Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72294.t1 | AFK37815.1 | 96.2 | 78 | 3 | 0 | 2 | 79 | 135 | 212 | 3.90E-36 | 160.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72294.t1 | I3SC23 | 96.2 | 78 | 3 | 0 | 2 | 79 | 135 | 212 | 2.8e-36 | 160.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050710 | MS.gene72294 | 0.801749 | 7.77E-49 | -1.69E-46 |
| MS.gene05240 | MS.gene72294 | 0.806056 | 9.90E-50 | -1.69E-46 |
| MS.gene056688 | MS.gene72294 | 0.804671 | 1.93E-49 | -1.69E-46 |
| MS.gene056697 | MS.gene72294 | 0.805176 | 1.51E-49 | -1.69E-46 |
| MS.gene056920 | MS.gene72294 | 0.803257 | 3.80E-49 | -1.69E-46 |
| MS.gene058418 | MS.gene72294 | 0.801389 | 9.21E-49 | -1.69E-46 |
| MS.gene05926 | MS.gene72294 | 0.819932 | 8.96E-53 | -1.69E-46 |
| MS.gene060087 | MS.gene72294 | 0.837271 | 5.74E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72294.t1 | MTR_7g084690 | 94.872 | 78 | 4 | 0 | 2 | 79 | 135 | 212 | 9.02e-51 | 158 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTAAAATTGAATATGTATTC+AGG | 0.290425 | 7.1:+20459538 | None:intergenic |
| AATATGTATTCAGGAGTCTT+AGG | 0.315170 | 7.1:+20459547 | None:intergenic |
| CAAGGCCGTGTGCAATTGCC+TGG | 0.324039 | 7.1:-20459355 | MS.gene72294:CDS |
| TTAGAGATCCTCACAGAAAA+AGG | 0.343801 | 7.1:+20459475 | None:intergenic |
| ATCTCTAATTACTCTGCATT+GGG | 0.363990 | 7.1:-20459460 | MS.gene72294:CDS |
| GATCTCTAATTACTCTGCAT+TGG | 0.458386 | 7.1:-20459461 | MS.gene72294:CDS |
| GTCTAAGGGTGGTAAGGTTA+TGG | 0.486089 | 7.1:-20459572 | None:intergenic |
| AAATGTGTCTAAGGGTGGTA+AGG | 0.526644 | 7.1:-20459578 | None:intergenic |
| AAGGCCGTGTGCAATTGCCT+GGG | 0.544372 | 7.1:-20459354 | MS.gene72294:CDS |
| AGGCTGAGGATTTCATCAGA+AGG | 0.596136 | 7.1:-20459399 | MS.gene72294:CDS |
| TCAAGTTGATGATGAGGCTG+AGG | 0.596545 | 7.1:-20459413 | MS.gene72294:CDS |
| AAAACCCAGGCAATTGCACA+CGG | 0.600667 | 7.1:+20459350 | None:intergenic |
| TATGAGCAACTAAGGACGCA+AGG | 0.615711 | 7.1:-20459373 | MS.gene72294:CDS |
| CTCTAATTACTCTGCATTGG+GGG | 0.623371 | 7.1:-20459458 | MS.gene72294:CDS |
| TCTCTAATTACTCTGCATTG+GGG | 0.658768 | 7.1:-20459459 | MS.gene72294:CDS |
| AAATTGTCAAGTTGATGATG+AGG | 0.683682 | 7.1:-20459419 | MS.gene72294:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAAAATTGAATATGTATTC+AGG | + | chr7.1:20459378-20459397 | None:intergenic | 15.0% |
| AATATGTATTCAGGAGTCTT+AGG | + | chr7.1:20459369-20459388 | None:intergenic | 30.0% | |
| ATCTCTAATTACTCTGCATT+GGG | - | chr7.1:20459453-20459472 | MS.gene72294:CDS | 30.0% | |
| !! | AAATTGTCAAGTTGATGATG+AGG | - | chr7.1:20459494-20459513 | MS.gene72294:CDS | 30.0% |
| GATCTCTAATTACTCTGCAT+TGG | - | chr7.1:20459452-20459471 | MS.gene72294:CDS | 35.0% | |
| TCTCTAATTACTCTGCATTG+GGG | - | chr7.1:20459454-20459473 | MS.gene72294:CDS | 35.0% | |
| TTAGAGATCCTCACAGAAAA+AGG | + | chr7.1:20459441-20459460 | None:intergenic | 35.0% | |
| !! | GAAGGTTTTATGAGCAACTA+AGG | - | chr7.1:20459532-20459551 | MS.gene72294:CDS | 35.0% |
| ACAGAAAAAGGTGAGAGAAG+TGG | + | chr7.1:20459429-20459448 | None:intergenic | 40.0% | |
| CAGAAAAAGGTGAGAGAAGT+GGG | + | chr7.1:20459428-20459447 | None:intergenic | 40.0% | |
| CTCTAATTACTCTGCATTGG+GGG | - | chr7.1:20459455-20459474 | MS.gene72294:CDS | 40.0% | |
| TTGGGGGATAATGATGAGAT+TGG | - | chr7.1:20459471-20459490 | MS.gene72294:CDS | 40.0% | |
| ! | TTCTCTCACCTTTTTCTGTG+AGG | - | chr7.1:20459430-20459449 | MS.gene72294:CDS | 40.0% |
| AAAACCCAGGCAATTGCACA+CGG | + | chr7.1:20459566-20459585 | None:intergenic | 45.0% | |
| AGGCTGAGGATTTCATCAGA+AGG | - | chr7.1:20459514-20459533 | MS.gene72294:CDS | 45.0% | |
| TATGAGCAACTAAGGACGCA+AGG | - | chr7.1:20459540-20459559 | MS.gene72294:CDS | 45.0% | |
| ! | GTGCAATTGCCTGGGTTTTA+AGG | - | chr7.1:20459567-20459586 | MS.gene72294:CDS | 45.0% |
| !! | TCAAGTTGATGATGAGGCTG+AGG | - | chr7.1:20459500-20459519 | MS.gene72294:CDS | 45.0% |
| AAGGCCGTGTGCAATTGCCT+GGG | - | chr7.1:20459559-20459578 | MS.gene72294:CDS | 55.0% | |
| CAAGGCCGTGTGCAATTGCC+TGG | - | chr7.1:20459558-20459577 | MS.gene72294:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 20459348 | 20459587 | 20459348 | ID=MS.gene72294 |
| chr7.1 | mRNA | 20459348 | 20459587 | 20459348 | ID=MS.gene72294.t1;Parent=MS.gene72294 |
| chr7.1 | exon | 20459348 | 20459587 | 20459348 | ID=MS.gene72294.t1.exon1;Parent=MS.gene72294.t1 |
| chr7.1 | CDS | 20459348 | 20459587 | 20459348 | ID=cds.MS.gene72294.t1;Parent=MS.gene72294.t1 |
| Gene Sequence |
| Protein sequence |
