Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71278.t1 | XP_013464663.1 | 92.4 | 119 | 6 | 1 | 1 | 119 | 1 | 116 | 1.40E-50 | 208.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71278.t1 | A0A072VB72 | 92.4 | 119 | 6 | 1 | 1 | 119 | 1 | 116 | 1.0e-50 | 208.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05474 | MS.gene71278 | 0.806581 | 7.67E-50 | -1.69E-46 |
| MS.gene059776 | MS.gene71278 | 0.99255 | 5.71E-194 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71278.t1 | MTR_2g078780 | 92.437 | 119 | 6 | 1 | 1 | 119 | 1 | 116 | 1.50e-76 | 222 |
| MS.gene71278.t1 | MTR_1g061895 | 93.939 | 99 | 6 | 0 | 1 | 99 | 1 | 99 | 4.04e-63 | 187 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGTGAAAGAAAAAAAAATTG+AGG | - | 14448:4785-4804 | MS.gene71278:intergenic | 20.0% |
| !!! | TTTTTTTTTTAAATTCAGGG+TGG | + | 14448:4868-4887 | MS.gene71278:intron | 20.0% |
| ! | AACAAAGAAAACTGTACAAT+TGG | + | 14448:4993-5012 | MS.gene71278:CDS | 25.0% |
| AGAAGTAGTGAAGATTTATC+AGG | + | 14448:5072-5091 | MS.gene71278:CDS | 30.0% | |
| CATTAGGAGCAATAAATCAA+GGG | + | 14448:4896-4915 | MS.gene71278:CDS | 30.0% | |
| GAAGTAGTGAAGATTTATCA+GGG | + | 14448:5073-5092 | MS.gene71278:CDS | 30.0% | |
| CCATTAGGAGCAATAAATCA+AGG | + | 14448:4895-4914 | MS.gene71278:CDS | 35.0% | |
| CCTTGATTTATTGCTCCTAA+TGG | - | 14448:4898-4917 | MS.gene71278:intergenic | 35.0% | |
| !! | AGAAGTTGGTAAGGAAAATG+TGG | + | 14448:4954-4973 | MS.gene71278:CDS | 35.0% |
| !! | GTTGATAACTTTAGCAGCAT+TGG | - | 14448:4721-4740 | MS.gene71278:intergenic | 35.0% |
| AGGAAAATGTGGAAACTGCA+TGG | + | 14448:4965-4984 | MS.gene71278:CDS | 40.0% | |
| GAAGATTTATCAGGGCAACT+TGG | + | 14448:5081-5100 | MS.gene71278:CDS | 40.0% | |
| GGAAAATGTGGAAACTGCAT+GGG | + | 14448:4966-4985 | MS.gene71278:CDS | 40.0% | |
| TCTTTGCACTGAGCAAGAAA+GGG | - | 14448:4681-4700 | MS.gene71278:intergenic | 40.0% | |
| TGAGGCTGATATGAATGTTG+TGG | + | 14448:5035-5054 | MS.gene71278:CDS | 40.0% | |
| !! | AACTTTAGCAGCATTGGTAG+TGG | - | 14448:4715-4734 | MS.gene71278:intergenic | 40.0% |
| !! | CTAGAGAGTGTTGAAGAAGT+TGG | + | 14448:4940-4959 | MS.gene71278:CDS | 40.0% |
| !! | GAGTGTTGAAGAAGTTGGTA+AGG | + | 14448:4945-4964 | MS.gene71278:CDS | 40.0% |
| AACTTGGAGATGGCTGTGAT+AGG | + | 14448:5097-5116 | MS.gene71278:CDS | 45.0% | |
| ATTCAGGGTGGTGATCCATT+AGG | + | 14448:4880-4899 | MS.gene71278:intron | 45.0% | |
| ATTCATATCAGCCTCAGCAG+TGG | - | 14448:5031-5050 | MS.gene71278:intergenic | 45.0% | |
| GTCTTTGCACTGAGCAAGAA+AGG | - | 14448:4682-4701 | MS.gene71278:intergenic | 45.0% | |
| TCTTGCTCAGTGCAAAGACT+TGG | + | 14448:4683-4702 | MS.gene71278:CDS | 45.0% | |
| TTATCAGGGCAACTTGGAGA+TGG | + | 14448:5087-5106 | MS.gene71278:CDS | 45.0% | |
| !!! | TTTTTTTTTTTTTAAATTCA+GGG | + | 14448:4865-4884 | MS.gene71278:intron | 5.0% |
| !!! | TTTTTTTTTTTTTTAAATTC+AGG | + | 14448:4864-4883 | MS.gene71278:intron | 5.0% |
| AGGGTCAGAGAAAGCAAGAG+AGG | + | 14448:4915-4934 | MS.gene71278:CDS | 50.0% | |
| GACTGAGACAACCACTGCTG+AGG | + | 14448:5017-5036 | MS.gene71278:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 14448 | gene | 4663 | 5131 | 4663 | ID=MS.gene71278 |
| 14448 | mRNA | 4663 | 5131 | 4663 | ID=MS.gene71278.t1;Parent=MS.gene71278 |
| 14448 | exon | 4663 | 4776 | 4663 | ID=MS.gene71278.t1.exon1;Parent=MS.gene71278.t1 |
| 14448 | CDS | 4663 | 4776 | 4663 | ID=cds.MS.gene71278.t1;Parent=MS.gene71278.t1 |
| 14448 | exon | 4886 | 5131 | 4886 | ID=MS.gene71278.t1.exon2;Parent=MS.gene71278.t1 |
| 14448 | CDS | 4886 | 5131 | 4886 | ID=cds.MS.gene71278.t1;Parent=MS.gene71278.t1 |
| Gene Sequence |
| Protein sequence |
