Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74797.t1 | AFK36374.1 | 97.8 | 46 | 1 | 0 | 1 | 46 | 1 | 46 | 6.20E-17 | 95.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74797.t1 | Q9FZ62 | 62.8 | 43 | 16 | 0 | 4 | 46 | 7 | 49 | 1.4e-09 | 62.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74797.t1 | B7FI56 | 97.8 | 46 | 1 | 0 | 1 | 46 | 1 | 46 | 4.5e-17 | 95.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053356 | MS.gene74797 | 0.802036 | 6.79E-49 | -1.69E-46 |
| MS.gene054093 | MS.gene74797 | 0.823259 | 1.52E-53 | -1.69E-46 |
| MS.gene057835 | MS.gene74797 | 0.814226 | 1.72E-51 | -1.69E-46 |
| MS.gene058426 | MS.gene74797 | 0.807368 | 5.23E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74797.t1 | MTR_2g100340 | 97.826 | 46 | 1 | 0 | 1 | 46 | 1 | 46 | 4.40e-28 | 100 |
| MS.gene74797.t1 | MTR_8g015910 | 67.442 | 43 | 14 | 0 | 4 | 46 | 5 | 47 | 8.07e-16 | 68.6 |
| MS.gene74797.t1 | MTR_2g100350 | 63.043 | 46 | 17 | 0 | 1 | 46 | 1 | 46 | 2.41e-15 | 67.4 |
| MS.gene74797.t1 | MTR_8g015950 | 62.791 | 43 | 16 | 0 | 4 | 46 | 5 | 47 | 4.49e-15 | 66.6 |
| MS.gene74797.t1 | MTR_8g015960 | 60.465 | 43 | 17 | 0 | 4 | 46 | 5 | 47 | 1.03e-13 | 62.8 |
| MS.gene74797.t1 | MTR_2g062320 | 63.043 | 46 | 17 | 0 | 1 | 46 | 1 | 46 | 1.49e-13 | 62.4 |
| MS.gene74797.t1 | MTR_8g015940 | 62.791 | 43 | 16 | 0 | 4 | 46 | 5 | 47 | 2.60e-13 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74797.t1 | AT1G73010 | 65.116 | 43 | 15 | 0 | 4 | 46 | 14 | 56 | 9.60e-15 | 65.9 |
| MS.gene74797.t1 | AT1G17710 | 62.791 | 43 | 16 | 0 | 4 | 46 | 7 | 49 | 3.92e-13 | 61.2 |
| MS.gene74797.t1 | AT4G29530 | 58.696 | 46 | 19 | 0 | 1 | 46 | 1 | 46 | 1.54e-12 | 59.3 |
Find 7 sgRNAs with CRISPR-Local
Find 7 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGAATGTGATAGTGATAAT+TGG | 0.238396 | 7.1:-85606724 | MS.gene74797:CDS |
| AATTGGGTTCTTGATGAGTT+TGG | 0.346908 | 7.1:-85606707 | MS.gene74797:CDS |
| AGCTTCTTCGTAGCATGCTT+TGG | 0.347446 | 7.1:-85606664 | MS.gene74797:CDS |
| AAGAAGCTGATAGAATTTCT+CGG | 0.478833 | 7.1:+85606679 | None:intergenic |
| TGAATGTGATAGTGATAATT+GGG | 0.488124 | 7.1:-85606723 | MS.gene74797:CDS |
| CATGCTTTGGAATCCTCTCA+TGG | 0.547902 | 7.1:-85606651 | MS.gene74797:CDS |
| AGTGTAAAGAAGACCATGAG+AGG | 0.647030 | 7.1:+85606638 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGAATGTGATAGTGATAATT+GGG | - | chr7.1:85606666-85606685 | MS.gene74797:CDS | 25.0% |
| !! | TTGAATGTGATAGTGATAAT+TGG | - | chr7.1:85606665-85606684 | MS.gene74797:CDS | 25.0% |
| AAGAAGCTGATAGAATTTCT+CGG | + | chr7.1:85606713-85606732 | None:intergenic | 30.0% | |
| AATTGGGTTCTTGATGAGTT+TGG | - | chr7.1:85606682-85606701 | MS.gene74797:CDS | 35.0% | |
| AGTGTAAAGAAGACCATGAG+AGG | + | chr7.1:85606754-85606773 | None:intergenic | 40.0% | |
| AGCTTCTTCGTAGCATGCTT+TGG | - | chr7.1:85606725-85606744 | MS.gene74797:CDS | 45.0% | |
| !! | CATGCTTTGGAATCCTCTCA+TGG | - | chr7.1:85606738-85606757 | MS.gene74797:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 85606622 | 85606789 | 85606622 | ID=MS.gene74797 |
| chr7.1 | mRNA | 85606622 | 85606789 | 85606622 | ID=MS.gene74797.t1;Parent=MS.gene74797 |
| chr7.1 | exon | 85606622 | 85606789 | 85606622 | ID=MS.gene74797.t1.exon1;Parent=MS.gene74797.t1 |
| chr7.1 | CDS | 85606622 | 85606789 | 85606622 | ID=cds.MS.gene74797.t1;Parent=MS.gene74797.t1 |
| Gene Sequence |
| Protein sequence |
