Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75454.t1 | XP_003601999.1 | 73.7 | 190 | 39 | 3 | 1 | 189 | 18 | 197 | 3.30E-65 | 258.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75454.t1 | Q9SGZ8 | 54.8 | 73 | 33 | 0 | 115 | 187 | 88 | 160 | 3.8e-14 | 79.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75454.t1 | G7J4C7 | 73.7 | 190 | 39 | 3 | 1 | 189 | 18 | 197 | 2.3e-65 | 258.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053169 | MS.gene75454 | 0.813806 | 2.12E-51 | -1.69E-46 |
| MS.gene054556 | MS.gene75454 | 0.81935 | 1.22E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75454.t1 | MTR_3g087700 | 87.006 | 177 | 12 | 3 | 1 | 176 | 18 | 184 | 8.49e-111 | 314 |
| MS.gene75454.t1 | MTR_3g087700 | 83.942 | 137 | 11 | 3 | 1 | 136 | 18 | 144 | 1.63e-77 | 228 |
| MS.gene75454.t1 | MTR_3g073960 | 59.322 | 59 | 23 | 1 | 102 | 160 | 77 | 134 | 5.71e-20 | 82.4 |
| MS.gene75454.t1 | MTR_8g008840 | 53.571 | 56 | 25 | 1 | 110 | 164 | 32 | 87 | 1.72e-15 | 69.7 |
| MS.gene75454.t1 | MTR_4g099050 | 61.702 | 47 | 18 | 0 | 114 | 160 | 71 | 117 | 5.03e-15 | 69.3 |
| MS.gene75454.t1 | MTR_5g013770 | 50.704 | 71 | 29 | 2 | 114 | 179 | 83 | 152 | 9.92e-15 | 68.9 |
| MS.gene75454.t1 | MTR_8g022950 | 44.286 | 70 | 36 | 1 | 116 | 185 | 39 | 105 | 7.47e-14 | 65.5 |
| MS.gene75454.t1 | MTR_2g436020 | 58.537 | 41 | 17 | 0 | 116 | 156 | 64 | 104 | 7.85e-12 | 60.5 |
| MS.gene75454.t1 | MTR_2g436250 | 58.537 | 41 | 17 | 0 | 116 | 156 | 64 | 104 | 7.85e-12 | 60.5 |
| MS.gene75454.t1 | MTR_4g124930 | 54.902 | 51 | 22 | 1 | 110 | 160 | 172 | 221 | 8.98e-12 | 62.4 |
| MS.gene75454.t1 | MTR_4g124930 | 54.902 | 51 | 22 | 1 | 110 | 160 | 172 | 221 | 9.13e-12 | 62.0 |
| MS.gene75454.t1 | MTR_2g042970 | 40.741 | 81 | 45 | 2 | 84 | 162 | 152 | 231 | 1.08e-11 | 62.0 |
| MS.gene75454.t1 | MTR_4g120160 | 45.000 | 60 | 32 | 1 | 97 | 156 | 44 | 102 | 4.36e-11 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75454.t1 | AT1G78020 | 53.333 | 75 | 35 | 0 | 115 | 189 | 88 | 162 | 1.22e-21 | 87.0 |
| MS.gene75454.t1 | AT1G22160 | 70.213 | 47 | 14 | 0 | 116 | 162 | 78 | 124 | 1.01e-17 | 76.3 |
| MS.gene75454.t1 | AT4G39795 | 45.833 | 72 | 36 | 1 | 95 | 166 | 55 | 123 | 3.51e-17 | 74.3 |
| MS.gene75454.t1 | AT4G17670 | 62.000 | 50 | 19 | 0 | 111 | 160 | 71 | 120 | 7.45e-17 | 74.3 |
| MS.gene75454.t1 | AT5G65040 | 51.389 | 72 | 31 | 1 | 97 | 168 | 42 | 109 | 1.26e-16 | 72.4 |
| MS.gene75454.t1 | AT2G44670 | 62.000 | 50 | 18 | 1 | 114 | 162 | 14 | 63 | 5.59e-16 | 70.1 |
| MS.gene75454.t1 | AT5G47060 | 63.830 | 47 | 17 | 0 | 114 | 160 | 95 | 141 | 3.88e-15 | 70.1 |
| MS.gene75454.t1 | AT5G47060 | 63.830 | 47 | 17 | 0 | 114 | 160 | 95 | 141 | 3.88e-15 | 70.1 |
| MS.gene75454.t1 | AT1G53885 | 48.529 | 68 | 33 | 1 | 92 | 159 | 20 | 85 | 1.43e-12 | 62.0 |
| MS.gene75454.t1 | AT1G53903 | 48.529 | 68 | 33 | 1 | 92 | 159 | 20 | 85 | 1.43e-12 | 62.0 |
| MS.gene75454.t1 | AT1G19200 | 49.091 | 55 | 27 | 1 | 109 | 163 | 143 | 196 | 3.92e-11 | 60.1 |
| MS.gene75454.t1 | AT5G49120 | 39.130 | 69 | 42 | 0 | 88 | 156 | 40 | 108 | 3.94e-11 | 58.9 |
| MS.gene75454.t1 | AT1G19200 | 49.091 | 55 | 27 | 1 | 109 | 163 | 156 | 209 | 4.60e-11 | 60.1 |
| MS.gene75454.t1 | AT1G74940 | 45.902 | 61 | 32 | 1 | 115 | 175 | 149 | 208 | 5.82e-11 | 59.7 |
Find 46 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCCTCATGATGGGATTCT+AGG | 0.211376 | 3.3:+73043979 | None:intergenic |
| AATAATATTGTTAGTGTTTG+TGG | 0.326456 | 3.3:+73043767 | None:intergenic |
| AGTTACAGAGGCCACAAGTT+TGG | 0.349033 | 3.3:+73043679 | None:intergenic |
| ATGATGGGATTCTAGGTTGT+TGG | 0.366587 | 3.3:+73043986 | None:intergenic |
| ACAAGCAGCAGTAGAAGCTT+TGG | 0.372677 | 3.3:+73043044 | None:intergenic |
| TCTAGGTTGTTGGTTTGATC+AGG | 0.381479 | 3.3:+73043996 | None:intergenic |
| ATTGTTAGTGTTTGTGGCTG+TGG | 0.394454 | 3.3:+73043773 | None:intergenic |
| TGTAACTGTCGTTTGGCTCC+TGG | 0.404985 | 3.3:-73043663 | MS.gene75454:CDS |
| TACACGGTGGTCTTCTTTGT+TGG | 0.433148 | 3.3:+73043089 | None:intergenic |
| TGGCCTCTGTAACTGTCGTT+TGG | 0.455985 | 3.3:-73043670 | MS.gene75454:CDS |
| CACTGATTCTTCCTCATGAT+GGG | 0.470672 | 3.3:+73043971 | None:intergenic |
| GATGGCTGATGAACGTTTAG+TGG | 0.489958 | 3.3:-73043826 | MS.gene75454:CDS |
| TCACTGATTCTTCCTCATGA+TGG | 0.491322 | 3.3:+73043970 | None:intergenic |
| AAGACAAAAGAAAAGAAAAG+TGG | 0.499141 | 3.3:-73043125 | MS.gene75454:CDS |
| GTTCCCATCACAACCGTATG+TGG | 0.501901 | 3.3:+73043876 | None:intergenic |
| GAATCCACATACGGTTGTGA+TGG | 0.509624 | 3.3:-73043880 | MS.gene75454:CDS |
| TGATCATCATGGTGTGGATG+TGG | 0.510086 | 3.3:+73043924 | None:intergenic |
| GTGGCTGTGGCTGTGTGTGA+AGG | 0.511685 | 3.3:+73043786 | None:intergenic |
| GTCGAGACATCTACATGTAT+AGG | 0.522956 | 3.3:-73043641 | MS.gene75454:intron |
| ATCAGGTGGTTGCATGTCCA+CGG | 0.524115 | 3.3:+73044013 | None:intergenic |
| AAGAAAAGAAAAGTGGAAGA+TGG | 0.529987 | 3.3:-73043118 | MS.gene75454:CDS |
| AGGTTGTTGGTTTGATCAGG+TGG | 0.542107 | 3.3:+73043999 | None:intergenic |
| TGGCTGTGGCTGTGTGTGAA+GGG | 0.543537 | 3.3:+73043787 | None:intergenic |
| TGCCATGTTCTTGATCATCA+TGG | 0.545211 | 3.3:+73043913 | None:intergenic |
| AGCAGTAGAAGCTTTGGCAG+TGG | 0.548192 | 3.3:+73043050 | None:intergenic |
| GCTTTGGCAGTGGCCGTGCA+CGG | 0.562038 | 3.3:+73043060 | None:intergenic |
| TCATGTGCACTCACACTCAA+AGG | 0.576185 | 3.3:+73043729 | None:intergenic |
| CCGTGTATCACCACCGTGCA+CGG | 0.577796 | 3.3:-73043073 | MS.gene75454:CDS |
| AATCCACATACGGTTGTGAT+GGG | 0.578470 | 3.3:-73043879 | MS.gene75454:CDS |
| CCTCACTTTCTCCAAACTTG+TGG | 0.579241 | 3.3:-73043690 | MS.gene75454:CDS |
| CCGTGCACGGTGGTGATACA+CGG | 0.602872 | 3.3:+73043073 | None:intergenic |
| CACCATGATGATCAAGAACA+TGG | 0.605152 | 3.3:-73043915 | MS.gene75454:CDS |
| TGGTGTGGATGTGGCTGCAG+AGG | 0.618105 | 3.3:+73043933 | None:intergenic |
| ATGGCTGATGAACGTTTAGT+GGG | 0.621978 | 3.3:-73043825 | MS.gene75454:CDS |
| CCACAAGTTTGGAGAAAGTG+AGG | 0.629285 | 3.3:+73043690 | None:intergenic |
| TGTTCTTGATCATCATGGTG+TGG | 0.629577 | 3.3:+73043918 | None:intergenic |
| CAACATCAAGAATCCACATA+CGG | 0.631823 | 3.3:-73043889 | MS.gene75454:CDS |
| CATGAGTGGTGGTTTGTCCG+TGG | 0.637137 | 3.3:-73044030 | MS.gene75454:CDS |
| AGAAGAACAAGAAGCATGAG+TGG | 0.643370 | 3.3:-73044044 | None:intergenic |
| TACATGTAGATGTCTCGACC+AGG | 0.645212 | 3.3:+73043645 | None:intergenic |
| CGTTAAAAGTGAATTAACGA+TGG | 0.660105 | 3.3:-73043844 | MS.gene75454:CDS |
| CAACCTAGAATCCCATCATG+AGG | 0.662994 | 3.3:-73043982 | MS.gene75454:CDS |
| TTGGCAGTGGCCGTGCACGG+TGG | 0.682224 | 3.3:+73043063 | None:intergenic |
| GAGCCAAACGACAGTTACAG+AGG | 0.685876 | 3.3:+73043667 | None:intergenic |
| AGAACAAGAAGCATGAGTGG+TGG | 0.691730 | 3.3:-73044041 | None:intergenic |
| TGCACGGTGGTGATACACGG+TGG | 0.754526 | 3.3:+73043076 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATGAATAATAGAAAAAAA+TGG | + | chr3.3:73043459-73043478 | None:intergenic | 10.0% |
| !!! | TTAATTAATTTTTTGTTTAG+AGG | - | chr3.3:73043796-73043815 | MS.gene75454:CDS | 10.0% |
| !! | GTTAGTTAGTATAATTAATT+TGG | - | chr3.3:73043827-73043846 | MS.gene75454:CDS | 15.0% |
| !!! | AAAGAAAATTATTGATTTCA+GGG | - | chr3.3:73043871-73043890 | MS.gene75454:CDS | 15.0% |
| !! | AATAATATTGTTAGTGTTTG+TGG | + | chr3.3:73043303-73043322 | None:intergenic | 20.0% |
| !! | GTAAATATTAATTCTTCCTT+TGG | + | chr3.3:73043686-73043705 | None:intergenic | 20.0% |
| !!! | AAGAAAATTATTGATTTCAG+GGG | - | chr3.3:73043872-73043891 | MS.gene75454:CDS | 20.0% |
| !!! | GAAAGAAAATTATTGATTTC+AGG | - | chr3.3:73043870-73043889 | MS.gene75454:CDS | 20.0% |
| ! | AAGACAAAAGAAAAGAAAAG+TGG | - | chr3.3:73043942-73043961 | MS.gene75454:CDS | 25.0% |
| ! | CTGAAAATAAAGAGACAATT+AGG | + | chr3.3:73043656-73043675 | None:intergenic | 25.0% |
| ! | TAAACAAGAATATCAAAGTC+AGG | - | chr3.3:73043566-73043585 | MS.gene75454:intron | 25.0% |
| !! | TGACTTTGATATTCTTGTTT+AGG | + | chr3.3:73043567-73043586 | None:intergenic | 25.0% |
| !!! | TTTTTGTTTAGAGGAAATTG+TGG | - | chr3.3:73043805-73043824 | MS.gene75454:CDS | 25.0% |
| AAGAAAAGAAAAGTGGAAGA+TGG | - | chr3.3:73043949-73043968 | MS.gene75454:CDS | 30.0% | |
| ACTAACCATAAGAGTTAGTT+AGG | - | chr3.3:73043593-73043612 | MS.gene75454:intron | 30.0% | |
| CGTTAAAAGTGAATTAACGA+TGG | - | chr3.3:73043223-73043242 | MS.gene75454:intron | 30.0% | |
| TTCATTCTAGTAGTTTGACT+AGG | - | chr3.3:73043715-73043734 | MS.gene75454:CDS | 30.0% | |
| TTGAACCTAACTAACTCTTA+TGG | + | chr3.3:73043601-73043620 | None:intergenic | 30.0% | |
| !! | TATTTTCAGTATCGAACCAA+AGG | - | chr3.3:73043667-73043686 | MS.gene75454:CDS | 30.0% |
| AGACATCTACATGTATAGGT+AGG | - | chr3.3:73043430-73043449 | MS.gene75454:intron | 35.0% | |
| CAACATCAAGAATCCACATA+CGG | - | chr3.3:73043178-73043197 | MS.gene75454:CDS | 35.0% | |
| ! | AGATACAGCATTTTGCAGTT+TGG | - | chr3.3:73043895-73043914 | MS.gene75454:CDS | 35.0% |
| ! | TTTTACCTTTGACTGAACTC+CGG | + | chr3.3:73043540-73043559 | None:intergenic | 35.0% |
| AATCCACATACGGTTGTGAT+GGG | - | chr3.3:73043188-73043207 | MS.gene75454:intron | 40.0% | |
| ATGGCTGATGAACGTTTAGT+GGG | - | chr3.3:73043242-73043261 | MS.gene75454:intron | 40.0% | |
| ATTGTTAGTGTTTGTGGCTG+TGG | + | chr3.3:73043297-73043316 | None:intergenic | 40.0% | |
| CACCATGATGATCAAGAACA+TGG | - | chr3.3:73043152-73043171 | MS.gene75454:CDS | 40.0% | |
| GTCGAGACATCTACATGTAT+AGG | - | chr3.3:73043426-73043445 | MS.gene75454:intron | 40.0% | |
| TGCCATGTTCTTGATCATCA+TGG | + | chr3.3:73043157-73043176 | None:intergenic | 40.0% | |
| TGTTCTTGATCATCATGGTG+TGG | + | chr3.3:73043152-73043171 | None:intergenic | 40.0% | |
| ! | ATGATGGGATTCTAGGTTGT+TGG | + | chr3.3:73043084-73043103 | None:intergenic | 40.0% |
| !! | ATTTTGCAGTTTGGAGTGCA+GGG | - | chr3.3:73043904-73043923 | MS.gene75454:CDS | 40.0% |
| !! | CACTGATTCTTCCTCATGAT+GGG | + | chr3.3:73043099-73043118 | None:intergenic | 40.0% |
| !! | TCACTGATTCTTCCTCATGA+TGG | + | chr3.3:73043100-73043119 | None:intergenic | 40.0% |
| !! | TCTAGGTTGTTGGTTTGATC+AGG | + | chr3.3:73043074-73043093 | None:intergenic | 40.0% |
| ACAAGCAGCAGTAGAAGCTT+TGG | + | chr3.3:73044026-73044045 | None:intergenic | 45.0% | |
| AGTTACAGAGGCCACAAGTT+TGG | + | chr3.3:73043391-73043410 | None:intergenic | 45.0% | |
| CAACCTAGAATCCCATCATG+AGG | - | chr3.3:73043085-73043104 | MS.gene75454:CDS | 45.0% | |
| CCACAAGTTTGGAGAAAGTG+AGG | + | chr3.3:73043380-73043399 | None:intergenic | 45.0% | |
| CCTCACTTTCTCCAAACTTG+TGG | - | chr3.3:73043377-73043396 | MS.gene75454:intron | 45.0% | |
| GAAATCCGGAGTTCAGTCAA+AGG | - | chr3.3:73043532-73043551 | MS.gene75454:intron | 45.0% | |
| GAATCCACATACGGTTGTGA+TGG | - | chr3.3:73043187-73043206 | MS.gene75454:intron | 45.0% | |
| GATGGCTGATGAACGTTTAG+TGG | - | chr3.3:73043241-73043260 | MS.gene75454:intron | 45.0% | |
| TACACGGTGGTCTTCTTTGT+TGG | + | chr3.3:73043981-73044000 | None:intergenic | 45.0% | |
| TACATGTAGATGTCTCGACC+AGG | + | chr3.3:73043425-73043444 | None:intergenic | 45.0% | |
| TCATGTGCACTCACACTCAA+AGG | + | chr3.3:73043341-73043360 | None:intergenic | 45.0% | |
| TGATCATCATGGTGTGGATG+TGG | + | chr3.3:73043146-73043165 | None:intergenic | 45.0% | |
| TGTAAACCCTACTCTGCCTA+GGG | - | chr3.3:73043494-73043513 | MS.gene75454:intron | 45.0% | |
| ! | CTTCCTCATGATGGGATTCT+AGG | + | chr3.3:73043091-73043110 | None:intergenic | 45.0% |
| !! | AGGTTGTTGGTTTGATCAGG+TGG | + | chr3.3:73043071-73043090 | None:intergenic | 45.0% |
| !! | CATTTTGCAGTTTGGAGTGC+AGG | - | chr3.3:73043903-73043922 | MS.gene75454:CDS | 45.0% |
| AAGGCAGAGCCTTCGAAATC+CGG | - | chr3.3:73043518-73043537 | MS.gene75454:intron | 50.0% | |
| ATCAGGTGGTTGCATGTCCA+CGG | + | chr3.3:73043057-73043076 | None:intergenic | 50.0% | |
| GACTGAACTCCGGATTTCGA+AGG | + | chr3.3:73043530-73043549 | None:intergenic | 50.0% | |
| GAGCCAAACGACAGTTACAG+AGG | + | chr3.3:73043403-73043422 | None:intergenic | 50.0% | |
| GTGTAAACCCTACTCTGCCT+AGG | - | chr3.3:73043493-73043512 | MS.gene75454:intron | 50.0% | |
| GTTCCCATCACAACCGTATG+TGG | + | chr3.3:73043194-73043213 | None:intergenic | 50.0% | |
| TGGCCTCTGTAACTGTCGTT+TGG | - | chr3.3:73043397-73043416 | MS.gene75454:intron | 50.0% | |
| ! | AGCAGTAGAAGCTTTGGCAG+TGG | + | chr3.3:73044020-73044039 | None:intergenic | 50.0% |
| ! | TGTAACTGTCGTTTGGCTCC+TGG | - | chr3.3:73043404-73043423 | MS.gene75454:intron | 50.0% |
| TGGCTGTGGCTGTGTGTGAA+GGG | + | chr3.3:73043283-73043302 | None:intergenic | 55.0% | |
| ACCCTACTCTGCCTAGGGCA+AGG | - | chr3.3:73043499-73043518 | MS.gene75454:intron | 60.0% | |
| CCGTGCACGGTGGTGATACA+CGG | + | chr3.3:73043997-73044016 | None:intergenic | 60.0% | |
| CCGTGTATCACCACCGTGCA+CGG | - | chr3.3:73043994-73044013 | MS.gene75454:CDS | 60.0% | |
| GCCTTGCCCTAGGCAGAGTA+GGG | + | chr3.3:73043503-73043522 | None:intergenic | 60.0% | |
| GTGGCTGTGGCTGTGTGTGA+AGG | + | chr3.3:73043284-73043303 | None:intergenic | 60.0% | |
| TGCACGGTGGTGATACACGG+TGG | + | chr3.3:73043994-73044013 | None:intergenic | 60.0% | |
| TGCCTTGCCCTAGGCAGAGT+AGG | + | chr3.3:73043504-73043523 | None:intergenic | 60.0% | |
| TGGTGTGGATGTGGCTGCAG+AGG | + | chr3.3:73043137-73043156 | None:intergenic | 60.0% | |
| CGAAGGCTCTGCCTTGCCCT+AGG | + | chr3.3:73043513-73043532 | None:intergenic | 65.0% | |
| GCTTTGGCAGTGGCCGTGCA+CGG | + | chr3.3:73044010-73044029 | None:intergenic | 65.0% | |
| TTGGCAGTGGCCGTGCACGG+TGG | + | chr3.3:73044007-73044026 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 73043038 | 73044051 | 73043038 | ID=MS.gene75454 |
| chr3.3 | mRNA | 73043038 | 73044051 | 73043038 | ID=MS.gene75454.t1;Parent=MS.gene75454 |
| chr3.3 | exon | 73043642 | 73044051 | 73043642 | ID=MS.gene75454.t1.exon1;Parent=MS.gene75454.t1 |
| chr3.3 | CDS | 73043642 | 73044051 | 73043642 | ID=cds.MS.gene75454.t1;Parent=MS.gene75454.t1 |
| chr3.3 | exon | 73043038 | 73043197 | 73043038 | ID=MS.gene75454.t1.exon2;Parent=MS.gene75454.t1 |
| chr3.3 | CDS | 73043038 | 73043197 | 73043038 | ID=cds.MS.gene75454.t1;Parent=MS.gene75454.t1 |
| Gene Sequence |
| Protein sequence |
