Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73772.t1 | XP_003624346.2 | 86.9 | 191 | 19 | 3 | 1 | 189 | 46 | 232 | 5.00E-50 | 207.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73772.t1 | G7KUB4 | 86.9 | 191 | 19 | 3 | 1 | 189 | 1 | 187 | 3.6e-50 | 207.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050297 | MS.gene73772 | 0.803712 | 3.06E-49 | -1.69E-46 |
| MS.gene052367 | MS.gene73772 | 0.808698 | 2.72E-50 | -1.69E-46 |
| MS.gene060473 | MS.gene73772 | 0.803016 | 4.26E-49 | -1.69E-46 |
| MS.gene061260 | MS.gene73772 | 0.808209 | 3.47E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73772.t1 | MTR_7g082230 | 93.194 | 191 | 7 | 3 | 1 | 189 | 1 | 187 | 7.28e-118 | 332 |
| MS.gene73772.t1 | MTR_6g018780 | 50.336 | 149 | 64 | 4 | 36 | 178 | 302 | 446 | 1.38e-31 | 119 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73772.t1 | AT1G67035 | 41.420 | 169 | 84 | 7 | 3 | 167 | 38 | 195 | 3.48e-24 | 95.1 |
| MS.gene73772.t1 | AT5G38300 | 38.191 | 199 | 82 | 9 | 3 | 167 | 43 | 234 | 1.74e-19 | 83.6 |
| MS.gene73772.t1 | AT1G67035 | 39.844 | 128 | 67 | 5 | 3 | 126 | 38 | 159 | 7.75e-15 | 69.7 |
Find 31 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATGTTAGTAGTGTTGCTTC+TGG | 0.196267 | 7.3:+25542864 | MS.gene73772:CDS |
| CGTAACATTGAAGAGAAATC+TGG | 0.289550 | 7.3:-25542927 | None:intergenic |
| GTTGTTGTTGTTGTAGTCTT+AGG | 0.312780 | 7.3:-25542761 | None:intergenic |
| CCACAACAACACCATGGATA+TGG | 0.328554 | 7.3:+25542677 | None:intergenic |
| ATGTTAGTAGTGTTGCTTCT+GGG | 0.377348 | 7.3:+25542865 | MS.gene73772:CDS |
| AGTAGGAATAGTAGAGTTGT+GGG | 0.384607 | 7.3:+25543197 | MS.gene73772:CDS |
| AGTAGTGTTGCTTCTGGGAC+TGG | 0.398203 | 7.3:+25542870 | MS.gene73772:CDS |
| CTAGGTTGTGGTATATTTGT+CGG | 0.427141 | 7.3:-25542966 | None:intergenic |
| AAAGGGTGGTGGGATTCTTA+TGG | 0.429771 | 7.3:+25543100 | MS.gene73772:CDS |
| TTAGCGTATGCGTTGTATCT+TGG | 0.436580 | 7.3:-25542708 | None:intergenic |
| GCTTCTGGGACTGGAAGAAA+AGG | 0.447686 | 7.3:+25542879 | MS.gene73772:CDS |
| GAGTAGGAATAGTAGAGTTG+TGG | 0.459261 | 7.3:+25543196 | MS.gene73772:CDS |
| CCTTTGTTCTTGTTCTTCAA+AGG | 0.465291 | 7.3:-25543008 | None:intergenic |
| TTGTTCTTGTTCTTCAAAGG+AGG | 0.514167 | 7.3:-25543005 | None:intergenic |
| TGGTCCTCTCTCCATATCCA+TGG | 0.530913 | 7.3:-25542688 | None:intergenic |
| GCTACTGCAAATGCTATCAA+TGG | 0.535004 | 7.3:+25543170 | MS.gene73772:CDS |
| ATGCTATCAATGGTGAGAGT+AGG | 0.543176 | 7.3:+25543180 | MS.gene73772:CDS |
| TGCTGCTGCAGAGAAGAGAA+AGG | 0.551914 | 7.3:+25543082 | MS.gene73772:CDS |
| CAGAGAAGAGAAAGGGTGGT+GGG | 0.587887 | 7.3:+25543090 | MS.gene73772:CDS |
| AACAAGAACAAAGGTTGTAG+TGG | 0.592930 | 7.3:+25543017 | MS.gene73772:CDS |
| GCAGAGAAGAGAAAGGGTGG+TGG | 0.593952 | 7.3:+25543089 | MS.gene73772:CDS |
| GGTGTCAAAGTAGTAGTACT+AGG | 0.601055 | 7.3:-25542984 | None:intergenic |
| AAAGTAGTAGTACTAGGTTG+TGG | 0.616412 | 7.3:-25542978 | None:intergenic |
| GCTGCTGCAGAGAAGAGAAA+GGG | 0.619325 | 7.3:+25543083 | MS.gene73772:CDS |
| CTTCTGGGACTGGAAGAAAA+GGG | 0.620822 | 7.3:+25542880 | MS.gene73772:CDS |
| GTAGGAATAGTAGAGTTGTG+GGG | 0.633060 | 7.3:+25543198 | MS.gene73772:CDS |
| AAGAACAAAGGTTGTAGTGG+TGG | 0.637569 | 7.3:+25543020 | MS.gene73772:CDS |
| GTGGTGGGATTCTTATGGCA+AGG | 0.650830 | 7.3:+25543105 | MS.gene73772:CDS |
| AACACCATGGATATGGAGAG+AGG | 0.667075 | 7.3:+25542684 | None:intergenic |
| CCTTTGAAGAACAAGAACAA+AGG | 0.667234 | 7.3:+25543008 | MS.gene73772:CDS |
| GCTGCAGAGAAGAGAAAGGG+TGG | 0.731860 | 7.3:+25543086 | MS.gene73772:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGAAACTTGACTTGTTTTGT+TGG | - | chr7.3:25542846-25542865 | None:intergenic | 30.0% |
| AAAGTAGTAGTACTAGGTTG+TGG | - | chr7.3:25542981-25543000 | None:intergenic | 35.0% | |
| AACAAGAACAAAGGTTGTAG+TGG | + | chr7.3:25543017-25543036 | MS.gene73772:CDS | 35.0% | |
| AGTAGGAATAGTAGAGTTGT+GGG | + | chr7.3:25543197-25543216 | MS.gene73772:CDS | 35.0% | |
| CCTTTGTTCTTGTTCTTCAA+AGG | - | chr7.3:25543011-25543030 | None:intergenic | 35.0% | |
| CGTAACATTGAAGAGAAATC+TGG | - | chr7.3:25542930-25542949 | None:intergenic | 35.0% | |
| CTAGGTTGTGGTATATTTGT+CGG | - | chr7.3:25542969-25542988 | None:intergenic | 35.0% | |
| TTGTTCTTGTTCTTCAAAGG+AGG | - | chr7.3:25543008-25543027 | None:intergenic | 35.0% | |
| ! | CCTTTGAAGAACAAGAACAA+AGG | + | chr7.3:25543008-25543027 | MS.gene73772:CDS | 35.0% |
| ! | GTGTTTTGTCAAGTTCAAGA+GGG | + | chr7.3:25543042-25543061 | MS.gene73772:CDS | 35.0% |
| ! | GTTGTTGTTGTTGTAGTCTT+AGG | - | chr7.3:25542764-25542783 | None:intergenic | 35.0% |
| !! | ATGTTAGTAGTGTTGCTTCT+GGG | + | chr7.3:25542865-25542884 | MS.gene73772:CDS | 35.0% |
| !!! | AACTTGACTTGTTTTGTTGG+TGG | - | chr7.3:25542843-25542862 | None:intergenic | 35.0% |
| AAGAACAAAGGTTGTAGTGG+TGG | + | chr7.3:25543020-25543039 | MS.gene73772:CDS | 40.0% | |
| ATGCTATCAATGGTGAGAGT+AGG | + | chr7.3:25543180-25543199 | MS.gene73772:CDS | 40.0% | |
| GAGTAGGAATAGTAGAGTTG+TGG | + | chr7.3:25543196-25543215 | MS.gene73772:CDS | 40.0% | |
| GCTACTGCAAATGCTATCAA+TGG | + | chr7.3:25543170-25543189 | MS.gene73772:CDS | 40.0% | |
| GGTGTCAAAGTAGTAGTACT+AGG | - | chr7.3:25542987-25543006 | None:intergenic | 40.0% | |
| GTAGGAATAGTAGAGTTGTG+GGG | + | chr7.3:25543198-25543217 | MS.gene73772:CDS | 40.0% | |
| TTAGCGTATGCGTTGTATCT+TGG | - | chr7.3:25542711-25542730 | None:intergenic | 40.0% | |
| ! | GGTGTTTTGTCAAGTTCAAG+AGG | + | chr7.3:25543041-25543060 | MS.gene73772:CDS | 40.0% |
| !! | CATGTTAGTAGTGTTGCTTC+TGG | + | chr7.3:25542864-25542883 | MS.gene73772:CDS | 40.0% |
| !!! | ACTTGTTTTGTTGGTGGAGT+TGG | - | chr7.3:25542837-25542856 | None:intergenic | 40.0% |
| !! | AAAGGGTGGTGGGATTCTTA+TGG | + | chr7.3:25543100-25543119 | MS.gene73772:CDS | 45.0% |
| !! | CTTCTGGGACTGGAAGAAAA+GGG | + | chr7.3:25542880-25542899 | MS.gene73772:CDS | 45.0% |
| CAGAGAAGAGAAAGGGTGGT+GGG | + | chr7.3:25543090-25543109 | MS.gene73772:CDS | 50.0% | |
| GCTGCTGCAGAGAAGAGAAA+GGG | + | chr7.3:25543083-25543102 | MS.gene73772:CDS | 50.0% | |
| TGCTGCTGCAGAGAAGAGAA+AGG | + | chr7.3:25543082-25543101 | MS.gene73772:CDS | 50.0% | |
| ! | GTGGTGGGATTCTTATGGCA+AGG | + | chr7.3:25543105-25543124 | MS.gene73772:CDS | 50.0% |
| !! | AGTAGTGTTGCTTCTGGGAC+TGG | + | chr7.3:25542870-25542889 | MS.gene73772:CDS | 50.0% |
| !! | GCTTCTGGGACTGGAAGAAA+AGG | + | chr7.3:25542879-25542898 | MS.gene73772:CDS | 50.0% |
| GCAGAGAAGAGAAAGGGTGG+TGG | + | chr7.3:25543089-25543108 | MS.gene73772:CDS | 55.0% | |
| GCTGCAGAGAAGAGAAAGGG+TGG | + | chr7.3:25543086-25543105 | MS.gene73772:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 25542690 | 25543259 | 25542690 | ID=MS.gene73772 |
| chr7.3 | mRNA | 25542690 | 25543259 | 25542690 | ID=MS.gene73772.t1;Parent=MS.gene73772 |
| chr7.3 | exon | 25542690 | 25543259 | 25542690 | ID=MS.gene73772.t1.exon1;Parent=MS.gene73772.t1 |
| chr7.3 | CDS | 25542690 | 25543259 | 25542690 | ID=cds.MS.gene73772.t1;Parent=MS.gene73772.t1 |
| Gene Sequence |
| Protein sequence |
