Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71246.t1 | XP_013464663.1 | 92.4 | 119 | 6 | 1 | 1 | 119 | 1 | 116 | 1.40E-50 | 208.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71246.t1 | A0A072VB72 | 92.4 | 119 | 6 | 1 | 1 | 119 | 1 | 116 | 1.0e-50 | 208.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05474 | MS.gene71246 | 0.821008 | 5.07E-53 | -1.69E-46 |
| MS.gene059776 | MS.gene71246 | 0.994047 | 3.67E-204 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71246.t1 | MTR_2g078780 | 92.437 | 119 | 6 | 1 | 1 | 119 | 1 | 116 | 1.50e-76 | 222 |
| MS.gene71246.t1 | MTR_1g061895 | 93.939 | 99 | 6 | 0 | 1 | 99 | 1 | 99 | 4.04e-63 | 187 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGTGAAAGAAAAAAAAATTG+AGG | + | 14449:5722-5741 | MS.gene71246:intergenic | 20.0% |
| !!! | TTTTTTTTTTAAATTCAGGG+TGG | - | 14449:5805-5824 | MS.gene71246:CDS | 20.0% |
| ! | AACAAAGAAAACTGTACAAT+TGG | - | 14449:5930-5949 | MS.gene71246:intron | 25.0% |
| AGAAGTAGTGAAGATTTATC+AGG | - | 14449:6009-6028 | MS.gene71246:CDS | 30.0% | |
| CATTAGGAGCAATAAATCAA+GGG | - | 14449:5833-5852 | MS.gene71246:intron | 30.0% | |
| GAAGTAGTGAAGATTTATCA+GGG | - | 14449:6010-6029 | MS.gene71246:CDS | 30.0% | |
| CCATTAGGAGCAATAAATCA+AGG | - | 14449:5832-5851 | MS.gene71246:intron | 35.0% | |
| CCTTGATTTATTGCTCCTAA+TGG | + | 14449:5835-5854 | MS.gene71246:intergenic | 35.0% | |
| !! | AGAAGTTGGTAAGGAAAATG+TGG | - | 14449:5891-5910 | MS.gene71246:intron | 35.0% |
| !! | GTTGATAACTTTAGCAGCAT+TGG | + | 14449:5658-5677 | MS.gene71246:intergenic | 35.0% |
| AGGAAAATGTGGAAACTGCA+TGG | - | 14449:5902-5921 | MS.gene71246:intron | 40.0% | |
| GAAGATTTATCAGGGCAACT+TGG | - | 14449:6018-6037 | MS.gene71246:CDS | 40.0% | |
| GGAAAATGTGGAAACTGCAT+GGG | - | 14449:5903-5922 | MS.gene71246:intron | 40.0% | |
| TCTTTGCACTGAGCAAGAAA+GGG | + | 14449:5618-5637 | MS.gene71246:intergenic | 40.0% | |
| TGAGGCTGATATGAATGTTG+TGG | - | 14449:5972-5991 | MS.gene71246:CDS | 40.0% | |
| !! | AACTTTAGCAGCATTGGTAG+TGG | + | 14449:5652-5671 | MS.gene71246:intergenic | 40.0% |
| !! | CTAGAGAGTGTTGAAGAAGT+TGG | - | 14449:5877-5896 | MS.gene71246:intron | 40.0% |
| !! | GAGTGTTGAAGAAGTTGGTA+AGG | - | 14449:5882-5901 | MS.gene71246:intron | 40.0% |
| AACTTGGAGATGGCTGTGAT+AGG | - | 14449:6034-6053 | MS.gene71246:CDS | 45.0% | |
| ATTCAGGGTGGTGATCCATT+AGG | - | 14449:5817-5836 | MS.gene71246:CDS | 45.0% | |
| ATTCATATCAGCCTCAGCAG+TGG | + | 14449:5968-5987 | MS.gene71246:intergenic | 45.0% | |
| GTCTTTGCACTGAGCAAGAA+AGG | + | 14449:5619-5638 | MS.gene71246:intergenic | 45.0% | |
| TCTTGCTCAGTGCAAAGACT+TGG | - | 14449:5620-5639 | MS.gene71246:CDS | 45.0% | |
| TTATCAGGGCAACTTGGAGA+TGG | - | 14449:6024-6043 | MS.gene71246:CDS | 45.0% | |
| !!! | TTTTTTTTTTTTTAAATTCA+GGG | - | 14449:5802-5821 | MS.gene71246:CDS | 5.0% |
| !!! | TTTTTTTTTTTTTTAAATTC+AGG | - | 14449:5801-5820 | MS.gene71246:CDS | 5.0% |
| AGGGTCAGAGAAAGCAAGAG+AGG | - | 14449:5852-5871 | MS.gene71246:intron | 50.0% | |
| GACTGAGACAACCACTGCTG+AGG | - | 14449:5954-5973 | MS.gene71246:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 14449 | gene | 5600 | 6068 | 5600 | ID=MS.gene71246 |
| 14449 | mRNA | 5600 | 6068 | 5600 | ID=MS.gene71246.t1;Parent=MS.gene71246 |
| 14449 | exon | 5955 | 6068 | 5955 | ID=MS.gene71246.t1.exon1;Parent=MS.gene71246.t1 |
| 14449 | CDS | 5955 | 6068 | 5955 | ID=cds.MS.gene71246.t1;Parent=MS.gene71246.t1 |
| 14449 | exon | 5600 | 5845 | 5600 | ID=MS.gene71246.t1.exon2;Parent=MS.gene71246.t1 |
| 14449 | CDS | 5600 | 5845 | 5600 | ID=cds.MS.gene71246.t1;Parent=MS.gene71246.t1 |
| Gene Sequence |
| Protein sequence |
