AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene74933


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74933.t1 MTR_1g112700 96.154 182 7 0 1 182 1 182 3.14e-131 365
MS.gene74933.t1 MTR_3g099580 39.216 102 60 2 29 130 22 121 3.10e-22 89.0
MS.gene74933.t1 MTR_3g099570 34.459 148 81 4 18 161 7 142 1.03e-21 87.4
MS.gene74933.t1 MTR_8g007020 35.772 123 73 4 9 129 2 120 7.75e-20 82.4
MS.gene74933.t1 MTR_8g007035 37.398 123 71 4 9 129 2 120 2.52e-19 81.6
MS.gene74933.t1 MTR_8g089110 37.607 117 67 4 15 129 8 120 1.02e-18 77.8
MS.gene74933.t1 MTR_6g013200 36.667 120 72 3 14 130 14 132 1.13e-18 79.3
MS.gene74933.t1 MTR_3g099680 35.294 119 71 3 18 131 7 124 1.29e-18 80.1
MS.gene74933.t1 MTR_1g104800 32.520 123 76 3 13 133 9 126 3.94e-18 78.2
MS.gene74933.t1 MTR_3g099980 34.454 119 72 3 18 131 7 124 1.53e-17 77.4
MS.gene74933.t1 MTR_5g006040 35.294 102 63 2 30 129 23 123 2.09e-17 76.3
MS.gene74933.t1 MTR_7g086280 30.894 123 80 2 14 134 7 126 2.25e-17 75.9
MS.gene74933.t1 MTR_1g090190 33.058 121 71 3 30 150 20 130 3.73e-17 75.5
MS.gene74933.t1 MTR_4g114870 30.719 153 89 4 7 155 5 144 5.23e-17 77.8
MS.gene74933.t1 MTR_4g114870 36.634 101 59 2 33 129 191 290 2.48e-16 75.9
MS.gene74933.t1 MTR_7g086230 29.268 123 82 2 14 134 7 126 1.77e-16 73.6
MS.gene74933.t1 MTR_6g080660 31.092 119 80 2 17 135 12 128 2.78e-16 73.6
MS.gene74933.t1 MTR_1g105130 35.484 124 77 3 11 132 2 124 4.76e-16 72.0
MS.gene74933.t1 MTR_1g105120 35.484 124 77 3 11 132 2 124 4.76e-16 72.0
MS.gene74933.t1 MTR_7g086160 34.821 112 70 2 8 119 4 112 2.49e-15 70.1
MS.gene74933.t1 MTR_7g086100 33.333 123 75 3 14 132 7 126 3.66e-15 71.2
MS.gene74933.t1 MTR_7g086220 32.479 117 77 2 14 129 6 121 5.37e-15 69.3
MS.gene74933.t1 MTR_8g088830 37.255 102 61 2 28 129 23 121 6.59e-15 68.2
MS.gene74933.t1 MTR_7g086140 33.929 112 70 2 8 119 4 111 6.62e-15 68.9
MS.gene74933.t1 MTR_7g086190 33.929 112 70 2 8 119 4 111 6.62e-15 68.9
MS.gene74933.t1 MTR_6g013420 33.607 122 77 3 11 130 16 135 7.06e-15 69.3
MS.gene74933.t1 MTR_3g089035 37.755 98 57 3 30 127 30 123 2.18e-14 66.6
MS.gene74933.t1 MTR_4g007250 33.654 104 67 2 28 131 24 125 3.36e-14 67.4
MS.gene74933.t1 MTR_8g463180 29.167 120 82 2 12 129 3 121 5.59e-14 68.9
MS.gene74933.t1 MTR_2g088990 31.897 116 73 4 17 128 6 119 1.10e-13 67.0
MS.gene74933.t1 MTR_7g086090 32.231 121 77 2 13 131 6 123 1.10e-13 67.4
MS.gene74933.t1 MTR_6g023760 33.010 103 66 3 28 129 21 121 5.23e-13 64.3
MS.gene74933.t1 MTR_3g089005 33.333 117 72 4 9 125 11 121 5.30e-13 63.2
MS.gene74933.t1 MTR_4g067200 28.571 126 84 3 13 133 9 133 7.84e-13 63.5
MS.gene74933.t1 MTR_4g066110 28.571 126 84 3 13 133 9 133 7.84e-13 63.5
MS.gene74933.t1 MTR_6g013170 27.778 144 88 3 12 153 8 137 1.82e-12 62.8
MS.gene74933.t1 MTR_4g130780 28.369 141 88 4 13 148 9 141 3.09e-12 62.4
MS.gene74933.t1 MTR_2g043730 31.933 119 77 3 11 129 10 124 8.18e-12 60.1
MS.gene74933.t1 MTR_2g090575 30.000 120 79 3 12 129 6 122 3.58e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74933.t1 AT2G31050 38.462 143 75 4 11 152 8 138 2.69e-23 92.0
MS.gene74933.t1 AT3G17675 35.294 119 72 2 15 129 10 127 1.74e-20 82.8
MS.gene74933.t1 AT5G26330 35.294 102 63 2 30 129 21 121 1.21e-18 79.3
MS.gene74933.t1 AT1G72230 30.769 169 104 6 8 171 3 163 7.39e-18 77.4
MS.gene74933.t1 AT2G26720 36.220 127 78 3 8 133 5 129 2.27e-17 76.6
MS.gene74933.t1 AT2G32300 30.769 117 80 1 13 129 6 121 8.69e-17 75.9
MS.gene74933.t1 AT5G07475 28.082 146 101 3 12 155 9 152 1.03e-16 74.7
MS.gene74933.t1 AT2G32300 27.044 159 109 2 13 171 36 187 1.58e-16 75.9
MS.gene74933.t1 AT2G02850 34.211 114 67 4 14 127 21 126 4.71e-16 71.2
MS.gene74933.t1 AT2G25060 28.667 150 97 4 16 156 14 162 3.36e-15 70.5
MS.gene74933.t1 AT5G15350 30.400 125 81 3 12 131 4 127 3.79e-15 70.1
MS.gene74933.t1 AT5G20230 30.645 124 82 2 33 153 25 147 3.21e-14 68.2
MS.gene74933.t1 AT4G31840 27.273 154 97 5 9 151 3 152 3.23e-14 67.4
MS.gene74933.t1 AT1G22480 32.773 119 78 2 18 136 4 120 3.80e-14 67.4
MS.gene74933.t1 AT2G27035 29.310 116 80 2 36 150 32 146 4.52e-14 67.0
MS.gene74933.t1 AT1G45063 33.600 125 74 4 13 132 9 129 5.04e-14 68.6
MS.gene74933.t1 AT1G45063 33.600 125 74 4 13 132 9 129 1.31e-13 68.2
MS.gene74933.t1 AT4G12880 32.479 117 75 3 16 130 12 126 2.33e-13 64.3
MS.gene74933.t1 AT3G60270 33.010 103 67 2 27 129 20 120 2.61e-13 65.5
MS.gene74933.t1 AT4G32490 26.667 120 85 2 12 129 10 128 4.84e-13 65.1
MS.gene74933.t1 AT1G17800 30.833 120 78 2 13 128 17 135 5.74e-13 63.5
MS.gene74933.t1 AT3G27200 29.661 118 80 3 13 129 6 121 2.01e-12 62.8
MS.gene74933.t1 AT5G53870 30.000 160 97 6 13 159 1 158 2.39e-12 64.7
MS.gene74933.t1 AT5G53870 30.000 160 97 6 13 159 1 158 3.11e-12 64.3
MS.gene74933.t1 AT4G28365 31.313 99 65 2 33 129 29 126 3.26e-12 62.8
MS.gene74933.t1 AT3G60280 32.039 103 68 2 27 129 18 118 5.56e-12 62.4
MS.gene74933.t1 AT3G20570 29.545 132 82 3 18 139 8 138 1.33e-11 61.2
MS.gene74933.t1 AT2G44790 33.333 96 60 3 40 133 35 128 4.77e-11 59.7
MS.gene74933.t1 AT3G18590 29.839 124 76 5 13 129 7 126 7.69e-11 58.5

Find 34 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCCTTGGAGGAATGAGGTT+TGG 0.332332 1.3:-76461779 MS.gene74933:CDS
CCCATGTAGCATAATTTGTT+TGG 0.336122 1.3:+76462253 None:intergenic
GCCTTGGAGGAATGAGGTTT+GGG 0.340464 1.3:-76461778 MS.gene74933:CDS
AAGTTAGTTCCTTTCGGAAC+TGG 0.360694 1.3:-76461635 MS.gene74933:CDS
GATGGAGTTGGTTGAATTTG+TGG 0.383777 1.3:+76461698 None:intergenic
TCTCAAAGATGGAGAGTGAT+TGG 0.389696 1.3:-76461659 MS.gene74933:CDS
TTTAGCCGTGGAGATGTTCT+TGG 0.401037 1.3:-76462216 MS.gene74933:intron
ATTGGGAAGTTAGTTCCTTT+CGG 0.402044 1.3:-76461641 MS.gene74933:CDS
TAAATGAGGTGAAGAGATAT+TGG 0.410957 1.3:-76461826 MS.gene74933:CDS
TGGTTCATTTGCAATATAGC+TGG 0.431102 1.3:-76461806 MS.gene74933:CDS
TGGATTTAATGCACCGTCCA+TGG 0.449414 1.3:+76461718 None:intergenic
ATAGCTGGACATTGCCTTGG+AGG 0.459542 1.3:-76461791 MS.gene74933:CDS
ATGGCTAGTGTTTATGCTGT+TGG 0.480177 1.3:-76462297 MS.gene74933:CDS
CTCAAAGATGGAGAGTGATT+GGG 0.483596 1.3:-76461658 MS.gene74933:CDS
ATTCTTGTATCTCTCAAAGA+TGG 0.484655 1.3:-76461670 MS.gene74933:CDS
GACATTGCCTTGGAGGAATG+AGG 0.494262 1.3:-76461784 MS.gene74933:CDS
GCCAAACAAATTATGCTACA+TGG 0.511548 1.3:-76462254 MS.gene74933:CDS
CAACAATGGTACTAATTCCA+TGG 0.540787 1.3:-76461735 MS.gene74933:CDS
AATATAGCTGGACATTGCCT+TGG 0.550956 1.3:-76461794 MS.gene74933:CDS
AGATCAAGTGGTTCTAAATG+AGG 0.556619 1.3:-76461840 MS.gene74933:CDS
CTGTTGGTGATCAAGATGAA+TGG 0.566698 1.3:-76462281 MS.gene74933:CDS
AATAAGAATTGAGAGTATGA+AGG 0.571197 1.3:+76462350 None:intergenic
GTGTTGGCTAAGTATGAAAG+TGG 0.577082 1.3:-76461866 MS.gene74933:CDS
GTCAAAGAAGATAGCAACAA+TGG 0.584763 1.3:-76461749 MS.gene74933:CDS
TAATGTGTATGAAGTGAGAG+AGG 0.584918 1.3:-76461921 MS.gene74933:CDS
CCAAACAAATTATGCTACAT+GGG 0.597055 1.3:-76462253 MS.gene74933:CDS
AATAATAATCCAGTTCCGAA+AGG 0.605908 1.3:+76461626 None:intergenic
TGAAACAAGTAGTGGAGTGT+TGG 0.609215 1.3:-76461882 MS.gene74933:CDS
AGATCATGTGAAACAAGTAG+TGG 0.611448 1.3:-76461890 MS.gene74933:CDS
AATGGTACTAATTCCATGGA+CGG 0.621827 1.3:-76461731 MS.gene74933:CDS
GAGAGATACAACTTTAGCCG+TGG 0.623416 1.3:-76462228 MS.gene74933:CDS
TCCCAAACCTCATTCCTCCA+AGG 0.624087 1.3:+76461777 None:intergenic
TGAAAGTGGAGAAGATCAAG+TGG 0.654735 1.3:-76461852 MS.gene74933:CDS
TACTACCAAGAACATCTCCA+CGG 0.676544 1.3:+76462211 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAATTAAATTATATTTT+TGG - chr1.3:76462003-76462022 MS.gene74933:intron 0.0%
!!! TTATATTTTTGGTGTTGTAT+TGG - chr1.3:76462014-76462033 MS.gene74933:intron 20.0%
! AAGATAATGTTATGATCACA+TGG + chr1.3:76461886-76461905 None:intergenic 25.0%
!! AATAAGAATTGAGAGTATGA+AGG + chr1.3:76461641-76461660 None:intergenic 25.0%
!! ATCACATGGATATCTTTTAT+TGG + chr1.3:76461872-76461891 None:intergenic 25.0%
!!! ATAGTTTTCAAGTATGTGAA+GGG - chr1.3:76462038-76462057 MS.gene74933:intron 25.0%
!!! TATAGTTTTCAAGTATGTGA+AGG - chr1.3:76462037-76462056 MS.gene74933:intron 25.0%
AATAATAATCCAGTTCCGAA+AGG + chr1.3:76462365-76462384 None:intergenic 30.0%
ATTCTTGTATCTCTCAAAGA+TGG - chr1.3:76462318-76462337 MS.gene74933:CDS 30.0%
CCAAACAAATTATGCTACAT+GGG - chr1.3:76461735-76461754 MS.gene74933:CDS 30.0%
TAAATGAGGTGAAGAGATAT+TGG - chr1.3:76462162-76462181 MS.gene74933:intron 30.0%
! GAGATACAAGAATTTTGTGA+TGG + chr1.3:76462311-76462330 None:intergenic 30.0%
! TTTTCTCTTGAATTGTGTCA+TGG - chr1.3:76461672-76461691 MS.gene74933:CDS 30.0%
!!! TAGTTTTCAAGTATGTGAAG+GGG - chr1.3:76462039-76462058 MS.gene74933:intron 30.0%
AATGGTACTAATTCCATGGA+CGG - chr1.3:76462257-76462276 MS.gene74933:CDS 35.0%
AGATCATGTGAAACAAGTAG+TGG - chr1.3:76462098-76462117 MS.gene74933:intron 35.0%
ATTGGGAAGTTAGTTCCTTT+CGG - chr1.3:76462347-76462366 MS.gene74933:CDS 35.0%
CAACAATGGTACTAATTCCA+TGG - chr1.3:76462253-76462272 MS.gene74933:CDS 35.0%
GCCAAACAAATTATGCTACA+TGG - chr1.3:76461734-76461753 MS.gene74933:CDS 35.0%
GTCAAAGAAGATAGCAACAA+TGG - chr1.3:76462239-76462258 MS.gene74933:CDS 35.0%
TAATGTGTATGAAGTGAGAG+AGG - chr1.3:76462067-76462086 MS.gene74933:intron 35.0%
TGGTTCATTTGCAATATAGC+TGG - chr1.3:76462182-76462201 MS.gene74933:intron 35.0%
!! AGATCAAGTGGTTCTAAATG+AGG - chr1.3:76462148-76462167 MS.gene74933:intron 35.0%
!! CAAGAATTTTGTGATGGAGT+TGG + chr1.3:76462305-76462324 None:intergenic 35.0%
!! CCCATGTAGCATAATTTGTT+TGG + chr1.3:76461738-76461757 None:intergenic 35.0%
AAGTTAGTTCCTTTCGGAAC+TGG - chr1.3:76462353-76462372 MS.gene74933:CDS 40.0%
AATATAGCTGGACATTGCCT+TGG - chr1.3:76462194-76462213 MS.gene74933:intron 40.0%
ATGGCTAGTGTTTATGCTGT+TGG - chr1.3:76461691-76461710 MS.gene74933:CDS 40.0%
CTCAAAGATGGAGAGTGATT+GGG - chr1.3:76462330-76462349 MS.gene74933:CDS 40.0%
TACTACCAAGAACATCTCCA+CGG + chr1.3:76461780-76461799 None:intergenic 40.0%
TCTCAAAGATGGAGAGTGAT+TGG - chr1.3:76462329-76462348 MS.gene74933:CDS 40.0%
TGAAAGTGGAGAAGATCAAG+TGG - chr1.3:76462136-76462155 MS.gene74933:intron 40.0%
! GATGGAGTTGGTTGAATTTG+TGG + chr1.3:76462293-76462312 None:intergenic 40.0%
! TGAAACAAGTAGTGGAGTGT+TGG - chr1.3:76462106-76462125 MS.gene74933:intron 40.0%
!! CTGTTGGTGATCAAGATGAA+TGG - chr1.3:76461707-76461726 MS.gene74933:CDS 40.0%
!! GTGTTGGCTAAGTATGAAAG+TGG - chr1.3:76462122-76462141 MS.gene74933:intron 40.0%
GAGAGATACAACTTTAGCCG+TGG - chr1.3:76461760-76461779 MS.gene74933:CDS 45.0%
TGGATTTAATGCACCGTCCA+TGG + chr1.3:76462273-76462292 None:intergenic 45.0%
TTTAGCCGTGGAGATGTTCT+TGG - chr1.3:76461772-76461791 MS.gene74933:CDS 45.0%
ATAGCTGGACATTGCCTTGG+AGG - chr1.3:76462197-76462216 MS.gene74933:intron 50.0%
GACATTGCCTTGGAGGAATG+AGG - chr1.3:76462204-76462223 MS.gene74933:intron 50.0%
GCCTTGGAGGAATGAGGTTT+GGG - chr1.3:76462210-76462229 MS.gene74933:intron 50.0%
TCCCAAACCTCATTCCTCCA+AGG + chr1.3:76462214-76462233 None:intergenic 50.0%
TGCCTTGGAGGAATGAGGTT+TGG - chr1.3:76462209-76462228 MS.gene74933:intron 50.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 76461607 76462403 76461607 ID=MS.gene74933
chr1.3 mRNA 76461607 76462403 76461607 ID=MS.gene74933.t1;Parent=MS.gene74933
chr1.3 exon 76462217 76462403 76462217 ID=MS.gene74933.t1.exon1;Parent=MS.gene74933.t1
chr1.3 CDS 76462217 76462403 76462217 ID=cds.MS.gene74933.t1;Parent=MS.gene74933.t1
chr1.3 exon 76461607 76461968 76461607 ID=MS.gene74933.t1.exon2;Parent=MS.gene74933.t1
chr1.3 CDS 76461607 76461968 76461607 ID=cds.MS.gene74933.t1;Parent=MS.gene74933.t1
Gene Sequence

>MS.gene74933

ATGGTGATCACAAATTTCTTCAACAACTGTACCTTCATACTCTCAATTCTTATTTTGCTATGTAGTTTTCTCTTGAATTGTGTCATGGCTAGTGTTTATGCTGTTGGTGATCAAGATGAATGGAGTAGCCAAACAAATTATGCTACATGGGCAGAGAGATACAACTTTAGCCGTGGAGATGTTCTTGGTAGTACTTCTCAATTCAATCTTTACTAAATTCTTGTATTTGATAAGTATATATTTTCAAAATATATTCTTTTCACCAATAAAAGATATCCATGTGATCATAACATTATCTTTAAAAAATAAAGTGCTTATTGTGTTTAGTCTATTAAATTTTGAAAGTATAATTAAAATTTAATATGTAGTTTTGAATTTATATTCAAAGTTGATTTATTTTAATTAAATTATATTTTTGGTGTTGTATTGGTATAGTTTTCAAGTATGTGAAGGGGCAACATAATGTGTATGAAGTGAGAGAGGAAACTTTTAGATCATGTGAAACAAGTAGTGGAGTGTTGGCTAAGTATGAAAGTGGAGAAGATCAAGTGGTTCTAAATGAGGTGAAGAGATATTGGTTCATTTGCAATATAGCTGGACATTGCCTTGGAGGAATGAGGTTTGGGATTGAAGTCAAAGAAGATAGCAACAATGGTACTAATTCCATGGACGGTGCATTAAATCCACAAATTCAACCAACTCCATCACAAAATTCTTGTATCTCTCAAAGATGGAGAGTGATTGGGAAGTTAGTTCCTTTCGGAACTGGATTATTATTGCTCAATTTGTATTTTTGA

Protein sequence

>MS.gene74933.t1

MVITNFFNNCTFILSILILLCSFLLNCVMASVYAVGDQDEWSSQTNYATWAERYNFSRGDVLVFKYVKGQHNVYEVREETFRSCETSSGVLAKYESGEDQVVLNEVKRYWFICNIAGHCLGGMRFGIEVKEDSNNGTNSMDGALNPQIQPTPSQNSCISQRWRVIGKLVPFGTGLLLLNLYF