Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75495.t1 | XP_003601934.2 | 97.5 | 394 | 10 | 0 | 1 | 394 | 1 | 394 | 6.90E-219 | 769.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75495.t1 | G7J3Q0 | 97.5 | 394 | 10 | 0 | 1 | 394 | 1 | 394 | 5.0e-219 | 769.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051860 | MS.gene75495 | 0.80759 | 4.69E-50 | -1.69E-46 |
| MS.gene055356 | MS.gene75495 | 0.82135 | 4.23E-53 | -1.69E-46 |
| MS.gene055358 | MS.gene75495 | 0.825544 | 4.43E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75495.t1 | MTR_3g087010 | 97.462 | 394 | 10 | 0 | 1 | 394 | 1 | 394 | 0.0 | 794 |
| MS.gene75495.t1 | MTR_3g087010 | 97.279 | 294 | 8 | 0 | 1 | 294 | 1 | 294 | 0.0 | 590 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75495.t1 | AT5G10320 | 53.960 | 404 | 165 | 5 | 1 | 387 | 1 | 400 | 1.01e-148 | 427 |
| MS.gene75495.t1 | AT5G10320 | 54.208 | 404 | 163 | 6 | 1 | 387 | 1 | 399 | 2.44e-148 | 426 |
| MS.gene75495.t1 | AT5G10320 | 61.111 | 288 | 98 | 2 | 110 | 387 | 12 | 295 | 2.96e-127 | 369 |
| MS.gene75495.t1 | AT5G10320 | 50.476 | 315 | 135 | 5 | 1 | 298 | 1 | 311 | 1.94e-100 | 301 |
| MS.gene75495.t1 | AT5G10320 | 50.794 | 315 | 133 | 6 | 1 | 298 | 1 | 310 | 3.31e-100 | 301 |
| MS.gene75495.t1 | AT5G10320 | 50.482 | 311 | 132 | 6 | 1 | 294 | 1 | 306 | 1.66e-97 | 293 |
Find 72 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTAGAAAGCAACACAAAATT+TGG | 0.212432 | 3.3:+72548016 | MS.gene75495:intron |
| CGCATGTTTATCAGAATATA+TGG | 0.213889 | 3.3:+72549507 | MS.gene75495:CDS |
| ATCACCTCATCTTTCCTTGT+TGG | 0.304631 | 3.3:-72547916 | None:intergenic |
| CACAGAGAGCTATATTTATC+AGG | 0.306293 | 3.3:+72546866 | MS.gene75495:CDS |
| TCTTTCAATTTCAGAGTTCT+TGG | 0.308869 | 3.3:+72548632 | MS.gene75495:CDS |
| ACAACGCATGGTTTCATTCA+TGG | 0.325233 | 3.3:+72544027 | MS.gene75495:CDS |
| AATGGAAGAGCTACGGAAAC+TGG | 0.335201 | 3.3:+72543997 | None:intergenic |
| CACAAGTCTACTGGAATTAC+TGG | 0.342434 | 3.3:+72551925 | MS.gene75495:CDS |
| CATGACAGAGCTTTCTTTCA+AGG | 0.348227 | 3.3:-72548243 | None:intergenic |
| TGTTTCTCGACTGACCCATT+TGG | 0.398597 | 3.3:+72548044 | MS.gene75495:CDS |
| GGTTTCGAACAAAATCTTGT+TGG | 0.406007 | 3.3:+72546084 | MS.gene75495:intron |
| TAGACACGGGGAAGAGTATT+GGG | 0.416091 | 3.3:+72548219 | MS.gene75495:CDS |
| GTTTGACATACCAACATAGA+AGG | 0.422519 | 3.3:-72549543 | None:intergenic |
| ATAGTCACAAGTGGTCCAAA+TGG | 0.426598 | 3.3:-72548059 | None:intergenic |
| ATTACCTTCTTCAGTGGCTT+CGG | 0.429878 | 3.3:-72551984 | None:intergenic |
| GAAGGCCGAGGAGTTGCATA+TGG | 0.458790 | 3.3:+72548605 | MS.gene75495:intron |
| CGGGCTGGATTCCATGCAAA+AGG | 0.483105 | 3.3:+72546182 | MS.gene75495:CDS |
| TTCAACTGCCCCTTCTATGT+TGG | 0.484221 | 3.3:+72549533 | MS.gene75495:CDS |
| CTCTCTGTGAATGAAAGAAT+AGG | 0.489105 | 3.3:-72546852 | None:intergenic |
| ATGAAAACATAGCCATGATC+GGG | 0.494602 | 3.3:+72546163 | MS.gene75495:CDS |
| TCCTCATCTTGAATCACAGT+TGG | 0.495108 | 3.3:-72552118 | None:intergenic |
| CATGGAATCCAGCCCGATCA+TGG | 0.498561 | 3.3:-72546175 | None:intergenic |
| TTAGACACGGGGAAGAGTAT+TGG | 0.501793 | 3.3:+72548218 | MS.gene75495:CDS |
| CTATCAACTGCAAGGAGCTA+AGG | 0.502607 | 3.3:+72548466 | MS.gene75495:CDS |
| AAGCAAGTACACAAGTCTAC+TGG | 0.503759 | 3.3:+72551916 | MS.gene75495:CDS |
| TTCAATTTCAGAGTTCTTGG+TGG | 0.505745 | 3.3:+72548635 | MS.gene75495:CDS |
| TAATTTCCGAGCTTATGCCT+AGG | 0.515468 | 3.3:+72544167 | MS.gene75495:CDS |
| ACCAAATTCTGCAGGTGGAA+AGG | 0.517704 | 3.3:+72552063 | MS.gene75495:intron |
| TAACAAATTACCTTCTTCAG+TGG | 0.523966 | 3.3:-72551990 | None:intergenic |
| GATTTCTTCTCCGTTTGTTG+AGG | 0.532183 | 3.3:+72544287 | MS.gene75495:intron |
| AATGAAAACATAGCCATGAT+CGG | 0.532311 | 3.3:+72546162 | MS.gene75495:CDS |
| GAAAGAGCATGAAATTAGCT+AGG | 0.532821 | 3.3:-72544099 | None:intergenic |
| TAGTCACAAGTGGTCCAAAT+GGG | 0.534054 | 3.3:-72548058 | None:intergenic |
| AATTTCCGAGCTTATGCCTA+GGG | 0.534663 | 3.3:+72544168 | MS.gene75495:CDS |
| AAGGTATCAGAGCACCCTCT+TGG | 0.536712 | 3.3:+72552082 | MS.gene75495:CDS |
| TGGTAGCTCAGAGATAACTT+TGG | 0.538447 | 3.3:-72551950 | None:intergenic |
| TGTGCCGAAGCCACTGAAGA+AGG | 0.539716 | 3.3:+72551980 | MS.gene75495:CDS |
| AAGAATTTGAAGATCGATTG+TGG | 0.540222 | 3.3:-72546828 | None:intergenic |
| CTCTTTCTGTTAGAACCTTG+CGG | 0.540977 | 3.3:+72544115 | MS.gene75495:CDS |
| TCTTATTGATGATGTGATGA+AGG | 0.542228 | 3.3:+72548397 | MS.gene75495:CDS |
| ATGACAGAGCTTTCTTTCAA+GGG | 0.544675 | 3.3:-72548242 | None:intergenic |
| AAACATAGCCATGATCGGGC+TGG | 0.546841 | 3.3:+72546167 | MS.gene75495:CDS |
| GTTTCATTCATGGATTCTCA+CGG | 0.555421 | 3.3:+72544037 | MS.gene75495:CDS |
| ATATACTTACAAAATCCTCA+AGG | 0.561182 | 3.3:-72546215 | None:intergenic |
| TAAGCAGCATTTGTCGTCGA+TGG | 0.562579 | 3.3:-72544146 | None:intergenic |
| TGCACTGATAAACGAAGAAG+AGG | 0.571108 | 3.3:+72548264 | MS.gene75495:CDS |
| AAACTCCATATGCAACTCCT+CGG | 0.572586 | 3.3:-72548610 | None:intergenic |
| GCATGAAATTAGCTAGGAAG+CGG | 0.575583 | 3.3:-72544093 | None:intergenic |
| AAACTAGAATGGAAGAGCTA+CGG | 0.576554 | 3.3:+72543990 | None:intergenic |
| TCCAACTGTGATTCAAGATG+AGG | 0.586293 | 3.3:+72552117 | MS.gene75495:CDS |
| TAACTGAAGAACTTAGACAC+GGG | 0.593865 | 3.3:+72548206 | MS.gene75495:CDS |
| CAGAGCACCCTCTTGGTACG+TGG | 0.604824 | 3.3:+72552089 | MS.gene75495:CDS |
| TGATACGCATCCTCAACAAA+CGG | 0.605354 | 3.3:-72544297 | None:intergenic |
| AGCATTTGTCGTCGATGGCG+AGG | 0.608319 | 3.3:-72544141 | None:intergenic |
| AAAGGCAAATTCTACCCTTG+AGG | 0.610363 | 3.3:+72546200 | MS.gene75495:CDS |
| CTTCTTCTCTATCAACTGCA+AGG | 0.611861 | 3.3:+72548458 | MS.gene75495:CDS |
| GAAGCGGTTACAGTGATGAT+CGG | 0.615210 | 3.3:-72544077 | None:intergenic |
| TATACTTACAAAATCCTCAA+GGG | 0.623941 | 3.3:-72546214 | None:intergenic |
| GTTGCAGAACAAGAGAGTAA+TGG | 0.624688 | 3.3:+72544327 | MS.gene75495:CDS |
| ACTGGAGAAGGTACAACGCA+TGG | 0.627019 | 3.3:+72544015 | MS.gene75495:CDS |
| TTAGGCAAAGTGCATTACTG+AGG | 0.636995 | 3.3:+72551889 | MS.gene75495:intron |
| TTTGACATACCAACATAGAA+GGG | 0.652525 | 3.3:-72549542 | None:intergenic |
| TCGATGGCGAGGTCACCGCA+AGG | 0.655488 | 3.3:-72544130 | None:intergenic |
| ACTGACCCTAGGCATAAGCT+CGG | 0.665924 | 3.3:-72544173 | None:intergenic |
| TTGACATACCAACATAGAAG+GGG | 0.668333 | 3.3:-72549541 | None:intergenic |
| ATTTGCTGCAAACACCAACA+AGG | 0.670765 | 3.3:+72547902 | MS.gene75495:CDS |
| GGAATACACCACGTACCAAG+AGG | 0.678597 | 3.3:-72552097 | None:intergenic |
| AGAGCTACGGAAACTGGAGA+AGG | 0.679834 | 3.3:+72544003 | MS.gene75495:CDS |
| AACACCAACAAGGAAAGATG+AGG | 0.680119 | 3.3:+72547912 | MS.gene75495:CDS |
| GAATACACCACGTACCAAGA+GGG | 0.698160 | 3.3:-72552096 | None:intergenic |
| ATAACTGAAGAACTTAGACA+CGG | 0.701868 | 3.3:+72548205 | MS.gene75495:CDS |
| AACTGAAGAACTTAGACACG+GGG | 0.713202 | 3.3:+72548207 | MS.gene75495:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 72543998 | 72552177 | 72543998 | ID=MS.gene75495 |
| chr3.3 | mRNA | 72543998 | 72552177 | 72543998 | ID=MS.gene75495.t1;Parent=MS.gene75495 |
| chr3.3 | exon | 72543998 | 72544189 | 72543998 | ID=MS.gene75495.t1.exon1;Parent=MS.gene75495.t1 |
| chr3.3 | CDS | 72543998 | 72544189 | 72543998 | ID=cds.MS.gene75495.t1;Parent=MS.gene75495.t1 |
| chr3.3 | exon | 72544288 | 72544348 | 72544288 | ID=MS.gene75495.t1.exon2;Parent=MS.gene75495.t1 |
| chr3.3 | CDS | 72544288 | 72544348 | 72544288 | ID=cds.MS.gene75495.t1;Parent=MS.gene75495.t1 |
| chr3.3 | exon | 72546085 | 72546227 | 72546085 | ID=MS.gene75495.t1.exon3;Parent=MS.gene75495.t1 |
| chr3.3 | CDS | 72546085 | 72546227 | 72546085 | ID=cds.MS.gene75495.t1;Parent=MS.gene75495.t1 |
| chr3.3 | exon | 72546786 | 72546887 | 72546786 | ID=MS.gene75495.t1.exon4;Parent=MS.gene75495.t1 |
| chr3.3 | CDS | 72546786 | 72546887 | 72546786 | ID=cds.MS.gene75495.t1;Parent=MS.gene75495.t1 |
| chr3.3 | exon | 72547883 | 72547933 | 72547883 | ID=MS.gene75495.t1.exon5;Parent=MS.gene75495.t1 |
| chr3.3 | CDS | 72547883 | 72547933 | 72547883 | ID=cds.MS.gene75495.t1;Parent=MS.gene75495.t1 |
| chr3.3 | exon | 72548020 | 72548111 | 72548020 | ID=MS.gene75495.t1.exon6;Parent=MS.gene75495.t1 |
| chr3.3 | CDS | 72548020 | 72548111 | 72548020 | ID=cds.MS.gene75495.t1;Parent=MS.gene75495.t1 |
| chr3.3 | exon | 72548204 | 72548285 | 72548204 | ID=MS.gene75495.t1.exon7;Parent=MS.gene75495.t1 |
| chr3.3 | CDS | 72548204 | 72548285 | 72548204 | ID=cds.MS.gene75495.t1;Parent=MS.gene75495.t1 |
| chr3.3 | exon | 72548395 | 72548487 | 72548395 | ID=MS.gene75495.t1.exon8;Parent=MS.gene75495.t1 |
| chr3.3 | CDS | 72548395 | 72548487 | 72548395 | ID=cds.MS.gene75495.t1;Parent=MS.gene75495.t1 |
| chr3.3 | exon | 72548609 | 72548673 | 72548609 | ID=MS.gene75495.t1.exon9;Parent=MS.gene75495.t1 |
| chr3.3 | CDS | 72548609 | 72548673 | 72548609 | ID=cds.MS.gene75495.t1;Parent=MS.gene75495.t1 |
| chr3.3 | exon | 72549385 | 72549397 | 72549385 | ID=MS.gene75495.t1.exon10;Parent=MS.gene75495.t1 |
| chr3.3 | CDS | 72549385 | 72549397 | 72549385 | ID=cds.MS.gene75495.t1;Parent=MS.gene75495.t1 |
| chr3.3 | exon | 72549474 | 72549554 | 72549474 | ID=MS.gene75495.t1.exon11;Parent=MS.gene75495.t1 |
| chr3.3 | CDS | 72549474 | 72549554 | 72549474 | ID=cds.MS.gene75495.t1;Parent=MS.gene75495.t1 |
| chr3.3 | exon | 72551893 | 72552001 | 72551893 | ID=MS.gene75495.t1.exon12;Parent=MS.gene75495.t1 |
| chr3.3 | CDS | 72551893 | 72552001 | 72551893 | ID=cds.MS.gene75495.t1;Parent=MS.gene75495.t1 |
| chr3.3 | exon | 72552077 | 72552177 | 72552077 | ID=MS.gene75495.t1.exon13;Parent=MS.gene75495.t1 |
| chr3.3 | CDS | 72552077 | 72552177 | 72552077 | ID=cds.MS.gene75495.t1;Parent=MS.gene75495.t1 |
| Gene Sequence |
| Protein sequence |
