Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71185.t1 | XP_003628802.1 | 67.5 | 77 | 13 | 2 | 1 | 77 | 1 | 65 | 7.50E-13 | 84 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71185.t1 | G7LHP4 | 67.5 | 77 | 13 | 2 | 1 | 77 | 1 | 65 | 5.4e-13 | 84.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053074 | MS.gene71185 | 0.838808 | 2.31E-57 | -1.69E-46 |
| MS.gene054184 | MS.gene71185 | 0.817005 | 4.13E-52 | -1.69E-46 |
| MS.gene054185 | MS.gene71185 | 0.804721 | 1.89E-49 | -1.69E-46 |
| MS.gene059294 | MS.gene71185 | 0.800822 | 1.20E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71185.t1 | MTR_8g066920 | 64.398 | 191 | 15 | 5 | 1 | 161 | 1 | 168 | 1.46e-42 | 139 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 67 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATCGTGATTCTGAGGATTT+TGG | 0.194043 | 8.3:-36004850 | MS.gene71185:CDS |
| TTGGGTGATGGAGGACTGCC+TGG | 0.249799 | 8.3:-36004721 | MS.gene71185:CDS |
| ACTGGATCCGGAGGACTTCT+TGG | 0.321037 | 8.3:-36004775 | MS.gene71185:CDS |
| CATTGCTCTCTTTGTGCTGT+TGG | 0.329983 | 8.3:-36004911 | MS.gene71185:CDS |
| GATCCAGTGGTTTGTGGAAC+TGG | 0.374467 | 8.3:+36004790 | None:intergenic |
| ATTGCTCTCTTTGTGCTGTT+GGG | 0.382762 | 8.3:-36004910 | MS.gene71185:CDS |
| GGAGTTCTGCCTGATGAAAA+AGG | 0.389577 | 8.3:-36004460 | MS.gene71185:CDS |
| TGTACCAGATGAAGGAGATT+TGG | 0.396430 | 8.3:-36004659 | MS.gene71185:CDS |
| CCCGTTGGTGCAGAAGTTCC+TGG | 0.413835 | 8.3:-36004562 | MS.gene71185:CDS |
| TGGGTGATGGAGGACTGCCT+GGG | 0.414674 | 8.3:-36004720 | MS.gene71185:CDS |
| TATCCTGGTGGTGTGAATTC+CGG | 0.423300 | 8.3:-36004601 | MS.gene71185:CDS |
| GGCAGAACTCCTCTTTCATC+AGG | 0.445935 | 8.3:+36004472 | None:intergenic |
| TCTTCCAGACCTCCTTCAAC+TGG | 0.449061 | 8.3:+36004505 | None:intergenic |
| CCTCCGGATCCAGTGGTTTG+TGG | 0.457277 | 8.3:+36004784 | None:intergenic |
| CCAGGAACTTCTGCACCAAC+GGG | 0.458513 | 8.3:+36004562 | None:intergenic |
| GATGGAGGACTGCCTGGTAC+TGG | 0.459909 | 8.3:-36004529 | MS.gene71185:CDS |
| ATAGGATCTGGAGCACTGCC+TGG | 0.462961 | 8.3:-36004694 | MS.gene71185:CDS |
| ACCAGGAACTTCTGCACCAA+CGG | 0.467348 | 8.3:+36004561 | None:intergenic |
| TGGACCAGTTGAAGGAGGTC+TGG | 0.467854 | 8.3:-36004509 | MS.gene71185:CDS |
| TGTACCAGGTGAAGAAGGTT+TGG | 0.467962 | 8.3:-36004740 | MS.gene71185:CDS |
| CCTGGTACTGGACCAGTTGA+AGG | 0.468919 | 8.3:-36004517 | MS.gene71185:CDS |
| GGACTTCTTGGAGGACTGCC+TGG | 0.469067 | 8.3:-36004763 | MS.gene71185:CDS |
| GGAGGACTGCCTGGTGTACC+AGG | 0.469604 | 8.3:-36004754 | MS.gene71185:CDS |
| GGAGGAGATGGAGGACTGCC+TGG | 0.473621 | 8.3:-36004535 | MS.gene71185:CDS |
| GTACCAGATGAAGGAGATTT+GGG | 0.475834 | 8.3:-36004658 | MS.gene71185:CDS |
| TCACCCAAATCTCCTTCATC+TGG | 0.480841 | 8.3:+36004655 | None:intergenic |
| GGTGAAGAAGGTTTGGGTGA+TGG | 0.490635 | 8.3:-36004733 | MS.gene71185:CDS |
| AAAGTACTTGGAGGACTGCC+TGG | 0.491036 | 8.3:-36004628 | MS.gene71185:CDS |
| GTTGAAGGAGGTCTGGAAGA+TGG | 0.497831 | 8.3:-36004502 | MS.gene71185:CDS |
| CCTTCAACTGGTCCAGTACC+AGG | 0.498932 | 8.3:+36004517 | None:intergenic |
| GGGTGATGGAGGACTGCCTG+GGG | 0.503356 | 8.3:-36004719 | MS.gene71185:CDS |
| GTTCCACAAACCACTGGATC+CGG | 0.504315 | 8.3:-36004787 | MS.gene71185:CDS |
| GTTCCTGGTGAAGGAGGAGA+TGG | 0.507827 | 8.3:-36004547 | MS.gene71185:CDS |
| GCACTGCCTGGAGGACTTCG+TGG | 0.511832 | 8.3:-36004682 | MS.gene71185:CDS |
| GAACTCCTCTTTCATCAGGC+AGG | 0.521690 | 8.3:+36004476 | None:intergenic |
| GAAGAAGGTTTGGGTGATGG+AGG | 0.528136 | 8.3:-36004730 | MS.gene71185:CDS |
| CAGTCCTCCAAGAAGTCCTC+CGG | 0.535457 | 8.3:+36004768 | None:intergenic |
| CCTTCTTCACCTGGTACACC+AGG | 0.538917 | 8.3:+36004745 | None:intergenic |
| CCTCCATCTCCTCCTTCACC+AGG | 0.541018 | 8.3:+36004544 | None:intergenic |
| CCTGGTGTACCAGGTGAAGA+AGG | 0.547230 | 8.3:-36004745 | MS.gene71185:CDS |
| GGACTGCCTGGTGAATATCC+TGG | 0.547709 | 8.3:-36004616 | MS.gene71185:CDS |
| GGTACACCACGAAGTCCTCC+AGG | 0.556740 | 8.3:+36004676 | None:intergenic |
| GTACCAGGTGAAGAAGGTTT+GGG | 0.563176 | 8.3:-36004739 | MS.gene71185:CDS |
| GGATCCGGAGGACTTCTTGG+AGG | 0.568359 | 8.3:-36004772 | MS.gene71185:CDS |
| GGAGTCCTGCCTGATGAAAG+AGG | 0.569327 | 8.3:-36004481 | MS.gene71185:CDS |
| CTTCCAGTTCCACAAACCAC+TGG | 0.572690 | 8.3:-36004793 | MS.gene71185:CDS |
| CTTCGTGGTGTACCAGATGA+AGG | 0.572822 | 8.3:-36004667 | MS.gene71185:CDS |
| CCACCGGAATTCACACCACC+AGG | 0.574503 | 8.3:+36004598 | None:intergenic |
| GGGTTGATAGCCGTCTCCAC+CGG | 0.580996 | 8.3:+36004582 | None:intergenic |
| CCTGGTGAAGGAGGAGATGG+AGG | 0.591303 | 8.3:-36004544 | MS.gene71185:CDS |
| GGTGTGAATTCCGGTGGAGA+CGG | 0.598585 | 8.3:-36004592 | MS.gene71185:CDS |
| GCAGAAGTTCCTGGTGAAGG+AGG | 0.602305 | 8.3:-36004553 | MS.gene71185:CDS |
| GGTGCAGAAGTTCCTGGTGA+AGG | 0.603113 | 8.3:-36004556 | MS.gene71185:CDS |
| CTACGACAATCGTGATTCTG+AGG | 0.610908 | 8.3:-36004857 | MS.gene71185:CDS |
| TCACCCAAACCTTCTTCACC+TGG | 0.611572 | 8.3:+36004736 | None:intergenic |
| AAGAAGTCCTCCGGATCCAG+TGG | 0.628933 | 8.3:+36004777 | None:intergenic |
| CAGTGGTTTGTGGAACTGGA+AGG | 0.642892 | 8.3:+36004794 | None:intergenic |
| ACACCACCAGGATATTCACC+AGG | 0.650451 | 8.3:+36004610 | None:intergenic |
| GGATCTGGAGCACTGCCTGG+AGG | 0.653536 | 8.3:-36004691 | MS.gene71185:CDS |
| CCTGGTGGTGTGAATTCCGG+TGG | 0.656979 | 8.3:-36004598 | MS.gene71185:CDS |
| GGTGATGGAGGACTGCCTGG+GGG | 0.657183 | 8.3:-36004718 | MS.gene71185:CDS |
| GGTACTGGACCAGTTGAAGG+AGG | 0.657439 | 8.3:-36004514 | MS.gene71185:CDS |
| GGAGACGGCTATCAACCCGT+TGG | 0.670699 | 8.3:-36004577 | MS.gene71185:CDS |
| CCACAAACCACTGGATCCGG+AGG | 0.679712 | 8.3:-36004784 | MS.gene71185:CDS |
| CTGCCTGGTGAATATCCTGG+TGG | 0.687649 | 8.3:-36004613 | MS.gene71185:CDS |
| TTTGACGGCTTTCAACCACA+AGG | 0.714605 | 8.3:-36004823 | MS.gene71185:CDS |
| ACTGGAAGGTGATAACCTTG+TGG | 0.757808 | 8.3:+36004808 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATACATAGCCTTTTTCATC+AGG | + | chr8.3:36004936-36004955 | None:intergenic | 30.0% |
| !! | AATCGTGATTCTGAGGATTT+TGG | - | chr8.3:36004534-36004553 | MS.gene71185:CDS | 35.0% |
| !! | TTGGGTGATGAAAAAGTACT+TGG | - | chr8.3:36004744-36004763 | MS.gene71185:CDS | 35.0% |
| GTACCAGATGAAGGAGATTT+GGG | - | chr8.3:36004726-36004745 | MS.gene71185:CDS | 40.0% | |
| TGTACCAGATGAAGGAGATT+TGG | - | chr8.3:36004725-36004744 | MS.gene71185:CDS | 40.0% | |
| ! | ATTGCTCTCTTTGTGCTGTT+GGG | - | chr8.3:36004474-36004493 | MS.gene71185:CDS | 40.0% |
| ! | GAGGATTTTGGACGATTTGA+CGG | - | chr8.3:36004546-36004565 | MS.gene71185:CDS | 40.0% |
| !! | GGTGATGAAAAAGTACTTGG+AGG | - | chr8.3:36004747-36004766 | MS.gene71185:CDS | 40.0% |
| ACTGGAAGGTGATAACCTTG+TGG | + | chr8.3:36004579-36004598 | None:intergenic | 45.0% | |
| CATTGCTCTCTTTGTGCTGT+TGG | - | chr8.3:36004473-36004492 | MS.gene71185:CDS | 45.0% | |
| CTACGACAATCGTGATTCTG+AGG | - | chr8.3:36004527-36004546 | MS.gene71185:CDS | 45.0% | |
| GGAGTTCTGCCTGATGAAAA+AGG | - | chr8.3:36004924-36004943 | MS.gene71185:CDS | 45.0% | |
| GTACCAGGTGAAGAAGGTTT+GGG | - | chr8.3:36004645-36004664 | MS.gene71185:CDS | 45.0% | |
| TCACCCAAATCTCCTTCATC+TGG | + | chr8.3:36004732-36004751 | None:intergenic | 45.0% | |
| TGTACCAGGTGAAGAAGGTT+TGG | - | chr8.3:36004644-36004663 | MS.gene71185:CDS | 45.0% | |
| TTTGACGGCTTTCAACCACA+AGG | - | chr8.3:36004561-36004580 | MS.gene71185:CDS | 45.0% | |
| ! | TATCCTGGTGGTGTGAATTC+CGG | - | chr8.3:36004783-36004802 | MS.gene71185:CDS | 45.0% |
| ACACCACCAGGATATTCACC+AGG | + | chr8.3:36004777-36004796 | None:intergenic | 50.0% | |
| ACCAGGAACTTCTGCACCAA+CGG | + | chr8.3:36004826-36004845 | None:intergenic | 50.0% | |
| CTTCCAGTTCCACAAACCAC+TGG | - | chr8.3:36004591-36004610 | MS.gene71185:CDS | 50.0% | |
| CTTCGTGGTGTACCAGATGA+AGG | - | chr8.3:36004717-36004736 | MS.gene71185:CDS | 50.0% | |
| GAACTCCTCTTTCATCAGGC+AGG | + | chr8.3:36004911-36004930 | None:intergenic | 50.0% | |
| GGCAGAACTCCTCTTTCATC+AGG | + | chr8.3:36004915-36004934 | None:intergenic | 50.0% | |
| GGTGAAGAAGGTTTGGGTGA+TGG | - | chr8.3:36004651-36004670 | MS.gene71185:CDS | 50.0% | |
| GTTCCACAAACCACTGGATC+CGG | - | chr8.3:36004597-36004616 | MS.gene71185:CDS | 50.0% | |
| GTTGAAGGAGGTCTGGAAGA+TGG | - | chr8.3:36004882-36004901 | MS.gene71185:CDS | 50.0% | |
| TCACCCAAACCTTCTTCACC+TGG | + | chr8.3:36004651-36004670 | None:intergenic | 50.0% | |
| TCTTCCAGACCTCCTTCAAC+TGG | + | chr8.3:36004882-36004901 | None:intergenic | 50.0% | |
| ! | AAAGTACTTGGAGGACTGCC+TGG | - | chr8.3:36004756-36004775 | MS.gene71185:CDS | 50.0% |
| ! | CAGTGGTTTGTGGAACTGGA+AGG | + | chr8.3:36004593-36004612 | None:intergenic | 50.0% |
| ! | GAAGAAGGTTTGGGTGATGG+AGG | - | chr8.3:36004654-36004673 | MS.gene71185:CDS | 50.0% |
| ! | GATCCAGTGGTTTGTGGAAC+TGG | + | chr8.3:36004597-36004616 | None:intergenic | 50.0% |
| AAGAAGTCCTCCGGATCCAG+TGG | + | chr8.3:36004610-36004629 | None:intergenic | 55.0% | |
| ACTGGATCCGGAGGACTTCT+TGG | - | chr8.3:36004609-36004628 | MS.gene71185:CDS | 55.0% | |
| CAGTCCTCCAAGAAGTCCTC+CGG | + | chr8.3:36004619-36004638 | None:intergenic | 55.0% | |
| CCAGGAACTTCTGCACCAAC+GGG | + | chr8.3:36004825-36004844 | None:intergenic | 55.0% | |
| CCTGGTGTACCAGGTGAAGA+AGG | - | chr8.3:36004639-36004658 | MS.gene71185:CDS | 55.0% | |
| CCTTCAACTGGTCCAGTACC+AGG | + | chr8.3:36004870-36004889 | None:intergenic | 55.0% | |
| CCTTCTTCACCTGGTACACC+AGG | + | chr8.3:36004642-36004661 | None:intergenic | 55.0% | |
| CTGCCTGGTGAATATCCTGG+TGG | - | chr8.3:36004771-36004790 | MS.gene71185:CDS | 55.0% | |
| GCAGAAGTTCCTGGTGAAGG+AGG | - | chr8.3:36004831-36004850 | MS.gene71185:CDS | 55.0% | |
| GGACTGCCTGGTGAATATCC+TGG | - | chr8.3:36004768-36004787 | MS.gene71185:CDS | 55.0% | |
| GGAGTCCTGCCTGATGAAAG+AGG | - | chr8.3:36004903-36004922 | MS.gene71185:CDS | 55.0% | |
| GGTGCAGAAGTTCCTGGTGA+AGG | - | chr8.3:36004828-36004847 | MS.gene71185:CDS | 55.0% | |
| GGTGTGAATTCCGGTGGAGA+CGG | - | chr8.3:36004792-36004811 | MS.gene71185:CDS | 55.0% | |
| GTTCCTGGTGAAGGAGGAGA+TGG | - | chr8.3:36004837-36004856 | MS.gene71185:CDS | 55.0% | |
| TGGACCAGTTGAAGGAGGTC+TGG | - | chr8.3:36004875-36004894 | MS.gene71185:CDS | 55.0% | |
| ! | CCTGGTACTGGACCAGTTGA+AGG | - | chr8.3:36004867-36004886 | MS.gene71185:CDS | 55.0% |
| ! | GGTACTGGACCAGTTGAAGG+AGG | - | chr8.3:36004870-36004889 | MS.gene71185:CDS | 55.0% |
| !! | ATAGGATCTGGAGCACTGCC+TGG | - | chr8.3:36004690-36004709 | MS.gene71185:CDS | 55.0% |
| CCACAAACCACTGGATCCGG+AGG | - | chr8.3:36004600-36004619 | MS.gene71185:CDS | 60.0% | |
| CCACCGGAATTCACACCACC+AGG | + | chr8.3:36004789-36004808 | None:intergenic | 60.0% | |
| CCCGTTGGTGCAGAAGTTCC+TGG | - | chr8.3:36004822-36004841 | MS.gene71185:CDS | 60.0% | |
| CCTCCATCTCCTCCTTCACC+AGG | + | chr8.3:36004843-36004862 | None:intergenic | 60.0% | |
| CCTCCGGATCCAGTGGTTTG+TGG | + | chr8.3:36004603-36004622 | None:intergenic | 60.0% | |
| CCTGGTGAAGGAGGAGATGG+AGG | - | chr8.3:36004840-36004859 | MS.gene71185:CDS | 60.0% | |
| GATGGAGGACTGCCTGGTAC+TGG | - | chr8.3:36004855-36004874 | MS.gene71185:CDS | 60.0% | |
| GCTCCAGATCCTATACCCCC+AGG | + | chr8.3:36004684-36004703 | None:intergenic | 60.0% | |
| GGACTTCTTGGAGGACTGCC+TGG | - | chr8.3:36004621-36004640 | MS.gene71185:CDS | 60.0% | |
| GGAGACGGCTATCAACCCGT+TGG | - | chr8.3:36004807-36004826 | MS.gene71185:CDS | 60.0% | |
| GGATCCGGAGGACTTCTTGG+AGG | - | chr8.3:36004612-36004631 | MS.gene71185:CDS | 60.0% | |
| GGTACACCACGAAGTCCTCC+AGG | + | chr8.3:36004711-36004730 | None:intergenic | 60.0% | |
| ! | CCTGGTGGTGTGAATTCCGG+TGG | - | chr8.3:36004786-36004805 | MS.gene71185:CDS | 60.0% |
| ! | CTGCCTGGGGGTATAGGATC+TGG | - | chr8.3:36004678-36004697 | MS.gene71185:CDS | 60.0% |
| !! | GGGTTGATAGCCGTCTCCAC+CGG | + | chr8.3:36004805-36004824 | None:intergenic | 60.0% |
| !! | TGGGTGATGGAGGACTGCCT+GGG | - | chr8.3:36004664-36004683 | MS.gene71185:CDS | 60.0% |
| !! | TTGGGTGATGGAGGACTGCC+TGG | - | chr8.3:36004663-36004682 | MS.gene71185:CDS | 60.0% |
| GCACTGCCTGGAGGACTTCG+TGG | - | chr8.3:36004702-36004721 | MS.gene71185:CDS | 65.0% | |
| GGAGGACTGCCTGGTGTACC+AGG | - | chr8.3:36004630-36004649 | MS.gene71185:CDS | 65.0% | |
| GGAGGAGATGGAGGACTGCC+TGG | - | chr8.3:36004849-36004868 | MS.gene71185:CDS | 65.0% | |
| ! | GGAGGACTGCCTGGGGGTAT+AGG | - | chr8.3:36004672-36004691 | MS.gene71185:CDS | 65.0% |
| !! | GGATCTGGAGCACTGCCTGG+AGG | - | chr8.3:36004693-36004712 | MS.gene71185:CDS | 65.0% |
| !! | GGGTGATGGAGGACTGCCTG+GGG | - | chr8.3:36004665-36004684 | MS.gene71185:CDS | 65.0% |
| !! | GGTGATGGAGGACTGCCTGG+GGG | - | chr8.3:36004666-36004685 | MS.gene71185:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 36004450 | 36004956 | 36004450 | ID=MS.gene71185 |
| chr8.3 | mRNA | 36004450 | 36004956 | 36004450 | ID=MS.gene71185.t1;Parent=MS.gene71185 |
| chr8.3 | exon | 36004450 | 36004956 | 36004450 | ID=MS.gene71185.t1.exon1;Parent=MS.gene71185.t1 |
| chr8.3 | CDS | 36004450 | 36004956 | 36004450 | ID=cds.MS.gene71185.t1;Parent=MS.gene71185.t1 |
| Gene Sequence |
| Protein sequence |
