Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71889.t1 | XP_024638828.1 | 91.5 | 142 | 10 | 1 | 1 | 142 | 1 | 140 | 4.80E-61 | 243.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71889.t1 | G7JK81 | 93.6 | 110 | 7 | 0 | 1 | 110 | 1 | 110 | 3.7e-47 | 197.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054713 | MS.gene71889 | 0.8744 | 6.96E-68 | -1.69E-46 |
| MS.gene058042 | MS.gene71889 | 0.878227 | 3.33E-69 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71889.t1 | MTR_4g102040 | 93.636 | 110 | 7 | 0 | 1 | 110 | 1 | 110 | 4.62e-70 | 208 |
| MS.gene71889.t1 | MTR_5g011750 | 63.514 | 74 | 22 | 1 | 41 | 109 | 5 | 78 | 2.27e-24 | 90.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71889.t1 | AT2G27830 | 50.877 | 57 | 27 | 1 | 57 | 112 | 68 | 124 | 7.85e-11 | 57.8 |
Find 34 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGATCAGTGTTCTTCAAAAT+TGG | 0.195240 | 8.3:-19407892 | None:intergenic |
| GTTTCTCTGGCTGTGAATTA+TGG | 0.269497 | 8.3:-19407660 | None:intergenic |
| AAACTAATGACACTGTTCTT+AGG | 0.355610 | 8.3:+19407737 | MS.gene71889:CDS |
| AGACGAGAAGTTAGTGGGCT+TGG | 0.389634 | 8.3:+19408248 | MS.gene71889:CDS |
| CACCGTTGAAGATTTGATAA+AGG | 0.392539 | 8.3:+19407834 | MS.gene71889:CDS |
| GCTGTGAATTATGGAGTTTA+CGG | 0.413396 | 8.3:-19407651 | None:intergenic |
| AGAATTCAACGGGGTCTTAC+CGG | 0.420392 | 8.3:-19407626 | None:intergenic |
| GTTTACGGCAAGAATTCAAC+GGG | 0.423723 | 8.3:-19407636 | None:intergenic |
| ACATTGCCAGAGGGTTTCTC+TGG | 0.447363 | 8.3:-19407673 | None:intergenic |
| GTGACAATCGAAAACAGCTT+GGG | 0.499309 | 8.3:+19407781 | MS.gene71889:CDS |
| CTGCATGGTGCATGAAGAAA+CGG | 0.506540 | 8.3:-19408310 | None:intergenic |
| AAGCATGACTTCTCCGCCAC+AGG | 0.512058 | 8.3:+19407585 | None:intergenic |
| GAAGCGAGACGAGAAGTTAG+TGG | 0.521335 | 8.3:+19408242 | MS.gene71889:CDS |
| CTTCTCCGCCACAGGCTCAT+CGG | 0.524598 | 8.3:+19407593 | MS.gene71889:CDS |
| AGTTAGTGGGCTTGGAGTCA+AGG | 0.535890 | 8.3:+19408256 | MS.gene71889:CDS |
| GGTGACAATCGAAAACAGCT+TGG | 0.547560 | 8.3:+19407780 | MS.gene71889:CDS |
| TGCCACATTCTCATTCTGCA+TGG | 0.554134 | 8.3:-19408325 | None:intergenic |
| GTTTGCATCTTCAGCGTCAG+AGG | 0.563732 | 8.3:-19407718 | None:intergenic |
| TGCCTTTATCAAATCTTCAA+CGG | 0.571891 | 8.3:-19407836 | None:intergenic |
| AAGCGAGACGAGAAGTTAGT+GGG | 0.573862 | 8.3:+19408243 | MS.gene71889:CDS |
| AGATTTGATAAAGGCAGCGT+TGG | 0.574906 | 8.3:+19407843 | MS.gene71889:CDS |
| CCTCTGGCAATGTTGATCGT+CGG | 0.591836 | 8.3:+19407683 | MS.gene71889:CDS |
| TCACAGCCAGAGAAACCCTC+TGG | 0.594528 | 8.3:+19407667 | MS.gene71889:CDS |
| TTTCTCCGATGAGCCTGTGG+CGG | 0.602364 | 8.3:-19407598 | None:intergenic |
| GCCATCAAAACTGCTACTGA+AGG | 0.608756 | 8.3:+19407759 | MS.gene71889:CDS |
| TGCATGGTGCATGAAGAAAC+GGG | 0.610698 | 8.3:-19408309 | None:intergenic |
| AGTTTACGGCAAGAATTCAA+CGG | 0.621562 | 8.3:-19407637 | None:intergenic |
| ATTCAACGGGGTCTTACCGG+AGG | 0.655189 | 8.3:-19407623 | None:intergenic |
| CGACGATCAACATTGCCAGA+GGG | 0.655617 | 8.3:-19407682 | None:intergenic |
| TCAGATGTTGATGTTGTCTG+AGG | 0.666940 | 8.3:+19407810 | MS.gene71889:CDS |
| TTTATGAGAAGGATAAGAGG+AGG | 0.687109 | 8.3:+19407869 | MS.gene71889:CDS |
| CCGACGATCAACATTGCCAG+AGG | 0.706693 | 8.3:-19407683 | None:intergenic |
| ATGGTGCATGAAGAAACGGG+TGG | 0.830690 | 8.3:-19408306 | None:intergenic |
| TTTACGGCAAGAATTCAACG+GGG | 0.837986 | 8.3:-19407635 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCAAAATGATTGTTTTTTTT+AGG | - | chr8.3:19408208-19408227 | None:intergenic | 15.0% |
| !!! | AAAAAACAATCATTTTGAGT+TGG | + | chr8.3:19408210-19408229 | MS.gene71889:intron | 20.0% |
| ! | TTAGTAAGAAGTCATTACTT+TGG | - | chr8.3:19407954-19407973 | None:intergenic | 25.0% |
| !! | ATTACTTTGGAAAGTGAATT+GGG | - | chr8.3:19407941-19407960 | None:intergenic | 25.0% |
| !!! | AATGTTCTGTTTTTCATTCA+TGG | + | chr8.3:19408009-19408028 | MS.gene71889:intron | 25.0% |
| AAACTAATGACACTGTTCTT+AGG | + | chr8.3:19407737-19407756 | MS.gene71889:CDS | 30.0% | |
| AGCTCAAAACATAGAATTAG+GGG | - | chr8.3:19407984-19408003 | None:intergenic | 30.0% | |
| CAGCTCAAAACATAGAATTA+GGG | - | chr8.3:19407985-19408004 | None:intergenic | 30.0% | |
| TAATGAAGATCATAACACCT+TGG | - | chr8.3:19407916-19407935 | None:intergenic | 30.0% | |
| TCAGCTCAAAACATAGAATT+AGG | - | chr8.3:19407986-19408005 | None:intergenic | 30.0% | |
| TGCCTTTATCAAATCTTCAA+CGG | - | chr8.3:19407839-19407858 | None:intergenic | 30.0% | |
| ! | CATTACTTTGGAAAGTGAAT+TGG | - | chr8.3:19407942-19407961 | None:intergenic | 30.0% |
| ! | TGTTTTATGAGAAGGATAAG+AGG | + | chr8.3:19407866-19407885 | MS.gene71889:CDS | 30.0% |
| AGTTTACGGCAAGAATTCAA+CGG | - | chr8.3:19407640-19407659 | None:intergenic | 35.0% | |
| CACCGTTGAAGATTTGATAA+AGG | + | chr8.3:19407834-19407853 | MS.gene71889:CDS | 35.0% | |
| GCTGTGAATTATGGAGTTTA+CGG | - | chr8.3:19407654-19407673 | None:intergenic | 35.0% | |
| GGATCAGTGTTCTTCAAAAT+TGG | - | chr8.3:19407895-19407914 | None:intergenic | 35.0% | |
| TTTATGAGAAGGATAAGAGG+AGG | + | chr8.3:19407869-19407888 | MS.gene71889:CDS | 35.0% | |
| !! | TCTGTTTTTCATTCATGGAC+TGG | + | chr8.3:19408014-19408033 | MS.gene71889:intron | 35.0% |
| !! | TTTTGAAGAACACTGATCCA+AGG | + | chr8.3:19407896-19407915 | MS.gene71889:CDS | 35.0% |
| AGATTTGATAAAGGCAGCGT+TGG | + | chr8.3:19407843-19407862 | MS.gene71889:CDS | 40.0% | |
| GTGACAATCGAAAACAGCTT+GGG | + | chr8.3:19407781-19407800 | MS.gene71889:CDS | 40.0% | |
| GTTTACGGCAAGAATTCAAC+GGG | - | chr8.3:19407639-19407658 | None:intergenic | 40.0% | |
| GTTTCTCTGGCTGTGAATTA+TGG | - | chr8.3:19407663-19407682 | None:intergenic | 40.0% | |
| TCAGATGTTGATGTTGTCTG+AGG | + | chr8.3:19407810-19407829 | MS.gene71889:CDS | 40.0% | |
| TTTACGGCAAGAATTCAACG+GGG | - | chr8.3:19407638-19407657 | None:intergenic | 40.0% | |
| !! | ACCTTCAGTAGCAGTTTTGA+TGG | - | chr8.3:19407763-19407782 | None:intergenic | 40.0% |
| !! | CATTCATGGACTGGCTAATT+TGG | + | chr8.3:19408023-19408042 | MS.gene71889:intron | 40.0% |
| !!! | AGCGTTGGTGTTTTATGAGA+AGG | + | chr8.3:19407858-19407877 | MS.gene71889:CDS | 40.0% |
| !!! | CAATCATTTTGAGTTGGTGC+AGG | + | chr8.3:19408216-19408235 | MS.gene71889:intron | 40.0% |
| AAGCGAGACGAGAAGTTAGT+GGG | + | chr8.3:19408243-19408262 | MS.gene71889:CDS | 45.0% | |
| AGAATTCAACGGGGTCTTAC+CGG | - | chr8.3:19407629-19407648 | None:intergenic | 45.0% | |
| CTGCATGGTGCATGAAGAAA+CGG | - | chr8.3:19408313-19408332 | None:intergenic | 45.0% | |
| GCCATCAAAACTGCTACTGA+AGG | + | chr8.3:19407759-19407778 | MS.gene71889:CDS | 45.0% | |
| GGTGACAATCGAAAACAGCT+TGG | + | chr8.3:19407780-19407799 | MS.gene71889:CDS | 45.0% | |
| TGCATGGTGCATGAAGAAAC+GGG | - | chr8.3:19408312-19408331 | None:intergenic | 45.0% | |
| ACATTGCCAGAGGGTTTCTC+TGG | - | chr8.3:19407676-19407695 | None:intergenic | 50.0% | |
| ATGGTGCATGAAGAAACGGG+TGG | - | chr8.3:19408309-19408328 | None:intergenic | 50.0% | |
| CCTCTGGCAATGTTGATCGT+CGG | + | chr8.3:19407683-19407702 | MS.gene71889:CDS | 50.0% | |
| CGACGATCAACATTGCCAGA+GGG | - | chr8.3:19407685-19407704 | None:intergenic | 50.0% | |
| GAAGCGAGACGAGAAGTTAG+TGG | + | chr8.3:19408242-19408261 | MS.gene71889:CDS | 50.0% | |
| GCTCATCGGAGAAAAACCTC+CGG | + | chr8.3:19407607-19407626 | MS.gene71889:CDS | 50.0% | |
| GTTTGCATCTTCAGCGTCAG+AGG | - | chr8.3:19407721-19407740 | None:intergenic | 50.0% | |
| ! | GTTTTTCTCCGATGAGCCTG+TGG | - | chr8.3:19407604-19407623 | None:intergenic | 50.0% |
| !! | AGACGAGAAGTTAGTGGGCT+TGG | + | chr8.3:19408248-19408267 | MS.gene71889:CDS | 50.0% |
| !! | AGTTAGTGGGCTTGGAGTCA+AGG | + | chr8.3:19408256-19408275 | MS.gene71889:CDS | 50.0% |
| ATTCAACGGGGTCTTACCGG+AGG | - | chr8.3:19407626-19407645 | None:intergenic | 55.0% | |
| CCGACGATCAACATTGCCAG+AGG | - | chr8.3:19407686-19407705 | None:intergenic | 55.0% | |
| TCACAGCCAGAGAAACCCTC+TGG | + | chr8.3:19407667-19407686 | MS.gene71889:CDS | 55.0% | |
| TTTCTCCGATGAGCCTGTGG+CGG | - | chr8.3:19407601-19407620 | None:intergenic | 55.0% | |
| CTTCTCCGCCACAGGCTCAT+CGG | + | chr8.3:19407593-19407612 | MS.gene71889:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 19407589 | 19408337 | 19407589 | ID=MS.gene71889 |
| chr8.3 | mRNA | 19407589 | 19408337 | 19407589 | ID=MS.gene71889.t1;Parent=MS.gene71889 |
| chr8.3 | exon | 19407589 | 19407917 | 19407589 | ID=MS.gene71889.t1.exon1;Parent=MS.gene71889.t1 |
| chr8.3 | CDS | 19407589 | 19407917 | 19407589 | ID=cds.MS.gene71889.t1;Parent=MS.gene71889.t1 |
| chr8.3 | exon | 19408238 | 19408337 | 19408238 | ID=MS.gene71889.t1.exon2;Parent=MS.gene71889.t1 |
| chr8.3 | CDS | 19408238 | 19408337 | 19408238 | ID=cds.MS.gene71889.t1;Parent=MS.gene71889.t1 |
| Gene Sequence |
| Protein sequence |
