AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene060652

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060652.t1 MTR_2g078210 94.855 311 10 2 1 305 6 316 0.0 604
MS.gene060652.t1 MTR_2g078210 96.484 256 9 0 50 305 1 256 0.0 516
MS.gene060652.t1 MTR_2g015370 47.279 294 134 6 1 285 1 282 8.39e-81 247
MS.gene060652.t1 MTR_4g050480 43.636 165 93 0 65 229 98 262 3.09e-34 129
MS.gene060652.t1 MTR_4g050480 43.636 165 93 0 65 229 99 263 3.16e-34 129
MS.gene060652.t1 MTR_1g095740 37.427 171 97 4 64 227 142 309 8.24e-30 118
MS.gene060652.t1 MTR_6g033415 36.517 178 102 4 57 227 101 274 1.02e-28 114
MS.gene060652.t1 MTR_1g066800 35.260 173 102 3 56 223 109 276 9.82e-27 109
MS.gene060652.t1 MTR_1g066800 35.758 165 96 3 64 223 122 281 1.06e-26 109
MS.gene060652.t1 MTR_7g100790 35.754 179 110 2 64 241 109 283 3.19e-25 105
MS.gene060652.t1 MTR_3g104570 36.872 179 103 5 65 234 93 270 9.58e-24 100
MS.gene060652.t1 MTR_4g087420 31.892 185 106 5 54 223 137 316 3.18e-20 91.3
MS.gene060652.t1 MTR_8g024540 31.183 186 102 8 63 229 127 305 8.37e-16 78.2
MS.gene060652.t1 MTR_8g017570 30.570 193 126 3 65 249 92 284 1.27e-14 74.3
MS.gene060652.t1 MTR_2g099780 32.370 173 107 3 65 227 80 252 6.54e-12 66.2
MS.gene060652.t1 MTR_1g007920 27.692 195 114 5 65 232 99 293 3.03e-11 64.3
MS.gene060652.t1 MTR_1g007920 28.205 195 113 5 65 232 124 318 4.17e-11 63.9
MS.gene060652.t1 MTR_0047s0040 28.313 166 108 4 59 221 97 254 1.00e-10 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060652.t1 AT3G15050 54.741 232 92 3 1 232 1 219 6.70e-82 248
MS.gene060652.t1 AT2G33990 49.500 200 96 3 65 261 49 246 3.98e-64 203
MS.gene060652.t1 AT3G22190 36.161 224 140 2 63 284 88 310 5.68e-33 126
MS.gene060652.t1 AT3G22190 36.161 224 140 2 63 284 88 310 5.68e-33 126
MS.gene060652.t1 AT3G22190 36.161 224 140 2 63 284 88 310 5.68e-33 126
MS.gene060652.t1 AT3G22190 36.161 224 140 2 63 284 88 310 5.68e-33 126
MS.gene060652.t1 AT2G26180 40.805 174 95 3 65 230 86 259 3.57e-31 121
MS.gene060652.t1 AT2G26410 35.882 170 100 4 65 228 150 316 5.52e-28 113
MS.gene060652.t1 AT2G26410 35.882 170 100 4 65 228 150 316 5.52e-28 113
MS.gene060652.t1 AT3G52290 35.057 174 108 3 56 227 101 271 1.58e-27 111
MS.gene060652.t1 AT5G03040 31.140 228 137 6 9 227 64 280 8.03e-27 109
MS.gene060652.t1 AT5G03040 31.140 228 137 6 9 227 64 280 8.03e-27 109
MS.gene060652.t1 AT5G03040 31.140 228 137 6 9 227 64 280 8.03e-27 109
MS.gene060652.t1 AT1G72670 30.952 210 133 4 31 230 57 264 6.94e-26 106
MS.gene060652.t1 AT3G09710 35.119 168 100 2 65 227 110 273 1.18e-25 106
MS.gene060652.t1 AT3G09710 33.516 182 98 4 65 227 110 287 4.70e-25 104
MS.gene060652.t1 AT3G59690 33.140 172 102 7 51 219 157 318 2.07e-23 100
MS.gene060652.t1 AT3G59690 33.140 172 102 7 51 219 157 318 2.07e-23 100
MS.gene060652.t1 AT1G17480 31.429 175 98 4 46 218 85 239 9.05e-22 94.4
MS.gene060652.t1 AT2G43680 32.044 181 91 8 51 219 310 470 2.25e-17 82.8
MS.gene060652.t1 AT2G43680 32.044 181 91 8 51 219 310 470 2.25e-17 82.8
MS.gene060652.t1 AT2G43680 32.044 181 91 8 51 219 310 470 2.25e-17 82.8
MS.gene060652.t1 AT2G43680 32.044 181 91 8 51 219 310 470 2.25e-17 82.8
MS.gene060652.t1 AT2G43680 32.044 181 91 8 51 219 311 471 2.35e-17 82.8
MS.gene060652.t1 AT3G49260 25.888 197 120 4 65 241 122 312 3.57e-17 82.0
MS.gene060652.t1 AT3G49260 25.888 197 120 4 65 241 122 312 3.57e-17 82.0
MS.gene060652.t1 AT3G49260 25.888 197 120 4 65 241 122 312 3.57e-17 82.0
MS.gene060652.t1 AT3G49260 26.263 198 119 4 65 241 122 313 5.40e-17 81.6
MS.gene060652.t1 AT4G00820 27.014 211 125 6 38 230 112 311 1.00e-15 77.8
MS.gene060652.t1 AT1G01110 24.775 222 144 7 28 230 86 303 1.17e-12 68.6
MS.gene060652.t1 AT4G10640 29.016 193 98 6 65 227 102 285 7.28e-11 62.8

Find 75 sgRNAs with CRISPR-Local

Find 184 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATGCATTTCGTTCATTTA+TGG 0.156996 2.2:+16488903 MS.gene060652:CDS
GCCGTTGAGTTTCTTTGTTT+TGG 0.177224 2.2:-16490734 None:intergenic
GTGGTTCATCTTATCTAATT+TGG 0.248605 2.2:-16490770 None:intergenic
GAAGAACATTTCAACATCTA+AGG 0.257374 2.2:+16490086 MS.gene060652:CDS
CCAAACTGTAACCAGTATTT+TGG 0.272065 2.2:+16490610 MS.gene060652:CDS
CAGCGCGCCCTTGGGAGGTT+CGG 0.278767 2.2:+16490695 MS.gene060652:CDS
CTCCATCATCAAACCTTCAT+TGG 0.319760 2.2:-16488830 None:intergenic
CTAATTTGGTCTTCTGCTGC+TGG 0.344502 2.2:-16490756 None:intergenic
TGTTGTTCCTGTTTGGTCTC+TGG 0.347194 2.2:-16490149 None:intergenic
GTTGGGTTGCAGCGCGCCCT+TGG 0.356093 2.2:+16490686 MS.gene060652:CDS
TGGAGAGGACAGTTCCTTCC+AGG 0.372133 2.2:+16488848 MS.gene060652:CDS
CCAAAATACTGGTTACAGTT+TGG 0.386021 2.2:-16490610 None:intergenic
CACTTAACTACATACATTCA+TGG 0.393069 2.2:+16490170 MS.gene060652:CDS
GGCATATGCTTTCTCTCATC+AGG 0.394693 2.2:+16490506 MS.gene060652:CDS
ACTAAAGCTGCCATGTCAAA+TGG 0.395308 2.2:+16490826 MS.gene060652:CDS
TTGGGTTGCAGCGCGCCCTT+GGG 0.409869 2.2:+16490687 MS.gene060652:CDS
ATGAAGCCTGACCAAAATAC+TGG 0.422336 2.2:-16490621 None:intergenic
AGTTAAGTGTTGTTCCTGTT+TGG 0.425963 2.2:-16490156 None:intergenic
TTGGAGCTGGATGGAACGTT+GGG 0.427818 2.2:+16490669 MS.gene060652:CDS
CAAGAATGGGTCCAAAGAAA+TGG 0.430866 2.2:+16488182 None:intergenic
TTGATTCTTCATGAGGGCTA+TGG 0.431764 2.2:-16488798 None:intergenic
AGCGCGCCCTTGGGAGGTTC+GGG 0.435357 2.2:+16490696 MS.gene060652:CDS
AGGGCTATGGTTCCCATTGG+AGG 0.450209 2.2:-16488785 None:intergenic
TCCATCATCAAACCTTCATT+GGG 0.455643 2.2:-16488829 None:intergenic
GACATGGCAGCTTTAGTCAA+TGG 0.463459 2.2:-16490820 None:intergenic
AAGAAAGCTGGGGTTGGAGC+TGG 0.466713 2.2:+16490656 MS.gene060652:CDS
CAGGATTAAGCAAAAGAGAT+TGG 0.472339 2.2:+16490246 MS.gene060652:CDS
CTAAAGCTGCCATGTCAAAT+GGG 0.486923 2.2:+16490827 MS.gene060652:CDS
ATGAGGGCTATGGTTCCCAT+TGG 0.494385 2.2:-16488788 None:intergenic
AAGAACATTTCAACATCTAA+GGG 0.495562 2.2:+16490087 MS.gene060652:CDS
GTGTTACTTGATTCTTCATG+AGG 0.498076 2.2:-16488805 None:intergenic
GTTGGAGCTGGATGGAACGT+TGG 0.504247 2.2:+16490668 MS.gene060652:CDS
ATAGCCTTGGTAAAGAAAGC+TGG 0.504499 2.2:+16490644 MS.gene060652:CDS
GGTCAGGCTTCATATAGCCT+TGG 0.512035 2.2:+16490631 MS.gene060652:CDS
TTCACAATATTGCTGCTACT+CGG 0.514388 2.2:+16488875 MS.gene060652:CDS
TTGGTAAAGAAAGCTGGGGT+TGG 0.518496 2.2:+16490650 MS.gene060652:CDS
AGATAAATCAAACCAAGAAA+AGG 0.519572 2.2:+16488240 MS.gene060652:CDS
GGTTGCAGCGCGCCCTTGGG+AGG 0.525212 2.2:+16490690 MS.gene060652:CDS
TTATAATCAGAAGGAAGAAA+CGG 0.527194 2.2:+16488212 MS.gene060652:CDS
TAGCCTTGGTAAAGAAAGCT+GGG 0.527594 2.2:+16490645 MS.gene060652:CDS
GGGCTATGGTTCCCATTGGA+GGG 0.533590 2.2:-16488784 None:intergenic
TCAAGTAACACTCCCAATGA+AGG 0.535092 2.2:+16488817 MS.gene060652:CDS
ACTTGAACCCGAACCTCCCA+AGG 0.538007 2.2:-16490703 None:intergenic
TCCCAATGAAGGTTTGATGA+TGG 0.538229 2.2:+16488828 MS.gene060652:CDS
CAATCTACTGAACCCTCCAA+TGG 0.545908 2.2:+16488772 MS.gene060652:CDS
GTGCAGCGGTTCTGAAACAA+TGG 0.546419 2.2:+16490422 MS.gene060652:CDS
ATCGCCATTAAAATCACCAT+TGG 0.547703 2.2:+16490867 MS.gene060652:CDS
AATCTACTGAACCCTCCAAT+GGG 0.550183 2.2:+16488773 MS.gene060652:CDS
GACACGGAAGCTTTAGCCAA+TGG 0.552731 2.2:-16490883 None:intergenic
CACATGGCCAGAGACCAAAC+AGG 0.554081 2.2:+16490142 MS.gene060652:CDS
AATGCGAAGGAGACTGAGAA+AGG 0.557321 2.2:+16490907 MS.gene060652:CDS
GGTTTAAAATTATAATCAGA+AGG 0.564252 2.2:+16488203 MS.gene060652:CDS
ACTCTCCTTCCCATTTGACA+TGG 0.564367 2.2:-16490836 None:intergenic
TAAACGTATCCCAGGTGGAA+TGG 0.564593 2.2:+16490400 MS.gene060652:intron
AGCTTCCGTGTCAAATGCGA+AGG 0.566653 2.2:+16490894 MS.gene060652:CDS
AGTCTCCTTCGCATTTGACA+CGG 0.580502 2.2:-16490899 None:intergenic
CTGTTTGGTCTCTGGCCATG+TGG 0.581097 2.2:-16490141 None:intergenic
AGCTGCCATGTCAAATGGGA+AGG 0.581376 2.2:+16490831 MS.gene060652:CDS
TGTGTATGATAACAGCAGCC+AGG 0.581859 2.2:+16490227 MS.gene060652:CDS
CAATGGAAGAGATACTATCA+AGG 0.583570 2.2:+16490439 MS.gene060652:CDS
AACCCCAGCTTTCTTTACCA+AGG 0.586825 2.2:-16490648 None:intergenic
AGCAATATTGTGAATCAACC+TGG 0.594625 2.2:-16488866 None:intergenic
AGCTTTAATTCAAGACCACA+TGG 0.613946 2.2:+16490126 MS.gene060652:CDS
TGTTACTTGATTCTTCATGA+GGG 0.616361 2.2:-16488804 None:intergenic
ATGAAGGTTTGATGATGGAG+AGG 0.619518 2.2:+16488833 MS.gene060652:CDS
TCCCAGGTGGAATGGTGCAG+CGG 0.626788 2.2:+16490408 MS.gene060652:intron
AAGCTGGGGTTGGAGCTGGA+TGG 0.631067 2.2:+16490660 MS.gene060652:CDS
CCCAAAACAAAGAAACTCAA+CGG 0.631804 2.2:+16490733 MS.gene060652:CDS
GGCTAAGATTAATGAGCTCG+AGG 0.640406 2.2:+16490288 MS.gene060652:CDS
ATGAGAGAAAGCATATGCCA+TGG 0.645984 2.2:-16490502 None:intergenic
AATCAAACGAGAGCGAGCCA+TGG 0.651456 2.2:+16490485 MS.gene060652:CDS
ATATTGTGAATCAACCTGGA+AGG 0.653405 2.2:-16488862 None:intergenic
CTTGAACCCGAACCTCCCAA+GGG 0.664743 2.2:-16490702 None:intergenic
AGAACATTTCAACATCTAAG+GGG 0.685065 2.2:+16490088 MS.gene060652:CDS
AGCCTTGGTAAAGAAAGCTG+GGG 0.725570 2.2:+16490646 MS.gene060652:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTTTTAAATCAAATTTAAA+TGG + chr2.2:16489608-16489627 MS.gene060652:intron 10.0%
!!! ATAATTTTAAACCATTTCTT+TGG - chr2.2:16488196-16488215 None:intergenic 15.0%
!!! TCAAATTTTGATTTTAAGAA+AGG - chr2.2:16490558-16490577 None:intergenic 15.0%
!!! TTTGCAAATTTTAAAGTTAA+AGG - chr2.2:16488292-16488311 None:intergenic 15.0%
!! AAAATTATCTGCCATATTAT+GGG - chr2.2:16489943-16489962 None:intergenic 20.0%
!! AAAATTCTATTTCCTTTAAG+TGG + chr2.2:16489335-16489354 MS.gene060652:intron 20.0%
!! ATAAAGTTCTATACTATCAA+AGG + chr2.2:16489473-16489492 MS.gene060652:intron 20.0%
!! TAAAATTATCTGCCATATTA+TGG - chr2.2:16489944-16489963 None:intergenic 20.0%
!! TGTGAAAATTGTAGTAAAAA+TGG - chr2.2:16488963-16488982 None:intergenic 20.0%
!!! CAAACTTAGTATTTTCATAA+GGG - chr2.2:16489286-16489305 None:intergenic 20.0%
!!! CACTTTTTTTTTATTACTAC+TGG + chr2.2:16489715-16489734 MS.gene060652:intron 20.0%
!!! GGTTTAAAATTATAATCAGA+AGG + chr2.2:16488203-16488222 MS.gene060652:CDS 20.0%
!!! GTTTTAAATATCCCATAATA+TGG + chr2.2:16489929-16489948 MS.gene060652:intron 20.0%
!!! TAATTAACTTGTTGTTAAGA+AGG - chr2.2:16489901-16489920 None:intergenic 20.0%
! AAATGCATTTCGTTCATTTA+TGG + chr2.2:16488903-16488922 MS.gene060652:CDS 25.0%
! AAATTTGATCACTAATGTCA+AGG + chr2.2:16490570-16490589 MS.gene060652:intron 25.0%
! AACAGAGATAACACTTTAAA+AGG - chr2.2:16490346-16490365 None:intergenic 25.0%
! AACTTAAAGCAATTATGTTC+AGG + chr2.2:16489747-16489766 MS.gene060652:intron 25.0%
! AAGAACATTTCAACATCTAA+GGG + chr2.2:16490087-16490106 MS.gene060652:CDS 25.0%
! AAGTATAGAAAAACAGATGA+GGG - chr2.2:16489877-16489896 None:intergenic 25.0%
! AAGTGTTATCTCTGTTATAT+TGG + chr2.2:16490350-16490369 MS.gene060652:intron 25.0%
! AGATAAATCAAACCAAGAAA+AGG + chr2.2:16488240-16488259 MS.gene060652:CDS 25.0%
! ATTGTCATAATACACATTTC+TGG + chr2.2:16488505-16488524 MS.gene060652:intron 25.0%
! CCTGAAAGAAAATCTAAATT+AGG - chr2.2:16488347-16488366 None:intergenic 25.0%
! TAAGTATAGAAAAACAGATG+AGG - chr2.2:16489878-16489897 None:intergenic 25.0%
! TTAAGAAAGGATAAATCAAG+TGG - chr2.2:16490545-16490564 None:intergenic 25.0%
! TTATAATCAGAAGGAAGAAA+CGG + chr2.2:16488212-16488231 MS.gene060652:CDS 25.0%
! TTTCCAACAGTTATAAAAGA+GGG - chr2.2:16489844-16489863 None:intergenic 25.0%
!! AAATCGACATTTCTAAAGAT+AGG - chr2.2:16489975-16489994 None:intergenic 25.0%
!! AACGAAAAGTACAAATTTGA+AGG + chr2.2:16488644-16488663 MS.gene060652:intron 25.0%
!! AATCGACATTTCTAAAGATA+GGG - chr2.2:16489974-16489993 None:intergenic 25.0%
!! ACGAAAAGTACAAATTTGAA+GGG + chr2.2:16488645-16488664 MS.gene060652:intron 25.0%
!! AGATGAGCTTTTTAATCTTA+CGG - chr2.2:16489094-16489113 None:intergenic 25.0%
!! CCTAATTTAGATTTTCTTTC+AGG + chr2.2:16488344-16488363 MS.gene060652:intron 25.0%
!! GAATATTTTGTATTTCCTCA+AGG + chr2.2:16489644-16489663 MS.gene060652:intron 25.0%
!! GCAAACTTAGTATTTTCATA+AGG - chr2.2:16489287-16489306 None:intergenic 25.0%
!! TTAGTCAATGGTGATTTTAA+TGG - chr2.2:16490811-16490830 None:intergenic 25.0%
!!! TTAGGGTTTTAATATGTTCT+TGG + chr2.2:16488424-16488443 MS.gene060652:intron 25.0%
!!! TTGATAATGACTTTAGAACA+TGG + chr2.2:16489678-16489697 MS.gene060652:intron 25.0%
!!! TTTCTGTTTTAGTTGCATTA+GGG + chr2.2:16488407-16488426 MS.gene060652:intron 25.0%
!!! TTTTCTGTTTTAGTTGCATT+AGG + chr2.2:16488406-16488425 MS.gene060652:intron 25.0%
AAAAAAAGTGAAGACACTAG+TGG - chr2.2:16489705-16489724 None:intergenic 30.0%
AAAAATCGCGAAATGCATAA+GGG - chr2.2:16489032-16489051 None:intergenic 30.0%
AACTAGACTATCACTAAAGA+AGG - chr2.2:16489592-16489611 None:intergenic 30.0%
AGAACATTTCAACATCTAAG+GGG + chr2.2:16490088-16490107 MS.gene060652:CDS 30.0%
CACTTAACTACATACATTCA+TGG + chr2.2:16490170-16490189 MS.gene060652:CDS 30.0%
CATGATTCCTTAACTACATA+GGG + chr2.2:16489207-16489226 MS.gene060652:intron 30.0%
CTTTCCAACAGTTATAAAAG+AGG - chr2.2:16489845-16489864 None:intergenic 30.0%
GAAGAACATTTCAACATCTA+AGG + chr2.2:16490086-16490105 MS.gene060652:CDS 30.0%
GGAAAGTCAGTTAAAAATTG+AGG + chr2.2:16490267-16490286 MS.gene060652:CDS 30.0%
GTGGTTCATCTTATCTAATT+TGG - chr2.2:16490773-16490792 None:intergenic 30.0%
TAAAAATCGCGAAATGCATA+AGG - chr2.2:16489033-16489052 None:intergenic 30.0%
TAGTTCAGAAAACCAAAGTT+AGG - chr2.2:16489117-16489136 None:intergenic 30.0%
TAGTTGACTAATGAGGATTT+GGG + chr2.2:16488369-16488388 MS.gene060652:intron 30.0%
TCAAAGTAAATGATACCTTG+AGG - chr2.2:16489662-16489681 None:intergenic 30.0%
TTAGTTGACTAATGAGGATT+TGG + chr2.2:16488368-16488387 MS.gene060652:intron 30.0%
TTTGTGTAAACTTGATCCAA+CGG + chr2.2:16489158-16489177 MS.gene060652:intron 30.0%
! AAGACATTTTTACCTTGCTT+TGG + chr2.2:16488702-16488721 MS.gene060652:intron 30.0%
! AAGTTTTTCACCACTACTTT+TGG - chr2.2:16488582-16488601 None:intergenic 30.0%
! AGACGAGTCATATATGTTTT+TGG - chr2.2:16488672-16488691 None:intergenic 30.0%
! ATCGACATTTCTAAAGATAG+GGG - chr2.2:16489973-16489992 None:intergenic 30.0%
! ATTTTTGCTTTAGTAGACGA+AGG - chr2.2:16489320-16489339 None:intergenic 30.0%
! CATGAGTTGACTAAAAAGTA+TGG + chr2.2:16488555-16488574 MS.gene060652:intron 30.0%
! GAAAGGAAAAAACCTTTTCT+TGG - chr2.2:16488255-16488274 None:intergenic 30.0%
! TAGTGGTGAAAAACTTTTGA+GGG + chr2.2:16488586-16488605 MS.gene060652:intron 30.0%
! TGTTACTTGATTCTTCATGA+GGG - chr2.2:16488807-16488826 None:intergenic 30.0%
! TTAGCCAATGGTGATTTTAA+TGG - chr2.2:16490874-16490893 None:intergenic 30.0%
! TTATGCATTTCGCGATTTTT+AGG + chr2.2:16489032-16489051 MS.gene060652:intron 30.0%
! TTTTCATAAGGGTTAAGCAT+AGG - chr2.2:16489275-16489294 None:intergenic 30.0%
!! ATGGTGATTTTGTTTCATTG+TGG - chr2.2:16490792-16490811 None:intergenic 30.0%
AAAAGCTCATCTCCTAACTT+TGG + chr2.2:16489102-16489121 MS.gene060652:intron 35.0%
AAAATCGCGAAATGCATAAG+GGG - chr2.2:16489031-16489050 None:intergenic 35.0%
AAAGTATGGACCAAAAGTAG+TGG + chr2.2:16488569-16488588 MS.gene060652:intron 35.0%
AAGAGAACAAGAAACAACAC+TGG - chr2.2:16488733-16488752 None:intergenic 35.0%
AGCAATATTGTGAATCAACC+TGG - chr2.2:16488869-16488888 None:intergenic 35.0%
ATATTGTGAATCAACCTGGA+AGG - chr2.2:16488865-16488884 None:intergenic 35.0%
ATCGCCATTAAAATCACCAT+TGG + chr2.2:16490867-16490886 MS.gene060652:CDS 35.0%
ATGTCAAGGTAATATTGCAG+TGG + chr2.2:16490584-16490603 MS.gene060652:intron 35.0%
CAACTTAACAAAGTGACATG+AGG + chr2.2:16489425-16489444 MS.gene060652:intron 35.0%
CAATGGAAGAGATACTATCA+AGG + chr2.2:16490439-16490458 MS.gene060652:CDS 35.0%
CAGGATTAAGCAAAAGAGAT+TGG + chr2.2:16490246-16490265 MS.gene060652:CDS 35.0%
CCATGATTCCTTAACTACAT+AGG + chr2.2:16489206-16489225 MS.gene060652:intron 35.0%
CCCAAAACAAAGAAACTCAA+CGG + chr2.2:16490733-16490752 MS.gene060652:CDS 35.0%
CCTATGTAGTTAAGGAATCA+TGG - chr2.2:16489209-16489228 None:intergenic 35.0%
CTAGATTCCCTATGTAGTTA+AGG - chr2.2:16489217-16489236 None:intergenic 35.0%
CTTAACTACATAGGGAATCT+AGG + chr2.2:16489215-16489234 MS.gene060652:intron 35.0%
TAACTATCACAGCCAGATTA+TGG - chr2.2:16490322-16490341 None:intergenic 35.0%
TCCATCATCAAACCTTCATT+GGG - chr2.2:16488832-16488851 None:intergenic 35.0%
TGATTGCAAGGAAAGTTAGA+AGG - chr2.2:16489363-16489382 None:intergenic 35.0%
TTCACAATATTGCTGCTACT+CGG + chr2.2:16488875-16488894 MS.gene060652:CDS 35.0%
TTTCCTTGCAATCATAGACA+TGG + chr2.2:16489369-16489388 MS.gene060652:intron 35.0%
TTTGATGCAATCATACGATC+TGG - chr2.2:16489185-16489204 None:intergenic 35.0%
! ACATTTTGTAAACGTATCCC+AGG + chr2.2:16490392-16490411 MS.gene060652:intron 35.0%
! AGCTTTAATTCAAGACCACA+TGG + chr2.2:16490126-16490145 MS.gene060652:CDS 35.0%
! AGTTAAGTGTTGTTCCTGTT+TGG - chr2.2:16490159-16490178 None:intergenic 35.0%
! CAATCTCTTTTGCTTAATCC+TGG - chr2.2:16490248-16490267 None:intergenic 35.0%
! CCAAAATACTGGTTACAGTT+TGG - chr2.2:16490613-16490632 None:intergenic 35.0%
! CCAAACTGTAACCAGTATTT+TGG + chr2.2:16490610-16490629 MS.gene060652:CDS 35.0%
! GTAGTGGTGAAAAACTTTTG+AGG + chr2.2:16488585-16488604 MS.gene060652:intron 35.0%
! TCACCCTCTTTTATAACTGT+TGG + chr2.2:16489838-16489857 MS.gene060652:intron 35.0%
!! AGTTAGAAGGTACCACTTAA+AGG - chr2.2:16489350-16489369 None:intergenic 35.0%
!! GTGTTACTTGATTCTTCATG+AGG - chr2.2:16488808-16488827 None:intergenic 35.0%
!! TCAGGATTAGTTGACTAATG+AGG + chr2.2:16488362-16488381 MS.gene060652:intron 35.0%
!!! CCGTTGAGTTTCTTTGTTTT+GGG - chr2.2:16490736-16490755 None:intergenic 35.0%
!!! CGTTGAGTTTCTTTGTTTTG+GGG - chr2.2:16490735-16490754 None:intergenic 35.0%
!!! GTTGAGTTTCTTTGTTTTGG+GGG - chr2.2:16490734-16490753 None:intergenic 35.0%
AATCTACTGAACCCTCCAAT+GGG + chr2.2:16488773-16488792 MS.gene060652:CDS 40.0%
ACTAAAGCTGCCATGTCAAA+TGG + chr2.2:16490826-16490845 MS.gene060652:CDS 40.0%
AGGACTGAAAAGAGAGAGAA+AGG - chr2.2:16488272-16488291 None:intergenic 40.0%
ATGAAGCCTGACCAAAATAC+TGG - chr2.2:16490624-16490643 None:intergenic 40.0%
CTAAAGCTGCCATGTCAAAT+GGG + chr2.2:16490827-16490846 MS.gene060652:CDS 40.0%
CTCCATCATCAAACCTTCAT+TGG - chr2.2:16488833-16488852 None:intergenic 40.0%
TCAAGGTAATATTGCAGTGG+AGG + chr2.2:16490587-16490606 MS.gene060652:intron 40.0%
TCAAGTAACACTCCCAATGA+AGG + chr2.2:16488817-16488836 MS.gene060652:CDS 40.0%
TCCCAATGAAGGTTTGATGA+TGG + chr2.2:16488828-16488847 MS.gene060652:CDS 40.0%
TCTCTTAGCACTCTTTGAAC+AGG + chr2.2:16488747-16488766 MS.gene060652:intron 40.0%
TGGAAGGGGAATTAAGTCTT+GGG + chr2.2:16489786-16489805 MS.gene060652:intron 40.0%
TTCTTCTTGCCTGCATACAT+AGG - chr2.2:16490072-16490091 None:intergenic 40.0%
TTGATTCTTCATGAGGGCTA+TGG - chr2.2:16488801-16488820 None:intergenic 40.0%
TTGCCTGCATACATAGGTAT+GGG - chr2.2:16490066-16490085 None:intergenic 40.0%
TTGGAAGGGGAATTAAGTCT+TGG + chr2.2:16489785-16489804 MS.gene060652:intron 40.0%
! ATAGCCTTGGTAAAGAAAGC+TGG + chr2.2:16490644-16490663 MS.gene060652:CDS 40.0%
! ATGAGAGAAAGCATATGCCA+TGG - chr2.2:16490505-16490524 None:intergenic 40.0%
! TAGCCTTGGTAAAGAAAGCT+GGG + chr2.2:16490645-16490664 MS.gene060652:CDS 40.0%
! TCATTGACTGCATGATTGCA+TGG - chr2.2:16489065-16489084 None:intergenic 40.0%
! TTTTGTAAACGTATCCCAGG+TGG + chr2.2:16490395-16490414 MS.gene060652:intron 40.0%
!! ACAGCTCTCTGATTTTGGAA+GGG + chr2.2:16489771-16489790 MS.gene060652:intron 40.0%
!! ATGAAGGTTTGATGATGGAG+AGG + chr2.2:16488833-16488852 MS.gene060652:CDS 40.0%
!! GCCGTTGAGTTTCTTTGTTT+TGG - chr2.2:16490737-16490756 None:intergenic 40.0%
!!! CTGTAACCAGTATTTTGGTC+AGG + chr2.2:16490615-16490634 MS.gene060652:CDS 40.0%
AACCCCAGCTTTCTTTACCA+AGG - chr2.2:16490651-16490670 None:intergenic 45.0%
AATCATACGATCTGGACCGT+TGG - chr2.2:16489177-16489196 None:intergenic 45.0%
AATGCGAAGGAGACTGAGAA+AGG + chr2.2:16490907-16490926 MS.gene060652:CDS 45.0%
ACTCTCCTTCCCATTTGACA+TGG - chr2.2:16490839-16490858 None:intergenic 45.0%
AGCCTTGGTAAAGAAAGCTG+GGG + chr2.2:16490646-16490665 MS.gene060652:CDS 45.0%
AGTCTCCTTCGCATTTGACA+CGG - chr2.2:16490902-16490921 None:intergenic 45.0%
CAATCTACTGAACCCTCCAA+TGG + chr2.2:16488772-16488791 MS.gene060652:CDS 45.0%
CAGCCATGTCTATGATTGCA+AGG - chr2.2:16489375-16489394 None:intergenic 45.0%
CTTGCCTGCATACATAGGTA+TGG - chr2.2:16490067-16490086 None:intergenic 45.0%
GAGGTAGTGTTGCCATAATC+TGG + chr2.2:16490307-16490326 MS.gene060652:intron 45.0%
GGCATATGCTTTCTCTCATC+AGG + chr2.2:16490506-16490525 MS.gene060652:CDS 45.0%
GGCTAAGATTAATGAGCTCG+AGG + chr2.2:16490288-16490307 MS.gene060652:CDS 45.0%
TAAACGTATCCCAGGTGGAA+TGG + chr2.2:16490400-16490419 MS.gene060652:intron 45.0%
TGCCTGCATACATAGGTATG+GGG - chr2.2:16490065-16490084 None:intergenic 45.0%
TGTGTATGATAACAGCAGCC+AGG + chr2.2:16490227-16490246 MS.gene060652:CDS 45.0%
TGTTGTTCCTGTTTGGTCTC+TGG - chr2.2:16490152-16490171 None:intergenic 45.0%
TTGGTAAAGAAAGCTGGGGT+TGG + chr2.2:16490650-16490669 MS.gene060652:CDS 45.0%
! CACAGCTCTCTGATTTTGGA+AGG + chr2.2:16489770-16489789 MS.gene060652:intron 45.0%
! CTAATTTGGTCTTCTGCTGC+TGG - chr2.2:16490759-16490778 None:intergenic 45.0%
! GACATGGCAGCTTTAGTCAA+TGG - chr2.2:16490823-16490842 None:intergenic 45.0%
!! CAGCTCTCTGATTTTGGAAG+GGG + chr2.2:16489772-16489791 MS.gene060652:intron 45.0%
!! TAATATACAAAAATAAAAAA+TGG + chr2.2:16489135-16489154 MS.gene060652:intron 5.0%
!!! TTAAAAGATTATAATATTTT+GGG + chr2.2:16488477-16488496 MS.gene060652:intron 5.0%
!!! TTTAAAAGATTATAATATTT+TGG + chr2.2:16488476-16488495 MS.gene060652:intron 5.0%
AATCAAACGAGAGCGAGCCA+TGG + chr2.2:16490485-16490504 MS.gene060652:CDS 50.0%
ACACTGGATGTGCCAAAGCA+AGG - chr2.2:16488717-16488736 None:intergenic 50.0%
AGCTGCCATGTCAAATGGGA+AGG + chr2.2:16490831-16490850 MS.gene060652:CDS 50.0%
AGCTTCCGTGTCAAATGCGA+AGG + chr2.2:16490894-16490913 MS.gene060652:CDS 50.0%
ATGAGGGCTATGGTTCCCAT+TGG - chr2.2:16488791-16488810 None:intergenic 50.0%
GCCTGCATACATAGGTATGG+GGG - chr2.2:16490064-16490083 None:intergenic 50.0%
GGTCAGGCTTCATATAGCCT+TGG + chr2.2:16490631-16490650 MS.gene060652:CDS 50.0%
TTGGAGCTGGATGGAACGTT+GGG + chr2.2:16490669-16490688 MS.gene060652:CDS 50.0%
! CAGGCACAGCTCTCTGATTT+TGG + chr2.2:16489766-16489785 MS.gene060652:intron 50.0%
! GACACGGAAGCTTTAGCCAA+TGG - chr2.2:16490886-16490905 None:intergenic 50.0%
!! GTGCAGCGGTTCTGAAACAA+TGG + chr2.2:16490422-16490441 MS.gene060652:CDS 50.0%
AAGAAAGCTGGGGTTGGAGC+TGG + chr2.2:16490656-16490675 MS.gene060652:CDS 55.0%
ACTTGAACCCGAACCTCCCA+AGG - chr2.2:16490706-16490725 None:intergenic 55.0%
AGGGCTATGGTTCCCATTGG+AGG - chr2.2:16488788-16488807 None:intergenic 55.0%
CACATGGCCAGAGACCAAAC+AGG + chr2.2:16490142-16490161 MS.gene060652:CDS 55.0%
CTGTTTGGTCTCTGGCCATG+TGG - chr2.2:16490144-16490163 None:intergenic 55.0%
CTTGAACCCGAACCTCCCAA+GGG - chr2.2:16490705-16490724 None:intergenic 55.0%
GCCCCCATACCTATGTATGC+AGG + chr2.2:16490060-16490079 MS.gene060652:intron 55.0%
GGGCTATGGTTCCCATTGGA+GGG - chr2.2:16488787-16488806 None:intergenic 55.0%
GTTGGAGCTGGATGGAACGT+TGG + chr2.2:16490668-16490687 MS.gene060652:CDS 55.0%
TGGAGAGGACAGTTCCTTCC+AGG + chr2.2:16488848-16488867 MS.gene060652:CDS 55.0%
AACCGCTGCACCATTCCACC+TGG - chr2.2:16490413-16490432 None:intergenic 60.0%
AAGCTGGGGTTGGAGCTGGA+TGG + chr2.2:16490660-16490679 MS.gene060652:CDS 60.0%
ACCGCTGCACCATTCCACCT+GGG - chr2.2:16490412-16490431 None:intergenic 60.0%
TCCCAGGTGGAATGGTGCAG+CGG + chr2.2:16490408-16490427 MS.gene060652:intron 60.0%
! TTGGGTTGCAGCGCGCCCTT+GGG + chr2.2:16490687-16490706 MS.gene060652:CDS 65.0%
AGCGCGCCCTTGGGAGGTTC+GGG + chr2.2:16490696-16490715 MS.gene060652:CDS 70.0%
CAGCGCGCCCTTGGGAGGTT+CGG + chr2.2:16490695-16490714 MS.gene060652:CDS 70.0%
! GTTGGGTTGCAGCGCGCCCT+TGG + chr2.2:16490686-16490705 MS.gene060652:CDS 70.0%
GGTTGCAGCGCGCCCTTGGG+AGG + chr2.2:16490690-16490709 MS.gene060652:CDS 75.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 16488187 16490948 16488187 ID=MS.gene060652
chr2.2 mRNA 16488187 16490948 16488187 ID=MS.gene060652.t1;Parent=MS.gene060652
chr2.2 exon 16488187 16488261 16488187 ID=MS.gene060652.t1.exon1;Parent=MS.gene060652.t1
chr2.2 CDS 16488187 16488261 16488187 ID=cds.MS.gene060652.t1;Parent=MS.gene060652.t1
chr2.2 exon 16488769 16488924 16488769 ID=MS.gene060652.t1.exon2;Parent=MS.gene060652.t1
chr2.2 CDS 16488769 16488924 16488769 ID=cds.MS.gene060652.t1;Parent=MS.gene060652.t1
chr2.2 exon 16490082 16490309 16490082 ID=MS.gene060652.t1.exon3;Parent=MS.gene060652.t1
chr2.2 CDS 16490082 16490309 16490082 ID=cds.MS.gene060652.t1;Parent=MS.gene060652.t1
chr2.2 exon 16490414 16490527 16490414 ID=MS.gene060652.t1.exon4;Parent=MS.gene060652.t1
chr2.2 CDS 16490414 16490527 16490414 ID=cds.MS.gene060652.t1;Parent=MS.gene060652.t1
chr2.2 exon 16490604 16490948 16490604 ID=MS.gene060652.t1.exon5;Parent=MS.gene060652.t1
chr2.2 CDS 16490604 16490948 16490604 ID=cds.MS.gene060652.t1;Parent=MS.gene060652.t1
Gene Sequence

>MS.gene060652

ATGGGTCCAAAGAAATGGTTTAAAATTATAATCAGAAGGAAGAAACGGAAAGAAGATAAATCAAACCAAGAAAAGGTTTTTTCCTTTCTCTCTCTTTTCAGTCCTTTAACTTTAAAATTTGCAAACATGATGAGAGTTTGCAGAGAATTTCTTTGCTCCTAATTTAGATTTTCTTTCAGGATTAGTTGACTAATGAGGATTTGGGTTTGTATAGTTCATTTTTCTGTTTTAGTTGCATTAGGGTTTTAATATGTTCTTGGTGTTTACAAATTCATATTTAATTTATGTTTTTAAAAGATTATAATATTTTGGGAAGAAATTGTCATAATACACATTTCTGGAAAATTTCATTAAAAAATATGAATAGACATGAGTTGACTAAAAAGTATGGACCAAAAGTAGTGGTGAAAAACTTTTGAGGGACTAAAAATCTTAACAAATTTTTTAGAAGAATTAAAACGAAAAGTACAAATTTGAAGGGACCAAAAACATATATGACTCGTCTCATTATGAGCAAGACATTTTTACCTTGCTTTGGCACATCCAGTGTTGTTTCTTGTTCTCTTAGCACTCTTTGAACAGGTGCAATCTACTGAACCCTCCAATGGGAACCATAGCCCTCATGAAGAATCAAGTAACACTCCCAATGAAGGTTTGATGATGGAGAGGACAGTTCCTTCCAGGTTGATTCACAATATTGCTGCTACTCGGATTCAAAATGCATTTCGTTCATTTATGGTACAATATCTTTGTGATGCTAGACTCTTTTTATTCCATTTTTACTACAATTTTCACAGTATTTGTGCTTGTAGCTTAGAACAAAATGTCAAAATTCATTATGCCCCTTATGCATTTCGCGATTTTTAGGTTTTTAACCATGCAATCATGCAGTCAATGATCTGAACCGTAAGATTAAAAAGCTCATCTCCTAACTTTGGTTTTCTGAACTAATATACAAAAATAAAAAATGGTTTGTGTAAACTTGATCCAACGGTCCAGATCGTATGATTGCATCAAATCCATGATTCCTTAACTACATAGGGAATCTAGGTCATGCACTTAGACTTTTGATTCTTATAATCAAACCTATGCTTAACCCTTATGAAAATACTAAGTTTGCTTTACACTCACCTTCGTCTACTAAAGCAAAAATTCTATTTCCTTTAAGTGGTACCTTCTAACTTTCCTTGCAATCATAGACATGGCTGCAACTGTCAACAATTCATTATTCTTTTTGTCAACTTAACAAAGTGACATGAGGTTATATTTTGATTCGTTAAAAGAAAATAAAGTTCTATACTATCAAAGGAAACATTATATTTTTATTTTTTTTACAATGAATGAAATACTTTGATATGATGTCTTTATTTGCAAAATCTAAAAATATAATAACTATCGACATCCTTCTTTAGTGATAGTCTAGTTTTAAATCAAATTTAAATGGAATAAATGACACTGAATATTTTGTATTTCCTCAAGGTATCATTTACTTTGATAATGACTTTAGAACATGGACCACTAGTGTCTTCACTTTTTTTTTATTACTACTGGAAAGAATTAAACTTAAAGCAATTATGTTCAGGCACAGCTCTCTGATTTTGGAAGGGGAATTAAGTCTTGGGTTATGATGATATTCTCTGTTATGCAGTTATCACCCTCTTTTATAACTGTTGGAAAGCAATTATTTCCCTCATCTGTTTTTCTATACTTACCTTCTTAACAACAAGTTAATTAATTTTTTAGTTTTAAATATCCCATAATATGGCAGATAATTTTATACATGCCCCTATCTTTAGAAATGTCGATTTGAAGCTTTTCTACAAAATTTAGCAATTTTCACATAAGCTTAATGACATGATGAAACTTACTACTAGCCCCCATACCTATGTATGCAGGCAAGAAGAACATTTCAACATCTAAGGGGAGCAGAGAACTTTGAAGCTTTAATTCAAGACCACATGGCCAGAGACCAAACAGGAACAACACTTAACTACATACATTCATGGAGCAGAATACAAGATCAAATTAGAGCTCGTAGAATGTGTATGATAACAGCAGCCAGGATTAAGCAAAAGAGATTGGAAAGTCAGTTAAAAATTGAGGCTAAGATTAATGAGCTCGAGGTAGTGTTGCCATAATCTGGCTGTGATAGTTAGCCTTTTAAAGTGTTATCTCTGTTATATTGGTCATTTTCAATAAAGTGTGACATTTTGTAAACGTATCCCAGGTGGAATGGTGCAGCGGTTCTGAAACAATGGAAGAGATACTATCAAGGATACATCAGCGAGAAGAAGCAGCAATCAAACGAGAGCGAGCCATGGCATATGCTTTCTCTCATCAGGTGCATAACTTGTTCCACTTGATTTATCCTTTCTTAAAATCAAAATTTGATCACTAATGTCAAGGTAATATTGCAGTGGAGGCCAAACTGTAACCAGTATTTTGGTCAGGCTTCATATAGCCTTGGTAAAGAAAGCTGGGGTTGGAGCTGGATGGAACGTTGGGTTGCAGCGCGCCCTTGGGAGGTTCGGGTTCAAGTTCAGTCCCCCAAAACAAAGAAACTCAACGGCCAGCAGCAGAAGACCAAATTAGATAAGATGAACCACAATGAAACAAAATCACCATTAAAATCACCATTGACTAAAGCTGCCATGTCAAATGGGAAGGAGAGTGAAACAAAATCGCCATTAAAATCACCATTGGCTAAAGCTTCCGTGTCAAATGCGAAGGAGACTGAGAAAGGAAAGAAAAACAAAACATGA
Protein sequence

>MS.gene060652.t1

MGPKKWFKIIIRRKKRKEDKSNQEKVQSTEPSNGNHSPHEESSNTPNEGLMMERTVPSRLIHNIAATRIQNAFRSFMARRTFQHLRGAENFEALIQDHMARDQTGTTLNYIHSWSRIQDQIRARRMCMITAARIKQKRLESQLKIEAKINELEVEWCSGSETMEEILSRIHQREEAAIKRERAMAYAFSHQWRPNCNQYFGQASYSLGKESWGWSWMERWVAARPWEVRVQVQSPKTKKLNGQQQKTKLDKMNHNETKSPLKSPLTKAAMSNGKESETKSPLKSPLAKASVSNAKETEKGKKNKT