Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054412.t1 | XP_003626177.1 | 97.6 | 164 | 4 | 0 | 1 | 164 | 1 | 164 | 3.90E-83 | 317.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054412.t1 | Q8WWF6 | 57.6 | 66 | 25 | 1 | 17 | 82 | 4 | 66 | 2.1e-13 | 77.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054412.t1 | G7KWI3 | 97.6 | 164 | 4 | 0 | 1 | 164 | 1 | 164 | 2.8e-83 | 317.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050743 | MS.gene054412 | 0.887697 | 1.14E-72 | -1.69E-46 |
| MS.gene050946 | MS.gene054412 | 0.835485 | 1.63E-56 | -1.69E-46 |
| MS.gene054412 | MS.gene057249 | 0.831867 | 1.31E-55 | -1.69E-46 |
| MS.gene054412 | MS.gene06818 | 0.843386 | 1.45E-58 | -1.69E-46 |
| MS.gene054412 | MS.gene072546 | 0.955433 | 3.07E-113 | -1.69E-46 |
| MS.gene054412 | MS.gene073800 | 0.814996 | 1.16E-51 | -1.69E-46 |
| MS.gene054412 | MS.gene30248 | 0.806676 | 7.33E-50 | -1.69E-46 |
| MS.gene054412 | MS.gene30890 | 0.824201 | 9.18E-54 | -1.69E-46 |
| MS.gene054412 | MS.gene47348 | 0.803598 | 3.23E-49 | -1.69E-46 |
| MS.gene054412 | MS.gene62679 | 0.801269 | 9.75E-49 | -1.69E-46 |
| MS.gene054412 | MS.gene72412 | 0.812349 | 4.43E-51 | -1.69E-46 |
| MS.gene054412 | MS.gene94484 | 0.813728 | 2.21E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene038416 | MS.gene054412 | PPI |
| MS.gene062293 | MS.gene054412 | PPI |
| MS.gene017181 | MS.gene054412 | PPI |
| MS.gene065503 | MS.gene054412 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054412.t1 | MTR_7g112360 | 97.561 | 164 | 4 | 0 | 1 | 164 | 1 | 164 | 3.52e-119 | 333 |
| MS.gene054412.t1 | MTR_7g112360 | 97.872 | 94 | 2 | 0 | 1 | 94 | 1 | 94 | 2.73e-64 | 192 |
| MS.gene054412.t1 | MTR_4g085070 | 60.360 | 111 | 42 | 1 | 22 | 130 | 18 | 128 | 1.42e-41 | 136 |
| MS.gene054412.t1 | MTR_8g022310 | 45.113 | 133 | 66 | 4 | 8 | 134 | 2 | 133 | 1.49e-32 | 115 |
| MS.gene054412.t1 | MTR_8g022310 | 45.113 | 133 | 66 | 4 | 8 | 134 | 2 | 133 | 3.56e-32 | 114 |
| MS.gene054412.t1 | MTR_8g022310 | 45.113 | 133 | 66 | 4 | 8 | 134 | 2 | 133 | 4.60e-32 | 114 |
| MS.gene054412.t1 | MTR_8g022310 | 45.113 | 133 | 66 | 4 | 8 | 134 | 2 | 133 | 5.87e-32 | 114 |
| MS.gene054412.t1 | MTR_3g108020 | 53.030 | 66 | 28 | 1 | 17 | 82 | 5 | 67 | 1.14e-18 | 81.3 |
| MS.gene054412.t1 | MTR_2g090705 | 53.030 | 66 | 28 | 1 | 17 | 82 | 5 | 67 | 1.60e-17 | 78.2 |
| MS.gene054412.t1 | MTR_4g078850 | 48.485 | 66 | 31 | 1 | 17 | 82 | 5 | 67 | 6.19e-17 | 76.6 |
| MS.gene054412.t1 | MTR_2g014470 | 46.970 | 66 | 32 | 1 | 17 | 82 | 5 | 67 | 8.21e-17 | 76.3 |
| MS.gene054412.t1 | MTR_4g015840 | 53.030 | 66 | 28 | 1 | 17 | 82 | 5 | 67 | 1.39e-16 | 75.9 |
| MS.gene054412.t1 | MTR_4g109510 | 45.588 | 68 | 37 | 0 | 16 | 83 | 8 | 75 | 5.02e-16 | 74.7 |
| MS.gene054412.t1 | MTR_2g014480 | 48.485 | 66 | 31 | 1 | 17 | 82 | 5 | 67 | 5.29e-16 | 73.9 |
| MS.gene054412.t1 | MTR_1g110310 | 40.000 | 90 | 50 | 1 | 1 | 90 | 1 | 86 | 9.68e-16 | 73.6 |
| MS.gene054412.t1 | MTR_5g037880 | 44.286 | 70 | 35 | 1 | 17 | 82 | 6 | 75 | 5.15e-15 | 70.9 |
| MS.gene054412.t1 | MTR_7g101070 | 48.529 | 68 | 32 | 1 | 17 | 84 | 359 | 423 | 6.90e-15 | 71.2 |
| MS.gene054412.t1 | MTR_6g027110 | 44.776 | 67 | 36 | 1 | 17 | 82 | 3 | 69 | 7.51e-15 | 67.8 |
| MS.gene054412.t1 | MTR_3g087030 | 50.000 | 66 | 28 | 2 | 17 | 82 | 83 | 143 | 2.67e-14 | 69.7 |
| MS.gene054412.t1 | MTR_1g052110 | 44.737 | 76 | 38 | 2 | 7 | 82 | 29 | 100 | 6.08e-14 | 68.2 |
| MS.gene054412.t1 | MTR_7g092380 | 50.769 | 65 | 28 | 2 | 18 | 82 | 24 | 84 | 6.28e-14 | 67.8 |
| MS.gene054412.t1 | MTR_7g109340 | 45.333 | 75 | 37 | 2 | 8 | 82 | 18 | 88 | 2.00e-13 | 67.0 |
| MS.gene054412.t1 | MTR_8g081620 | 46.970 | 66 | 33 | 1 | 17 | 82 | 61 | 124 | 8.97e-13 | 63.5 |
| MS.gene054412.t1 | MTR_4g023470 | 48.485 | 66 | 30 | 1 | 17 | 82 | 87 | 148 | 1.39e-12 | 64.7 |
| MS.gene054412.t1 | MTR_6g026940 | 43.284 | 67 | 37 | 1 | 17 | 82 | 3 | 69 | 1.49e-12 | 62.8 |
| MS.gene054412.t1 | MTR_3g027580 | 46.970 | 66 | 31 | 1 | 17 | 82 | 90 | 151 | 1.77e-12 | 64.3 |
| MS.gene054412.t1 | MTR_6g027030 | 43.284 | 67 | 37 | 1 | 17 | 82 | 3 | 69 | 2.15e-12 | 62.8 |
| MS.gene054412.t1 | MTR_5g065160 | 43.939 | 66 | 33 | 1 | 17 | 82 | 83 | 144 | 3.80e-12 | 63.5 |
| MS.gene054412.t1 | MTR_5g065160 | 43.939 | 66 | 33 | 1 | 17 | 82 | 84 | 145 | 3.80e-12 | 63.5 |
| MS.gene054412.t1 | MTR_5g035770 | 37.975 | 79 | 45 | 1 | 18 | 96 | 27 | 101 | 4.03e-12 | 63.5 |
| MS.gene054412.t1 | MTR_5g065860 | 45.588 | 68 | 33 | 1 | 17 | 84 | 49 | 112 | 4.42e-12 | 60.8 |
| MS.gene054412.t1 | MTR_5g065160 | 43.939 | 66 | 33 | 1 | 17 | 82 | 77 | 138 | 4.66e-12 | 63.2 |
| MS.gene054412.t1 | MTR_5g065160 | 43.939 | 66 | 33 | 1 | 17 | 82 | 84 | 145 | 5.41e-12 | 63.2 |
| MS.gene054412.t1 | MTR_2g104030 | 46.377 | 69 | 32 | 2 | 14 | 82 | 68 | 131 | 1.09e-11 | 62.4 |
| MS.gene054412.t1 | MTR_8g037810 | 34.646 | 127 | 64 | 4 | 12 | 123 | 2 | 124 | 1.78e-11 | 61.2 |
| MS.gene054412.t1 | MTR_8g006430 | 41.333 | 75 | 36 | 1 | 17 | 91 | 15 | 81 | 2.34e-11 | 61.2 |
| MS.gene054412.t1 | MTR_6g003930 | 36.250 | 80 | 47 | 2 | 9 | 85 | 5 | 83 | 3.76e-11 | 58.9 |
| MS.gene054412.t1 | MTR_5g004570 | 43.056 | 72 | 37 | 1 | 12 | 83 | 2 | 69 | 5.15e-11 | 59.3 |
| MS.gene054412.t1 | MTR_4g076240 | 41.463 | 82 | 32 | 1 | 18 | 83 | 956 | 1037 | 7.04e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054412.t1 | AT1G56300 | 71.069 | 159 | 43 | 2 | 1 | 159 | 1 | 156 | 5.15e-72 | 214 |
| MS.gene054412.t1 | AT1G56300 | 77.679 | 112 | 22 | 2 | 1 | 112 | 1 | 109 | 2.87e-53 | 165 |
| MS.gene054412.t1 | AT1G71000 | 52.448 | 143 | 52 | 4 | 10 | 139 | 3 | 142 | 7.05e-41 | 135 |
| MS.gene054412.t1 | AT1G71000 | 44.654 | 159 | 79 | 4 | 10 | 163 | 3 | 157 | 8.86e-39 | 130 |
| MS.gene054412.t1 | AT3G14200 | 47.967 | 123 | 58 | 3 | 18 | 135 | 14 | 135 | 1.34e-32 | 116 |
| MS.gene054412.t1 | AT1G72416 | 40.816 | 98 | 56 | 2 | 18 | 115 | 7 | 102 | 1.73e-22 | 89.0 |
| MS.gene054412.t1 | AT1G72416 | 40.816 | 98 | 56 | 2 | 18 | 115 | 7 | 102 | 2.08e-22 | 88.6 |
| MS.gene054412.t1 | AT1G72416 | 40.816 | 98 | 56 | 2 | 18 | 115 | 7 | 102 | 3.44e-22 | 88.6 |
| MS.gene054412.t1 | AT1G72416 | 42.222 | 90 | 50 | 2 | 18 | 107 | 7 | 94 | 1.51e-21 | 86.7 |
| MS.gene054412.t1 | AT5G25530 | 37.594 | 133 | 67 | 3 | 17 | 149 | 5 | 121 | 1.83e-19 | 83.6 |
| MS.gene054412.t1 | AT1G10350 | 50.000 | 66 | 30 | 1 | 17 | 82 | 5 | 67 | 2.06e-17 | 78.2 |
| MS.gene054412.t1 | AT3G08910 | 53.030 | 66 | 28 | 1 | 17 | 82 | 5 | 67 | 2.74e-17 | 77.4 |
| MS.gene054412.t1 | AT4G28480 | 53.030 | 66 | 28 | 1 | 17 | 82 | 5 | 67 | 3.09e-17 | 77.0 |
| MS.gene054412.t1 | AT1G59725 | 52.239 | 67 | 28 | 2 | 17 | 82 | 5 | 68 | 4.55e-17 | 77.0 |
| MS.gene054412.t1 | AT4G28480 | 53.030 | 66 | 28 | 1 | 17 | 82 | 5 | 67 | 5.24e-17 | 77.0 |
| MS.gene054412.t1 | AT5G25530 | 35.461 | 141 | 67 | 4 | 17 | 149 | 5 | 129 | 1.03e-16 | 76.3 |
| MS.gene054412.t1 | AT2G20560 | 53.030 | 66 | 28 | 1 | 17 | 82 | 5 | 67 | 1.14e-16 | 75.9 |
| MS.gene054412.t1 | AT5G01390 | 50.000 | 66 | 30 | 1 | 17 | 82 | 5 | 67 | 2.59e-16 | 73.9 |
| MS.gene054412.t1 | AT5G01390 | 50.000 | 66 | 30 | 1 | 17 | 82 | 5 | 67 | 3.65e-16 | 73.9 |
| MS.gene054412.t1 | AT5G01390 | 50.000 | 66 | 30 | 1 | 17 | 82 | 5 | 67 | 3.96e-16 | 73.2 |
| MS.gene054412.t1 | AT5G01390 | 50.000 | 66 | 30 | 1 | 17 | 82 | 5 | 67 | 7.20e-16 | 73.9 |
| MS.gene054412.t1 | AT3G47940 | 49.254 | 67 | 30 | 2 | 17 | 82 | 5 | 68 | 3.40e-15 | 72.0 |
| MS.gene054412.t1 | AT2G33735 | 38.298 | 94 | 54 | 1 | 17 | 110 | 23 | 112 | 1.19e-14 | 66.6 |
| MS.gene054412.t1 | AT2G33735 | 38.298 | 94 | 54 | 1 | 17 | 110 | 23 | 112 | 1.19e-14 | 66.6 |
| MS.gene054412.t1 | AT2G41000 | 43.478 | 69 | 38 | 1 | 17 | 84 | 4 | 72 | 1.77e-14 | 67.8 |
| MS.gene054412.t1 | AT2G41000 | 43.478 | 69 | 38 | 1 | 17 | 84 | 4 | 72 | 2.60e-14 | 68.2 |
| MS.gene054412.t1 | AT5G16650 | 45.833 | 72 | 34 | 2 | 17 | 87 | 12 | 79 | 3.83e-14 | 65.9 |
| MS.gene054412.t1 | AT5G03160 | 47.059 | 68 | 33 | 1 | 17 | 84 | 371 | 435 | 4.49e-14 | 69.3 |
| MS.gene054412.t1 | AT1G24120 | 37.975 | 79 | 45 | 1 | 18 | 96 | 22 | 96 | 5.65e-14 | 68.9 |
| MS.gene054412.t1 | AT2G22360 | 48.485 | 66 | 29 | 2 | 17 | 82 | 87 | 147 | 5.69e-14 | 68.9 |
| MS.gene054412.t1 | AT5G16650 | 45.833 | 72 | 34 | 2 | 17 | 87 | 12 | 79 | 7.99e-14 | 65.9 |
| MS.gene054412.t1 | AT1G74250 | 43.478 | 69 | 38 | 1 | 16 | 83 | 11 | 79 | 1.96e-13 | 67.4 |
| MS.gene054412.t1 | AT3G12170 | 47.692 | 65 | 30 | 1 | 18 | 82 | 13 | 73 | 2.42e-13 | 66.2 |
| MS.gene054412.t1 | AT1G68370 | 42.466 | 73 | 38 | 1 | 18 | 90 | 19 | 87 | 2.51e-13 | 67.0 |
| MS.gene054412.t1 | AT5G06910 | 42.683 | 82 | 38 | 2 | 1 | 82 | 19 | 91 | 3.68e-13 | 65.9 |
| MS.gene054412.t1 | AT3G12170 | 47.692 | 65 | 30 | 1 | 18 | 82 | 49 | 109 | 7.82e-13 | 65.1 |
| MS.gene054412.t1 | AT4G39960 | 48.485 | 66 | 29 | 2 | 17 | 82 | 86 | 146 | 9.24e-13 | 65.5 |
| MS.gene054412.t1 | AT4G39960 | 48.485 | 66 | 29 | 2 | 17 | 82 | 86 | 146 | 9.24e-13 | 65.5 |
| MS.gene054412.t1 | AT3G62600 | 48.485 | 66 | 30 | 1 | 17 | 82 | 27 | 88 | 1.34e-12 | 64.7 |
| MS.gene054412.t1 | AT5G12430 | 46.154 | 91 | 27 | 4 | 18 | 92 | 1026 | 1110 | 3.99e-12 | 63.5 |
| MS.gene054412.t1 | AT1G59980 | 38.462 | 78 | 44 | 1 | 6 | 83 | 13 | 86 | 4.56e-12 | 63.2 |
| MS.gene054412.t1 | AT1G59980 | 38.462 | 78 | 44 | 1 | 6 | 83 | 13 | 86 | 6.18e-12 | 62.8 |
| MS.gene054412.t1 | AT5G48030 | 38.667 | 75 | 42 | 1 | 17 | 91 | 95 | 165 | 1.09e-11 | 62.4 |
| MS.gene054412.t1 | AT2G21510 | 40.845 | 71 | 38 | 1 | 12 | 82 | 2 | 68 | 1.38e-11 | 61.6 |
| MS.gene054412.t1 | AT2G21510 | 40.845 | 71 | 38 | 1 | 12 | 82 | 2 | 68 | 1.38e-11 | 61.6 |
| MS.gene054412.t1 | AT2G21510 | 40.845 | 71 | 38 | 1 | 12 | 82 | 2 | 68 | 1.63e-11 | 61.6 |
| MS.gene054412.t1 | AT2G21510 | 40.845 | 71 | 38 | 1 | 12 | 82 | 2 | 68 | 1.63e-11 | 61.6 |
| MS.gene054412.t1 | AT2G21510 | 40.845 | 71 | 38 | 1 | 12 | 82 | 2 | 68 | 1.78e-11 | 61.2 |
| MS.gene054412.t1 | AT4G39150 | 42.254 | 71 | 37 | 1 | 12 | 82 | 2 | 68 | 2.69e-11 | 60.8 |
| MS.gene054412.t1 | AT4G39150 | 42.254 | 71 | 37 | 1 | 12 | 82 | 2 | 68 | 2.69e-11 | 60.8 |
| MS.gene054412.t1 | AT4G39150 | 42.254 | 71 | 37 | 1 | 12 | 82 | 2 | 68 | 2.69e-11 | 60.8 |
| MS.gene054412.t1 | AT1G80030 | 42.424 | 66 | 33 | 2 | 17 | 82 | 76 | 136 | 2.92e-11 | 60.8 |
| MS.gene054412.t1 | AT1G80030 | 42.424 | 66 | 33 | 2 | 17 | 82 | 76 | 136 | 3.21e-11 | 60.8 |
| MS.gene054412.t1 | AT1G80030 | 42.424 | 66 | 33 | 2 | 17 | 82 | 76 | 136 | 3.21e-11 | 60.8 |
| MS.gene054412.t1 | AT1G80030 | 42.424 | 66 | 33 | 2 | 17 | 82 | 76 | 136 | 3.21e-11 | 60.8 |
| MS.gene054412.t1 | AT1G28210 | 40.000 | 70 | 38 | 1 | 17 | 86 | 49 | 114 | 4.22e-11 | 60.5 |
| MS.gene054412.t1 | AT1G28210 | 40.000 | 70 | 38 | 1 | 17 | 86 | 49 | 114 | 4.55e-11 | 60.5 |
| MS.gene054412.t1 | AT1G28210 | 40.000 | 70 | 38 | 1 | 17 | 86 | 49 | 114 | 5.05e-11 | 60.5 |
Find 36 sgRNAs with CRISPR-Local
Find 77 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTGCTATTACTCTGTTCT+TGG | 0.198681 | 7.4:-4339895 | MS.gene054412:CDS |
| GGATTTCGAGCGAATTTATC+AGG | 0.209743 | 7.4:+4339709 | None:intergenic |
| CAAATTCAAGAAGCATATTC+AGG | 0.256213 | 7.4:-4339652 | MS.gene054412:intron |
| TTGGCTTCTCCGGCAGTTGT+AGG | 0.330200 | 7.4:+4339688 | None:intergenic |
| TCTTCCCAGCCGTCTGATCT+TGG | 0.344100 | 7.4:+4338805 | None:intergenic |
| GCGGCAATGATTGCTGCGCT+TGG | 0.404589 | 7.4:+4338749 | None:intergenic |
| AATTTGCTGAAACCGTCGTT+TGG | 0.414220 | 7.4:+4339669 | None:intergenic |
| GTTGAGATGGTAGGTGGAGA+TGG | 0.451612 | 7.4:-4338846 | MS.gene054412:CDS |
| ACAAAGGATGTTTGTTGAGA+TGG | 0.470153 | 7.4:-4338859 | MS.gene054412:CDS |
| AAACCGTCGTTTGGCTTCTC+CGG | 0.481969 | 7.4:+4339678 | None:intergenic |
| TTGAACCAAGATCAGACGGC+TGG | 0.499992 | 7.4:-4338810 | MS.gene054412:CDS |
| AAAAGATCAATGTACGATGC+TGG | 0.500396 | 7.4:-4339519 | MS.gene054412:CDS |
| TGCTTACCGCAAACTCGCTA+TGG | 0.507423 | 7.4:-4339836 | MS.gene054412:intron |
| CGTTTACCATAGCGAGTTTG+CGG | 0.522874 | 7.4:+4339830 | None:intergenic |
| GATCAGACGGCTGGGAAGAG+AGG | 0.537894 | 7.4:-4338801 | MS.gene054412:CDS |
| AGTGCGAATATCAGAGGAGG+AGG | 0.553290 | 7.4:+4339858 | None:intergenic |
| ACTTGGTCTTCTTCTTCAAG+AGG | 0.556126 | 7.4:+4339486 | None:intergenic |
| GCTCGAAATCCTACAACTGC+CGG | 0.560164 | 7.4:-4339697 | MS.gene054412:CDS |
| GGGAAGAGAGGACGCTCAAG+TGG | 0.569154 | 7.4:-4338789 | MS.gene054412:CDS |
| AGAAGATCTAATACCACCGT+TGG | 0.578456 | 7.4:+4339915 | None:intergenic |
| TGATTTGAACCAAGATCAGA+CGG | 0.588129 | 7.4:-4338814 | MS.gene054412:CDS |
| GATGAATAATGTCAAAGATG+AGG | 0.606591 | 7.4:-4339033 | MS.gene054412:intron |
| GATTTCGAGCGAATTTATCA+GGG | 0.606606 | 7.4:+4339710 | None:intergenic |
| TGAACCAAGATCAGACGGCT+GGG | 0.607458 | 7.4:-4338809 | MS.gene054412:CDS |
| CAAGAAGGAGGAGGATCCAA+CGG | 0.620400 | 7.4:-4339931 | MS.gene054412:CDS |
| GGACGCTCAAGTGGATCAAG+AGG | 0.624597 | 7.4:-4338780 | MS.gene054412:CDS |
| ATGTTTGTTGAGATGGTAGG+TGG | 0.628226 | 7.4:-4338852 | MS.gene054412:CDS |
| GTAAGCAGTGCGAATATCAG+AGG | 0.644250 | 7.4:+4339852 | None:intergenic |
| CCAACGGTGATGGATCAAGA+AGG | 0.647408 | 7.4:-4339946 | None:intergenic |
| AGGATGTTTGTTGAGATGGT+AGG | 0.652987 | 7.4:-4338855 | MS.gene054412:CDS |
| AGCAGTGCGAATATCAGAGG+AGG | 0.654808 | 7.4:+4339855 | None:intergenic |
| GTGATGGATCAAGAAGGAGG+AGG | 0.667540 | 7.4:-4339940 | MS.gene054412:CDS |
| ACGGTGATGGATCAAGAAGG+AGG | 0.684880 | 7.4:-4339943 | None:intergenic |
| ACAGTTTGGAGGATCTACAA+AGG | 0.691000 | 7.4:-4338875 | MS.gene054412:CDS |
| CTGCCGGAGAAGCCAAACGA+CGG | 0.702205 | 7.4:-4339681 | MS.gene054412:CDS |
| GAAGGAGGAGGATCCAACGG+TGG | 0.737111 | 7.4:-4339928 | MS.gene054412:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTAAAATAAAAATTAAAAA+AGG | + | chr7.4:4339384-4339403 | None:intergenic | 0.0% |
| !!! | ATTTATTTATTTAAAGTGAA+TGG | + | chr7.4:4338912-4338931 | None:intergenic | 10.0% |
| !!! | ATTTTTTTTTTTACCAAAAA+AGG | + | chr7.4:4339700-4339719 | None:intergenic | 10.0% |
| !! | TTTGGTAAAAAAAAAAATGA+AGG | - | chr7.4:4339702-4339721 | MS.gene054412:CDS | 15.0% |
| !!! | AAATTCAAATTTTCTATAGA+TGG | - | chr7.4:4339062-4339081 | MS.gene054412:CDS | 15.0% |
| !! | AATATAAATGAGTAAAACGA+CGG | + | chr7.4:4338879-4338898 | None:intergenic | 20.0% |
| !! | ATATGAGATTGATTATTGAT+TGG | - | chr7.4:4339101-4339120 | MS.gene054412:intron | 20.0% |
| !!! | TGGATTCTAATTCTAATTTT+AGG | - | chr7.4:4339568-4339587 | MS.gene054412:intron | 20.0% |
| ! | AAAAATATCTTGTGCATGTT+TGG | - | chr7.4:4339416-4339435 | MS.gene054412:intron | 25.0% |
| ! | ATTAAAAAAGGCAAAGCAAA+TGG | + | chr7.4:4339372-4339391 | None:intergenic | 25.0% |
| ! | CAAAATTTGTGTTATAATGC+AGG | - | chr7.4:4339593-4339612 | MS.gene054412:intron | 25.0% |
| ! | TTAAAAAAGGCAAAGCAAAT+GGG | + | chr7.4:4339371-4339390 | None:intergenic | 25.0% |
| ! | TTGTTGAATTTGAAAAGAGA+TGG | - | chr7.4:4338948-4338967 | MS.gene054412:intron | 25.0% |
| !! | TTCTATAGATGGTAATGAAT+TGG | - | chr7.4:4339073-4339092 | MS.gene054412:intron | 25.0% |
| !!! | GTTCTAATTGTTTCCTTTTT+TGG | - | chr7.4:4339684-4339703 | MS.gene054412:CDS | 25.0% |
| !!! | TGGTATTTTCTGGTTATTTA+GGG | - | chr7.4:4339783-4339802 | MS.gene054412:intron | 25.0% |
| !!! | TTGATTGGTTTTAATGTTGT+TGG | - | chr7.4:4339116-4339135 | MS.gene054412:intron | 25.0% |
| !!! | TTGGTATTTTCTGGTTATTT+AGG | - | chr7.4:4339782-4339801 | MS.gene054412:intron | 25.0% |
| AAGAAGAATTGTTACGAACT+TGG | + | chr7.4:4339217-4339236 | None:intergenic | 30.0% | |
| ACAAGACGAGAAAAACAATA+GGG | + | chr7.4:4339328-4339347 | None:intergenic | 30.0% | |
| AGTTAGGTGCATTTGAATAT+TGG | - | chr7.4:4339548-4339567 | MS.gene054412:intron | 30.0% | |
| CAAAGATGAGGTATACTATT+GGG | - | chr7.4:4339662-4339681 | MS.gene054412:CDS | 30.0% | |
| GATGAATAATGTCAAAGATG+AGG | - | chr7.4:4339650-4339669 | MS.gene054412:intron | 30.0% | |
| TCAAAGATGAGGTATACTAT+TGG | - | chr7.4:4339661-4339680 | MS.gene054412:CDS | 30.0% | |
| ! | AAACACGTGATTTTGTTGAA+AGG | - | chr7.4:4339448-4339467 | MS.gene054412:intron | 30.0% |
| ! | ATGTATTGTGATGATGTGAT+GGG | - | chr7.4:4339754-4339773 | MS.gene054412:intron | 30.0% |
| ! | CAAATTCAAGAAGCATATTC+AGG | - | chr7.4:4339031-4339050 | MS.gene054412:intron | 30.0% |
| !!! | GGTATTTTCTGGTTATTTAG+GGG | - | chr7.4:4339784-4339803 | MS.gene054412:intron | 30.0% |
| !!! | GTATTTTCTGGTTATTTAGG+GGG | - | chr7.4:4339785-4339804 | MS.gene054412:intron | 30.0% |
| !!! | GTTTTACCTCGTTTTGTTTA+CGG | + | chr7.4:4339281-4339300 | None:intergenic | 30.0% |
| AAAAGATCAATGTACGATGC+TGG | - | chr7.4:4339164-4339183 | MS.gene054412:intron | 35.0% | |
| ACAAAATCACGTGTTTACAG+TGG | + | chr7.4:4339444-4339463 | None:intergenic | 35.0% | |
| CACAAGACGAGAAAAACAAT+AGG | + | chr7.4:4339329-4339348 | None:intergenic | 35.0% | |
| GATTTCGAGCGAATTTATCA+GGG | + | chr7.4:4338976-4338995 | None:intergenic | 35.0% | |
| TGATTTGAACCAAGATCAGA+CGG | - | chr7.4:4339869-4339888 | MS.gene054412:CDS | 35.0% | |
| ! | ACAAAGGATGTTTGTTGAGA+TGG | - | chr7.4:4339824-4339843 | MS.gene054412:intron | 35.0% |
| ! | ATTTTGCCGTAAACAAAACG+AGG | - | chr7.4:4339272-4339291 | MS.gene054412:intron | 35.0% |
| ! | GATGTATTGTGATGATGTGA+TGG | - | chr7.4:4339753-4339772 | MS.gene054412:intron | 35.0% |
| ! | TGTATTGTGATGATGTGATG+GGG | - | chr7.4:4339755-4339774 | MS.gene054412:intron | 35.0% |
| ! | TGTTTTTCTCGTCTTGTGTT+CGG | - | chr7.4:4339331-4339350 | MS.gene054412:intron | 35.0% |
| !! | TCTTGCTATTACTCTGTTCT+TGG | - | chr7.4:4338788-4338807 | MS.gene054412:CDS | 35.0% |
| !!! | TGGGGTATTTTGGTATTTTC+TGG | - | chr7.4:4339773-4339792 | MS.gene054412:intron | 35.0% |
| ACAGTTTGGAGGATCTACAA+AGG | - | chr7.4:4339808-4339827 | MS.gene054412:intron | 40.0% | |
| AGAAAAACAATAGGGTCGTC+TGG | + | chr7.4:4339320-4339339 | None:intergenic | 40.0% | |
| AGAAGATCTAATACCACCGT+TGG | + | chr7.4:4338771-4338790 | None:intergenic | 40.0% | |
| GGATTTCGAGCGAATTTATC+AGG | + | chr7.4:4338977-4338996 | None:intergenic | 40.0% | |
| ! | ACTTGGTCTTCTTCTTCAAG+AGG | + | chr7.4:4339200-4339219 | None:intergenic | 40.0% |
| ! | AGGATGTTTGTTGAGATGGT+AGG | - | chr7.4:4339828-4339847 | MS.gene054412:intron | 40.0% |
| ! | ATGTTTGTTGAGATGGTAGG+TGG | - | chr7.4:4339831-4339850 | MS.gene054412:intron | 40.0% |
| !! | AATTTGCTGAAACCGTCGTT+TGG | + | chr7.4:4339017-4339036 | None:intergenic | 40.0% |
| !! | GATGATGTGATGGGGTATTT+TGG | - | chr7.4:4339763-4339782 | MS.gene054412:intron | 40.0% |
| !!! | GAGGTAAAACTGTGTCGTTT+TGG | - | chr7.4:4339291-4339310 | MS.gene054412:intron | 40.0% |
| CGTTTACCATAGCGAGTTTG+CGG | + | chr7.4:4338856-4338875 | None:intergenic | 45.0% | |
| CTTGTAGTGCAGCTCAAGTT+AGG | - | chr7.4:4339532-4339551 | MS.gene054412:CDS | 45.0% | |
| GGTTATTTAGGGGGACAGTT+TGG | - | chr7.4:4339794-4339813 | MS.gene054412:intron | 45.0% | |
| GTAAGCAGTGCGAATATCAG+AGG | + | chr7.4:4338834-4338853 | None:intergenic | 45.0% | |
| TATTTAGGGGGACAGTTTGG+AGG | - | chr7.4:4339797-4339816 | MS.gene054412:intron | 45.0% | |
| TCGTTTCAGTTGCAGAGCAT+GGG | - | chr7.4:4339238-4339257 | MS.gene054412:intron | 45.0% | |
| TTCGTTTCAGTTGCAGAGCA+TGG | - | chr7.4:4339237-4339256 | MS.gene054412:intron | 45.0% | |
| AGCAGTGCGAATATCAGAGG+AGG | + | chr7.4:4338831-4338850 | None:intergenic | 50.0% | |
| AGTGCGAATATCAGAGGAGG+AGG | + | chr7.4:4338828-4338847 | None:intergenic | 50.0% | |
| CAAGAAGGAGGAGGATCCAA+CGG | - | chr7.4:4338752-4338771 | MS.gene054412:CDS | 50.0% | |
| CGTTTCAGTTGCAGAGCATG+GGG | - | chr7.4:4339239-4339258 | MS.gene054412:intron | 50.0% | |
| GCTCGAAATCCTACAACTGC+CGG | - | chr7.4:4338986-4339005 | MS.gene054412:intron | 50.0% | |
| GTTGAGATGGTAGGTGGAGA+TGG | - | chr7.4:4339837-4339856 | MS.gene054412:CDS | 50.0% | |
| TGAACCAAGATCAGACGGCT+GGG | - | chr7.4:4339874-4339893 | MS.gene054412:CDS | 50.0% | |
| TGCTTACCGCAAACTCGCTA+TGG | - | chr7.4:4338847-4338866 | MS.gene054412:CDS | 50.0% | |
| TTGAACCAAGATCAGACGGC+TGG | - | chr7.4:4339873-4339892 | MS.gene054412:CDS | 50.0% | |
| ! | AAACCGTCGTTTGGCTTCTC+CGG | + | chr7.4:4339008-4339027 | None:intergenic | 50.0% |
| GGACGCTCAAGTGGATCAAG+AGG | - | chr7.4:4339903-4339922 | MS.gene054412:CDS | 55.0% | |
| TCTTCCCAGCCGTCTGATCT+TGG | + | chr7.4:4339881-4339900 | None:intergenic | 55.0% | |
| TTGGCTTCTCCGGCAGTTGT+AGG | + | chr7.4:4338998-4339017 | None:intergenic | 55.0% | |
| CTGCCGGAGAAGCCAAACGA+CGG | - | chr7.4:4339002-4339021 | MS.gene054412:intron | 60.0% | |
| GAAGGAGGAGGATCCAACGG+TGG | - | chr7.4:4338755-4338774 | MS.gene054412:CDS | 60.0% | |
| GATCAGACGGCTGGGAAGAG+AGG | - | chr7.4:4339882-4339901 | MS.gene054412:CDS | 60.0% | |
| GCGGCAATGATTGCTGCGCT+TGG | + | chr7.4:4339937-4339956 | None:intergenic | 60.0% | |
| GGGAAGAGAGGACGCTCAAG+TGG | - | chr7.4:4339894-4339913 | MS.gene054412:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 4338746 | 4339959 | 4338746 | ID=MS.gene054412 |
| chr7.4 | mRNA | 4338746 | 4339959 | 4338746 | ID=MS.gene054412.t1;Parent=MS.gene054412 |
| chr7.4 | exon | 4339837 | 4339959 | 4339837 | ID=MS.gene054412.t1.exon1;Parent=MS.gene054412.t1 |
| chr7.4 | CDS | 4339837 | 4339959 | 4339837 | ID=cds.MS.gene054412.t1;Parent=MS.gene054412.t1 |
| chr7.4 | exon | 4339653 | 4339740 | 4339653 | ID=MS.gene054412.t1.exon2;Parent=MS.gene054412.t1 |
| chr7.4 | CDS | 4339653 | 4339740 | 4339653 | ID=cds.MS.gene054412.t1;Parent=MS.gene054412.t1 |
| chr7.4 | exon | 4339488 | 4339558 | 4339488 | ID=MS.gene054412.t1.exon3;Parent=MS.gene054412.t1 |
| chr7.4 | CDS | 4339488 | 4339558 | 4339488 | ID=cds.MS.gene054412.t1;Parent=MS.gene054412.t1 |
| chr7.4 | exon | 4339034 | 4339090 | 4339034 | ID=MS.gene054412.t1.exon4;Parent=MS.gene054412.t1 |
| chr7.4 | CDS | 4339034 | 4339090 | 4339034 | ID=cds.MS.gene054412.t1;Parent=MS.gene054412.t1 |
| chr7.4 | exon | 4338746 | 4338901 | 4338746 | ID=MS.gene054412.t1.exon5;Parent=MS.gene054412.t1 |
| chr7.4 | CDS | 4338746 | 4338901 | 4338746 | ID=cds.MS.gene054412.t1;Parent=MS.gene054412.t1 |
| Gene Sequence |
| Protein sequence |
