Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060473.t1 | XP_013458063.1 | 89.3 | 672 | 66 | 2 | 1 | 672 | 1 | 666 | 0.00E+00 | 1092.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| No data available in table | |||||||||||
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060473.t1 | A0A072UQI4 | 89.3 | 672 | 66 | 2 | 1 | 672 | 1 | 666 | 0.0e+00 | 1092.4 |
TFs/TRs:
Protein Kinases:
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049397 | MS.gene060473 | 0.845882 | 3.08E-59 | -1.69E-46 |
| MS.gene049596 | MS.gene060473 | 0.805424 | 1.34E-49 | -1.69E-46 |
| MS.gene049622 | MS.gene060473 | 0.836227 | 1.06E-56 | -1.69E-46 |
| MS.gene049660 | MS.gene060473 | 0.830348 | 3.09E-55 | -1.69E-46 |
| MS.gene04985 | MS.gene060473 | 0.810934 | 9.00E-51 | -1.69E-46 |
| MS.gene049989 | MS.gene060473 | 0.905388 | 4.52E-80 | -1.69E-46 |
| MS.gene050297 | MS.gene060473 | 0.884641 | 1.61E-71 | -1.69E-46 |
| MS.gene05054 | MS.gene060473 | 0.832805 | 7.66E-56 | -1.69E-46 |
| MS.gene050648 | MS.gene060473 | 0.80176 | 7.73E-49 | -1.69E-46 |
| MS.gene051094 | MS.gene060473 | 0.82749 | 1.52E-54 | -1.69E-46 |
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060473.t1 | MTR_4g112035 | 94.940 | 672 | 28 | 3 | 1 | 672 | 1 | 666 | 0.0 | 1212 |
| MS.gene060473.t1 | MTR_0050s0010 | 58.824 | 119 | 49 | 0 | 1 | 119 | 1 | 119 | 1.03e-41 | 159 |
| MS.gene060473.t1 | MTR_0050s0010 | 28.772 | 285 | 138 | 9 | 415 | 667 | 282 | 533 | 2.42e-22 | 101 |
| MS.gene060473.t1 | MTR_4g068050 | 55.000 | 120 | 54 | 0 | 1 | 120 | 1 | 120 | 2.19e-39 | 155 |
| MS.gene060473.t1 | MTR_4g068050 | 25.934 | 482 | 222 | 18 | 296 | 661 | 245 | 707 | 6.68e-20 | 94.7 |
| MS.gene060473.t1 | MTR_5g034370 | 48.760 | 121 | 61 | 1 | 1 | 120 | 1 | 121 | 6.59e-33 | 134 |
| MS.gene060473.t1 | MTR_2g062300 | 45.082 | 122 | 61 | 2 | 1 | 120 | 1 | 118 | 2.69e-21 | 99.0 |
| MS.gene060473.t1 | MTR_2g099095 | 26.509 | 464 | 221 | 16 | 302 | 661 | 271 | 718 | 4.37e-15 | 79.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060473.t1 | AT4G13370 | 52.849 | 702 | 267 | 20 | 1 | 668 | 1 | 672 | 0.0 | 621 |
| MS.gene060473.t1 | AT3G14170 | 53.600 | 125 | 58 | 0 | 1 | 125 | 1 | 125 | 3.30e-39 | 152 |
| MS.gene060473.t1 | AT3G14170 | 33.058 | 242 | 145 | 8 | 425 | 661 | 270 | 499 | 7.89e-21 | 97.1 |
| MS.gene060473.t1 | AT3G14170 | 53.600 | 125 | 58 | 0 | 1 | 125 | 1 | 125 | 3.31e-39 | 152 |
| MS.gene060473.t1 | AT3G14170 | 33.058 | 242 | 145 | 8 | 425 | 661 | 269 | 498 | 8.21e-21 | 97.1 |
| MS.gene060473.t1 | AT1G08760 | 54.167 | 120 | 55 | 0 | 1 | 120 | 1 | 120 | 9.38e-38 | 150 |
| MS.gene060473.t1 | AT1G23790 | 48.760 | 121 | 61 | 1 | 1 | 120 | 1 | 121 | 3.00e-31 | 129 |
| MS.gene060473.t1 | AT1G23790 | 25.442 | 283 | 177 | 6 | 335 | 609 | 220 | 476 | 9.30e-12 | 68.6 |
| MS.gene060473.t1 | AT1G70340 | 46.667 | 120 | 63 | 1 | 1 | 119 | 1 | 120 | 1.10e-28 | 120 |
| MS.gene060473.t1 | AT1G70340 | 27.320 | 194 | 115 | 5 | 421 | 595 | 266 | 452 | 5.80e-11 | 65.9 |
Find 178 sgRNAs with CRISPR-Local
Find 223 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATAATTCAGGATGAAGC+AGG | 0.470051 | 8.2:+15438306 | None:intergenic |
| AAAGCATTGCCTCTGGATGG+TGG | 0.600362 | 8.2:-15438495 | MS.gene060473:CDS |
| AAATAATTCAGGATGAAGCA+GGG | 0.580487 | 8.2:+15438307 | None:intergenic |
| AAATCCAAGTTTGCAGGTCT+TGG | 0.376268 | 8.2:-15439614 | MS.gene060473:CDS |
| AAATGCATGGTTGGACCGCG+TGG | 0.636414 | 8.2:-15438436 | MS.gene060473:CDS |
| AAATGGAATGAAAGAAACAC+TGG | 0.581658 | 8.2:-15438628 | MS.gene060473:CDS |
| AAATTATCTCGTGGAGCAAG+TGG | 0.614804 | 8.2:+15440449 | None:intergenic |
| AACACCGACACACGTGTCAC+CGG | 0.513735 | 8.2:-15440929 | MS.gene060473:CDS |
| AACCTAAAGAAAAGAGTGTT+GGG | 0.495676 | 8.2:-15439977 | MS.gene060473:CDS |
| AACTTGTCTATGGTTGGCAA+AGG | 0.553870 | 8.2:+15438888 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAATGGAATGAAAGAAACAC+TGG | - | chr8.2:15440663-15440682 | MS.gene060473:CDS | 30.0% | |
| ACGATTATTGAGAATGAGAT+CGG | + | chr8.2:15438528-15438547 | None:intergenic | 30.0% | |
| AGATGAACAATGTTCATTCA+CGG | - | chr8.2:15438816-15438835 | MS.gene060473:CDS | 30.0% | |
| ATACAAAGAAAGAGGATCAT+CGG | + | chr8.2:15438726-15438745 | None:intergenic | 30.0% | |
| ATGATAGGTTTGTAGATGAT+AGG | - | chr8.2:15439928-15439947 | MS.gene060473:intron | 30.0% | |
| ATGTGACAAAACAATAGCAA+TGG | + | chr8.2:15440824-15440843 | None:intergenic | 30.0% | |
| CCAAAATAATGGTCAATTCA+AGG | + | chr8.2:15439972-15439991 | None:intergenic | 30.0% | |
| CTCAATAATCGTCTTCAATT+AGG | - | chr8.2:15438536-15438555 | MS.gene060473:CDS | 30.0% | |
| CTTGTTTACTTCCTCTTTAA+TGG | - | chr8.2:15440292-15440311 | MS.gene060473:CDS | 30.0% | |
| GATGGTAAAAAACTTGTCTA+TGG | + | chr8.2:15440416-15440435 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 15438314 | 15440999 | 15438314 | ID=MS.gene060473 |
| chr8.2 | mRNA | 15438314 | 15440999 | 15438314 | ID=MS.gene060473.t1;Parent=MS.gene060473 |
| chr8.2 | exon | 15439935 | 15440999 | 15439935 | ID=MS.gene060473.t1.exon1;Parent=MS.gene060473.t1 |
| chr8.2 | CDS | 15439935 | 15440999 | 15439935 | ID=cds.MS.gene060473.t1;Parent=MS.gene060473.t1 |
| chr8.2 | exon | 15439529 | 15439753 | 15439529 | ID=MS.gene060473.t1.exon2;Parent=MS.gene060473.t1 |
| chr8.2 | CDS | 15439529 | 15439753 | 15439529 | ID=cds.MS.gene060473.t1;Parent=MS.gene060473.t1 |
| chr8.2 | exon | 15439163 | 15439254 | 15439163 | ID=MS.gene060473.t1.exon3;Parent=MS.gene060473.t1 |
| chr8.2 | CDS | 15439163 | 15439254 | 15439163 | ID=cds.MS.gene060473.t1;Parent=MS.gene060473.t1 |
| chr8.2 | exon | 15438314 | 15438950 | 15438314 | ID=MS.gene060473.t1.exon4;Parent=MS.gene060473.t1 |
| chr8.2 | CDS | 15438314 | 15438950 | 15438314 | ID=cds.MS.gene060473.t1;Parent=MS.gene060473.t1 |
| Gene Sequence |
>MS.gene060473 ATGGCTTCACTAACACCAGGCCTAATCCTTAAACTCCTACAAGCAATGAACACCGACACACGTGTCACCGGCGACCACCGTTCTCCTCTTCTCCAACTCATCGGAATTGTACCCGCTTTATCCAGCTCCGATCTCTTCTCCAACGAAGGCTTCTTTCTCAACCTCTCTGATTCTCTCAACTCCACCTACGTTCTTCTCTCTCACCCTGACACCGATCTCATTCTCAATAATCGTCTTCAATTAGGCCAGTTCCTCTACGTTGATCGCTTTCATTTCCACACCCCTCTTCCTTCTGTCACCAACATTCGTCCTCTCCCCACGCGCCACCCCTTCGTCGGAACGCCGGAACCACTCGTAGCTCGTATTTCACCTACCACTCGTCATTTCACCATTCAACCATTATCAGATTCCGATGATCCTCTTTCTTTGTATCTCTCCACCAACACAACCACCGCACAATCTCCCATTCAGATCAATCAACAACAACAACAGCAGCAGAAGAAGATGAACAATGTTCATTCACGGCAACCACTTGCTCCACGAGATAATTTACCAACGCCGAAGAGATTTTCATCTCCGGCGACTGCTAAACGATCACAATCTGCAGGGAAGTTTGGTAAAGTTTCTGCTGAGAGAGATCCATCTCCGGCGGGGAAGATGAAGAGATCTTCTTCACCAGTACCGTCCAAATGTGTGGTTCCGAGTTTGGTTTCTGCTCGAGAGGACAATCGGAAGGTTTCACGGGAAGCCGCAATTATTGTTCCGTCGAGGTATAGACAGCCGTCTCCGACGACGGCAAGGAAGCAGCCTTCGCCGAATCCTAGAAGAGCTTCCATTTCTCCGGGGAGAAGATTGTCCGGTGGAATCAAGTTTTCGCCGGCTGTTGGAGATTCTACTGCGAAGAAGAAGATGGTAGCTGGGATTTCTAAGATTTCTGATATGTTGGCTGGATCGGCGAAGACCGCGAGGAAGAATTGGGATGAACAAAATGTTGAAGGTGAACCTAAAGAAAAGAGTGTTGGGTCTAAAACTAGGGTTGATTCTCAATCAATTTTGAGAACTCAGGTAAATAAACATCAAATTTAAATGCTATTTTTTTTTTTTTTTTATATAGGGCATGAATTAATTAATGAATGCTTAATTGGAAATTCAAATCAGAAATTATGAATTTAGATTGAGCTAAGAATGGAAAGTTTCAGCATATTTAGTGTGATTTGTATTGAAATGATGCTGATTTTTTTGGCAGGTTTCAATGACAAGGAGATTGAGTGATGTGAAAAGCCGAAAATCAGATGACAATGATTCTTCTTCTATAGATGAGAATACTATAGACTCTTCTCCTCAAAGTTGTTTGGAGGTAGAGAAATCCAAGTTTGCAGGTCTTGGTGGCATTACAATTCATGAAAAGAAATGGACTGATGGAAGTGTTCCTCTTGATGCTGTGTCTGGCAAACTTTCAAGGCTTGGAAAGGTATTAATTTTGGAAATTTAACATTCATTTCTAGTGGTCTTATAATATGGAAATCATGCATAAGTATATAAACAGATTGTGCCACCACTAAAAAATGCAGTGAATACAAAAATGCAGTATGATTGAGTGTTTATGCATTAGAAATGATAGGTTTGTAGATGATAGGCTGGCTGGTTTCTTATAACCTTGAATTGACCATTATTTTGGTGATTTGGCTCTATTTGATTAGAAGGCAAATTTGATTAAATTGGTTTTTATTGTGAACTGAATCCAGGATGCAATGGAAAGGAAAGCTCTTGCTTCTGCAGCTGCTGCTGCAGCATTAGAAGAGGCCAATGCTACTGAATGTATAATAAGAAATTTAAGGTAACGATTTCATTTGACCTGGTTTTTAACATTGGAACTGAGTCTTTTATATACCAAAGTTAAGTCCATTGGCATTGTTTTCATTTTATTTAAACTTCAACTGAATGATCCATTTACTATTCAAGGGTTTAAAATTCATAACTTGTTTACTTCCTCTTTAATGGCTGACATGTATTTTACTTATTTTTTAATGTTCTTTCTGCCTGCTTCAGCATGTTTTCAGACCTTTGCTCTGTGTGTAAAGCCAAAAATCCTTTGCCAACCATAGACAAGTTTTTTACCATCTATGATGATGTCCTAAGATCAATCGCACTGGCTGAATCTGTTGCTAATAGTCACGATAACAGTATTCCAACATGTCAATCAAAGTCCATCTCTCTTTGGGTTGAAGCTGCTTTGGCCACTGATCTTCAGATAGTATCCCTTCTTACTGAAAATGACATAGATACTCCATCAGAACTTCAGAAAAGGCTGTCAAGACCACACTCTCTTAGCACATCTAAGAGTCAAGGAAATGGAATGAAAGAAACACTGGAGCTTGGAACAAGTTTACTGTCAGAAATGCAAATGTGGTTTCTACGCTTTGTTGAAGAATCCCTTGAAGCAGGATTTAAAGTGTTTGGAGAGAGTGCTAGTGGTGGGAAGAAAGCATTGCCTCTGGATGGTGGCTCCATTGCTATTGTTTTGTCACATTTGAAGCGAGTAAATGCATGGTTGGACCGCGTGGTTTCTAAAGGGAATCACTCATTGACAGATAAGATTGAGAAATTAAAGAGAAAAATCTATGGTTTTGTTATCCAGCATGTAGGATCAACTTTTGATAACAAAGCTTCCCCTGCTTCATCCTGA |
| Protein sequence |
>MS.gene060473.t1 MASLTPGLILKLLQAMNTDTRVTGDHRSPLLQLIGIVPALSSSDLFSNEGFFLNLSDSLNSTYVLLSHPDTDLILNNRLQLGQFLYVDRFHFHTPLPSVTNIRPLPTRHPFVGTPEPLVARISPTTRHFTIQPLSDSDDPLSLYLSTNTTTAQSPIQINQQQQQQQKKMNNVHSRQPLAPRDNLPTPKRFSSPATAKRSQSAGKFGKVSAERDPSPAGKMKRSSSPVPSKCVVPSLVSAREDNRKVSREAAIIVPSRYRQPSPTTARKQPSPNPRRASISPGRRLSGGIKFSPAVGDSTAKKKMVAGISKISDMLAGSAKTARKNWDEQNVEGEPKEKSVGSKTRVDSQSILRTQVSMTRRLSDVKSRKSDDNDSSSIDENTIDSSPQSCLEVEKSKFAGLGGITIHEKKWTDGSVPLDAVSGKLSRLGKDAMERKALASAAAAAALEEANATECIIRNLSMFSDLCSVCKAKNPLPTIDKFFTIYDDVLRSIALAESVANSHDNSIPTCQSKSISLWVEAALATDLQIVSLLTENDIDTPSELQKRLSRPHSLSTSKSQGNGMKETLELGTSLLSEMQMWFLRFVEESLEAGFKVFGESASGGKKALPLDGGSIAIVLSHLKRVNAWLDRVVSKGNHSLTDKIEKLKRKIYGFVIQHVGSTFDNKASPASS |
