Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69855.t1 | XP_003596709.2 | 91.4 | 233 | 20 | 0 | 1 | 233 | 1 | 233 | 2.60E-112 | 414.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69855.t1 | G7IM74 | 91.4 | 233 | 20 | 0 | 1 | 233 | 1 | 233 | 1.8e-112 | 414.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056677 | MS.gene69855 | 0.815399 | 9.43E-52 | -1.69E-46 |
| MS.gene05952 | MS.gene69855 | 0.810375 | 1.19E-50 | -1.69E-46 |
| MS.gene060012 | MS.gene69855 | 0.80472 | 1.89E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69855.t1 | MTR_2g083910 | 96.996 | 233 | 7 | 0 | 1 | 233 | 1 | 233 | 5.07e-165 | 455 |
| MS.gene69855.t1 | MTR_2g083910 | 96.970 | 231 | 7 | 0 | 1 | 231 | 1 | 231 | 7.26e-163 | 451 |
| MS.gene69855.t1 | MTR_4g080340 | 50.000 | 260 | 82 | 6 | 1 | 233 | 1 | 239 | 4.10e-63 | 197 |
| MS.gene69855.t1 | MTR_8g086820 | 45.382 | 249 | 113 | 7 | 1 | 233 | 1 | 242 | 1.14e-55 | 178 |
| MS.gene69855.t1 | MTR_2g016760 | 44.167 | 240 | 116 | 5 | 1 | 232 | 1 | 230 | 1.27e-53 | 177 |
| MS.gene69855.t1 | MTR_2g078620 | 47.059 | 102 | 42 | 5 | 134 | 230 | 225 | 319 | 1.70e-18 | 82.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69855.t1 | AT2G42760 | 37.363 | 273 | 124 | 9 | 1 | 233 | 1 | 266 | 2.96e-36 | 129 |
Find 38 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTATCAGAGCTTGAGTTTA+AGG | 0.133074 | 2.2:-13668151 | MS.gene69855:CDS |
| TAAGGAGCTAAAAGGGTTTA+TGG | 0.192552 | 2.2:-13668133 | MS.gene69855:CDS |
| CTTGAGTTTAAGGAGCTAAA+AGG | 0.195416 | 2.2:-13668141 | MS.gene69855:CDS |
| TTGTTAATCCATTGCTTAAT+TGG | 0.205608 | 2.2:-13667960 | MS.gene69855:CDS |
| GCTAAAAGGGTTTATGGATT+TGG | 0.214083 | 2.2:-13668127 | MS.gene69855:CDS |
| TGAAAGATAATCTTAAGTTT+TGG | 0.252639 | 2.2:-13667906 | MS.gene69855:CDS |
| CTAAAAGGGTTTATGGATTT+GGG | 0.263401 | 2.2:-13668126 | MS.gene69855:CDS |
| GCCTTATTTATCTGAGGCTT+GGG | 0.273923 | 2.2:-13668004 | MS.gene69855:CDS |
| TCTGAGGAAGATAAAGATTC+AGG | 0.292013 | 2.2:-13668093 | MS.gene69855:CDS |
| ACTTCTTTGCCTGAGAGAAT+TGG | 0.350310 | 2.2:+13668273 | None:intergenic |
| GGCCTTATTTATCTGAGGCT+TGG | 0.364492 | 2.2:-13668005 | MS.gene69855:CDS |
| GAATTCCAACACTTCAAGTT+AGG | 0.372690 | 2.2:-13668386 | MS.gene69855:CDS |
| CCCCAAGCCTCAGATAAATA+AGG | 0.382221 | 2.2:+13668003 | None:intergenic |
| TGAAACTGTTTGATATCAAC+TGG | 0.384594 | 2.2:-13668536 | MS.gene69855:CDS |
| GAAAACTTAGGCACGAAGAT+TGG | 0.434650 | 2.2:-13668351 | MS.gene69855:CDS |
| GGATTGGTTTCTTTGATACC+AGG | 0.467028 | 2.2:-13668072 | MS.gene69855:CDS |
| TGTGATGTGAGGACAGAGTT+TGG | 0.472758 | 2.2:+13668306 | None:intergenic |
| TTGAGTTTAAGGAGCTAAAA+GGG | 0.477612 | 2.2:-13668140 | MS.gene69855:CDS |
| AATTGGAGGGTACCAACTCT+TGG | 0.482818 | 2.2:-13667943 | MS.gene69855:CDS |
| AAGCTGAAGCCAATTCTCTC+AGG | 0.492170 | 2.2:-13668282 | MS.gene69855:CDS |
| TTCTTCCACTTGAGACATAG+AGG | 0.502500 | 2.2:+13668437 | None:intergenic |
| CACTTTCATCAACATTGTGT+TGG | 0.505818 | 2.2:+13668037 | None:intergenic |
| AAAGACCTAACTTGAAGTGT+TGG | 0.513872 | 2.2:+13668381 | None:intergenic |
| TTAATCCATTGCTTAATTGG+AGG | 0.514757 | 2.2:-13667957 | MS.gene69855:CDS |
| TTGATGAAAGTGTGATTAGT+AGG | 0.517023 | 2.2:-13668026 | MS.gene69855:CDS |
| GGAAGATAAAGATTCAGGAT+TGG | 0.523132 | 2.2:-13668088 | MS.gene69855:CDS |
| TAATCCATTGCTTAATTGGA+GGG | 0.524602 | 2.2:-13667956 | MS.gene69855:CDS |
| CATAGAACGAGACTTAGAAA+AGG | 0.547413 | 2.2:-13668192 | MS.gene69855:CDS |
| TAGTAGGCCTTATTTATCTG+AGG | 0.548733 | 2.2:-13668010 | MS.gene69855:CDS |
| CCTTATTTATCTGAGGCTTG+GGG | 0.550864 | 2.2:-13668003 | MS.gene69855:CDS |
| TCAATCTCATTGCCAAGAGT+TGG | 0.554919 | 2.2:+13667931 | None:intergenic |
| TTCAACCTCTATGTCTCAAG+TGG | 0.558037 | 2.2:-13668442 | MS.gene69855:CDS |
| AGTAATGTTGTTGAATGTAA+AGG | 0.562510 | 2.2:+13668471 | None:intergenic |
| GATTGGTTTCTTTGATACCA+GGG | 0.577636 | 2.2:-13668071 | MS.gene69855:CDS |
| TGTTGATTGTTATCTTACAA+TGG | 0.583010 | 2.2:+13667867 | None:intergenic |
| AATTCTCTCAGGCAAAGAAG+TGG | 0.596666 | 2.2:-13668271 | MS.gene69855:CDS |
| GGTACCCTCCAATTAAGCAA+TGG | 0.611167 | 2.2:+13667952 | None:intergenic |
| GCTTCAGCTTCTGTGATGTG+AGG | 0.642042 | 2.2:+13668295 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GAAAGATAATCTTAAGTTTT+GGG | - | chr2.2:13668526-13668545 | MS.gene69855:CDS | 20.0% |
| !!! | TGAAAGATAATCTTAAGTTT+TGG | - | chr2.2:13668525-13668544 | MS.gene69855:CDS | 20.0% |
| ! | AGTAATGTTGTTGAATGTAA+AGG | + | chr2.2:13667963-13667982 | None:intergenic | 25.0% |
| ! | TTGTTAATCCATTGCTTAAT+TGG | - | chr2.2:13668471-13668490 | MS.gene69855:CDS | 25.0% |
| !!! | TGAAGATGTTTTTTTGTTGT+TGG | + | chr2.2:13667940-13667959 | None:intergenic | 25.0% |
| AGATGTAACCGATACAAAAA+AGG | - | chr2.2:13668202-13668221 | MS.gene69855:CDS | 30.0% | |
| CTAAAAGGGTTTATGGATTT+GGG | - | chr2.2:13668305-13668324 | MS.gene69855:CDS | 30.0% | |
| TAATCCATTGCTTAATTGGA+GGG | - | chr2.2:13668475-13668494 | MS.gene69855:CDS | 30.0% | |
| TGAAACTGTTTGATATCAAC+TGG | - | chr2.2:13667895-13667914 | MS.gene69855:CDS | 30.0% | |
| TTAATCCATTGCTTAATTGG+AGG | - | chr2.2:13668474-13668493 | MS.gene69855:CDS | 30.0% | |
| TTGAGTTTAAGGAGCTAAAA+GGG | - | chr2.2:13668291-13668310 | MS.gene69855:CDS | 30.0% | |
| TTTATCAGAGCTTGAGTTTA+AGG | - | chr2.2:13668280-13668299 | MS.gene69855:CDS | 30.0% | |
| ! | TTGATGAAAGTGTGATTAGT+AGG | - | chr2.2:13668405-13668424 | MS.gene69855:CDS | 30.0% |
| AAAGACCTAACTTGAAGTGT+TGG | + | chr2.2:13668053-13668072 | None:intergenic | 35.0% | |
| CACTTTCATCAACATTGTGT+TGG | + | chr2.2:13668397-13668416 | None:intergenic | 35.0% | |
| CATAGAACGAGACTTAGAAA+AGG | - | chr2.2:13668239-13668258 | MS.gene69855:CDS | 35.0% | |
| CTTGAGTTTAAGGAGCTAAA+AGG | - | chr2.2:13668290-13668309 | MS.gene69855:CDS | 35.0% | |
| GAAAAGGAAGAAAAACTGCT+AGG | - | chr2.2:13668255-13668274 | MS.gene69855:CDS | 35.0% | |
| GCTAAAAGGGTTTATGGATT+TGG | - | chr2.2:13668304-13668323 | MS.gene69855:CDS | 35.0% | |
| GGAAGATAAAGATTCAGGAT+TGG | - | chr2.2:13668343-13668362 | MS.gene69855:CDS | 35.0% | |
| TAAGGAGCTAAAAGGGTTTA+TGG | - | chr2.2:13668298-13668317 | MS.gene69855:CDS | 35.0% | |
| TCTGAGGAAGATAAAGATTC+AGG | - | chr2.2:13668338-13668357 | MS.gene69855:CDS | 35.0% | |
| ! | GAATTCCAACACTTCAAGTT+AGG | - | chr2.2:13668045-13668064 | MS.gene69855:CDS | 35.0% |
| ! | TCTTTTAGCGACGAAAACTT+AGG | - | chr2.2:13668068-13668087 | MS.gene69855:CDS | 35.0% |
| !! | GATTGGTTTCTTTGATACCA+GGG | - | chr2.2:13668360-13668379 | MS.gene69855:CDS | 35.0% |
| !! | TAGTAGGCCTTATTTATCTG+AGG | - | chr2.2:13668421-13668440 | MS.gene69855:CDS | 35.0% |
| !! | TTTGGGATTTGTGTTTTCTG+AGG | - | chr2.2:13668322-13668341 | MS.gene69855:CDS | 35.0% |
| !!! | ATGACTCGCCTTTTTTGTAT+CGG | + | chr2.2:13668213-13668232 | None:intergenic | 35.0% |
| AATTCTCTCAGGCAAAGAAG+TGG | - | chr2.2:13668160-13668179 | MS.gene69855:CDS | 40.0% | |
| ACTTCTTTGCCTGAGAGAAT+TGG | + | chr2.2:13668161-13668180 | None:intergenic | 40.0% | |
| GAAAACTTAGGCACGAAGAT+TGG | - | chr2.2:13668080-13668099 | MS.gene69855:CDS | 40.0% | |
| TCAATCTCATTGCCAAGAGT+TGG | + | chr2.2:13668503-13668522 | None:intergenic | 40.0% | |
| TTCAACCTCTATGTCTCAAG+TGG | - | chr2.2:13667989-13668008 | MS.gene69855:CDS | 40.0% | |
| TTCTTCCACTTGAGACATAG+AGG | + | chr2.2:13667997-13668016 | None:intergenic | 40.0% | |
| ! | CCTTATTTATCTGAGGCTTG+GGG | - | chr2.2:13668428-13668447 | MS.gene69855:CDS | 40.0% |
| !! | GCCTTATTTATCTGAGGCTT+GGG | - | chr2.2:13668427-13668446 | MS.gene69855:CDS | 40.0% |
| !! | GGATTGGTTTCTTTGATACC+AGG | - | chr2.2:13668359-13668378 | MS.gene69855:CDS | 40.0% |
| AAGCTGAAGCCAATTCTCTC+AGG | - | chr2.2:13668149-13668168 | MS.gene69855:CDS | 45.0% | |
| CCCCAAGCCTCAGATAAATA+AGG | + | chr2.2:13668431-13668450 | None:intergenic | 45.0% | |
| GGTACCCTCCAATTAAGCAA+TGG | + | chr2.2:13668482-13668501 | None:intergenic | 45.0% | |
| TGTGATGTGAGGACAGAGTT+TGG | + | chr2.2:13668128-13668147 | None:intergenic | 45.0% | |
| !! | AATTGGAGGGTACCAACTCT+TGG | - | chr2.2:13668488-13668507 | MS.gene69855:CDS | 45.0% |
| !! | GGCCTTATTTATCTGAGGCT+TGG | - | chr2.2:13668426-13668445 | MS.gene69855:CDS | 45.0% |
| !!! | TGTTGGTTTTTGCTCAACCC+TGG | + | chr2.2:13668380-13668399 | None:intergenic | 45.0% |
| GCTTCAGCTTCTGTGATGTG+AGG | + | chr2.2:13668139-13668158 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.2 | gene | 13667876 | 13668577 | 13667876 | ID=MS.gene69855 |
| chr2.2 | mRNA | 13667876 | 13668577 | 13667876 | ID=MS.gene69855.t1;Parent=MS.gene69855 |
| chr2.2 | exon | 13667876 | 13668577 | 13667876 | ID=MS.gene69855.t1.exon1;Parent=MS.gene69855.t1 |
| chr2.2 | CDS | 13667876 | 13668577 | 13667876 | ID=cds.MS.gene69855.t1;Parent=MS.gene69855.t1 |
| Gene Sequence |
| Protein sequence |
