Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07548.t1 | XP_013468922.1 | 98.4 | 379 | 4 | 1 | 1 | 377 | 1 | 379 | 9.20E-208 | 732.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07548.t1 | Q9ZT87 | 78.4 | 379 | 79 | 2 | 1 | 377 | 1 | 378 | 5.9e-171 | 601.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07548.t1 | A0A072VLU0 | 98.4 | 379 | 4 | 1 | 1 | 377 | 1 | 379 | 6.6e-208 | 732.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049230 | MS.gene07548 | 0.819413 | 1.18E-52 | -1.69E-46 |
| MS.gene050642 | MS.gene07548 | 0.849477 | 3.15E-60 | -1.69E-46 |
| MS.gene050987 | MS.gene07548 | 0.823565 | 1.29E-53 | -1.69E-46 |
| MS.gene051373 | MS.gene07548 | 0.801099 | 1.06E-48 | -1.69E-46 |
| MS.gene051983 | MS.gene07548 | 0.848828 | 4.78E-60 | -1.69E-46 |
| MS.gene052077 | MS.gene07548 | 0.809748 | 1.62E-50 | -1.69E-46 |
| MS.gene052176 | MS.gene07548 | 0.832195 | 1.09E-55 | -1.69E-46 |
| MS.gene054349 | MS.gene07548 | 0.801428 | 9.05E-49 | -1.69E-46 |
| MS.gene054930 | MS.gene07548 | 0.834339 | 3.17E-56 | -1.69E-46 |
| MS.gene056659 | MS.gene07548 | 0.815379 | 9.53E-52 | -1.69E-46 |
| MS.gene056979 | MS.gene07548 | 0.838643 | 2.55E-57 | -1.69E-46 |
| MS.gene058142 | MS.gene07548 | 0.845303 | 4.42E-59 | -1.69E-46 |
| MS.gene058435 | MS.gene07548 | 0.801281 | 9.70E-49 | -1.69E-46 |
| MS.gene059285 | MS.gene07548 | 0.81769 | 2.89E-52 | -1.69E-46 |
| MS.gene059710 | MS.gene07548 | 0.80532 | 1.41E-49 | -1.69E-46 |
| MS.gene059899 | MS.gene07548 | 0.821772 | 3.38E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene07548 | MS.gene005004 | PPI |
| MS.gene07548 | MS.gene74850 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07548.t1 | MTR_1g078400 | 98.417 | 379 | 4 | 1 | 1 | 377 | 1 | 379 | 0.0 | 764 |
| MS.gene07548.t1 | MTR_1g078400 | 98.125 | 320 | 4 | 1 | 1 | 318 | 1 | 320 | 0.0 | 645 |
| MS.gene07548.t1 | MTR_7g102760 | 43.622 | 392 | 202 | 6 | 1 | 376 | 1 | 389 | 7.10e-100 | 302 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07548.t1 | AT4G27650 | 78.364 | 379 | 79 | 2 | 1 | 377 | 1 | 378 | 0.0 | 624 |
| MS.gene07548.t1 | AT4G27650 | 78.364 | 379 | 79 | 2 | 1 | 377 | 1 | 378 | 0.0 | 624 |
| MS.gene07548.t1 | AT4G27650 | 78.364 | 379 | 79 | 2 | 1 | 377 | 1 | 378 | 0.0 | 624 |
| MS.gene07548.t1 | AT3G58390 | 70.000 | 400 | 92 | 4 | 1 | 377 | 1 | 395 | 0.0 | 568 |
Find 70 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCAATTCAGTTAAGGATTC+TGG | 0.172762 | 1.2:+62293267 | MS.gene07548:CDS |
| GCATAGTTTAAAAGAGGTTT+TGG | 0.278574 | 1.2:+62291068 | MS.gene07548:CDS |
| TTAGCCCATATCCACCTTAT+TGG | 0.300006 | 1.2:+62288892 | MS.gene07548:CDS |
| ATATCCACCTTATTGGTAAA+AGG | 0.301257 | 1.2:+62288899 | MS.gene07548:CDS |
| TTTATCATGTTCATGACATT+TGG | 0.301926 | 1.2:-62291096 | None:intergenic |
| GTAACTCAAGTTCTAAAGTA+TGG | 0.306803 | 1.2:-62288262 | None:intergenic |
| TTTGCCTTTCGTAGGATGTC+TGG | 0.312960 | 1.2:+62288495 | MS.gene07548:intron |
| AATTGAACCTTAACGCTTCC+AGG | 0.331018 | 1.2:-62287185 | None:intergenic |
| TGAACGTGTTAAACTCAAAT+TGG | 0.335052 | 1.2:+62287401 | MS.gene07548:CDS |
| TGTCTGGGATTCTTATGCTT+TGG | 0.353497 | 1.2:+62288511 | MS.gene07548:CDS |
| GATTCATCACGTGCTTGTTA+TGG | 0.355531 | 1.2:+62292568 | MS.gene07548:CDS |
| TTTGCACAGCTAGGCGTTCA+TGG | 0.362499 | 1.2:-62292612 | None:intergenic |
| CAATCATGGCCGTTACTATC+AGG | 0.364180 | 1.2:+62287340 | MS.gene07548:CDS |
| ATCTCCATGTCTTCGAGATC+AGG | 0.375283 | 1.2:-62293635 | None:intergenic |
| CTGGGATTCTTATGCTTTGG+AGG | 0.376577 | 1.2:+62288514 | MS.gene07548:CDS |
| TAACTTGGTCAATTCAGTTA+AGG | 0.403397 | 1.2:+62293260 | MS.gene07548:CDS |
| AAGCATGGGCCTGCTATTGC+TGG | 0.435283 | 1.2:+62289200 | MS.gene07548:CDS |
| GACTTTGTGCGCAATGGACC+TGG | 0.437274 | 1.2:+62287167 | MS.gene07548:CDS |
| GAGGTACTCCAGCAGGCCTC+TGG | 0.441982 | 1.2:+62288533 | MS.gene07548:CDS |
| TTTACCAATAAGGTGGATAT+GGG | 0.443876 | 1.2:-62288896 | None:intergenic |
| TGCTTTGGAGGTACTCCAGC+AGG | 0.452022 | 1.2:+62288526 | MS.gene07548:CDS |
| CAGTGGATTCAGATGATCTA+TGG | 0.452319 | 1.2:+62287286 | MS.gene07548:CDS |
| TTGCCTTTCGTAGGATGTCT+GGG | 0.452424 | 1.2:+62288496 | MS.gene07548:intron |
| GTGGTATTGCTGCAATTCTT+CGG | 0.459436 | 1.2:+62293603 | MS.gene07548:CDS |
| CGAAAAGACTTTGTGCGCAA+TGG | 0.462477 | 1.2:+62287161 | MS.gene07548:CDS |
| GAACATGATAAAAGATACTA+AGG | 0.469528 | 1.2:+62291107 | MS.gene07548:CDS |
| ATAACATACCAGAGGCCTGC+TGG | 0.479042 | 1.2:-62288541 | None:intergenic |
| CTGTAGGCATAGTTTAAAAG+AGG | 0.487997 | 1.2:+62291062 | MS.gene07548:intron |
| AACTGCCCCTCAGGACTCCT+AGG | 0.489903 | 1.2:+62293747 | MS.gene07548:intron |
| ATTACCTTCCTGAGCACAAA+AGG | 0.492792 | 1.2:-62288290 | None:intergenic |
| CTTCCCGCGAACACGCAAAA+CGG | 0.498620 | 1.2:-62288124 | None:intergenic |
| TTGCACAGCTAGGCGTTCAT+GGG | 0.499013 | 1.2:-62292611 | None:intergenic |
| GCTAGATCAGCACTTGCAGC+AGG | 0.502678 | 1.2:-62288847 | None:intergenic |
| GCTTCAATTCCTCGTAAGCA+TGG | 0.505004 | 1.2:+62289185 | MS.gene07548:CDS |
| GTATATAATTTGATAGCTCC+TGG | 0.505012 | 1.2:+62287312 | MS.gene07548:CDS |
| GTTCTTAGAGAAGCAGCTAA+TGG | 0.507930 | 1.2:+62287366 | MS.gene07548:CDS |
| CTCTCGTAACCAGCAATAGC+AGG | 0.515882 | 1.2:-62289209 | None:intergenic |
| ATTCTCACTCACACGACCTC+AGG | 0.527617 | 1.2:+62290569 | MS.gene07548:CDS |
| ACTCAGCATCCCACTTGCCT+AGG | 0.534636 | 1.2:-62293764 | None:intergenic |
| TCTTCAGGTTCGAATTCTGA+AGG | 0.535228 | 1.2:+62291233 | MS.gene07548:intron |
| TCTCCATGTCTTCGAGATCA+GGG | 0.535323 | 1.2:-62293634 | None:intergenic |
| TGTGCTGTAATTGCAAGTCC+TGG | 0.539910 | 1.2:+62290273 | MS.gene07548:CDS |
| AGCTCCTGGTGATTCAATCA+TGG | 0.552812 | 1.2:+62287326 | MS.gene07548:CDS |
| GTATGACTGTTACTCGTTCG+CGG | 0.553104 | 1.2:+62289156 | MS.gene07548:intron |
| AGATACTAAGGCAGCACAAG+AGG | 0.557963 | 1.2:+62291119 | MS.gene07548:CDS |
| CATGCATGGATGAAAATACA+TGG | 0.575010 | 1.2:-62293299 | None:intergenic |
| CTCAGGACTCCTAGGCAAGT+GGG | 0.583005 | 1.2:+62293755 | MS.gene07548:intron |
| CTTCAATTCCTCGTAAGCAT+GGG | 0.583987 | 1.2:+62289186 | MS.gene07548:CDS |
| TCTCCCTGATCTCGAAGACA+TGG | 0.585381 | 1.2:+62293631 | MS.gene07548:CDS |
| CATAGATCATCTGAATCCAC+TGG | 0.593444 | 1.2:-62287285 | None:intergenic |
| TTATGGAACAAAACATGTTG+AGG | 0.595098 | 1.2:+62292585 | MS.gene07548:CDS |
| CCTCAGGACTCCTAGGCAAG+TGG | 0.595778 | 1.2:+62293754 | MS.gene07548:intron |
| TGAGAAGCGTTTGCACAGCT+AGG | 0.600897 | 1.2:-62292621 | None:intergenic |
| AAGAACTTTCCTGATAGTAA+CGG | 0.602859 | 1.2:-62287349 | None:intergenic |
| CTTAGAGAAGCAGCTAATGG+TGG | 0.604388 | 1.2:+62287369 | MS.gene07548:CDS |
| TGATACACAGATGGTGCCAG+TGG | 0.604532 | 1.2:+62287269 | MS.gene07548:intron |
| ACAGAACTAGAGCAGATAAG+TGG | 0.610661 | 1.2:+62293584 | MS.gene07548:intron |
| TCTAGAGAATGAACATGTCA+AGG | 0.615714 | 1.2:+62288152 | MS.gene07548:CDS |
| AATTCAGTTAAGGATTCTGG+AGG | 0.623215 | 1.2:+62293270 | MS.gene07548:CDS |
| TGCAAGTGCTGATCTAGCTG+TGG | 0.630199 | 1.2:+62288852 | MS.gene07548:CDS |
| CTTTAGAACTTGAGTTACAG+CGG | 0.635772 | 1.2:+62288267 | MS.gene07548:CDS |
| ATGTCTTCGAGATCAGGGAG+AGG | 0.648204 | 1.2:-62293629 | None:intergenic |
| GCTGTGGTGTTGATGCAAGA+AGG | 0.651031 | 1.2:+62288868 | MS.gene07548:CDS |
| ACGGCCATGATTGAATCACC+AGG | 0.658374 | 1.2:-62287330 | None:intergenic |
| ATAGCAGGCCCATGCTTACG+AGG | 0.660640 | 1.2:-62289194 | None:intergenic |
| GGTGTGGATTATGACAAAGA+AGG | 0.663103 | 1.2:+62288099 | MS.gene07548:CDS |
| GGAATGCAGATATACCTACC+AGG | 0.665150 | 1.2:+62293226 | MS.gene07548:intron |
| ACGTTCTCCAGAGACATGCA+TGG | 0.686516 | 1.2:-62293313 | None:intergenic |
| GCAACCGTACTTGTATCCTG+AGG | 0.720068 | 1.2:-62290585 | None:intergenic |
| GAGACATATAGATTTCAACG+TGG | 0.809146 | 1.2:+62290245 | MS.gene07548:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 62287149 | 62293784 | 62287149 | ID=MS.gene07548 |
| chr1.2 | mRNA | 62287149 | 62293784 | 62287149 | ID=MS.gene07548.t1;Parent=MS.gene07548 |
| chr1.2 | exon | 62287149 | 62287193 | 62287149 | ID=MS.gene07548.t1.exon1;Parent=MS.gene07548.t1 |
| chr1.2 | CDS | 62287149 | 62287193 | 62287149 | ID=cds.MS.gene07548.t1;Parent=MS.gene07548.t1 |
| chr1.2 | exon | 62287279 | 62287440 | 62287279 | ID=MS.gene07548.t1.exon2;Parent=MS.gene07548.t1 |
| chr1.2 | CDS | 62287279 | 62287440 | 62287279 | ID=cds.MS.gene07548.t1;Parent=MS.gene07548.t1 |
| chr1.2 | exon | 62288099 | 62288173 | 62288099 | ID=MS.gene07548.t1.exon3;Parent=MS.gene07548.t1 |
| chr1.2 | CDS | 62288099 | 62288173 | 62288099 | ID=cds.MS.gene07548.t1;Parent=MS.gene07548.t1 |
| chr1.2 | exon | 62288251 | 62288307 | 62288251 | ID=MS.gene07548.t1.exon4;Parent=MS.gene07548.t1 |
| chr1.2 | CDS | 62288251 | 62288307 | 62288251 | ID=cds.MS.gene07548.t1;Parent=MS.gene07548.t1 |
| chr1.2 | exon | 62288509 | 62288554 | 62288509 | ID=MS.gene07548.t1.exon5;Parent=MS.gene07548.t1 |
| chr1.2 | CDS | 62288509 | 62288554 | 62288509 | ID=cds.MS.gene07548.t1;Parent=MS.gene07548.t1 |
| chr1.2 | exon | 62288845 | 62288920 | 62288845 | ID=MS.gene07548.t1.exon6;Parent=MS.gene07548.t1 |
| chr1.2 | CDS | 62288845 | 62288920 | 62288845 | ID=cds.MS.gene07548.t1;Parent=MS.gene07548.t1 |
| chr1.2 | exon | 62289157 | 62289232 | 62289157 | ID=MS.gene07548.t1.exon7;Parent=MS.gene07548.t1 |
| chr1.2 | CDS | 62289157 | 62289232 | 62289157 | ID=cds.MS.gene07548.t1;Parent=MS.gene07548.t1 |
| chr1.2 | exon | 62289336 | 62289368 | 62289336 | ID=MS.gene07548.t1.exon8;Parent=MS.gene07548.t1 |
| chr1.2 | CDS | 62289336 | 62289368 | 62289336 | ID=cds.MS.gene07548.t1;Parent=MS.gene07548.t1 |
| chr1.2 | exon | 62290237 | 62290305 | 62290237 | ID=MS.gene07548.t1.exon9;Parent=MS.gene07548.t1 |
| chr1.2 | CDS | 62290237 | 62290305 | 62290237 | ID=cds.MS.gene07548.t1;Parent=MS.gene07548.t1 |
| chr1.2 | exon | 62290491 | 62290597 | 62290491 | ID=MS.gene07548.t1.exon10;Parent=MS.gene07548.t1 |
| chr1.2 | CDS | 62290491 | 62290597 | 62290491 | ID=cds.MS.gene07548.t1;Parent=MS.gene07548.t1 |
| chr1.2 | exon | 62291068 | 62291140 | 62291068 | ID=MS.gene07548.t1.exon11;Parent=MS.gene07548.t1 |
| chr1.2 | CDS | 62291068 | 62291140 | 62291068 | ID=cds.MS.gene07548.t1;Parent=MS.gene07548.t1 |
| chr1.2 | exon | 62291240 | 62291278 | 62291240 | ID=MS.gene07548.t1.exon12;Parent=MS.gene07548.t1 |
| chr1.2 | CDS | 62291240 | 62291278 | 62291240 | ID=cds.MS.gene07548.t1;Parent=MS.gene07548.t1 |
| chr1.2 | exon | 62292568 | 62292662 | 62292568 | ID=MS.gene07548.t1.exon13;Parent=MS.gene07548.t1 |
| chr1.2 | CDS | 62292568 | 62292662 | 62292568 | ID=cds.MS.gene07548.t1;Parent=MS.gene07548.t1 |
| chr1.2 | exon | 62293227 | 62293333 | 62293227 | ID=MS.gene07548.t1.exon14;Parent=MS.gene07548.t1 |
| chr1.2 | CDS | 62293227 | 62293333 | 62293227 | ID=cds.MS.gene07548.t1;Parent=MS.gene07548.t1 |
| chr1.2 | exon | 62293588 | 62293657 | 62293588 | ID=MS.gene07548.t1.exon15;Parent=MS.gene07548.t1 |
| chr1.2 | CDS | 62293588 | 62293657 | 62293588 | ID=cds.MS.gene07548.t1;Parent=MS.gene07548.t1 |
| chr1.2 | exon | 62293760 | 62293784 | 62293760 | ID=MS.gene07548.t1.exon16;Parent=MS.gene07548.t1 |
| chr1.2 | CDS | 62293760 | 62293784 | 62293760 | ID=cds.MS.gene07548.t1;Parent=MS.gene07548.t1 |
| Gene Sequence |
| Protein sequence |
