Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71640.t1 | XP_013470006.1 | 99.1 | 213 | 2 | 0 | 1 | 213 | 1 | 213 | 1.60E-108 | 402.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71640.t1 | O64628 | 77.9 | 208 | 45 | 1 | 6 | 213 | 5 | 211 | 2.7e-85 | 316.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71640.t1 | B7FMC0 | 99.1 | 213 | 2 | 0 | 1 | 213 | 1 | 213 | 1.1e-108 | 402.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene71640 | MS.gene91284 | PPI |
| MS.gene71640 | MS.gene76830 | PPI |
| MS.gene71640 | MS.gene060292 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71640.t1 | MTR_1g106955 | 99.061 | 213 | 2 | 0 | 1 | 213 | 1 | 213 | 7.28e-156 | 430 |
| MS.gene71640.t1 | MTR_8g076890 | 42.675 | 157 | 88 | 2 | 25 | 180 | 105 | 260 | 1.81e-38 | 134 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71640.t1 | AT2G18990 | 77.885 | 208 | 45 | 1 | 6 | 213 | 5 | 211 | 1.36e-119 | 338 |
| MS.gene71640.t1 | AT3G25580 | 77.404 | 208 | 45 | 2 | 6 | 213 | 5 | 210 | 1.32e-118 | 336 |
| MS.gene71640.t1 | AT3G25580 | 77.073 | 205 | 45 | 2 | 9 | 213 | 3 | 205 | 1.25e-116 | 330 |
| MS.gene71640.t1 | AT5G66410 | 36.585 | 205 | 123 | 4 | 7 | 209 | 28 | 227 | 1.05e-38 | 133 |
| MS.gene71640.t1 | AT3G50960 | 42.000 | 150 | 85 | 2 | 23 | 171 | 40 | 188 | 3.95e-37 | 129 |
| MS.gene71640.t1 | AT3G50960 | 42.000 | 150 | 85 | 2 | 23 | 171 | 40 | 188 | 3.95e-37 | 129 |
Find 44 sgRNAs with CRISPR-Local
Find 78 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGACAAGCATTTAAGTATTT+TGG | 0.171906 | 1.4:+82483570 | MS.gene71640:CDS |
| TGACTTTAGCACCGAGGTTT+TGG | 0.292143 | 1.4:+82483883 | MS.gene71640:CDS |
| GTCATTTCTTCCGCGAAAAT+TGG | 0.299383 | 1.4:+82483050 | MS.gene71640:CDS |
| CGAAATTGTGCGAACCTTGC+AGG | 0.328390 | 1.4:-82483074 | None:intergenic |
| GCTCTGCCGAACACTTCTTT+TGG | 0.354451 | 1.4:-82483984 | None:intergenic |
| TCAATACACCGTGCAAGGTC+CGG | 0.384757 | 1.4:+82483953 | MS.gene71640:CDS |
| GCTGCAACAAATGAAGAAAA+TGG | 0.402598 | 1.4:+82482922 | MS.gene71640:CDS |
| CTTGATTCTCGATCACCTCT+TGG | 0.414878 | 1.4:-82482799 | None:intergenic |
| CAATACACCGTGCAAGGTCC+GGG | 0.429480 | 1.4:+82483954 | MS.gene71640:CDS |
| TTGCAGCCTCCTTTCTCGTA+GGG | 0.482545 | 1.4:-82482906 | None:intergenic |
| TTTGCGCCCCGGACCTTGCA+CGG | 0.490890 | 1.4:-82483961 | None:intergenic |
| GTCTTTCTCTGACGGAATCT+CGG | 0.495284 | 1.4:-82482990 | None:intergenic |
| GTTGCAGCCTCCTTTCTCGT+AGG | 0.496830 | 1.4:-82482907 | None:intergenic |
| ATCTTGAAGCCCTACGAGAA+AGG | 0.502488 | 1.4:+82482897 | MS.gene71640:CDS |
| CGCAAACCAAAAGAAGTGTT+CGG | 0.506657 | 1.4:+82483978 | MS.gene71640:CDS |
| CGGTGTAATCTCCGTGTCCG+AGG | 0.513907 | 1.4:-82482970 | None:intergenic |
| CGTCATCGTCGAGACGTTCA+AGG | 0.517999 | 1.4:-82482874 | None:intergenic |
| CGAAGCAGCATATTGAGACT+CGG | 0.534134 | 1.4:+82483593 | MS.gene71640:CDS |
| TTCTTTATGAGAGCAATAGT+AGG | 0.546329 | 1.4:-82483679 | None:intergenic |
| GACGGTGGCGCAAGCAGTCG+AGG | 0.556072 | 1.4:+82482826 | MS.gene71640:CDS |
| CCGCGAAAATTGGCCCTGCA+AGG | 0.557879 | 1.4:+82483060 | MS.gene71640:CDS |
| TACCAAAGTGGATGATTATG+TGG | 0.558275 | 1.4:+82483702 | MS.gene71640:CDS |
| GAAATTGTGCGAACCTTGCA+GGG | 0.562815 | 1.4:-82483073 | None:intergenic |
| GTCATCGTCGAGACGTTCAA+GGG | 0.565214 | 1.4:-82482873 | None:intergenic |
| GAGAAGAAGTCTTTCTCTGA+CGG | 0.577836 | 1.4:-82482998 | None:intergenic |
| AATCGTCAATACACCGTGCA+AGG | 0.582115 | 1.4:+82483948 | MS.gene71640:CDS |
| CGTTGGATCTCCCTCGGACA+CGG | 0.582223 | 1.4:+82482959 | MS.gene71640:CDS |
| TACCACATAATCATCCACTT+TGG | 0.582640 | 1.4:-82483704 | None:intergenic |
| CGTAGCCGTTGGATCTCCCT+CGG | 0.582964 | 1.4:+82482953 | MS.gene71640:CDS |
| TGGCGGAGAAGCGTAGCCGT+TGG | 0.601854 | 1.4:+82482942 | MS.gene71640:CDS |
| CAATCTTTCTTCCAAAACCT+CGG | 0.602383 | 1.4:-82483894 | None:intergenic |
| ACGATCACTTGCTTTAACGA+CGG | 0.607682 | 1.4:-82483020 | None:intergenic |
| GGTGTAATCTCCGTGTCCGA+GGG | 0.608291 | 1.4:-82482969 | None:intergenic |
| TACAAGTTACTCAGAATCTG+AGG | 0.610014 | 1.4:-82484020 | None:intergenic |
| CGTGTCCGAGGGAGATCCAA+CGG | 0.633581 | 1.4:-82482958 | None:intergenic |
| AATACACCGTGCAAGGTCCG+GGG | 0.653754 | 1.4:+82483955 | MS.gene71640:CDS |
| GATCGAGAATCAAGTACTGA+CGG | 0.657827 | 1.4:+82482808 | MS.gene71640:CDS |
| TCTCATAAAGAATACCAAAG+TGG | 0.658073 | 1.4:+82483690 | MS.gene71640:CDS |
| TTGAAGCCCTACGAGAAAGG+AGG | 0.672465 | 1.4:+82482900 | MS.gene71640:CDS |
| TACTGATGACTTTAGCACCG+AGG | 0.705317 | 1.4:+82483877 | MS.gene71640:CDS |
| GCAACAAATGAAGAAAATGG+CGG | 0.709368 | 1.4:+82482925 | MS.gene71640:CDS |
| AAGTGTTCGGCAGAGCACAA+CGG | 0.717985 | 1.4:+82483991 | MS.gene71640:CDS |
| GGACAAAGGAAAGATCCAAG+AGG | 0.724397 | 1.4:+82482784 | MS.gene71640:CDS |
| CGAGAATCAAGTACTGACGG+TGG | 0.737424 | 1.4:+82482811 | MS.gene71640:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAACTTTATAAAAATATTAT+TGG | + | chr1.4:82483326-82483345 | MS.gene71640:intron | 10.0% |
| !!! | AAGAATTTTAATTTAAACGT+AGG | + | chr1.4:82483304-82483323 | MS.gene71640:intron | 15.0% |
| !! | ACATGTGATTACATAATTAT+CGG | + | chr1.4:82483190-82483209 | MS.gene71640:intron | 20.0% |
| !! | TATTGACATGAATTCTATAT+TGG | + | chr1.4:82483766-82483785 | MS.gene71640:intron | 20.0% |
| ! | AATTATGTAATCACATGTGT+AGG | - | chr1.4:82483188-82483207 | None:intergenic | 25.0% |
| ! | ATATAAAAACTGTAAGTCTG+CGG | - | chr1.4:82483495-82483514 | None:intergenic | 25.0% |
| ! | TACTAACATCAAGATGATAA+TGG | + | chr1.4:82483819-82483838 | MS.gene71640:intron | 25.0% |
| ! | TAGAATTATCTGTCTATCAT+TGG | + | chr1.4:82483421-82483440 | MS.gene71640:intron | 25.0% |
| !!! | ATATTTTTTTCGCTGAAATG+AGG | + | chr1.4:82483511-82483530 | MS.gene71640:intron | 25.0% |
| !!! | ATGATAATGGTATGTGTTTT+AGG | + | chr1.4:82483832-82483851 | MS.gene71640:intron | 25.0% |
| AATATTATTGGCAGTGCATA+CGG | + | chr1.4:82483338-82483357 | MS.gene71640:intron | 30.0% | |
| AATTATCTGTCTATCATTGG+AGG | + | chr1.4:82483424-82483443 | MS.gene71640:intron | 30.0% | |
| ACATGAATTCTATATTGGAC+AGG | + | chr1.4:82483771-82483790 | MS.gene71640:intron | 30.0% | |
| ATTATCTGTCTATCATTGGA+GGG | + | chr1.4:82483425-82483444 | MS.gene71640:intron | 30.0% | |
| ATTATGTGGTATGTGCTTAA+AGG | + | chr1.4:82483716-82483735 | MS.gene71640:intron | 30.0% | |
| CATGAATTCTATATTGGACA+GGG | + | chr1.4:82483772-82483791 | MS.gene71640:intron | 30.0% | |
| CGGAATTCAAATTCAAATTG+TGG | - | chr1.4:82483475-82483494 | None:intergenic | 30.0% | |
| TCTCATAAAGAATACCAAAG+TGG | + | chr1.4:82483690-82483709 | MS.gene71640:CDS | 30.0% | |
| TGATTACATAATTATCGGTG+TGG | + | chr1.4:82483195-82483214 | MS.gene71640:intron | 30.0% | |
| TTCTTTATGAGAGCAATAGT+AGG | - | chr1.4:82483682-82483701 | None:intergenic | 30.0% | |
| ! | GGACAAGCATTTAAGTATTT+TGG | + | chr1.4:82483570-82483589 | MS.gene71640:CDS | 30.0% |
| ! | TAAAGCTCAAGTCATCTTTT+TGG | + | chr1.4:82483919-82483938 | MS.gene71640:CDS | 30.0% |
| !! | ATAATGGTATGTGTTTTAGG+TGG | + | chr1.4:82483835-82483854 | MS.gene71640:intron | 30.0% |
| !! | TAATGGTATGTGTTTTAGGT+GGG | + | chr1.4:82483836-82483855 | MS.gene71640:intron | 30.0% |
| ATATTATTGGCAGTGCATAC+GGG | + | chr1.4:82483339-82483358 | MS.gene71640:intron | 35.0% | |
| CAATCTTTCTTCCAAAACCT+CGG | - | chr1.4:82483897-82483916 | None:intergenic | 35.0% | |
| GCAACAAATGAAGAAAATGG+CGG | + | chr1.4:82482925-82482944 | MS.gene71640:CDS | 35.0% | |
| GCTGCAACAAATGAAGAAAA+TGG | + | chr1.4:82482922-82482941 | MS.gene71640:CDS | 35.0% | |
| TACCAAAGTGGATGATTATG+TGG | + | chr1.4:82483702-82483721 | MS.gene71640:CDS | 35.0% | |
| TACCACATAATCATCCACTT+TGG | - | chr1.4:82483707-82483726 | None:intergenic | 35.0% | |
| TTGAGCTTCTCAGCTAAAAA+TGG | - | chr1.4:82483646-82483665 | None:intergenic | 35.0% | |
| ACGATCACTTGCTTTAACGA+CGG | - | chr1.4:82483023-82483042 | None:intergenic | 40.0% | |
| GAGAAGAAGTCTTTCTCTGA+CGG | - | chr1.4:82483001-82483020 | None:intergenic | 40.0% | |
| GTAATCACATGTGTAGGTGT+CGG | - | chr1.4:82483182-82483201 | None:intergenic | 40.0% | |
| GTCATTTCTTCCGCGAAAAT+TGG | + | chr1.4:82483050-82483069 | MS.gene71640:CDS | 40.0% | |
| ! | AGCTCAAGTCATCTTTTTGG+AGG | + | chr1.4:82483922-82483941 | MS.gene71640:CDS | 40.0% |
| ! | GCTCAAGTCATCTTTTTGGA+GGG | + | chr1.4:82483923-82483942 | MS.gene71640:CDS | 40.0% |
| ! | TGCATTCAGTTTGCTTAGCT+TGG | + | chr1.4:82483276-82483295 | MS.gene71640:intron | 40.0% |
| !! | CGCAAACCAAAAGAAGTGTT+CGG | + | chr1.4:82483978-82483997 | MS.gene71640:CDS | 40.0% |
| !! | GATCGAGAATCAAGTACTGA+CGG | + | chr1.4:82482808-82482827 | MS.gene71640:CDS | 40.0% |
| !! | TAGGTGGGTTTTGATGAACT+TGG | + | chr1.4:82483851-82483870 | MS.gene71640:intron | 40.0% |
| !!! | CGAGGTTTTGGAAGAAAGAT+TGG | + | chr1.4:82483895-82483914 | MS.gene71640:CDS | 40.0% |
| AATCGTCAATACACCGTGCA+AGG | + | chr1.4:82483948-82483967 | MS.gene71640:CDS | 45.0% | |
| ATCTTGAAGCCCTACGAGAA+AGG | + | chr1.4:82482897-82482916 | MS.gene71640:CDS | 45.0% | |
| CACAGTTCATTCACAGGTTG+TGG | + | chr1.4:82483549-82483568 | MS.gene71640:intron | 45.0% | |
| CGAAGCAGCATATTGAGACT+CGG | + | chr1.4:82483593-82483612 | MS.gene71640:CDS | 45.0% | |
| CTTGATTCTCGATCACCTCT+TGG | - | chr1.4:82482802-82482821 | None:intergenic | 45.0% | |
| GAAATTGTGCGAACCTTGCA+GGG | - | chr1.4:82483076-82483095 | None:intergenic | 45.0% | |
| GGACAAAGGAAAGATCCAAG+AGG | + | chr1.4:82482784-82482803 | MS.gene71640:CDS | 45.0% | |
| GTCTTTCTCTGACGGAATCT+CGG | - | chr1.4:82482993-82483012 | None:intergenic | 45.0% | |
| TACTGATGACTTTAGCACCG+AGG | + | chr1.4:82483877-82483896 | MS.gene71640:CDS | 45.0% | |
| TGTGCTCACAGTTCATTCAC+AGG | + | chr1.4:82483543-82483562 | MS.gene71640:intron | 45.0% | |
| !! | GTGGGTTTTGATGAACTTGG+TGG | + | chr1.4:82483854-82483873 | MS.gene71640:CDS | 45.0% |
| !! | TGACTTTAGCACCGAGGTTT+TGG | + | chr1.4:82483883-82483902 | MS.gene71640:CDS | 45.0% |
| AAGTGTTCGGCAGAGCACAA+CGG | + | chr1.4:82483991-82484010 | MS.gene71640:CDS | 50.0% | |
| CGAAATTGTGCGAACCTTGC+AGG | - | chr1.4:82483077-82483096 | None:intergenic | 50.0% | |
| CGGTGTGGATGTGTAGTGTT+AGG | + | chr1.4:82483210-82483229 | MS.gene71640:intron | 50.0% | |
| GTCATCGTCGAGACGTTCAA+GGG | - | chr1.4:82482876-82482895 | None:intergenic | 50.0% | |
| TCAATACACCGTGCAAGGTC+CGG | + | chr1.4:82483953-82483972 | MS.gene71640:CDS | 50.0% | |
| TTGAAGCCCTACGAGAAAGG+AGG | + | chr1.4:82482900-82482919 | MS.gene71640:CDS | 50.0% | |
| TTGCAGCCTCCTTTCTCGTA+GGG | - | chr1.4:82482909-82482928 | None:intergenic | 50.0% | |
| ! | GCTCTGCCGAACACTTCTTT+TGG | - | chr1.4:82483987-82484006 | None:intergenic | 50.0% |
| !! | CGAGAATCAAGTACTGACGG+TGG | + | chr1.4:82482811-82482830 | MS.gene71640:CDS | 50.0% |
| !!! | ACTTCTTTTGGTTTGCGCCC+CGG | - | chr1.4:82483975-82483994 | None:intergenic | 50.0% |
| AATACACCGTGCAAGGTCCG+GGG | + | chr1.4:82483955-82483974 | MS.gene71640:CDS | 55.0% | |
| CAATACACCGTGCAAGGTCC+GGG | + | chr1.4:82483954-82483973 | MS.gene71640:CDS | 55.0% | |
| CGTCATCGTCGAGACGTTCA+AGG | - | chr1.4:82482877-82482896 | None:intergenic | 55.0% | |
| GGTGTAATCTCCGTGTCCGA+GGG | - | chr1.4:82482972-82482991 | None:intergenic | 55.0% | |
| GTTGCAGCCTCCTTTCTCGT+AGG | - | chr1.4:82482910-82482929 | None:intergenic | 55.0% | |
| ! | CCTTGCAGGGCCAATTTTCG+CGG | - | chr1.4:82483063-82483082 | None:intergenic | 55.0% |
| CCGCGAAAATTGGCCCTGCA+AGG | + | chr1.4:82483060-82483079 | MS.gene71640:CDS | 60.0% | |
| CGGTGTAATCTCCGTGTCCG+AGG | - | chr1.4:82482973-82482992 | None:intergenic | 60.0% | |
| CGTAGCCGTTGGATCTCCCT+CGG | + | chr1.4:82482953-82482972 | MS.gene71640:CDS | 60.0% | |
| CGTGTCCGAGGGAGATCCAA+CGG | - | chr1.4:82482961-82482980 | None:intergenic | 60.0% | |
| CGTTGGATCTCCCTCGGACA+CGG | + | chr1.4:82482959-82482978 | MS.gene71640:CDS | 60.0% | |
| TTTGCGCCCCGGACCTTGCA+CGG | - | chr1.4:82483964-82483983 | None:intergenic | 65.0% | |
| ! | TGGCGGAGAAGCGTAGCCGT+TGG | + | chr1.4:82482942-82482961 | MS.gene71640:CDS | 65.0% |
| GACGGTGGCGCAAGCAGTCG+AGG | + | chr1.4:82482826-82482845 | MS.gene71640:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 82482782 | 82484036 | 82482782 | ID=MS.gene71640 |
| chr1.4 | mRNA | 82482782 | 82484036 | 82482782 | ID=MS.gene71640.t1;Parent=MS.gene71640 |
| chr1.4 | exon | 82482782 | 82483081 | 82482782 | ID=MS.gene71640.t1.exon1;Parent=MS.gene71640.t1 |
| chr1.4 | CDS | 82482782 | 82483081 | 82482782 | ID=cds.MS.gene71640.t1;Parent=MS.gene71640.t1 |
| chr1.4 | exon | 82483565 | 82483723 | 82483565 | ID=MS.gene71640.t1.exon2;Parent=MS.gene71640.t1 |
| chr1.4 | CDS | 82483565 | 82483723 | 82483565 | ID=cds.MS.gene71640.t1;Parent=MS.gene71640.t1 |
| chr1.4 | exon | 82483854 | 82484036 | 82483854 | ID=MS.gene71640.t1.exon3;Parent=MS.gene71640.t1 |
| chr1.4 | CDS | 82483854 | 82484036 | 82483854 | ID=cds.MS.gene71640.t1;Parent=MS.gene71640.t1 |
| Gene Sequence |
| Protein sequence |
