Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91284.t1 | XP_013470006.1 | 99.1 | 213 | 2 | 0 | 1 | 213 | 1 | 213 | 1.60E-108 | 402.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91284.t1 | O64628 | 77.9 | 208 | 45 | 1 | 6 | 213 | 5 | 211 | 2.7e-85 | 316.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91284.t1 | B7FMC0 | 99.1 | 213 | 2 | 0 | 1 | 213 | 1 | 213 | 1.1e-108 | 402.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene060292 | MS.gene91284 | PPI |
| MS.gene71640 | MS.gene91284 | PPI |
| MS.gene91284 | MS.gene76830 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91284.t1 | MTR_1g106955 | 99.061 | 213 | 2 | 0 | 1 | 213 | 1 | 213 | 7.28e-156 | 430 |
| MS.gene91284.t1 | MTR_8g076890 | 42.675 | 157 | 88 | 2 | 25 | 180 | 105 | 260 | 1.81e-38 | 134 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91284.t1 | AT2G18990 | 77.885 | 208 | 45 | 1 | 6 | 213 | 5 | 211 | 1.36e-119 | 338 |
| MS.gene91284.t1 | AT3G25580 | 77.404 | 208 | 45 | 2 | 6 | 213 | 5 | 210 | 1.32e-118 | 336 |
| MS.gene91284.t1 | AT3G25580 | 77.073 | 205 | 45 | 2 | 9 | 213 | 3 | 205 | 1.25e-116 | 330 |
| MS.gene91284.t1 | AT5G66410 | 36.585 | 205 | 123 | 4 | 7 | 209 | 28 | 227 | 1.05e-38 | 133 |
| MS.gene91284.t1 | AT3G50960 | 42.000 | 150 | 85 | 2 | 23 | 171 | 40 | 188 | 3.95e-37 | 129 |
| MS.gene91284.t1 | AT3G50960 | 42.000 | 150 | 85 | 2 | 23 | 171 | 40 | 188 | 3.95e-37 | 129 |
Find 44 sgRNAs with CRISPR-Local
Find 77 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGACAAGCATTTAAGTATTT+TGG | 0.171906 | 1.3:+73229349 | MS.gene91284:CDS |
| GTTGCAGCCTCCTTTCTCTT+AGG | 0.207376 | 1.3:-73228685 | None:intergenic |
| TGACTTTAGCACCGAGGTTT+TGG | 0.292143 | 1.3:+73229662 | MS.gene91284:CDS |
| GTCATTTCTTCCGCGAAAAT+TGG | 0.299383 | 1.3:+73228828 | MS.gene91284:CDS |
| TTGCAGCCTCCTTTCTCTTA+GGG | 0.338559 | 1.3:-73228684 | None:intergenic |
| GCTCTGCCGAACACTTCTTT+TGG | 0.354451 | 1.3:-73229763 | None:intergenic |
| AGAAATTGTGCGAACCTTGC+AGG | 0.367889 | 1.3:-73228852 | None:intergenic |
| TCAATACACCGTGCAAGGTC+CGG | 0.384757 | 1.3:+73229732 | MS.gene91284:CDS |
| GCTGCAACAAATGAAGAAAA+TGG | 0.402598 | 1.3:+73228700 | MS.gene91284:CDS |
| CTTGATTCTCGATCACCTCT+TGG | 0.414878 | 1.3:-73228577 | None:intergenic |
| CAATACACCGTGCAAGGTCC+GGG | 0.429480 | 1.3:+73229733 | MS.gene91284:CDS |
| TTTGCGCCCCGGACCTTGCA+CGG | 0.490890 | 1.3:-73229740 | None:intergenic |
| GTCTTTCTCTGACGGAATCT+CGG | 0.495284 | 1.3:-73228768 | None:intergenic |
| TTCTTTATGAGAGCAATAGT+TGG | 0.506238 | 1.3:-73229458 | None:intergenic |
| CGCAAACCAAAAGAAGTGTT+CGG | 0.506657 | 1.3:+73229757 | MS.gene91284:CDS |
| CGGTGTAATCTCCGTGTCCG+AGG | 0.513907 | 1.3:-73228748 | None:intergenic |
| CGTCATCGTCGAGACGTTCA+AGG | 0.517999 | 1.3:-73228652 | None:intergenic |
| ATCTTGAAGCCCTAAGAGAA+AGG | 0.527404 | 1.3:+73228675 | MS.gene91284:CDS |
| CGAAGCAGCATATTGAGACT+CGG | 0.534134 | 1.3:+73229372 | MS.gene91284:CDS |
| GAAATTGTGCGAACCTTGCA+GGG | 0.544550 | 1.3:-73228851 | None:intergenic |
| GACGGTGGCGCAAGCAGTCG+AGG | 0.556072 | 1.3:+73228604 | MS.gene91284:CDS |
| CCGCGAAAATTGGCCCTGCA+AGG | 0.557879 | 1.3:+73228838 | MS.gene91284:CDS |
| TACCAAAGTGGATGATTATG+TGG | 0.558275 | 1.3:+73229481 | MS.gene91284:CDS |
| GTCATCGTCGAGACGTTCAA+GGG | 0.565214 | 1.3:-73228651 | None:intergenic |
| GAGAAGAAGTCTTTCTCTGA+CGG | 0.577836 | 1.3:-73228776 | None:intergenic |
| AATCGTCAATACACCGTGCA+AGG | 0.582115 | 1.3:+73229727 | MS.gene91284:CDS |
| CGTTGGATCTCCCTCGGACA+CGG | 0.582223 | 1.3:+73228737 | MS.gene91284:CDS |
| TACCACATAATCATCCACTT+TGG | 0.582640 | 1.3:-73229483 | None:intergenic |
| CGTAGCCGTTGGATCTCCCT+CGG | 0.582964 | 1.3:+73228731 | MS.gene91284:CDS |
| TGGCGGAGAAGCGTAGCCGT+TGG | 0.601854 | 1.3:+73228720 | MS.gene91284:CDS |
| CAATCTTTCTTCCAAAACCT+CGG | 0.602383 | 1.3:-73229673 | None:intergenic |
| ACGATCACTTGCTTTAACGA+CGG | 0.607682 | 1.3:-73228798 | None:intergenic |
| GGTGTAATCTCCGTGTCCGA+GGG | 0.608291 | 1.3:-73228747 | None:intergenic |
| TACAAGTTACTCAGAATCTG+AGG | 0.610014 | 1.3:-73229799 | None:intergenic |
| CGTGTCCGAGGGAGATCCAA+CGG | 0.633581 | 1.3:-73228736 | None:intergenic |
| AATACACCGTGCAAGGTCCG+GGG | 0.653754 | 1.3:+73229734 | MS.gene91284:CDS |
| GATCGAGAATCAAGTACTGA+CGG | 0.657827 | 1.3:+73228586 | MS.gene91284:CDS |
| TCTCATAAAGAATACCAAAG+TGG | 0.658073 | 1.3:+73229469 | MS.gene91284:CDS |
| TTGAAGCCCTAAGAGAAAGG+AGG | 0.672465 | 1.3:+73228678 | MS.gene91284:CDS |
| TACTGATGACTTTAGCACCG+AGG | 0.705317 | 1.3:+73229656 | MS.gene91284:CDS |
| GCAACAAATGAAGAAAATGG+CGG | 0.709368 | 1.3:+73228703 | MS.gene91284:CDS |
| AAGTGTTCGGCAGAGCACAA+CGG | 0.717985 | 1.3:+73229770 | MS.gene91284:CDS |
| GGACAAAGGAAAGATCCAAG+AGG | 0.724397 | 1.3:+73228562 | MS.gene91284:CDS |
| CGAGAATCAAGTACTGACGG+TGG | 0.737424 | 1.3:+73228589 | MS.gene91284:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAACTTTATAAAAATATTAT+TGG | + | chr1.3:73229104-73229123 | MS.gene91284:intron | 10.0% |
| !!! | AAGAATTTTAATTTAAACGT+AGG | + | chr1.3:73229082-73229101 | MS.gene91284:intron | 15.0% |
| !! | ACATGTGATTACATAATTAT+CGG | + | chr1.3:73228968-73228987 | MS.gene91284:intron | 20.0% |
| !! | TAGAATTATCTGTTTATCAT+TGG | + | chr1.3:73229199-73229218 | MS.gene91284:intron | 20.0% |
| !! | TATTGACATGAATTCTATAT+TGG | + | chr1.3:73229545-73229564 | MS.gene91284:intron | 20.0% |
| ! | AATTATCTGTTTATCATTGG+AGG | + | chr1.3:73229202-73229221 | MS.gene91284:intron | 25.0% |
| ! | AATTATGTAATCACATGTGT+AGG | - | chr1.3:73228966-73228985 | None:intergenic | 25.0% |
| ! | ATATAAAAACTGTAAGTCTG+CGG | - | chr1.3:73229273-73229292 | None:intergenic | 25.0% |
| ! | ATTATCTGTTTATCATTGGA+GGG | + | chr1.3:73229203-73229222 | MS.gene91284:intron | 25.0% |
| ! | TACTAACATCAAGATGATAA+TGG | + | chr1.3:73229598-73229617 | MS.gene91284:intron | 25.0% |
| !!! | ATGATAATGGTATGTGTTTT+AGG | + | chr1.3:73229611-73229630 | MS.gene91284:intron | 25.0% |
| ACATGAATTCTATATTGGAC+AGG | + | chr1.3:73229550-73229569 | MS.gene91284:intron | 30.0% | |
| ATTATGTGGTATGTGCTTAA+AGG | + | chr1.3:73229495-73229514 | MS.gene91284:intron | 30.0% | |
| CATGAATTCTATATTGGACA+GGG | + | chr1.3:73229551-73229570 | MS.gene91284:intron | 30.0% | |
| CGGAATTCAAATTCAAATTG+TGG | - | chr1.3:73229253-73229272 | None:intergenic | 30.0% | |
| TCTCATAAAGAATACCAAAG+TGG | + | chr1.3:73229469-73229488 | MS.gene91284:CDS | 30.0% | |
| TGATTACATAATTATCGGTG+TGG | + | chr1.3:73228973-73228992 | MS.gene91284:intron | 30.0% | |
| TTCTTTATGAGAGCAATAGT+TGG | - | chr1.3:73229461-73229480 | None:intergenic | 30.0% | |
| ! | GGACAAGCATTTAAGTATTT+TGG | + | chr1.3:73229349-73229368 | MS.gene91284:CDS | 30.0% |
| ! | TAAAGCTCAAGTCATCTTTT+TGG | + | chr1.3:73229698-73229717 | MS.gene91284:CDS | 30.0% |
| ! | TATTTTCTTTCGCTGAAATG+AGG | + | chr1.3:73229290-73229309 | MS.gene91284:intron | 30.0% |
| !! | ATAATGGTATGTGTTTTAGG+TGG | + | chr1.3:73229614-73229633 | MS.gene91284:intron | 30.0% |
| !! | TAATGGTATGTGTTTTAGGT+GGG | + | chr1.3:73229615-73229634 | MS.gene91284:intron | 30.0% |
| ATATTATTGGCAGTGCATAC+TGG | + | chr1.3:73229117-73229136 | MS.gene91284:intron | 35.0% | |
| CAATCTTTCTTCCAAAACCT+CGG | - | chr1.3:73229676-73229695 | None:intergenic | 35.0% | |
| GCAACAAATGAAGAAAATGG+CGG | + | chr1.3:73228703-73228722 | MS.gene91284:CDS | 35.0% | |
| GCTGCAACAAATGAAGAAAA+TGG | + | chr1.3:73228700-73228719 | MS.gene91284:CDS | 35.0% | |
| TACCAAAGTGGATGATTATG+TGG | + | chr1.3:73229481-73229500 | MS.gene91284:CDS | 35.0% | |
| TACCACATAATCATCCACTT+TGG | - | chr1.3:73229486-73229505 | None:intergenic | 35.0% | |
| TTGAGCTTCTCAGCTAAAAA+TGG | - | chr1.3:73229425-73229444 | None:intergenic | 35.0% | |
| ACGATCACTTGCTTTAACGA+CGG | - | chr1.3:73228801-73228820 | None:intergenic | 40.0% | |
| ATCTTGAAGCCCTAAGAGAA+AGG | + | chr1.3:73228675-73228694 | MS.gene91284:CDS | 40.0% | |
| GAGAAGAAGTCTTTCTCTGA+CGG | - | chr1.3:73228779-73228798 | None:intergenic | 40.0% | |
| GTAATCACATGTGTAGGTGT+CGG | - | chr1.3:73228960-73228979 | None:intergenic | 40.0% | |
| GTCATTTCTTCCGCGAAAAT+TGG | + | chr1.3:73228828-73228847 | MS.gene91284:CDS | 40.0% | |
| ! | AGCTCAAGTCATCTTTTTGG+AGG | + | chr1.3:73229701-73229720 | MS.gene91284:CDS | 40.0% |
| ! | GCTCAAGTCATCTTTTTGGA+GGG | + | chr1.3:73229702-73229721 | MS.gene91284:CDS | 40.0% |
| ! | TGCATTCAGTTTGCTTAGCT+TGG | + | chr1.3:73229054-73229073 | MS.gene91284:intron | 40.0% |
| !! | CGCAAACCAAAAGAAGTGTT+CGG | + | chr1.3:73229757-73229776 | MS.gene91284:CDS | 40.0% |
| !! | GATCGAGAATCAAGTACTGA+CGG | + | chr1.3:73228586-73228605 | MS.gene91284:CDS | 40.0% |
| !! | TAGGTGGGTTTTGATGAACT+TGG | + | chr1.3:73229630-73229649 | MS.gene91284:intron | 40.0% |
| !!! | CGAGGTTTTGGAAGAAAGAT+TGG | + | chr1.3:73229674-73229693 | MS.gene91284:CDS | 40.0% |
| AATCGTCAATACACCGTGCA+AGG | + | chr1.3:73229727-73229746 | MS.gene91284:CDS | 45.0% | |
| AGAAATTGTGCGAACCTTGC+AGG | - | chr1.3:73228855-73228874 | None:intergenic | 45.0% | |
| CACAGTTCATTCACAGGTTG+TGG | + | chr1.3:73229328-73229347 | MS.gene91284:intron | 45.0% | |
| CGAAGCAGCATATTGAGACT+CGG | + | chr1.3:73229372-73229391 | MS.gene91284:CDS | 45.0% | |
| CTTGATTCTCGATCACCTCT+TGG | - | chr1.3:73228580-73228599 | None:intergenic | 45.0% | |
| GAAATTGTGCGAACCTTGCA+GGG | - | chr1.3:73228854-73228873 | None:intergenic | 45.0% | |
| GGACAAAGGAAAGATCCAAG+AGG | + | chr1.3:73228562-73228581 | MS.gene91284:CDS | 45.0% | |
| GTCTTTCTCTGACGGAATCT+CGG | - | chr1.3:73228771-73228790 | None:intergenic | 45.0% | |
| TACTGATGACTTTAGCACCG+AGG | + | chr1.3:73229656-73229675 | MS.gene91284:CDS | 45.0% | |
| TGTGCTCACAGTTCATTCAC+AGG | + | chr1.3:73229322-73229341 | MS.gene91284:intron | 45.0% | |
| TTGAAGCCCTAAGAGAAAGG+AGG | + | chr1.3:73228678-73228697 | MS.gene91284:CDS | 45.0% | |
| TTGCAGCCTCCTTTCTCTTA+GGG | - | chr1.3:73228687-73228706 | None:intergenic | 45.0% | |
| !! | GTGGGTTTTGATGAACTTGG+TGG | + | chr1.3:73229633-73229652 | MS.gene91284:CDS | 45.0% |
| !! | TGACTTTAGCACCGAGGTTT+TGG | + | chr1.3:73229662-73229681 | MS.gene91284:CDS | 45.0% |
| AAGTGTTCGGCAGAGCACAA+CGG | + | chr1.3:73229770-73229789 | MS.gene91284:CDS | 50.0% | |
| CGGTGTGGATGTGTAGTGTT+AGG | + | chr1.3:73228988-73229007 | MS.gene91284:intron | 50.0% | |
| GTCATCGTCGAGACGTTCAA+GGG | - | chr1.3:73228654-73228673 | None:intergenic | 50.0% | |
| GTTGCAGCCTCCTTTCTCTT+AGG | - | chr1.3:73228688-73228707 | None:intergenic | 50.0% | |
| TCAATACACCGTGCAAGGTC+CGG | + | chr1.3:73229732-73229751 | MS.gene91284:CDS | 50.0% | |
| ! | GCTCTGCCGAACACTTCTTT+TGG | - | chr1.3:73229766-73229785 | None:intergenic | 50.0% |
| !! | CGAGAATCAAGTACTGACGG+TGG | + | chr1.3:73228589-73228608 | MS.gene91284:CDS | 50.0% |
| !!! | ACTTCTTTTGGTTTGCGCCC+CGG | - | chr1.3:73229754-73229773 | None:intergenic | 50.0% |
| AATACACCGTGCAAGGTCCG+GGG | + | chr1.3:73229734-73229753 | MS.gene91284:CDS | 55.0% | |
| CAATACACCGTGCAAGGTCC+GGG | + | chr1.3:73229733-73229752 | MS.gene91284:CDS | 55.0% | |
| CGTCATCGTCGAGACGTTCA+AGG | - | chr1.3:73228655-73228674 | None:intergenic | 55.0% | |
| GGTGTAATCTCCGTGTCCGA+GGG | - | chr1.3:73228750-73228769 | None:intergenic | 55.0% | |
| ! | CCTTGCAGGGCCAATTTTCG+CGG | - | chr1.3:73228841-73228860 | None:intergenic | 55.0% |
| CCGCGAAAATTGGCCCTGCA+AGG | + | chr1.3:73228838-73228857 | MS.gene91284:CDS | 60.0% | |
| CGGTGTAATCTCCGTGTCCG+AGG | - | chr1.3:73228751-73228770 | None:intergenic | 60.0% | |
| CGTAGCCGTTGGATCTCCCT+CGG | + | chr1.3:73228731-73228750 | MS.gene91284:CDS | 60.0% | |
| CGTGTCCGAGGGAGATCCAA+CGG | - | chr1.3:73228739-73228758 | None:intergenic | 60.0% | |
| CGTTGGATCTCCCTCGGACA+CGG | + | chr1.3:73228737-73228756 | MS.gene91284:CDS | 60.0% | |
| TTTGCGCCCCGGACCTTGCA+CGG | - | chr1.3:73229743-73229762 | None:intergenic | 65.0% | |
| ! | TGGCGGAGAAGCGTAGCCGT+TGG | + | chr1.3:73228720-73228739 | MS.gene91284:CDS | 65.0% |
| GACGGTGGCGCAAGCAGTCG+AGG | + | chr1.3:73228604-73228623 | MS.gene91284:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 73228560 | 73229815 | 73228560 | ID=MS.gene91284 |
| chr1.3 | mRNA | 73228560 | 73229815 | 73228560 | ID=MS.gene91284.t1;Parent=MS.gene91284 |
| chr1.3 | exon | 73228560 | 73228859 | 73228560 | ID=MS.gene91284.t1.exon1;Parent=MS.gene91284.t1 |
| chr1.3 | CDS | 73228560 | 73228859 | 73228560 | ID=cds.MS.gene91284.t1;Parent=MS.gene91284.t1 |
| chr1.3 | exon | 73229344 | 73229502 | 73229344 | ID=MS.gene91284.t1.exon2;Parent=MS.gene91284.t1 |
| chr1.3 | CDS | 73229344 | 73229502 | 73229344 | ID=cds.MS.gene91284.t1;Parent=MS.gene91284.t1 |
| chr1.3 | exon | 73229633 | 73229815 | 73229633 | ID=MS.gene91284.t1.exon3;Parent=MS.gene91284.t1 |
| chr1.3 | CDS | 73229633 | 73229815 | 73229633 | ID=cds.MS.gene91284.t1;Parent=MS.gene91284.t1 |
| Gene Sequence |
| Protein sequence |
