 MS.gene69800:intron 35.0%
TGAAGATAATTAGCGATAGC+AGG - chr2.4:16584659-16584678 MS.gene69800:intron 35.0%
TGTTCTTGAATCTTCTGTTC+TGG - chr2.4:16583929-16583948 MS.gene69800:intron 35.0%
TTGTGTACTTATGGTTATGG+TGG - chr2.4:16583833-16583852 MS.gene69800:intron 35.0%
! ATCTTCTGTTCTGGATATGT+TGG - chr2.4:16583938-16583957 MS.gene69800:intron 35.0%
! TATTCCTCTCCTCTTTTACT+CGG - chr2.4:16584888-16584907 MS.gene69800:intron 35.0%
!! AAAAGCCAATTGGTGTTTTC+CGG + chr2.4:16583536-16583555 None:intergenic 35.0%
!! GAAAGGCATTAACTTGTTTG+GGG + chr2.4:16585453-16585472 None:intergenic 35.0%
!!! TGCCCCTTTTTTCAAATTTG+TGG - chr2.4:16583687-16583706 MS.gene69800:CDS 35.0%
AACCTGCATCCCTTGTATTA+TGG - chr2.4:16584191-16584210 MS.gene69800:intron 40.0%
AATGGATGATAGCTCTCTAG+TGG - chr2.4:16584434-16584453 MS.gene69800:intron 40.0%
ACATACACGAACACAAGACA+CGG - chr2.4:16584015-16584034 MS.gene69800:CDS 40.0%
ACTCCTGGATAACTCATGAA+AGG + chr2.4:16585470-16585489 None:intergenic 40.0%
AGGGTAATCGAAAAGCCAAT+TGG + chr2.4:16583546-16583565 None:intergenic 40.0%
ATGCCTTTCATGAGTTATCC+AGG - chr2.4:16585464-16585483 MS.gene69800:CDS 40.0%
CTGTTGCAATTCCTTTACCT+TGG - chr2.4:16584293-16584312 MS.gene69800:intron 40.0%
TCTGATGGTATCAAAGAACC+TGG - chr2.4:16584459-16584478 MS.gene69800:intron 40.0%
TGGGTGTAGACTGTGTATTT+GGG - chr2.4:16584911-16584930 MS.gene69800:intron 40.0%
TGTTGAATTAGGCTCCATCT+TGG - chr2.4:16584967-16584986 MS.gene69800:intron 40.0%
TTCCATAATACAAGGGATGC+AGG + chr2.4:16584196-16584215 None:intergenic 40.0%
TTCTTGTTGCAGAGGTAGAT+CGG - chr2.4:16584772-16584791 MS.gene69800:intron 40.0%
! ATGATCTTGTGAGGTATCCA+CGG + chr2.4:16585522-16585541 None:intergenic 40.0%
! ATTGCTGCCTTGTCTGTTTT+AGG + chr2.4:16585007-16585026 None:intergenic 40.0%
! CTCTGCTCCTAGTATTGAAA+TGG - chr2.4:16584416-16584435 MS.gene69800:intron 40.0%
! TCTGTTCTGGATATGTTGGT+GGG - chr2.4:16583942-16583961 MS.gene69800:intron 40.0%
! TTCTGTTCTGGATATGTTGG+TGG - chr2.4:16583941-16583960 MS.gene69800:intron 40.0%
!! AAAGCCAATTGGTGTTTTCC+GGG + chr2.4:16583535-16583554 None:intergenic 40.0%
!! GAAATTCCTGTTTCTGTCGA+TGG - chr2.4:16583575-16583594 MS.gene69800:CDS 40.0%
AACTTCGTGATGAGAAGCAG+AGG - chr2.4:16585315-16585334 MS.gene69800:intron 45.0%
AAGGCCACCTAAAACAGACA+AGG - chr2.4:16584997-16585016 MS.gene69800:intron 45.0%
ACACAGCATGTGTCAGTGTT+GGG - chr2.4:16584082-16584101 MS.gene69800:CDS 45.0%
ACGGAGTACATGATCTTGTG+AGG + chr2.4:16585531-16585550 None:intergenic 45.0%
AGTTACCTCTTCTTCGAACC+AGG + chr2.4:16584480-16584499 None:intergenic 45.0%
CAAAATCAGATACCCGCCAT+TGG + chr2.4:16583636-16583655 None:intergenic 45.0%
CAATTTGCACGAGCAGCTTA+AGG + chr2.4:16584169-16584188 None:intergenic 45.0%
CTTGATGAGCAAATGCAGCA+GGG + chr2.4:16585424-16585443 None:intergenic 45.0%
GGGTGTAGACTGTGTATTTG+GGG - chr2.4:16584912-16584931 MS.gene69800:intron 45.0%
GTGGGTGTAGACTGTGTATT+TGG - chr2.4:16584910-16584929 MS.gene69800:intron 45.0%
GTGTGTCAAGAAACTGGGTT+GGG + chr2.4:16585390-16585409 None:intergenic 45.0%
TAAGGCTGAGAAGGAGAAGT+TGG - chr2.4:16585340-16585359 MS.gene69800:intron 45.0%
TCTGATTGATGCTGTCCGAA+TGG - chr2.4:16585027-16585046 MS.gene69800:intron 45.0%
TGATGAGAAGCAGAGGCTTA+AGG - chr2.4:16585322-16585341 MS.gene69800:intron 45.0%
TTTCCCCGGAAAACACCAAT+TGG - chr2.4:16583528-16583547 MS.gene69800:CDS 45.0%
! AAGAACCTGGTTCGAAGAAG+AGG - chr2.4:16584472-16584491 MS.gene69800:intron 45.0%
! CTGAAGTGTCAGTGCTACAT+AGG - chr2.4:16584141-16584160 MS.gene69800:intron 45.0%
!! AAGCCAATTGGTGTTTTCCG+GGG + chr2.4:16583534-16583553 None:intergenic 45.0%
!! AGCTCTCTAGTGGATTCTGA+TGG - chr2.4:16584444-16584463 MS.gene69800:intron 45.0%
!! GCATTAACTTGTTTGGGGCT+TGG + chr2.4:16585448-16585467 None:intergenic 45.0%
AAGGAGCCATCGACAGAAAC+AGG + chr2.4:16583584-16583603 None:intergenic 50.0%
AATTGCCACATGGCAACTCC+TGG + chr2.4:16585485-16585504 None:intergenic 50.0%
AGGCATGTCGAGAGAAGTTG+CGG - chr2.4:16584819-16584838 MS.gene69800:intron 50.0%
ATGTCGAGAGAAGTTGCGGA+GGG - chr2.4:16584823-16584842 MS.gene69800:intron 50.0%
ATGTGCTCCTTCCAGCTCTA+AGG - chr2.4:16584799-16584818 MS.gene69800:intron 50.0%
CCTGCTGCATTTGCTCATCA+AGG - chr2.4:16585422-16585441 MS.gene69800:intron 50.0%
CCTTGATGAGCAAATGCAGC+AGG + chr2.4:16585425-16585444 None:intergenic 50.0%
CTACACCCACCGAGTAAAAG+AGG + chr2.4:16584900-16584919 None:intergenic 50.0%
GACACAGCATGTGTCAGTGT+TGG - chr2.4:16584081-16584100 MS.gene69800:CDS 50.0%
GGTGTGTCAAGAAACTGGGT+TGG + chr2.4:16585391-16585410 None:intergenic 50.0%
GTTATCCAGGAGTTGCCATG+TGG - chr2.4:16585477-16585496 MS.gene69800:CDS 50.0%
TCCGAATGGTGACACAGTTG+CGG - chr2.4:16585041-16585060 MS.gene69800:intron 50.0%
TGGTGGCATGAATTGCCACA+TGG + chr2.4:16585495-16585514 None:intergenic 50.0%
! AGGAGGGTGTGTCAAGAAAC+TGG + chr2.4:16585396-16585415 None:intergenic 50.0%
! ATCTTGTGAGGTATCCACGG+CGG + chr2.4:16585519-16585538 None:intergenic 50.0%
! CCTCTCCTCTTTTACTCGGT+GGG - chr2.4:16584892-16584911 MS.gene69800:intron 50.0%
! GGAGGGTGTGTCAAGAAACT+GGG + chr2.4:16585395-16585414 None:intergenic 50.0%
! TCCTCTCCTCTTTTACTCGG+TGG - chr2.4:16584891-16584910 MS.gene69800:intron 50.0%
!! GCTGCCTTGTCTGTTTTAGG+TGG + chr2.4:16585004-16585023 None:intergenic 50.0%
!! TCTGTTTTAGGTGGCCTTCC+AGG + chr2.4:16584995-16585014 None:intergenic 50.0%
AATGCAGCAGGGATTGCAGG+AGG + chr2.4:16585413-16585432 None:intergenic 55.0%
ATGCAGCAGGGATTGCAGGA+GGG + chr2.4:16585412-16585431 None:intergenic 55.0%
CACCTCAACTCTCCAATGGC+GGG - chr2.4:16583621-16583640 MS.gene69800:CDS 55.0%
CATGTCGAGAGAAGTTGCGG+AGG - chr2.4:16584822-16584841 MS.gene69800:intron 55.0%
CCACCTCAACTCTCCAATGG+CGG - chr2.4:16583620-16583639 MS.gene69800:CDS 55.0%
CCATTGGAGAGTTGAGGTGG+GGG + chr2.4:16583620-16583639 None:intergenic 55.0%
CCCACCGAGTAAAAGAGGAG+AGG + chr2.4:16584895-16584914 None:intergenic 55.0%
CCCGCAACTGTGTCACCATT+CGG + chr2.4:16585045-16585064 None:intergenic 55.0%
CCGAATGGTGACACAGTTGC+GGG - chr2.4:16585042-16585061 MS.gene69800:intron 55.0%
CGAATGGTGACACAGTTGCG+GGG - chr2.4:16585043-16585062 MS.gene69800:intron 55.0%
CGACATGCCTTAGAGCTGGA+AGG + chr2.4:16584809-16584828 None:intergenic 55.0%
CGCCATTGGAGAGTTGAGGT+GGG + chr2.4:16583622-16583641 None:intergenic 55.0%
CTCTCGACATGCCTTAGAGC+TGG + chr2.4:16584813-16584832 None:intergenic 55.0%
GAAGGAGAAGTTGGAGCAGC+AGG - chr2.4:16585349-16585368 MS.gene69800:intron 55.0%
GAGAGAAGTTGCGGAGGGAT+AGG - chr2.4:16584828-16584847 MS.gene69800:intron 55.0%
GCAAATGCAGCAGGGATTGC+AGG + chr2.4:16585416-16585435 None:intergenic 55.0%
GCAGAGGCTTAAGGCTGAGA+AGG - chr2.4:16585331-16585350 MS.gene69800:intron 55.0%
GCCATTGGAGAGTTGAGGTG+GGG + chr2.4:16583621-16583640 None:intergenic 55.0%
TACCCGCCATTGGAGAGTTG+AGG + chr2.4:16583626-16583645 None:intergenic 55.0%
TTAGGCTCCATCTTGGAGCC+TGG - chr2.4:16584974-16584993 MS.gene69800:intron 55.0%
ATGGCTCCTTCGCCTTCACG+TGG - chr2.4:16583594-16583613 MS.gene69800:CDS 60.0%
CCCCCACCTCAACTCTCCAA+TGG - chr2.4:16583617-16583636 MS.gene69800:CDS 60.0%
CCGCCATTGGAGAGTTGAGG+TGG + chr2.4:16583623-16583642 None:intergenic 60.0%
CGATAGCAGGCTCCGTTCAG+TGG - chr2.4:16584672-16584691 MS.gene69800:intron 60.0%
GCTCCATCTTGGAGCCTGGA+AGG - chr2.4:16584978-16584997 MS.gene69800:intron 60.0%
TGGCCTTCCAGGCTCCAAGA+TGG + chr2.4:16584984-16585003 None:intergenic 60.0%
ATTCATGCCACCAGCCGCCG+TGG - chr2.4:16585502-16585521 MS.gene69800:CDS 65.0%
TTGAGGTGGGGGCCACGTGA+AGG + chr2.4:16583609-16583628 None:intergenic 65.0%
! TGTGAGGTATCCACGGCGGC+TGG + chr2.4:16585515-16585534 None:intergenic 65.0%
TGGGGGCCACGTGAAGGCGA+AGG + chr2.4:16583603-16583622 None:intergenic 70.0%
! GAGGTATCCACGGCGGCTGG+TGG + chr2.4:16585512-16585531 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 16583524 16585565 16583524 ID=MS.gene69800
chr2.4 mRNA 16583524 16585565 16583524 ID=MS.gene69800.t1;Parent=MS.gene69800
chr2.4 exon 16585447 16585565 16585447 ID=MS.gene69800.t1.exon1;Parent=MS.gene69800.t1
chr2.4 CDS 16585447 16585565 16585447 ID=cds.MS.gene69800.t1;Parent=MS.gene69800.t1
chr2.4 exon 16584596 16584661 16584596 ID=MS.gene69800.t1.exon2;Parent=MS.gene69800.t1
chr2.4 CDS 16584596 16584661 16584596 ID=cds.MS.gene69800.t1;Parent=MS.gene69800.t1
chr2.4 exon 16584228 16584305 16584228 ID=MS.gene69800.t1.exon3;Parent=MS.gene69800.t1
chr2.4 CDS 16584228 16584305 16584228 ID=cds.MS.gene69800.t1;Parent=MS.gene69800.t1
chr2.4 exon 16583963 16584125 16583963 ID=MS.gene69800.t1.exon4;Parent=MS.gene69800.t1
chr2.4 CDS 16583963 16584125 16583963 ID=cds.MS.gene69800.t1;Parent=MS.gene69800.t1
chr2.4 exon 16583524 16583787 16583524 ID=MS.gene69800.t1.exon5;Parent=MS.gene69800.t1
chr2.4 CDS 16583524 16583787 16583524 ID=cds.MS.gene69800.t1;Parent=MS.gene69800.t1
Gene Sequence

>MS.gene69800

ATGGTTTCCCCGGAAAACACCAATTGGCTTTTCGATTACCCTTTGATTGATGAAATTCCTGTTTCTGTCGATGGCTCCTTCGCCTTCACGTGGCCCCCACCTCAACTCTCCAATGGCGGGTATCTGATTTTGCCCCTTTTCTTAATTTAGGGTTTCAAGTTTTTGCCCCTTTTTTCAAATTTGTGGATAATTGTTGTGCTTTTTGTAAAGTTTGAATTTTTAAACTTGGGTTTGTAAAATTTTGTTGTAAAATTGTAGTTTTTATAAATTGTTGTGGTTTTCATTTGGTCTTCAGTTATTTGTTATGTTTTGTGTACTTATGGTTATGGTGGATTTGTAGTAGAACTTGAACTAGGATGGAAGTACAAGTGTGACAAGTGTAATTTTTGAATTGGTTAATGATGATGTTCTTGAATCTTCTGTTCTGGATATGTTGGTGGGTTTAATTGTCTGTGCTGTCTTGTTTCAATAGCTGTTGATGAAGCATCGACACATACACGAACACAAGACACGGCACTGACACTAGTAAACAATTTGAGAAAAATGAATAATTAAATGACACAGCATGTGTCAGTGTTGGGAATTGATATGTGTTAAACGCTGGAAACACTTTCAATCTGAAGTGTCAGTGCTACATAGGTGCCTTAAGCTGCTCGTGCAAATTGACAACCTGCATCCCTTGTATTATGGAAAACTAGGAACTAATTGTGACTGATGCAATCGTAGTTTAATTACAACAGCGATGATGTAGTTGTAGAGACCCCAATTACTGTTGCAATTCCTTTACCTTGGATTTTAGGTTTTGGTTTGAGAATTAGAATTTTTTTCTTCTATAAATACATATCTCCCATCCCATAGTATTAATTTACTAAGTTATGCTTCTGTGTTATCTCTCTGCTCCTAGTATTGAAATGGATGATAGCTCTCTAGTGGATTCTGATGGTATCAAAGAACCTGGTTCGAAGAAGAGGTAACTATTTTGTTTTACCTTTCATTTCTCATTAAACAAGTTAGTCAATGTTAATAAAATCAACACATGATCGACATGAGTAGTGTAGCGACTTAGCGTCAACTAACTTACAGCTTTGAGGTAATATAATTGAGTCAAATTGGCAATTTAACATGGTTTCTAAAGTGAAGATAATTAGCGATAGCAGGCTCCGTTCAGTGGCATCAATAAAAAAATTCCCTGTCAAATCCTATTGCTTGTATACTATAAGAAAAAGTTATCCCATATTTTTGTATTTTTTCTTGTTGCAGAGGTAGATCGGATTCATGTGCTCCTTCCAGCTCTAAGGCATGTCGAGAGAAGTTGCGGAGGGATAGGCTGAACGACAAGTAATCCCCACTATCTTGCCTTTTATTATTCCTCTCCTCTTTTACTCGGTGGGTGTAGACTGTGTATTTGGGGTATGCAACTAATATGATTGACATTGATAGGTTTGTTGAATTAGGCTCCATCTTGGAGCCTGGAAGGCCACCTAAAACAGACAAGGCAGCAATTCTGATTGATGCTGTCCGAATGGTGACACAGTTGCGGGGTGAAGCCCAAAAGTTGAAAGACTCAAATTCAGGTCTTCAAGAGAAGATTAAAGAGTTAAAGGTAAGGCCTCAATTGATTTAGAGCAATTTTCTTTGAGAGATAGAAGGTCCATATTCTAAATCATTTCATTTCCCTTCATTTTTATACTTTAACTGTAGTACTTTGTCATATCAGTGACTTCAATATCTGATTGACCTTATATAACACTGAATATTGAATTCTATCTACTATGCAGGTTGAGAAGAATGAACTTCGTGATGAGAAGCAGAGGCTTAAGGCTGAGAAGGAGAAGTTGGAGCAGCAGGTGAAATCAATGAACACCCAACCCAGTTTCTTGACACACCCTCCTGCAATCCCTGCTGCATTTGCTCATCAAGGCCAAGCCCCAAACAAGTTAATGCCTTTCATGAGTTATCCAGGAGTTGCCATGTGGCAATTCATGCCACCAGCCGCCGTGGATACCTCACAAGATCATGTACTCCGTCCACCAGTTGCCTAA
Protein sequence

>MS.gene69800.t1

MVSPENTNWLFDYPLIDEIPVSVDGSFAFTWPPPQLSNGGIEMDDSSLVDSDGIKEPGSKKRGRSDSCAPSSSKACREKLRRDRLNDKFVELGSILEPGRPPKTDKAAILIDAVRMVTQLRGEAQKLKDSNSGLQEKIKELKVEKNELRDEKQRLKAEKEKLEQQVKSMNTQPSFLTHPPAIPAAFAHQGQAPNKLMPFMSYPGVAMWQFMPPAAVDTSQDHVLRPPVA