Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70273.t1 | PNY00086.1 | 71.4 | 105 | 19 | 2 | 1 | 102 | 1 | 97 | 4.80E-26 | 127.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70273.t1 | A0A2K3NAM0 | 71.4 | 105 | 19 | 2 | 1 | 102 | 1 | 97 | 3.4e-26 | 127.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054893 | MS.gene70273 | 0.804099 | 2.54E-49 | -1.69E-46 |
| MS.gene057093 | MS.gene70273 | 0.805147 | 1.54E-49 | -1.69E-46 |
| MS.gene057622 | MS.gene70273 | 0.812315 | 4.51E-51 | -1.69E-46 |
| MS.gene058592 | MS.gene70273 | 0.803588 | 3.24E-49 | -1.69E-46 |
| MS.gene059901 | MS.gene70273 | -0.800163 | 1.64E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70273.t1 | MTR_8g092580 | 86.607 | 112 | 7 | 2 | 1 | 104 | 14 | 125 | 5.51e-61 | 182 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAAAGATACAAGAGATCTT+TGG | 0.301924 | 4.1:+8978692 | MS.gene70273:CDS |
| CGATGCAGCACAAAAGCTTA+TGG | 0.339207 | 4.1:+8978556 | MS.gene70273:CDS |
| CTCACCCTTGTATCATTAAT+AGG | 0.341960 | 4.1:-8978794 | None:intergenic |
| CAAAGATACAAGAGATCTTT+GGG | 0.370184 | 4.1:+8978693 | MS.gene70273:CDS |
| TTCTCTTGTGAATATTTACA+TGG | 0.470533 | 4.1:+8978763 | MS.gene70273:CDS |
| CTACACCTATTAATGATACA+AGG | 0.485559 | 4.1:+8978789 | MS.gene70273:CDS |
| AAGGGCAACAACAACATTAG+AGG | 0.505052 | 4.1:+8978596 | MS.gene70273:CDS |
| ACAAAGTGATGATATTGTAA+TGG | 0.520018 | 4.1:+8978670 | MS.gene70273:CDS |
| CGAAGCAACCGACATGAAGA+AGG | 0.528099 | 4.1:+8978638 | MS.gene70273:CDS |
| GGATCTTAGTGATGAAGAGA+AGG | 0.549113 | 4.1:+8978577 | MS.gene70273:CDS |
| TGTATCATTAATAGGTGTAG+TGG | 0.560392 | 4.1:-8978786 | None:intergenic |
| TTGGCGTAAGATGAAGATTG+TGG | 0.597571 | 4.1:+8978499 | None:intergenic |
| GATCTTAGTGATGAAGAGAA+GGG | 0.605452 | 4.1:+8978578 | MS.gene70273:CDS |
| TACACCTATTAATGATACAA+GGG | 0.645512 | 4.1:+8978790 | MS.gene70273:CDS |
| TTAGAGGCAGAAGAAGCAAG+AGG | 0.689009 | 4.1:+8978612 | MS.gene70273:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACAAAGTGATGATATTGTAA+TGG | + | chr4.1:8978670-8978689 | MS.gene70273:CDS | 25.0% |
| ! | TACACCTATTAATGATACAA+GGG | + | chr4.1:8978790-8978809 | MS.gene70273:CDS | 25.0% |
| ! | TTCTCTTGTGAATATTTACA+TGG | + | chr4.1:8978763-8978782 | MS.gene70273:CDS | 25.0% |
| !!! | CTTTTTTGCTTCTTAACTAT+AGG | - | chr4.1:8978737-8978756 | None:intergenic | 25.0% |
| CAAAGATACAAGAGATCTTT+GGG | + | chr4.1:8978693-8978712 | MS.gene70273:CDS | 30.0% | |
| CTACACCTATTAATGATACA+AGG | + | chr4.1:8978789-8978808 | MS.gene70273:CDS | 30.0% | |
| TGTATCATTAATAGGTGTAG+TGG | - | chr4.1:8978789-8978808 | None:intergenic | 30.0% | |
| CTCACCCTTGTATCATTAAT+AGG | - | chr4.1:8978797-8978816 | None:intergenic | 35.0% | |
| GATCTTAGTGATGAAGAGAA+GGG | + | chr4.1:8978578-8978597 | MS.gene70273:CDS | 35.0% | |
| GCAAAGATACAAGAGATCTT+TGG | + | chr4.1:8978692-8978711 | MS.gene70273:CDS | 35.0% | |
| !!! | GCTTTTTTCCTTCTTCATGT+CGG | - | chr4.1:8978649-8978668 | None:intergenic | 35.0% |
| AAGGGCAACAACAACATTAG+AGG | + | chr4.1:8978596-8978615 | MS.gene70273:CDS | 40.0% | |
| GGATCTTAGTGATGAAGAGA+AGG | + | chr4.1:8978577-8978596 | MS.gene70273:CDS | 40.0% | |
| CGATGCAGCACAAAAGCTTA+TGG | + | chr4.1:8978556-8978575 | MS.gene70273:CDS | 45.0% | |
| TTAGAGGCAGAAGAAGCAAG+AGG | + | chr4.1:8978612-8978631 | MS.gene70273:CDS | 45.0% | |
| CGAAGCAACCGACATGAAGA+AGG | + | chr4.1:8978638-8978657 | MS.gene70273:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 8978509 | 8978823 | 8978509 | ID=MS.gene70273 |
| chr4.1 | mRNA | 8978509 | 8978823 | 8978509 | ID=MS.gene70273.t1;Parent=MS.gene70273 |
| chr4.1 | exon | 8978509 | 8978823 | 8978509 | ID=MS.gene70273.t1.exon1;Parent=MS.gene70273.t1 |
| chr4.1 | CDS | 8978509 | 8978823 | 8978509 | ID=cds.MS.gene70273.t1;Parent=MS.gene70273.t1 |
| Gene Sequence |
| Protein sequence |
