Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73497.t1 | XP_003617613.1 | 84.6 | 228 | 32 | 3 | 1 | 226 | 1 | 227 | 5.90E-104 | 387.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73497.t1 | Q9SJ74 | 28.2 | 206 | 129 | 7 | 16 | 210 | 4 | 201 | 4.6e-12 | 73.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73497.t1 | G7K8L9 | 84.6 | 228 | 32 | 3 | 1 | 226 | 1 | 227 | 4.3e-104 | 387.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052732 | MS.gene73497 | 0.829633 | 4.61E-55 | -1.69E-46 |
| MS.gene053144 | MS.gene73497 | 0.802333 | 5.90E-49 | -1.69E-46 |
| MS.gene054584 | MS.gene73497 | 0.802022 | 6.83E-49 | -1.69E-46 |
| MS.gene055144 | MS.gene73497 | 0.827641 | 1.40E-54 | -1.69E-46 |
| MS.gene058339 | MS.gene73497 | 0.808344 | 3.24E-50 | -1.69E-46 |
| MS.gene058909 | MS.gene73497 | 0.818445 | 1.95E-52 | -1.69E-46 |
| MS.gene058912 | MS.gene73497 | 0.81459 | 1.43E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73497.t1 | MTR_5g093520 | 84.649 | 228 | 32 | 3 | 1 | 226 | 1 | 227 | 2.43e-134 | 385 |
| MS.gene73497.t1 | MTR_8g011650 | 27.642 | 246 | 153 | 10 | 6 | 242 | 1 | 230 | 3.43e-15 | 72.8 |
| MS.gene73497.t1 | MTR_7g051910 | 28.283 | 198 | 126 | 8 | 11 | 208 | 2 | 183 | 1.86e-13 | 69.3 |
| MS.gene73497.t1 | MTR_8g009880 | 28.700 | 223 | 137 | 9 | 16 | 226 | 1 | 213 | 2.82e-12 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73497.t1 | AT2G04850 | 29.213 | 178 | 106 | 7 | 45 | 210 | 32 | 201 | 8.67e-12 | 64.7 |
| MS.gene73497.t1 | AT3G25290 | 28.834 | 163 | 103 | 6 | 50 | 208 | 42 | 195 | 5.09e-11 | 62.4 |
| MS.gene73497.t1 | AT3G25290 | 28.834 | 163 | 103 | 6 | 50 | 208 | 42 | 195 | 5.09e-11 | 62.4 |
Find 69 sgRNAs with CRISPR-Local
Find 71 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATAGTAGCAAAAGAGAAAA+AGG | 0.282441 | 5.2:+78657147 | None:intergenic |
| TTTCTCTTCTTTGCAAATAT+CGG | 0.320804 | 5.2:-78657773 | MS.gene73497:CDS |
| GCAAGTTGGGAAGGCTGTTA+GGG | 0.324027 | 5.2:-78657294 | MS.gene73497:CDS |
| GGCAAGTTGGGAAGGCTGTT+AGG | 0.324323 | 5.2:-78657295 | MS.gene73497:CDS |
| TGTTAGGGGTGATATACCTT+TGG | 0.324418 | 5.2:-78657279 | MS.gene73497:CDS |
| ACAATACTTGAAATCTTATT+CGG | 0.359172 | 5.2:-78657617 | MS.gene73497:CDS |
| TCTATATTGTGAATATTTGT+TGG | 0.359173 | 5.2:+78657251 | None:intergenic |
| CTGTCCCTTGCTGCGACCAT+CGG | 0.365418 | 5.2:+78657199 | None:intergenic |
| CCCATCCCCTTGATCCATAT+TGG | 0.365575 | 5.2:+78657592 | None:intergenic |
| AATCTTATTCGGTGCCAATA+TGG | 0.376709 | 5.2:-78657606 | MS.gene73497:CDS |
| ATTGGAACAAAGGCAATCAT+AGG | 0.380791 | 5.2:-78657527 | MS.gene73497:CDS |
| TAACAAGGTTGAACCATGTT+TGG | 0.384579 | 5.2:-78657316 | MS.gene73497:CDS |
| GTTAGGGGTGATATACCTTT+GGG | 0.389697 | 5.2:-78657278 | MS.gene73497:CDS |
| GCGGTAACTCCAACCGAATT+CGG | 0.389742 | 5.2:+78657658 | None:intergenic |
| ATCATTTCAGCCCTTTCCCC+TGG | 0.395994 | 5.2:+78657548 | None:intergenic |
| TAAAAGCCTACAACCCTGTT+TGG | 0.402063 | 5.2:+78657448 | None:intergenic |
| TGAACCATGTTTGGCAAGTT+GGG | 0.406735 | 5.2:-78657307 | MS.gene73497:CDS |
| CGAACTCTAGGAGCCGAATT+CGG | 0.413289 | 5.2:-78657671 | MS.gene73497:CDS |
| AAGGAACCAAACAGGGTTGT+AGG | 0.414976 | 5.2:-78657454 | MS.gene73497:CDS |
| GGTTCCTTTAGTAACATCAT+AGG | 0.424605 | 5.2:+78657469 | None:intergenic |
| GGGGAAAGGGCTGAAATGAT+TGG | 0.432198 | 5.2:-78657545 | MS.gene73497:CDS |
| TTGAACCATGTTTGGCAAGT+TGG | 0.443630 | 5.2:-78657308 | MS.gene73497:CDS |
| TGTTACTAAAGGAACCAAAC+AGG | 0.445031 | 5.2:-78657462 | MS.gene73497:CDS |
| CACGTTAAAGCCAATATCAT+TGG | 0.452463 | 5.2:+78657421 | None:intergenic |
| GAGAAAGGACAATATCATAA+TGG | 0.453788 | 5.2:+78657790 | None:intergenic |
| TGTCCCTTGCTGCGACCATC+GGG | 0.455324 | 5.2:+78657200 | None:intergenic |
| GGGTGTAAATCCAGGGGAAA+GGG | 0.460004 | 5.2:-78657558 | MS.gene73497:CDS |
| CTCTAGGAGCCGAATTCGGT+TGG | 0.468915 | 5.2:-78657667 | MS.gene73497:CDS |
| AAGGGGATGGGTGGATAGCA+TGG | 0.470518 | 5.2:-78657580 | MS.gene73497:CDS |
| ATAGCATGGGGTGTAAATCC+AGG | 0.477364 | 5.2:-78657566 | MS.gene73497:CDS |
| GCCAATATGGATCAAGGGGA+TGG | 0.480674 | 5.2:-78657593 | MS.gene73497:CDS |
| TTCGGTGCCAATATGGATCA+AGG | 0.487774 | 5.2:-78657599 | MS.gene73497:CDS |
| CAATATCATAATGGTTACTA+AGG | 0.488331 | 5.2:+78657799 | None:intergenic |
| GATATGTCGATTCAAGATGA+AGG | 0.502397 | 5.2:-78657395 | MS.gene73497:CDS |
| AGGGGATGGGTGGATAGCAT+GGG | 0.510652 | 5.2:-78657579 | MS.gene73497:CDS |
| TATTGTGAATATTTGTTGGA+TGG | 0.512465 | 5.2:+78657255 | None:intergenic |
| GTTATGTTATATTTATCAGA+AGG | 0.514306 | 5.2:+78657335 | None:intergenic |
| CCTTCCCAACTTGCCAAACA+TGG | 0.528715 | 5.2:+78657303 | None:intergenic |
| GGGGTGTAAATCCAGGGGAA+AGG | 0.530797 | 5.2:-78657559 | MS.gene73497:CDS |
| GAATAAGATTTCAAGTATTG+TGG | 0.534057 | 5.2:+78657619 | None:intergenic |
| TTATCGCTGCATGGTTGTTG+AGG | 0.534691 | 5.2:+78657743 | None:intergenic |
| AAAACCTATGATGTTACTAA+AGG | 0.534733 | 5.2:-78657473 | MS.gene73497:CDS |
| ATCATAGGGATCAAATATCA+TGG | 0.537405 | 5.2:-78657512 | MS.gene73497:CDS |
| GAAACTGAGTTTCCTTCGAT+CGG | 0.542803 | 5.2:-78657177 | MS.gene73497:CDS |
| GATATTTGCAAAGAAGAGAA+AGG | 0.546213 | 5.2:+78657775 | None:intergenic |
| GGCTGAAATGATTGGAACAA+AGG | 0.548087 | 5.2:-78657537 | MS.gene73497:CDS |
| CCAATATGGATCAAGGGGAT+GGG | 0.551351 | 5.2:-78657592 | MS.gene73497:CDS |
| TGCAAAACATTACGAACTCT+AGG | 0.556387 | 5.2:-78657683 | MS.gene73497:CDS |
| TTCGCGAAGTTATCGCTGCA+TGG | 0.557211 | 5.2:+78657734 | None:intergenic |
| ATATGGATCAAGGGGATGGG+TGG | 0.557364 | 5.2:-78657589 | MS.gene73497:CDS |
| CTGATAAATATAACATAACA+AGG | 0.566580 | 5.2:-78657331 | MS.gene73497:CDS |
| TTCTCCCGATGGTCGCAGCA+AGG | 0.567113 | 5.2:-78657204 | MS.gene73497:CDS |
| TCTCCCGATGGTCGCAGCAA+GGG | 0.568984 | 5.2:-78657203 | MS.gene73497:CDS |
| TAGCATGGGGTGTAAATCCA+GGG | 0.574940 | 5.2:-78657565 | MS.gene73497:CDS |
| TCGGTGCCAATATGGATCAA+GGG | 0.576863 | 5.2:-78657598 | MS.gene73497:CDS |
| CCATGTTTGGCAAGTTGGGA+AGG | 0.580201 | 5.2:-78657303 | MS.gene73497:CDS |
| AAAGGATTGTTACCGATCGA+AGG | 0.580655 | 5.2:+78657165 | None:intergenic |
| GGGGATGGGTGGATAGCATG+GGG | 0.582355 | 5.2:-78657578 | MS.gene73497:CDS |
| TTATGTTATATTTATCAGAA+GGG | 0.582589 | 5.2:+78657336 | None:intergenic |
| TTGGAACAAAGGCAATCATA+GGG | 0.588140 | 5.2:-78657526 | MS.gene73497:CDS |
| ATATTTGTTGGATGGCCCAA+AGG | 0.598917 | 5.2:+78657263 | None:intergenic |
| CGGTGCCAATATGGATCAAG+GGG | 0.603107 | 5.2:-78657597 | MS.gene73497:CDS |
| CAAGTTGGGAAGGCTGTTAG+GGG | 0.623437 | 5.2:-78657293 | MS.gene73497:CDS |
| ATATGTCGATTCAAGATGAA+GGG | 0.624440 | 5.2:-78657394 | MS.gene73497:CDS |
| TGGTTGAGTTTGTGCCGTTG+CGG | 0.633100 | 5.2:+78657639 | None:intergenic |
| GTTACTAAAGGAACCAAACA+GGG | 0.664990 | 5.2:-78657461 | MS.gene73497:CDS |
| TTCGGTTGGAGTTACCGCAA+CGG | 0.679084 | 5.2:-78657653 | MS.gene73497:CDS |
| ATAGACTTGACTTCTCCCGA+TGG | 0.700298 | 5.2:-78657215 | MS.gene73497:CDS |
| AGCATGGGGTGTAAATCCAG+GGG | 0.707308 | 5.2:-78657564 | MS.gene73497:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATGTTATATTTATCAGAA+GGG | + | chr5.2:78657621-78657640 | None:intergenic | 15.0% |
| !! | ACAATACTTGAAATCTTATT+CGG | - | chr5.2:78657337-78657356 | MS.gene73497:CDS | 20.0% |
| !! | CTGATAAATATAACATAACA+AGG | - | chr5.2:78657623-78657642 | MS.gene73497:CDS | 20.0% |
| !! | GTTATGTTATATTTATCAGA+AGG | + | chr5.2:78657622-78657641 | None:intergenic | 20.0% |
| !! | TCTATATTGTGAATATTTGT+TGG | + | chr5.2:78657706-78657725 | None:intergenic | 20.0% |
| ! | AAAACCTATGATGTTACTAA+AGG | - | chr5.2:78657481-78657500 | MS.gene73497:CDS | 25.0% |
| ! | CAATATCATAATGGTTACTA+AGG | + | chr5.2:78657158-78657177 | None:intergenic | 25.0% |
| ! | TTTCTCTTCTTTGCAAATAT+CGG | - | chr5.2:78657181-78657200 | MS.gene73497:CDS | 25.0% |
| !! | CTTTTATCTACCAATGATAT+TGG | - | chr5.2:78657523-78657542 | MS.gene73497:CDS | 25.0% |
| !! | GAATAAGATTTCAAGTATTG+TGG | + | chr5.2:78657338-78657357 | None:intergenic | 25.0% |
| !! | TATTGTGAATATTTGTTGGA+TGG | + | chr5.2:78657702-78657721 | None:intergenic | 25.0% |
| ATCATAGGGATCAAATATCA+TGG | - | chr5.2:78657442-78657461 | MS.gene73497:CDS | 30.0% | |
| CATAGTAGCAAAAGAGAAAA+AGG | + | chr5.2:78657810-78657829 | None:intergenic | 30.0% | |
| GAGAAAGGACAATATCATAA+TGG | + | chr5.2:78657167-78657186 | None:intergenic | 30.0% | |
| GATATTTGCAAAGAAGAGAA+AGG | + | chr5.2:78657182-78657201 | None:intergenic | 30.0% | |
| !! | ATATGTCGATTCAAGATGAA+GGG | - | chr5.2:78657560-78657579 | MS.gene73497:CDS | 30.0% |
| !! | GTAACATCATAGGTTTTAAC+TGG | + | chr5.2:78657478-78657497 | None:intergenic | 30.0% |
| ATTGGAACAAAGGCAATCAT+AGG | - | chr5.2:78657427-78657446 | MS.gene73497:CDS | 35.0% | |
| CACGTTAAAGCCAATATCAT+TGG | + | chr5.2:78657536-78657555 | None:intergenic | 35.0% | |
| GGTTCCTTTAGTAACATCAT+AGG | + | chr5.2:78657488-78657507 | None:intergenic | 35.0% | |
| GTTACTAAAGGAACCAAACA+GGG | - | chr5.2:78657493-78657512 | MS.gene73497:CDS | 35.0% | |
| TAACAAGGTTGAACCATGTT+TGG | - | chr5.2:78657638-78657657 | MS.gene73497:CDS | 35.0% | |
| TGCAAAACATTACGAACTCT+AGG | - | chr5.2:78657271-78657290 | MS.gene73497:CDS | 35.0% | |
| TGTTACTAAAGGAACCAAAC+AGG | - | chr5.2:78657492-78657511 | MS.gene73497:CDS | 35.0% | |
| TTGGAACAAAGGCAATCATA+GGG | - | chr5.2:78657428-78657447 | MS.gene73497:CDS | 35.0% | |
| !! | AATCTTATTCGGTGCCAATA+TGG | - | chr5.2:78657348-78657367 | MS.gene73497:CDS | 35.0% |
| !! | GATATGTCGATTCAAGATGA+AGG | - | chr5.2:78657559-78657578 | MS.gene73497:CDS | 35.0% |
| AAAGGATTGTTACCGATCGA+AGG | + | chr5.2:78657792-78657811 | None:intergenic | 40.0% | |
| GAAACTGAGTTTCCTTCGAT+CGG | - | chr5.2:78657777-78657796 | MS.gene73497:CDS | 40.0% | |
| GGCTGAAATGATTGGAACAA+AGG | - | chr5.2:78657417-78657436 | MS.gene73497:CDS | 40.0% | |
| GTTAGGGGTGATATACCTTT+GGG | - | chr5.2:78657676-78657695 | MS.gene73497:CDS | 40.0% | |
| TAAAAGCCTACAACCCTGTT+TGG | + | chr5.2:78657509-78657528 | None:intergenic | 40.0% | |
| TGAACCATGTTTGGCAAGTT+GGG | - | chr5.2:78657647-78657666 | MS.gene73497:CDS | 40.0% | |
| TGTTAGGGGTGATATACCTT+TGG | - | chr5.2:78657675-78657694 | MS.gene73497:CDS | 40.0% | |
| TTGAACCATGTTTGGCAAGT+TGG | - | chr5.2:78657646-78657665 | MS.gene73497:CDS | 40.0% | |
| ! | ATATTTGTTGGATGGCCCAA+AGG | + | chr5.2:78657694-78657713 | None:intergenic | 40.0% |
| AAGGAACCAAACAGGGTTGT+AGG | - | chr5.2:78657500-78657519 | MS.gene73497:CDS | 45.0% | |
| ATAGACTTGACTTCTCCCGA+TGG | - | chr5.2:78657739-78657758 | MS.gene73497:CDS | 45.0% | |
| ATAGCATGGGGTGTAAATCC+AGG | - | chr5.2:78657388-78657407 | MS.gene73497:CDS | 45.0% | |
| CCAATATGGATCAAGGGGAT+GGG | - | chr5.2:78657362-78657381 | MS.gene73497:CDS | 45.0% | |
| TAGCATGGGGTGTAAATCCA+GGG | - | chr5.2:78657389-78657408 | MS.gene73497:CDS | 45.0% | |
| TTATCGCTGCATGGTTGTTG+AGG | + | chr5.2:78657214-78657233 | None:intergenic | 45.0% | |
| !! | TCGGTGCCAATATGGATCAA+GGG | - | chr5.2:78657356-78657375 | MS.gene73497:CDS | 45.0% |
| !! | TTCGGTGCCAATATGGATCA+AGG | - | chr5.2:78657355-78657374 | MS.gene73497:CDS | 45.0% |
| AGCATGGGGTGTAAATCCAG+GGG | - | chr5.2:78657390-78657409 | MS.gene73497:CDS | 50.0% | |
| ATATGGATCAAGGGGATGGG+TGG | - | chr5.2:78657365-78657384 | MS.gene73497:CDS | 50.0% | |
| ATCATTTCAGCCCTTTCCCC+TGG | + | chr5.2:78657409-78657428 | None:intergenic | 50.0% | |
| CCCATCCCCTTGATCCATAT+TGG | + | chr5.2:78657365-78657384 | None:intergenic | 50.0% | |
| CCTTCCCAACTTGCCAAACA+TGG | + | chr5.2:78657654-78657673 | None:intergenic | 50.0% | |
| GCCAATATGGATCAAGGGGA+TGG | - | chr5.2:78657361-78657380 | MS.gene73497:CDS | 50.0% | |
| GCGGTAACTCCAACCGAATT+CGG | + | chr5.2:78657299-78657318 | None:intergenic | 50.0% | |
| GGGGAAAGGGCTGAAATGAT+TGG | - | chr5.2:78657409-78657428 | MS.gene73497:CDS | 50.0% | |
| GGGTGTAAATCCAGGGGAAA+GGG | - | chr5.2:78657396-78657415 | MS.gene73497:CDS | 50.0% | |
| TTCGCGAAGTTATCGCTGCA+TGG | + | chr5.2:78657223-78657242 | None:intergenic | 50.0% | |
| TTCGGTTGGAGTTACCGCAA+CGG | - | chr5.2:78657301-78657320 | MS.gene73497:CDS | 50.0% | |
| ! | CAAGTTGGGAAGGCTGTTAG+GGG | - | chr5.2:78657661-78657680 | MS.gene73497:CDS | 50.0% |
| ! | CCATGTTTGGCAAGTTGGGA+AGG | - | chr5.2:78657651-78657670 | MS.gene73497:CDS | 50.0% |
| ! | CGAACTCTAGGAGCCGAATT+CGG | - | chr5.2:78657283-78657302 | MS.gene73497:CDS | 50.0% |
| ! | CGGTGCCAATATGGATCAAG+GGG | - | chr5.2:78657357-78657376 | MS.gene73497:CDS | 50.0% |
| ! | GCAAGTTGGGAAGGCTGTTA+GGG | - | chr5.2:78657660-78657679 | MS.gene73497:CDS | 50.0% |
| ! | TGGTTGAGTTTGTGCCGTTG+CGG | + | chr5.2:78657318-78657337 | None:intergenic | 50.0% |
| AAGGGGATGGGTGGATAGCA+TGG | - | chr5.2:78657374-78657393 | MS.gene73497:CDS | 55.0% | |
| AGGGGATGGGTGGATAGCAT+GGG | - | chr5.2:78657375-78657394 | MS.gene73497:CDS | 55.0% | |
| GGGGTGTAAATCCAGGGGAA+AGG | - | chr5.2:78657395-78657414 | MS.gene73497:CDS | 55.0% | |
| ! | CTCTAGGAGCCGAATTCGGT+TGG | - | chr5.2:78657287-78657306 | MS.gene73497:CDS | 55.0% |
| ! | GGCAAGTTGGGAAGGCTGTT+AGG | - | chr5.2:78657659-78657678 | MS.gene73497:CDS | 55.0% |
| CTGTCCCTTGCTGCGACCAT+CGG | + | chr5.2:78657758-78657777 | None:intergenic | 60.0% | |
| GGGGATGGGTGGATAGCATG+GGG | - | chr5.2:78657376-78657395 | MS.gene73497:CDS | 60.0% | |
| TCTCCCGATGGTCGCAGCAA+GGG | - | chr5.2:78657751-78657770 | MS.gene73497:CDS | 60.0% | |
| TGTCCCTTGCTGCGACCATC+GGG | + | chr5.2:78657757-78657776 | None:intergenic | 60.0% | |
| TTCTCCCGATGGTCGCAGCA+AGG | - | chr5.2:78657750-78657769 | MS.gene73497:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 78657124 | 78657852 | 78657124 | ID=MS.gene73497 |
| chr5.2 | mRNA | 78657124 | 78657852 | 78657124 | ID=MS.gene73497.t1;Parent=MS.gene73497 |
| chr5.2 | exon | 78657124 | 78657852 | 78657124 | ID=MS.gene73497.t1.exon1;Parent=MS.gene73497.t1 |
| chr5.2 | CDS | 78657124 | 78657852 | 78657124 | ID=cds.MS.gene73497.t1;Parent=MS.gene73497.t1 |
| Gene Sequence |
| Protein sequence |
