Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70343.t1 | XP_003630087.1 | 96 | 75 | 3 | 0 | 1 | 75 | 1 | 75 | 6.70E-30 | 139.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70343.t1 | G7LAG4 | 96.0 | 75 | 3 | 0 | 1 | 75 | 1 | 75 | 4.8e-30 | 139.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052166 | MS.gene70343 | 0.838899 | 2.19E-57 | -1.69E-46 |
| MS.gene060187 | MS.gene70343 | 0.805727 | 1.16E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70343.t1 | MTR_8g091590 | 96.000 | 75 | 3 | 0 | 1 | 75 | 1 | 75 | 1.51e-48 | 147 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 9 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCAATAGCAAATTGAGTTT+TGG | 0.254507 | 4.1:-9707479 | None:intergenic |
| GCAACTTGTTCAGAGTGTTT+TGG | 0.336230 | 4.1:+9707453 | MS.gene70343:CDS |
| GTCTTCTTGTTATTCACTAC+TGG | 0.369369 | 4.1:+9707405 | MS.gene70343:CDS |
| CTTCTTCTCCTCCTTCAACT+TGG | 0.390702 | 4.1:-9707431 | None:intergenic |
| ACTACTGGTTTCCAAGTTGA+AGG | 0.427104 | 4.1:+9707420 | MS.gene70343:CDS |
| CATTGCTATGTTAGTAGCTG+AGG | 0.547311 | 4.1:+9707309 | MS.gene70343:CDS |
| GTGGTGCTGCTGTAGAAACA+TGG | 0.583385 | 4.1:+9707371 | MS.gene70343:CDS |
| ACTGGTTTCCAAGTTGAAGG+AGG | 0.631072 | 4.1:+9707423 | MS.gene70343:CDS |
| TGGTGCTGCTGTAGAAACAT+GGG | 0.694208 | 4.1:+9707372 | MS.gene70343:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGAGTTTAAAAAATGCTGG+TGG | + | chr4.1:9707349-9707368 | MS.gene70343:CDS | 30.0% | |
| GTCAAGAGTTTAAAAAATGC+TGG | + | chr4.1:9707346-9707365 | MS.gene70343:CDS | 30.0% | |
| ! | AGCAATAGCAAATTGAGTTT+TGG | - | chr4.1:9707482-9707501 | None:intergenic | 30.0% |
| ! | GCAATAGCAAATTGAGTTTT+GGG | - | chr4.1:9707481-9707500 | None:intergenic | 30.0% |
| AGTTTAAAAAATGCTGGTGG+TGG | + | chr4.1:9707352-9707371 | MS.gene70343:CDS | 35.0% | |
| !! | GTCTTCTTGTTATTCACTAC+TGG | + | chr4.1:9707405-9707424 | MS.gene70343:CDS | 35.0% |
| CATTGCTATGTTAGTAGCTG+AGG | + | chr4.1:9707309-9707328 | MS.gene70343:CDS | 40.0% | |
| !! | ACTACTGGTTTCCAAGTTGA+AGG | + | chr4.1:9707420-9707439 | MS.gene70343:CDS | 40.0% |
| !! | GCAACTTGTTCAGAGTGTTT+TGG | + | chr4.1:9707453-9707472 | MS.gene70343:CDS | 40.0% |
| CTTCTTCTCCTCCTTCAACT+TGG | - | chr4.1:9707434-9707453 | None:intergenic | 45.0% | |
| ! | ACTGGTTTCCAAGTTGAAGG+AGG | + | chr4.1:9707423-9707442 | MS.gene70343:CDS | 45.0% |
| !! | TGGTGCTGCTGTAGAAACAT+GGG | + | chr4.1:9707372-9707391 | MS.gene70343:CDS | 45.0% |
| !! | GTGGTGCTGCTGTAGAAACA+TGG | + | chr4.1:9707371-9707390 | MS.gene70343:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 9707301 | 9707528 | 9707301 | ID=MS.gene70343 |
| chr4.1 | mRNA | 9707301 | 9707528 | 9707301 | ID=MS.gene70343.t1;Parent=MS.gene70343 |
| chr4.1 | exon | 9707301 | 9707528 | 9707301 | ID=MS.gene70343.t1.exon1;Parent=MS.gene70343.t1 |
| chr4.1 | CDS | 9707301 | 9707528 | 9707301 | ID=cds.MS.gene70343.t1;Parent=MS.gene70343.t1 |
| Gene Sequence |
| Protein sequence |
