Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74850.t1 | XP_013468922.1 | 98.7 | 379 | 3 | 1 | 1 | 377 | 1 | 379 | 1.80E-208 | 734.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74850.t1 | Q9ZT87 | 78.4 | 379 | 79 | 2 | 1 | 377 | 1 | 378 | 5.9e-171 | 601.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74850.t1 | A0A072VLU0 | 98.7 | 379 | 3 | 1 | 1 | 377 | 1 | 379 | 1.3e-208 | 734.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene74850 | MS.gene005004 | PPI |
| MS.gene07548 | MS.gene74850 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74850.t1 | MTR_1g078400 | 98.681 | 379 | 3 | 1 | 1 | 377 | 1 | 379 | 0.0 | 767 |
| MS.gene74850.t1 | MTR_1g078400 | 98.438 | 320 | 3 | 1 | 1 | 318 | 1 | 320 | 0.0 | 647 |
| MS.gene74850.t1 | MTR_7g102760 | 43.367 | 392 | 203 | 6 | 1 | 376 | 1 | 389 | 3.15e-98 | 298 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74850.t1 | AT4G27650 | 78.364 | 379 | 79 | 2 | 1 | 377 | 1 | 378 | 0.0 | 625 |
| MS.gene74850.t1 | AT4G27650 | 78.364 | 379 | 79 | 2 | 1 | 377 | 1 | 378 | 0.0 | 625 |
| MS.gene74850.t1 | AT4G27650 | 78.364 | 379 | 79 | 2 | 1 | 377 | 1 | 378 | 0.0 | 625 |
| MS.gene74850.t1 | AT3G58390 | 70.000 | 400 | 92 | 4 | 1 | 377 | 1 | 395 | 0.0 | 569 |
Find 70 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCAATTCAGTTAAGGATTC+TGG | 0.172762 | 1.1:-58781665 | MS.gene74850:CDS |
| GCATAGTTTAAAAGAGGTTT+TGG | 0.278574 | 1.1:-58783863 | MS.gene74850:CDS |
| TTAGCCCATATCCTCCTTAT+TGG | 0.278692 | 1.1:-58786040 | MS.gene74850:CDS |
| ATATCCTCCTTATTGGTAAA+AGG | 0.288893 | 1.1:-58786033 | MS.gene74850:intron |
| TTTATCATGTTCATGACATT+TGG | 0.301926 | 1.1:+58783835 | None:intergenic |
| GTAACTCAAGTTCTAAAGTA+TGG | 0.306803 | 1.1:+58786670 | None:intergenic |
| TTTGCCTTTCGTAGGATGTC+TGG | 0.312960 | 1.1:-58786437 | MS.gene74850:intron |
| AATTGAACCTTAACGCTTCC+AGG | 0.331018 | 1.1:+58787747 | None:intergenic |
| TGAACGTGTTAAACTCAAAT+TGG | 0.335052 | 1.1:-58787531 | MS.gene74850:CDS |
| TGTCTGGGATTCTTATGCTT+TGG | 0.353497 | 1.1:-58786421 | MS.gene74850:CDS |
| GATTCATCACGTGCTTGTTA+TGG | 0.355531 | 1.1:-58782364 | MS.gene74850:CDS |
| TTTGCACAGCTAGGCGTTCA+TGG | 0.362499 | 1.1:+58782320 | None:intergenic |
| CAATCATGGCCGTTACTATC+AGG | 0.364180 | 1.1:-58787592 | MS.gene74850:CDS |
| ATCTCCATGTCTTCGAGATC+AGG | 0.375283 | 1.1:+58781297 | None:intergenic |
| CTGGGATTCTTATGCTTTGG+AGG | 0.376577 | 1.1:-58786418 | MS.gene74850:CDS |
| TAACTTGGTCAATTCAGTTA+AGG | 0.403397 | 1.1:-58781672 | MS.gene74850:CDS |
| AAGCATGGGCCTGCTATTGC+TGG | 0.435283 | 1.1:-58785731 | MS.gene74850:CDS |
| GACTTTGTGCGCAATGGACC+TGG | 0.437274 | 1.1:-58787765 | MS.gene74850:CDS |
| GAGGTACTCCAGCAGGCCTC+TGG | 0.441982 | 1.1:-58786399 | MS.gene74850:intron |
| TGCTTTGGAGGTACTCCAGC+AGG | 0.452022 | 1.1:-58786406 | MS.gene74850:CDS |
| CAGTGGATTCAGATGATCTA+TGG | 0.452319 | 1.1:-58787646 | MS.gene74850:CDS |
| TTGCCTTTCGTAGGATGTCT+GGG | 0.452424 | 1.1:-58786436 | MS.gene74850:intron |
| GTGGTATTGCTGCAATTCTT+CGG | 0.459436 | 1.1:-58781329 | MS.gene74850:CDS |
| CGAAAAGACTTTGTGCGCAA+TGG | 0.462477 | 1.1:-58787771 | MS.gene74850:CDS |
| TTTACCAATAAGGAGGATAT+GGG | 0.463278 | 1.1:+58786036 | None:intergenic |
| GAACATGATAAAAGATACTA+AGG | 0.469528 | 1.1:-58783824 | MS.gene74850:CDS |
| ATAACATACCAGAGGCCTGC+TGG | 0.479042 | 1.1:+58786391 | None:intergenic |
| CTGTAGGCATAGTTTAAAAG+AGG | 0.487997 | 1.1:-58783869 | MS.gene74850:intron |
| AACTGCCCCTCAGGACTCCT+AGG | 0.489903 | 1.1:-58781185 | MS.gene74850:intron |
| ATTACCTTCCTGAGCACAAA+AGG | 0.492792 | 1.1:+58786642 | None:intergenic |
| CTTCCCGCGAACACGCAAAA+CGG | 0.498620 | 1.1:+58786808 | None:intergenic |
| TTGCACAGCTAGGCGTTCAT+GGG | 0.499013 | 1.1:+58782321 | None:intergenic |
| GCTAGATCAGCACTTGCAGC+AGG | 0.502678 | 1.1:+58786085 | None:intergenic |
| GCTTCAATTCCTCGTAAGCA+TGG | 0.505004 | 1.1:-58785746 | MS.gene74850:CDS |
| GTATATAATTTGATAGCTCC+TGG | 0.505012 | 1.1:-58787620 | MS.gene74850:CDS |
| GTTCTTAGAGAAGCAGCTAA+TGG | 0.507930 | 1.1:-58787566 | MS.gene74850:CDS |
| CTCTCGTAACCAGCAATAGC+AGG | 0.515882 | 1.1:+58785722 | None:intergenic |
| ATTCTCACTCACACGACCTC+AGG | 0.527617 | 1.1:-58784362 | MS.gene74850:CDS |
| ACTCAGCATCCCACTTGCCT+AGG | 0.534636 | 1.1:+58781168 | None:intergenic |
| TCTTCAGGTTCGAATTCTGA+AGG | 0.535228 | 1.1:-58783698 | MS.gene74850:intron |
| TCTCCATGTCTTCGAGATCA+GGG | 0.535323 | 1.1:+58781298 | None:intergenic |
| TGTGCTGTAATTGCAAGTCC+TGG | 0.539910 | 1.1:-58784658 | MS.gene74850:CDS |
| AGCTCCTGGTGATTCAATCA+TGG | 0.552812 | 1.1:-58787606 | MS.gene74850:CDS |
| GTATGACTGTTACTCGTTCG+CGG | 0.553104 | 1.1:-58785775 | MS.gene74850:CDS |
| AGATACTAAGGCAGCACAAG+AGG | 0.557963 | 1.1:-58783812 | MS.gene74850:intron |
| CATGCATGGATGAAAATACA+TGG | 0.575010 | 1.1:+58781633 | None:intergenic |
| CTCAGGACTCCTAGGCAAGT+GGG | 0.583005 | 1.1:-58781177 | MS.gene74850:intron |
| CTTCAATTCCTCGTAAGCAT+GGG | 0.583987 | 1.1:-58785745 | MS.gene74850:CDS |
| TCTCCCTGATCTCGAAGACA+TGG | 0.585381 | 1.1:-58781301 | MS.gene74850:CDS |
| CATAGATCATCTGAATCCAC+TGG | 0.593444 | 1.1:+58787647 | None:intergenic |
| TTATGGAACAAAACATGTTG+AGG | 0.595098 | 1.1:-58782347 | MS.gene74850:CDS |
| CCTCAGGACTCCTAGGCAAG+TGG | 0.595778 | 1.1:-58781178 | MS.gene74850:intron |
| TGAGAAGCGTTTGCACAGCT+AGG | 0.600897 | 1.1:+58782311 | None:intergenic |
| AAGAACTTTCCTGATAGTAA+CGG | 0.602859 | 1.1:+58787583 | None:intergenic |
| CTTAGAGAAGCAGCTAATGG+TGG | 0.604388 | 1.1:-58787563 | MS.gene74850:CDS |
| TGATACACAGATGGTGCCAG+TGG | 0.604532 | 1.1:-58787663 | MS.gene74850:intron |
| ACAGAACTAGAGCAGATAAG+TGG | 0.610661 | 1.1:-58781348 | MS.gene74850:intron |
| TCTAGAGAATGAACATGTCA+AGG | 0.615714 | 1.1:-58786780 | MS.gene74850:intron |
| AATTCAGTTAAGGATTCTGG+AGG | 0.623215 | 1.1:-58781662 | MS.gene74850:CDS |
| TGCAAGTGCTGATCTAGCTG+TGG | 0.630199 | 1.1:-58786080 | MS.gene74850:CDS |
| CTTTAGAACTTGAGTTACAG+CGG | 0.635772 | 1.1:-58786665 | MS.gene74850:CDS |
| ATGTCTTCGAGATCAGGGAG+AGG | 0.648204 | 1.1:+58781303 | None:intergenic |
| GCTGTGGTGTTGATGCAAGA+AGG | 0.651031 | 1.1:-58786064 | MS.gene74850:CDS |
| ACGGCCATGATTGAATCACC+AGG | 0.658374 | 1.1:+58787602 | None:intergenic |
| ATAGCAGGCCCATGCTTACG+AGG | 0.660640 | 1.1:+58785737 | None:intergenic |
| GGTGTGGATTATGACAAAGA+AGG | 0.663103 | 1.1:-58786833 | MS.gene74850:CDS |
| GGAATGCAGATATACCTACC+AGG | 0.665150 | 1.1:-58781706 | MS.gene74850:CDS |
| ACGTTCTCCAGAGACATGCA+TGG | 0.686516 | 1.1:+58781619 | None:intergenic |
| GCAACCGTACTTGTATCCTG+AGG | 0.720068 | 1.1:+58784346 | None:intergenic |
| GAGACATATAGATTTCAACG+TGG | 0.809146 | 1.1:-58784686 | MS.gene74850:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 58781170 | 58787805 | 58781170 | ID=MS.gene74850 |
| chr1.1 | mRNA | 58781170 | 58787805 | 58781170 | ID=MS.gene74850.t1;Parent=MS.gene74850 |
| chr1.1 | exon | 58787761 | 58787805 | 58787761 | ID=MS.gene74850.t1.exon1;Parent=MS.gene74850.t1 |
| chr1.1 | CDS | 58787761 | 58787805 | 58787761 | ID=cds.MS.gene74850.t1;Parent=MS.gene74850.t1 |
| chr1.1 | exon | 58787514 | 58787675 | 58787514 | ID=MS.gene74850.t1.exon2;Parent=MS.gene74850.t1 |
| chr1.1 | CDS | 58787514 | 58787675 | 58787514 | ID=cds.MS.gene74850.t1;Parent=MS.gene74850.t1 |
| chr1.1 | exon | 58786781 | 58786855 | 58786781 | ID=MS.gene74850.t1.exon3;Parent=MS.gene74850.t1 |
| chr1.1 | CDS | 58786781 | 58786855 | 58786781 | ID=cds.MS.gene74850.t1;Parent=MS.gene74850.t1 |
| chr1.1 | exon | 58786647 | 58786703 | 58786647 | ID=MS.gene74850.t1.exon4;Parent=MS.gene74850.t1 |
| chr1.1 | CDS | 58786647 | 58786703 | 58786647 | ID=cds.MS.gene74850.t1;Parent=MS.gene74850.t1 |
| chr1.1 | exon | 58786400 | 58786445 | 58786400 | ID=MS.gene74850.t1.exon5;Parent=MS.gene74850.t1 |
| chr1.1 | CDS | 58786400 | 58786445 | 58786400 | ID=cds.MS.gene74850.t1;Parent=MS.gene74850.t1 |
| chr1.1 | exon | 58786034 | 58786109 | 58786034 | ID=MS.gene74850.t1.exon6;Parent=MS.gene74850.t1 |
| chr1.1 | CDS | 58786034 | 58786109 | 58786034 | ID=cds.MS.gene74850.t1;Parent=MS.gene74850.t1 |
| chr1.1 | exon | 58785721 | 58785796 | 58785721 | ID=MS.gene74850.t1.exon7;Parent=MS.gene74850.t1 |
| chr1.1 | CDS | 58785721 | 58785796 | 58785721 | ID=cds.MS.gene74850.t1;Parent=MS.gene74850.t1 |
| chr1.1 | exon | 58785585 | 58785617 | 58785585 | ID=MS.gene74850.t1.exon8;Parent=MS.gene74850.t1 |
| chr1.1 | CDS | 58785585 | 58785617 | 58785585 | ID=cds.MS.gene74850.t1;Parent=MS.gene74850.t1 |
| chr1.1 | exon | 58784648 | 58784716 | 58784648 | ID=MS.gene74850.t1.exon9;Parent=MS.gene74850.t1 |
| chr1.1 | CDS | 58784648 | 58784716 | 58784648 | ID=cds.MS.gene74850.t1;Parent=MS.gene74850.t1 |
| chr1.1 | exon | 58784356 | 58784462 | 58784356 | ID=MS.gene74850.t1.exon10;Parent=MS.gene74850.t1 |
| chr1.1 | CDS | 58784356 | 58784462 | 58784356 | ID=cds.MS.gene74850.t1;Parent=MS.gene74850.t1 |
| chr1.1 | exon | 58783813 | 58783885 | 58783813 | ID=MS.gene74850.t1.exon11;Parent=MS.gene74850.t1 |
| chr1.1 | CDS | 58783813 | 58783885 | 58783813 | ID=cds.MS.gene74850.t1;Parent=MS.gene74850.t1 |
| chr1.1 | exon | 58783675 | 58783713 | 58783675 | ID=MS.gene74850.t1.exon12;Parent=MS.gene74850.t1 |
| chr1.1 | CDS | 58783675 | 58783713 | 58783675 | ID=cds.MS.gene74850.t1;Parent=MS.gene74850.t1 |
| chr1.1 | exon | 58782292 | 58782386 | 58782292 | ID=MS.gene74850.t1.exon13;Parent=MS.gene74850.t1 |
| chr1.1 | CDS | 58782292 | 58782386 | 58782292 | ID=cds.MS.gene74850.t1;Parent=MS.gene74850.t1 |
| chr1.1 | exon | 58781621 | 58781727 | 58781621 | ID=MS.gene74850.t1.exon14;Parent=MS.gene74850.t1 |
| chr1.1 | CDS | 58781621 | 58781727 | 58781621 | ID=cds.MS.gene74850.t1;Parent=MS.gene74850.t1 |
| chr1.1 | exon | 58781297 | 58781366 | 58781297 | ID=MS.gene74850.t1.exon15;Parent=MS.gene74850.t1 |
| chr1.1 | CDS | 58781297 | 58781366 | 58781297 | ID=cds.MS.gene74850.t1;Parent=MS.gene74850.t1 |
| chr1.1 | exon | 58781170 | 58781194 | 58781170 | ID=MS.gene74850.t1.exon16;Parent=MS.gene74850.t1 |
| chr1.1 | CDS | 58781170 | 58781194 | 58781170 | ID=cds.MS.gene74850.t1;Parent=MS.gene74850.t1 |
| Gene Sequence |
| Protein sequence |
