AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene72412

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72412.t1 MTR_1g061170 94.130 460 24 2 1 458 1 459 0.0 876
MS.gene72412.t1 MTR_1g061170 94.318 352 19 1 107 458 14 364 0.0 682
MS.gene72412.t1 MTR_1g061170 93.640 283 18 0 176 458 1 283 0.0 550
MS.gene72412.t1 MTR_3g104550 41.011 356 182 9 33 384 32 363 1.29e-73 236
MS.gene72412.t1 MTR_5g029680 49.213 254 106 4 31 266 16 264 1.10e-72 238
MS.gene72412.t1 MTR_1g102860 51.082 231 105 5 25 248 15 244 2.86e-69 223
MS.gene72412.t1 MTR_7g095680 47.674 258 128 4 13 264 16 272 2.14e-68 222
MS.gene72412.t1 MTR_4g077970 41.118 304 151 7 45 324 10 309 2.22e-67 223
MS.gene72412.t1 MTR_1g071360 50.785 191 84 2 34 224 19 199 1.71e-57 193
MS.gene72412.t1 MTR_1g071360 50.785 191 84 2 34 224 16 196 3.63e-57 193
MS.gene72412.t1 MTR_2g100680 41.704 223 116 3 23 245 18 226 5.80e-49 174
MS.gene72412.t1 MTR_8g087540 44.199 181 98 2 43 221 8 187 9.34e-47 166
MS.gene72412.t1 MTR_6g043060 40.887 203 112 3 45 245 20 216 1.39e-46 164
MS.gene72412.t1 MTR_8g105780 37.868 272 151 6 45 308 20 281 9.36e-46 163
MS.gene72412.t1 MTR_8g083100 34.906 318 173 11 48 348 23 323 3.41e-45 164
MS.gene72412.t1 MTR_6g086805 40.704 199 115 2 45 243 9 204 7.43e-45 160
MS.gene72412.t1 MTR_2g100670 43.158 190 105 2 47 236 33 219 2.85e-44 157
MS.gene72412.t1 MTR_2g100670 43.158 190 105 2 47 236 141 327 1.90e-43 158
MS.gene72412.t1 MTR_3g101870 67.000 100 33 0 47 146 24 123 1.90e-40 149
MS.gene72412.t1 MTR_2g017890 37.113 194 111 2 45 234 13 199 2.45e-39 146
MS.gene72412.t1 MTR_7g091370 45.283 159 77 1 37 185 12 170 3.45e-39 141
MS.gene72412.t1 MTR_5g010680 62.245 98 37 0 41 138 18 115 3.53e-39 145
MS.gene72412.t1 MTR_5g017470 40.488 205 108 4 41 231 3 207 2.86e-38 140
MS.gene72412.t1 MTR_5g089170 62.626 99 37 0 41 139 19 117 3.83e-36 134
MS.gene72412.t1 MTR_0020s0020 61.957 92 35 0 47 138 33 124 1.89e-35 134
MS.gene72412.t1 MTR_1g100777 58.163 98 41 0 41 138 12 109 4.84e-35 132
MS.gene72412.t1 MTR_4g088900 43.750 160 74 6 36 185 27 180 2.85e-31 119
MS.gene72412.t1 MTR_4g045757 55.882 68 29 1 45 111 12 79 1.33e-18 80.5
MS.gene72412.t1 MTR_5g047190 44.565 92 44 3 55 139 1 92 2.60e-17 77.4
MS.gene72412.t1 MTR_4g077970 30.909 165 95 4 176 324 1 162 3.09e-13 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72412.t1 AT5G03720 58.687 259 98 4 27 279 35 290 8.01e-98 300
MS.gene72412.t1 AT5G03720 58.687 259 98 4 27 279 35 290 8.01e-98 300
MS.gene72412.t1 AT5G16820 39.791 382 187 9 45 406 25 383 1.17e-70 232
MS.gene72412.t1 AT5G16820 39.791 382 187 9 45 406 25 383 1.17e-70 232
MS.gene72412.t1 AT1G32330 46.008 263 120 4 26 266 11 273 4.46e-70 230
MS.gene72412.t1 AT1G32330 46.008 263 120 4 26 266 11 273 5.99e-70 230
MS.gene72412.t1 AT3G02990 47.368 266 122 5 42 294 18 278 3.37e-67 222
MS.gene72412.t1 AT4G17750 44.964 278 118 6 24 266 19 296 5.09e-66 220
MS.gene72412.t1 AT3G22830 46.753 231 114 2 23 244 37 267 3.39e-65 215
MS.gene72412.t1 AT2G26150 49.302 215 104 2 34 243 31 245 1.94e-64 211
MS.gene72412.t1 AT2G26150 49.302 215 104 2 34 243 31 245 1.94e-64 211
MS.gene72412.t1 AT3G63350 49.010 202 100 1 34 235 15 213 3.14e-61 201
MS.gene72412.t1 AT3G51910 47.170 212 93 3 37 243 19 216 6.76e-58 192
MS.gene72412.t1 AT5G43840 41.296 247 123 5 32 275 4 231 3.92e-56 188
MS.gene72412.t1 AT5G54070 40.090 222 121 4 41 258 65 278 2.08e-50 174
MS.gene72412.t1 AT1G67970 41.778 225 128 3 45 266 17 241 3.14e-50 175
MS.gene72412.t1 AT4G13980 39.899 198 116 1 48 245 24 218 6.31e-47 169
MS.gene72412.t1 AT3G24520 44.974 189 82 4 45 233 15 181 8.31e-45 159
MS.gene72412.t1 AT5G45710 39.896 193 115 1 43 234 9 201 8.01e-42 152
MS.gene72412.t1 AT5G45710 39.896 193 115 1 43 234 9 201 8.01e-42 152
MS.gene72412.t1 AT4G18880 43.850 187 103 1 43 227 11 197 2.35e-40 149
MS.gene72412.t1 AT5G62020 52.893 121 55 1 41 161 17 135 5.70e-38 140
MS.gene72412.t1 AT1G46264 65.263 95 33 0 47 141 34 128 5.86e-38 141
MS.gene72412.t1 AT4G11660 60.396 101 40 0 41 141 53 153 2.49e-37 140
MS.gene72412.t1 AT4G36990 36.792 212 126 3 35 238 2 213 2.50e-36 135
MS.gene72412.t1 AT2G41690 56.436 101 44 0 41 141 34 134 8.08e-36 133
MS.gene72412.t1 AT2G26150 45.775 142 72 2 107 243 49 190 1.65e-29 117
MS.gene72412.t1 AT2G26150 45.775 142 72 2 107 243 49 190 1.65e-29 117
MS.gene72412.t1 AT4G18870 45.161 93 50 1 42 134 8 99 6.29e-20 90.1
MS.gene72412.t1 AT4G18870 44.318 88 49 0 47 134 146 233 6.65e-19 87.4

Find 84 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAGATGAAGAAGAAAATT+TGG 0.205164 1.4:+50361583 None:intergenic
AATTCAGAAACTAATATTAT+TGG 0.230607 1.4:-50359750 MS.gene72412:CDS
TTTGAAGGGTTTCAATCTAT+TGG 0.240093 1.4:-50359639 MS.gene72412:CDS
GAAGTTTGGGTATTGATAAT+TGG 0.249663 1.4:-50359446 MS.gene72412:CDS
GAAACAAATGGTATCTTTCT+TGG 0.279705 1.4:-50360114 MS.gene72412:CDS
CTTTGTTGTTTGGGATCCTT+TGG 0.287745 1.4:-50361393 MS.gene72412:CDS
TCACCTTTAGTCATTTCTTT+AGG 0.294012 1.4:+50359846 None:intergenic
GATATGTGGGATATTGGTTT+TGG 0.304447 1.4:-50359468 MS.gene72412:CDS
TTATCAAGCAAGAAGATATA+TGG 0.310553 1.4:-50359605 MS.gene72412:CDS
TAGTGAGGTACCAACCAAAT+TGG 0.312029 1.4:-50359953 MS.gene72412:CDS
TGGTGTTAGCTTTGTTGTTT+GGG 0.316297 1.4:-50361402 MS.gene72412:CDS
TTGAAGGGTTTCAATCTATT+GGG 0.328172 1.4:-50359638 MS.gene72412:CDS
TCAGGAATAACTTCTTCATT+TGG 0.329440 1.4:+50359666 None:intergenic
GGAGAAGGATCATCTAGTTT+TGG 0.332158 1.4:-50359729 MS.gene72412:CDS
ATGGTGTTAGCTTTGTTGTT+TGG 0.339618 1.4:-50361403 MS.gene72412:CDS
ACAAACTTTCTTCTAACTCT+TGG 0.347851 1.4:+50360020 None:intergenic
AGTGAGGTACCAACCAAATT+GGG 0.367055 1.4:-50359952 MS.gene72412:CDS
TCTCTAATAGCAGGGAAATC+TGG 0.369457 1.4:-50360278 MS.gene72412:intron
ACAGCACCAACATTGAAGTT+AGG 0.369889 1.4:+50359579 None:intergenic
ATTAAGCTGACGAACAAAAC+TGG 0.370082 1.4:+50361322 None:intergenic
ATGTGGGATCCTATGAATTA+TGG 0.371954 1.4:-50359531 MS.gene72412:CDS
TTTGAAAGAAATGGTGGAAC+TGG 0.396168 1.4:+50361482 None:intergenic
AAAGATGAAGAAGAAAATTT+GGG 0.405694 1.4:+50361584 None:intergenic
AGGTTGAGATTGAAAGATTG+AGG 0.415211 1.4:-50360250 MS.gene72412:CDS
ATGTCAGATATGTGGGATAT+TGG 0.416242 1.4:-50359474 MS.gene72412:CDS
TGAGATTGAAAGATTGAGGA+AGG 0.438960 1.4:-50360246 MS.gene72412:CDS
TCAGATACTCCATAATTCAT+AGG 0.440777 1.4:+50359522 None:intergenic
GTTGGATCTTCTTCTGATGC+AGG 0.443789 1.4:-50360463 MS.gene72412:intron
TGAAACCCTTCAAAATTCTC+AGG 0.455295 1.4:+50359648 None:intergenic
GCAAGATTGACACCGACAAA+TGG 0.456806 1.4:-50360582 MS.gene72412:CDS
GAAGAAGAAAATGTCAAAGA+TGG 0.457561 1.4:-50359981 MS.gene72412:CDS
TAGTGATGTTCCTGTGCCTA+TGG 0.466887 1.4:-50361522 MS.gene72412:CDS
CTTTGAATCCGATCATATCA+TGG 0.467206 1.4:-50361433 MS.gene72412:CDS
GAATATGAAGCATCAGCTAT+AGG 0.469331 1.4:-50361557 MS.gene72412:CDS
TGACTATCTATTTCATCAAA+AGG 0.470522 1.4:+50359408 None:intergenic
TATCAAGCAAGAAGATATAT+GGG 0.482434 1.4:-50359604 MS.gene72412:CDS
CAGCAAGTTGGTAACTATGT+TGG 0.484482 1.4:-50360481 MS.gene72412:CDS
CAGCACCAACATTGAAGTTA+GGG 0.486019 1.4:+50359580 None:intergenic
AATTATGGAGTATCTGACTT+TGG 0.488166 1.4:-50359516 MS.gene72412:CDS
GAAACTAATATTATTGGAGA+AGG 0.489813 1.4:-50359744 MS.gene72412:CDS
CAAGATTGACACCGACAAAT+GGG 0.503413 1.4:-50360581 MS.gene72412:CDS
TCAGCTATAGGTGGTGGTAC+TGG 0.505325 1.4:-50361545 MS.gene72412:CDS
AGCGCGACTTAAGCAGAAGA+AGG 0.506475 1.4:-50360063 MS.gene72412:CDS
CTACCTAAAGAAATGACTAA+AGG 0.514970 1.4:-50359849 MS.gene72412:CDS
TCAAAAGGAGATTCATCAGC+TGG 0.517066 1.4:+50359423 None:intergenic
TTGAAAGATTGAGGAAGGAG+AGG 0.521106 1.4:-50360241 MS.gene72412:CDS
GATATGATCGGATTCAAAGA+AGG 0.522372 1.4:+50361437 None:intergenic
AGATCAAGTGAAAGATAGTG+AGG 0.532262 1.4:+50359870 None:intergenic
TTGAAAGAAATGGTGGAACT+GGG 0.536323 1.4:+50361483 None:intergenic
AACAACAAAGCTAACACCAT+TGG 0.540487 1.4:+50361406 None:intergenic
GTCCGGATTTGAAAACAACT+TGG 0.541092 1.4:+50360091 None:intergenic
TGAACAAAGACAGAAACAAA+TGG 0.541684 1.4:-50360126 MS.gene72412:CDS
CGAATGACGGCTAGTCACGC+AGG 0.554003 1.4:-50360176 MS.gene72412:CDS
ATTTGTCGGTGTCAATCTTG+CGG 0.568858 1.4:+50360584 None:intergenic
GATGAATATGCTGCAATGCA+TGG 0.570282 1.4:-50359786 MS.gene72412:CDS
TGGGACCCTAACTTCAATGT+TGG 0.572995 1.4:-50359585 MS.gene72412:CDS
ATAACTCTTGCAAACTCCAA+AGG 0.576307 1.4:+50361377 None:intergenic
GTGGTGACATGTCAGATATG+TGG 0.577350 1.4:-50359482 MS.gene72412:CDS
GAAGCATCAGCTATAGGTGG+TGG 0.582607 1.4:-50361551 MS.gene72412:CDS
ATCATATCATGGAGTTCCAA+TGG 0.583067 1.4:-50361422 MS.gene72412:CDS
TTGGAACTCCATGATATGAT+CGG 0.584421 1.4:+50361425 None:intergenic
ACTTGTTGAAAAGCATTCAA+AGG 0.586884 1.4:-50360522 MS.gene72412:CDS
CTCATCTGCAAACACTCCAT+AGG 0.589952 1.4:+50361506 None:intergenic
GTGAGGTACCAACCAAATTG+GGG 0.592193 1.4:-50359951 MS.gene72412:CDS
ATATGATCGGATTCAAAGAA+GGG 0.594477 1.4:+50361438 None:intergenic
TTGTTGTGTTTGAAGTGTCT+AGG 0.595204 1.4:+50361350 None:intergenic
TGCAAACACTCCATAGGCAC+AGG 0.597520 1.4:+50361512 None:intergenic
ACGCAGGTAATGTGAATCAA+AGG 0.601321 1.4:-50360160 MS.gene72412:CDS
TTTGATGAAATAGATAGTCA+AGG 0.610338 1.4:-50359405 MS.gene72412:CDS
TGGTGACATGTCAGATATGT+GGG 0.612349 1.4:-50359481 MS.gene72412:CDS
CAAACTGTGGGAATGAGATG+TGG 0.613012 1.4:-50359548 MS.gene72412:CDS
ACGTCTCAATCTCAGCAAGT+TGG 0.616416 1.4:-50360493 MS.gene72412:CDS
ACAGCAGCAACAACGAATGA+CGG 0.617265 1.4:-50360189 MS.gene72412:CDS
GACTTTGGAGTTGTTACAAG+TGG 0.618117 1.4:-50359501 MS.gene72412:CDS
AGCAGGGAAATCTGGAGTCG+AGG 0.619956 1.4:-50360270 MS.gene72412:CDS
TCAAAGATGGAAAGATAGTG+AGG 0.635058 1.4:-50359968 MS.gene72412:CDS
CTGTTGCTACTTCAAACTGT+GGG 0.638412 1.4:-50359560 MS.gene72412:CDS
GATGAAATAGATAGTCAAGG+TGG 0.640962 1.4:-50359402 MS.gene72412:CDS
GACTGTGTTAATGCAAGAAG+TGG 0.653059 1.4:-50360219 MS.gene72412:CDS
AAACTGTGGGAATGAGATGT+GGG 0.653938 1.4:-50359547 MS.gene72412:CDS
GCTGTTGCTACTTCAAACTG+TGG 0.663813 1.4:-50359561 MS.gene72412:CDS
TATGAAGCATCAGCTATAGG+TGG 0.681407 1.4:-50361554 MS.gene72412:CDS
CTTGCTGAGATTGAGACGTG+CGG 0.690178 1.4:+50360497 None:intergenic
TGAGGTACCAACCAAATTGG+GGG 0.710225 1.4:-50359950 MS.gene72412:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTCAGAAACTAATATTAT+TGG - chr1.4:50361241-50361260 MS.gene72412:intron 15.0%
!! TTACATTACATAGATTAATA+GGG + chr1.4:50359757-50359776 None:intergenic 15.0%
!!! TTTTCTTTTTTTTTTTGGTT+TGG - chr1.4:50359792-50359811 MS.gene72412:CDS 15.0%
!! AAAGATGAAGAAGAAAATTT+GGG + chr1.4:50359410-50359429 None:intergenic 20.0%
!! AACAATTTGATGTATCAATT+GGG - chr1.4:50359870-50359889 MS.gene72412:CDS 20.0%
!! GTTACATTACATAGATTAAT+AGG + chr1.4:50359758-50359777 None:intergenic 20.0%
!! TACATTACATAGATTAATAG+GGG + chr1.4:50359756-50359775 None:intergenic 20.0%
!! TCTTTCAAAAACATTTGATT+TGG - chr1.4:50359523-50359542 MS.gene72412:CDS 20.0%
!! TTATTCATTCTCAGATAATT+TGG - chr1.4:50360366-50360385 MS.gene72412:intron 20.0%
!!! AACATTTTTTCCTTTGAAAA+GGG + chr1.4:50361299-50361318 None:intergenic 20.0%
!!! TAACATTTTTTCCTTTGAAA+AGG + chr1.4:50361300-50361319 None:intergenic 20.0%
!!! TCAAATGTTTTTGAAAGAAA+TGG + chr1.4:50359521-50359540 None:intergenic 20.0%
! AGAATGAATAAACCAAACAT+AGG + chr1.4:50360357-50360376 None:intergenic 25.0%
! CAAAGATGAAGAAGAAAATT+TGG + chr1.4:50359411-50359430 None:intergenic 25.0%
! GAAACTAATATTATTGGAGA+AGG - chr1.4:50361247-50361266 MS.gene72412:intron 25.0%
! GAACAATTTGATGTATCAAT+TGG - chr1.4:50359869-50359888 MS.gene72412:CDS 25.0%
! TATCAAGCAAGAAGATATAT+GGG - chr1.4:50361387-50361406 MS.gene72412:CDS 25.0%
! TGACTATCTATTTCATCAAA+AGG + chr1.4:50361586-50361605 None:intergenic 25.0%
! TTATCAAGCAAGAAGATATA+TGG - chr1.4:50361386-50361405 MS.gene72412:CDS 25.0%
! TTCAATCAACAAACACAAAA+GGG + chr1.4:50359852-50359871 None:intergenic 25.0%
! TTTGATGAAATAGATAGTCA+AGG - chr1.4:50361586-50361605 MS.gene72412:CDS 25.0%
!! ATTTTCTTCTTCATCTTTGA+TGG - chr1.4:50359412-50359431 MS.gene72412:CDS 25.0%
!! TCACATCAAACCTTTTATAT+AGG - chr1.4:50360601-50360620 MS.gene72412:intron 25.0%
!! TGACATTTTCTTCTTCATTT+TGG + chr1.4:50361006-50361025 None:intergenic 25.0%
!! TTATTCCTGAGAATTTTGAA+GGG - chr1.4:50361338-50361357 MS.gene72412:CDS 25.0%
!!! AAGCTTGTCTTTTTTTGTTT+TGG - chr1.4:50360224-50360243 MS.gene72412:CDS 25.0%
!!! AATGTTTTTGAAAGAAATGG+TGG + chr1.4:50359518-50359537 None:intergenic 25.0%
!!! ACATTTTTTCCTTTGAAAAG+GGG + chr1.4:50361298-50361317 None:intergenic 25.0%
!!! AGTTTGTTAGCCTATATAAA+AGG + chr1.4:50360614-50360633 None:intergenic 25.0%
AAGATGAAAATCCCTTGAAA+AGG + chr1.4:50360122-50360141 None:intergenic 30.0%
ACAAACTTTCTTCTAACTCT+TGG + chr1.4:50360974-50360993 None:intergenic 30.0%
AGATGAAAATCCCTTGAAAA+GGG + chr1.4:50360121-50360140 None:intergenic 30.0%
ATATGATCGGATTCAAAGAA+GGG + chr1.4:50359556-50359575 None:intergenic 30.0%
ATTGAAAAACTCCCATTTGT+CGG + chr1.4:50360424-50360443 None:intergenic 30.0%
CTACCTAAAGAAATGACTAA+AGG - chr1.4:50361142-50361161 MS.gene72412:intron 30.0%
GAAACAAATGGTATCTTTCT+TGG - chr1.4:50360877-50360896 MS.gene72412:intron 30.0%
GAAGAAGAAAATGTCAAAGA+TGG - chr1.4:50361010-50361029 MS.gene72412:intron 30.0%
GTTCAATCAACAAACACAAA+AGG + chr1.4:50359853-50359872 None:intergenic 30.0%
TAACTGTATCTCTAATAGCA+GGG - chr1.4:50360705-50360724 MS.gene72412:intron 30.0%
TACAATACAGTTAGCTGTAT+TGG + chr1.4:50360267-50360286 None:intergenic 30.0%
TCAATCAACAAACACAAAAG+GGG + chr1.4:50359851-50359870 None:intergenic 30.0%
TCACCTTTAGTCATTTCTTT+AGG + chr1.4:50361148-50361167 None:intergenic 30.0%
TCAGATACTCCATAATTCAT+AGG + chr1.4:50361472-50361491 None:intergenic 30.0%
TCAGGAATAACTTCTTCATT+TGG + chr1.4:50361328-50361347 None:intergenic 30.0%
TGAACAAAGACAGAAACAAA+TGG - chr1.4:50360865-50360884 MS.gene72412:intron 30.0%
TTGAAGGGTTTCAATCTATT+GGG - chr1.4:50361353-50361372 MS.gene72412:CDS 30.0%
TTTGAAGGGTTTCAATCTAT+TGG - chr1.4:50361352-50361371 MS.gene72412:CDS 30.0%
! AATTATGGAGTATCTGACTT+TGG - chr1.4:50361475-50361494 MS.gene72412:CDS 30.0%
! GTTATTCCTGAGAATTTTGA+AGG - chr1.4:50361337-50361356 MS.gene72412:CDS 30.0%
! TTCAATGAAGGTTTTCAGAA+AGG - chr1.4:50360438-50360457 MS.gene72412:intron 30.0%
!! AAATGGGAGTTTTTCAATGA+AGG - chr1.4:50360426-50360445 MS.gene72412:intron 30.0%
!! ACTTGTTGAAAAGCATTCAA+AGG - chr1.4:50360469-50360488 MS.gene72412:CDS 30.0%
!! GAAGTTTGGGTATTGATAAT+TGG - chr1.4:50361545-50361564 MS.gene72412:CDS 30.0%
!! TGTGTTTTTGTTACTACTAG+TGG - chr1.4:50359964-50359983 MS.gene72412:CDS 30.0%
!!! GATATTGGTTTTGGAAGTTT+GGG - chr1.4:50361532-50361551 MS.gene72412:CDS 30.0%
!!! GGAGAGTTTTTTTCATCATT+TGG + chr1.4:50359389-50359408 None:intergenic 30.0%
AACAACAAAGCTAACACCAT+TGG + chr1.4:50359588-50359607 None:intergenic 35.0%
ACTACACAATGCAATCAATC+AGG + chr1.4:50360040-50360059 None:intergenic 35.0%
AGATCAAGTGAAAGATAGTG+AGG + chr1.4:50361124-50361143 None:intergenic 35.0%
AGGTTGAGATTGAAAGATTG+AGG - chr1.4:50360741-50360760 MS.gene72412:intron 35.0%
ATAACTCTTGCAAACTCCAA+AGG + chr1.4:50359617-50359636 None:intergenic 35.0%
ATCATATCATGGAGTTCCAA+TGG - chr1.4:50359569-50359588 MS.gene72412:CDS 35.0%
ATGTCAGATATGTGGGATAT+TGG - chr1.4:50361517-50361536 MS.gene72412:CDS 35.0%
ATGTCTATATTCCCCCAATT+TGG + chr1.4:50361055-50361074 None:intergenic 35.0%
ATGTGGGATCCTATGAATTA+TGG - chr1.4:50361460-50361479 MS.gene72412:CDS 35.0%
ATTAAGCTGACGAACAAAAC+TGG + chr1.4:50359672-50359691 None:intergenic 35.0%
CAATCAACAAACACAAAAGG+GGG + chr1.4:50359850-50359869 None:intergenic 35.0%
CGTCAGCTTAATACTTATGT+GGG - chr1.4:50359680-50359699 MS.gene72412:CDS 35.0%
CTAACTGTATCTCTAATAGC+AGG - chr1.4:50360704-50360723 MS.gene72412:intron 35.0%
CTTTGAATCCGATCATATCA+TGG - chr1.4:50359558-50359577 MS.gene72412:CDS 35.0%
GATATGATCGGATTCAAAGA+AGG + chr1.4:50359557-50359576 None:intergenic 35.0%
GATGAAATAGATAGTCAAGG+TGG - chr1.4:50361589-50361608 MS.gene72412:CDS 35.0%
TCAAAGATGGAAAGATAGTG+AGG - chr1.4:50361023-50361042 MS.gene72412:intron 35.0%
TCGTCAGCTTAATACTTATG+TGG - chr1.4:50359679-50359698 MS.gene72412:CDS 35.0%
TGAAACCCTTCAAAATTCTC+AGG + chr1.4:50361346-50361365 None:intergenic 35.0%
TGAGATTGAAAGATTGAGGA+AGG - chr1.4:50360745-50360764 MS.gene72412:intron 35.0%
TTGGAACTCCATGATATGAT+CGG + chr1.4:50359569-50359588 None:intergenic 35.0%
TTTCTTGAGTCTCCTATGTT+TGG - chr1.4:50360342-50360361 MS.gene72412:intron 35.0%
! ACTTTTCACAATGCCTCTAA+TGG - chr1.4:50359730-50359749 MS.gene72412:CDS 35.0%
! ATAGTGAGGAGAGTTTTCAA+TGG + chr1.4:50361110-50361129 None:intergenic 35.0%
! GAATATGAAGCATCAGCTAT+AGG - chr1.4:50359434-50359453 MS.gene72412:CDS 35.0%
! TTGAAAGAAATGGTGGAACT+GGG + chr1.4:50359511-50359530 None:intergenic 35.0%
! TTGTTGTGTTTGAAGTGTCT+AGG + chr1.4:50359644-50359663 None:intergenic 35.0%
! TTTGAAAGAAATGGTGGAAC+TGG + chr1.4:50359512-50359531 None:intergenic 35.0%
!! ATAATTTGGTGTTTGAGCAC+AGG - chr1.4:50360380-50360399 MS.gene72412:intron 35.0%
!! ATGGTGTTAGCTTTGTTGTT+TGG - chr1.4:50359588-50359607 MS.gene72412:CDS 35.0%
!! TAATTTGGTGTTTGAGCACA+GGG - chr1.4:50360381-50360400 MS.gene72412:intron 35.0%
!! TGGTGTTAGCTTTGTTGTTT+GGG - chr1.4:50359589-50359608 MS.gene72412:CDS 35.0%
!!! GATATGTGGGATATTGGTTT+TGG - chr1.4:50361523-50361542 MS.gene72412:CDS 35.0%
!!! GGATATTGGTTTTGGAAGTT+TGG - chr1.4:50361531-50361550 MS.gene72412:CDS 35.0%
AAACTGTGGGAATGAGATGT+GGG - chr1.4:50361444-50361463 MS.gene72412:CDS 40.0%
ACACAATGCAATCAATCAGG+AGG + chr1.4:50360037-50360056 None:intergenic 40.0%
ACAGCACCAACATTGAAGTT+AGG + chr1.4:50361415-50361434 None:intergenic 40.0%
ACGCAGGTAATGTGAATCAA+AGG - chr1.4:50360831-50360850 MS.gene72412:intron 40.0%
AGATTAATAGGGGCCATTAG+AGG + chr1.4:50359746-50359765 None:intergenic 40.0%
CAAGATTGACACCGACAAAT+GGG - chr1.4:50360410-50360429 MS.gene72412:intron 40.0%
CAGCACCAACATTGAAGTTA+GGG + chr1.4:50361414-50361433 None:intergenic 40.0%
CTATATTCCCCCAATTTGGT+TGG + chr1.4:50361051-50361070 None:intergenic 40.0%
GACTGTGTTAATGCAAGAAG+TGG - chr1.4:50360772-50360791 MS.gene72412:intron 40.0%
GATGAATATGCTGCAATGCA+TGG - chr1.4:50361205-50361224 MS.gene72412:intron 40.0%
GTCCGGATTTGAAAACAACT+TGG + chr1.4:50360903-50360922 None:intergenic 40.0%
TCAAAAGGAGATTCATCAGC+TGG + chr1.4:50361571-50361590 None:intergenic 40.0%
TCTCTAATAGCAGGGAAATC+TGG - chr1.4:50360713-50360732 MS.gene72412:intron 40.0%
TGAAACACAGAATCCTACCT+TGG + chr1.4:50360145-50360164 None:intergenic 40.0%
TGGTGACATGTCAGATATGT+GGG - chr1.4:50361510-50361529 MS.gene72412:CDS 40.0%
TTAAGTCGCGCTAAAAAGTC+CGG + chr1.4:50360920-50360939 None:intergenic 40.0%
TTGAAAGATTGAGGAAGGAG+AGG - chr1.4:50360750-50360769 MS.gene72412:intron 40.0%
! AATGTTGCTCTCCCTTTTCA+AGG - chr1.4:50360107-50360126 MS.gene72412:CDS 40.0%
! AGTGAGGTACCAACCAAATT+GGG - chr1.4:50361039-50361058 MS.gene72412:intron 40.0%
! ATGTTGCTCTCCCTTTTCAA+GGG - chr1.4:50360108-50360127 MS.gene72412:CDS 40.0%
! CAAGGGATTTTCATCTTCCA+AGG - chr1.4:50360125-50360144 MS.gene72412:CDS 40.0%
! CTGTTGCTACTTCAAACTGT+GGG - chr1.4:50361431-50361450 MS.gene72412:CDS 40.0%
! CTTGATGAACCCCTTTTCAA+AGG - chr1.4:50361286-50361305 MS.gene72412:intron 40.0%
! GGAGAGTTTTCAATGGAAAC+TGG + chr1.4:50361103-50361122 None:intergenic 40.0%
! GGATTTTCATCTTCCAAGGT+AGG - chr1.4:50360129-50360148 MS.gene72412:CDS 40.0%
! TAGTGAGGTACCAACCAAAT+TGG - chr1.4:50361038-50361057 MS.gene72412:intron 40.0%
! TATGAAGCATCAGCTATAGG+TGG - chr1.4:50359437-50359456 MS.gene72412:CDS 40.0%
!! ATTTGTCGGTGTCAATCTTG+CGG + chr1.4:50360410-50360429 None:intergenic 40.0%
!! CAGCAAGTTGGTAACTATGT+TGG - chr1.4:50360510-50360529 MS.gene72412:CDS 40.0%
!! CTTTGTTGTTTGGGATCCTT+TGG - chr1.4:50359598-50359617 MS.gene72412:CDS 40.0%
!! GACTTTGGAGTTGTTACAAG+TGG - chr1.4:50361490-50361509 MS.gene72412:CDS 40.0%
!! GGAGAAGGATCATCTAGTTT+TGG - chr1.4:50361262-50361281 MS.gene72412:intron 40.0%
!! GGCCAAGTTGTTTTCAAATC+CGG - chr1.4:50360898-50360917 MS.gene72412:intron 40.0%
AACAAACACAAAAGGGGGAG+AGG + chr1.4:50359845-50359864 None:intergenic 45.0%
ACAAACACAAAAGGGGGAGA+GGG + chr1.4:50359844-50359863 None:intergenic 45.0%
ACAGCAGCAACAACGAATGA+CGG - chr1.4:50360802-50360821 MS.gene72412:intron 45.0%
ACGTCTCAATCTCAGCAAGT+TGG - chr1.4:50360498-50360517 MS.gene72412:CDS 45.0%
ACTGCTGGAATTGGCAACAT+AGG - chr1.4:50360080-50360099 MS.gene72412:CDS 45.0%
ATGTTGCCAATTCCAGCAGT+AGG + chr1.4:50360080-50360099 None:intergenic 45.0%
CAAACTGTGGGAATGAGATG+TGG - chr1.4:50361443-50361462 MS.gene72412:CDS 45.0%
CTCATCTGCAAACACTCCAT+AGG + chr1.4:50359488-50359507 None:intergenic 45.0%
GCAAGATTGACACCGACAAA+TGG - chr1.4:50360409-50360428 MS.gene72412:intron 45.0%
GTGGTGACATGTCAGATATG+TGG - chr1.4:50361509-50361528 MS.gene72412:CDS 45.0%
GTTGGATCTTCTTCTGATGC+AGG - chr1.4:50360528-50360547 MS.gene72412:CDS 45.0%
TAGTGATGTTCCTGTGCCTA+TGG - chr1.4:50359469-50359488 MS.gene72412:CDS 45.0%
TGGGACCCTAACTTCAATGT+TGG - chr1.4:50361406-50361425 MS.gene72412:CDS 45.0%
! GCTGTTGCTACTTCAAACTG+TGG - chr1.4:50361430-50361449 MS.gene72412:CDS 45.0%
! GTGAGGTACCAACCAAATTG+GGG - chr1.4:50361040-50361059 MS.gene72412:intron 45.0%
! TGAGGTACCAACCAAATTGG+GGG - chr1.4:50361041-50361060 MS.gene72412:intron 45.0%
!!! TTTTTTTTTCTTTTTTTTTT+TGG - chr1.4:50359787-50359806 MS.gene72412:CDS 5.0%
AGCGCGACTTAAGCAGAAGA+AGG - chr1.4:50360928-50360947 MS.gene72412:intron 50.0%
CATTCAGAGACTCCTACTGC+TGG - chr1.4:50360065-50360084 MS.gene72412:CDS 50.0%
CTTGCTGAGATTGAGACGTG+CGG + chr1.4:50360497-50360516 None:intergenic 50.0%
GAGACTCCTACTGCTGGAAT+TGG - chr1.4:50360071-50360090 MS.gene72412:CDS 50.0%
TGCAAACACTCCATAGGCAC+AGG + chr1.4:50359482-50359501 None:intergenic 50.0%
! GAAGCATCAGCTATAGGTGG+TGG - chr1.4:50359440-50359459 MS.gene72412:CDS 50.0%
! TCAGCTATAGGTGGTGGTAC+TGG - chr1.4:50359446-50359465 MS.gene72412:CDS 50.0%
! AGCAGGGAAATCTGGAGTCG+AGG - chr1.4:50360721-50360740 MS.gene72412:intron 55.0%
! CGAATGACGGCTAGTCACGC+AGG - chr1.4:50360815-50360834 MS.gene72412:intron 60.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 50359380 50361633 50359380 ID=MS.gene72412
chr1.4 mRNA 50359380 50361633 50359380 ID=MS.gene72412.t1;Parent=MS.gene72412
chr1.4 exon 50361316 50361633 50361316 ID=MS.gene72412.t1.exon1;Parent=MS.gene72412.t1
chr1.4 CDS 50361316 50361633 50361316 ID=cds.MS.gene72412.t1;Parent=MS.gene72412.t1
chr1.4 exon 50360464 50360611 50360464 ID=MS.gene72412.t1.exon2;Parent=MS.gene72412.t1
chr1.4 CDS 50360464 50360611 50360464 ID=cds.MS.gene72412.t1;Parent=MS.gene72412.t1
chr1.4 exon 50359380 50360290 50359380 ID=MS.gene72412.t1.exon3;Parent=MS.gene72412.t1
chr1.4 CDS 50359380 50360290 50359380 ID=cds.MS.gene72412.t1;Parent=MS.gene72412.t1
Gene Sequence

>MS.gene72412

ATGAATCCAAATGATGAAAAAAACTCTCCCAAATTTTCTTCTTCATCTTTGATGGAATATGAAGCATCAGCTATAGGTGGTGGTACTGGTAGTGATGTTCCTGTGCCTATGGAGTGTTTGCAGATGAGCCCAGTTCCACCATTTCTTTCAAAAACATTTGATTTGGTTGATGACCCTTCTTTGAATCCGATCATATCATGGAGTTCCAATGGTGTTAGCTTTGTTGTTTGGGATCCTTTGGAGTTTGCAAGAGTTATCTTGCCTAGACACTTCAAACACAACAATTTCTCCAGTTTTGTTCGTCAGCTTAATACTTATGTGGGTATTTTTGCAATTTATATTGTTTTGCTACTTTTCACAATGCCTCTAATGGCCCCTATTAATCTATGTAATGTAACTAACTATTTTTTTTTTTTCTTTTTTTTTTTGGTTTGGAAGTATTTGTAACTAACTATTTAATTCCCTCTCCCCCTTTTGTGTTTGTTGATTGAACAATTTGATGTATCAATTGGGAATGTAAATAATTTATTTGCTGATTGTATTGTATGTAAAACTTGCATATTTTGCTATATGCTCTTTGTAATTGTGTTTTTGTTACTACTAGTGGAACTATGTAATAAAATAAGCACACACACATTGCAGATATGTTTGTTTCCTCCTGATTGATTGCATTGTGTAGTTATAGCATTCAGAGACTCCTACTGCTGGAATTGGCAACATAGGAGACAATGTTGCTCTCCCTTTTCAAGGGATTTTCATCTTCCAAGGTAGGATTCTGTGTTTCAATGATGTGTTTTGTGTGTTCTTTGACTTTAGATTATATTTTTGTGATGCATTTTATCATAAGCTTGTCTTTTTTTGTTTTGGATTACAGATTGCATTCTCCAATACAGCTAACTGTATTGTATCATGTAACTATTTTGTTTTTGCTTTGATATATGTTACTTGTATTTTTTTCTTTCTTTCTTGAGTCTCCTATGTTTGGTTTATTCATTCTCAGATAATTTGGTGTTTGAGCACAGGGATTCCGCAAGATTGACACCGACAAATGGGAGTTTTTCAATGAAGGTTTTCAGAAAGGTAAGAAGCACTTGTTGAAAAGCATTCAAAGGCGCCGCACGTCTCAATCTCAGCAAGTTGGTAACTATGTTGGATCTTCTTCTGATGCAGGTATTAGAAAAATATAAATAGTATTAATATGAGAGAAGTGATTATAAGTGTTCACATCAAACCTTTTATATAGGCTAACAAACTAGTAACTAACTAACTAATTATATTTCTATATATGCTTACAAACTAGTTACAAACTTCTACTAGTAACTAACTAACTGTATCTCTAATAGCAGGGAAATCTGGAGTCGAGGTTGAGATTGAAAGATTGAGGAAGGAGAGGACTGTGTTAATGCAAGAAGTGGTTGATTTACAGCAGCAACAACGAATGACGGCTAGTCACGCAGGTAATGTGAATCAAAGGCTTCAATCTGCTGAACAAAGACAGAAACAAATGGTATCTTTCTTGGCCAAGTTGTTTTCAAATCCGGACTTTTTAGCGCGACTTAAGCAGAAGAAGGAACAGAAAGATATAGAATCTCCAAGAGTTAGAAGAAAGTTTGTTAAGCAAAACCAAAATGAAGAAGAAAATGTCAAAGATGGAAAGATAGTGAGGTACCAACCAAATTGGGGGAATATAGACATGTCTTGTGAAACTCAAGAACTAAGTCCAGTTTCCATTGAAAACTCTCCTCACTATCTTTCACTTGATCTACCTAAAGAAATGACTAAAGGTGAAGAAGATTTTACTTCTCAAATTGAGAATATTTCATCAGATGAATATGCTGCAATGCATGGAATTTTCATGTCAAATTCAGAAACTAATATTATTGGAGAAGGATCATCTAGTTTTGGACTTGATGAACCCCTTTTCAAAGGAAAAAATGTTATTAGTCCAAATGAAGAAGTTATTCCTGAGAATTTTGAAGGGTTTCAATCTATTGGGACTGAAAGTATTATCAAGCAAGAAGATATATGGGACCCTAACTTCAATGTTGGTGCTGTTGCTACTTCAAACTGTGGGAATGAGATGTGGGATCCTATGAATTATGGAGTATCTGACTTTGGAGTTGTTACAAGTGGTGACATGTCAGATATGTGGGATATTGGTTTTGGAAGTTTGGGTATTGATAATTGGCCAGCTGATGAATCTCCTTTTGATGAAATAGATAGTCAAGGTGGTAAGCATGATAGACCTACCTAA
Protein sequence

>MS.gene72412.t1

MNPNDEKNSPKFSSSSLMEYEASAIGGGTGSDVPVPMECLQMSPVPPFLSKTFDLVDDPSLNPIISWSSNGVSFVVWDPLEFARVILPRHFKHNNFSSFVRQLNTYGFRKIDTDKWEFFNEGFQKGKKHLLKSIQRRRTSQSQQVGNYVGSSSDAAGKSGVEVEIERLRKERTVLMQEVVDLQQQQRMTASHAGNVNQRLQSAEQRQKQMVSFLAKLFSNPDFLARLKQKKEQKDIESPRVRRKFVKQNQNEEENVKDGKIVRYQPNWGNIDMSCETQELSPVSIENSPHYLSLDLPKEMTKGEEDFTSQIENISSDEYAAMHGIFMSNSETNIIGEGSSSFGLDEPLFKGKNVISPNEEVIPENFEGFQSIGTESIIKQEDIWDPNFNVGAVATSNCGNEMWDPMNYGVSDFGVVTSGDMSDMWDIGFGSLGIDNWPADESPFDEIDSQGGKHDRPT