Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71237.t1 | XP_024627457.1 | 98.4 | 245 | 4 | 0 | 282 | 526 | 68 | 312 | 8.90E-137 | 497.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71237.t1 | Q7VG78 | 39.6 | 187 | 110 | 1 | 328 | 511 | 784 | 970 | 6.2e-38 | 160.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71237.t1 | A0A396GM12 | 97.2 | 248 | 4 | 1 | 282 | 526 | 68 | 315 | 2.7e-135 | 491.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053144 | MS.gene71237 | 0.805903 | 1.07E-49 | -1.69E-46 |
| MS.gene054584 | MS.gene71237 | 0.824848 | 6.46E-54 | -1.69E-46 |
| MS.gene058206 | MS.gene71237 | 0.830338 | 3.11E-55 | -1.69E-46 |
| MS.gene060652 | MS.gene71237 | 0.816004 | 6.91E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71237.t1 | MTR_8g066040 | 90.370 | 270 | 9 | 2 | 274 | 526 | 60 | 329 | 2.51e-178 | 504 |
| MS.gene71237.t1 | MTR_0009s0230 | 69.200 | 250 | 75 | 1 | 277 | 526 | 61 | 308 | 2.56e-126 | 371 |
| MS.gene71237.t1 | MTR_0009s0230 | 68.800 | 250 | 75 | 2 | 277 | 526 | 61 | 307 | 1.66e-123 | 364 |
| MS.gene71237.t1 | MTR_4g130880 | 52.880 | 191 | 90 | 0 | 330 | 520 | 148 | 338 | 8.05e-73 | 236 |
| MS.gene71237.t1 | MTR_4g007070 | 51.337 | 187 | 91 | 0 | 330 | 516 | 137 | 323 | 4.03e-69 | 226 |
| MS.gene71237.t1 | MTR_2g063510 | 50.000 | 192 | 94 | 1 | 328 | 519 | 199 | 388 | 1.34e-67 | 223 |
| MS.gene71237.t1 | MTR_8g447000 | 43.777 | 233 | 124 | 3 | 292 | 519 | 152 | 382 | 4.02e-66 | 219 |
| MS.gene71237.t1 | MTR_3g111690 | 48.128 | 187 | 96 | 1 | 330 | 515 | 128 | 314 | 6.46e-62 | 206 |
| MS.gene71237.t1 | MTR_4g130880 | 43.220 | 118 | 65 | 1 | 330 | 447 | 148 | 263 | 3.10e-27 | 110 |
| MS.gene71237.t1 | MTR_2g063510 | 40.336 | 119 | 68 | 2 | 328 | 446 | 199 | 314 | 8.12e-24 | 102 |
| MS.gene71237.t1 | MTR_8g447000 | 36.129 | 155 | 90 | 4 | 292 | 439 | 152 | 304 | 8.76e-24 | 102 |
| MS.gene71237.t1 | MTR_4g065180 | 57.500 | 80 | 28 | 1 | 1 | 80 | 1 | 74 | 5.98e-20 | 85.1 |
| MS.gene71237.t1 | MTR_6g021620 | 34.884 | 86 | 43 | 1 | 1 | 73 | 1 | 86 | 5.45e-12 | 63.2 |
| MS.gene71237.t1 | MTR_7g451160 | 33.333 | 87 | 45 | 1 | 8 | 81 | 9 | 95 | 5.00e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71237.t1 | AT1G13635 | 69.478 | 249 | 74 | 1 | 278 | 526 | 65 | 311 | 1.29e-120 | 357 |
| MS.gene71237.t1 | AT1G13635 | 69.478 | 249 | 74 | 1 | 278 | 526 | 65 | 311 | 1.29e-120 | 357 |
| MS.gene71237.t1 | AT1G75090 | 51.020 | 196 | 96 | 0 | 326 | 521 | 115 | 310 | 9.01e-72 | 232 |
| MS.gene71237.t1 | AT5G57970 | 44.314 | 255 | 137 | 3 | 271 | 521 | 92 | 345 | 2.18e-71 | 231 |
| MS.gene71237.t1 | AT5G57970 | 44.314 | 255 | 137 | 3 | 271 | 521 | 92 | 345 | 2.18e-71 | 231 |
| MS.gene71237.t1 | AT5G44680 | 44.635 | 233 | 122 | 2 | 292 | 519 | 121 | 351 | 1.80e-69 | 227 |
| MS.gene71237.t1 | AT1G15970 | 48.168 | 191 | 99 | 0 | 330 | 520 | 145 | 335 | 2.35e-65 | 216 |
| MS.gene71237.t1 | AT1G15970 | 48.168 | 191 | 99 | 0 | 330 | 520 | 145 | 335 | 2.35e-65 | 216 |
| MS.gene71237.t1 | AT3G12710 | 43.231 | 229 | 127 | 2 | 295 | 522 | 86 | 312 | 8.31e-63 | 208 |
| MS.gene71237.t1 | AT1G80850 | 45.550 | 191 | 104 | 0 | 330 | 520 | 134 | 324 | 1.31e-62 | 208 |
Find 111 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACTAGATACAGAAATTT+TGG | 0.173060 | 8.3:-36823081 | MS.gene71237:CDS |
| TTGCTTAGAGCCTCTGCTTT+TGG | 0.204125 | 8.3:+36814036 | None:intergenic |
| GATTTCCTGTTCCTCCATTT+TGG | 0.208004 | 8.3:+36814764 | None:intergenic |
| TCCTTACACTGTTGCCAAAA+TGG | 0.236844 | 8.3:-36814778 | MS.gene71237:CDS |
| TATTTGTATTTGTTAATTAT+TGG | 0.253663 | 8.3:+36814084 | None:intergenic |
| CTCCTTAATGGGACACTTCT+TGG | 0.263263 | 8.3:+36814060 | None:intergenic |
| CGTAAACGTCTTCTTCTCTT+TGG | 0.273518 | 8.3:+36822993 | None:intergenic |
| ATCTTCATCAACAGGTTGTT+AGG | 0.301024 | 8.3:-36814155 | MS.gene71237:intron |
| ACCCAAGAAGTGTCCCATTA+AGG | 0.321221 | 8.3:-36814062 | MS.gene71237:CDS |
| AGCTGATTCTTTGACTCCAT+TGG | 0.322748 | 8.3:-36818650 | MS.gene71237:CDS |
| TGAGGAAACAAAGGAAGATT+TGG | 0.328451 | 8.3:-36823177 | MS.gene71237:CDS |
| GATCGAAGATTCGGCGGTTT+CGG | 0.331126 | 8.3:+36822127 | None:intergenic |
| TATGGCGTTTAAAGTCTAAA+TGG | 0.331232 | 8.3:-36823223 | MS.gene71237:CDS |
| ATTTACCTTCATTATGCATT+TGG | 0.335454 | 8.3:+36814674 | None:intergenic |
| TTGTACTGAACTTTATTTCA+AGG | 0.359766 | 8.3:-36823339 | MS.gene71237:CDS |
| AGATTCGGCGGTTTCGGTGG+TGG | 0.373491 | 8.3:+36822133 | None:intergenic |
| ACATGGTAAAACGTGGATTT+CGG | 0.376776 | 8.3:-36814011 | MS.gene71237:CDS |
| TTCATTAGTGATCATTCAAT+TGG | 0.378426 | 8.3:-36821594 | MS.gene71237:CDS |
| GATTCGGCGGTTTCGGTGGT+GGG | 0.381401 | 8.3:+36822134 | None:intergenic |
| TTTGAGATGCTTGCAATGTC+TGG | 0.382865 | 8.3:-36814972 | MS.gene71237:CDS |
| TCCTTAATGGGACACTTCTT+GGG | 0.390688 | 8.3:+36814061 | None:intergenic |
| CTTTGTTATTAATATATTCA+AGG | 0.392471 | 8.3:+36821556 | None:intergenic |
| GGGAATTGAAAAGGTGCAAC+TGG | 0.400524 | 8.3:-36818513 | MS.gene71237:CDS |
| CTTTGACTCCATTGGATGAA+AGG | 0.411007 | 8.3:-36818642 | MS.gene71237:CDS |
| TGGCTTCTCAACAGACTTCA+AGG | 0.414990 | 8.3:+36823151 | None:intergenic |
| GACTTAACAAAGTGTGAATC+TGG | 0.415650 | 8.3:+36822235 | None:intergenic |
| GGCGGTTTCGGTGGTGGGGA+TGG | 0.423361 | 8.3:+36822139 | None:intergenic |
| TAGAATTTCATGATGAATGC+TGG | 0.424817 | 8.3:-36815491 | MS.gene71237:CDS |
| TGTTGCCAAAATGGAGGAAC+AGG | 0.430065 | 8.3:-36814769 | MS.gene71237:CDS |
| TGATGATGGTTGATGTTTGA+TGG | 0.433310 | 8.3:+36822183 | None:intergenic |
| GATTGCTCATGGACTACAAT+TGG | 0.434181 | 8.3:-36814950 | MS.gene71237:CDS |
| AATCTGGTGGTTTCGAGGGT+GGG | 0.435047 | 8.3:+36822251 | None:intergenic |
| GGCGGTGTCGATCGAAGATT+CGG | 0.435415 | 8.3:+36822118 | None:intergenic |
| GATGGTGGTGGTTGAGTTAT+CGG | 0.448475 | 8.3:+36822307 | None:intergenic |
| ATCTGGTGGTTTCGAGGGTG+GGG | 0.449105 | 8.3:+36822252 | None:intergenic |
| GGTGGGGACCGAGGTACCGG+AGG | 0.452544 | 8.3:+36822268 | None:intergenic |
| GAGGGTGGGGACCGAGGTAC+CGG | 0.456079 | 8.3:+36822265 | None:intergenic |
| TGCAATGTCTGGATTGCTCA+TGG | 0.456628 | 8.3:-36814961 | MS.gene71237:CDS |
| CATACAAATTATTACCTAAG+TGG | 0.470871 | 8.3:+36814903 | None:intergenic |
| TCTTCATCAACAGGTTGTTA+GGG | 0.472618 | 8.3:-36814154 | MS.gene71237:intron |
| CCTATAGCAACACGTCGATC+AGG | 0.472784 | 8.3:+36822079 | None:intergenic |
| GATCATTTAGTAGCTACATA+TGG | 0.478959 | 8.3:-36814125 | MS.gene71237:CDS |
| ACATAATCAGAACTTATAGA+TGG | 0.482039 | 8.3:+36823131 | None:intergenic |
| AAAACGTGGATTTCGGTTTG+TGG | 0.483743 | 8.3:-36814004 | MS.gene71237:CDS |
| CAGTAGTAAAAGTGTTCCAT+TGG | 0.493076 | 8.3:+36823252 | None:intergenic |
| AAACGTGGATTTCGGTTTGT+GGG | 0.497995 | 8.3:-36814003 | MS.gene71237:CDS |
| TGATGGTATTCTCCATAACC+TGG | 0.500246 | 8.3:+36822200 | None:intergenic |
| ATTCGGCGGTTTCGGTGGTG+GGG | 0.503171 | 8.3:+36822135 | None:intergenic |
| GAATCTGGTGGTTTCGAGGG+TGG | 0.503656 | 8.3:+36822250 | None:intergenic |
| TAATGAAGCATGTGGTCTGC+TGG | 0.506129 | 8.3:+36821610 | None:intergenic |
| TGCAAGGCCGTTCTCTCCAA+TGG | 0.507260 | 8.3:-36823268 | MS.gene71237:CDS |
| AGAATTTCATGATGAATGCT+GGG | 0.513589 | 8.3:-36815490 | MS.gene71237:CDS |
| CAACAGGTTGTTAGGGAATG+TGG | 0.514316 | 8.3:-36814147 | MS.gene71237:intron |
| ACTTACTTGTCGTCATAAGC+TGG | 0.516840 | 8.3:+36815462 | None:intergenic |
| ATAAAAGAGTGCACTATCAC+TGG | 0.517350 | 8.3:+36813979 | None:intergenic |
| GCCTCCAAGGTCTTTCTGCT+AGG | 0.519579 | 8.3:+36813893 | None:intergenic |
| ATTGACCATTGATCATCTTG+TGG | 0.520664 | 8.3:-36813944 | MS.gene71237:CDS |
| TTTCAGCTTCTTCACATGAA+AGG | 0.522846 | 8.3:-36818597 | MS.gene71237:CDS |
| CGAAGATTCGGCGGTTTCGG+TGG | 0.524716 | 8.3:+36822130 | None:intergenic |
| CTGAGCCAGGGGAATTGAAA+AGG | 0.526681 | 8.3:-36818522 | MS.gene71237:CDS |
| CCACTAGTGAGTACTGAGCC+AGG | 0.527168 | 8.3:-36818535 | MS.gene71237:CDS |
| GGAAACAAGAATATCTGAGT+TGG | 0.527863 | 8.3:-36823318 | MS.gene71237:CDS |
| ACAATCCACAAGATGATCAA+TGG | 0.535979 | 8.3:+36813939 | None:intergenic |
| CAATGCCAAATGCATAATGA+AGG | 0.541462 | 8.3:-36814679 | MS.gene71237:intron |
| ATGATTCCCCTCCGGTACCT+CGG | 0.543683 | 8.3:-36822276 | MS.gene71237:CDS |
| ACGGAAACGAAGATGCCTAC+TGG | 0.543939 | 8.3:-36823370 | MS.gene71237:CDS |
| GGTGGTTTCTTCTGAGGCGG+CGG | 0.547617 | 8.3:+36822100 | None:intergenic |
| TAAGCCTAGCAGAAAGACCT+TGG | 0.551983 | 8.3:-36813897 | MS.gene71237:CDS |
| GGTTTCGAGGGTGGGGACCG+AGG | 0.554048 | 8.3:+36822259 | None:intergenic |
| GATGAAATCCTTTCATCCAA+TGG | 0.555797 | 8.3:+36818634 | None:intergenic |
| CGATCAGGTGGTTTCTTCTG+AGG | 0.558384 | 8.3:+36822094 | None:intergenic |
| TTGGAGAGAACGGCCTTGCA+TGG | 0.559053 | 8.3:+36823271 | None:intergenic |
| CCTGGCTCAGTACTCACTAG+TGG | 0.563648 | 8.3:+36818535 | None:intergenic |
| AGAACGGCCTTGCATGGTCA+AGG | 0.565120 | 8.3:+36823277 | None:intergenic |
| GTGTGAATCTGGTGGTTTCG+AGG | 0.566644 | 8.3:+36822246 | None:intergenic |
| TTCACACTTTGTTAAGTCGA+CGG | 0.571287 | 8.3:-36822230 | MS.gene71237:CDS |
| TGAAATAAAGTTCAGTACAA+AGG | 0.575186 | 8.3:+36823342 | None:intergenic |
| AGATGTGTTGCTGATTAAAG+TGG | 0.576003 | 8.3:-36821535 | MS.gene71237:CDS |
| TGGGGACCGAGGTACCGGAG+GGG | 0.577816 | 8.3:+36822270 | None:intergenic |
| AAGTGTTCCATTGGAGAGAA+CGG | 0.577941 | 8.3:+36823261 | None:intergenic |
| TTTGTTTCCTCATCTAACAC+CGG | 0.580159 | 8.3:+36823188 | None:intergenic |
| GTGGGGACCGAGGTACCGGA+GGG | 0.582204 | 8.3:+36822269 | None:intergenic |
| GTCAATCTATGATTCCCCTC+CGG | 0.591970 | 8.3:-36822284 | MS.gene71237:CDS |
| CACTAGTGAGTACTGAGCCA+GGG | 0.593056 | 8.3:-36818534 | MS.gene71237:CDS |
| GCCTAGCAGAAAGACCTTGG+AGG | 0.593814 | 8.3:-36813894 | MS.gene71237:CDS |
| TCATAGATTGACGATGGTGG+TGG | 0.600339 | 8.3:+36822295 | None:intergenic |
| ATGGTTGCCTTGACCATGCA+AGG | 0.603294 | 8.3:-36823284 | MS.gene71237:CDS |
| TGTGAATCTGGTGGTTTCGA+GGG | 0.606013 | 8.3:+36822247 | None:intergenic |
| AAAAGCAGAGGCTCTAAGCA+AGG | 0.611659 | 8.3:-36814034 | MS.gene71237:CDS |
| GTGAAAACATAGATTGATGA+TGG | 0.616039 | 8.3:+36822169 | None:intergenic |
| TGGTGTTTCTTGAAGAACGA+TGG | 0.617014 | 8.3:+36823013 | None:intergenic |
| TCAGGTGGTTTCTTCTGAGG+CGG | 0.627515 | 8.3:+36822097 | None:intergenic |
| GTTAGTTAGATATGCCTCCA+AGG | 0.629114 | 8.3:+36813880 | None:intergenic |
| ATAGATAGTATTCCCCATGC+CGG | 0.636155 | 8.3:-36821495 | MS.gene71237:intron |
| TGAGTTGGCAAAGATGATGA+TGG | 0.643209 | 8.3:-36823303 | MS.gene71237:CDS |
| TTAACAAAGTGTGAATCTGG+TGG | 0.643416 | 8.3:+36822238 | None:intergenic |
| AGAGGCTCTAAGCAAGGACA+TGG | 0.646875 | 8.3:-36814028 | MS.gene71237:CDS |
| TTACACTGTTGCCAAAATGG+AGG | 0.656370 | 8.3:-36814775 | MS.gene71237:CDS |
| ACTAGTGAGTACTGAGCCAG+GGG | 0.660813 | 8.3:-36818533 | MS.gene71237:CDS |
| GAAACGAAGATGCCTACTGG+TGG | 0.663138 | 8.3:-36823367 | MS.gene71237:CDS |
| ATTAAGGAGTCCAAAAGCAG+AGG | 0.668988 | 8.3:-36814046 | MS.gene71237:CDS |
| GGTGTTAGATGAGGAAACAA+AGG | 0.671858 | 8.3:-36823186 | MS.gene71237:CDS |
| ATAGCAACACGTCGATCAGG+TGG | 0.672470 | 8.3:+36822082 | None:intergenic |
| GGTGTCGATCGAAGATTCGG+CGG | 0.674041 | 8.3:+36822121 | None:intergenic |
| AGCAAGGACATGGTAAAACG+TGG | 0.674831 | 8.3:-36814018 | MS.gene71237:CDS |
| AGTACAAAGGATCCACCAGT+AGG | 0.676451 | 8.3:+36823355 | None:intergenic |
| TGTGTTGCTGATTAAAGTGG+AGG | 0.677590 | 8.3:-36821532 | MS.gene71237:CDS |
| GGGGAATCATAGATTGACGA+TGG | 0.683492 | 8.3:+36822289 | None:intergenic |
| GAATCATAGATTGACGATGG+TGG | 0.683775 | 8.3:+36822292 | None:intergenic |
| GAATTTCATGATGAATGCTG+GGG | 0.685294 | 8.3:-36815489 | MS.gene71237:CDS |
| ATGATCACTAATGAAGCATG+TGG | 0.731158 | 8.3:+36821602 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 36813885 | 36823411 | 36813885 | ID=MS.gene71237 |
| chr8.3 | mRNA | 36813885 | 36823411 | 36813885 | ID=MS.gene71237.t1;Parent=MS.gene71237 |
| chr8.3 | exon | 36823000 | 36823411 | 36823000 | ID=MS.gene71237.t1.exon1;Parent=MS.gene71237.t1 |
| chr8.3 | CDS | 36823000 | 36823411 | 36823000 | ID=cds.MS.gene71237.t1;Parent=MS.gene71237.t1 |
| chr8.3 | exon | 36822092 | 36822340 | 36822092 | ID=MS.gene71237.t1.exon2;Parent=MS.gene71237.t1 |
| chr8.3 | CDS | 36822092 | 36822340 | 36822092 | ID=cds.MS.gene71237.t1;Parent=MS.gene71237.t1 |
| chr8.3 | exon | 36821496 | 36821636 | 36821496 | ID=MS.gene71237.t1.exon3;Parent=MS.gene71237.t1 |
| chr8.3 | CDS | 36821496 | 36821636 | 36821496 | ID=cds.MS.gene71237.t1;Parent=MS.gene71237.t1 |
| chr8.3 | exon | 36818497 | 36818712 | 36818497 | ID=MS.gene71237.t1.exon4;Parent=MS.gene71237.t1 |
| chr8.3 | CDS | 36818497 | 36818712 | 36818497 | ID=cds.MS.gene71237.t1;Parent=MS.gene71237.t1 |
| chr8.3 | exon | 36815468 | 36815525 | 36815468 | ID=MS.gene71237.t1.exon5;Parent=MS.gene71237.t1 |
| chr8.3 | CDS | 36815468 | 36815525 | 36815468 | ID=cds.MS.gene71237.t1;Parent=MS.gene71237.t1 |
| chr8.3 | exon | 36814918 | 36815001 | 36814918 | ID=MS.gene71237.t1.exon6;Parent=MS.gene71237.t1 |
| chr8.3 | CDS | 36814918 | 36815001 | 36814918 | ID=cds.MS.gene71237.t1;Parent=MS.gene71237.t1 |
| chr8.3 | exon | 36814680 | 36814821 | 36814680 | ID=MS.gene71237.t1.exon7;Parent=MS.gene71237.t1 |
| chr8.3 | CDS | 36814680 | 36814821 | 36814680 | ID=cds.MS.gene71237.t1;Parent=MS.gene71237.t1 |
| chr8.3 | exon | 36813885 | 36814163 | 36813885 | ID=MS.gene71237.t1.exon8;Parent=MS.gene71237.t1 |
| chr8.3 | CDS | 36813885 | 36814163 | 36813885 | ID=cds.MS.gene71237.t1;Parent=MS.gene71237.t1 |
| Gene Sequence |
| Protein sequence |
