Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72369.t1 | XP_013457582.1 | 92.2 | 308 | 14 | 1 | 13 | 320 | 1 | 298 | 8.30E-162 | 579.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72369.t1 | Q8GXV5 | 66.9 | 308 | 92 | 1 | 13 | 320 | 1 | 298 | 2.1e-121 | 436.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72369.t1 | A0A072URB8 | 92.2 | 308 | 14 | 1 | 13 | 320 | 1 | 298 | 6.0e-162 | 579.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060512 | MS.gene72369 | -0.803596 | 3.23E-49 | -1.69E-46 |
| MS.gene060703 | MS.gene72369 | 0.800438 | 1.44E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72369.t1 | MTR_4g099290 | 92.208 | 308 | 14 | 1 | 13 | 320 | 1 | 298 | 0.0 | 575 |
| MS.gene72369.t1 | MTR_4g099290 | 91.045 | 268 | 14 | 1 | 13 | 280 | 1 | 258 | 2.17e-177 | 492 |
| MS.gene72369.t1 | MTR_4g099290 | 91.045 | 268 | 14 | 1 | 13 | 280 | 1 | 258 | 4.73e-177 | 490 |
| MS.gene72369.t1 | MTR_4g099290 | 88.263 | 213 | 15 | 1 | 13 | 225 | 1 | 203 | 5.00e-132 | 375 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72369.t1 | AT4G17050 | 66.883 | 308 | 92 | 1 | 13 | 320 | 1 | 298 | 4.95e-155 | 436 |
Find 90 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAATTGAATGTTCAAAATT+TGG | 0.124447 | 8.3:-21345425 | None:intergenic |
| TAGAGTGGAGTCATAAATTT+TGG | 0.203494 | 8.3:-21340602 | None:intergenic |
| TTCCTCCTTCTCCTCGTTTC+CGG | 0.204164 | 8.3:+21340395 | MS.gene72369:CDS |
| GGGCCATAAACATGACTTTC+TGG | 0.237286 | 8.3:-21343095 | None:intergenic |
| TCACCAGAAAGTCATGTTTA+TGG | 0.245916 | 8.3:+21343092 | MS.gene72369:CDS |
| ATAAAGTGTGAGCCCATTTC+AGG | 0.251326 | 8.3:-21343242 | None:intergenic |
| AATCAAAATCACAATTATAA+TGG | 0.260090 | 8.3:-21348262 | None:intergenic |
| CCAGCATGGTTTGTTGCTAT+TGG | 0.298210 | 8.3:+21346673 | MS.gene72369:CDS |
| AACTTATCACCTTCAGTATA+TGG | 0.299139 | 8.3:-21344241 | None:intergenic |
| ACAAACCATGCTGGTTATAA+TGG | 0.317445 | 8.3:-21346664 | None:intergenic |
| TGTCTTCAGGTACGCTGCAC+TGG | 0.322125 | 8.3:+21348192 | MS.gene72369:intron |
| TTCAGGCGGGTGATGTCATT+TGG | 0.330112 | 8.3:+21348000 | MS.gene72369:CDS |
| AAAGCTACTTCCAATGTCTT+TGG | 0.332406 | 8.3:+21346366 | MS.gene72369:CDS |
| AGGAGAAGGCATTTATCGTT+TGG | 0.348546 | 8.3:+21346697 | MS.gene72369:CDS |
| TGTGAATAGGTACCCGGTTC+AGG | 0.358704 | 8.3:+21347983 | MS.gene72369:intron |
| ATGACATCACCCGCCTGAAC+CGG | 0.360131 | 8.3:-21347996 | None:intergenic |
| AGGGGCTCTGGGACATGATT+TGG | 0.367925 | 8.3:-21345760 | None:intergenic |
| ATCAAAATCACAATTATAAT+GGG | 0.370700 | 8.3:-21348261 | None:intergenic |
| TGGAGAGTTTCTTAATGTTA+AGG | 0.373459 | 8.3:+21346546 | MS.gene72369:CDS |
| CCCTTACTTTGGAGATTAAG+AGG | 0.387626 | 8.3:-21340628 | None:intergenic |
| AGGGTTGTTTAGGTATTCAT+TGG | 0.390429 | 8.3:+21340510 | MS.gene72369:intron |
| GGAGAAGGCATTTATCGTTT+GGG | 0.390850 | 8.3:+21346698 | MS.gene72369:CDS |
| GTCTTCAGGTACGCTGCACT+GGG | 0.401122 | 8.3:+21348193 | MS.gene72369:intron |
| TTTGCAGAAAATTCAAGATC+AGG | 0.405975 | 8.3:+21343997 | MS.gene72369:intron |
| TTTATGGCCCATTGCCTGAT+TGG | 0.406017 | 8.3:+21343108 | MS.gene72369:CDS |
| GTCGAATTAACTAGGGGCTC+TGG | 0.407908 | 8.3:-21345772 | None:intergenic |
| GAGATTAAGAGGAGGTAGAG+TGG | 0.413997 | 8.3:-21340617 | None:intergenic |
| GTTTATCCGTCGAATTAACT+AGG | 0.436467 | 8.3:-21345780 | None:intergenic |
| TATTCATTGGCGCAAGAAGA+AGG | 0.459022 | 8.3:+21340523 | MS.gene72369:CDS |
| TCGAATTAACTAGGGGCTCT+GGG | 0.484257 | 8.3:-21345771 | None:intergenic |
| CATGGTTTGTTGCTATTGGA+AGG | 0.492316 | 8.3:+21346677 | MS.gene72369:CDS |
| TAGACACTGCGTGTGAATCC+AGG | 0.492364 | 8.3:-21343047 | None:intergenic |
| TAGGAATAATGTACCTCCCC+TGG | 0.496765 | 8.3:-21345820 | None:intergenic |
| TTTGGCAGGATCAATACATC+GGG | 0.498459 | 8.3:+21343203 | MS.gene72369:intron |
| AAAACCTCTCTACATCATGC+AGG | 0.502363 | 8.3:-21344026 | None:intergenic |
| TTGACGTAGCTTACCATTGT+GGG | 0.503289 | 8.3:-21348037 | None:intergenic |
| AGAGTTACCAATCAGGCAAT+GGG | 0.505467 | 8.3:-21343115 | None:intergenic |
| TCAAACCTTTCAGCTTTGCT+AGG | 0.509684 | 8.3:-21343271 | None:intergenic |
| TTTATCGTTTGGGTGATAGT+TGG | 0.509948 | 8.3:+21346708 | MS.gene72369:CDS |
| GGCGGGTGATGTCATTTGGA+TGG | 0.513354 | 8.3:+21348004 | MS.gene72369:CDS |
| ATTGAATGTTCAAAATTTGG+AGG | 0.514704 | 8.3:-21345422 | None:intergenic |
| CAGCCACTCGTTGAAACTCC+AGG | 0.517778 | 8.3:+21345802 | MS.gene72369:CDS |
| TTTATCCGTCGAATTAACTA+GGG | 0.520493 | 8.3:-21345779 | None:intergenic |
| TCAAATACCACAATAATAGC+AGG | 0.532548 | 8.3:-21345458 | None:intergenic |
| GTCAGAGTCACCAGTGCTCA+TGG | 0.534419 | 8.3:+21344212 | MS.gene72369:CDS |
| TAGTACCGGAAACGAGGAGA+AGG | 0.536548 | 8.3:-21340400 | None:intergenic |
| AATTCTTCGCTGAGCACAGA+AGG | 0.539539 | 8.3:-21340559 | None:intergenic |
| TGACATCACCCGCCTGAACC+GGG | 0.548155 | 8.3:-21347995 | None:intergenic |
| AAGAGTTACCAATCAGGCAA+TGG | 0.552009 | 8.3:-21343116 | None:intergenic |
| AGATTCATCTTTGTGCTCCA+CGG | 0.553805 | 8.3:+21344185 | MS.gene72369:intron |
| ATATTCAAGAGTTACCAATC+AGG | 0.554162 | 8.3:-21343122 | None:intergenic |
| ATTCATTGGCGCAAGAAGAA+GGG | 0.563652 | 8.3:+21340524 | MS.gene72369:CDS |
| ATGGCTCACACCATGAGCAC+TGG | 0.568857 | 8.3:-21344222 | None:intergenic |
| AAGTCATATGCCAAAGACAT+TGG | 0.570777 | 8.3:-21346376 | None:intergenic |
| TACCGGAAACGAGGAGAAGG+AGG | 0.570909 | 8.3:-21340397 | None:intergenic |
| AATAGGTACCCGGTTCAGGC+GGG | 0.576983 | 8.3:+21347987 | MS.gene72369:intron |
| GCTTACCATTGTGGGACGAA+TGG | 0.580025 | 8.3:-21348029 | None:intergenic |
| AAACCTCTCTACATCATGCA+GGG | 0.584130 | 8.3:-21344025 | None:intergenic |
| AAGAGATAGTACCGGAAACG+AGG | 0.584199 | 8.3:-21340406 | None:intergenic |
| CCAATAGCAACAAACCATGC+TGG | 0.587457 | 8.3:-21346673 | None:intergenic |
| TCCCCTGGAGTTTCAACGAG+TGG | 0.592547 | 8.3:-21345805 | None:intergenic |
| TTATTGTGGTATTTGAACGA+AGG | 0.594533 | 8.3:+21345465 | MS.gene72369:CDS |
| GAATAGGTACCCGGTTCAGG+CGG | 0.594571 | 8.3:+21347986 | MS.gene72369:intron |
| TTTGACGTAGCTTACCATTG+TGG | 0.595739 | 8.3:-21348038 | None:intergenic |
| TTAACATTAAGAAACTCTCC+AGG | 0.597029 | 8.3:-21346544 | None:intergenic |
| AGCCACTCGTTGAAACTCCA+GGG | 0.597354 | 8.3:+21345803 | MS.gene72369:CDS |
| CTTTGGCAGGATCAATACAT+CGG | 0.603198 | 8.3:+21343202 | MS.gene72369:intron |
| AGGTGGAGAAGAGATAGTAC+CGG | 0.604477 | 8.3:-21340414 | None:intergenic |
| TTGTTGCTATTGGAAGGAGA+AGG | 0.606426 | 8.3:+21346683 | MS.gene72369:CDS |
| TGACGCTCCTGCTATTATTG+TGG | 0.607609 | 8.3:+21345451 | MS.gene72369:CDS |
| TTCCCCTGCATGATGTAGAG+AGG | 0.611970 | 8.3:+21344022 | MS.gene72369:CDS |
| CACTGGGGAAAACTCCGACA+CGG | 0.622193 | 8.3:+21348209 | MS.gene72369:CDS |
| ATGTACCTAGCAAAGCTGAA+AGG | 0.629911 | 8.3:+21343266 | MS.gene72369:CDS |
| GAGGTCCATTATAACCAGCA+TGG | 0.636159 | 8.3:+21346659 | MS.gene72369:CDS |
| AGAGCCCCTAGTTAATTCGA+CGG | 0.642003 | 8.3:+21345774 | MS.gene72369:CDS |
| GCTTCGATTAGTAACGAAGG+TGG | 0.643859 | 8.3:-21340431 | None:intergenic |
| TGGCGCCATTCGTCCCACAA+TGG | 0.657597 | 8.3:+21348024 | MS.gene72369:CDS |
| CAGTGTCCACTCTCAAAATG+CGG | 0.668861 | 8.3:+21340366 | MS.gene72369:CDS |
| TGGTGTGAGCCATATACTGA+AGG | 0.678734 | 8.3:+21344232 | MS.gene72369:CDS |
| ACTCGTTGAAACTCCAGGGG+AGG | 0.679003 | 8.3:+21345807 | MS.gene72369:CDS |
| AACCTCTCTACATCATGCAG+GGG | 0.681979 | 8.3:-21344024 | None:intergenic |
| TTATCCGTCGAATTAACTAG+GGG | 0.689589 | 8.3:-21345778 | None:intergenic |
| TGGCAGGATCAATACATCGG+GGG | 0.695468 | 8.3:+21343205 | MS.gene72369:intron |
| ATAGCTTCGATTAGTAACGA+AGG | 0.696712 | 8.3:-21340434 | None:intergenic |
| TTACTTTGGAGATTAAGAGG+AGG | 0.697440 | 8.3:-21340625 | None:intergenic |
| GCCACTCGTTGAAACTCCAG+GGG | 0.709562 | 8.3:+21345804 | MS.gene72369:CDS |
| TTGTACAGAAGATACCGTGT+CGG | 0.721219 | 8.3:-21348223 | None:intergenic |
| TGGTGACTCTGACAACACCG+TGG | 0.731971 | 8.3:-21344202 | None:intergenic |
| TTGGCAGGATCAATACATCG+GGG | 0.751264 | 8.3:+21343204 | MS.gene72369:intron |
| TCTTCAGGTACGCTGCACTG+GGG | 0.784250 | 8.3:+21348194 | MS.gene72369:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 21340347 | 21348270 | 21340347 | ID=MS.gene72369 |
| chr8.3 | mRNA | 21340347 | 21348270 | 21340347 | ID=MS.gene72369.t1;Parent=MS.gene72369 |
| chr8.3 | exon | 21340347 | 21340456 | 21340347 | ID=MS.gene72369.t1.exon1;Parent=MS.gene72369.t1 |
| chr8.3 | CDS | 21340347 | 21340456 | 21340347 | ID=cds.MS.gene72369.t1;Parent=MS.gene72369.t1 |
| chr8.3 | exon | 21340522 | 21340643 | 21340522 | ID=MS.gene72369.t1.exon2;Parent=MS.gene72369.t1 |
| chr8.3 | CDS | 21340522 | 21340643 | 21340522 | ID=cds.MS.gene72369.t1;Parent=MS.gene72369.t1 |
| chr8.3 | exon | 21343042 | 21343129 | 21343042 | ID=MS.gene72369.t1.exon3;Parent=MS.gene72369.t1 |
| chr8.3 | CDS | 21343042 | 21343129 | 21343042 | ID=cds.MS.gene72369.t1;Parent=MS.gene72369.t1 |
| chr8.3 | exon | 21343211 | 21343287 | 21343211 | ID=MS.gene72369.t1.exon4;Parent=MS.gene72369.t1 |
| chr8.3 | CDS | 21343211 | 21343287 | 21343211 | ID=cds.MS.gene72369.t1;Parent=MS.gene72369.t1 |
| chr8.3 | exon | 21344004 | 21344043 | 21344004 | ID=MS.gene72369.t1.exon5;Parent=MS.gene72369.t1 |
| chr8.3 | CDS | 21344004 | 21344043 | 21344004 | ID=cds.MS.gene72369.t1;Parent=MS.gene72369.t1 |
| chr8.3 | exon | 21344187 | 21344253 | 21344187 | ID=MS.gene72369.t1.exon6;Parent=MS.gene72369.t1 |
| chr8.3 | CDS | 21344187 | 21344253 | 21344187 | ID=cds.MS.gene72369.t1;Parent=MS.gene72369.t1 |
| chr8.3 | exon | 21345401 | 21345486 | 21345401 | ID=MS.gene72369.t1.exon7;Parent=MS.gene72369.t1 |
| chr8.3 | CDS | 21345401 | 21345486 | 21345401 | ID=cds.MS.gene72369.t1;Parent=MS.gene72369.t1 |
| chr8.3 | exon | 21345747 | 21345828 | 21345747 | ID=MS.gene72369.t1.exon8;Parent=MS.gene72369.t1 |
| chr8.3 | CDS | 21345747 | 21345828 | 21345747 | ID=cds.MS.gene72369.t1;Parent=MS.gene72369.t1 |
| chr8.3 | exon | 21346352 | 21346408 | 21346352 | ID=MS.gene72369.t1.exon9;Parent=MS.gene72369.t1 |
| chr8.3 | CDS | 21346352 | 21346408 | 21346352 | ID=cds.MS.gene72369.t1;Parent=MS.gene72369.t1 |
| chr8.3 | exon | 21346529 | 21346567 | 21346529 | ID=MS.gene72369.t1.exon10;Parent=MS.gene72369.t1 |
| chr8.3 | CDS | 21346529 | 21346567 | 21346529 | ID=cds.MS.gene72369.t1;Parent=MS.gene72369.t1 |
| chr8.3 | exon | 21346659 | 21346729 | 21346659 | ID=MS.gene72369.t1.exon11;Parent=MS.gene72369.t1 |
| chr8.3 | CDS | 21346659 | 21346729 | 21346659 | ID=cds.MS.gene72369.t1;Parent=MS.gene72369.t1 |
| chr8.3 | exon | 21347992 | 21348045 | 21347992 | ID=MS.gene72369.t1.exon12;Parent=MS.gene72369.t1 |
| chr8.3 | CDS | 21347992 | 21348045 | 21347992 | ID=cds.MS.gene72369.t1;Parent=MS.gene72369.t1 |
| chr8.3 | exon | 21348201 | 21348270 | 21348201 | ID=MS.gene72369.t1.exon13;Parent=MS.gene72369.t1 |
| chr8.3 | CDS | 21348201 | 21348270 | 21348201 | ID=cds.MS.gene72369.t1;Parent=MS.gene72369.t1 |
| Gene Sequence |
| Protein sequence |
