AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene75654

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene75654.t1 MTR_3g084630 98.582 282 4 0 1 282 1 282 0.0 583
MS.gene75654.t1 MTR_3g084620 98.227 282 5 0 1 282 1 282 0.0 580
MS.gene75654.t1 MTR_8g102220 86.170 282 26 3 1 281 1 270 6.71e-179 494
MS.gene75654.t1 MTR_8g081040 86.245 269 35 2 10 277 14 281 7.16e-178 491
MS.gene75654.t1 MTR_4g128410 72.426 272 67 2 14 278 11 281 8.19e-144 405
MS.gene75654.t1 MTR_7g093530 72.593 270 68 1 14 277 10 279 8.96e-142 400
MS.gene75654.t1 MTR_4g128580 72.285 267 64 1 21 277 19 285 1.94e-139 394
MS.gene75654.t1 MTR_4g128570 68.382 272 77 2 7 277 9 272 2.60e-137 389
MS.gene75654.t1 MTR_4g128400 63.958 283 96 1 1 277 1 283 4.71e-133 378
MS.gene75654.t1 MTR_2g038860 65.468 278 85 1 11 277 9 286 2.35e-132 377
MS.gene75654.t1 MTR_2g038705 65.468 278 85 1 11 277 9 286 2.35e-132 377
MS.gene75654.t1 MTR_4g126920 67.681 263 78 1 22 277 19 281 5.27e-131 373
MS.gene75654.t1 MTR_4g128590 64.948 291 72 4 1 277 1 275 3.23e-130 370
MS.gene75654.t1 MTR_5g091730 52.465 284 132 2 1 281 1 284 1.92e-106 311
MS.gene75654.t1 MTR_7g084770 52.740 292 126 3 1 281 1 291 2.53e-106 311
MS.gene75654.t1 MTR_7g084760 52.069 290 127 3 1 279 1 289 1.18e-104 306
MS.gene75654.t1 MTR_6g033055 51.712 292 123 4 7 281 1 291 7.07e-104 304
MS.gene75654.t1 MTR_7g084750 50.342 292 133 3 1 281 1 291 1.14e-102 301
MS.gene75654.t1 MTR_6g033085 51.027 292 131 3 1 281 1 291 1.35e-102 301
MS.gene75654.t1 MTR_8g102910 51.748 286 123 5 10 282 1 284 2.31e-101 298
MS.gene75654.t1 MTR_6g088320 49.291 282 128 3 14 282 9 288 2.55e-97 288
MS.gene75654.t1 MTR_3g089540 51.351 259 119 2 26 279 28 284 2.90e-96 285
MS.gene75654.t1 MTR_4g045853 49.286 280 122 5 14 277 13 288 9.09e-95 281
MS.gene75654.t1 MTR_2g089140 47.797 295 128 7 1 279 4 288 3.05e-94 280
MS.gene75654.t1 MTR_1g105900 47.810 274 133 2 17 282 25 296 9.37e-94 279
MS.gene75654.t1 MTR_4g097640 48.387 279 135 4 6 277 11 287 1.60e-93 278
MS.gene75654.t1 MTR_4g097670 48.387 279 135 4 6 277 11 287 1.60e-93 278
MS.gene75654.t1 MTR_4g097700 50.193 259 121 3 26 277 67 324 2.50e-92 276
MS.gene75654.t1 MTR_1g105495 44.604 278 142 3 11 277 18 294 7.36e-84 254
MS.gene75654.t1 MTR_2g089140 52.212 226 92 4 69 279 1 225 3.00e-83 249
MS.gene75654.t1 MTR_8g013055 45.000 260 128 4 28 277 31 285 1.97e-78 239
MS.gene75654.t1 MTR_7g102260 41.569 255 133 5 25 268 34 283 8.94e-63 199
MS.gene75654.t1 MTR_7g056090 39.850 266 145 6 11 265 12 273 5.13e-62 197
MS.gene75654.t1 MTR_2g095800 40.684 263 137 6 18 268 46 301 5.25e-60 193
MS.gene75654.t1 MTR_4g057450 41.603 262 136 6 27 277 49 304 5.65e-60 193
MS.gene75654.t1 MTR_1g063020 39.773 264 142 6 25 277 37 294 7.60e-59 189
MS.gene75654.t1 MTR_2g049130 38.610 259 142 7 28 278 29 278 7.85e-56 181
MS.gene75654.t1 MTR_1g033420 37.931 261 149 6 28 277 33 291 2.13e-52 174
MS.gene75654.t1 MTR_2g049030 35.714 252 148 6 28 271 30 275 4.37e-51 169
MS.gene75654.t1 MTR_5g029100 36.090 266 152 7 28 277 42 305 1.95e-50 169
MS.gene75654.t1 MTR_8g064180 35.338 266 149 8 32 278 50 311 4.54e-50 167
MS.gene75654.t1 MTR_5g029100 34.749 259 145 8 41 277 18 274 4.99e-46 157
MS.gene75654.t1 MTR_8g090100 34.601 263 157 5 28 277 21 281 2.86e-44 153
MS.gene75654.t1 MTR_8g479340 30.256 195 55 8 97 277 8 135 2.22e-16 74.7
MS.gene75654.t1 MTR_8g032640 32.558 172 108 5 50 219 33 198 1.09e-13 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene75654.t1 AT3G23730 72.028 286 69 2 7 281 5 290 2.31e-164 457
MS.gene75654.t1 AT4G14130 73.264 288 70 2 1 281 1 288 2.79e-163 455
MS.gene75654.t1 AT4G25810 65.455 275 87 1 11 277 9 283 1.39e-133 379
MS.gene75654.t1 AT4G30270 64.885 262 84 1 16 277 12 265 2.16e-129 368
MS.gene75654.t1 AT5G57550 65.370 257 86 1 25 278 27 283 4.90e-129 368
MS.gene75654.t1 AT5G57560 62.455 277 91 2 10 277 9 281 6.03e-129 368
MS.gene75654.t1 AT5G57530 63.258 264 81 2 25 277 24 282 6.21e-124 355
MS.gene75654.t1 AT5G57540 62.500 264 83 3 25 277 23 281 2.82e-122 351
MS.gene75654.t1 AT2G18800 57.857 280 99 2 17 277 17 296 5.82e-121 348
MS.gene75654.t1 AT4G25820 62.782 266 91 2 20 278 22 286 6.28e-120 345
MS.gene75654.t1 AT5G48070 57.934 271 111 2 10 277 11 281 6.21e-118 340
MS.gene75654.t1 AT4G30290 58.303 271 110 2 10 277 6 276 8.42e-110 319
MS.gene75654.t1 AT4G30280 57.778 270 111 2 11 277 12 281 2.37e-109 318
MS.gene75654.t1 AT1G65310 60.000 260 101 2 21 277 44 303 2.22e-108 316
MS.gene75654.t1 AT1G65310 60.000 260 101 2 21 277 22 281 2.37e-108 315
MS.gene75654.t1 AT5G13870 54.406 261 111 2 28 281 32 291 1.03e-101 299
MS.gene75654.t1 AT5G13870 54.406 261 111 2 28 281 32 291 1.03e-101 299
MS.gene75654.t1 AT5G13870 54.406 261 111 2 28 281 32 291 1.03e-101 299
MS.gene75654.t1 AT2G06850 52.281 285 122 3 10 281 11 294 7.66e-101 297
MS.gene75654.t1 AT4G03210 48.936 282 130 5 10 281 11 288 2.34e-96 285
MS.gene75654.t1 AT2G14620 46.181 288 140 2 10 282 12 299 8.33e-94 279
MS.gene75654.t1 AT2G06850 57.143 231 91 2 58 281 5 234 9.47e-93 274
MS.gene75654.t1 AT4G28850 47.902 286 133 5 10 282 10 292 9.89e-93 276
MS.gene75654.t1 AT5G65730 48.561 278 127 6 11 278 20 291 1.27e-91 273
MS.gene75654.t1 AT4G37800 48.120 266 126 4 21 277 27 289 7.41e-89 266
MS.gene75654.t1 AT4G03210 52.381 231 100 3 60 281 31 260 9.40e-87 260
MS.gene75654.t1 AT1G11545 45.357 280 139 4 11 277 21 299 2.43e-86 260
MS.gene75654.t1 AT2G14620 48.707 232 111 1 59 282 18 249 1.90e-82 248
MS.gene75654.t1 AT2G14620 49.327 223 105 1 68 282 16 238 2.45e-80 243
MS.gene75654.t1 AT4G13090 40.893 291 151 4 7 278 1 289 3.45e-75 231
MS.gene75654.t1 AT3G25050 41.313 259 139 3 28 277 35 289 3.17e-74 229
MS.gene75654.t1 AT4G13080 45.420 262 124 7 28 278 39 292 1.65e-72 224
MS.gene75654.t1 AT2G36870 42.857 266 135 6 25 279 40 299 2.74e-63 201
MS.gene75654.t1 AT3G44990 40.590 271 142 8 18 277 31 293 6.52e-58 187
MS.gene75654.t1 AT1G14720 34.783 299 170 7 7 281 4 301 1.91e-53 177
MS.gene75654.t1 AT1G32170 34.471 293 163 8 8 277 7 293 4.11e-53 176
MS.gene75654.t1 AT1G10550 36.029 272 139 8 32 277 47 309 4.45e-53 175
MS.gene75654.t1 AT2G01850 31.773 299 179 6 7 281 4 301 2.96e-50 169
MS.gene75654.t1 AT4G18990 34.601 263 140 7 50 282 62 322 1.64e-44 154
MS.gene75654.t1 AT3G48580 34.008 247 145 7 35 277 41 273 3.08e-43 149
MS.gene75654.t1 AT4G18990 33.755 237 121 8 77 282 17 248 4.15e-37 133
MS.gene75654.t1 AT3G48580 32.143 224 135 6 58 277 4 214 1.50e-36 130
MS.gene75654.t1 AT2G36870 43.262 141 70 3 25 157 40 178 2.51e-30 112
MS.gene75654.t1 AT3G48580 30.952 252 126 10 35 277 41 253 7.58e-28 108

Find 69 sgRNAs with CRISPR-Local

Find 93 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCTCATTGGTTGGTTCTT+TGG 0.220550 3.3:+70819676 None:intergenic
TTCTCATTGGTTGGTTCTTT+GGG 0.221209 3.3:+70819677 None:intergenic
TCGAAGAAAGAGTATTTATT+TGG 0.223648 3.3:-70820652 MS.gene75654:CDS
GCATTCTTGAAGATCCTTAT+AGG 0.241455 3.3:+70819721 None:intergenic
ATTAATATGGCTTCTTCTAT+TGG 0.241934 3.3:-70820847 None:intergenic
CACTCTGCAGGAATACCTTC+TGG 0.325804 3.3:+70819403 None:intergenic
CATTCTTGAAGATCCTTATA+GGG 0.350905 3.3:+70819722 None:intergenic
TAGTTGATAACATCCCTATA+AGG 0.361735 3.3:-70819735 MS.gene75654:CDS
GTCTCTGGAACGCTGATGAT+TGG 0.365899 3.3:-70819645 MS.gene75654:CDS
TATTACTTACATAATGTGTT+GGG 0.366572 3.3:+70819840 None:intergenic
TTGCGGTAGTAAGCTGTAAA+TGG 0.368145 3.3:+70819580 None:intergenic
ATGCAACTCAAACTCGTTGC+TGG 0.383246 3.3:-70820619 MS.gene75654:CDS
GAGAACAACAATTCTACCTT+TGG 0.383624 3.3:-70819909 MS.gene75654:CDS
ACCAAGACTTTGATCTAACT+TGG 0.385781 3.3:-70820756 MS.gene75654:CDS
ATTGGTTGGTTCTTTGGGAA+TGG 0.395735 3.3:+70819682 None:intergenic
AGACTTGAATAGATTCTCAT+TGG 0.409589 3.3:+70819664 None:intergenic
ATAAATACTCTTTCTTCGAT+TGG 0.413180 3.3:+70820656 None:intergenic
TTCAAGAATGCTGAATCAAT+TGG 0.430099 3.3:-70819709 MS.gene75654:CDS
TGAGAATCTATTCAAGTCTC+TGG 0.437244 3.3:-70819660 MS.gene75654:CDS
AGAGTAAGTGTGGAAGTTCT+TGG 0.438651 3.3:+70819876 None:intergenic
CTAGACAAAACCTCGGGCTC+TGG 0.447506 3.3:-70820682 MS.gene75654:CDS
TCCACACTTACTCTATCATC+TGG 0.453939 3.3:-70819867 MS.gene75654:CDS
GTAAGCTGTAAATGGTGCTT+TGG 0.464723 3.3:+70819588 None:intergenic
TTGAATAGATTCTCATTGGT+TGG 0.466553 3.3:+70819668 None:intergenic
ATATTACTTACATAATGTGT+TGG 0.469145 3.3:+70819839 None:intergenic
TCTCTGGAACGCTGATGATT+GGG 0.473167 3.3:-70819644 MS.gene75654:CDS
AGCAATGAGCTTGATGCATA+TGG 0.481427 3.3:-70819496 MS.gene75654:CDS
AGTCATGATAATTAGCTCCT+TGG 0.482305 3.3:-70820803 MS.gene75654:CDS
CTCGTTGCTGGAAACTCCGC+TGG 0.483628 3.3:-70820607 MS.gene75654:CDS
TCATTGATCTCTCAGAACCT+TGG 0.486786 3.3:+70819373 None:intergenic
CTTGGACGCTTGCACTCTGC+AGG 0.489897 3.3:+70819391 None:intergenic
AAGTGTGGAAGTTCTTGGTT+GGG 0.498153 3.3:+70819881 None:intergenic
CTGCAGAGTGCAAGCGTCCA+AGG 0.505272 3.3:-70819390 MS.gene75654:CDS
ACTCTGCAGGAATACCTTCT+GGG 0.505825 3.3:+70819404 None:intergenic
TTGGGCTACAAGAGGTGGTT+TGG 0.508778 3.3:-70819626 MS.gene75654:CDS
CCAAGACTTTGATCTAACTT+GGG 0.509119 3.3:-70820755 MS.gene75654:CDS
ACGTTTGTATGAAGAATGTA+AGG 0.516073 3.3:+70819955 None:intergenic
CACCGTGCTAAGATATTCAA+TGG 0.519496 3.3:-70820727 MS.gene75654:CDS
CCCAAGTTAGATCAAAGTCT+TGG 0.522663 3.3:+70820755 None:intergenic
GGCATGAACAATATGCTGAT+TGG 0.535335 3.3:-70820826 MS.gene75654:CDS
GAGTTCTTGGGAAATGTTAG+CGG 0.540045 3.3:-70819982 MS.gene75654:CDS
TAAGTGTGGAAGTTCTTGGT+TGG 0.544799 3.3:+70819880 None:intergenic
GTGGTTTGGTGAAAACTGAT+TGG 0.548484 3.3:-70819612 MS.gene75654:CDS
AAATCTATTTCATCGTGTGT+TGG 0.556214 3.3:+70820006 None:intergenic
ACTCGGTAGCTTTGAAATTG+CGG 0.568678 3.3:+70819563 None:intergenic
ATTACTTACATAATGTGTTG+GGG 0.569004 3.3:+70819841 None:intergenic
TGCAGAATCAGAGAAGGAAG+TGG 0.572405 3.3:+70819525 None:intergenic
CGATTGGAAGCCAGAGCCCG+AGG 0.573236 3.3:+70820672 None:intergenic
GATGATTGGGCTACAAGAGG+TGG 0.583367 3.3:-70819631 MS.gene75654:CDS
TCCAGATGATAGAGTAAGTG+TGG 0.584360 3.3:+70819866 None:intergenic
ATGGTAGAAGAAGATTGAGG+TGG 0.584786 3.3:-70819477 MS.gene75654:CDS
AGCAGAACATGTAGCTACCA+AGG 0.586171 3.3:+70820786 None:intergenic
TCTTGGTTGGGTCAAACCAA+AGG 0.586504 3.3:+70819893 None:intergenic
CTTTCTCTAGACAAAACCTC+GGG 0.590397 3.3:-70820688 MS.gene75654:CDS
TCTTTCTCTAGACAAAACCT+CGG 0.610000 3.3:-70820689 MS.gene75654:CDS
CATATGGTAGAAGAAGATTG+AGG 0.622675 3.3:-70819480 MS.gene75654:CDS
CAAGACTTTGATCTAACTTG+GGG 0.623881 3.3:-70820754 MS.gene75654:CDS
TTGCAATGCAGAATCAGAGA+AGG 0.624929 3.3:+70819519 None:intergenic
CACCATTGAATATCTTAGCA+CGG 0.635606 3.3:+70820725 None:intergenic
TGGTAGAAGAAGATTGAGGT+GGG 0.642709 3.3:-70819476 MS.gene75654:CDS
ATGGTGCAGTTATCATCACA+AGG 0.643718 3.3:-70820030 MS.gene75654:intron
GCTGATGATTGGGCTACAAG+AGG 0.647339 3.3:-70819634 MS.gene75654:CDS
GATCTTAAGCGATTCCCAGA+AGG 0.649786 3.3:-70819418 MS.gene75654:CDS
GGAAGAGATAGATGATAACT+CGG 0.649852 3.3:+70819546 None:intergenic
GTAAGCAGTGACGGTTCCAG+CGG 0.667054 3.3:+70820591 None:intergenic
ACGTACGTAGTAAGCAGTGA+CGG 0.675655 3.3:+70820582 None:intergenic
CGTGCTAAGATATTCAATGG+TGG 0.692895 3.3:-70820724 MS.gene75654:CDS
TGGTGCAGTTATCATCACAA+GGG 0.717771 3.3:-70820029 MS.gene75654:intron
CATTGAATATCTTAGCACGG+TGG 0.785980 3.3:+70820728 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTCTATCTAATTAATT+CGG - chr3.3:70819671-70819690 MS.gene75654:CDS 10.0%
!! ATTAATTAGATAGAAAAAAG+AGG + chr3.3:70819670-70819689 None:intergenic 15.0%
!! GAATATAATATATAAATGTG+AGG + chr3.3:70819845-70819864 None:intergenic 15.0%
!! ATAATATATAAATGTGAGGA+AGG + chr3.3:70819841-70819860 None:intergenic 20.0%
!! ATATTACTTACATAATGTGT+TGG + chr3.3:70820389-70820408 None:intergenic 20.0%
!! TATTACTTACATAATGTGTT+GGG + chr3.3:70820388-70820407 None:intergenic 20.0%
!! TGAGATTAGAACATAAATAA+CGG + chr3.3:70819943-70819962 None:intergenic 20.0%
!!! TAGAAGTATATGATTTTCAA+TGG - chr3.3:70820176-70820195 MS.gene75654:intron 20.0%
! ATAAATACTCTTTCTTCGAT+TGG + chr3.3:70819572-70819591 None:intergenic 25.0%
! ATTACTTACATAATGTGTTG+GGG + chr3.3:70820387-70820406 None:intergenic 25.0%
! GTACAAAGTTACATATAACT+TGG + chr3.3:70820040-70820059 None:intergenic 25.0%
!! AAGTGTAGTTTTTGCAAAAA+TGG - chr3.3:70819756-70819775 MS.gene75654:intron 25.0%
!! TAACGGTTGTATTTTTCATA+TGG + chr3.3:70819926-70819945 None:intergenic 25.0%
!!! GAAATAGATTTTGAGTTCTT+GGG - chr3.3:70820231-70820250 MS.gene75654:intron 25.0%
!!! TCGAAGAAAGAGTATTTATT+TGG - chr3.3:70819573-70819592 MS.gene75654:CDS 25.0%
!!! TGAAATAGATTTTGAGTTCT+TGG - chr3.3:70820230-70820249 MS.gene75654:intron 25.0%
!!! TTACATATAACTTGGTTTGT+GGG + chr3.3:70820032-70820051 None:intergenic 25.0%
AAATCTATTTCATCGTGTGT+TGG + chr3.3:70820222-70820241 None:intergenic 30.0%
ACGTTTGTATGAAGAATGTA+AGG + chr3.3:70820273-70820292 None:intergenic 30.0%
AGACTTGAATAGATTCTCAT+TGG + chr3.3:70820564-70820583 None:intergenic 30.0%
CATTCTTGAAGATCCTTATA+GGG + chr3.3:70820506-70820525 None:intergenic 30.0%
TGTCGTGAATGCATAAATAA+CGG + chr3.3:70819988-70820007 None:intergenic 30.0%
TTCAAGAATGCTGAATCAAT+TGG - chr3.3:70820516-70820535 MS.gene75654:intron 30.0%
TTGAATAGATTCTCATTGGT+TGG + chr3.3:70820560-70820579 None:intergenic 30.0%
TTGCAAAATCATTGTAACAC+TGG - chr3.3:70819719-70819738 MS.gene75654:CDS 30.0%
TTTCAAAAGCAACTTTACCT+TGG - chr3.3:70819871-70819890 MS.gene75654:CDS 30.0%
! GTTACATATAACTTGGTTTG+TGG + chr3.3:70820033-70820052 None:intergenic 30.0%
! TGTAGTTTTTGCAAAAATGG+TGG - chr3.3:70819759-70819778 MS.gene75654:intron 30.0%
!! AATTCGGTGTATGATTAGAT+TGG - chr3.3:70819687-70819706 MS.gene75654:CDS 30.0%
!! TAGTTGATAACATCCCTATA+AGG - chr3.3:70820490-70820509 MS.gene75654:intron 30.0%
!! TAGTTTGCTTCTTTGTATTG+TGG - chr3.3:70820141-70820160 MS.gene75654:intron 30.0%
ACCAAGACTTTGATCTAACT+TGG - chr3.3:70819469-70819488 MS.gene75654:CDS 35.0%
AGTCATGATAATTAGCTCCT+TGG - chr3.3:70819422-70819441 MS.gene75654:CDS 35.0%
CAAGACTTTGATCTAACTTG+GGG - chr3.3:70819471-70819490 MS.gene75654:CDS 35.0%
CACCATTGAATATCTTAGCA+CGG + chr3.3:70819503-70819522 None:intergenic 35.0%
CATATGGTAGAAGAAGATTG+AGG - chr3.3:70820745-70820764 MS.gene75654:CDS 35.0%
CATTTGATATCAAGAAGCCA+AGG + chr3.3:70819891-70819910 None:intergenic 35.0%
CCAAGACTTTGATCTAACTT+GGG - chr3.3:70819470-70819489 MS.gene75654:CDS 35.0%
GAGAACAACAATTCTACCTT+TGG - chr3.3:70820316-70820335 MS.gene75654:intron 35.0%
GCATTCTTGAAGATCCTTAT+AGG + chr3.3:70820507-70820526 None:intergenic 35.0%
GGAAGAGATAGATGATAACT+CGG + chr3.3:70820682-70820701 None:intergenic 35.0%
TATATAAATGTGAGGAAGGC+AGG + chr3.3:70819837-70819856 None:intergenic 35.0%
TCTTTCTCTAGACAAAACCT+CGG - chr3.3:70819536-70819555 MS.gene75654:CDS 35.0%
TGAGAATCTATTCAAGTCTC+TGG - chr3.3:70820565-70820584 MS.gene75654:intron 35.0%
! AGTTTTTGCAAAAATGGTGG+TGG - chr3.3:70819762-70819781 MS.gene75654:intron 35.0%
! ATTCTCATTGGTTGGTTCTT+TGG + chr3.3:70820552-70820571 None:intergenic 35.0%
! CATACAAACGTGTTTTCTCA+AGG - chr3.3:70820282-70820301 MS.gene75654:intron 35.0%
! TTCTCATTGGTTGGTTCTTT+GGG + chr3.3:70820551-70820570 None:intergenic 35.0%
!! TATAACTTGGTTTGTGGGTT+TGG + chr3.3:70820027-70820046 None:intergenic 35.0%
AAGTGTGGAAGTTCTTGGTT+GGG + chr3.3:70820347-70820366 None:intergenic 40.0%
AGAGTAAGTGTGGAAGTTCT+TGG + chr3.3:70820352-70820371 None:intergenic 40.0%
AGCAATGAGCTTGATGCATA+TGG - chr3.3:70820729-70820748 MS.gene75654:CDS 40.0%
ATGGTAGAAGAAGATTGAGG+TGG - chr3.3:70820748-70820767 MS.gene75654:CDS 40.0%
ATGGTGCAGTTATCATCACA+AGG - chr3.3:70820195-70820214 MS.gene75654:intron 40.0%
CACCGTGCTAAGATATTCAA+TGG - chr3.3:70819498-70819517 MS.gene75654:CDS 40.0%
CATTGAATATCTTAGCACGG+TGG + chr3.3:70819500-70819519 None:intergenic 40.0%
CCCAAGTTAGATCAAAGTCT+TGG + chr3.3:70819473-70819492 None:intergenic 40.0%
CGTGCTAAGATATTCAATGG+TGG - chr3.3:70819501-70819520 MS.gene75654:CDS 40.0%
CTTTCTCTAGACAAAACCTC+GGG - chr3.3:70819537-70819556 MS.gene75654:CDS 40.0%
GAGTTCTTGGGAAATGTTAG+CGG - chr3.3:70820243-70820262 MS.gene75654:intron 40.0%
GGCATGAACAATATGCTGAT+TGG - chr3.3:70819399-70819418 MS.gene75654:CDS 40.0%
GTAAGCTGTAAATGGTGCTT+TGG + chr3.3:70820640-70820659 None:intergenic 40.0%
TAAGTGTGGAAGTTCTTGGT+TGG + chr3.3:70820348-70820367 None:intergenic 40.0%
TCCACACTTACTCTATCATC+TGG - chr3.3:70820358-70820377 MS.gene75654:intron 40.0%
TCCAGATGATAGAGTAAGTG+TGG + chr3.3:70820362-70820381 None:intergenic 40.0%
TGGTAGAAGAAGATTGAGGT+GGG - chr3.3:70820749-70820768 MS.gene75654:CDS 40.0%
TGGTGCAGTTATCATCACAA+GGG - chr3.3:70820196-70820215 MS.gene75654:intron 40.0%
TTGCAATGCAGAATCAGAGA+AGG + chr3.3:70820709-70820728 None:intergenic 40.0%
TTGCGGTAGTAAGCTGTAAA+TGG + chr3.3:70820648-70820667 None:intergenic 40.0%
! AACGTGTTTTCTCAAGGCAA+AGG - chr3.3:70820288-70820307 MS.gene75654:intron 40.0%
! ATTGGTTGGTTCTTTGGGAA+TGG + chr3.3:70820546-70820565 None:intergenic 40.0%
!! ACTCGGTAGCTTTGAAATTG+CGG + chr3.3:70820665-70820684 None:intergenic 40.0%
!! GTGGTTTGGTGAAAACTGAT+TGG - chr3.3:70820613-70820632 MS.gene75654:CDS 40.0%
ACGTACGTAGTAAGCAGTGA+CGG + chr3.3:70819646-70819665 None:intergenic 45.0%
ACTCTGCAGGAATACCTTCT+GGG + chr3.3:70820824-70820843 None:intergenic 45.0%
AGCAGAACATGTAGCTACCA+AGG + chr3.3:70819442-70819461 None:intergenic 45.0%
ATGCAACTCAAACTCGTTGC+TGG - chr3.3:70819606-70819625 MS.gene75654:CDS 45.0%
GATCTTAAGCGATTCCCAGA+AGG - chr3.3:70820807-70820826 MS.gene75654:CDS 45.0%
TCTTGGTTGGGTCAAACCAA+AGG + chr3.3:70820335-70820354 None:intergenic 45.0%
TGCAGAATCAGAGAAGGAAG+TGG + chr3.3:70820703-70820722 None:intergenic 45.0%
!! TCTCTGGAACGCTGATGATT+GGG - chr3.3:70820581-70820600 MS.gene75654:intron 45.0%
CACTCTGCAGGAATACCTTC+TGG + chr3.3:70820825-70820844 None:intergenic 50.0%
GATGATTGGGCTACAAGAGG+TGG - chr3.3:70820594-70820613 MS.gene75654:CDS 50.0%
GCTGATGATTGGGCTACAAG+AGG - chr3.3:70820591-70820610 MS.gene75654:CDS 50.0%
TTGGGCTACAAGAGGTGGTT+TGG - chr3.3:70820599-70820618 MS.gene75654:CDS 50.0%
!! CTTGGTTTGTGGGTTTGGTG+CGG + chr3.3:70820022-70820041 None:intergenic 50.0%
!! GTCTCTGGAACGCTGATGAT+TGG - chr3.3:70820580-70820599 MS.gene75654:intron 50.0%
CTAGACAAAACCTCGGGCTC+TGG - chr3.3:70819543-70819562 MS.gene75654:CDS 55.0%
GTAAGCAGTGACGGTTCCAG+CGG + chr3.3:70819637-70819656 None:intergenic 55.0%
CTCGTTGCTGGAAACTCCGC+TGG - chr3.3:70819618-70819637 MS.gene75654:CDS 60.0%
CTGCAGAGTGCAAGCGTCCA+AGG - chr3.3:70820835-70820854 MS.gene75654:CDS 60.0%
CTTGGACGCTTGCACTCTGC+AGG + chr3.3:70820837-70820856 None:intergenic 60.0%
CGATTGGAAGCCAGAGCCCG+AGG + chr3.3:70819556-70819575 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 70819384 70820863 70819384 ID=MS.gene75654
chr3.3 mRNA 70819384 70820863 70819384 ID=MS.gene75654.t1;Parent=MS.gene75654
chr3.3 exon 70820588 70820863 70820588 ID=MS.gene75654.t1.exon1;Parent=MS.gene75654.t1
chr3.3 CDS 70820588 70820863 70820588 ID=cds.MS.gene75654.t1;Parent=MS.gene75654.t1
chr3.3 exon 70819850 70820043 70819850 ID=MS.gene75654.t1.exon2;Parent=MS.gene75654.t1
chr3.3 CDS 70819850 70820043 70819850 ID=cds.MS.gene75654.t1;Parent=MS.gene75654.t1
chr3.3 exon 70819384 70819762 70819384 ID=MS.gene75654.t1.exon3;Parent=MS.gene75654.t1
chr3.3 CDS 70819384 70819762 70819384 ID=cds.MS.gene75654.t1;Parent=MS.gene75654.t1
Gene Sequence

>MS.gene75654

ATGGCTTCTTCTATTGGCATGAACAATATGCTGATTGGAGTCATGATAATTAGCTCCTTGGTAGCTACATGTTCTGCTAACTTCAACCAAGACTTTGATCTAACTTGGGGTGACCACCGTGCTAAGATATTCAATGGTGGTCAACTTCTATCTCTTTCTCTAGACAAAACCTCGGGCTCTGGCTTCCAATCGAAGAAAGAGTATTTATTTGGTAGAATTGATATGCAACTCAAACTCGTTGCTGGAAACTCCGCTGGAACCGTCACTGCTTACTACGTACGTACCTCTTTTTTCTATCTAATTAATTCGGTGTATGATTAGATTGGTTGTGTGTTTTGCAAAATCATTGTAACACTGGTTTCTGAAACTATGAAGTGTAGTTTTTGCAAAAATGGTGGTGGCTCACAATGATTTTCACAAACGTATAAATGCATATAGTTTGATTCTTTTCCTGCCTTCCTCACATTTATATATTATATTCTCTCTTTTTCAAAAGCAACTTTACCTTGGCTTCTTGATATCAAATGCATTAATTTTCTCCATATGAAAAATACAACCGTTATTTATGTTCTAATCTCATTAAATATAACTGTTATTTGAACCGTTATTTATGCATTCACGACATATAAACTCTACCGCACCAAACCCACAAACCAAGTTATATGTAACTTTGTACTAGCATACGTAATTATTTTTTCAATTTTTTTCTATATACAAACACAATGAATTTCACGAGCAATTTTTTGTGTTCTGTTTCTAGTTTGCTTCTTTGTATTGTGGTGTTGTCAATAATAGAAGTATATGATTTTCAATGGTGCAGTTATCATCACAAGGGCCAACACACGATGAAATAGATTTTGAGTTCTTGGGAAATGTTAGCGGTGATCCTTACATTCTTCATACAAACGTGTTTTCTCAAGGCAAAGGAAACAGAGAACAACAATTCTACCTTTGGTTTGACCCAACCAAGAACTTCCACACTTACTCTATCATCTGGAAGCCCCAACACATTATGTAAGTAATATATATTTAAGTTTGATCAAACAGCTCAATTAAGCGCTTATAAAAAAAACGCTAACAAGTTCGTATAACTAAATGCAGATTTTTAGTTGATAACATCCCTATAAGGATCTTCAAGAATGCTGAATCAATTGGTATTCCATTCCCAAAGAACCAACCAATGAGAATCTATTCAAGTCTCTGGAACGCTGATGATTGGGCTACAAGAGGTGGTTTGGTGAAAACTGATTGGTCCAAAGCACCATTTACAGCTTACTACCGCAATTTCAAAGCTACCGAGTTATCATCTATCTCTTCCACTTCCTTCTCTGATTCTGCATTGCAAAGCAATGAGCTTGATGCATATGGTAGAAGAAGATTGAGGTGGGTTCAGAAGTACTTCATGATCTATAACTACTGCAACGATCTTAAGCGATTCCCAGAAGGTATTCCTGCAGAGTGCAAGCGTCCAAGGTTCTGA
Protein sequence

>MS.gene75654.t1

MASSIGMNNMLIGVMIISSLVATCSANFNQDFDLTWGDHRAKIFNGGQLLSLSLDKTSGSGFQSKKEYLFGRIDMQLKLVAGNSAGTVTAYYLSSQGPTHDEIDFEFLGNVSGDPYILHTNVFSQGKGNREQQFYLWFDPTKNFHTYSIIWKPQHIIFLVDNIPIRIFKNAESIGIPFPKNQPMRIYSSLWNADDWATRGGLVKTDWSKAPFTAYYRNFKATELSSISSTSFSDSALQSNELDAYGRRRLRWVQKYFMIYNYCNDLKRFPEGIPAECKRPRF