Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71488.t1 | XP_003588667.1 | 99.5 | 184 | 1 | 0 | 1 | 184 | 1 | 184 | 1.10E-94 | 355.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71488.t1 | Q6NQH9 | 38.5 | 182 | 93 | 4 | 2 | 181 | 1 | 165 | 7.2e-18 | 92.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71488.t1 | G7I5S9 | 99.5 | 184 | 1 | 0 | 1 | 184 | 1 | 184 | 8.0e-95 | 355.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050379 | MS.gene71488 | 0.812687 | 3.74E-51 | -1.69E-46 |
| MS.gene057174 | MS.gene71488 | 0.803144 | 4.01E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71488.t1 | MTR_1g011280 | 99.457 | 184 | 1 | 0 | 1 | 184 | 1 | 184 | 2.12e-135 | 376 |
| MS.gene71488.t1 | MTR_1g011280 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 4.05e-98 | 280 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71488.t1 | AT5G25540 | 39.306 | 173 | 86 | 5 | 2 | 172 | 1 | 156 | 1.48e-24 | 94.7 |
| MS.gene71488.t1 | AT5G25540 | 39.306 | 173 | 86 | 5 | 2 | 172 | 1 | 156 | 1.48e-24 | 94.7 |
| MS.gene71488.t1 | AT5G11440 | 38.172 | 186 | 69 | 7 | 2 | 181 | 1 | 146 | 9.05e-24 | 92.0 |
| MS.gene71488.t1 | AT5G11440 | 38.172 | 186 | 69 | 7 | 2 | 181 | 1 | 146 | 9.05e-24 | 92.0 |
| MS.gene71488.t1 | AT5G25540 | 37.956 | 137 | 71 | 3 | 2 | 136 | 1 | 125 | 7.01e-18 | 76.3 |
Find 40 sgRNAs with CRISPR-Local
Find 96 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATATTGATTTGGAATATCTT+AGG | 0.249147 | 1.3:-9623232 | MS.gene71488:CDS |
| GCAGAATCAGCTGGCATAAC+AGG | 0.299732 | 1.3:+9621984 | None:intergenic |
| GAAGATTCCAAGGACTATAA+TGG | 0.336500 | 1.3:-9623425 | MS.gene71488:CDS |
| TGAAGAGCTTGATATTGATT+TGG | 0.356150 | 1.3:-9623243 | MS.gene71488:CDS |
| TCTAACATTAGTGTCCCTTT+TGG | 0.366851 | 1.3:+9623460 | None:intergenic |
| CTTCCAGAGTCGCTGGATAT+TGG | 0.369329 | 1.3:-9622020 | MS.gene71488:CDS |
| TATCTTAGGATGACATTTCC+TGG | 0.384113 | 1.3:-9623218 | MS.gene71488:CDS |
| AAGATTCCAAGGACTATAAT+GGG | 0.425801 | 1.3:-9623424 | MS.gene71488:CDS |
| GCTGACAGAGAATCAGTATA+TGG | 0.429347 | 1.3:-9623267 | MS.gene71488:CDS |
| GACAAGTTGCACATCATTTG+TGG | 0.436093 | 1.3:+9623373 | None:intergenic |
| TACAGTCCCATTATAGTCCT+TGG | 0.442789 | 1.3:+9623418 | None:intergenic |
| GGAACGTACGCAGCTGCATA+TGG | 0.448700 | 1.3:+9623488 | None:intergenic |
| CGTACGTTCCTATGGCCAAA+AGG | 0.452015 | 1.3:-9623475 | MS.gene71488:CDS |
| CAATGTAAACAGTGGTGACT+TGG | 0.452279 | 1.3:-9623165 | MS.gene71488:CDS |
| AACGCTCAAAGGCTCTCTCC+GGG | 0.456586 | 1.3:-9623344 | MS.gene71488:CDS |
| GAACGCTCAAAGGCTCTCTC+CGG | 0.458973 | 1.3:-9623345 | MS.gene71488:CDS |
| ACATCATTTGTGGTATATTG+AGG | 0.475780 | 1.3:+9623383 | None:intergenic |
| TCACCAATATCCAGCGACTC+TGG | 0.486657 | 1.3:+9622017 | None:intergenic |
| TGCAGCTGCGTACGTTCCTA+TGG | 0.491027 | 1.3:-9623483 | MS.gene71488:CDS |
| AGGACTATAATGGGACTGTA+TGG | 0.501746 | 1.3:-9623415 | MS.gene71488:CDS |
| TTTAAGATTATACCTCAAGT+TGG | 0.519886 | 1.3:+9623120 | None:intergenic |
| TGGAAGTCTTCCAGAGTCGC+TGG | 0.520324 | 1.3:-9622027 | MS.gene71488:CDS |
| AAACAGAAGAATGCAACAGC+TGG | 0.520914 | 1.3:-9621954 | MS.gene71488:CDS |
| CAAGTGATGAGATGAAGTAC+TGG | 0.526656 | 1.3:+9621929 | None:intergenic |
| GAAAAGTAAGAGCTCGAAGC+AGG | 0.531344 | 1.3:+9623311 | None:intergenic |
| TTTGATGAAGCAGAATCAGC+TGG | 0.546972 | 1.3:+9621975 | None:intergenic |
| AGTAAGAGCTCGAAGCAGGC+TGG | 0.559139 | 1.3:+9623315 | None:intergenic |
| GTACGTTCCTATGGCCAAAA+GGG | 0.559981 | 1.3:-9623474 | MS.gene71488:CDS |
| GGATTCAAAGAAGACTTTCG+CGG | 0.573737 | 1.3:+9623509 | None:intergenic |
| GATGATGCTGTTGACGCTCA+TGG | 0.575449 | 1.3:-9622047 | MS.gene71488:CDS |
| TTGTCGAGAAGAACGCTCAA+AGG | 0.582696 | 1.3:-9623355 | MS.gene71488:CDS |
| TTACATTGTAGACATCTACA+AGG | 0.595095 | 1.3:+9623180 | None:intergenic |
| TCATTTGTGGTATATTGAGG+AGG | 0.614473 | 1.3:+9623386 | None:intergenic |
| TTATGTGACAGAAGATTCCA+AGG | 0.616516 | 1.3:-9623435 | MS.gene71488:CDS |
| GTCAGCTGTGCCACACTCTG+TGG | 0.634116 | 1.3:+9623284 | None:intergenic |
| TGAGATGCTAAGCCAACTTG+AGG | 0.661491 | 1.3:-9623132 | MS.gene71488:intron |
| TACATTGTAGACATCTACAA+GGG | 0.670208 | 1.3:+9623181 | None:intergenic |
| AGGGACTCATCAGATATACC+AGG | 0.671666 | 1.3:+9623200 | None:intergenic |
| ACGCTCAAAGGCTCTCTCCG+GGG | 0.694097 | 1.3:-9623343 | MS.gene71488:CDS |
| GATGTCTACAATGTAAACAG+TGG | 0.761880 | 1.3:-9623173 | MS.gene71488:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATATAATTGTAACTATCA+AGG | - | chr1.3:9622623-9622642 | MS.gene71488:intron | 15.0% |
| !! | ATATAAATATCAAGACAATA+TGG | + | chr1.3:9622885-9622904 | None:intergenic | 15.0% |
| !!! | ATCTTAAAATTATTAGCTTT+TGG | - | chr1.3:9622339-9622358 | MS.gene71488:intron | 15.0% |
| !!! | TCTTAAAATTATTAGCTTTT+GGG | - | chr1.3:9622340-9622359 | MS.gene71488:intron | 15.0% |
| !! | AAAAACAAACTCTCAAAAAT+TGG | + | chr1.3:9623362-9623381 | None:intergenic | 20.0% |
| !! | TGATTGGAATATATGAAAAA+AGG | + | chr1.3:9622391-9622410 | None:intergenic | 20.0% |
| !! | TTATATATGCAGATGAAAAT+AGG | - | chr1.3:9623140-9623159 | MS.gene71488:CDS | 20.0% |
| !! | TTATATTTATAAATCCCGAA+TGG | + | chr1.3:9622611-9622630 | None:intergenic | 20.0% |
| !!! | ATATTGATTTGGAATATCTT+AGG | - | chr1.3:9622212-9622231 | MS.gene71488:intron | 20.0% |
| ! | AAAACAAACATAACAGGAAA+TGG | + | chr1.3:9622923-9622942 | None:intergenic | 25.0% |
| ! | AAACAAACATAACAGGAAAT+GGG | + | chr1.3:9622922-9622941 | None:intergenic | 25.0% |
| ! | AGTCTTAAAACAAACATAAC+AGG | + | chr1.3:9622929-9622948 | None:intergenic | 25.0% |
| ! | ATTAACTGGAAATTATGGTT+AGG | - | chr1.3:9622669-9622688 | MS.gene71488:intron | 25.0% |
| ! | CACAAATTAACTGGAAATTA+TGG | - | chr1.3:9622664-9622683 | MS.gene71488:intron | 25.0% |
| ! | TACTCTCATAATCATGTTAA+TGG | - | chr1.3:9623306-9623325 | MS.gene71488:CDS | 25.0% |
| !! | CATAATTTCCAGTTAATTTG+TGG | + | chr1.3:9622666-9622685 | None:intergenic | 25.0% |
| !! | CTGATTTGAATCTAGTATAT+AGG | - | chr1.3:9623087-9623106 | MS.gene71488:intron | 25.0% |
| !! | TTTAAGATTATACCTCAAGT+TGG | + | chr1.3:9622327-9622346 | None:intergenic | 25.0% |
| !!! | ACATGTTCAGTTTTTGTTTT+AGG | - | chr1.3:9623035-9623054 | MS.gene71488:intron | 25.0% |
| !!! | GTTATGTTTGTTTTAAGACT+TGG | - | chr1.3:9622929-9622948 | MS.gene71488:intron | 25.0% |
| AAGATTCCAAGGACTATAAT+GGG | - | chr1.3:9622020-9622039 | MS.gene71488:CDS | 30.0% | |
| AATAAGAAGTTATCATCTCG+TGG | - | chr1.3:9622793-9622812 | MS.gene71488:intron | 30.0% | |
| ACATCATTTGTGGTATATTG+AGG | + | chr1.3:9622064-9622083 | None:intergenic | 30.0% | |
| ATAAAAGTCACTGAACTGAT+TGG | + | chr1.3:9622508-9622527 | None:intergenic | 30.0% | |
| ATCATATGCCACAAATTAAC+TGG | - | chr1.3:9622655-9622674 | MS.gene71488:intron | 30.0% | |
| ATTACAGTCACTTAACTGAT+TGG | + | chr1.3:9622407-9622426 | None:intergenic | 30.0% | |
| GACTGTAATTGTAACTATCA+AGG | - | chr1.3:9622418-9622437 | MS.gene71488:intron | 30.0% | |
| TACATTGTAGACATCTACAA+GGG | + | chr1.3:9622266-9622285 | None:intergenic | 30.0% | |
| TGACTAGACTTTCATACATA+TGG | + | chr1.3:9622587-9622606 | None:intergenic | 30.0% | |
| TTACATTGTAGACATCTACA+AGG | + | chr1.3:9622267-9622286 | None:intergenic | 30.0% | |
| TTAGGATGTGTAGTTAAATG+TGG | - | chr1.3:9622687-9622706 | MS.gene71488:intron | 30.0% | |
| TTTGACTGGAAAATTTGGTT+AGG | - | chr1.3:9622464-9622483 | MS.gene71488:intron | 30.0% | |
| ! | ACTTCTTTGCTGATTAATGT+TGG | - | chr1.3:9623187-9623206 | MS.gene71488:CDS | 30.0% |
| ! | TCAGTTCAGTGACTTTTATT+CGG | - | chr1.3:9622509-9622528 | MS.gene71488:intron | 30.0% |
| ! | TGAAGAGCTTGATATTGATT+TGG | - | chr1.3:9622201-9622220 | MS.gene71488:intron | 30.0% |
| ! | TTCGATTTGACTGGAAAATT+TGG | - | chr1.3:9622459-9622478 | MS.gene71488:intron | 30.0% |
| !!! | TGTTCAGTTTTTGTTTTAGG+TGG | - | chr1.3:9623038-9623057 | MS.gene71488:intron | 30.0% |
| !!! | TTTAAGACTTGGTGTTTTTC+TGG | - | chr1.3:9622940-9622959 | MS.gene71488:intron | 30.0% |
| AGACTATCTTCAGATGACAT+TGG | - | chr1.3:9622766-9622785 | MS.gene71488:intron | 35.0% | |
| CTGGAAAATTTGGTTAGGTT+AGG | - | chr1.3:9622469-9622488 | MS.gene71488:intron | 35.0% | |
| GAAGATTCCAAGGACTATAA+TGG | - | chr1.3:9622019-9622038 | MS.gene71488:CDS | 35.0% | |
| GATGTCTACAATGTAAACAG+TGG | - | chr1.3:9622271-9622290 | MS.gene71488:intron | 35.0% | |
| TATCTTAGGATGACATTTCC+TGG | - | chr1.3:9622226-9622245 | MS.gene71488:intron | 35.0% | |
| TCATTTGTGGTATATTGAGG+AGG | + | chr1.3:9622061-9622080 | None:intergenic | 35.0% | |
| TGAAAGTCTAGTCAACCATT+CGG | - | chr1.3:9622593-9622612 | MS.gene71488:intron | 35.0% | |
| TGTCATCTGAAGATAGTCTT+CGG | + | chr1.3:9622765-9622784 | None:intergenic | 35.0% | |
| TTATGTGACAGAAGATTCCA+AGG | - | chr1.3:9622009-9622028 | MS.gene71488:CDS | 35.0% | |
| TTTGCGTAGAGAGAAAACTT+TGG | - | chr1.3:9623278-9623297 | MS.gene71488:CDS | 35.0% | |
| ! | AACCACAAAGCATTACACAT+GGG | + | chr1.3:9622986-9623005 | None:intergenic | 35.0% |
| ! | ATCATATGCTTCGATTTGAC+TGG | - | chr1.3:9622450-9622469 | MS.gene71488:intron | 35.0% |
| ! | CAGTTCAGTGACTTTTATTC+GGG | - | chr1.3:9622510-9622529 | MS.gene71488:intron | 35.0% |
| ! | GAAAAGAGTATACACAGGAA+GGG | + | chr1.3:9622737-9622756 | None:intergenic | 35.0% |
| ! | TCTAACATTAGTGTCCCTTT+TGG | + | chr1.3:9621987-9622006 | None:intergenic | 35.0% |
| !!! | CAGTTTTTGTTTTAGGTGGA+AGG | - | chr1.3:9623042-9623061 | MS.gene71488:intron | 35.0% |
| AAACAGAAGAATGCAACAGC+TGG | - | chr1.3:9623490-9623509 | MS.gene71488:CDS | 40.0% | |
| CAAGTGATGAGATGAAGTAC+TGG | + | chr1.3:9623518-9623537 | None:intergenic | 40.0% | |
| CAATGTAAACAGTGGTGACT+TGG | - | chr1.3:9622279-9622298 | MS.gene71488:intron | 40.0% | |
| GAAAGTCTAGTCAACCATTC+GGG | - | chr1.3:9622594-9622613 | MS.gene71488:intron | 40.0% | |
| GACAAGTTGCACATCATTTG+TGG | + | chr1.3:9622074-9622093 | None:intergenic | 40.0% | |
| GCTGACAGAGAATCAGTATA+TGG | - | chr1.3:9622177-9622196 | MS.gene71488:intron | 40.0% | |
| GGATTCAAAGAAGACTTTCG+CGG | + | chr1.3:9621938-9621957 | None:intergenic | 40.0% | |
| GTCATCTGAAGATAGTCTTC+GGG | + | chr1.3:9622764-9622783 | None:intergenic | 40.0% | |
| TACAGTCCCATTATAGTCCT+TGG | + | chr1.3:9622029-9622048 | None:intergenic | 40.0% | |
| TTCCCATGTGTAATGCTTTG+TGG | - | chr1.3:9622981-9623000 | MS.gene71488:intron | 40.0% | |
| TTTGATGAAGCAGAATCAGC+TGG | + | chr1.3:9623472-9623491 | None:intergenic | 40.0% | |
| ! | AGGACTATAATGGGACTGTA+TGG | - | chr1.3:9622029-9622048 | MS.gene71488:CDS | 40.0% |
| ! | GAACCACAAAGCATTACACA+TGG | + | chr1.3:9622987-9623006 | None:intergenic | 40.0% |
| ! | GAACGGAAAAGAGTATACAC+AGG | + | chr1.3:9622742-9622761 | None:intergenic | 40.0% |
| ! | GGAAAAGAGTATACACAGGA+AGG | + | chr1.3:9622738-9622757 | None:intergenic | 40.0% |
| AGGGACTCATCAGATATACC+AGG | + | chr1.3:9622247-9622266 | None:intergenic | 45.0% | |
| CGTGGTTGTTGAATCTCACA+GGG | - | chr1.3:9622811-9622830 | MS.gene71488:intron | 45.0% | |
| CTGAAGATAGTCTTCGGGAA+CGG | + | chr1.3:9622759-9622778 | None:intergenic | 45.0% | |
| GAAAAGTAAGAGCTCGAAGC+AGG | + | chr1.3:9622136-9622155 | None:intergenic | 45.0% | |
| GTACGTTCCTATGGCCAAAA+GGG | - | chr1.3:9621970-9621989 | MS.gene71488:CDS | 45.0% | |
| TCGTGGTTGTTGAATCTCAC+AGG | - | chr1.3:9622810-9622829 | MS.gene71488:intron | 45.0% | |
| TGAGATGCTAAGCCAACTTG+AGG | - | chr1.3:9622312-9622331 | MS.gene71488:intron | 45.0% | |
| TTGGTCGTTCATTCAGTTGC+TGG | - | chr1.3:9623206-9623225 | MS.gene71488:CDS | 45.0% | |
| TTGTCGAGAAGAACGCTCAA+AGG | - | chr1.3:9622089-9622108 | MS.gene71488:intron | 45.0% | |
| ! | CTTTTCATCACCACAGAGTG+TGG | - | chr1.3:9622150-9622169 | MS.gene71488:intron | 45.0% |
| ! | TTAGTGTCCCTTTTGGCCAT+AGG | + | chr1.3:9621980-9621999 | None:intergenic | 45.0% |
| CGTACGTTCCTATGGCCAAA+AGG | - | chr1.3:9621969-9621988 | MS.gene71488:CDS | 50.0% | |
| CTTCCAGAGTCGCTGGATAT+TGG | - | chr1.3:9623424-9623443 | MS.gene71488:CDS | 50.0% | |
| GATGATGCTGTTGACGCTCA+TGG | - | chr1.3:9623397-9623416 | MS.gene71488:CDS | 50.0% | |
| GCAGAATCAGCTGGCATAAC+AGG | + | chr1.3:9623463-9623482 | None:intergenic | 50.0% | |
| TCACCAATATCCAGCGACTC+TGG | + | chr1.3:9623430-9623449 | None:intergenic | 50.0% | |
| TGTTGAATCTCACAGGGTGC+TGG | - | chr1.3:9622817-9622836 | MS.gene71488:intron | 50.0% | |
| AACGCTCAAAGGCTCTCTCC+GGG | - | chr1.3:9622100-9622119 | MS.gene71488:intron | 55.0% | |
| AGTAAGAGCTCGAAGCAGGC+TGG | + | chr1.3:9622132-9622151 | None:intergenic | 55.0% | |
| GAACGCTCAAAGGCTCTCTC+CGG | - | chr1.3:9622099-9622118 | MS.gene71488:intron | 55.0% | |
| GGAACGTACGCAGCTGCATA+TGG | + | chr1.3:9621959-9621978 | None:intergenic | 55.0% | |
| TGCAGCTGCGTACGTTCCTA+TGG | - | chr1.3:9621961-9621980 | MS.gene71488:CDS | 55.0% | |
| TGGAAGTCTTCCAGAGTCGC+TGG | - | chr1.3:9623417-9623436 | MS.gene71488:CDS | 55.0% | |
| !! | AATCTCACAGGGTGCTGGCT+AGG | - | chr1.3:9622822-9622841 | MS.gene71488:intron | 55.0% |
| !!! | GAAGCAGGCTGGTTTTTCCC+CGG | + | chr1.3:9622121-9622140 | None:intergenic | 55.0% |
| ACGCTCAAAGGCTCTCTCCG+GGG | - | chr1.3:9622101-9622120 | MS.gene71488:intron | 60.0% | |
| GTCAGCTGTGCCACACTCTG+TGG | + | chr1.3:9622163-9622182 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 9621926 | 9623540 | 9621926 | ID=MS.gene71488 |
| chr1.3 | mRNA | 9621926 | 9623540 | 9621926 | ID=MS.gene71488.t1;Parent=MS.gene71488 |
| chr1.3 | exon | 9623133 | 9623540 | 9623133 | ID=MS.gene71488.t1.exon1;Parent=MS.gene71488.t1 |
| chr1.3 | CDS | 9623133 | 9623540 | 9623133 | ID=cds.MS.gene71488.t1;Parent=MS.gene71488.t1 |
| chr1.3 | exon | 9621926 | 9622072 | 9621926 | ID=MS.gene71488.t1.exon2;Parent=MS.gene71488.t1 |
| chr1.3 | CDS | 9621926 | 9622072 | 9621926 | ID=cds.MS.gene71488.t1;Parent=MS.gene71488.t1 |
| Gene Sequence |
| Protein sequence |
