Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73743.t1 | GAU39460.1 | 38 | 108 | 43 | 2 | 1 | 87 | 1 | 105 | 7.00E-10 | 73.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73743.t1 | A0A2Z6NRS6 | 38.0 | 108 | 43 | 2 | 1 | 87 | 1 | 105 | 5.0e-10 | 73.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050559 | MS.gene73743 | 0.803746 | 3.01E-49 | -1.69E-46 |
| MS.gene05548 | MS.gene73743 | 0.8149 | 1.22E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 31 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGATCAGTGTAAGGCAATT+TGG | 0.228687 | 7.3:+25235090 | MS.gene73743:CDS |
| GTCTACCAATGGAGAGGATT+TGG | 0.264471 | 7.3:+25235222 | MS.gene73743:CDS |
| TCTACCAATGGAGAGGATTT+GGG | 0.310714 | 7.3:+25235223 | MS.gene73743:CDS |
| GAAGCTTCATTGCTCATATC+TGG | 0.338136 | 7.3:-25235257 | None:intergenic |
| CAGTGGCTCATAGGACCTTC+TGG | 0.346451 | 7.3:-25235047 | None:intergenic |
| TGATCAGTGTAAGGCAATTT+GGG | 0.366956 | 7.3:+25235091 | MS.gene73743:CDS |
| GTACCTTACTTTGGAGTTTG+TGG | 0.381746 | 7.3:+25235199 | MS.gene73743:CDS |
| AACGAGTTAATCACCATTCC+TGG | 0.388834 | 7.3:-25235294 | None:intergenic |
| TCTATCTTGCAGTGGCTCAT+AGG | 0.404363 | 7.3:-25235056 | None:intergenic |
| TACCTTACTTTGGAGTTTGT+GGG | 0.423061 | 7.3:+25235200 | MS.gene73743:CDS |
| TCTTGAGGATGATCAGTGTA+AGG | 0.470969 | 7.3:+25235082 | MS.gene73743:CDS |
| CTTCTACCTCTCGAGTATCC+AGG | 0.495212 | 7.3:+25235276 | MS.gene73743:CDS |
| GGAGCTGAAAGGTGAGACTC+TGG | 0.501297 | 7.3:+25235112 | MS.gene73743:CDS |
| CCTCATTTGTAGTGCGAACA+AGG | 0.509994 | 7.3:-25235145 | None:intergenic |
| GAAGGTAGAGTACCTTACTT+TGG | 0.514894 | 7.3:+25235190 | MS.gene73743:CDS |
| GGAGTTTGTGGGTCTACCAA+TGG | 0.516668 | 7.3:+25235211 | MS.gene73743:CDS |
| GACCCACAAACTCCAAAGTA+AGG | 0.517101 | 7.3:-25235202 | None:intergenic |
| ATTTGTAGTGCGAACAAGGC+CGG | 0.534580 | 7.3:-25235141 | None:intergenic |
| GCTCCCCAAATCCTCTCCAT+TGG | 0.554872 | 7.3:-25235227 | None:intergenic |
| CCTTGTTCGCACTACAAATG+AGG | 0.557277 | 7.3:+25235145 | MS.gene73743:CDS |
| ACCTCTCGAGTATCCAGGAA+TGG | 0.576121 | 7.3:+25235281 | MS.gene73743:CDS |
| CTACCAATGGAGAGGATTTG+GGG | 0.599763 | 7.3:+25235224 | MS.gene73743:CDS |
| CGCACTACAAATGAGGTCGA+TGG | 0.605943 | 7.3:+25235152 | MS.gene73743:CDS |
| CTGCAAGATAGAGTTTCTTG+AGG | 0.626115 | 7.3:+25235067 | MS.gene73743:CDS |
| TTGTGGGTCTACCAATGGAG+AGG | 0.626640 | 7.3:+25235216 | MS.gene73743:CDS |
| TTCCTACAGATGTTGCCAGA+AGG | 0.629071 | 7.3:+25235032 | None:intergenic |
| AAGGCAATTTGGGAGCTGAA+AGG | 0.638835 | 7.3:+25235101 | MS.gene73743:CDS |
| CAAGAAACTCTATCTTGCAG+TGG | 0.642358 | 7.3:-25235064 | None:intergenic |
| ACCATTCCTGGATACTCGAG+AGG | 0.658945 | 7.3:-25235282 | None:intergenic |
| GGTGAGACTCTGGTTTACAC+CGG | 0.688295 | 7.3:+25235122 | MS.gene73743:CDS |
| TGGTTTGCTTGTGAGCACGA+AGG | 0.691002 | 7.3:+25235172 | MS.gene73743:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATGATCAGTGTAAGGCAATT+TGG | + | chr7.3:25235090-25235109 | MS.gene73743:CDS | 35.0% | |
| TGATCAGTGTAAGGCAATTT+GGG | + | chr7.3:25235091-25235110 | MS.gene73743:CDS | 35.0% | |
| !! | TACCTTACTTTGGAGTTTGT+GGG | + | chr7.3:25235200-25235219 | MS.gene73743:CDS | 35.0% |
| CAAGAAACTCTATCTTGCAG+TGG | - | chr7.3:25235067-25235086 | None:intergenic | 40.0% | |
| CTGCAAGATAGAGTTTCTTG+AGG | + | chr7.3:25235067-25235086 | MS.gene73743:CDS | 40.0% | |
| GAAGCTTCATTGCTCATATC+TGG | - | chr7.3:25235260-25235279 | None:intergenic | 40.0% | |
| TCTACCAATGGAGAGGATTT+GGG | + | chr7.3:25235223-25235242 | MS.gene73743:CDS | 40.0% | |
| TCTTGAGGATGATCAGTGTA+AGG | + | chr7.3:25235082-25235101 | MS.gene73743:CDS | 40.0% | |
| ! | GAAGGTAGAGTACCTTACTT+TGG | + | chr7.3:25235190-25235209 | MS.gene73743:CDS | 40.0% |
| !! | GTACCTTACTTTGGAGTTTG+TGG | + | chr7.3:25235199-25235218 | MS.gene73743:CDS | 40.0% |
| AAGGCAATTTGGGAGCTGAA+AGG | + | chr7.3:25235101-25235120 | MS.gene73743:CDS | 45.0% | |
| ATTTGTAGTGCGAACAAGGC+CGG | - | chr7.3:25235144-25235163 | None:intergenic | 45.0% | |
| CCTCATTTGTAGTGCGAACA+AGG | - | chr7.3:25235148-25235167 | None:intergenic | 45.0% | |
| CCTTGTTCGCACTACAAATG+AGG | + | chr7.3:25235145-25235164 | MS.gene73743:CDS | 45.0% | |
| CTACCAATGGAGAGGATTTG+GGG | + | chr7.3:25235224-25235243 | MS.gene73743:CDS | 45.0% | |
| GACCCACAAACTCCAAAGTA+AGG | - | chr7.3:25235205-25235224 | None:intergenic | 45.0% | |
| GTCTACCAATGGAGAGGATT+TGG | + | chr7.3:25235222-25235241 | MS.gene73743:CDS | 45.0% | |
| TCTATCTTGCAGTGGCTCAT+AGG | - | chr7.3:25235059-25235078 | None:intergenic | 45.0% | |
| ACCATTCCTGGATACTCGAG+AGG | - | chr7.3:25235285-25235304 | None:intergenic | 50.0% | |
| ACCTCTCGAGTATCCAGGAA+TGG | + | chr7.3:25235281-25235300 | MS.gene73743:CDS | 50.0% | |
| CGCACTACAAATGAGGTCGA+TGG | + | chr7.3:25235152-25235171 | MS.gene73743:CDS | 50.0% | |
| CTTCTACCTCTCGAGTATCC+AGG | + | chr7.3:25235276-25235295 | MS.gene73743:CDS | 50.0% | |
| GGAGTTTGTGGGTCTACCAA+TGG | + | chr7.3:25235211-25235230 | MS.gene73743:CDS | 50.0% | |
| TTGTGGGTCTACCAATGGAG+AGG | + | chr7.3:25235216-25235235 | MS.gene73743:CDS | 50.0% | |
| ! | GGTGAGACTCTGGTTTACAC+CGG | + | chr7.3:25235122-25235141 | MS.gene73743:CDS | 50.0% |
| !! | TGGTTTGCTTGTGAGCACGA+AGG | + | chr7.3:25235172-25235191 | MS.gene73743:CDS | 50.0% |
| CAGTGGCTCATAGGACCTTC+TGG | - | chr7.3:25235050-25235069 | None:intergenic | 55.0% | |
| GCTCCCCAAATCCTCTCCAT+TGG | - | chr7.3:25235230-25235249 | None:intergenic | 55.0% | |
| GGAGCTGAAAGGTGAGACTC+TGG | + | chr7.3:25235112-25235131 | MS.gene73743:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 25235041 | 25235310 | 25235041 | ID=MS.gene73743 |
| chr7.3 | mRNA | 25235041 | 25235310 | 25235041 | ID=MS.gene73743.t1;Parent=MS.gene73743 |
| chr7.3 | exon | 25235041 | 25235310 | 25235041 | ID=MS.gene73743.t1.exon1;Parent=MS.gene73743.t1 |
| chr7.3 | CDS | 25235041 | 25235310 | 25235041 | ID=cds.MS.gene73743.t1;Parent=MS.gene73743.t1 |
| Gene Sequence |
| Protein sequence |
