Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75429.t1 | XP_003602037.2 | 88.2 | 212 | 23 | 2 | 1 | 211 | 29 | 239 | 4.90E-62 | 247.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75429.t1 | Q9LT17 | 51.1 | 92 | 43 | 2 | 113 | 203 | 244 | 334 | 1.8e-20 | 100.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75429.t1 | G7J4X5 | 88.2 | 212 | 23 | 2 | 1 | 211 | 1 | 211 | 3.5e-62 | 247.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051247 | MS.gene75429 | 0.804528 | 2.07E-49 | -1.69E-46 |
| MS.gene051744 | MS.gene75429 | 0.812089 | 5.05E-51 | -1.69E-46 |
| MS.gene055818 | MS.gene75429 | 0.80802 | 3.80E-50 | -1.69E-46 |
| MS.gene056050 | MS.gene75429 | 0.803206 | 3.89E-49 | -1.69E-46 |
| MS.gene056174 | MS.gene75429 | 0.800884 | 1.17E-48 | -1.69E-46 |
| MS.gene05847 | MS.gene75429 | 0.803028 | 4.24E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75429.t1 | MTR_3g088100 | 89.623 | 212 | 20 | 2 | 1 | 211 | 1 | 211 | 8.25e-128 | 359 |
| MS.gene75429.t1 | MTR_1g114240 | 39.286 | 196 | 85 | 8 | 11 | 203 | 170 | 334 | 5.99e-29 | 110 |
| MS.gene75429.t1 | MTR_1g114240 | 37.755 | 196 | 82 | 7 | 11 | 203 | 170 | 328 | 3.88e-28 | 108 |
| MS.gene75429.t1 | MTR_4g065670 | 42.991 | 107 | 59 | 2 | 97 | 201 | 134 | 240 | 2.24e-25 | 99.4 |
| MS.gene75429.t1 | MTR_4g065670 | 44.118 | 102 | 55 | 2 | 102 | 201 | 146 | 247 | 8.27e-25 | 98.2 |
| MS.gene75429.t1 | MTR_8g037150 | 39.806 | 103 | 58 | 2 | 102 | 201 | 139 | 240 | 1.22e-24 | 97.4 |
| MS.gene75429.t1 | MTR_8g036640 | 40.000 | 95 | 53 | 2 | 102 | 193 | 137 | 230 | 2.29e-20 | 87.0 |
| MS.gene75429.t1 | MTR_6g082620 | 41.346 | 104 | 58 | 2 | 101 | 201 | 253 | 356 | 1.21e-19 | 85.9 |
| MS.gene75429.t1 | MTR_2g076290 | 38.217 | 157 | 74 | 5 | 67 | 203 | 177 | 330 | 7.25e-19 | 83.6 |
| MS.gene75429.t1 | MTR_2g078440 | 42.857 | 119 | 65 | 2 | 85 | 203 | 184 | 299 | 8.89e-19 | 83.2 |
| MS.gene75429.t1 | MTR_4g085630 | 40.206 | 97 | 56 | 1 | 103 | 197 | 454 | 550 | 2.44e-16 | 77.4 |
| MS.gene75429.t1 | MTR_1g066400 | 36.975 | 119 | 64 | 4 | 81 | 197 | 415 | 524 | 2.21e-15 | 74.3 |
| MS.gene75429.t1 | MTR_3g025940 | 33.600 | 125 | 81 | 1 | 75 | 197 | 316 | 440 | 6.45e-15 | 72.8 |
| MS.gene75429.t1 | MTR_2g078450 | 35.211 | 142 | 74 | 5 | 76 | 207 | 388 | 521 | 1.08e-14 | 72.4 |
| MS.gene75429.t1 | MTR_8g106240 | 36.634 | 101 | 61 | 2 | 106 | 203 | 252 | 352 | 1.15e-14 | 72.0 |
| MS.gene75429.t1 | MTR_7g100950 | 39.583 | 96 | 57 | 1 | 103 | 197 | 447 | 542 | 4.60e-14 | 70.5 |
| MS.gene75429.t1 | MTR_7g100950 | 39.583 | 96 | 57 | 1 | 103 | 197 | 449 | 544 | 5.11e-14 | 70.5 |
| MS.gene75429.t1 | MTR_1g019200 | 38.710 | 93 | 55 | 1 | 107 | 197 | 609 | 701 | 1.64e-13 | 68.9 |
| MS.gene75429.t1 | MTR_1g066430 | 40.860 | 93 | 51 | 3 | 107 | 197 | 602 | 692 | 2.00e-13 | 68.9 |
| MS.gene75429.t1 | MTR_3g108808 | 33.019 | 106 | 62 | 1 | 106 | 202 | 428 | 533 | 8.12e-12 | 63.9 |
| MS.gene75429.t1 | MTR_5g016960 | 35.577 | 104 | 64 | 2 | 98 | 198 | 489 | 592 | 2.49e-11 | 62.8 |
| MS.gene75429.t1 | MTR_1g114620 | 38.095 | 105 | 61 | 3 | 104 | 207 | 213 | 314 | 3.46e-11 | 61.6 |
| MS.gene75429.t1 | MTR_5g016980 | 43.243 | 74 | 38 | 2 | 128 | 197 | 255 | 328 | 5.75e-11 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75429.t1 | AT3G47180 | 56.452 | 124 | 51 | 2 | 81 | 203 | 88 | 209 | 3.95e-36 | 126 |
| MS.gene75429.t1 | AT3G19910 | 54.000 | 100 | 44 | 2 | 105 | 203 | 236 | 334 | 8.10e-30 | 113 |
| MS.gene75429.t1 | AT3G63530 | 42.478 | 113 | 60 | 3 | 93 | 201 | 130 | 241 | 1.23e-25 | 100 |
| MS.gene75429.t1 | AT3G63530 | 42.478 | 113 | 60 | 3 | 93 | 201 | 130 | 241 | 1.23e-25 | 100 |
| MS.gene75429.t1 | AT2G15530 | 42.056 | 107 | 56 | 3 | 104 | 204 | 598 | 704 | 2.18e-18 | 83.2 |
| MS.gene75429.t1 | AT2G15530 | 42.056 | 107 | 56 | 3 | 104 | 204 | 598 | 704 | 2.18e-18 | 83.2 |
| MS.gene75429.t1 | AT2G15530 | 42.056 | 107 | 56 | 3 | 104 | 204 | 598 | 704 | 2.18e-18 | 83.2 |
| MS.gene75429.t1 | AT2G15530 | 42.056 | 107 | 56 | 3 | 104 | 204 | 598 | 704 | 2.18e-18 | 83.2 |
| MS.gene75429.t1 | AT2G15530 | 42.056 | 107 | 56 | 3 | 104 | 204 | 598 | 704 | 2.18e-18 | 83.2 |
| MS.gene75429.t1 | AT5G52140 | 39.815 | 108 | 47 | 2 | 108 | 201 | 175 | 278 | 2.52e-18 | 81.3 |
| MS.gene75429.t1 | AT2G15530 | 42.056 | 107 | 56 | 3 | 104 | 204 | 576 | 682 | 3.04e-18 | 82.8 |
| MS.gene75429.t1 | AT1G17970 | 39.837 | 123 | 64 | 4 | 78 | 197 | 245 | 360 | 8.72e-18 | 80.9 |
| MS.gene75429.t1 | AT4G34040 | 41.935 | 93 | 52 | 1 | 107 | 197 | 567 | 659 | 1.15e-17 | 81.3 |
| MS.gene75429.t1 | AT1G73760 | 36.082 | 97 | 62 | 0 | 101 | 197 | 263 | 359 | 1.61e-17 | 80.1 |
| MS.gene75429.t1 | AT2G15530 | 41.837 | 98 | 52 | 2 | 104 | 196 | 598 | 695 | 1.31e-16 | 78.2 |
| MS.gene75429.t1 | AT4G31450 | 35.714 | 112 | 61 | 1 | 106 | 206 | 386 | 497 | 1.37e-15 | 75.1 |
| MS.gene75429.t1 | AT4G31450 | 35.714 | 112 | 61 | 1 | 106 | 206 | 386 | 497 | 1.37e-15 | 75.1 |
| MS.gene75429.t1 | AT1G53190 | 35.385 | 130 | 66 | 4 | 82 | 201 | 365 | 486 | 3.06e-15 | 73.9 |
| MS.gene75429.t1 | AT1G53190 | 35.385 | 130 | 66 | 4 | 82 | 201 | 365 | 486 | 3.06e-15 | 73.9 |
| MS.gene75429.t1 | AT3G15070 | 35.246 | 122 | 70 | 2 | 94 | 209 | 362 | 480 | 9.53e-15 | 72.4 |
| MS.gene75429.t1 | AT3G15070 | 35.246 | 122 | 70 | 2 | 94 | 209 | 362 | 480 | 9.53e-15 | 72.4 |
| MS.gene75429.t1 | AT5G42940 | 40.217 | 92 | 54 | 1 | 107 | 197 | 586 | 677 | 1.77e-14 | 72.0 |
| MS.gene75429.t1 | AT5G42940 | 40.217 | 92 | 54 | 1 | 107 | 197 | 586 | 677 | 1.77e-14 | 72.0 |
| MS.gene75429.t1 | AT5G10650 | 36.364 | 99 | 56 | 1 | 106 | 197 | 417 | 515 | 2.57e-14 | 71.2 |
| MS.gene75429.t1 | AT5G10650 | 36.364 | 99 | 56 | 1 | 106 | 197 | 417 | 515 | 2.57e-14 | 71.2 |
| MS.gene75429.t1 | AT1G36950 | 30.357 | 112 | 72 | 3 | 90 | 197 | 11 | 120 | 7.89e-13 | 65.5 |
| MS.gene75429.t1 | AT2G37150 | 44.086 | 93 | 51 | 1 | 106 | 197 | 457 | 549 | 1.32e-12 | 66.2 |
| MS.gene75429.t1 | AT2G37150 | 44.086 | 93 | 51 | 1 | 106 | 197 | 457 | 549 | 1.32e-12 | 66.2 |
| MS.gene75429.t1 | AT2G37150 | 44.086 | 93 | 51 | 1 | 106 | 197 | 457 | 549 | 1.32e-12 | 66.2 |
| MS.gene75429.t1 | AT2G37150 | 44.086 | 93 | 51 | 1 | 106 | 197 | 441 | 533 | 1.39e-12 | 66.2 |
| MS.gene75429.t1 | AT2G37150 | 44.086 | 93 | 51 | 1 | 106 | 197 | 441 | 533 | 1.39e-12 | 66.2 |
| MS.gene75429.t1 | AT2G37150 | 44.086 | 93 | 51 | 1 | 106 | 197 | 441 | 533 | 1.39e-12 | 66.2 |
| MS.gene75429.t1 | AT2G37150 | 44.086 | 93 | 51 | 1 | 106 | 197 | 441 | 533 | 1.39e-12 | 66.2 |
| MS.gene75429.t1 | AT2G37150 | 44.086 | 93 | 51 | 1 | 106 | 197 | 441 | 533 | 1.39e-12 | 66.2 |
| MS.gene75429.t1 | AT2G37150 | 44.086 | 93 | 51 | 1 | 106 | 197 | 441 | 533 | 1.39e-12 | 66.2 |
| MS.gene75429.t1 | AT2G37150 | 44.086 | 93 | 51 | 1 | 106 | 197 | 441 | 533 | 1.39e-12 | 66.2 |
| MS.gene75429.t1 | AT2G37150 | 44.086 | 93 | 51 | 1 | 106 | 197 | 441 | 533 | 1.39e-12 | 66.2 |
| MS.gene75429.t1 | AT2G37150 | 44.086 | 93 | 51 | 1 | 106 | 197 | 441 | 533 | 1.39e-12 | 66.2 |
| MS.gene75429.t1 | AT2G37150 | 44.086 | 93 | 51 | 1 | 106 | 197 | 441 | 533 | 1.39e-12 | 66.2 |
| MS.gene75429.t1 | AT2G37150 | 44.086 | 93 | 51 | 1 | 106 | 197 | 441 | 533 | 1.39e-12 | 66.2 |
| MS.gene75429.t1 | AT2G37150 | 44.086 | 93 | 51 | 1 | 106 | 197 | 494 | 586 | 1.63e-12 | 66.2 |
| MS.gene75429.t1 | AT5G67120 | 36.170 | 94 | 55 | 2 | 110 | 201 | 181 | 271 | 4.33e-12 | 63.9 |
| MS.gene75429.t1 | AT1G45180 | 37.143 | 105 | 64 | 2 | 107 | 209 | 541 | 645 | 4.54e-12 | 64.7 |
| MS.gene75429.t1 | AT1G45180 | 37.143 | 105 | 64 | 2 | 107 | 209 | 541 | 645 | 4.54e-12 | 64.7 |
| MS.gene75429.t1 | AT1G45180 | 37.143 | 105 | 64 | 2 | 107 | 209 | 541 | 645 | 4.54e-12 | 64.7 |
| MS.gene75429.t1 | AT1G45180 | 36.792 | 106 | 65 | 2 | 106 | 209 | 492 | 597 | 6.13e-11 | 61.6 |
Find 29 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACGAAGAGTTAATTGAATT+AGG | 0.293489 | 3.3:+73330348 | MS.gene75429:CDS |
| TTCATCACTTTCACTTTCAA+TGG | 0.333913 | 3.3:-73330145 | None:intergenic |
| GTGTTCTTAGCCTCCTTGTT+GGG | 0.365855 | 3.3:-73330618 | None:intergenic |
| TGCTGATTTCTCATTTAGTC+TGG | 0.368295 | 3.3:+73330209 | MS.gene75429:CDS |
| GATACTTCATTGCTACAGAT+TGG | 0.416227 | 3.3:-73330591 | None:intergenic |
| ACTTCCTCAAGTTCAGTGTA+TGG | 0.418215 | 3.3:-73330048 | None:intergenic |
| TCTTCGTAAGATAATTCATC+TGG | 0.426081 | 3.3:-73330333 | None:intergenic |
| CTTCAGTTTCTTGAAGATGA+AGG | 0.444544 | 3.3:+73330246 | MS.gene75429:CDS |
| CAAGTGGCTTCAAATTAAGA+AGG | 0.448967 | 3.3:+73330563 | MS.gene75429:CDS |
| CTTATACAATCTGTATGATA+AGG | 0.449658 | 3.3:-73330540 | None:intergenic |
| TGATTATGATGACGATGAAA+TGG | 0.456319 | 3.3:+73330287 | MS.gene75429:CDS |
| ATTGAATTAGGAGACTTCAT+AGG | 0.461812 | 3.3:+73330360 | MS.gene75429:CDS |
| CAAGAAGAGATTTCATTAGC+TGG | 0.470448 | 3.3:-73330402 | None:intergenic |
| AATATCATCATTACTAAAAG+AGG | 0.473327 | 3.3:-73330181 | None:intergenic |
| TGTCAGATTGAATATGAAGA+AGG | 0.485591 | 3.3:+73330486 | MS.gene75429:CDS |
| AGTGTTCTTAGCCTCCTTGT+TGG | 0.513411 | 3.3:-73330619 | None:intergenic |
| ATCTTCAAGAAACTGAAGCT+CGG | 0.528667 | 3.3:-73330241 | None:intergenic |
| TGATGACGATGAAATGGAGT+TGG | 0.538895 | 3.3:+73330293 | MS.gene75429:CDS |
| TACAATCTGTATGATAAGGA+TGG | 0.563521 | 3.3:-73330536 | None:intergenic |
| TTCTTTAATGGAACGCGAAG+AGG | 0.577942 | 3.3:+73330002 | None:intergenic |
| TCTTTAATGGAACGCGAAGA+GGG | 0.582041 | 3.3:+73330003 | None:intergenic |
| AAATCCATACACTGAACTTG+AGG | 0.582977 | 3.3:+73330044 | MS.gene75429:CDS |
| ATATGAAGAAGGTGAAGCAT+TGG | 0.600446 | 3.3:+73330497 | MS.gene75429:CDS |
| ATACAGATTGTATAAGCAAG+TGG | 0.600932 | 3.3:+73330547 | MS.gene75429:CDS |
| AAAATTGCTGAAAGCACAAG+TGG | 0.605945 | 3.3:+73330444 | MS.gene75429:CDS |
| TGAAGTATCAACTCCCAACA+AGG | 0.639111 | 3.3:+73330605 | MS.gene75429:CDS |
| AATGGAGTTGGAAGAAGATG+AGG | 0.656996 | 3.3:+73330305 | MS.gene75429:CDS |
| TTCATAGGAGAAGAGAAGAG+AGG | 0.670361 | 3.3:+73330375 | MS.gene75429:CDS |
| AGTATCAACTCCCAACAAGG+AGG | 0.732320 | 3.3:+73330608 | MS.gene75429:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATATCATCATTACTAAAAG+AGG | - | chr3.3:73330184-73330203 | None:intergenic | 20.0% |
| !!! | TCTTTTAGTAATGATGATAT+TGG | + | chr3.3:73330183-73330202 | MS.gene75429:CDS | 20.0% |
| ! | CTTATACAATCTGTATGATA+AGG | - | chr3.3:73330543-73330562 | None:intergenic | 25.0% |
| ! | TACGAAGAGTTAATTGAATT+AGG | + | chr3.3:73330348-73330367 | MS.gene75429:CDS | 25.0% |
| !!! | TTCAGTGTATGGATTTTTTT+CGG | - | chr3.3:73330040-73330059 | None:intergenic | 25.0% |
| ATACAGATTGTATAAGCAAG+TGG | + | chr3.3:73330547-73330566 | MS.gene75429:CDS | 30.0% | |
| ATTGAATTAGGAGACTTCAT+AGG | + | chr3.3:73330360-73330379 | MS.gene75429:CDS | 30.0% | |
| TACAATCTGTATGATAAGGA+TGG | - | chr3.3:73330539-73330558 | None:intergenic | 30.0% | |
| TCTTCGTAAGATAATTCATC+TGG | - | chr3.3:73330336-73330355 | None:intergenic | 30.0% | |
| TGTCAGATTGAATATGAAGA+AGG | + | chr3.3:73330486-73330505 | MS.gene75429:CDS | 30.0% | |
| TTCATCACTTTCACTTTCAA+TGG | - | chr3.3:73330148-73330167 | None:intergenic | 30.0% | |
| ! | CAGCAATTTTGCAAGTATAA+GGG | - | chr3.3:73330434-73330453 | None:intergenic | 30.0% |
| ! | TCAGCAATTTTGCAAGTATA+AGG | - | chr3.3:73330435-73330454 | None:intergenic | 30.0% |
| ! | TCTGATTTTGTTCTAGCAAT+TGG | + | chr3.3:73330075-73330094 | MS.gene75429:CDS | 30.0% |
| ! | TGATTATGATGACGATGAAA+TGG | + | chr3.3:73330287-73330306 | MS.gene75429:CDS | 30.0% |
| AAATCCATACACTGAACTTG+AGG | + | chr3.3:73330044-73330063 | MS.gene75429:CDS | 35.0% | |
| ATATGAAGAAGGTGAAGCAT+TGG | + | chr3.3:73330497-73330516 | MS.gene75429:CDS | 35.0% | |
| ATCTTCAAGAAACTGAAGCT+CGG | - | chr3.3:73330244-73330263 | None:intergenic | 35.0% | |
| CAAGAAGAGATTTCATTAGC+TGG | - | chr3.3:73330405-73330424 | None:intergenic | 35.0% | |
| CAAGTGGCTTCAAATTAAGA+AGG | + | chr3.3:73330563-73330582 | MS.gene75429:CDS | 35.0% | |
| CTTCAGTTTCTTGAAGATGA+AGG | + | chr3.3:73330246-73330265 | MS.gene75429:CDS | 35.0% | |
| GATACTTCATTGCTACAGAT+TGG | - | chr3.3:73330594-73330613 | None:intergenic | 35.0% | |
| ! | AAAATTGCTGAAAGCACAAG+TGG | + | chr3.3:73330444-73330463 | MS.gene75429:CDS | 35.0% |
| !! | TGCTGATTTCTCATTTAGTC+TGG | + | chr3.3:73330209-73330228 | MS.gene75429:CDS | 35.0% |
| AATGGAGTTGGAAGAAGATG+AGG | + | chr3.3:73330305-73330324 | MS.gene75429:CDS | 40.0% | |
| ACTTCCTCAAGTTCAGTGTA+TGG | - | chr3.3:73330051-73330070 | None:intergenic | 40.0% | |
| TTCATAGGAGAAGAGAAGAG+AGG | + | chr3.3:73330375-73330394 | MS.gene75429:CDS | 40.0% | |
| ! | TGAAGTATCAACTCCCAACA+AGG | + | chr3.3:73330605-73330624 | MS.gene75429:CDS | 40.0% |
| ! | TGATGACGATGAAATGGAGT+TGG | + | chr3.3:73330293-73330312 | MS.gene75429:CDS | 40.0% |
| AGTATCAACTCCCAACAAGG+AGG | + | chr3.3:73330608-73330627 | MS.gene75429:CDS | 45.0% | |
| AGTGTTCTTAGCCTCCTTGT+TGG | - | chr3.3:73330622-73330641 | None:intergenic | 45.0% | |
| GTGTTCTTAGCCTCCTTGTT+GGG | - | chr3.3:73330621-73330640 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 73330009 | 73330644 | 73330009 | ID=MS.gene75429 |
| chr3.3 | mRNA | 73330009 | 73330644 | 73330009 | ID=MS.gene75429.t1;Parent=MS.gene75429 |
| chr3.3 | exon | 73330009 | 73330644 | 73330009 | ID=MS.gene75429.t1.exon1;Parent=MS.gene75429.t1 |
| chr3.3 | CDS | 73330009 | 73330644 | 73330009 | ID=cds.MS.gene75429.t1;Parent=MS.gene75429.t1 |
| Gene Sequence |
| Protein sequence |
